LOCUS       NZ_CP075657             6744 bp    DNA     circular CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC17-183 plasmid pSTEC17_183-e, complete
            sequence.
ACCESSION   NZ_CP075657
VERSION     NZ_CP075657.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993368
            Assembly: GCF_016404015.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 6744)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: STEC17-183
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:06:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,332
            CDSs (total)                      :: 5,212
            Genes (coding)                    :: 4,986
            CDSs (with protein)               :: 4,986
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 99 of 226
            Pseudo Genes (incomplete)         :: 115 of 226
            Pseudo Genes (internal stop)      :: 53 of 226
            Pseudo Genes (multiple problems)  :: 39 of 226
            Pseudo Genes (short protein)      :: 4 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075657.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC17-183"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC17_183-e"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2017"
                     /collected_by="ILS/NENT"
     gene            1..915
                     /locus_tag="JFX43_RS26345"
                     /old_locus_tag="JFX43_026345"
     CDS             1..915
                     /locus_tag="JFX43_RS26345"
                     /old_locus_tag="JFX43_026345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_198800543.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCYLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRALGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVATSARTL
                     KPWEKLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1140..1406)
                     /locus_tag="JFX43_RS26350"
                     /old_locus_tag="JFX43_026350"
     CDS             complement(1140..1406)
                     /locus_tag="JFX43_RS26350"
                     /old_locus_tag="JFX43_026350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091750.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
                     /name="hypothetical protein"
     gene            1585..3804
                     /gene="mobQ"
                     /locus_tag="JFX43_RS26355"
                     /old_locus_tag="JFX43_026355"
     CDS             1585..3804
                     /gene="mobQ"
                     /locus_tag="JFX43_RS26355"
                     /old_locus_tag="JFX43_026355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_198800544.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPENAPSWCADRSVLWNAVEKAEQRRDSQLAREIELAIPREISRDAARET
                     VLAFVRENFVSQGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKSACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRKVQETQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            4037..5782
                     /gene="col"
                     /locus_tag="JFX43_RS26360"
                     /old_locus_tag="JFX43_026360"
     CDS             4037..5782
                     /gene="col"
                     /locus_tag="JFX43_RS26360"
                     /old_locus_tag="JFX43_026360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_000012961.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKADADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEVSKDPDLSKQ
                     FKGSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNIRVTTPKRHID
                     IHRGK"
                     /name="col"
     gene            5785..6045
                     /locus_tag="JFX43_RS26365"
                     /old_locus_tag="JFX43_026365"
     CDS             5785..6045
                     /locus_tag="JFX43_RS26365"
                     /old_locus_tag="JFX43_026365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420693.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            6107..6250
                     /locus_tag="JFX43_RS26370"
                     /old_locus_tag="JFX43_026370"
     CDS             6107..6250
                     /locus_tag="JFX43_RS26370"
                     /old_locus_tag="JFX43_026370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6744
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1411..1584)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          complement(1585..1943)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.389"
                     /coverage="100.0"
                     /evalue="3.99e-167"
     Typing          2..915
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.557"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1586..3801
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.782"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgaagaa aatttacccc accgtcccta tcacacggat
       61 gatctcgctt ttggtcttcg catctccggc aaagggcgtg cgcttcttgc gcggtacatc
      121 cagcagaacc agcctcatgc gcagttctgg ctggtttttg acgttgatcg cgagggggct
      181 gcgattgact ggagcgaccg gaacgccccc gcgccgaaca tcaccgtaaa aaatcccgtg
      241 aacggtcatg ctcacctgct ctatgcactc aacatcgccg tgagaaccgc gcctgatgcg
      301 tcggttaagg cactgaagta cgctgccgcg atagagcgtg cgctgtgtga gaaacttggt
      361 gcagatgtga actacagcgg actgatttgc aaaaatccgt tccacctgga atggctggtg
      421 atggagtggc gcgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagcgc gccgtagcat cgataaacat tacgggatgg ggcgaaactg ctacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggcctgt attctcacaa
      601 tggcttgatg ccgtgatcca gcgtgtcgaa atgtacaacg catctcttcc cgttccgctt
      661 tctccggctg aatgtcgggc tcttggcaag agcatagcga aatacacgca caggaacttt
      721 acaccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat tcaggctaca
      781 cgcggtcgca agggcggttc taagtctaag cgcggcacag tagctacatc agcacgcacg
      841 ctgaaacctt gggagaaatt agggatcagt cgcgcctggt attaccaact gaaaaaacga
      901 ggtctcgtag agtagaccaa ataagcctat atcagataac agcgcctttt tggcgttttt
      961 ttgagcagct tggtttgttg ctatttccct cgttgaatcc cgcaatggcg cggctttccg
     1021 catgattgag gtggtagcgc tcgccgcagt ctcatgaccg agcgtagcga gcgaatgagc
     1081 gaggaagcgc aaaggtgtcg gtggtgcatg tggcacttac gcgccggggt ttctccgttc
     1141 tacggtttcg caggtggttt gggtagcttc tccagctcgt taatcagcgg ttgtatccgg
     1201 ttcacatcgg cctgtcttgt gacttccttt cgcaggaact ggagcaggaa cgcacgcagt
     1261 tgcgcttctt ccggcctccg taccctcgcc agcatggcag cccccacaat gactttttgc
     1321 gccgtgtcca ggctccggct cttcgccttc aggcgctgta atctggcctc agcttcggca
     1381 atcttctgtt cgagtgttct gctcatttcg tgactccgtg cgcggtgaaa aatcgcattt
     1441 tagcgcgtca ctggtagttg caaaactgaa ctggcataat gcacggcaca tcacgaagtg
     1501 cgcacttata caatctccac tccgtttcga ttgtgtgcgc cctgcggggc tgacagaaaa
     1561 cggcaaaaag gcattacggc agaaatggcg atttatcatc tcagcatgaa aatcatttcg
     1621 cgaaaaaacg gctacagtgc cgttgcttct gctgcctacc gttccggctc tgtcataccc
     1681 gatgaccgta ccggattaac ccacgattac acccgtaaac gcggcgttga tgatgcggtc
     1741 attctcaccc ctgagaatgc accgtcatgg tgtgctgacc gttccgttct ctggaatgcg
     1801 gtcgagaaag ccgaacagcg ccgggactcc cagctggcaa gggagattga acttgccatt
     1861 ccccgagaga tttcccgcga tgccgcacgg gaaaccgttc tcgcttttgt cagggaaaac
     1921 tttgtcagcc agggcatgat tgccgatgtg gcgttccatc acatggaccg gactaatccc
     1981 catgcgcaca tcatgctgac cacaagagct gtcggggaaa cgggattcgc aggaaaggtc
     2041 agggactgga acgaccgggc actcgccgag acgtggcgcg catcatgggc tgaccatgcg
     2101 aacagagcgc ttgctaacgc cggctaccag gaagagatag accatcgttc atacgagcgt
     2161 cagggactgg agaaagcgcc gggccttcac ctcggaaagt ctgcctgtgc gatggaaaaa
     2221 cggggcatgg aaacagaacg cggtgagcag aaccgcctga ttaacagcct taacctggaa
     2281 atacaggttt cccgcacgca gctggcgctc aggaaggttc aggaaacgca gcgtaagcgg
     2341 gaactcagcg atgccgcacg tcgcgcagcg gaagccctta acctgaccat tcccgctgcg
     2401 aatgcctcag cggataccct gcgggaattc attgccacgc tgccgcagga atgcgggaac
     2461 gcgtgggaga tgaccccgga gttcctggcg atgagcggga aggtgaacga catcgaacgt
     2521 gaggggaatg cgctgctgaa agagcaggcc attctcgaaa aggagatgac cggactcaaa
     2581 aaagcacgcc ctgtcgcgtc ccttctgtca gagattcccc tgatgacatg ggctgaaccg
     2641 gaataccgca aaagacaact ccgtttctgg aaactcggga aacagattga atctcttcgc
     2701 cgcacctaca gggccgtgaa agaacgggac attcccgccc gtcgtcaggc ctttgaaacg
     2761 cagtggaata cgtggattgc gccgggaatg gcagagctga aagaaaaact gtcagcacgg
     2821 gaagcggagc ggcgcaggga ggaagccgaa gcggaagcgc gccggaagga acaggagcat
     2881 gaggcgcggc tgaaacgtca tgataaccac cgtctgagcc gtgaaacggc attagtcggg
     2941 gttattacgg agctggggcg tgccagagag ccgggaacgg gcaggataac ccgctacatg
     3001 atgttgagta acagagccgg agaattcacg gtatggggtg atgagctggc gcattacccc
     3061 cagagtgttc atgacccggt gaatgtttac ctgtcgccag gcggggctgt gatggtctcg
     3121 gatatacgcg agggaatgcc agaatctcat gagacgatgg cgcggcctga gcgtgtgaga
     3181 atgtattccg gtgcgacggt ccggcatgta ctggaacaga tgcgccaggg gtggccctct
     3241 tacggttttc cggcgctgcc gcatcactgg ccggataatt tttatttcag cgacgaccgc
     3301 aggcccgtag cctctccgct gccgtcagcg caccgggtgg atatcaccgc tgatgctgta
     3361 ccggaacaac tcatgcctgt tgtattttcg agagagggaa acagcaggac gctgaatctg
     3421 ttgttatgca aagggccgga ggaaatgctt gtcgggtttg ttcgccggga aggagggttt
     3481 tgccccgttc ttgtgcttcc gtcgccggat tacagtcatc tgatagtcag caccatcacg
     3541 gagaacgggg tatgcctggc aggttacgga gaagctataa accatgatga ggatactccg
     3601 tacccaccgg aaccgcacct gatgcagttc cggctcaaag gccatcatga caggcttttg
     3661 gctgctgtcc acaaaccgga agagatgccg gattatctct tccgtcaact cggttttaat
     3721 cagacctggc atgagtggaa gcgggacgaa cagcacaggc aacaacaacg ccgccccggg
     3781 cacttccggg gcatgagtat gtgatatccg gggctgcacc ccggaccccg ccaacacatc
     3841 acgggccaca aaattttttg tggcccgctc tgcgttttct aagtgttatc cctcctgatt
     3901 tctaaaaaat tttccgcctg aacttgacag aaaaaacgat gacgagtact ttttgatctg
     3961 tacataaaac cagtggtttt atgtacagta ttaatcatgt aattaattgt tttaacgctt
     4021 aaaagaggga atttttatga gcggtggcga tggacgcggc cataacacgg gcgcgcatag
     4081 cacaagtggt aacattaatg gtggcccgac cgggcttggt gtaggtggtg gtgcttctga
     4141 tggctccgga tggagttcgg aaaataaccc gtggggtggt ggttccggta gcggcattca
     4201 ctggggtggt ggttccggtc atggtaatgg cggggggaat ggtaattccg gtggtggttc
     4261 gggaacaggc ggtaatctgt cagcagtagc tgcgccagtg gcatttggtt ttccggcact
     4321 ttccactcct ggagctggcg gtctggcggt cagtatttca gcgggagcat tatcggcagc
     4381 tattgctgat attatggctg ccctgaaagg accgtttaaa tttggtcttt ggggggtggc
     4441 tttatatggt gtactgccat cacaaatagc gaaagatgac cccaatatga tgtcaaagat
     4501 tgtgacgtca ttacccgcag atgatattac tgaatcacct gtcagttcat tacctctcga
     4561 taaggcaaca gtaaacgtaa atgttcgtgt tgttgatgat gtaaaagacg aacgacagaa
     4621 tatttcggtt gtttcaggtg ttccgatgag tgttccggtg gttgatgcaa aacctaccga
     4681 acgtccaggt gtttttacgg catcaattcc aggtgcacct gttctgaata tttcagttaa
     4741 taacagtacg ccagcagtac agacattaag cccaggtgtt acaaataata ctgataagga
     4801 tgttcgcccg gcaggattta ctcagggtgg taataccagg gatgcagtta ttcgattccc
     4861 gaaggacagc ggtcataatg ccgtatatgt ttcagtgagt gatgttctta gtcctgacca
     4921 ggtaaaacaa cgtcaggatg aagaaaatcg ccgtcagcag gaatgggatg ctacgcatcc
     4981 ggttgaagcg gctgagcgaa attatgaacg cgcgcgtgca gagctgaatc aggcaaatga
     5041 agatgttgcc agaaatcagg agcgacaggc caaagctgtt caggtttata attcgcgtaa
     5101 aagcgaactt gatgcagcga ataaaactct tgctgatgca atagctgaaa taaaacaatt
     5161 tgaacgattt gcacatgacc caatggctgg cggtcacaga atgtggcaaa tggccgggct
     5221 taaagctcag cgggcgcaga cggatgtaaa taataagcag gctgcatttg atgctgctgc
     5281 aaaagagaag gcagatgctg atgctgcatt aagtgccgcg caggagcgcc gcaaacagaa
     5341 ggaaaataaa gaaaaggacg ctaaggataa attagataag gagagtaaac ggaataagcc
     5401 agggaaggcg acaggtaaag gtaaaccagt tggtgataaa tggctggatg atgcaggtaa
     5461 agattcagga gcgccaattc cagatcgcat tgctgataag ttgcgtgata aagaatttaa
     5521 aaactttgac gatttccgga agaaattctg ggaagaagtg tcaaaagatc ccgatcttag
     5581 taagcaattt aaaggcagta ataagacgaa cattcaaaag ggaaaagcac cttttgcaag
     5641 gaagaaagac caagtaggtg gtagggaacg ctttgaatta catcatgata aaccaatcag
     5701 tcaggatggt ggtgtctatg atatgaataa tatcagagtg accacaccta agcgacatat
     5761 tgatattcat cggggtaagt aaaaatggaa ctgaaacata gtattagtga ttataccgag
     5821 gctgaatttc tggagtttgt aaaaaaaata tgtagagctg aaggtgctac tgaagaggat
     5881 gacaataaat tagtgagaga gtttgagcga ttaactgagc acccagatgg ttcagatctg
     5941 atttattatc ctcgcgatga cagggaagat agtcctgaag ggattgtcaa ggaaattaaa
     6001 gaatggcgag ctgctaacgg taagtcagga tttaaacagg gctgaaatat gaatgccggt
     6061 tgtttatgga tgaatggctg gcattctttc acaacaagga gtcgttatga aaaaaataac
     6121 agggattatt ttattgcttc ttgcagtcat tattctgtct gcatgtcagg caaactatat
     6181 ccgggatgtt cagggcggga ccgtatctcc gtcatcaaca gctgaagtga ccggattagc
     6241 aacgcagtaa cccgaaatcc tctttgacaa aaacaaagcg tgtcaggctg attctgatgc
     6301 gctttttttt gaaatgtcac aaaaattcca tgtgggagat gggatctaaa atccccgtgc
     6361 agaactttcc atccaggggg agaaaacttg tcgttttgag ccgttcggtg ttcagaacgc
     6421 acgaaaccga tcgcgcatcg ctttcgtgca tagttatgca acgcgctaaa aacgattctg
     6481 acgcattttt tatgtttacc ctggtgtttc cttgtctttt tgcgtttttt gcgtcagaaa
     6541 gcgattgagg gcgttttaag gggtgcgtac aacgggagtt atggtaaatg gatcgggttt
     6601 tcgggaaggt tcgacaggat ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc
     6661 ccctgaaatt cgtgtctctc cgccaagatt gtcacgagaa tacaggggcg aggtcggtcg
     6721 ataacctgga aggaatcgta acac
//
