LOCUS       NZ_CP075703            24412 bp    DNA     linear   CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC2018-607 plasmid pSTEC2018_607-f.
ACCESSION   NZ_CP075703
VERSION     NZ_CP075703.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993387
            Assembly: GCF_016403625.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24412)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24412)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 24412)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: STEC2018-607
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:12:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,550
            Genes (coding)                    :: 5,293
            CDSs (with protein)               :: 5,293
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 100 of 257
            Pseudo Genes (incomplete)         :: 139 of 257
            Pseudo Genes (internal stop)      :: 55 of 257
            Pseudo Genes (multiple problems)  :: 37 of 257
            Pseudo Genes (short protein)      :: 4 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075703.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..24412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC2018-607"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC2018_607-f"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2018"
                     /collected_by="ILS/NENT"
     gene            <1..287
                     /locus_tag="JFX52_RS27630"
                     /old_locus_tag="JFX52_27630"
     CDS             <1..287
                     /locus_tag="JFX52_RS27630"
                     /old_locus_tag="JFX52_27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252356246.1"
                     /translation="YIESTTQQIFIFNTLTLCTIGLIHIETGEKYIVVFTDNNKIRDYK
                     NGVIEELGEITQEDIELIEYYAKKYEKYFDDLKIGEEPLNLKEYIECMQ"
                     /name="hypothetical protein"
     gene            658..1191
                     /locus_tag="JFX52_RS27635"
                     /old_locus_tag="JFX52_27635"
     CDS             658..1191
                     /locus_tag="JFX52_RS27635"
                     /old_locus_tag="JFX52_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025389.1"
                     /translation="MNIQEALNIFGLSGQLTEKDIKNAFKKAALKYHPDRNPVGAEMMK
                     AVNSAFDFLMANLDKINQYQSPEQSAIYNYGDELENVLKALYGLSGLVYEVIGNWIWIS
                     GNTIEHKEALKEIGCKWAAKKKQWFYRPEEHKSYWNREEHTIEEIRAKYGTSGKFQAGG
                     WQQVEGRARVEARA"
                     /name="J domain-containing protein"
     gene            1319..1861
                     /locus_tag="JFX52_RS27640"
                     /old_locus_tag="JFX52_27640"
     CDS             1319..1861
                     /locus_tag="JFX52_RS27640"
                     /old_locus_tag="JFX52_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000206647.1"
                     /translation="MTTFSEKYQPVFEVVCRILGNGWRVNKLDNYQHRIKLTSTEIRKY
                     SIIIRLERERFFIVGSVDSLVWHGNPSVCTVSLTRNPVGIANDISRKILANALDEIELL
                     REHESALQEKQDKVRIIKGMLSQLLSISPWYGALTGFNADNGLAGKILQNGDDYEMVIR
                     GLNVDQLIKVAGFIKKL"
                     /name="hypothetical protein"
     gene            1897..2154
                     /locus_tag="JFX52_RS27645"
                     /old_locus_tag="JFX52_27645"
     CDS             1897..2154
                     /locus_tag="JFX52_RS27645"
                     /old_locus_tag="JFX52_27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000845367.1"
                     /translation="MKTDNVFFKSKGSYSYVVTVDMDNQMYMVRKYVKTYVKSCDKEQV
                     KGKKTVKKLVLDTNLTLPELLDLDNGILADLFTRGNNNAN"
                     /name="hypothetical protein"
     gene            2144..2389
                     /locus_tag="JFX52_RS27650"
                     /old_locus_tag="JFX52_27650"
     CDS             2144..2389
                     /locus_tag="JFX52_RS27650"
                     /old_locus_tag="JFX52_27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001193619.1"
                     /translation="MQTKPTPADVKEARISAGLTLKEAAEIFGYQLNTWQMKESAGKAT
                     RSLSVGEYNYLLLLAGKHPEYVLCKRDTITAEPTTV"
                     /name="hypothetical protein"
     gene            complement(2420..2719)
                     /locus_tag="JFX52_RS27655"
                     /old_locus_tag="JFX52_27655"
     CDS             complement(2420..2719)
                     /locus_tag="JFX52_RS27655"
                     /old_locus_tag="JFX52_27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001259443.1"
                     /translation="MRTLEEVIAAQSPESQAIIKAMADEMILETGLQMMREELQLSQKQ
                     VADAMGISQPAVTQLEQRGNDLKLATLKRYVEAMGGKLSLDVELPTGKRVAFNI"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2716..3066)
                     /locus_tag="JFX52_RS27660"
                     /old_locus_tag="JFX52_27660"
     CDS             complement(2716..3066)
                     /locus_tag="JFX52_RS27660"
                     /old_locus_tag="JFX52_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269926.1"
                     /translation="MWTVYFGRLFDEWFEEQELALKRKVLAELKHLEEFGPSLSRPHAD
                     TVKGSRHKNMKELRIQYEGHPIRAFFAFDPIRQAIILCAGDKSNDKKFYDRMIRIADEE
                     FSTHLAELEGKK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3432..4079
                     /locus_tag="JFX52_RS27665"
                     /old_locus_tag="JFX52_27665"
     CDS             3432..4079
                     /locus_tag="JFX52_RS27665"
                     /old_locus_tag="JFX52_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214917.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000214917.1"
                     /translation="MTVAIALVNGKGGVKKSSMSRAIATEYTKADWKVLGLDMDVDQGS
                     FLDWGQRRLRYKVEPPVTIQGCGTVSQAKKHIESGIWDLVVIDSAAYATKSLLSLAEIV
                     DLLVLPSGFSVDDLRTTVTTVNALRNKGHSTNKMAVVFVGVSESESEYRTALEYLKPCG
                     VQVINGAIPFMTSFSQAQDKGLALTESPFPGPRQKADEVVQGVIDLLTKLSQ"
                     /name="ParA family protein"
     gene            4119..4352
                     /locus_tag="JFX52_RS27670"
                     /old_locus_tag="JFX52_27670"
     CDS             4119..4352
                     /locus_tag="JFX52_RS27670"
                     /old_locus_tag="JFX52_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000024515.1"
                     /translation="MANVPPKPRNTKGEPPSLEDTQHGFNPTRKNADGRKAQLNLYVDM
                     EVKREFRSIAAANDMKHGELLEAYQEFWKKHH"
                     /name="hypothetical protein"
     gene            complement(4384..4647)
                     /locus_tag="JFX52_RS27675"
                     /old_locus_tag="JFX52_27675"
     CDS             complement(4384..4647)
                     /locus_tag="JFX52_RS27675"
                     /old_locus_tag="JFX52_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="WP_001312455.1"
                     /translation="MTIKILVVSNDGHEKLIDLSPDNDLAKITKSLRTAENRMVCVIQD
                     NNRILRWDRSYASRAKNHWRKVAPDRFEILGTVEHIHYVGKC"
                     /name="PilI type IV pilus biogenesis protein"
     gene            complement(<4826..5131)
                     /locus_tag="JFX52_RS28490"
                     /pseudo=""
     CDS             complement(<4826..5131)
                     /locus_tag="JFX52_RS28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5141..5605)
                     /locus_tag="JFX52_RS27685"
                     /old_locus_tag="JFX52_27685"
     CDS             complement(5141..5605)
                     /locus_tag="JFX52_RS27685"
                     /old_locus_tag="JFX52_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_000697615.1"
                     /translation="MKILIIFALYILSPSLYANAADIQGKVIRVLDGDTIEILQNKTPV
                     RIRLANIDAPEKKQAYGRWSTDQLKSLIAGQSVRVTYSHNDRYGRIIGRVFTTGGSEAN
                     RFMVQSGAAWVYEHYNTDNALADLQQEARSQKRGLWADPNPVPPWEWRHK"
                     /name="thermonuclease family protein"
     gene            complement(5609..6343)
                     /gene="mobC"
                     /locus_tag="JFX52_RS27690"
                     /old_locus_tag="JFX52_27690"
     CDS             complement(5609..6343)
                     /gene="mobC"
                     /locus_tag="JFX52_RS27690"
                     /old_locus_tag="JFX52_27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_000905147.1"
                     /translation="MLIHDFYERKRISANNMLCLLNFLKDETYSDFHIIKRKLGFTKKS
                     RNPVYKLLNKALKEGFIIKHEIPSSKRKLTVWGITMQGLAMVVKADDITFPSYFEPSKL
                     KYWTLEHRLLNQRVRISLESKGATEWINGDSAAFIARYSGCSHRPDGVIKQPTGAIMAI
                     ETERTMKTRARYISIINSHLTARDKGNWHYALYVMPDTKSKESLIRLFDSIKTVMRNNT
                     PVPFDAKNREMFIFRTIDELEQ"
                     /name="mobC"
     gene            complement(6353..8080)
                     /locus_tag="JFX52_RS27695"
                     /old_locus_tag="JFX52_27695"
     CDS             complement(6353..8080)
                     /locus_tag="JFX52_RS27695"
                     /old_locus_tag="JFX52_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615560.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_001068080.1"
                     /translation="MNPHLMQKLNGIADAFSIFLLWLQNSPVILSLLAGLTLPFIFNLP
                     REERKNAPFWLKSVACVSIFFFIFGTMSPLSIHGLSYLFDVLDPNTLLRIPLWIITILF
                     TLAGLAFHLTARRLLAGEIDSLKHRMIKKSKLERNTRTDVRKVKELLPDSIEYNPLDYI
                     DLKKGVFIGLDKDDQPQYITIKEFQKQHAAIIGTTGSGKGITASVLLYQAILLGEAVFV
                     EDPKDDEWAVHVLREACKKAGKKFVVINLNRTNHQLDLLADISHEQLEELFNAGFSLSK
                     KGEAADFYRIADRRAARNVAAIHKTGMTLYDLFNDDNVKSIGEDAPAFLGELEEIALVN
                     SVNAKNGFSLKEIFDNGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINTTPRVV
                     AIFLDELKYHISRPTMEGLGTARDKSVHIFMAFQAIDDLRDCPSDLNGDSVIGAVIENA
                     KFKLIYKIQNPETAEWAAKMTGSILVDDEMRKVRTDLSLTEKVDTDRMIRQAESYYVDS
                     NMFLNLPERVGFVFTSKDLPKATKMSHIPANKETIELLSFETEQIEQHNNNDVPSSGL"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            complement(8890..9195)
                     /locus_tag="JFX52_RS27700"
                     /old_locus_tag="JFX52_27700"
     CDS             complement(8890..9195)
                     /locus_tag="JFX52_RS27700"
                     /old_locus_tag="JFX52_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000910475.1"
                     /translation="MLKEDMFINNIHSRNQDRIHVKRVFDDISNKANRGCGLYYEIYQA
                     RLMRGLIEHTVKLKSSDANKLISYAASQGYEITEDRYNAAIKAEDECLTEITRAQV"
                     /name="hypothetical protein"
     gene            complement(9223..9438)
                     /locus_tag="JFX52_RS27705"
                     /old_locus_tag="JFX52_27705"
     CDS             complement(9223..9438)
                     /locus_tag="JFX52_RS27705"
                     /old_locus_tag="JFX52_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001090643.1"
                     /translation="MNTDENMVFYLTNSDLVYSYAHKIVCSIPSGMKGNFITSIGETLE
                     DLKKRYPDIILITESEALALQSKREN"
                     /name="hypothetical protein"
     gene            complement(9441..9740)
                     /locus_tag="JFX52_RS27710"
                     /old_locus_tag="JFX52_27710"
     CDS             complement(9441..9740)
                     /locus_tag="JFX52_RS27710"
                     /old_locus_tag="JFX52_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000716758.1"
                     /translation="MKKIAAITMFVLTTATPVYSYAADPCEVVLCMYGKATGNSGGGEC
                     RSAEKAFFNILKKKKGSIRWSKTFDARKAFLNQCSSADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            complement(9769..10167)
                     /locus_tag="JFX52_RS27715"
                     /old_locus_tag="JFX52_27715"
     CDS             complement(9769..10167)
                     /locus_tag="JFX52_RS27715"
                     /old_locus_tag="JFX52_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_021533590.1"
                     /translation="MIKHILVTVLVTLITGCSSPPPPVPVEWNKKPVSLNEKLPEWNPN
                     SLIIKSPVINDKWTLSISSQDFNKEDWMPSVFYAIAHSTQITVATKSGADYFSAKNWLY
                     HHGANGVIIYHPITNCLTCNETTIYFSR"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            complement(10164..11189)
                     /gene="virB11"
                     /locus_tag="JFX52_RS27720"
                     /old_locus_tag="JFX52_27720"
     CDS             complement(10164..11189)
                     /gene="virB11"
                     /locus_tag="JFX52_RS27720"
                     /old_locus_tag="JFX52_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125556.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000125556.1"
                     /translation="MTAKNISLDRYKQRFFGDFLELPGLTEIAVNRPGELWTKIDGVWG
                     QHTVDLKFDDCYNFARCLAKHHNDNIEDIKPGLSATLESGERCQVVVPPACERDTISIT
                     IRKPSKIQIPHQAYIDSGFYNRVTGIEKASTHNEELIELYKSKNIPLFMEKCVEYGKTL
                     AVAGETSTGKTTYMKMLIQYIPVHLRITTIEDNPEIIFYKHKNYVHLFYPSESSDEKGS
                     VVTPAALIRWNYRMNPDRILLTEVRGGEAWDFLKITGSGHEGSMTSIHAGSAHEAIDGF
                     ITRCTENPQCAQLPYSFLLRKVLDSLDVVVSIDLDGNVRRMNDIYFRPLHRNQYYEEMK
                     V"
                     /name="virB11"
     gene            complement(11179..11370)
                     /locus_tag="JFX52_RS27725"
                     /old_locus_tag="JFX52_27725"
     CDS             complement(11179..11370)
                     /locus_tag="JFX52_RS27725"
                     /old_locus_tag="JFX52_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000995652.1"
                     /translation="MNCKDKETWLHMLAAGHMGGLAGNPNEAAAQDAYQRLIKNKTAQA
                     VLQRCTTRITLKGANDDR"
                     /name="hypothetical protein"
     gene            complement(11354..12619)
                     /locus_tag="JFX52_RS27730"
                     /old_locus_tag="JFX52_27730"
     CDS             complement(11354..12619)
                     /locus_tag="JFX52_RS27730"
                     /old_locus_tag="JFX52_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="WP_198777268.1"
                     /translation="MTDENKSPSTEEEKPVMTSDQDDIATLEREARAKREAELQASQDD
                     EKENSLQPAVNKLKKRQKGKAAAFLAICAVALIGLTWGGNWVYQNFLKQENGEKTKEVT
                     PPKKTNDYRKRTDLGMATNEPENSTEGEQAVSQDNTGNVSGTAGQMKTFEPPQLNKASF
                     LVRRNNTTSPQNQVKTRQQEMILAAENNATQPTDSTATTTQHGTASQAISPATVRRIPY
                     NPDLYIPENTVIPCSMDYRFVSDRAGKIRCTVAKDIWSASGNTKLIEKGTTATGIYQTG
                     ITQGQGRAFIMMTKLRTRQRPYLDIPLIDTNAAGELGESGVDGWIDTHFWERFGGALMV
                     GMIPDIGAWASNNAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKNQGEIINL
                     ITGQDIDFSNIYTLRLKNELQR"
                     /name="TrbI/VirB10 family protein"
     gene            complement(12661..13566)
                     /gene="virB9"
                     /locus_tag="JFX52_RS27735"
                     /old_locus_tag="JFX52_27735"
     CDS             complement(12661..13566)
                     /gene="virB9"
                     /locus_tag="JFX52_RS27735"
                     /old_locus_tag="JFX52_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001230135.1"
                     /translation="MRKLTLITLIIMLNSQAWAAATPQASRYDARIQQVIYNPQNVTVV
                     NTKPGFMTTLVFDNDEAVISASPGFDEAWEARADANRVNIRPVALTQGAPGEDGNTTQV
                     VIPPNSRDWHTNMLIVTNKRLYNVELNVIDEKSPQQPAYLVSYRYPGDERNRANKEAAA
                     RQKEWEQKQEQTQIQKALRTAQTPRNWDYTKYPGKDSYNIEPDFAYDDGRFTFLGFSPN
                     KSVPSVTKEQNGQEYVVNSSIQRKGNFTVLVIQEITPRLVLRSGDAVVGLKNNGFGKVH
                     AADNSTVSDKVERVEKTDNT"
                     /name="virB9"
     gene            complement(13566..14249)
                     /locus_tag="JFX52_RS27740"
                     /old_locus_tag="JFX52_27740"
     CDS             complement(13566..14249)
                     /locus_tag="JFX52_RS27740"
                     /old_locus_tag="JFX52_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000139290.1"
                     /translation="MTEQKLIADSRSFEQQMIDRDKRATKAGFLVGGAGLLITILSLIA
                     IVIMLPLKQTTVELYTVDNHTGRVEHVTRASKTALTAEEAYQKAMAANYVKLRERYVYP
                     ALQDDYETVQIYNSPQVNDDYLALYAGKNAPDKVYNNAAHTVKIEIISEQITNATAPDK
                     VATVRYKKIIRRMADNSIRNEYWDARFTFHSDPEKEMSDVEREINPFGFTVTSWQTDRE
                     IRGGE"
                     /name="type IV secretion system protein"
     gene            complement(14242..14385)
                     /locus_tag="JFX52_RS27745"
                     /old_locus_tag="JFX52_27745"
     CDS             complement(14242..14385)
                     /locus_tag="JFX52_RS27745"
                     /old_locus_tag="JFX52_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000825480.1"
                     /translation="MKRLIALALLCVLSGCAQKSKSLPHAFSGQGSPVNTPQIIAELTT
                     HD"
                     /name="hypothetical protein"
     gene            complement(14435..15505)
                     /locus_tag="JFX52_RS27750"
                     /old_locus_tag="JFX52_27750"
     CDS             complement(14435..15505)
                     /locus_tag="JFX52_RS27750"
                     /old_locus_tag="JFX52_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_198777269.1"
                     /translation="MMAGIFSSVEKNIIGGITDATNGLMMSYSSMMMGLAATSATIYIM
                     WRGYQTLAGKLSTPMEDVMWDIMRMAIILSFVANINGYLDGVIDAVNGIKEGFSSSTSI
                     WQLLDTLWNSARVLGKTLHDLDNSEFIKEEGTIAELFVWGGVFVLMIITAFVSMIAEIM
                     ILLLGITAPIFIFCLMYGFLQPMFNNWLQNIFAAILTIMFSALALRIVIGFLTSRMSEA
                     VTQASKANIVELGAQVCLAGVCAAILVYMSAKLAGALAGASATATMQGAAALGIGAAAY
                     GAGKMLKEAGKVLPKEVASPSNKGLGDPQSRIVFNAADARKHAIEQMIARNEARMLTDQ
                     SAPKLPPSIKRLPPPR"
                     /name="type IV secretion system protein"
     gene            complement(15515..15742)
                     /locus_tag="JFX52_RS27755"
                     /old_locus_tag="JFX52_27755"
     CDS             complement(15515..15742)
                     /locus_tag="JFX52_RS27755"
                     /old_locus_tag="JFX52_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010617327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000720509.1"
                     /translation="MKKIILFTIISGLMALTSGCKEEAKTTKWYREHPDELKVVYAECQ
                     KTGSASENCKHANEAHWQIQQLNAPEISFN"
                     /name="EexN family lipoprotein"
     gene            complement(15754..16458)
                     /locus_tag="JFX52_RS27760"
                     /old_locus_tag="JFX52_27760"
     CDS             complement(15754..16458)
                     /locus_tag="JFX52_RS27760"
                     /old_locus_tag="JFX52_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010617328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000819778.1"
                     /translation="MKRFRPLILAVLIASPIVNAAGIPVFDATARLENIRQWTKEANQW
                     LQTVQHYKDQLQAYQKQLETVTGIRDIQAFVNQAKSLKADLDKLRKPGQALNDLLLSGG
                     SSGQFDELYNKYKIFDNCNAEQSKSYANTCRQEVINKAIQIEQTNEVQTQIGEMLGEIN
                     ELSDRVANAKDMKEAQDLANSIQLKSVMLNTLTTQWEMNVKAAEKREKMLADERRKKWN
                     QQQLNAPEIMFN"
                     /name="type IV secretion system protein"
     gene            complement(16473..19217)
                     /locus_tag="JFX52_RS27765"
                     /old_locus_tag="JFX52_27765"
     CDS             complement(16473..19217)
                     /locus_tag="JFX52_RS27765"
                     /old_locus_tag="JFX52_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_001096400.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLTVVGVTIIVAIWTSIALLLFLP
                     IEFLVMKSLTRNEPLRFNLIAVWLRVRGNPVANRLFGATAFMPVEHNAVDIQEFIDAMK
                     LNQCATIKKYIPYSSHIHPNVVRSLNSDFFCTWELTGTPFDCESDETLQFGTNQLHGLI
                     RSFEGFPVTFYVHNSREQFTDSLHKVSGNPYADTVSERYYASLNKRPFRRNRLFVTICY
                     RPFASLDKANRKRMKDGQKLKEIDSALLEVLEIKSTLDTALSRYGAHALGTFTENEAVF
                     SSQLAFYEYLLTHQWRKVRVTRTPAYNVMGNVALYFSAESGQINHVNGTQYFRGLEVKE
                     FSEETATGMTDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLMMSADDDAVSQRLDL
                     DVALDLLTSGKIAYGKHHLSIMVFSPSLESLIADTNEISNALNNIGITPVPAEISLSAA
                     YMAQLPGNYNLRPRAGELSSLNFVELASLHNFYPGKRDKAPWGDAMALVRTPSGDGYYL
                     NLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFLQINQQKYNRDDSFSPDAKTKRL
                     TTVYLDKDRGAEINIRALGGKYYRVFSGEPTGWNPFALPATKRNINFIKQLMKILCTRN
                     GATITPRDERRLNDAVNAVMSDEPQYRVYGITRMLENLPEPATREAQENGLSIRLSQWA
                     RGGEFGWVFDNEHDTFDISNCDNFGIDGTEFLDDPNVCAPISFYLLYRITSLLDGRRLV
                     IFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDEIAPAVIEQCGTQ
                     ILAANPSADRSHYVDGMKFEPEVFDVIKGLDPQARQYVVVKNQFRRGDTKRFAARITLD
                     LSGIGRYTKVMSGDAPNLEIFESLYQEGMKPSDWLDSYLAKAL"
                     /name="VirB3 family type IV secretion system protein"
     gene            complement(19230..19526)
                     /locus_tag="JFX52_RS27770"
                     /old_locus_tag="JFX52_27770"
     CDS             complement(19230..19526)
                     /locus_tag="JFX52_RS27770"
                     /old_locus_tag="JFX52_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000608756.1"
                     /translation="MINKLKAICSRASAYILAPLVSVVSPVFAADGFSRANTIMEKVSN
                     GLHGLAAVTITVAVIWIGYKTLWKGESLSQCGYIIIGGILIGGGSEIGALLMS"
                     /name="TrbC/VirB2 family protein"
     gene            complement(19530..20234)
                     /locus_tag="JFX52_RS27775"
                     /old_locus_tag="JFX52_27775"
     CDS             complement(19530..20234)
                     /locus_tag="JFX52_RS27775"
                     /old_locus_tag="JFX52_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000953548.1"
                     /translation="MLSTVSFMAVAMQCAATVHPSTSLDVARVESGFNPYAIAEIIPKR
                     ERQPGDKGFISHMPKTKEDALSIVKQIEAKGRRYSVGLMQITSTNFNSYAVTAADLFNP
                     CTNLSVFEKIITDCYQRGGTLKRALSCYYSGNFTTGQQPEAALSRTSYIQRIGYSPEKP
                     RYVVPGTRDDIATQSAILNATPVEAPTRPRVVWPGAIVRGVPAQLRQKKADTVYYPAQV
                     IRGNRFSTEKED"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(20250..20456)
                     /locus_tag="JFX52_RS27780"
                     /old_locus_tag="JFX52_27780"
     CDS             complement(20250..20456)
                     /locus_tag="JFX52_RS27780"
                     /old_locus_tag="JFX52_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125884.1"
                     /translation="MPKPTYNVYVTQEVPTDENGGKNTYWTKVGAAFAHNGKPGLNIML
                     VPGIAVSGKLVLLEPKEDAPQGN"
                     /name="hypothetical protein"
     gene            complement(20457..20669)
                     /locus_tag="JFX52_RS27785"
                     /old_locus_tag="JFX52_27785"
     CDS             complement(20457..20669)
                     /locus_tag="JFX52_RS27785"
                     /old_locus_tag="JFX52_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001133545.1"
                     /translation="MPNPIGQAKSTAVIYIGKKRYETLAKHARDISYVAEVNIRPSAFL
                     QYMIDKLSNEAHNEMLKELMSKEDK"
                     /name="hypothetical protein"
     gene            complement(20746..21195)
                     /locus_tag="JFX52_RS27790"
                     /old_locus_tag="JFX52_27790"
     CDS             complement(20746..21195)
                     /locus_tag="JFX52_RS27790"
                     /old_locus_tag="JFX52_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998906.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_000995159.1"
                     /translation="MNAWYLLYCNNNDLDRIAYRCKTMNVVAFCPRYIKITPRKDCNAV
                     RKEEKVLFPNYLFLHFDVNVTHTSAITSIPGAIGFVSFGSTICTVPEKVVIAIKCAKIL
                     ALNPDDDSIECRNIGSDLLAEIQRISSIISPLERQTAFSRLLQNQ"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            21450..21815
                     /locus_tag="JFX52_RS27795"
                     /old_locus_tag="JFX52_27795"
     CDS             21450..21815
                     /locus_tag="JFX52_RS27795"
                     /old_locus_tag="JFX52_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248966.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000248966.1"
                     /translation="MVPKRLKEARMEAGLSQEKLSQLLGVAEGINTRSRFSSYEVGRTE
                     PPFKLVKKIAELLDYPENYFYTEDDDFAKAVLDFHRKRTSPELNPNYDSEVEARRMASK
                     LDNVKKIVAQLNEYLKE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            22462..23097
                     /locus_tag="JFX52_RS27800"
                     /old_locus_tag="JFX52_27800"
     CDS             22462..23097
                     /locus_tag="JFX52_RS27800"
                     /old_locus_tag="JFX52_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371526.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA family replication protein"
                     /protein_id="WP_000088100.1"
                     /translation="MSRKRRRFLNRWIKSLTTVAGRIKIKNELRRRIRHNRYWVNEANK
                     FGIEALCELMLAILDDLDLRSWETRHNLETLAERAGLVTRSDAGHKSISRASRGCDRLV
                     WLNAIITEKAPFNPYDARCACKHIEVTEDFFAILGIPLKLVYRERARLLKADPDEVIYS
                     GDARLIAIRVQNWTRKAAAGLARMKAKRDAARQRKKEYYAHSPTPIPA"
                     /name="RepA family replication protein"
     gene            24054..24326
                     /locus_tag="JFX52_RS27805"
                     /old_locus_tag="JFX52_27805"
     CDS             24054..24326
                     /locus_tag="JFX52_RS27805"
                     /old_locus_tag="JFX52_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001272119.1"
                     /translation="MSAFIFRTTFKATGDQQKDAITQIEVMQEQAVKAFVSWMTNQEGT
                     EFLSTLQEITTELDALIDAVESHSPVFRKKATELEALLAHYKTLL"
                     /name="hypothetical protein"
     Typing          complement(5613..6343)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.361"
                     /coverage="100.0"
                     /evalue="9.03e-162"
     Typing          complement(13570..14249)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="85.463"
                     /coverage="100.0"
                     /evalue="4.2000000000000004e-119"
     Typing          complement(16477..19217)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="87.418"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttatattga atcaaccacg caacagattt ttatttttaa tactttgaca ctttgtacta
       61 ttggattaat tcatatagag actggtgaaa aatatattgt agtatttaca gataacaata
      121 agattagaga ttataaaaat ggtgtaatag aagaattagg ggaaattacg caagaagata
      181 ttgaactgat tgagtattat gctaaaaaat atgaaaagta ttttgatgat ttaaaaatag
      241 gtgaggagcc tttgaatctt aaagaatata tagaatgtat gcaatagcca tatattctat
      301 aaaggtgtga tcaggttggt cgagcaacct gatcacttta tcgacactca aaacaataga
      361 gaggtgttca aatgtcaaca ggtcagatcg tacaacttgc ggattcactt gtcaaaggat
      421 gggaaacgag aatcccagca atgaaattta cggattggga tcaattcagg tggtggttaa
      481 attatttaca gggatatgaa atgttttaag caatgttgat gtcctgacag taattataaa
      541 ttttttcaaa aagagagcta aaaaacttgc aatacctgca attgaaggta taattaaatc
      601 atcgagaggg aaatggtttc cctctcatct tagcctcaca ggggcgggag ctacaaaatg
      661 aatatccaag aagcattaaa tatttttggc ttgtctggcc agttgactga aaaagacatc
      721 aaaaacgcct ttaagaaagc tgcattaaaa tatcacccag accgtaatcc ggtaggtgct
      781 gaaatgatga aggcggttaa ttcagcattc gattttttaa tggcgaatct tgataagatt
      841 aatcagtacc aaagtcctga acaaagtgca atatataact atggtgatga actagaaaat
      901 gttttaaagg ctctttacgg tttgtctggt ttagtttatg aggttattgg taactggatc
      961 tggattagtg gaaatactat cgagcacaaa gaagcattaa aagaaatagg gtgtaaatgg
     1021 gcggcaaaga aaaaacaatg gttttatcgt ccagaagaac ataaaagtta ctggaaccgt
     1081 gaagagcaca cgatagagga aatccgcgca aaatatggca caagcggaaa attccaggcc
     1141 ggaggatggc aacaagttga aggcagggca agagttgaag ccagagcata acgaaacggg
     1201 ggcgtaagcc ccctatatcc tcgccagcaa tccttttcgg gcatcgaacc cgaaaaggat
     1261 tgagtagctg acactctgcg attgctggct acgttagcgc agtaaaggat ttgaaagcat
     1321 gacaacattt tcagaaaaat atcagcctgt ctttgaagtt gtatgccgta ttcttgggaa
     1381 cggctggcgt gttaacaaac tggataatta tcagcatcgt attaaactga cttctacaga
     1441 aattcgtaaa tactccatca ttattcgtct ggagcgtgaa agatttttta tagttggtag
     1501 cgttgatagt cttgtatggc acggtaatcc ttccgtctgt actgtatctc ttacgcgcaa
     1561 tcctgttggt attgctaatg atatatctcg gaaaatcctt gctaatgcac tggatgaaat
     1621 tgaattgctc agggagcatg agagcgcatt acaggaaaaa caagataaag tgcgaataat
     1681 caaagggatg ttatcacagt tgctttctat atctccctgg tatggggcat taacgggatt
     1741 taatgcggat aatggacttg caggaaaaat actacaaaat ggtgatgatt atgaaatggt
     1801 tatcagaggg cttaatgttg atcaacttat caaagtggca ggttttataa aaaaactata
     1861 acaaagtgtt gatgtgaatt ttatatgggg ttgtttatga aaactgataa tgttttcttt
     1921 aaaagtaagg gtagttactc ttatgttgtt acagtagata tggataatca gatgtatatg
     1981 gtcagaaagt atgttaagac atatgtgaaa tcttgtgata aagagcaagt taaagggaaa
     2041 aagacagtta aaaaattggt gctcgatacg aatcttacat tgccagaatt attggatctt
     2101 gataatggta ttctggctga tttatttaca agaggtaata acaatgcaaa ctaaaccaac
     2161 tccggcagat gttaaagagg cgagaatttc cgcaggttta acgcttaagg aagctgccga
     2221 aatttttggc tatcagttaa atacctggca aatgaaagaa agcgcaggta aagctacaag
     2281 gtctttatct gtaggtgaat ataactactt attgttgttg gctggaaaac accctgaata
     2341 tgttctttgt aaaagggata cgatcaccgc agaaccaacc actgtctgac cccaaaaggg
     2401 gcttttcgcc ccttttctac taaatgttaa aggccactcg ctttccggta gggagttcaa
     2461 catctaggct caactttcct cccatagctt caacgtaacg ctttaacgtt gccagcttca
     2521 aatcatttcc gcgctgttcc agttgagtta cagcgggctg actgattccc attgcatcag
     2581 caacctgttt ttgtgaaagc tgcaattctt cacgcatcat ttgcagccct gtttccagaa
     2641 tcatctcatc agccatagct ttgatgatcg cctgactttc aggtgattga gccgcaatta
     2701 cttcttctaa tgttctcatt ttttgccctc cagttcggct aagtgagtgg aaaattcttc
     2761 gtcagcaata cgaatcattc tgtcgtaaaa tttcttatca ttacttttgt cgccagcgca
     2821 aagaatgatt gcttgtctaa ttggatcaaa tgcaaagaat gccctgattg ggtgtccctc
     2881 atattgtata cgtagttcct tcatattctt atgacgtgac cctttcactg tatctgcatg
     2941 tggccgtgaa agagaggggc caaactcttc aagatgttta agttcagcca gcacttttcg
     3001 ttttagggct agttcctgct cttcaaacca ttcatcaaat aatctgccaa aataaacagt
     3061 ccacatgtcc cttttcctat aagttatagc ttatagatga ttgtataagc tacgacttat
     3121 aatttcaagg gaaacggggc tatttaacat aatggacgtt atcggcacca tcggatcacc
     3181 gttcgttgtc ggatctgcaa aatccgctca aaagatcatg ttttcctggg cgttttccac
     3241 cgttttttat gttgcgtatt tgttaataaa acgtcatttt ttgttttggc tgaatttggt
     3301 tgtgaaagcc ttatattcac aagttatctc ttcacttgcc gcctgtagtt caaatagttg
     3361 ttgcaattct tggcaccaat acggatacta taattatatt tttctataaa aatattttta
     3421 tagggggtcg tatgacggta gctattgcgc tggttaacgg taaaggcggg gtgaaaaagt
     3481 cctcaatgag cagggctatt gccacagagt acacgaaggc agactggaag gtattagggc
     3541 ttgatatgga tgttgatcag gggtcattcc tggattgggg acagcggcga ttgagatata
     3601 aagttgaacc acctgtcact attcagggat gcggaacggt atcacaggct aaaaaacata
     3661 ttgaatcagg gatatgggat ttagttgtta tcgatagtgc tgcttatgca actaaatcgc
     3721 tgttgagcct ggcagaaatt gttgatttgc tcgtgttacc gtctggtttt agtgtggacg
     3781 atttgagaac gacggttaca accgtgaatg cattgcgtaa caagggacac tcaacaaata
     3841 agatggctgt agtttttgtc ggagtcagtg aaagcgaaag tgagtatcgt accgcgcttg
     3901 aatatcttaa accgtgtggt gttcaagtta tcaatggcgc aatacctttt atgacctcgt
     3961 tcagccaggc acaggataaa ggactggcat tgacggaatc cccttttcca ggtccacggc
     4021 agaaagctga cgaagttgtc cagggagtta ttgacttgct gactaaactc tcacagtgat
     4081 atataattct ataattatat ttttatagag gtgataagat ggctaatgta ccaccaaaac
     4141 cgcgtaacac caaaggcgag ccgccaagtc tggaggatac acaacacgga tttaatccga
     4201 cgcgtaaaaa cgctgatggg agaaaagctc agcttaacct ttatgtggat atggaagtta
     4261 aacgggagtt ccgctcgatt gcagcggcta atgatatgaa gcacggggag ttgctggagg
     4321 cataccagga attctggaaa aagcatcatt aaaatggcat aggggacgaa ctgtcccctg
     4381 atattaacac ttaccaacat aatgaatatg ttcaacggtt cctaaaatct caaaacgatc
     4441 gggagctacc tttcgccagt gatttttggc acgagaggca tagcttctgt cccagcggag
     4501 aatacgatta ttgtcctgga taacacatac catcctgttt tctgctgtac gcaatgattt
     4561 tgtaatttta gctaaatcat tatctggact tagatcaatt agtttttcat ggccgtcatt
     4621 tgacacaacg agtattttta tcgtcattat cgtctccatt atcatattct ggaaacacta
     4681 ttagtaacgc ttcgccgttg gtgcgtttca taaaattgaa cactaatctg cctacttctt
     4741 catttgtcat atagcgttct attggcaatg tttcgtattg atccgcatag agaatattca
     4801 cactttcttc ttctggttct gaaatctggc caattgtgat atctgtcaga cctgtatcag
     4861 tgaaatttcc tgattttatg tcaaagtcgc tatctttgaa tttgtatgca tcctgccata
     4921 cagcgaaaca agtgtggcag gtgatgggca gttcgccttc agccagcttt tcacaatttt
     4981 gaaccagtac tcgctcttgg ttgtactgta agttatagcc acctgtacaa agcgtttcat
     5041 acccatgagc gccagttacg atatgtgtcg caaccctttc caggactttc ccattcacta
     5101 ataactgttc acgtgtggta attgtttgca tgtcaatctc ctatttatgt cgccattccc
     5161 acggtggaac gggattgggg tcagcccaca aacctcgttt ttgtgagcga gcttcctgtt
     5221 gtagatctgc cagagcgtta tctgtgttgt aatgctcata cacccatgca gcacccgatt
     5281 gcaccataaa gcggttagcc tcactaccgc ctgttgtgaa tacacgtccg ataatgcgac
     5341 cgtaacggtc gttgtgtgaa tatgtcaccc tgacggattg tccggcaatc aggcttttaa
     5401 gttggtcagt tgaccagcgt ccgtaagcct gtttcttttc cggtgcatca atattggcca
     5461 gacgaatcct tactggcgtt ttattctgta aaatttcgat ggtatcgccg tcaagtacac
     5521 gaataacttt tccctgtata tccgctgcat tggcatatag tgaaggcgac aagatatata
     5581 atgcgaatat gattaatatt ttcatcaact attgttccag ttcatcaatt gttctaaaaa
     5641 taaacatctc tctgtttttt gcatcaaaag gcacaggtgt attattcctc attacagttt
     5701 tgattgagtc gaacagtctg attaatgatt ctttactttt ggtgtcaggc atgacgtaca
     5761 aagcataatg ccagtttcct ttatctcttg ccgtaagatg actgtttata atgcttatat
     5821 agcgagcacg agttttcatt gttcgttctg tttcaatagc cattattgcg ccagttggtt
     5881 gttttattac tccatcaggg cgatgggaac agccggaata acgtgcgatg aaggctgcac
     5941 tatcaccgtt aatccattca gtagcgcctt tactttccag agatattctc actcgctgat
     6001 ttaaaagacg atgctcaaga gtccagtatt ttagtttgct gggttcgaaa taactaggga
     6061 acgtaatgtc atcggctttt acaaccattg ctaatccttg catagtaata ccccaaacag
     6121 ttaattttct ttttgatgat ggtatttcat gtttgataat aaaaccttct ttaagagcct
     6181 tgtttagtaa tttatatact gggtttctgc ttttttttgt gaatcctaat tttcttttta
     6241 taatgtgaaa gtctgaatac gtttcatcct taaggaaatt taaaagacaa agcatgttgt
     6301 tagctgatat tctttttctt tcgtaaaaat catgaattaa catagtaaaa cctcataaac
     6361 cactggaagg cacatcgttg ttattgtgtt gttctatttg ttctgtctca aaagatagca
     6421 attcaatggt ttctttgttt gctggtatat gagacatttt agttgctttt ggtaaatctt
     6481 ttgatgtgaa cacaaatcct actctttcag gtagatttaa aaacatatta ctgtctacat
     6541 aataactttc tgcctgtctt atcatgcggt cagtatcaac tttttctgtt aatgataagt
     6601 cggttctcac cttcctcatt tcatcgtcaa ctaatattga accagtcatt ttagctgccc
     6661 actctgctgt ttctggattc tggattttat agattaactt gaattttgcg ttttcaataa
     6721 cagcaccaat tacagaatcg ccattaagat cagaagggca atctcttaaa tcatctattg
     6781 cctggaaggc cataaatata tgcacacttt tatctcgtgc tgtacctaat ccttccattg
     6841 tcgggcgtga aatatgatat ttcaactcat caagaaaaat tgcaacaaca cgtggagttg
     6901 tgtttattct gtctctggtt tcagctatct gaatcaaacg cgtaagtatc atgcgttgtg
     6961 cagaaataat tttttgattt cgggttgacc caatgatata gcagcatcct ccattatcaa
     7021 aaatctcttt aagagaaaat ccattttttg cattgacgga atttacaaga gcaatttctt
     7081 ctagttcacc taaaaatgct ggggcatctt ctcctattga ttttacgttg tcatcgttga
     7141 ataaatcata cagtgtcatt ccggttttat gaattgcggc tacatttctg gctgcgcggc
     7201 gatcagctat acgataaaaa tctgcggcct ctcctttttt tgatagacta aatcctgcat
     7261 taaataattc ttctaattgt tcatgtgata tgtcagcaag taagtcaagt tgatggttgg
     7321 ttctgtttaa attaattaca acaaatttct ttcctgcttt tttacatgct tccctaagaa
     7381 catgcacagc ccattcatca tcttttggat cttcaacaaa aacagcctcg cctaacagaa
     7441 ttgcctgata aagcaaaacc gatgcggtaa tgcccttacc ggaacctgtt gtacctatga
     7501 ttgctgcatg ttgtttctga aattctttta ttgttatata ttgcggttgg tcgtctttgt
     7561 ctaagccaat gaaaacccct tttttcaaat caatgtaatc taggggattg tattctatgc
     7621 tgtctggcaa caattctttt actttgcgaa catctgttct tgtatttctc tcaagtttgc
     7681 ttttctttat cattctgtgt ttcaggctgt ctatttcacc tgctaataat cgtcgcgcgg
     7741 tcaaatgaaa agctaatcca gctagtgtaa agagaattgt gattatccat aatggtattc
     7801 taagtagtgt attgggatcg agaacgtcaa ataggtagct cagaccatga atactaagtg
     7861 gtgacattgt gccgaatatg aaaaagaaaa tggacacgca cgcaacagat tttaaccaga
     7921 agggagcgtt ttttctttcc tctcgtggta ggttgaatat aaatggtagt gttagtcctg
     7981 ccagtaatga caatatgaca gggctgttct gtagccataa cagaaatatt gaaaaggcat
     8041 cagcaatacc attcagcttt tgcattagat gtggattcat atttgttttc cccattcata
     8101 aagtgaatct atggggtacg acaatggggt acagtaatgg gggatataat gtaccccata
     8161 gctgtacccc attattgtac cccaataaaa gccaaaaacg ttgtgatcgc ctaaggcgat
     8221 catgcccgtt atccgttctt tcagaagcca gccgataagt tatcgtctgg ctcctgaaag
     8281 cccgaagaac gggcaacggc gtgtgccgtt tcggcctggc tgaaacgcca gtccttcacg
     8341 tcacatgggg gtaatggccc gtctggacgc cggaccaaaa cccccaaggt caaaggcggc
     8401 acaccgtcgc gcgctacgcg cgaccacccc ctttaagacg cgcgtttagc ctattttttc
     8461 ttcgcaagct cgaaaaaatt ggaacgctgc tttaaagggg gtggtcgcgc ctaatcggta
     8521 atatcccccg ctggggcggg aaatattacc tggcccgacg gtgtgcgggg caagcccccc
     8581 acaaaaaccg gcacgtgggc gtgccttttt tctcgctggg gcggaaaaat ttttgcggct
     8641 ccccccggcc cgcttcaatt cgcttggggc gaatgttcgc gggtgggcgt ggtgcctgaa
     8701 aattaacaat ttcttcgaaa ttgaattttc tgtccacgca ccactttctt tattcctgtc
     8761 ttaacaattt cttcgaaatt gaagccagaa ataaagaaag cctgaactgc accacgccct
     8821 gcgtcggact cgcccaaatt cgcaaagcga atttgccccg cccttcgggt tgctacgcaa
     8881 tcctctgcct cagacctgtg cccgtgttat ttcagtcaga cattcatcct cggcttttat
     8941 tgcagcatta tatctgtctt ctgttatttc ataaccttgt gaagcagcgt aactaatcag
     9001 cttattggca tcagaacttt tcagttttac tgtgtgctca attaaccctc gcattaacct
     9061 cgcttgatat atttcataat atagcccgca tcccctgttt gctttattac taatatcatc
     9121 aaagactctt tttacatgga ttctgtcctg attccttgaa tgaatattat taatgaacat
     9181 atcttctttt aacataggat tctccagatc tgaaagaata tgttaatttt ccctcttcga
     9241 ttgcaatgct aatgcttccg attctgtaat gagaataata tcaggatatc gtttctttaa
     9301 atcttcaagc gtttcgccga tagaagtaat gaaattccct ttcattcctg atggaatgga
     9361 gcacactatc ttgtgtgcat agctataaac aagatcggag tttgtcagat aaaataccat
     9421 gttttcatct gtattcatat ttaccctcga actctaccaa acttactcat tatttttgat
     9481 atagctgcgg gatcagcgga tgaacactga tttaaaaatg ctttacgtgc atcaaatgtt
     9541 ttactccatc gtattgaacc tttctttttc ttaagaatgt tgaagaatgc tttttctgct
     9601 gatctgcatt cgccacctcc gctgtttcct gtagcttttc cgtacataca taaaacaacc
     9661 tcgcatgggt ctgcggcata agagtaaaca ggtgtggcgg ttgttaaaac aaacattgtt
     9721 attgctgcaa tttttttcat gtcagaaccc tttgtttaat attttgtgtt aacgcgaaaa
     9781 gtagatggtt gtttcattgc acgtaagaca atttgtgatg gggtgatata taattacccc
     9841 gtttgcacca tgatgataca accagttttt tgcactgaaa taatcagcgc ctgactttgt
     9901 tgctactgtg atttgtgttg agtgagcaat ggcataaaag acagatggca tccaatcttc
     9961 tttattgaaa tcttgtgagc taatggataa cgtccatttg tcattaataa caggcgattt
    10021 tattattaag ctattggggt tccattcagg aagtttctcg ttaagcgata ccggcttttt
    10081 attccattcc acgggtactg gtggaggcgg tgaggaacat ccggtaatta gtgttacaag
    10141 cacagtaacc agtatatgct ttatcatact ttcatttcct cgtaatattg attgcggtgt
    10201 aacggtcgga aataaatatc attcatacgg cgtacgtttc catctaaatc aatgctcaca
    10261 acaacatcca gagagtcgag aactttacgt agcaagaatg aatatggtaa ttgcgcacac
    10321 tgtgggtttt cagtacagcg ggtaataaaa ccgtcgattg cttcatgggc tgaaccagca
    10381 tgaatggagg tcatacttcc ttcatggcct gaccccgtta ttttcagaaa atcccatgcc
    10441 tctccacccc tcacttctgt taataaaatt ctgtctggat tcatccggta attccagcgt
    10501 ataagcgccg ctggtgttac aacagaacct ttttcatcgg atgactcaga aggataaaac
    10561 agatgaacat agttcttatg tttgtagaat attatctctg ggttgtcttc tatcgtcgtt
    10621 atgcgtaaat ggacgggtat atattgtatc aacattttca tgtatgttgt tttacccgta
    10681 ctggtttcac ctgcgacggc taatgttttt ccatactcca cacatttttc cataaacaaa
    10741 ggaatgtttt ttgatttgta taattcaatc aattcttcgt tatgtgtgga tgctttttca
    10801 atgcctgtga cgcggttata aaaaccgcta tcaatatatg cctgatgcgg aatttgaatt
    10861 tttgacggtt tacggatcgt gattgagatc gtgtcacgtt cgcacgccgg aggtacgaca
    10921 acctgacatc gttctcctga ttccagggtt gcagaaagtc ctggtttgat atcctcgata
    10981 ttatcgttat ggtgttttgc aagacaccga gcaaagttat agcaatcgtc aaatttcaga
    11041 tcaacggtat gttgccccca gacaccatct atctttgtcc agagttcacc tggacgatta
    11101 acagctattt ctgtcagtcc tggtaattcc agaaagtcac caaaaaatcg ctgtttatag
    11161 cggtccaggg aaatattctt agcggtcatc gtttgctcct ttcagtgtga ttctggtggt
    11221 acaccgctgc aaaacggcct gtgcggtttt gtttttaatc agacgctggt aagcatcctg
    11281 tgcggcggct tcgttcgggt tcccggcgag accccccatg tgtccggcgg cgagcatgtg
    11341 caaccaagtc tctttatctt tgcagttcat ttttcagcct cagcgtgtag atatttgaaa
    11401 agtcaatatc ctgccctgta atcaggttga taatttcgcc ctggtttttg tagagcgtag
    11461 gggggatatt gatactgttt tccagcgtgg ttcgagccat atccgccata gcctggcggc
    11521 tgttctctgt atagtctgta ttgcggtctt ttttacccgc attgtttgat gcccatgcgc
    11581 caatatctgg gatcatgccg accattaatg cgccgccaaa cctctcccag aagtgggtat
    11641 caatccatcc atcaacacct gattctccaa gctcgccagc ggcgttggta tcaatcagcg
    11701 gaatatccag atatggtcgc tgtcgggtgc gtaattttgt catcatgata aaagcgcgcc
    11761 cctgcccttg cgtaattcct gtctggtata ttcctgttgc tgtggttccc ttctcgataa
    11821 gttttgtatt gccgctggca ctccagatat ctttagctac cgtacatcgt atttttccag
    11881 cgcgatcgga aacaaagcgg taatccatag agcatggaat gactgtgttt tccggtatgt
    11941 acaaatcagg gttgtagggg attcttcgca ctgttgcggg ggaaattgcc tgtgatgcag
    12001 tgccatgttg tgttgtggtt gctgtactgt ctgtaggctg tgtggcgttg ttttccgcag
    12061 ccagtatcat ttcctgttgt cttgttttta cctgattttg ggggctggtc gtattattac
    12121 gacgaacaag gaagctggcc ttattcagtt gtggtggctc aaatgttttc atctgtccgg
    12181 cagtaccgga tacattgccc gtgttatcct ggctaacagc ttgctcaccc tcagtcgaat
    12241 tttccggctc attggtggcc atgcctaaat cggttcgttt ccggtagtca ttcgtttttt
    12301 taggtggtgt aacctccttt gttttctctc cattttcttg cttcaggaag ttttgatata
    12361 cccagttacc gccccatgtc aggccaatca gggctactgc gcatattgcc agaaaggccg
    12421 ctgctttccc cttctgacgt tttttcagtt tgtttactgc tggttgtaat gagttctcct
    12481 tttcatcgtc ctgtgatgct tgtaattcag cctcacgttt tgctctggct tctcgttcca
    12541 gcgtggcgat atcatcctga tcagatgtca tcactggctt ttcttcttcc gtagaggggg
    12601 atttattttc gtcagtcata tatgagtcct tatataaaaa tataattata tttttataga
    12661 ttaggtgtta tctgtttttt caacgcgctc caccttgtca gatacggttg aattatcagc
    12721 cgcgtgtact tttccaaagc cgttattttt cagccccact acggcatcac cggaacgtaa
    12781 tacaagccgt ggtgtgattt cctggataac gagaacggta aaattgccct tacgctgtat
    12841 actgctgtta accacgtatt cctggccgtt ctgttctttg gtaactgatg gaactgattt
    12901 gttgggtgag aatccgagaa acgtaaagcg accgtcgtcg taggcaaaat caggttcaat
    12961 gttgtaactg tcttttcctg gatatttcgt gtagtcccag ttacgcgggg tttgtgcggt
    13021 tctcagtgct ttctgaatct gggtttgttc ctgcttttgt tcccattctt tctgacgcgc
    13081 tgctgcttcc ttattagctc tgttacgttc gtctcctgga tagcggtaac tcaccaggta
    13141 tgcgggctgt tgtggcgatt tttcatcaat gacattcagc tcaacgttgt acaggcgctt
    13201 atttgtcaca atcagcatat tggtatgcca gtcacggctg ttcggcggga taacaacctg
    13261 tgttgtatta ccgtcttcac caggtgcgcc ctgtgtgagc gcaaccggac gaatgtttac
    13321 cctgtttgca tctgctctgg cttcccatgc ttcatcaaag ccaggactgg cactgataac
    13381 agcttcatcg ttatcaaaaa ccagagtcgt cataaatcct ggttttgtgt ttacaacggt
    13441 aacattctgc gggttataaa tgacctgctg gatacgtgca tcataacggc tcgcttgtgg
    13501 tgtagctgcg gcccatgcct ggctgttcag cattattatc agggtaatta acgtcagttt
    13561 tctcattact cgcctcccct gatttcgcgg tcagtctgcc agcttgttac ggtgaatccg
    13621 aacgggttta tttcccgctc aacatctgac atttcttttt cagggtctga atggaaggtg
    13681 aaacgtgcat cccagtattc attgcgaata ctgttatcag ccatgcgacg aattattttt
    13741 ttataacgaa cggtggctac tttgtctggt gctgttgcat tcgttatctg ctcagaaata
    13801 atttcaattt tcacggtgtg agccgcgtta ttataaactt tgtctggtgc gttttttccg
    13861 gcgtacagtg ccaggtagtc gtcgtttacc tgtggtgagt tgtagatctg tactgtctcg
    13921 taatcgtcct gtaatgcagg ataaacgtac cgttcacgta atttaacgta attggcggcc
    13981 atagcttttt ggtaggcttc ttccgctgtc agggctgttt ttgatgcacg ggtaacgtgt
    14041 tccactcgtc cggtatgatt atctacggta tagagttcaa ctgttgtttg tttaagtggc
    14101 aacataatca ctatcgcgat cagtgacagg atagtgatta ataaccctgc tccaccgacc
    14161 agaaagcctg cttttgtggc gcgtttatcc ctgtcaatca tttgctgctc aaacgagcgg
    14221 gaatcagcaa ttaatttctg ttcagtcatg tgtggttagc tccgcgataa tttggggtgt
    14281 gtttacaggg ctaccctgac cgctgaatgc gtgcggcaga gatttagatt tttgtgcgca
    14341 gccagaaagc acgcacagca aagccagcgc gattaagcgt ttcattttat gtaccttttt
    14401 gtatttctat aaaaatataa ttatattttt atagttatct tggaggaggt aaacgtttaa
    14461 ttgatggcgg tagttttggt gctgattgat ctgtcagcat ccgcgcttca ttacgcgcta
    14521 tcatttgttc tatagcgtgt ttacgtgcat cagcagcgtt aaatactatg cgcgattgtg
    14581 gatctcctaa ccctttatta cttgggcttg ctacttcttt aggtaatact tttcctgcct
    14641 cctttaacat ctttccagcg ccgtatgcag cagcgccaat gccgagagcc gccgcacctt
    14701 gcatagtggc tgtagcactt gctcctgcaa gtgccccagc tagttttgcg gacatgtaca
    14761 ccagtattgc agcgcatact ccggcaagac aaacttgagc gccaagttct acaatgtttg
    14821 ctttgctggc ttgggtcact gcttctgaca ttctggatgt aagaaaacca attacaattc
    14881 gcagagctaa ggctgaaaac ataattgtta gtattgcagc aaatatattt tgaagccagt
    14941 tattaaacat tggctgtaaa aagccataca tcaggcaaaa tataaatatt ggtgcggtaa
    15001 tacctaacaa taagatcatg atctccgcaa tcatagatac gaatgctgtg ataatcatta
    15061 agacaaagac accaccccat acaaataatt cagcaattgt gccttcctct ttgataaatt
    15121 cggagttgtc tagatcatgt aacgttttac ctaaaactct tgcgctgttc catagagtat
    15181 caagtaattg ccagatgctt gtgctactgg aaaaaccttc ttttattcca tttacagcat
    15241 caatgacacc atcaagatat ccgttaatgt ttgctacaaa ggataaaatg atagccatcc
    15301 tcattatatc ccacataaca tcttccattg gtgtagacag ctttcccgct aaggtttgat
    15361 atcctcgcca cataatgtag atagtggctg acgtagccgc cagccccatc atcattgacg
    15421 aataagacat catcaaacca ttagtcgcgt cagttatccc accgatgata tttttttcga
    15481 cagaagaaaa tataccggcc atgataaacg ccctttagtt gaaacttatt tcaggtgcat
    15541 ttaattgttg aatttgccaa tgagcttcat tggcatgttt gcagttttca gatgcgctac
    15601 ctgttttctg gcattcagca taaacaactt tgagttcatc aggatgttca cgataccatt
    15661 ttgttgtttt agcttcttcc ttacatcctg aagttaacgc catcaggcct gaaataattg
    15721 taaataaaat gattttcttc attaagtcac ctgctaatta aacattattt ctggtgcgtt
    15781 taattgctgc tgattccatt tcttacggcg ttcatcagca agcatctttt cacgcttctc
    15841 cgccgctttt acgttcattt cccattgggt cgtgagcgta tttagcatca cagattttaa
    15901 ctgaatactg tttgctaagt cttgggcctc tttcatatct ttggcatttg ctacacggtc
    15961 tgataattcg ttgatctcac caagcatctc accaatctgg gtttgtactt cattggtttg
    16021 ttcgatttgt attgctttgt tgatgacctc ttgtctgcat gtattggcat aactttttga
    16081 ttgttcagca ttgcagttat cgaaaatctt gtatttgttg tacagttcat caaattggcc
    16141 agaagaaccg ccggataaga gcaaatcgtt cagggcttgc cctggcttac gtagtttatc
    16201 caggtcagct ttaaggcttt tggcctgatt aacgaacgcc tgaatgtcac ggatgcctgt
    16261 aactgtttcc agttgttttt gatacgcctg tagctggtct ttatagtgtt gtaccgtctg
    16321 tagccattga ttggcttctt ttgtccactg gcgaatattc tctagtctgg ctgttgcatc
    16381 aaacacgggt ataccagcag cgtttactat cgggctggca ataagaaccg ccagaataag
    16441 ggggcgaaat cttttcatcg atatctcctt ggttatagtg ctttagctag gtaggaatcc
    16501 agccagtcgc tgggcttcat tccctcctga tagagtgatt cgaatatttc caggttaggt
    16561 gcgtcgccgc tcattacttt tgtgtatctg ccgataccag acagatccag cgtgatacgg
    16621 gctgcaaagc gttttgtatc tccacggcgg aactgatttt tgacaacgac atactgtcgc
    16681 gcctgtggat caagtccttt aatgacatca aacacttctg gttcaaattt cattccatcc
    16741 acgtaatgag aacgatctgc gcttgggttg gcggccagta tttgagtacc acattgctca
    16801 ataactgccg gagctatctc atccttgatt atttccgctg gagattgcgt gccaacaacc
    16861 agcataccgt tcagttttcg gatagttttc agcttgttat acgcaaaatc cttaaataca
    16921 gggtcgcgta accacttcca gaattcatcc atgaaaataa caagtcgtct gccatccagc
    16981 aggctggtta tccggtacag cagataaaac gagatagggg cgcagacatt cgggtcatcc
    17041 agaaattcag tcccgtcaat tccgaaatta tcgcagttgc tgatatcgaa agtgtcgtgt
    17101 tcgttatcaa acacccaacc aaattcacca ccgcgcgccc actgtgaaag acggatactc
    17161 aggccatttt cttgcgcttc acgcgttgcc ggttccggta ggttctccag cattcgggtt
    17221 atgccgtaga cgcgatattg aggctcgtca ctcatgacgg catttacagc atcattgagg
    17281 cgacgttcat cacgtggcgt tatggttgca ccgttgcggg tacaaagtat tttcatcagt
    17341 tgcttaatga aattgatgtt tcgcttagtg gctggcagag caaacggatt ccagcctgtt
    17401 ggctcaccgc tgaataccct gtagtacttt cctccaagtg cgcgaatgtt gatttcagca
    17461 ccacggtctt tatcgaggta aactgtggtt agtcgtttag tcttggcatc cggtgaaaag
    17521 ctgtcatcac ggttatattt ttgctgattg atttgtaaaa acgtcatcag cattgtttta
    17581 cctgagccgt ttgtacccag aatgcaggta cttgcaggat ttttttcgtt gaactcgtct
    17641 ttatctgcca gagtgttatg cagattcaga tagtagccat cacctgacgg ggttcgcacc
    17701 agagccatcg cgtcacccca cggggctttg tcgcgttttc ctggatagaa attatgaagt
    17761 gatgccagtt caacaaagtt caggctactc aactctcccg ctcgtggccg cagattgtaa
    17821 ttgcctggta attgagccat ataggccgca gacaatgaga tttcagcagg aacaggggtt
    17881 atccctatat tgttgagtgc attgctgatc tcgtttgtgt ctgcaatcag actttccagt
    17941 gacggcgaaa acaccataat tgataaatga tgtttaccgt aggcgatttt accggaggtg
    18001 agcaggtcaa gcgccacatc gagatccaaa cgttgagata cagcatcatc atcagcactc
    18061 atcatcagcc gtcgggtgcg cttaatcgcc tttttcgctt cctcacggga catgcaggta
    18121 taggattgtg taatcacata atcgcacggc gcgtacagta aggagtctgt catgcccgtt
    18181 gcggtttctt ccgaaaactc tttaacctcc agaccacgga aatattgagt gccattaaca
    18241 tgatttattt gccctgattc ggcggagaag tacagcgcaa catttcccat tacgttgtaa
    18301 gcgggggtac gtgtaactct gacttttcgc cattgatgcg tgagaaggta ttcataaaat
    18361 gccagttggg acgagaatac agcctcgttt tcggtgaaag taccgagcgc gtgagcacca
    18421 taacgggata aggctgtatc cagcgtactt ttgatttcca gcacttcaag taatgcgctg
    18481 tctatctctt ttagcttctg accgtctttc atacgtttac ggtttgcctt atccagactg
    18541 gcaaacggac gatagcagat ggtcacaaac aggcggttac gtcggaatgg ccttttattc
    18601 agtgatgcgt aatagcgttc gctcacggta tccgcgtaag ggtttcctga aaccttatgc
    18661 agtgagtccg taaactgttc acggctgtta tgtacataaa atgtaaccgg aaagccttca
    18721 aatgagcgta ttagcccgtg caactggttt gtaccgaact gtagtgtctc gtcagattca
    18781 caatcaaacg gcgttcctgt cagttcccag gtacagaaaa aatcgctatt cagtgaacgg
    18841 acaacattag ggtgaatatg tgacgagtaa ggaatgtatt tttttatggt ggcacattgg
    18901 tttagcttca tcgcatcgat aaactcctgg atatcaacag cattatgttc aacaggcata
    18961 aatgcggttg cgccaaataa acggtttgct accggattac cccttaccct tagccagaca
    19021 gctatcaggt taaatctcag aggttcattt ctggttagtg atttcataac cagaaactca
    19081 atagggagga agagaagtag ggctatggaa gtccagatag caacaataat tgtcacgccc
    19141 acgacagtca gcaggggcac cattggcacc ccccacagcg ccgccggacg tttcatcgcc
    19201 ttaagcagct ttgccatatg ttcggcctct tagctcatta gcagagcgcc aatttcactg
    19261 cctccgccaa ttaaaatgcc gccaatgatg atgtaaccgc actgcgacag gctttcgcct
    19321 ttccacagag tcttatagcc aatccagatc acagcgacgg tgatggtgac ggctgccaga
    19381 ccatgcagac cattcgaaac cttttccatg atggtatttg ctcggctgaa accgtcagcc
    19441 gcgaaaacgg gactgacaac acttactaac ggagccagaa tataagcaga tgcgcggctg
    19501 caaatggcct ttaatttgtt aatcatcggt taatcctctt tttcagttga aaatctgttt
    19561 ccgcgtataa cttgtgcggg gtaataaacg gtatctgcct ttttctggcg cagttgagca
    19621 ggaaccccgc gcacgattgc gccaggccag acaacgcgag ggcgtgtcgg agcttcaacg
    19681 ggtgttgcat tcagaatggc cgattgagtt gcgatatcat ccctggttcc aggaacgaca
    19741 tagcggggct tttcaggtga gtaaccaatt cgctgtatgt aactggttct ggataacgca
    19801 gcttctggtt gttgtcctgt agtaaaattt ccagagtaat aacaactcag cgcccgtttc
    19861 agtgtgccgc ctcgctggta gcaatcagtg atgatctttt caaaaacaga aagatttgtg
    19921 cacgggttga acaggtcagc agccgttacg gcgtaactat taaaatttgt acttgtgatt
    19981 tgcatcagac caacagaata gcgacgacct tttgcctcta tctgtttgac gatgcttaat
    20041 gcatcctcct tagtttttgg catatgtgag ataaagccct tgtcgcctgg ctggcgttca
    20101 cgtttgggga tgatctcagc aatagcgtag ggattaaaac cagactcgac ccgcgcaaca
    20161 tccagtgatg tggaggggtg aactgtggcg gcacactgca tagccaccgc cataaatgaa
    20221 acggtggaaa gcatagtacc ctcgctaaat tagtttcctt gtggggcgtc ttctttaggt
    20281 tcgagtagta ccagcttgcc ggaaacagca ataccaggaa cgagcataat attcagtcct
    20341 ggttttccgt tatgcgcgaa tgccgcgcca accttggtcc agtaggtatt tttaccgccg
    20401 ttttcgtcag tgggaacttc ctgtgtgacg tatacgttat aggtgggttt gggcatttat
    20461 ttatcctctt ttgacattag ctctttcaac atttcgttgt gagcttcatt acttaatttg
    20521 tcgatcatgt actgcaaaaa ggcacttgga cgaatattta cctccgcaac ataacttata
    20581 tctctggcgt gttttgccag tgtttcatag cgttttttgc cgatgtaaat aacggctgta
    20641 gatttagcct ggcctatagg gttcggcatg agtttatagc tcctgtatga aatatccgtg
    20701 tgcaaaagca cagcggtacg actggagaag gaatgaaggt ctggtttact gattctgtaa
    20761 aagcctggag aaggccgttt gacgttctaa cggtgagatg attgaagata tgcgttgaat
    20821 ttctgccagt aggtcactac cgatgttacg gcactctatg gaatcatcat caggatttag
    20881 cgccagtatc ttggcgcatt taatagcgat aacgactttt tccggtacag tacatattgt
    20941 ggaaccgaaa cttacgaagc caattgcacc tggaatagag gttattgctg atgtgtgcgt
    21001 gacattcaca tcaaaatgga ggaagaggta gttaggaaat aaaacttttt cttccttgcg
    21061 tactgcgttg caatctttcc gaggtgtgat ctttatgtat ctcggacaga aagccacaac
    21121 gttcatagtt ttacaacgat atgcaatgcg gtctaaatcg ttgttattac aatatagaag
    21181 ataccatgca ttcataagcg tcaccttacg gacacaagcc cccattttcc gccgtcctaa
    21241 gttgccgggc ttaggctgga gtactcatta agtgttaact gtaaaaatac acagttgtga
    21301 ttttatacag caattcttgc gtgtcaatat caagaatcta aagaaataat agcattactc
    21361 gattcaaaat caaccccgat tcattatgtt gactttgagg cctgtaacgc cctgtaagat
    21421 gggattccca aaaaaaggga ggttgtatta tggtgcctaa acgactgaaa gaagcgagga
    21481 tggaggctgg attatctcaa gaaaaattat ctcaacttct tggtgtagcg gaaggtatta
    21541 acactcgatc tcgattttca agctacgagg taggcagaac tgaaccacct tttaaacttg
    21601 taaaaaaaat cgcagaactt cttgattatc ctgaaaatta tttctataca gaagacgatg
    21661 attttgcaaa ggctgttctt gattttcacc gaaaacgtac gagtccagag ctaaacccta
    21721 attacgattc ggaagtagaa gcaagaagaa tggcatctaa gctggataac gtaaaaaaaa
    21781 tagttgctca attaaatgaa tatctaaagg aatgagtgct taggcatcag tgcgtatgtg
    21841 ctccatttgg tcaagtcgct tttccgcttc ctcacccact cgctcactaa cgttcggtcg
    21901 ctagggttgt ggcgagcggt aaatacccta ttaaattccg ttatttttcc gtttgttatc
    21961 gccgatttct tttcgatatt aatgctaatg tatgcgtaca ttagcattaa tatcgtgagt
    22021 tatatgctgt acagtattta atctcaattt tgttaagaat tatataacca gatgaagttt
    22081 aaagaaaaat aggaaggtaa tcggcgggat cttttgtgga tgaattgatc ttcgaatggg
    22141 ttcatgctat tttcgtgcgg tttttacgga tttttgggtg ggacgttccc gtaacagcgt
    22201 ctaggggaag ggggggaagc attttagccc ccctgaatgc aacaaccccc tgatatctct
    22261 cttcgatttt gccggtccaa gaagataatc agggggtcca aagcaggctg ggtgcctgca
    22321 aaggattata gcaaatgcat ttctcaatac aagacctctg tccggcaata agtgcgggcg
    22381 gtggtggctg aaaagaacaa ctccttaatc ccccgcatat cgccgtcagg ggtggcaaaa
    22441 caaagtccat gctataacgt tgtgtcgcgt aagcgacgcc gtttcctcaa tcgctggatt
    22501 aaatcgctga cgacggtcgc agggcgtatc aaaatcaaaa acgaactccg ccgccgtatc
    22561 cggcataacc gctattgggt taatgaggct aacaagtttg gcatagaggc tctttgcgaa
    22621 ttgatgttag ctattctgga cgatctcgat ttacgctcgt gggaaaccag gcataacctt
    22681 gaaacgcttg ccgaacgtgc cggactggtt acacgcagcg atgcgggaca taaatccatt
    22741 tctcgcgcca gtcgtggttg tgatcgcctg gtttggttga atgccatcat tacagagaag
    22801 gctccgttta acccgtatga tgcacggtgt gcctgtaaac acattgaagt aaccgaggat
    22861 ttctttgcca tccttggcat tccgttaaaa ctggtttatc gggaacgtgc caggctgtta
    22921 aaggcagatc ctgatgaagt gatttactcc ggtgatgcca gacttatagc tatcagagtt
    22981 cagaactgga cgcggaaagc ggcagccgga ttagccagga tgaaagcaaa acgcgacgca
    23041 gctcgacaac gcaaaaaaga atattacgca cattcaccta cccccattcc ggcctgatgc
    23101 tggctactta ccgccttatg gcggtatatc gtcacgtcca taataaatct ctgtacaaaa
    23161 aatagtctta gcgtgagata tttccgtttt ccaagcggct ccctgctcag gaaagagcag
    23221 ccataggtta tggccagagg gtaaacgatg tacagcttgt ggataatcat aagtgaagtg
    23281 caaacgatat gacgcaatta ctcttttgct tctcttttag ttacttacgt actaaaagag
    23341 aagcaaaaag aaaaacatta ctgttatccc cttttacaag tggataactg aaaaacatcg
    23401 gcgcaagcgc ctcaaaggta gcacccctcg caagcatcgg ggcattagag acaccatatc
    23461 cggtcattaa aaacgaacaa tcattcacct atcatcgctg taacatcgac ggtctacgac
    23521 cacctaccac ctaagtagct gtaagttccg tgcaattttg gcttagcagg gggctgacgc
    23581 ggttcctggc gacatgaacg tccttgatta ggggtatcgg cgcgccttag catttgtgat
    23641 tctatggaac tatatttcgc gccttacatg ccgctaacgc ggcatcttcc caccatcgag
    23701 cataacttaa ctaactgcta acaatatgat cgagaaagat caaaaaaagg ttcctgttgg
    23761 gggtcagttt ggaaatcgga aatttcggcg tttccggtgt acaaccctag gaaagtagat
    23821 ccgctcgccg caaccctagc gaccgaacgt tagtgagcga gtgggtgagg aagcggaata
    23881 gtgcccttgc tgaattgagc acatacgcga aggaattaaa ggtgagtggt ggaaaatttt
    23941 tcagttcgaa tagtgaaaaa tagttgcaat acctgcatat gcaggtataa taaaatcatc
    24001 gaaagggaaa taggttccct ttccacttag ccccataggg gcgggagatt aaaatgagtg
    24061 catttatttt tagaaccaca tttaaagcaa ctggcgatca gcaaaaagac gctattaccc
    24121 aaattgaagt tatgcaggaa caagcagtaa aagcctttgt ttcctggatg accaaccagg
    24181 agggtacaga atttttaagc actttgcagg aaatcacaac agaacttgac gcactgattg
    24241 atgcggtaga gtcccattcc cctgttttca gaaagaaagc gactgaactg gaagcgcttt
    24301 tagcccatta caaaacgttg ctgtaagtcc tcaacggggc aattgccccg ttatatggct
    24361 ctttatttaa tccaaatcta aagcaagtgg agtttaaaaa tggctcgctt ac
//
