LOCUS       NZ_CP075711             2461 bp    DNA     linear   CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC2018-607 plasmid pSTEC2018_607-n.
ACCESSION   NZ_CP075711
VERSION     NZ_CP075711.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993387
            Assembly: GCF_016403625.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 2461)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: STEC2018-607
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:12:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,293
            CDSs (with protein)               :: 5,293
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 100 of 257
            Pseudo Genes (incomplete)         :: 139 of 257
            Pseudo Genes (internal stop)      :: 55 of 257
            Pseudo Genes (multiple problems)  :: 37 of 257
            Pseudo Genes (short protein)      :: 4 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075711.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC2018-607"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC2018_607-n"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2018"
                     /collected_by="ILS/NENT"
     gene            <1..206
                     /locus_tag="JFX52_RS28010"
                     /old_locus_tag="JFX52_28010"
     CDS             <1..206
                     /locus_tag="JFX52_RS28010"
                     /old_locus_tag="JFX52_28010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_215771243.1"
                     /translation="KRASNPPSRHGSGCALSATGNRLGRTPDPHRCNIIGLRPPTNRAA
                     ACTVQRGFAGAGAVPSDLKTPG"
                     /name="hypothetical protein"
     gene            792..1337
                     /locus_tag="JFX52_RS28015"
                     /old_locus_tag="JFX52_28015"
     CDS             792..1337
                     /locus_tag="JFX52_RS28015"
                     /old_locus_tag="JFX52_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002030412.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_021535322.1"
                     /translation="MSKSKALNINKRFSENPFIKDLIIPVKNKQVRISRLGEDNNIMIN
                     QSTGEVLGGTSVVTYKKVDSDNFIKLFTQNIKLAFELTQAGQKALFVLFWAVQNKTNND
                     LVLLDQYTLNDFINENKGLSMSLPTFKRGLGELVKSQILAMNKRKGFYYINPNFVFNGD
                     RVAFTTVLEMNKSSHKDK"
                     /name="replication/maintenance protein RepL"
     gene            complement(1417..1842)
                     /gene="mobA"
                     /locus_tag="JFX52_RS28020"
                     /old_locus_tag="JFX52_28020"
     CDS             complement(1417..1842)
                     /gene="mobA"
                     /locus_tag="JFX52_RS28020"
                     /old_locus_tag="JFX52_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_021535321.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLDELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDVYNEMDLLKQEIKIIKRHHKNKYPGSNIFS"
                     /name="mobA"
     Typing          complement(127..386)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.695"
                     /coverage="100.0"
                     /evalue="6.76e-89"
ORIGIN
        1 ggaaacgcgc ctcaaatcca ccaagccgcc acggttccgg ctgcgccttg agcgcaaccg
       61 gaaaccgact ggggagaacc ccagaccccc accgctgcaa catcatcggt ctgcgaccac
      121 ctaccaaccg agcggctgcc tgtaccgtgc aaaggggctt tgcaggggct ggcgcggttc
      181 ctagcgacct gaaaacccct ggttaggggt atcggcgcgc ctggcctcct gaaaccctct
      241 gtaagcgttt ttcgcgcctt ccatgccctg gcgggcattt aaaacccgtt tcgtgcgtct
      301 gagagcttct gagagcgttt taggggctat ctggcaactc atccccgaaa gagcaaccgc
      361 ggaatcaccg acaacatgcg agaggtaatt ccgctcgccg caagtcgatg accgagcgta
      421 gcgagcgaat cgacgaggaa gcggaagaga accggaagct acattgagca cttacgcaca
      481 gaatttggcc tacgcccccg cgtccgtgca cgcgtttaat aagaattaag aagggaaatc
      541 gcgataaacc gcgccaatcc tagaatctaa tgatcaggat attgatctaa cgtatcagaa
      601 tagagcatct aacatatcaa ttagttgata tttaaagtat tgattgtttg ttttttgctc
      661 atcgcgagtg tgatttattt gatctttaag gtatcaaaac tatgatacta gtcggatcgg
      721 tttattgaca tttgtagatt ttttcttgat agtcgaggta tcagtttatt gatacctgag
      781 agtgtaaaat aatgagcaag agcaaggctt taaacataaa caaacgattt agtgaaaatc
      841 ctttcatcaa agatttgata attcctgtaa aaaacaaaca agtaagaata tcgcgattag
      901 gagaagataa taacataatg attaaccaat caactggaga ggttttaggg ggaacctcgg
      961 tagtaactta taaaaaagtt gatagtgata attttattaa attgtttacc caaaatataa
     1021 aattggcttt cgagttaaca caagctggtc aaaaagcact atttgtttta ttttgggcag
     1081 tacaaaataa aacaaataat gatctagtct tattagatca atacacgcta aatgatttta
     1141 taaatgaaaa caaaggatta agcatgtccc ttccaacatt caaacgtggg cttggggagt
     1201 tggtaaaatc acaaatatta gcaatgaata agcgaaaggg attttattat ataaatccta
     1261 attttgtttt taatggcgat agagttgcat tcacaacagt tcttgaaatg aataaatcga
     1321 gccataaaga caaatgacaa tgtattaagc ccgtcaaagg aaaatttcaa atctattggc
     1381 gggttttaat attcgtaacg gctagcgccg aagattttag ctaaatatat ttgagccagg
     1441 gtatttattt ttatgatgcc gttttattat ttttatttcc tgttttaata gatccatttc
     1501 attatataca tcagaaattc gcttcgttac tattttcatt tcatcgcaaa gtaaaattat
     1561 tttatgcatc acctcgaggt actccttatc cttcggccgc tttcccttat caaataattt
     1621 cttctgcatc cgaccaagtc ggattaattc atctaaatac ctggaaggta cgcgctgttt
     1681 tatttcatta tttaaacttg ccgtcctgat atattctgaa attgtcagtc cttcgttctt
     1741 cgctcgccgt ctgatttcgc ttgcaactct gttatttact ctaagggtga taaccttagt
     1801 tctctgccgt acttctgaac cactacgttt atttttagcc atcgcaatga ctctccattt
     1861 tgcacgtttg gggtgtcggg gcttgccctg acaggaggta ttctcatgca agcgaatgta
     1921 ttcgcggtat gacgaataca catcctgtcc cattttccgc tacaactttt cgacgaaaat
     1981 aaaataccgc gtcgtaacct ggtatttctg atacaaaaaa acgatgcttc attcaatatt
     2041 atttacacag attttaaaag ttattgtttg ttatttaata aataacgcaa aatcaattga
     2101 aagttttgtt ctttgtcgtt tgttgattat tgagtaaata aagatcgtgt cttcctgatt
     2161 atataatcca ttgcctgtta gtgatacgta gtgtgtccaa ggaacaggaa gcggaagagc
     2221 aagatgtagc atgtgcataa aatataaata tgtgagttat tgatatcgca tttatatatt
     2281 aaaaatataa cttaacgtcg ctcttaatat ttatttcatc ctggcaatgg gagcattaat
     2341 cccgtgcagc gctccgccag ctgctgacgg cctcgacatg caaccttcaa tccgataaaa
     2401 gatcggggcg cgtttccggc aacaactaac catagggatg gtttgcttgc cgacaaacgc
     2461 c
//
