LOCUS       NZ_CP075659             3137 bp    DNA     linear   CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC17-183 plasmid pSTEC17_183-g.
ACCESSION   NZ_CP075659
VERSION     NZ_CP075659.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993368
            Assembly: GCF_016404015.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 3137)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: STEC17-183
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:06:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,332
            CDSs (total)                      :: 5,212
            Genes (coding)                    :: 4,986
            CDSs (with protein)               :: 4,986
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 99 of 226
            Pseudo Genes (incomplete)         :: 115 of 226
            Pseudo Genes (internal stop)      :: 53 of 226
            Pseudo Genes (multiple problems)  :: 39 of 226
            Pseudo Genes (short protein)      :: 4 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075659.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC17-183"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC17_183-g"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2017"
                     /collected_by="ILS/NENT"
     gene            complement(<1..290)
                     /locus_tag="JFX43_RS26390"
                     /old_locus_tag="JFX43_026390"
     CDS             complement(<1..290)
                     /locus_tag="JFX43_RS26390"
                     /old_locus_tag="JFX43_026390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_215735942.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEA"
                     /name="MobA/MobL family protein"
     gene            466..861
                     /locus_tag="JFX43_RS26395"
                     /old_locus_tag="JFX43_026395"
     CDS             466..861
                     /locus_tag="JFX43_RS26395"
                     /old_locus_tag="JFX43_026395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222910.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYPKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     gene            complement(1968..2498)
                     /locus_tag="JFX43_RS26400"
                     /old_locus_tag="JFX43_026400"
     CDS             complement(1968..2498)
                     /locus_tag="JFX43_RS26400"
                     /old_locus_tag="JFX43_026400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181206108.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNKDIATIH
                     HNVVKEYNTLNKYSLKWFIGVTVSFLVMFSLLGWFTKTLIDRNYTATEVVYQKIVDIEK
                     KLDKPATGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2501..>3137)
                     /locus_tag="JFX43_RS26405"
                     /old_locus_tag="JFX43_026405"
     CDS             complement(2501..>3137)
                     /locus_tag="JFX43_RS26405"
                     /old_locus_tag="JFX43_026405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_175176013.1"
                     /translation="GERHAWSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYF
                     RRYNARYPERGGAKKDSGSLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTL
                     KEQGIERRPEVHLGFEAAGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTP
                     VKQHELVRRLQHDVAREHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     Typing          complement(4..290)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.917"
                     /coverage="99.0"
                     /evalue="5.19e-55"
     Typing          complement(2505..3136)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.104"
                     /coverage="99.0"
                     /evalue="6.49e-141"
ORIGIN
        1 gcttcctgac gtacaaaatc ctgcaccagt tcaaggcgct gttccgctgt cagttcacgg
       61 ggtaatgcaa tttcaagttc ccgataggtc gaaccgttcg cacgttcaaa ctggtcagcc
      121 gcctgccaga aatgtgaggg attatctcgc gcccattccg gcatgtttcc gtgtgcggaa
      181 tattccaaat cctgacggtc gcggtatttc tctttgcgct cgatgtaatc ggcgtggttt
      241 gcagcctgcc ccttgccacc aaatttaacg ctgaggtgat aggttgccac ggtttcagcc
      301 ctccgcagga gcgcactgtt tctcgacgaa ggagagaaac atataagtgc gcttccttct
      361 aattttacga attagcacgg aagatacccc ctgcacccac cgaccgcaag cgatatactg
      421 cacaacccca acacaccgca aaaaacacca gggagcatcc caaacatgcg catcagcaac
      481 atcgactggt tgaagaaacg aatcggcttt atcaggaagc tcggagaaca gaccgcacga
      541 cagaggcaga tcattgacct gctcgataac gaagccggac taacagaaca ggagcgcaaa
      601 ctactccacg tcctggcaac cgcagaaaaa aacgagctac aggcacagga gaacgcacgc
      661 aaacaggcca accaaaaacg gatggagggg aaaacacaac gacgggaacg caatcaccgc
      721 ctgtttcttg cggcggggtt actgatcgag gccggactag tggacacaaa aacaggcgaa
      781 ctgagatacc caaaagacaa tatattacag aaacttaagg caatcaggct cgatcttgaa
      841 acaagcccag atcatcatta attccgctgc gtggtggtgc gtttgcctgc cggtgttcaa
      901 tgttttcgat ttcgcgacgc gctacgcgcg gatctggcac aggcgcgtaa gtgcacaatg
      961 cttgcctctg tgactcttcc gcttcctcgc ccactcgctc actacgttcg gtcgctgggg
     1021 ctgcggcgag cggtgggatc actctcgttg tgatgcagat ccgttttttt ttttgcactg
     1081 gagttatcag gagtagcggt gcacatggga tcgtgcgata tgctggatgg tggaaggtcg
     1141 gagggaacgg caacaactca cccctcccag tgaagggacg ggtagtttgc cgctctcgat
     1201 atgccggacg gagccaaggc catcgatcac ggggcgcgtc agtggaagaa cccgcgcctg
     1261 cgtgacgtat gcgtcttcgc cgcaccatct cacccgtcac gccaccacat accgggaact
     1321 gccgccaagg tctaactcaa ccaatctagc cgcgctacat acggtttttg tggcaactca
     1381 tcaggtgtcc gctgtcgatg aaactctctg cgccggtagc ggaactctca cgcgtgcggt
     1441 cgccgtcgtc acagctcccc caaccgcaaa cgattctcgc aaatacgcaa gtatctgcaa
     1501 aaacaggttt ttttcggggt tcctgggaga ctgtgctgtt gaaaaattct tgaagaatac
     1561 agaaggctca tgcaaactga atgaactggt ttctgtcgct cctaacaaca gactccacag
     1621 gaacattgag gaggtcgaaa acctcaatga acccgaagaa gctcgcctaa tggcgggctt
     1681 ttttcgttct acgcccgccg acgaaagatc gcaagtgcaa taacgagatt tttcacgtct
     1741 ttacgctcaa aaagtaaaaa aaccatgttt tcccctggct gaatccccaa aaacgcgtga
     1801 aatgcgtttt caggatgttg catagctatg cacaaatacg gcacacgatt tgtttcgctc
     1861 gccccagccg tcgaacgggt caaaatggca aactttctca tcagaaaacg gtgaacggga
     1921 cgcggaattt tttcgcgtcc cgtgaggcgt tggacaagcg aagcgcgtca gtttttccgg
     1981 ctcttccctg ttgctggttt gtccagtttc ttttcaatat caacaatttt ctgatacacg
     2041 acctccgtcg cggtgtagtt cctgtcgatc aacgttttgg taaaccagcc aagcaacgag
     2101 aacatgacca gaaaactgac cgtaacaccg ataaaccatt tcagactgta tttattgagc
     2161 gtgttgtatt ctttaacaac attgtgatga attgtcgcaa tgtccttgtt cagggcatcc
     2221 gcgtttgcct tcatgtttgc gactgactct tccagccgct gaaaatcctg gctgatgcgc
     2281 tcacctatca gccctgcaac atccagcttc tgcacctcgt ttctgacttc atccctgacc
     2341 tgttttgcca caccgtcaga aatatcccga tatccgcgaa gtgtttccct tgccacagcc
     2401 agagaatcag caacagcttt ctgttgctcc attgcggtat gttgcagatg ctccaccgat
     2461 aacaggagcg tgagtaattt ttcttctgtg ctggccatgt tcagaatccc atagaaaatc
     2521 cacgtgacgg tgatttaacc cgttcaggtt cagttcggtt ctgtctgctc actctgtctc
     2581 tcatgtcaaa gccatgctcc cgcgcaacat catgttgcag ccttctgacc agttcatgtt
     2641 gcttaaccgg agtgtccagc cattccctga tgtcatgccc gaccgttcgc gcctgttcaa
     2701 gatggcggga atagtccggt tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat
     2761 caagccgtcc ggcagcttca aaacccagat gcacttcggg acgtcgttca atgccctgtt
     2821 ctttcagcgt ccggcagtca atgaagcccc tttcaaagcc atgctttctc atatgttcgt
     2881 tgtgcttcac ctcccagcgt tttcgcaatt cccgcaactg ctctttttgc tgagtgagag
     2941 tcaggcttcc ggaatccttt ttcgcacctc cccgctcggg atacctggcg ttatagcgcc
     3001 ggaaatactg ttccggtgta cgttcaatgc catcattcac acgctggctc atcatgatat
     3061 gcgcatgagg ctgttcgccg ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc
     3121 aggcgtgccg ttcgcca
//
