LOCUS       NZ_CP075662              837 bp    DNA     linear   CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC17-183 plasmid pSTEC17_183-j.
ACCESSION   NZ_CP075662
VERSION     NZ_CP075662.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993368
            Assembly: GCF_016404015.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 837)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 837)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: STEC17-183
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:06:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,332
            CDSs (total)                      :: 5,212
            Genes (coding)                    :: 4,986
            CDSs (with protein)               :: 4,986
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 99 of 226
            Pseudo Genes (incomplete)         :: 115 of 226
            Pseudo Genes (internal stop)      :: 53 of 226
            Pseudo Genes (multiple problems)  :: 39 of 226
            Pseudo Genes (short protein)      :: 4 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075662.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC17-183"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC17_183-j"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2017"
                     /collected_by="ILS/NENT"
     gene            178..456
                     /locus_tag="JFX43_RS26420"
                     /old_locus_tag="JFX43_026420"
     CDS             178..456
                     /locus_tag="JFX43_RS26420"
                     /old_locus_tag="JFX43_026420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032330704.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQINELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(448..714)
                     /locus_tag="JFX43_RS26425"
                     /old_locus_tag="JFX43_026425"
     CDS             complement(448..714)
                     /locus_tag="JFX43_RS26425"
                     /old_locus_tag="JFX43_026425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198800563.1"
                     /translation="MSGDSGGQSNTLVAAGYEHDKARDLIQKQVYTLTLADQRHVVSEI
                     SNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
ORIGIN
        1 gctattgttg ctgttatcag aggtatcaga agcagcctgg cagcggcaga tctcataaac
       61 atctccttgc gtttttaatc ttgatactca gggagttata tctccgaaga gtatctctta
      121 tcatacgtcg gtttactccc aatgtgattt gatggtcaat gagtcaggaa taatcccgtg
      181 aaaaaatgtg tgccagtcct gctttttagt gttctttctg cttcggttgc cagtcatgcg
      241 gacagcaatg tttttcaaca aaaggccgag aagacaaaaa acggcattgt tgcggatgtt
      301 aatgccagac agaaaatgtt cagtaacaga ctgaagatac aacttcagat caatgagttg
      361 agagcagagc tggctggcac cagagatgcc caaaaaagag aggatttgcg taaaaaactg
      421 gagtctctgg agcggcaaaa gaaatcctta cgctgatggg ggcgtatttt ccccggtttc
      481 ttccataacc tttttaccgt tcagatattt acgggcgagg cgaatgcgtc ttgcctggta
      541 taccgccgag taagcctggg tgggattcac accgttactg atttcactga ccacatggcg
      601 ctgatcagcc agtgtcagcg tgtaaacctg tttctggatg aggtcacggg ctttgtcatg
      661 ctcatatccg gcagcaacaa gggtgttaga ctggccccct gaatctccag acaaccaata
      721 tcacttaaat aagtgatagt cttaatacta gtttttagac tagtcattgg agaacagatg
      781 attgatgtct tagggccgga gaaacgcaga cggcgtacca cacaggaaaa gatcgca
//
