LOCUS       NZ_CP075658             3563 bp    DNA     linear   CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC17-183 plasmid pSTEC17_183-f.
ACCESSION   NZ_CP075658
VERSION     NZ_CP075658.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993368
            Assembly: GCF_016404015.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 3563)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: STEC17-183
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:06:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,332
            CDSs (total)                      :: 5,212
            Genes (coding)                    :: 4,986
            CDSs (with protein)               :: 4,986
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 99 of 226
            Pseudo Genes (incomplete)         :: 115 of 226
            Pseudo Genes (internal stop)      :: 53 of 226
            Pseudo Genes (multiple problems)  :: 39 of 226
            Pseudo Genes (short protein)      :: 4 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075658.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC17-183"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC17_183-f"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2017"
                     /collected_by="ILS/NENT"
     gene            479..706
                     /locus_tag="JFX43_RS26375"
                     /old_locus_tag="JFX43_026375"
     CDS             479..706
                     /locus_tag="JFX43_RS26375"
                     /old_locus_tag="JFX43_026375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024194140.1"
                     /translation="MSVDVIKQIQSIQDKGIIIYSKYRANEFDRDDVYRESYTLIIEFN
                     HLIAEHAVHDEKLINHTICILNELRSIAIN"
                     /name="hypothetical protein"
     gene            1341..1778
                     /locus_tag="JFX43_RS26380"
                     /old_locus_tag="JFX43_026380"
     CDS             1341..1778
                     /locus_tag="JFX43_RS26380"
                     /old_locus_tag="JFX43_026380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_136763429.1"
                     /translation="MAVAASARNALKIIRCTANKKLSSTKNHVKTIASTFLSPSITVKH
                     SASVGKLLTSIIQFLIDNNSGDIPSFEGLFLPTHHKQHPYHRQNRAQNVSDGLNQPKHI
                     KPLPDAKNRVRKRLSAFLESKKLSAVDFHCRDPETRSIIAA"
                     /name="hypothetical protein"
     gene            complement(1761..2759)
                     /locus_tag="JFX43_RS26385"
                     /old_locus_tag="JFX43_026385"
     CDS             complement(1761..2759)
                     /locus_tag="JFX43_RS26385"
                     /old_locus_tag="JFX43_026385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_016237859.1"
                     /translation="MKNHELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGER
                     VGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPKIV
                     AEYPGARWLFLTLTVRNCSIDALGETLTAMNAGFLNLKRRKEFRGVLGWVRTTEVTRGK
                     DGSAHPHFHTLLMVSPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRKPKDD
                     ESLPCAVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGALKNVLQ
                     LERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
                     /name="protein rep"
     Typing          complement(1117..1194)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="87.0"
                     /evalue="1.22e-26"
     Typing          1853..2042
                     /name="Col(MP18)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.476"
                     /coverage="99.0"
                     /evalue="1.71e-93"
ORIGIN
        1 catgatgcgc ttcattatgc caaaggcgaa aatatagact ggtgtatagc cagagctgaa
       61 tactgtaaaa agacaggccg cttttaattc tggcgggatt tcaaaggggc gtcccctttg
      121 gcacgtcgac gtatcaaaat gcgccagcat tttgtataca gtcggtaacg tgcctccaga
      181 atttcagcaa ccgcggactc ttaacaatca ataatcacta atacgcacta atacgcatta
      241 ataatcacta atgagcacta ataagcacta aatattgggt ctgcgccaaa cgaactcacc
      301 aacggatcgc atgtagtaga cgctacataa aaaatcctga aatggaggat tcgtcataaa
      361 gagattcctg tttttagcgt tctgatgcag gatagggcaa acaccgcaaa atgacgtctt
      421 tggtatgttt tgtgatgttt gcttctggca tctgttatct gttttattgg gtgtttttat
      481 gagtgttgat gtaattaaac aaattcagag catacaggac aaaggaatta ttatttattc
      541 aaaatacaga gcaaatgaat ttgaccggga tgatgtatac agggagagtt acaccctgat
      601 tattgagttt aatcatctta ttgctgaaca tgccgttcat gatgaaaaac tgattaatca
      661 tactatctgt attctgaatg aattacgaag catagcgata aattaaaaaa tccccctgga
      721 ttacaggggg aagccatcag agtttgagta atgaaaaata ttattttgga gagagcacca
      781 caaatgcgct cttttccaat ctgccgtaac catcagcaaa agacaatcag gacaggcgaa
      841 aaaatccccc tatatggcaa gggggtgtca atgaggacat ggcaattgtg agttttgtga
      901 gttttgtgtg ttttatgtgt tgcagtttgc aacgtcagaa atttgccgta gttcgtcatg
      961 agaggcaaca aaaaaatccc cccccggaac tggtggaggc cagggccaga ggggtagcaa
     1021 atggctaaat aattttcgta gttttgatat tcccatcgtt gcaaccgcaa cggggtgaat
     1081 ttgccgcagt tgtacgtgaa aaacaatcct gatttcggga caggatgtgt aatcgtataa
     1141 ccgcgcacgc acggatatac gattaccacc tagtcagggc tttgcccgac accctcgaca
     1201 cagttcccgc aatacgatag aacatcaggg cgcgtttccg ggtaaacgca tgttaatgac
     1261 cgggaattca ttgattaggg accggagacg cgctacaaaa ctcctgcgcg tttctggcat
     1321 caactgacca tggggatggt ttggcagtag cagcaagcgc cagaaacgcg ctcaaaatca
     1381 tcagatgtac agcgaacaaa aaattgtcat ctacaaaaaa tcacgttaaa acaatcgcga
     1441 gcaccttcct gtctccttca attacggtca aacattctgc atctgtcggg aagttgctca
     1501 cttccataat tcaatttctc attgacaata attccggtga tatcccctct tttgaggggc
     1561 tttttttacc cacacaccac aaacagcatc catatcatcg tcaaaatcgc gctcaaaacg
     1621 tctctgacgg cctgaatcaa ccaaaacata taaaaccatt gccagatgca aaaaatcgcg
     1681 ttagaaagcg tctgagtgcg tttctggaga gcaaaaagct gtcagccgtg gatttccatt
     1741 gcagagaccc agaaacgcgc tcaatcatcg ctgcttagcc gacgtttatc tttcgcccga
     1801 tcgcgctggt attttttgac aaccggcacc cacgaaaacg ccagacggga accgtcatct
     1861 gtaccctctg ccatgccatc cgccatgatt aaatcctcgt tcgtttcccg ctccagttgc
     1921 agaacgttct taagcgctcc ccccgtagca acaaaacgac gcttgtgtac ctgccgtgtt
     1981 agttcgagga accattcagg atcagccagc atatcagcag gctttgtgga atatttcaga
     2041 gtctcaacca ccgcgccgcg cagctgctcc gcaacagcac aaggtaaaga ttcatcgtct
     2101 ttcggtttcc gtgacttcac caccctgata tccacattcg ggtcatacgc cacccgcaga
     2161 caatcacgcc acagttccac ccaacgggca tgtttcacat actcacggcc ggacaacatc
     2221 gagggggaaa ccatcagcag agtgtgaaaa tggggatgtg cgctaccatc cttgccgcgc
     2281 gttacttccg tggtacgaac ccagcccagc acaccacgaa actccttgcg acgcttgaga
     2341 ttcaaaaaac ccgcattcat cgcggtcaaa gtctcaccaa gcgcatcaat ggaacagttg
     2401 cgcaccgtca gcgtgagaaa caaccagcgc gcgcctggat attccgccac aatctttggc
     2461 agggactgat agaaacgggc ttgccacatc atggagcgcc tccactggca gatcggacag
     2521 tgccggacac gacagaaccg cgcatcacgc aaccgcagac gcgtttctcc ggtctctgtg
     2581 tttgttgacc acccaaaccg caaaatacca ctacaagccc cgacacgttc gccgtagtgc
     2641 tcaaatccgg cagcacgcag ataaataccg cccacgtcgt cggaaaagct ccggcgctca
     2701 tcccacggtt tatcctccgg ggaataatcc gtcagatttt taagttcatg atttttcatg
     2761 tctgccgttc cctagtgagc gacagacgaa attacgtgtt atcattcctg tatcacctgt
     2821 gttgtcgtgt caccaattcg aattcctaca ggtggtcaca cccccgaacg acgcccatcg
     2881 ttgcgggggt cgcttttaat gcgaccactt ccgacaaaat actctcaaac ctattttcac
     2941 gcaaatccac gttcaggcca gtcgtgacaa ggcctagagc atgatttcga gttgtcgatt
     3001 tatcccttaa gtatatatac aaggtggcga ccacctaaaa acgcatcaat ccacgagctc
     3061 ccactggtag cacccagcac acgaaagaac atccgtaata acaccactcg gcgtatgaat
     3121 ctcagccaca accctttgat taccgtagta aggcgcataa cccgaaagct tcccgcgagt
     3181 cttcccacca atggatttaa taaccgcaac agttctcccc catgatgtcc gccgaacctt
     3241 aaatccgact ctgttgcaat tttcctcgta tagctgatag atatttttca attcccatcc
     3301 tcctaaaaac ccgctttgca ggtggttggc ctcactgcgt gacaccaacc accatcacca
     3361 gtattttttc tcgtaccgtc gtcaatcaca aaatcggaac ccacgccgga ggcgcgggac
     3421 cccacgattt tctgtttgac gagtcagaaa acggcgaacg ctgacagatt tcaatctggc
     3481 tgcgtgagac gttggcgggg tgtcggggcg cagtcctgac caggcggcat ttgtctgatt
     3541 gcgtgtgcgc ggtccgacaa ata
//
