LOCUS       NZ_CP075704            11545 bp    DNA     linear   CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC2018-607 plasmid pSTEC2018_607-g.
ACCESSION   NZ_CP075704
VERSION     NZ_CP075704.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993387
            Assembly: GCF_016403625.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11545)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 11545)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: STEC2018-607
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:12:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,293
            CDSs (with protein)               :: 5,293
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 100 of 257
            Pseudo Genes (incomplete)         :: 139 of 257
            Pseudo Genes (internal stop)      :: 55 of 257
            Pseudo Genes (multiple problems)  :: 37 of 257
            Pseudo Genes (short protein)      :: 4 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075704.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC2018-607"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC2018_607-g"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2018"
                     /collected_by="ILS/NENT"
     gene            <1..183
                     /locus_tag="JFX52_RS27810"
                     /old_locus_tag="JFX52_27810"
     CDS             <1..183
                     /locus_tag="JFX52_RS27810"
                     /old_locus_tag="JFX52_27810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_215771238.1"
                     /translation="THPVPFFARFLLITGQMNPDCHVAKITLKRLRACSWADISTVMPI
                     CINPTGRSFLIQIKI"
                     /name="hypothetical protein"
     gene            193..387
                     /locus_tag="JFX52_RS27815"
                     /old_locus_tag="JFX52_27815"
     CDS             193..387
                     /locus_tag="JFX52_RS27815"
                     /old_locus_tag="JFX52_27815"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="WP_024194141.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWHDALHYAKG
                     ENIDWCIARAEYCKKTGRF"
                     /name="ANR family transcriptional regulator"
     gene            779..1006
                     /locus_tag="JFX52_RS27820"
                     /old_locus_tag="JFX52_27820"
     CDS             779..1006
                     /locus_tag="JFX52_RS27820"
                     /old_locus_tag="JFX52_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_062861059.1"
                     /translation="MSVDVIKQIQSIQDKGIIIYSKYRANEFDRDDVYRESYTLIIEFN
                     HLIAEHAVHDEKLINHTVCILNELRSIAIN"
                     /name="hypothetical protein"
     gene            complement(2062..3060)
                     /locus_tag="JFX52_RS27825"
                     /old_locus_tag="JFX52_27825"
     CDS             complement(2062..3060)
                     /locus_tag="JFX52_RS27825"
                     /old_locus_tag="JFX52_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_062861057.1"
                     /translation="MKNPELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGER
                     VGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPKIV
                     AEYPGARWLFLTLTVRNCSIDALGETLTAMNAGFLNLKRRKEFRGVLGWVRTTEVTRGK
                     DGSAHPHFHTLLMVSPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRKPKDD
                     ESLPCAVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGALKNVLQ
                     LERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
                     /name="protein rep"
     gene            4056..4250
                     /locus_tag="JFX52_RS27830"
                     /old_locus_tag="JFX52_27830"
     CDS             4056..4250
                     /locus_tag="JFX52_RS27830"
                     /old_locus_tag="JFX52_27830"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="WP_001367294.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAKG
                     ENIGWCIARAEYCKKTGRF"
                     /name="ANR family transcriptional regulator"
     gene            complement(6481..6903)
                     /locus_tag="JFX52_RS27835"
                     /old_locus_tag="JFX52_27835"
     CDS             complement(6481..6903)
                     /locus_tag="JFX52_RS27835"
                     /old_locus_tag="JFX52_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_032330703.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKSDTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7025..7303
                     /locus_tag="JFX52_RS27840"
                     /old_locus_tag="JFX52_27840"
     CDS             7025..7303
                     /locus_tag="JFX52_RS27840"
                     /old_locus_tag="JFX52_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032330704.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQINELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(7295..7585)
                     /locus_tag="JFX52_RS27845"
                     /old_locus_tag="JFX52_27845"
     CDS             complement(7295..7585)
                     /locus_tag="JFX52_RS27845"
                     /old_locus_tag="JFX52_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032330705.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEYDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            7826..9472
                     /gene="cka"
                     /locus_tag="JFX52_RS27850"
                     /old_locus_tag="JFX52_27850"
     CDS             7826..9472
                     /gene="cka"
                     /locus_tag="JFX52_RS27850"
                     /old_locus_tag="JFX52_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_165371048.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVVEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGTDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="cka"
     gene            complement(9469..9759)
                     /gene="cki"
                     /locus_tag="JFX52_RS27855"
                     /old_locus_tag="JFX52_27855"
     CDS             complement(9469..9759)
                     /gene="cki"
                     /locus_tag="JFX52_RS27855"
                     /old_locus_tag="JFX52_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_072149383.1"
                     /translation="MYLKYYLHNLPESLIPWILILIFNDNDNTPLLFVFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            9783..9914
                     /locus_tag="JFX52_RS27860"
                     /old_locus_tag="JFX52_27860"
     CDS             9783..9914
                     /locus_tag="JFX52_RS27860"
                     /old_locus_tag="JFX52_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001368419.1"
                     /translation="MKIVIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(10113..10256)
                     /locus_tag="JFX52_RS27865"
                     /old_locus_tag="JFX52_27865"
     CDS             complement(10113..10256)
                     /locus_tag="JFX52_RS27865"
                     /old_locus_tag="JFX52_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(10921..11118)
                     /locus_tag="JFX52_RS27870"
                     /old_locus_tag="JFX52_27870"
     CDS             complement(10921..11118)
                     /locus_tag="JFX52_RS27870"
                     /old_locus_tag="JFX52_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_028132815.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQTHFEAQYGIEQP"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1417..1494)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.25"
                     /coverage="87.0"
                     /evalue="2.63e-28"
     Typing          10753..10840
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.506"
                     /coverage="100.0"
                     /evalue="3.21e-32"
     Typing          2154..2343
                     /name="Col(MP18)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.476"
                     /coverage="99.0"
                     /evalue="1.71e-93"
     Typing          complement(10185..10742)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.405"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acacatcctg tcccgttttt cgcacggttt ttattgatta ccggacagat gaacccggat
       61 tgccatgttg cgaaaatcac gttaaaacga ttacgagcgt gcagttgggc tgatatatca
      121 acagtcatgc caatatgtat taacccgaca ggccgctctt ttttaattca aataaaaata
      181 taaattacga gtatgaatca ggacaaagca atttatttag aaaataaata tttttcaccc
      241 gcaaacgaag ctgtcagact ggagcgtgaa ggtgattaca ttggagcatg gaaaaaatgg
      301 catgatgcgc ttcattatgc caaaggcgaa aatatagact ggtgtatagc cagagctgaa
      361 tactgtaaaa agacaggccg cttttaattc tggcgggatt tcaaaggggc gtcccctttg
      421 gcacgtcgac gtatcaaaat gcgccagcat tttgtataca gtcggtaacg tgcctccaga
      481 atttcagcaa ccgcggactc ttaacaatca ataatcacta atacgcacta atacgcatta
      541 ataatcacta atgagcacta ataagcacta aatattgggt ctgcgccaaa cgaactcacc
      601 aacggatcgc atgtagtaga cgctacataa aaaatcctga aatggaggat tcgtcataaa
      661 gagattcctg tttttagcgt tctgatgcag gatagggcaa acaccgcaaa atgacgtctt
      721 tggtatgttt tgtgatgttt gcttctggca tctgttatct gttttattgg gtgtttttat
      781 gagtgttgat gtaattaaac aaattcagag catacaggac aaaggaatta ttatttattc
      841 aaaatacaga gcaaatgaat ttgaccggga tgatgtatac agggagagtt acaccctgat
      901 tattgagttt aatcatctta ttgctgaaca tgccgttcat gatgaaaaac tgattaatca
      961 tactgtctgt attctgaatg aattacgaag catagcgata aattaaaaaa tccccctgga
     1021 ttacaggggg aagccatcag agtttgagta atgaaaaata ttattttgga gagagcacca
     1081 caaatgcgct cttttccaat ctgccgtaac catcagcaaa agacaatcag gacaggcgaa
     1141 aaaatccccc tatatggcaa gggggtgtca atgaggacat ggcaattgtg agttttgtga
     1201 gttttgtgtg ttttatgtgt tgcagtttgc aacgtcagaa atttgccgta gttcgtcatg
     1261 agaggcaaca aaaaaatccc cccccggaac tggtggaggc cagggccaga ggggtagcaa
     1321 atggctaaat aattttcgta gttttgatat tcccatcgtt gcaaccgcaa cggggtgaat
     1381 ttgccgcagt tgtacgtgaa aaacaatcct gatttcggga caggatgtgt aatcgtataa
     1441 ccgcgcacgc acggatatac gattaccacc tggtcagggc tttgcccgac accctcgaca
     1501 cagttcccgc aatacgatag aacatcaggg cgcgtttccg ggtaaacgca tgttaatgac
     1561 cgggaattca ttgattaggg accggagacg cgctacaaaa ctcctgcgcg tttctggcat
     1621 caactgacca tggggatggt ttggcagtag cagcaagcgc cagaaacgcg ctcaaaatca
     1681 tcagatgtac agcgaacaaa aaattgtcat ctacaaaaaa tcacgttaaa acaatcgcga
     1741 gcaccttcct gtctccttca attacggtca aacattctgc atctgtcggg aagttgctca
     1801 cttccataat tcaatttctc attgacaata attccggtga tatcccctct tttgaggggc
     1861 ttttttttac ccacacacca caaacagcat ccatatcatc gtcaaaatcg cgctcaaaac
     1921 gtctctgacg gcctgaatca accaaaacat ataaaaccat tgccagatgc aaaaaatcgc
     1981 gttagaaagc gtctgagtgc gtttctggag agcaaaaagc tgtcagccgt ggatttccat
     2041 tgcagagacc cagaaacgcg ctcaatcatc gctgcttagc cgacgtttat ctttcgcccg
     2101 atcgcgctgg tattttttga caaccggcac ccacgaaaac gccagacggg aaccgtcatc
     2161 tgtaccctct gccatgccat ccgccatgat taaatcctcg ttcgtttccc gctccagttg
     2221 cagaacgttc ttaagcgctc cccccgtagc aacaaaacga cgcttgtgta cctgccgtgt
     2281 tagttcgagg aaccattcag gatcagccag catatcagca ggctttgtgg aatatttcag
     2341 agtctcaacc accgcgccgc gcagctgctc cgcaacagca caaggtaaag attcatcgtc
     2401 tttcggtttc cgtgacttca ccaccctgat atccacattc gggtcatacg ccacccgcag
     2461 acaatcacgc cacagttcca cccaacgggc atgtttcaca tactcacggc cggacaacat
     2521 cgagggggaa accatcagca gagtgtgaaa atggggatgt gcgctaccat ccttgccgcg
     2581 cgttacttcc gtggtacgaa cccagcccag cacaccacga aactccttgc gacgcttgag
     2641 attcaaaaaa cccgcattca tcgcggtcaa agtctcacca agcgcatcaa tggaacagtt
     2701 gcgcaccgtc agcgtgagaa acaaccagcg cgcgcctgga tattccgcca caatctttgg
     2761 cagggactga tagaaacggg cttgccacat catggagcgc ctccactggc agatcggaca
     2821 gtgccggaca cgacagaacc gcgcatcacg caaccgcaga cgcgtttctc cggtctctgt
     2881 gtttgttgac cacccaaacc gcaaaatacc actacaagcc ccgacacgtt cgccgtagtg
     2941 ctcaaatccg gcagcacgca gataaatacc gcccacatcg tcggaaaagc tccggcgctc
     3001 atcccacggt ttatcctccg gggaataatc cgtcagattt ttaagttcag gatttttcat
     3061 gtctgccgtt ccctagtgag cgacagacga aattacgtgt tatcattcct gtatcacctg
     3121 tgttgtcgtg tcaccaattc gaattcctac aggtggtcac acccccgaac gacgcccatc
     3181 gttgcggggg tcgcttttaa tgcgaccact tccgacaaaa tactctcaaa cctatttcca
     3241 cgcaaatcca cgttcaggcc agtcgtgaca aggcctagag catgatttcg agttgtcgat
     3301 ttatccctta agtatatata caaggtggcg accacctaaa aacgcatcaa tccacgagct
     3361 cccactggta gcacccagca cacgaaagaa catccgtaat aacaccactc ggcgtatgaa
     3421 tctcagccac aaccctttga ttaccgtagt aaggcgcata acccgaaagc ttcccgcgag
     3481 tcttcccacc aatggattta ataaccgcaa cagttctccc ccatgatgtc cgccgaacct
     3541 taaatccgac tctgttgcaa ttttcctcgt atagctgata gatatttttc aattcccatc
     3601 ctcctaaaaa cccgctttgc aggtggttgg cctcactgcg tgacaccaac caccatcacc
     3661 agtatttttt ctcgtaccgt cgtcaatcac aaaatcggaa cccacgccgg aggcgcggga
     3721 ccccacgatt ttctgtttga cgagtcagaa aacggcgaac gctgacagat ttcaatctgg
     3781 ctgcgtgaga cgttggcggg gtgtcggggc gcagtcctga ccaggcggca tttgtctgat
     3841 tgcgtgtgcg cggtccgaca aatacacatc ctgtcccgtt tttcgcacgg tttttattga
     3901 ttaccggaca gatgaacccg gattgccatg ttgcgaaaat cacgttaaaa cgattacgag
     3961 cgtgcagttg ggctgatata tcaacagtca tgccaatatg tattaacccg acaggccgct
     4021 cttttttaat tcaaataaaa atataaatta cgagtatgaa tcaggacaaa gcaatttatt
     4081 tagaaaataa atatttttca cccgcaaacg aagctgtcag actggagcgt gaaggtgatt
     4141 acattggagc atggaaaaaa tggaatgatg cgcttcatta tgccaaagga gaaaatatag
     4201 gttggtgtat agccagagct gaatactgta aaaagacagg ccgcttttaa ttctggcggg
     4261 gtttcaaagg ggcgtccact ttggcgcgtc gacgtatcaa aatgcgccag cattttgtat
     4321 acagtcggtc acgtgcctcc agaatttacc ctcaaaacgc ccctgcaact gatacacaac
     4381 aaaagtataa ctaatatgta gttattatat aactaatgca taataaacag ctcaccaacg
     4441 gatcgcatgt agtagacgct acataaaaaa tcctgaaatg gaggatttgt cataaagaga
     4501 ttcctgtttt tttagcgttc tgatgcagga cagggcaaac accgcaaaaa tgatgaaaaa
     4561 ctgattaacc atattgtctg tattctgaat gaattacgaa gcatagcgat aaattaaaaa
     4621 atccccctgg attacagggg gaagccatca gagtttgagt aatgagaaat attattttgg
     4681 agagagcacc acaaatgcgc tcttttccaa tctgccgtaa ccatcggcaa aagacaatca
     4741 ggacaggcga aaaaatcccc ctatatggca agggggtgtc aatgaggaca tggcaattgt
     4801 gagttttgtg tgtgttttat gtgttgcagt ttgcaacgtc agaaatttgc cgtagttcgt
     4861 catgagagac aacaaaaaaa tcccccgtga ctggtggagg ccagggcaag gggggtagca
     4921 aatgcctgaa taattttcgt agttttgata tacccatcgt tgcaaccgca acgggatgaa
     4981 tttgccgcag ttgtgcgcga aaaacaatcc tgattccggg acaggatgtg taatcgtata
     5041 accgcgcacg cacggctatg cgattaccac ctggtcaggg ctttgccccg acagcctcga
     5101 caccgttccc gcgatacgat agaacatcag ggcgcgtttc cgggtaaacg catgttaatg
     5161 accgggaatt cattgatcag ggaccggaga cgcgctacaa aactcctgcg cgtttctggc
     5221 atcaactggc catggggatg gtttggcagt agcagcaagc gccagaaacg cgctcaaaac
     5281 gtctctaaca gcctgaaccc accaaaacat aatgaaacat tgccagatgc aaaaaagtgc
     5341 gttagaaagc gtctgagcgc gtttccttgc cacctgaacg tcatctctgg gattcgagct
     5401 cactccatca aataacaaat aaacttcaaa gtctgataat gggttccccc tgtaatccag
     5461 gggatttttt tcgctcactc attaccgcac aactcgacat cacgccggag acacgctaaa
     5521 acaccctgcg cgtttctggt gcaaagagta gttcgtaggg atgattacag tcaggtatgt
     5581 accagaatcg cgccctgaac acggctgaac gcctcaaagc gaaaccattg cctcatgtaa
     5641 aaaatcgcgt tacaaagcct ctgagcgcgt ttctggcgtc ttttttgttg ctgtgtcgct
     5701 atactcgttg tcagattcgc gctcaatcat cactgattaa tctgcgctta tccttcgacc
     5761 gatcacgctg gtattttttg acaaccggcg cccatgaaaa cgccagacgg gaaccgtcat
     5821 ccgcaccctc tgacatgctg gggattaaaa atccctgtgc cgcacagccg gcgttgcata
     5881 aaattccgct ctgcgatgag tattgtttaa tcaaaaatcc ccgcagtttc tccagaaaaa
     5941 cgcgagtctg ttctcatgcc ccggatgccc accccccacg caaaaccaac ttttttctga
     6001 tttgcttttt aaatcatgtt gttatggttt gaaaaatata ttttcttatt cagatttata
     6061 caaaagcgag ttgtcggcga ctactggatg attcggcaac gagtcgtcgg tgatttatcg
     6121 atgactcaac acactgtctc tctggtgttc agaaaaagcg agtcgccaga gagtcgcgag
     6181 gaaaaatact ggatgactcg ctgatgactc ccggggtgaa tcgctcacga ctcgtctgta
     6241 actactgtct gatttcttct catcagggct gaaaacgtcc gaaatgcgcc ttatgggcat
     6301 caggcgcgcc caaaccgatc gccattcagt ttcatgcata gtcatgcagt gccatgaaaa
     6361 caatcctgac gtatttttcc ggtttacccg ggggaaaaca tctctttttg cggtttttgc
     6421 gccagaatcg cgctgagcgc gtttcagcgg tgcgtacaat aagggattat ggtaaatccc
     6481 ttactgtctg ccctcgtatc catcgagata aaccgcagaa atggtgtctg ccattagttt
     6541 catttttttc ggcgaaggca tatcgctgtt tgtgtatttt ctgccagcaa aggttgttgt
     6601 gctccggagt gagtgaaaaa atttttctga gtggaattct tttacacgtt gctgtgccgt
     6661 ttctgctgat actccggctg ctttgtcttt catccccata tcccggaact gctgaaagaa
     6721 agggagcatc atacgtatgg tgtctgattt ctgggtgtag aagttaggat caaatccatc
     6781 atctgaagca taaacataat gccgggtatg tcgttccagg ctggtctttg gtgtgcatcc
     6841 tgtaagagct attgttgctg ttatcagagg tatcagaagc agcctggcag cggcagatct
     6901 cataaacatc tccttgcgtt tttaatcttg atactcaggg agttatatct ccgaagagta
     6961 tctcttatca tacgtcggtt tactcccaat gtgatttgat ggtcaatgag tcaggaataa
     7021 tcccgtgaaa aaatgtgtgc cagtcctgct ttttagtgtt ctttctgctt cggttgccag
     7081 tcatgcggac agcaatgttt ttcaacaaaa ggccgagaag acaaaaaacg gcattgttgc
     7141 ggatgttaat gccagacaga aaatgttcag taacagactg aagatacaac ttcagatcaa
     7201 tgagttgaga gcagagctgg ctggcaccag agatgcccaa aaaagagagg atttgcgtaa
     7261 aaaactggag tctctggagc ggcaaaagaa atccttacgc tgatgggggc gtattttccc
     7321 cggtttcttc cataaccttt ttaccgttca gatatttacg ggcgaggcga atgcgtcttg
     7381 cctggtatac cgctgagtaa gcctgggtgg gattcacacc attactgatt tcactgacca
     7441 catgacgctg atcagccagt gtcagcgtgt aaacctgttt ctggatgagg tcacgggctt
     7501 tgtcatactc atatccggca gcaacaaggg tgtcgagaag tcgtttgtct gttttgttca
     7561 gtttatggcg gggtgatttg ctcacatcgt tctccgtttt ttcatgatcc aacagtttta
     7621 tatctcatcc tgatttgctc cggtgttctg tttcgacgtt ctcaatgcct gcctccccat
     7681 agtgtgatcc ttattgcaaa aatgaaaatc catgctcttg acacggacaa tgctgagtag
     7741 taggttttca ctgtacataa aaccagtggt tatatataca gtatattgtt tttaatttat
     7801 tgttttaatt atcaaagagg aatttatggc taaagaacta agtggatatg gcccaactgc
     7861 tggtgagtct atggggggaa caggggcgaa tctgaatcag caaggtggaa ataataatag
     7921 taatagtggg gttcactggg gtggtggctc tgggcatggt aataatggtg gtcagggaaa
     7981 ttcaaattca tctggttcta catcaactgt gatgaaaacc ggtgagtcat atctgactcc
     8041 gtggggtgat gttgtcatta ataatgatgg ccttccggtg atgaatggta ttgttatgac
     8101 agaagagaat tcaactctgg ttgataatcc atttggaggg gtctccagag ttcttaattc
     8161 tttaattagt gatatgccat cactttttgc agaaagctca ggaaataaca acaataacac
     8221 tgcttcagtt aatactgcac caacgaatgc tcaggtcagt gatatggata agagttcgaa
     8281 ggtggtgggt aatgtaatta atgaaaaaca aaagcagaag aataaaattg ctacacagat
     8341 tagtgaaaaa cagaagaaaa tcgaggagat gaaaaaagta ttcaagcatc attcttatca
     8401 tggtatcact gatcttgaga gggatgttga tgagttgcaa aaaaaatcaa atcagcttga
     8461 tgcagatatt agcaaactta attcttataa aaatacattg caaagtaaaa ttggtgatgt
     8521 gaataaacag aaggaagctg aagaaaaggc tcgtgaaaat gcagaggtcg ctgaacatga
     8581 aactcttaat gaagagaaac aggcggtggt ggaagcagag aagcgtttag ctgaggcgaa
     8641 agctgaactg gcgaaggctg aaagtgatgt acagagtaag caagctactg tttccagagt
     8701 tgcaggagag cttgaaaacg ctcagaagag tgttgacgtg aaggtcaccg gatttccggg
     8761 atggcgtgac gttcagaaaa aactacagcg tcagcttgaa gctaagcagg ctgagtactc
     8821 cgcagttgaa aatgaactta agaatgctgt tagtttcagg gatggtaagg ccgctgaggt
     8881 taaagaggca gagcagaaat taaaagaagc tcaggatgct cttgaaaaga gccagataaa
     8941 agatgctgtt gatactatgg ttggatttta tcaatatata accgaacaat atggagaaaa
     9001 atacgcaaaa attgctcagg atttagcgga aaagtctaaa ggaaagaaaa ttcaaggtgt
     9061 tgatgaagct ctggcagcat ttgaaaagta taaaaatgta ctggataaga aattcagtaa
     9121 ggttgatcgg gatgctattt ttaatgcgtt agagtctgtt aattatgatg agttatctaa
     9181 gaatttaacg aagatatcaa agtcgcttaa aatcactagt cgagtatctt ttctatatga
     9241 tgttggtact gattttaaaa atgcaataga aactggtaac tggcgacctt tgtttgtcac
     9301 gctggagaaa agtgcggtag atgtaggtgt ggcaaaaatt gtggcattaa tgtttagttt
     9361 tattgttggt gtgcctcttg gtttctgggg gattgcaatt gtcacaggta ttgtttcttc
     9421 ttacataggg gatgatgagt tgaacaaact taatgagttg ttagggattt aatttctctt
     9481 tagagtgtaa tatatgaaac ttggcaatcc tactgggatt gccattaata aataaaacaa
     9541 tgcagataac ttgcataagt accagggctc tttttttaac tttgtatttt ctttgatata
     9601 tctagatatg gttaatttag agtatggata tagcaataca tgtattgatg atataaagac
     9661 aaataacaaa ggagtgttat cattgtcgtt aaatattaaa ataagaatcc atggtataag
     9721 tgattcaggt aaattatgta ggtagtattt tagatacata atttttcttt tgttggaggt
     9781 gtatgaaaat agtaattttt ctaattacaa tattattctt gtcaggatgt caagcaaact
     9841 atattcgtga tgtacaagga ggaaccgttg caccatcttc gtcttctgaa ctgacgggga
     9901 tcgcggttca gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     9961 ctgctgcttg caaacaaaaa aaccaccgct gccaacggtg gtttgtttgc cggatcaaga
    10021 gctaccaact ctttttccga aggtaactgg ctttagtgga gcacagatac caaatgctgt
    10081 ccttccagtg tagccgctat aaggcttcaa cttcaagagt tctgcgtatc taaatccact
    10141 atccagttac caatggctgc tgccagtggc gttaagtcgt gtcttaccgg gttggactca
    10201 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10261 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
    10321 agcgccacgt ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10381 acaggagagc gcacgaggga gcttccgggg ggaaacgcct ggtatcttta tagtcctgtc
    10441 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10501 ctatggaaaa acgccagtgg cgcggccttt tccgggttct cgttttttgc gcacatgttt
    10561 ctttcggcgt tatcccctga ttctgtggat aacggtatca ccgtctttga gtgaggtgac
    10621 tccgctcgcc gcagccgaac gaccgagcgt agcgagtcag tgagcgagga agcggaagtg
    10681 taccggaagc tgcattttct ccttacgcat ctatgcggca tttcacaccc ggcatggtgc
    10741 acttttcata caatccgcac tgatgccaca tgattaagcc agtatacact ccgctaccgc
    10801 tacgtgactg ggtcatggct gcgccccgac gcccgctaaa gccccctgac gtgccctgac
    10861 gggctttgtc tgctggcgaa aacactcacg gacaagccgc gaccactttc gccagcgcct
    10921 ttatggttgt tctatgccgt actgagcttc aaagtgagtc tgtatgctgt tctggagttc
    10981 ttccgccagt tcgtgcagtc gttcgcacat ggcggcctgt tcgtctgcat ccagtgcatc
    11041 cagtttttcg agcaacgtca ggctctggct tttgataaat cgcgccatgt tgagcgcggt
    11101 ctgctgctgt ttgttcacgt tctcccgtct cctttactgg tgtttacgtc cggcagtgcg
    11161 tcacctggtg tgatgacccg gtgacgtcac tgaaaattat cacacaagcc cagtcgcgcg
    11221 tgtgctgcgc gacattaaca ccccgtgacg cttatttttt gctgaatatg tgcgcgattt
    11281 tctgtctgcg ttgtgtgcag ttgttgtcat aaacacgaat gttccggctg ttcctgatgt
    11341 gcgtcacttt gacgttttga caaagtgacg cacgggaggt gcttgcgcaa acccgcgaat
    11401 aacggcgttt accgcgtgat tttttaacat ctgacaaagt gacccggcgt gatggccagt
    11461 tctggccaga gacgttggcg gggtgtcggg gcgcagccat gactaggcgg catttgtctg
    11521 attgcgtgtg cgcggtccga caaat
//
