LOCUS       NZ_CP075712             2337 bp    DNA     linear   CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC2018-607 plasmid pSTEC2018_607-o.
ACCESSION   NZ_CP075712
VERSION     NZ_CP075712.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993387
            Assembly: GCF_016403625.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 2337)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: STEC2018-607
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:12:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,293
            CDSs (with protein)               :: 5,293
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 100 of 257
            Pseudo Genes (incomplete)         :: 139 of 257
            Pseudo Genes (internal stop)      :: 55 of 257
            Pseudo Genes (multiple problems)  :: 37 of 257
            Pseudo Genes (short protein)      :: 4 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075712.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC2018-607"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC2018_607-o"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2018"
                     /collected_by="ILS/NENT"
     gene            1072..1497
                     /gene="mobA"
                     /locus_tag="JFX52_RS28025"
                     /old_locus_tag="JFX52_28025"
     CDS             1072..1497
                     /gene="mobA"
                     /locus_tag="JFX52_RS28025"
                     /old_locus_tag="JFX52_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_039023338.1"
                     /translation="MTKNKRSGSEVRQRTKVITLRVNNRIANEIRRRAKSEGLTISEYI
                     RTTSLNNEIKQRVPSRYLDELIQLGRMQKKLFDKGKRPKDKEYLDVMHKIISLCAEMKE
                     VTQRISDIYKKMDLIKEEIKAIKKIHKNKYPGSNLFN"
                     /name="mobA"
     gene            complement(1587..2000)
                     /locus_tag="JFX52_RS28030"
                     /old_locus_tag="JFX52_28030"
     CDS             complement(1587..2000)
                     /locus_tag="JFX52_RS28030"
                     /old_locus_tag="JFX52_28030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_028132811.1"
                     /translation="MGAKFLDQKTGVILEEPEFVKLYIRDLCRVKGLSATQYKIFTFML
                     ANMNTDNVVSFGPRTKEKFLSEHSIKPQTFNNNVSQLIESCLISRIARNEFLVNKKYAV
                     KVDWAKVQKIVWTTTYSNEGIDSTVTIAPKKTD"
                     /name="hypothetical protein"
     Typing          31..289
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.528"
                     /coverage="100.0"
                     /evalue="1.5e-65"
ORIGIN
        1 cggtcgctgg ggctgcggcg agcggtgcca cctctcgcat atcgttgacg atcccgattt
       61 gatctcaggc gatcgagtcg ctggccaccg gttaaacgcg ctcaaagcct cccagacgca
      121 caaaacgggg gttaaatgcc cgtcagggca tggaaggcgc gaaaaacgct tacagaggtt
      181 ttcagaaggt taggcgcgcc gataccccta accaggggct ttcaggtcgc taggaaccgc
      241 gccagccccc tgcaaggccc atttgcacgg aacagacagc cactcggttg gtaggtggtc
      301 gtagaccgtc ggtgttacag cgatgggggt ctggggttct ccccagacgg tttccggttg
      361 cgctcaaggc gctgccggaa ccgtggcggc ttcgatggtg aagggtgagc gcgtttccaa
      421 gaggttttcc aggcaagcta tgactgaact gaacagattg aacgaggagg aaacgcgctc
      481 agacgctttg tagcgcgctt ttctgtgtca ggcaagtttg ttgtgtcttt tgcctttcag
      541 gttgttagca gctggcggag cactgcacgg ggattaatgc tcccattgct aggatgaaat
      601 aaagattagg agcgaagtta agttatattt ttaatatata aacgcgatat caataactca
      661 catagttata ttttatgcat atgctaccat cttgctcttt tgcctcctgc tctctggacg
      721 aatcccgcat taccaacagg caatggatta tataaccaag aagatctaat ctttattcct
      781 ctaataatcc acaaacgaca gacaacaaag acatcaatta attcggtgtt atttattaaa
      841 taacaagcaa taatttttaa aatctgtgta aataatattg aatgaagcat catttttttg
      901 tatcagaaat accaggttac gacgcggtat tttattttcg tcgaaaagtt gcagcggaaa
      961 atgggacagg atgtgtattc gtcataccgc gaatacattc gcttgcatga gaatacctcc
     1021 tgtcagggca agccccgaca ccccaaacgt gcaaaatgga gaatcattgc gatgactaaa
     1081 aataagcgta gcggttcaga agtacggcag aggactaagg ttatcaccct tagagtaaat
     1141 aacagaatcg caaacgaaat cagacggcga gcgaagagcg aagggctgac tatttcagaa
     1201 tatataagga caacaagctt aaataatgaa ataaaacagc gcgttccttc cagatattta
     1261 gatgaattaa tccaacttgg tcggatgcag aagaaattat ttgataaagg gaagagaccg
     1321 aaggacaaag agtatctgga tgtgatgcat aaaataattt cactttgtgc tgaaatgaaa
     1381 gaagtaacac agcgaatttc tgatatatat aaaaaaatgg atttaataaa agaagaaata
     1441 aaagcaataa aaaaaataca taaaaataaa tacccaggtt ccaacttatt taattgagca
     1501 gaacataggt cttcataaaa atttaaagac ctataataga attagcgaca ttttgtttta
     1561 tcgaaatctt ttacaatcat aagaaattaa tccgtttttt tcggggctat cgtcactgtt
     1621 gaatcaatcc cttcattact gtaagttgtt gtccatacta tcttttgtac tttcgcccaa
     1681 tcaactttaa ctgcgtattt tttattaacc aaaaattcat ttctggctat tctagaaatt
     1741 aaacatgact caatcagttg agacacatta ttattaaatg tttgtggttt aattgaatgt
     1801 tctgataaaa acttttcttt ggttcttggt ccaaatgaaa caacattatc agtattcata
     1861 ttagcaagca tgaacgtaaa aattttgtat tgcgttgctg atagcccttt aacacggcat
     1921 aaatcacgga tatacagctt cacgaactcc ggctcttcca aaattacgcc agttttttga
     1981 tctaaaaatt tcgctcccat tctcacctcg caggtaattt agttaccgtt ttatcggtat
     2041 cttatgtatg ttccacaacc gagtaaatac tttttttccg gtatttgatt agcaacaaaa
     2101 acagtattcc ggtatttgaa ataccgctga gcggtatttc aacctccgct cagcggtaaa
     2161 aactttcccg ctgagcggta tatctattcc cgctgagcgg tatttagttt gcctctaggc
     2221 cttgtcacac ctgattttcc tatctgtctg tgaatctatc ttagatctcg tgcgtaagtg
     2281 cactatgcca atactggttc ccttccgctt cctcgcccac tcgcgagcct agcggct
//
