LOCUS       NZ_CP075705             7118 bp    DNA     linear   CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC2018-607 plasmid pSTEC2018_607-h.
ACCESSION   NZ_CP075705
VERSION     NZ_CP075705.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993387
            Assembly: GCF_016403625.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 7118)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: STEC2018-607
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:12:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,293
            CDSs (with protein)               :: 5,293
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 100 of 257
            Pseudo Genes (incomplete)         :: 139 of 257
            Pseudo Genes (internal stop)      :: 55 of 257
            Pseudo Genes (multiple problems)  :: 37 of 257
            Pseudo Genes (short protein)      :: 4 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075705.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC2018-607"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC2018_607-h"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2018"
                     /collected_by="ILS/NENT"
     gene            224..2443
                     /gene="mobQ"
                     /locus_tag="JFX52_RS27875"
                     /old_locus_tag="JFX52_27875"
     CDS             224..2443
                     /gene="mobQ"
                     /locus_tag="JFX52_RS27875"
                     /old_locus_tag="JFX52_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_062850873.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPENAPSWCADRSVLWNAVEKAEQRRDSQLAREIELAIPREISRDAARET
                     VLAFVRENFVSQGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKSACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRKVQETQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREENALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLGKQIESLSRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            2676..4331
                     /locus_tag="JFX52_RS27880"
                     /old_locus_tag="JFX52_27880"
     CDS             2676..4331
                     /locus_tag="JFX52_RS27880"
                     /old_locus_tag="JFX52_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_000012964.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSSAMESRKKKEDKKRSAENNLNDEKNKPRKGFK
                     DYGHDYHPAPKTENIKGLGDLKPGIPKTPKQNGGGKRKRWTGDKGRKIYEWDSQHGELE
                     GYRASDGQHLGSFDPKTGNQLKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4341..4598
                     /locus_tag="JFX52_RS27885"
                     /old_locus_tag="JFX52_27885"
     CDS             4341..4598
                     /locus_tag="JFX52_RS27885"
                     /old_locus_tag="JFX52_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523346.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_000523346.1"
                     /translation="MGLKLDLTWFDKSTEDFKGEEYSKDFGDDGSVMESLGVPFKDNVN
                     NGCFDVIAEWVPLLQPYFNHQIDISDNEYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     gene            4765..5022
                     /locus_tag="JFX52_RS27890"
                     /old_locus_tag="JFX52_27890"
     CDS             4765..5022
                     /locus_tag="JFX52_RS27890"
                     /old_locus_tag="JFX52_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_052904182.1"
                     /translation="MELKKSIGDYTETEFKKIIEDIINCEGDEKKQDDNLEYFINVTEH
                     PSGSDLIYYPEGNNDGSPEGVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            5084..5227
                     /locus_tag="JFX52_RS27895"
                     /old_locus_tag="JFX52_27895"
     CDS             5084..5227
                     /locus_tag="JFX52_RS27895"
                     /old_locus_tag="JFX52_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            5757..6671
                     /locus_tag="JFX52_RS27900"
                     /old_locus_tag="JFX52_27900"
     CDS             5757..6671
                     /locus_tag="JFX52_RS27900"
                     /old_locus_tag="JFX52_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277132.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNCYLFEKGRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVATSARTL
                     KPWEKLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(6888..>7118)
                     /locus_tag="JFX52_RS27905"
                     /old_locus_tag="JFX52_27905"
     CDS             complement(6888..>7118)
                     /locus_tag="JFX52_RS27905"
                     /old_locus_tag="JFX52_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_252356247.1"
                     /translation="LQRLKAKSRSLDTAQKVIVGAAMLARVRRPEEAQLRAFLLQFLRK
                     EVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="mobilization protein"
     BGC             1..7118
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(50..223)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          complement(224..582)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.389"
                     /coverage="100.0"
                     /evalue="3.99e-167"
     Typing          5758..6671
                     /name="rep_cluster_2134"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.891"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          225..2440
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.158"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctggcctca gcttcggcaa tcttctgttc gagtgttctg ctcatttcgt gactccgtgc
       61 gcggtgaaaa atcgcatttt agcgcgtcac tggtagttta aaaactaaac tggcataatg
      121 cacggcacat cacgaagtgc gcacttatac aatctccact ccgtttcgat tgtgtgcgcc
      181 ctgcggggct gacagaaaac ggcaaaaagg cattacggca gaaatggcga tttatcatct
      241 cagcatgaaa atcatttcgc gaaaaaacgg ctacagtgcc gttgcttctg ctgcctaccg
      301 ttccggctct gtcatacccg atgaccgtac cggattaacc cacgattaca cccgtaaacg
      361 cggcgttgat gatgcggtca ttctcacccc tgagaatgca ccgtcatggt gtgctgaccg
      421 ttccgttctc tggaatgcgg tcgagaaagc cgaacagcgc cgggactccc agctggcaag
      481 ggagattgaa cttgccattc cccgagagat ttcccgcgat gccgcacggg aaaccgttct
      541 cgcttttgtc agggaaaact ttgtcagcca gggcatgatt gccgatgtgg cgttccatca
      601 catggaccgg actaatcccc atgcgcacat catgctgacc acaagagctg tcggggaaac
      661 gggattcgca ggaaaggtca gggactggaa cgaccgggca ctcgccgaga cgtggcgcgc
      721 atcatgggct gaccatgcga acagagcgct tgctaacgcc ggctaccagg aagagataga
      781 ccatcgttca tacgagcgtc agggactgga gaaagcgccg ggccttcacc tcggaaagtc
      841 tgcctgtgcg atggaaaaac ggggcatgga aacagaacgc ggtgagcaga accgcctgat
      901 taacagcctt aacctggaaa tacaggtttc ccgcacgcag ctggcgctca ggaaggttca
      961 ggaaacgcag cgtaagcggg aactcagcga tgccgcacgt cgcgcagcgg aagcccttaa
     1021 tctgaccatt cccgccgcga atgcctcagc ggataccctg cgggaattca ttgccacact
     1081 gccgcaggaa tgcgggaacg catgggagat gacccctgag ttcgtggcga tgaacgggaa
     1141 ggcaaacgac atcaaacgtg aggagaatgc gctggtgaaa gagcgggata ttctcgaaaa
     1201 agagatgacc ggactcagaa aagcccgtcc ggtagcgtcc atcctgtcag agcttcccct
     1261 gatgacatgg gctgaaccgg aataccgcaa aagacaactc cgtttctgga aactcgggaa
     1321 acagattgaa tctctcagcc gcacttacag ggccgtgaaa gaacgggaca ttcccgcccg
     1381 tcgtcaggcc tttgaaacgc agtggaatac gtggattgcg ccgggaatgg cagagctgaa
     1441 agaaaaactg tcagcacggg aagcggagcg gcgcagggag gaagccgaag cggaagcgcg
     1501 ccggaaggaa caggagcatg aggcgcggct gaaacgtcat gataaccacc gtctgagccg
     1561 tgaaacggca ttagtcgggg ttattacgga gctggggcgt gccagagagc cgggaacggg
     1621 caggataacc cgctacatga tgttgagtaa cagagccgga gaattcacgg tatggggtga
     1681 tgagctggcg cattaccccc agagtgttca tgacccggtg aatgtttacc tgtcgccagg
     1741 cggggctgtg atggtctcgg atatacgtga gggaatgcca gaatctcatg agacgatggc
     1801 gcggcctgag cgtgtgagaa tgtattccgg tgcgacggtc cggcatgtac tggaacagat
     1861 gcgccagggg tggccctctt acggttttcc ggcgctgccg catcactggc cggataattt
     1921 ttatttcagc gacgaccgca ggcccgtagc ctctccgctg ccgtcagcgc accgggtgga
     1981 tatcaccgct gatgctgtac cggaacaact catgcctgtt gtattttcga gagagggaaa
     2041 cagcaggacg ctgaatctgt tgttatgcaa agggccggag gaaatgcttg tcgggtttgt
     2101 tcgccgggaa ggagggtttt gccccgttct tgtgcttccg tcgccggatt acagtcatct
     2161 gatagtcagc accatcacgg agaacggggt atgcctggca ggttacggag aagctataaa
     2221 ccatgatgag gatactccgt acccaccgga accgcacctg atgcagttcc ggctcaaagg
     2281 ccatcatgac aggcttttgg ctgctgtcca caaaccggaa gagatgccgg attatctctt
     2341 ccgtcaactc ggttttaatc agacctggca tgagtggaag cgggacgaac agcacaggca
     2401 acaacaacgc cgccccgggc acttccgggg catgagtatg tgatgtccgg ggctgcaccc
     2461 cggaccccgc caacacatca cgggccacaa aattttttgt ggcccgctct gcgttttcta
     2521 agtgttatcc ctcctgattt ctaaaaaatt ttccacctga gcttgacaga aaaaacgatg
     2581 atgggtactt tttgatctgt acataaaacc agtggtttta tgtacagtat taatcatgta
     2641 attaattgtt ttaacgctta aaagagggaa tttttatgag cggtggtgat ggacgcggcc
     2701 ataacacggg cgcgcatagc acaagtggta acattaatgg tggcccgacc gggcttggtg
     2761 taggtggtgg tgcttctgat ggttccgggt ggagttcgga aaataacccg tggggtggtg
     2821 gttccggtag cggcattcac tggggaggtg gttccggtca tggtaatggc ggggggaatg
     2881 gtaattccgg tggtggctcg ggaacaggcg gtaatctgtc agcagtagct gcgccagtgg
     2941 catttggttt tccggcactt tccactccag gagctggcgg tctggcggtc agtatttcag
     3001 cgggagcatt atcggcagct attgctgata ttatggctgc cctgaaagga ccgtttaaat
     3061 ttggtctttg gggggtggct ttatatggtg tattgccatc acaaatagcg aaagatgacc
     3121 ccaatatgat gtcaaagatt gtgacgtcat tacccgcaga tgatattact gaatcacctg
     3181 tcagttcatt acctctcgat aaggcaacag taaacgtaaa tgttcgtgtt gttgatgatg
     3241 taaaagacga acgacagaat atttcggttg tttcaggtgt tccgatgagt gttccggtgg
     3301 ttgatgcaaa acctaccgaa cgtccgggtg tttttacggc atcaattcca ggtgcacctg
     3361 ttctgaatat ttcagttaat aacagtacgc cagcagtaca gacattaagc ccaggtgtta
     3421 caaataatac tgataaggat gttcgcccgg caggatttac tcagggtggt aataccaggg
     3481 atgcagttat tcgattcccg aaggacagcg gtcataatgc cgtatatgtt tcagtgagtg
     3541 atgttcttag ccctgaccag gtaaaacaac gtcaggatga agaaaatcgc cgtcagcagg
     3601 aatgggatgc tacgcatccg gttgaagcgg ctgagcgaaa ttatgaacgc gcgcgtgcag
     3661 agctgaatca ggcaaatgaa gatgttgcca gaaatcagga gcgacaggct aaagctgttc
     3721 aggtttataa ttcgcgtaaa agcgaacttg atgcagcgaa taaaactctt gctgatgcaa
     3781 tagctgaaat aaaacaattt aatcgatttg cccatgaccc aatggctggc ggtcacagaa
     3841 tgtggcaaat ggccgggctt aaagcccagc gggcgcagac ggatgtaaat aataagcagg
     3901 ctgcatttga tgctgctgca aaagagaagt cagatgctga tgctgcattg agttctgcta
     3961 tggaaagcag gaagaagaaa gaagataaga aaaggagtgc tgaaaataat ttaaacgatg
     4021 aaaagaataa gcccagaaaa ggttttaaag attacgggca tgattatcat ccagctccga
     4081 aaactgagaa tattaaaggg cttggtgatc ttaagcctgg gataccaaaa acaccaaagc
     4141 agaatggtgg tggaaaacgc aagcgctgga ctggagataa agggcgtaag atttatgagt
     4201 gggattctca gcatggtgag cttgaggggt atcgtgccag tgatggtcag catcttggct
     4261 catttgaccc taaaacaggc aatcagttga aaggtccaga tccgaaacga aatatcaaga
     4321 aatatctttg agaggaagtt atgggactta aattggattt aacttggttt gataaaagta
     4381 cagaagattt taagggtgag gagtattcaa aagattttgg agatgacggt tcagttatgg
     4441 aaagtctagg tgtgcctttt aaggataatg ttaataacgg ttgctttgat gttatagctg
     4501 aatgggtacc tttgctacaa ccatacttta atcatcaaat tgatatttcc gataatgagt
     4561 attttgtttc gtttgattat cgtgatggtg attggtgatc aaatattatc agggatgagt
     4621 tgatatacgg gcttctagtg ttcatggatg aacgctggag cctccaaatg tagaaatgtt
     4681 atatttttta ttgggttctt ggttataatt gctccgcaat gatttaaata agcattattt
     4741 aaaacattct caggagaggt gaaggtggag ctaaaaaaaa gtattggtga ttacactgaa
     4801 accgaattca aaaaaattat tgaagacatc atcaattgtg aaggtgatga aaaaaaacag
     4861 gatgataacc tcgagtattt tataaatgtt actgagcatc ctagtggttc tgatctgatt
     4921 tattacccag aaggtaataa tgatggtagc cctgaaggtg ttattaaaga gattaaagaa
     4981 tggcgagccg ctaacggtaa gtcaggattt aaacagggct gaaatatgaa tgccggttgt
     5041 ttatggatga atggctggca ttctttcaca acaaggagtc gttatgaaaa aaataacagg
     5101 gattatttta ttgcttcttg cagtcattat tctgtctgca tgtcaggcaa actatatccg
     5161 ggatgttcag ggcgggaccg tatctccgtc atcaacagct gaagtgaccg gattagcaac
     5221 gcagtaaccc gaaatcctct ttgacaaaaa caaagcgtgt caggctgatt ctgatgcgct
     5281 ttttttttga aatgtcacaa aaattccatg tgggagatgg gatctaaaat cctcgtgcag
     5341 aactttccat ccagggggag aaaacttgtc gttttgagcc gttcggtgtt cagaacgcac
     5401 gaaaccgatc gcgcatcgct ttcgtgaata gttatgcagg cccctgaaaa cgattctgac
     5461 gagttttttc ggttttgcct ggtgttttcc tgtctttttg cgttttttgc gtcagaacgc
     5521 gtctgagggc gttttaaggg gtgcgtacaa cgggagttat ggtaaatgga tcggtttttc
     5581 gggaaggatc gacaggattt gccgttgggt gtagtgtaag cgactgaaaa acaaacgccc
     5641 cgtaaatcgt gctctcaccg ccaagattga tcacgaaatt acagggcgcc gggttccgcg
     5701 tttcccgatg ggaaagcgcg gttagttaaa ctgtgtaccg agagaaatcg tatcacatga
     5761 gcgccgtact tcaacgcttc agggaaaaat taccgcacaa accgtactgt acgaacgatt
     5821 tcgcgtacgg cgttcgcatt ctgccgaaaa acattgccat tcttgcccgt ttcatccagc
     5881 agaaccagcc acatgcactg tactggcttc cctttgacgt ggaccggacg ggggcatcaa
     5941 tcgactggag cgaccggaat tgtccggccc cgaacatcac cgtaaaaaat ccccgtaacg
     6001 ggcacgcgca tctgctctac gcgctcgccc ttcctgtgag aactgcgccg gatgcatcgg
     6061 cttcggcgct cagatacgct gccgctattg agcgtgcgtt gtgtgaaaaa ctgggcgcgg
     6121 atgtgaatta cagcggcctg atctgcaaaa atccgtgcca ccctgaatgg caggaagtgg
     6181 aatggcgcga ggaaccctac actctcgacg aactggctga ttatctcgat ttgagcgcct
     6241 cagcgcgccg tagcgtcgat aaaaattacg ggctggggcg aaactgctat ctgttcgaaa
     6301 agggccgtaa atgggcttac cgggctattc gtcagggctg gccggcattc tcacaatggc
     6361 ttgatgcggt gattcagcgc gtcgaaatgt acaacgcatc gctccccgtt ccgctttcac
     6421 ctcctgaatg tcgggctatt ggcaagagta ttgcgaaata cacgcacagg aacttcacgc
     6481 cggaaacttt cgcacagtat gtggctgata cgcacacgcc agaaattcag gctacacgcg
     6541 gtcgcaaggg cggttctaag tctaagcgcg gcacagtagc tacatcagca cgcacgctga
     6601 aaccttggga gaaattaggg atcagtcgcg cctggtatta ccaactgaaa aaacgaggtc
     6661 tcgtagagta gaccaaataa gcctatatca gataacagcg cccttttggc gtctttttga
     6721 gcagcttggt ttgttgctat ttccctcgtt gaatcccgca atggcgcggc tttccgcatg
     6781 attgaggtgg tagcgctcgc cgcagtctca tgaccgagcg tagcgagcga atgagcgagg
     6841 aagcgcaaag gcgtccggtg gtgcatgtgg cacttacgcg ccggggctta gtggttctgc
     6901 ggtttcgccg gtggtctggg tagcttctcc agctcgttaa tcagcggttg tagtcggttc
     6961 acatccgcct gtcttgtgac ttcctttcgc agaaactgga gcaggaacgc acgcagttgc
     7021 gcttcttccg gcctccgtac ccttgccagc atggctgccc ccacaatgac tttttgcgcc
     7081 gtgtccaggc ttcggctctt cgccttcagg cgctgtaa
//
