LOCUS       NZ_CP075669             7087 bp    DNA     circular CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC2018-553 plasmid pSTEC2018_553-d,
            complete sequence.
ACCESSION   NZ_CP075669
VERSION     NZ_CP075669.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993384
            Assembly: GCF_016403675.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 7087)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: STEC2018-553
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:09:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,752
            CDSs (total)                      :: 5,624
            Genes (coding)                    :: 5,342
            CDSs (with protein)               :: 5,342
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 118 of 282
            Pseudo Genes (incomplete)         :: 157 of 282
            Pseudo Genes (internal stop)      :: 61 of 282
            Pseudo Genes (multiple problems)  :: 52 of 282
            Pseudo Genes (short protein)      :: 4 of 282
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075669.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC2018-553"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC2018_553-d"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2018"
                     /collected_by="ILS/NENT"
     gene            73..261
                     /locus_tag="JFY30_RS28380"
                     /old_locus_tag="JFY30_28380"
     CDS             73..261
                     /locus_tag="JFY30_RS28380"
                     /old_locus_tag="JFY30_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_231200041.1"
                     /translation="MDNGVTTFVKGESARRSRTTERSESVSEEAEKRLDEHFLLTHLCG
                     ISHPAWCTFHTIRTVAA"
                     /name="hypothetical protein"
     gene            complement(413..604)
                     /locus_tag="JFY30_RS28385"
                     /old_locus_tag="JFY30_28385"
     CDS             complement(413..604)
                     /locus_tag="JFY30_RS28385"
                     /old_locus_tag="JFY30_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165972.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            672..995
                     /locus_tag="JFY30_RS28390"
                     /old_locus_tag="JFY30_28390"
     CDS             672..995
                     /locus_tag="JFY30_RS28390"
                     /old_locus_tag="JFY30_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1204..>1641
                     /locus_tag="JFY30_RS28965"
                     /pseudo=""
     CDS             1204..>1641
                     /locus_tag="JFY30_RS28965"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1691..2233
                     /locus_tag="JFY30_RS28400"
                     /old_locus_tag="JFY30_28400"
     CDS             1691..2233
                     /locus_tag="JFY30_RS28400"
                     /old_locus_tag="JFY30_28400"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_231201172.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTALCVTGG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2240..2473
                     /locus_tag="JFY30_RS28405"
                     /old_locus_tag="JFY30_28405"
     CDS             2240..2473
                     /locus_tag="JFY30_RS28405"
                     /old_locus_tag="JFY30_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2625..3026
                     /locus_tag="JFY30_RS28410"
                     /old_locus_tag="JFY30_28410"
     CDS             2625..3026
                     /locus_tag="JFY30_RS28410"
                     /old_locus_tag="JFY30_28410"
                     /inference="COORDINATES: protein motif:HMM:NF016255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_052074460.1"
                     /translation="MSEQEEREQKRREYRALLEGFWPDAFNFSRPRPLKVGILAELIAD
                     AEQRGLPFDSELLTSAIKFYTRRYVYQRALTTMKERTGLDGQIAGEVTPKQREYSLKLI
                     RRFAAKDKAEKRARQEEKAAKQSGINPGA"
                     /name="ProQ/FINO family protein"
     gene            complement(3167..3589)
                     /locus_tag="JFY30_RS28415"
                     /old_locus_tag="JFY30_28415"
     CDS             complement(3167..3589)
                     /locus_tag="JFY30_RS28415"
                     /old_locus_tag="JFY30_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021539380.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3711..3989
                     /locus_tag="JFY30_RS28420"
                     /old_locus_tag="JFY30_28420"
     CDS             3711..3989
                     /locus_tag="JFY30_RS28420"
                     /old_locus_tag="JFY30_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(3981..4271)
                     /locus_tag="JFY30_RS28425"
                     /old_locus_tag="JFY30_28425"
     CDS             complement(3981..4271)
                     /locus_tag="JFY30_RS28425"
                     /old_locus_tag="JFY30_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            4515..5987
                     /gene="cta"
                     /locus_tag="JFY30_RS28430"
                     /old_locus_tag="JFY30_28430"
     CDS             4515..5987
                     /gene="cta"
                     /locus_tag="JFY30_RS28430"
                     /old_locus_tag="JFY30_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin 10"
                     /protein_id="WP_033801039.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTEVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFNSVDEALAAFEKYKNVLDKKFSKVDRDDIFNALESITYDEWAKHLEKI
                     SRALKVTGYLSFGHDVWDGTLKGLKTGDWKPLFVTLEKSAVDFGVAKIVALMFSFIVGA
                     PLGFWGIAIITGIVSSYIGDDELNKLNELLGI"
                     /name="cta"
     gene            complement(5984..6274)
                     /gene="cti"
                     /locus_tag="JFY30_RS28435"
                     /old_locus_tag="JFY30_28435"
     CDS             complement(5984..6274)
                     /gene="cti"
                     /locus_tag="JFY30_RS28435"
                     /old_locus_tag="JFY30_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 immunity protein"
                     /protein_id="WP_072023376.1"
                     /translation="MTVKYYLHNLLESLIPWLFYLLLNYKTPPFSLFIFIASIHVLLYP
                     YSKLTIFSFIQNTTKMKKEPWYSYNLFYFLYLAMAIPVGLPSFIYYSLKRN"
                     /name="cti"
     gene            6299..6430
                     /gene="ctl"
                     /locus_tag="JFY30_RS28440"
                     /old_locus_tag="JFY30_28440"
     CDS             6299..6430
                     /gene="ctl"
                     /locus_tag="JFY30_RS28440"
                     /old_locus_tag="JFY30_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 lysis protein"
                     /protein_id="WP_072023375.1"
                     /translation="MKITFLIITILSLSGCQANYISDVQGGTVAPSSSSELTGIAVQ"
                     /name="ctl"
     Typing          241..325
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          complement(6559..7084)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.669"
                     /coverage="90.0"
                     /evalue="2.02e-107"
     Typing          986..2577
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.435"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cctggggcgc ggccttttcc ggggttttcg ttttttgcgc acatgctctt ttccgggtta
       61 tccccggatt ctgtggataa cggggtcacc acctttgtga aaggagagtc cgctcgccgc
      121 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaaagcg cctggacgag
      181 cattttctcc ttacgcatct gtgcggcatt tcacacccgg catggtgcac ttttcataca
      241 atccgcactg ttgccgcata attaagccag tatacactcc gctatcgcta cgtgactggg
      301 tcatggctgc gccccgacac ccgctaaaac ccgctgacgc gccctgacgg gcttgtctgc
      361 tcccggcatc cgctcacagg ccagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      421 tttcaccgtc atccccgaag cgtgcgaggc agctgcggta aagctcgtca gcgtggtcgt
      481 gaagcgcttc gcagatgtca gcctggtcgt cggcgtccag ttcattcagt ttttccagca
      541 gggtcagcgt ctggcttttg atgaagcggg ccatgttaag ggcggttttt tcctgtttag
      601 tcacagttct gtttctccat gtgtggggag tctgtcggag gtttttaatg ataccggtga
      661 aacgcgaaag gatgctcacg atacgggtca cggatgagga acatgcgcgg ctgctggcac
      721 gttgtgaggg aaaacaactt gcgtcatgga tgcgtaaggt ctgcctgggg gcaccgccat
      781 cgaaaacgtc aggtcttccg acactggcac cgccgctgtt gcgccagttc gcttcggtcg
      841 gcaataacct gaatcagata gcccggaaga taaacagcgg acagtggtcg gggcatgacc
      901 gggttcatgt ggtggctgca ctgatggcca tcggacgtga actgagtgag ctgagggatg
      961 aggtccgtaa acagggagaa cgggatgata gttaagtttc atgccagggg aaaaggcggt
     1021 ggcagtggtc cggttgatta cctgctgggc cgggagcgta gccgggaagg ggcgagagtt
     1081 ctgcggggtg ctcccgaaga ggtgcgggaa ctcatcgatg ccacgccgtt tgcgaaaaaa
     1141 tacacctcgg gggttctgtc ttttgcagag cagaccctgc caccgggaga gcgtgaaagg
     1201 gtgatggaga gctttgagcg ggtactgatg cccggtcttg aaaaaaatca gtacagcatc
     1261 ctgtgggtgg agcaccagga caaggggcgt cttgagctga attttgtcat cccgaatatg
     1321 gagctggcga gcggaaaacg gctccagccc tattacgacc gggcggaccg tccgcgaatc
     1381 aatgcctggc agacactggt gaatcaccat tacgggctgc acgacccgaa cgcaccggag
     1441 aacaggcgga cacttgtcac cccgaataat ctgccgaaag cgaaacagga ggccgcagaa
     1501 gcgattacga ggggactgga ggctctttac catgccggag cgctaaaaac gcgtcaggat
     1561 gtcactgagg cgcttacagc ggcaggtttt gaggtggtga ggacaaccag gagcagtatc
     1621 agtatcgccg acccggaggg cgggcgtaat ctcaggctga aaggggcgct gtatgagcaa
     1681 tcttttacaa atggggacag acttcgagaa gaaactgaaa gagcgtgccg tctctacaga
     1741 gaacatgctg aacagcgagt tcaggaagct cgaagcgtct gtagacaggg aactgaactt
     1801 aaacgggcag aaaatcagag acgctatcag cgcacacaca gcagcggtga aagaacaact
     1861 ggagacgctg aacagtgccg tgaacgcgca gttcagcgcc acagagaacg aactggcccg
     1921 gcggcaggaa gagctgctct ggaagctgat caaagggcgg atactgtacc cgtcgctgac
     1981 ggcgctttgc gtcacgggcg ggatttttct ggcgtcatgg gggctgattc agtggcagga
     2041 aagccggatt gcggcgaaca tcctggctat cagggaccag gaagagacgc tggcgaaact
     2101 ccgggaaaaa acatggggtg tgacgtatca cgagggcagg aacgggaaat ttctggtgtt
     2161 gccgtccggg gtgaaggggg aaaacaactg gacggtggag aagaagaacg cagtaagact
     2221 ggtcagggag taagtgagta tgacagagct ggaaatgcac ttactgaacg ccttagagca
     2281 attgcagcag gactatacgc agcggctgaa agactgggag agcgccttca cggatttgca
     2341 gcatatgttc gagctgacac gacaggagaa cgggcaactg agggaacagt gcgacgactt
     2401 gagtcagcag gtgcagagat tagccgggca ggtggaccgc ttgagtcggt tatttatcac
     2461 gaacagcaga taagggatga acaggaaagg caggcacgcc tggaacggga gcgtgcgcgg
     2521 tcctggcatg aagagcagga acgggaagta cgggtatggc gtggtccgtc gctggggcgg
     2581 tgaatgtgta aaatagcagt gcctagctga gtgtggaggt gattttgtcc gaacaagaag
     2641 aaagagaaca gaaacgacgt gagtacaggg cgttgctgga gggattctgg cctgatgcgt
     2701 ttaatttcag ccgtccccgt ccgctgaaag tgggtattct ggctgagctg attgcagatg
     2761 cagagcagag gggcttaccg tttgattcgg aactcctgac atcggcaata aaattttata
     2821 cccgtcgtta tgtgtaccag cgtgctttaa ctacgatgaa agaacgcaca ggtcttgatg
     2881 gtcagattgc cggggaggtc acgccaaaac agcgggagta ttccctgaaa ctgatcaggc
     2941 gtttcgctgc gaaggataaa gcggaaaaaa gagcacgtca ggaagaaaaa gccgcgaaac
     3001 agtcgggaat taatccgggt gcataggtat tcatcaggtt aaaatttatc atgtgcgatc
     3061 gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgttgccgt gatcgcgcct
     3121 ggagcgtttc aacggtgcgt acaattaagg gattatggta aatcccttac tgtctgccct
     3181 cgtatccatc gagataaacc gcagaaatgg tgtctgccat tagtttcatt tttttcggcg
     3241 aaggcatatc gctgtttgtg tattttctgc cagcaaaggt tgttgtgctc cggagtgagt
     3301 gaaaaaattt ttctgagtgg aattctttta cacgttgctg tgccgtttct gctgatactc
     3361 cggcagcttt gtctttcatc cccatatccc ggaactgctg aaagaacggg agcatcatac
     3421 gtatggtgtc tgctttctgg gtgtagaagt taggatcaaa tccatcatct gaagcataaa
     3481 cataatgccg ggtatgtcgt tccaggctgg tctttggtgt gcatcctgta agagctattg
     3541 ttgctgttat cagaggtatc agaagcagcc tggcagcggc agatctcata aacatctcct
     3601 tgcgttttta atcttgatac tcagggagtt atatatccga agagtatctc ttatcatacg
     3661 tcggtttact cccaatgtga tttgatggtc aatgagtcag gaataatccc gtgaaaaaat
     3721 gtgtgccagt cctgcttttt agtgttcttt ctgcttcggt tgccagtcat gcggacagca
     3781 atgtttttca acaaaaggcc gagaagacaa aaaacggcat tgttgcggat gttaatgcca
     3841 gacagaaaat gttcagtaac agactgaaga tacaacttca gatcgatgag ttgagagcag
     3901 agctggctgg caccagagat gcccaaaaaa gagaggattt gcgtaaaaaa ctggagtctc
     3961 tggagcggca aaagaaatcc ttacgctgat gggggcgtat tttccccggt ttcttccata
     4021 acctttttac cgttcagata tttacgggcg aggcgaatgc gtcttgcctg gtataccgcc
     4081 gagtaagcct gggtgggatt cacaccatta ctgatttcac tgaccacatg acgctgatct
     4141 gccagtgtca gcgtgtaaac ctgtttctgg atgaggtcac gggctttgtc atgctcatat
     4201 ccggcagcaa caagggtgtc gagaagtcgt ttgtctgttt tgttcagttt atggcggggt
     4261 gatttgctca catcgttctc cgttttttca tgatccaaca gttttatatc tcatcctgat
     4321 ttgctccggt gttctgtttc gacgttctca atgcctgcct ccccatagtg tgatccttat
     4381 tgcaaaaatg aaaatccatg ctcttgacat ggacaatgct gagtagtagg ttttgctgta
     4441 cataaaacca gtggttatat gtacagtatg ttgtttttaa tttattgttt taattatcaa
     4501 agaggaattt ctttatggat aaagtcactg ataattctcc agatgtggag agcacagaat
     4561 ctactgaggg gtcattccca actgttgggg ttgatactgg cgatacgatt acagcgacgc
     4621 ttgcaactgg aactgaaaat gttggtggag gcggtggagc atttggtggg gccagtgaaa
     4681 gttctgctgc gatacatgca accgctaaat ggtctaccgc gcagttgaaa aaacatcagg
     4741 ctgaacaggc tgcccgtgct gctgcggctg aggcagcatt ggcaaaagcg aaatctcagc
     4801 gtgatgccct gactcaacgt ctcaaggata ttgttaatga cgctttacgt gctaatgccg
     4861 cccgtagtcc atcagtaact gaccttgctc atgccaataa tatggcaatg caggcagagg
     4921 ctgagcgttt gcgccttgcg aaggcagagc aaaaagcccg tgaagaagct gaagcagcag
     4981 aaaaagcgct ccgggaagca gaacgccaac gtgatgagat tgcccgccaa caggctgaaa
     5041 ccgcgcattt gttagcaatg gcggaggcag cagaggctga gaaaaatcga caggattctc
     5101 ttgatgaaga gcatcgggct gtggaagtgg cagagaagaa gctggctgag gctaaagctg
     5161 aactggcgaa ggccgaaagc gatgtacaga gtaagcaagc gattgtttcc agagttgcag
     5221 gggagcttga aaacgctcaa aaaagtgttg atgtgaaggt taccggattt cctggatggc
     5281 gtgatgttca gaaaaaactg gagagacaat tgcaggataa gaagaatgaa tattcgtcag
     5341 tgacgaatgc tcttaattct gctgttagca ttagagatgc taaaaaaaca gaagttcaga
     5401 atgctgagat aaaattaaaa gaagctaagg atgctcttga gaagagtcag gtaaaagact
     5461 ctgttgatac tatggttggg ttttatcaat atataaccga acaatatggg gaaaaatatt
     5521 ccagaatagc tcaggattta gctgaaaagg cgaagggtag taaatttaat agtgttgatg
     5581 aagcacttgc tgcatttgaa aagtataaaa atgtactgga taagaaattc agtaaggttg
     5641 atagggatga tatttttaat gctttagagt ctattactta tgatgagtgg gccaagcatc
     5701 tagaaaagat ctctagagct cttaaggtta ctggatattt gtctttcggg catgatgtat
     5761 gggatggtac cctaaaggga ttaaaaacag gagactggaa gcctttattt gtcactctgg
     5821 agaagagcgc ggtagatttc ggcgtggcaa aaattgtggc attaatgttt agttttattg
     5881 ttggtgcgcc tcttggcttc tggggaattg caattatcac aggtattgtt tcttcttaca
     5941 taggggatga tgagttgaac aagcttaatg aattgctagg tatttaattt ctctttagag
     6001 agtaatatat gaaacttggc aatcctactg ggattgccat tgctaggtaa agaaaataaa
     6061 aaaggttgta tgagtaccaa ggttcttttt tcatttttgt cgtgttttgt ataaaagaaa
     6121 atatagttag ttttgaatat gggtatagta atacatgtat agatgcgatg aatatgaaaa
     6181 gtgaaaatgg gggcgtctta tagtttaata acaaatagaa tagccatggg ataagtgact
     6241 ctagtaaatt gtgtaggtag tattttacgg tcattattta tgttgttata ggggtagaat
     6301 gaaaataacg tttttgataa ttacaatatt atccttgtcg ggatgtcaag caaattatat
     6361 tagtgatgta caaggaggga cggtcgctcc atcttcctct tctgaactga cagggatcgc
     6421 ggttcagtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6481 tgcttgcaaa caaaaaaacc accgctacca acggtggttt gtttgccgga tcaagatcta
     6541 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agatacccaa aacttgtcct
     6601 tctagtgtag tcaaaatcag gctgatactt caagaactct gttactaact tataaatctc
     6661 gctctgctaa cctggttacc aatggctgct gccagtggcg ttaagtcgtg tcctttccgg
     6721 gttggaccca agagaacagt taccggagaa ggcgcagcag tcgggctgaa cggggggttc
     6781 ctgcatacag cccggcccgg agcgaactgc ctaagtcgcc ccgagatacc agaagacgtg
     6841 agctatgaga aagcgccacg tgtcccgcaa gggagaaagg cggaaaaggt atccggtaat
     6901 cgatatggcg ggaacgtggg ggcgcaggag ggagcgattc accggaaacg gtgatgatct
     6961 ttaagccacg tcgggtttcg ccaccgctgg cggatttcat ggttgagccg taaggctccc
     7021 acagatgcac cgaaaaagcg tctgttttgt gaagtccgtc aggggggcgg agcctatgga
     7081 aaaagcg
//
