LOCUS       NZ_CP075656             7684 bp    DNA     circular CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC17-183 plasmid pSTEC17_183-d, complete
            sequence.
ACCESSION   NZ_CP075656
VERSION     NZ_CP075656.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993368
            Assembly: GCF_016404015.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 7684)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: STEC17-183
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:06:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,332
            CDSs (total)                      :: 5,212
            Genes (coding)                    :: 4,986
            CDSs (with protein)               :: 4,986
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 99 of 226
            Pseudo Genes (incomplete)         :: 115 of 226
            Pseudo Genes (internal stop)      :: 53 of 226
            Pseudo Genes (multiple problems)  :: 39 of 226
            Pseudo Genes (short protein)      :: 4 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075656.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC17-183"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC17_183-d"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2017"
                     /collected_by="ILS/NENT"
     gene            complement(244..438)
                     /locus_tag="JFX43_RS26300"
                     /old_locus_tag="JFX43_026300"
     CDS             complement(244..438)
                     /locus_tag="JFX43_RS26300"
                     /old_locus_tag="JFX43_026300"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="WP_001367294.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAKG
                     ENIGWCIARAEYCKKTGRF"
                     /name="ANR family transcriptional regulator"
     gene            1058..1255
                     /locus_tag="JFX43_RS26305"
                     /old_locus_tag="JFX43_026305"
     CDS             1058..1255
                     /locus_tag="JFX43_RS26305"
                     /old_locus_tag="JFX43_026305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_028132815.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQTHFEAQYGIEQP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1920..2063
                     /locus_tag="JFX43_RS26310"
                     /old_locus_tag="JFX43_026310"
     CDS             1920..2063
                     /locus_tag="JFX43_RS26310"
                     /old_locus_tag="JFX43_026310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(2262..2393)
                     /locus_tag="JFX43_RS26315"
                     /old_locus_tag="JFX43_026315"
     CDS             complement(2262..2393)
                     /locus_tag="JFX43_RS26315"
                     /old_locus_tag="JFX43_026315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001368419.1"
                     /translation="MKIVIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            2417..2707
                     /gene="cki"
                     /locus_tag="JFX43_RS26320"
                     /old_locus_tag="JFX43_026320"
     CDS             2417..2707
                     /gene="cki"
                     /locus_tag="JFX43_RS26320"
                     /old_locus_tag="JFX43_026320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_072149383.1"
                     /translation="MYLKYYLHNLPESLIPWILILIFNDNDNTPLLFVFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(2704..4350)
                     /gene="cka"
                     /locus_tag="JFX43_RS26325"
                     /old_locus_tag="JFX43_026325"
     CDS             complement(2704..4350)
                     /gene="cka"
                     /locus_tag="JFX43_RS26325"
                     /old_locus_tag="JFX43_026325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_165371048.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVVEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGTDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="cka"
     gene            4591..4881
                     /locus_tag="JFX43_RS26330"
                     /old_locus_tag="JFX43_026330"
     CDS             4591..4881
                     /locus_tag="JFX43_RS26330"
                     /old_locus_tag="JFX43_026330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032330705.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEYDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(4873..5151)
                     /locus_tag="JFX43_RS26335"
                     /old_locus_tag="JFX43_026335"
     CDS             complement(4873..5151)
                     /locus_tag="JFX43_RS26335"
                     /old_locus_tag="JFX43_026335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032330704.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQINELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            5273..5695
                     /locus_tag="JFX43_RS26340"
                     /old_locus_tag="JFX43_026340"
     CDS             5273..5695
                     /locus_tag="JFX43_RS26340"
                     /old_locus_tag="JFX43_026340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_032330703.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKSDTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     Typing          complement(1337..1418)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.59"
                     /coverage="93.0"
                     /evalue="3.21e-32"
     Typing          7082..7160
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.25"
                     /coverage="87.0"
                     /evalue="7.3e-29"
     Typing          1435..1992
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.405"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgacaaatc ctccatttca ggatttttta tgtagcgtct actacatgcg atccgttggt
       61 gagctgttta ttatgcatta gttatataat aactacatat tagttatact tttgttgtgt
      121 atcagttgca ggggcgtttt gagggtaaat tctggaggca cgtgaccgac tgtatacaaa
      181 atgctggcgc attttgatac gtcgacgcgc caaagtggac gcccctttga aaccccgcca
      241 gaattaaaag cggcctgtct ttttacagta ttcagctctg gctatacacc aacctatatt
      301 ttctcctttg gcataatgaa gcgcatcatt ccattttttc catgctccaa tgtaatcacc
      361 ttcacgctcc agtctgacag cttcgtttgc gggtgaaaaa tatttatttt ctaaataaat
      421 tgctttgtcc tgattcatac tcgtaattta tatttttatt tgaattaaaa aagagcggcc
      481 tgtcgggtta atacatattg gcatgactgt tgatatatca gcccaactgc acgctcgtaa
      541 tcgttttaac gtgattttcg caacatggca atccgggttc atctgtccgg taatcaataa
      601 aaaccgtgcg aaaaacggga caggatgtgt atttgtcgga ccgcgcacac gcaatcagac
      661 aaatgccgcc tagtcatggc tgcgccccga caccccgcca acgtctctgg ccagaactgg
      721 ccatcacgcc gggtcacttt gtcagatgtt aaaaaatcac gcggtaaacg ccgttattcg
      781 cgggtttgcg caagcacctc ccgtgcgtca ctttgtcaaa acgtcaaagt gacgcacatc
      841 aggaacagcc ggaacattcg tgtttatgac aacaactgca cacaacgcag acagaaaatc
      901 gcgcacatat tcagcaaaaa ataagcgtca cggggtgtta atgtcgcgca gcacacgcgc
      961 gactgggctt gtgtgataat tttcagtgac gtcaccgggt catcacacca ggtgacgcac
     1021 tgccggacgt aaacaccagt aaaggagacg ggagaacgtg aacaaacagc agcagaccgc
     1081 gctcaacatg gcgcgattta tcaaaagcca gagcctgacg ttgctcgaaa aactggatgc
     1141 actggatgca gacgaacagg ccgccatgtg cgaacgactg cacgaactgg cggaagaact
     1201 ccagaacagc atacagactc actttgaagc tcagtacggc atagaacaac cataaaggcg
     1261 ctggcgaaag tggtcgcggc ttgtccgtga gtgttttcgc cagcagacaa agcccgtcag
     1321 ggcacgtcag ggggctttag cgggcgtcgg ggcgcagcca tgacccagtc acgtagcggt
     1381 agcggagtgt atactggctt aatcatgtgg catcagtgcg gattgtatga aaagtgcacc
     1441 atgccgggtg tgaaatgccg catagatgcg taaggagaaa atgcagcttc cggtacactt
     1501 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggagtcac
     1561 ctcactcaaa gacggtgata ccgttatcca cagaatcagg ggataacgcc gaaagaaaca
     1621 tgtgcgcaaa aaacgagaac ccggaaaagg ccgcgccact ggcgtttttc cataggctcc
     1681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1741 gactataaag ataccaggcg tttccccccg gaagctccct cgtgcgctct cctgttccga
     1801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaaacgtg gcgctttctc
     1861 atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1981 ccaacccggt aagacacgac ttaacgccac tggcagcagc cattggtaac tggatagtgg
     2041 atttagatac gcagaactct tgaagttgaa gccttatagc ggctacactg gaaggacagc
     2101 atttggtatc tgtgctccac taaagccagt taccttcgga aaaagagttg gtagctcttg
     2161 atccggcaaa caaaccaccg ttggcagcgg tggttttttt gtttgcaagc agcagattac
     2221 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac cgcgatcccc
     2281 gtcagttcag aagacgaaga tggtgcaacg gttcctcctt gtacatcacg aatatagttt
     2341 gcttgacatc ctgacaagaa taatattgta attagaaaaa ttactatttt catacacctc
     2401 caacaaaaga aaaattatgt atctaaaata ctacctacat aatttacctg aatcacttat
     2461 accatggatt cttattttaa tatttaacga caatgataac actcctttgt tatttgtctt
     2521 tatatcatca atacatgtat tgctatatcc atactctaaa ttaaccatat ctagatatat
     2581 caaagaaaat acaaagttaa aaaaagagcc ctggtactta tgcaagttat ctgcattgtt
     2641 ttatttatta atggcaatcc cagtaggatt gccaagtttc atatattaca ctctaaagag
     2701 aaattaaatc cctaacaact cattaagttt gttcaactca tcatccccta tgtaagaaga
     2761 aacaatacct gtgacaattg caatccccca gaaaccaaga ggcacaccaa caataaaact
     2821 aaacattaat gccacaattt ttgccacacc tacatctacc gcacttttct ccagcgtgac
     2881 aaacaaaggt cgccagttac cagtttctat tgcattttta aaatcagtac caacatcata
     2941 tagaaaagat actcgactag tgattttaag cgactttgat atcttcgtta aattcttaga
     3001 taactcatca taattaacag actctaacgc attaaaaata gcatcccgat caaccttact
     3061 gaatttctta tccagtacat ttttatactt ttcaaatgct gccagagctt catcaacacc
     3121 ttgaattttc tttcctttag acttttccgc taaatcctga gcaatttttg cgtatttttc
     3181 tccatattgt tcggttatat attgataaaa tccaaccata gtatcaacag catcttttat
     3241 ctggctcttt tcaagagcat cctgagcttc ttttaatttc tgctctgcct ctttaacctc
     3301 agcggcctta ccatccctga aactaacagc attcttaagt tcattttcaa ctgcggagta
     3361 ctcagcctgc ttagcttcaa gctgacgctg tagttttttc tgaacgtcac gccatcccgg
     3421 aaatccggtg accttcacgt caacactctt ctgagcgttt tcaagctctc ctgcaactct
     3481 ggaaacagta gcttgcttac tctgtacatc actttcagcc ttcgccagtt cagctttcgc
     3541 ctcagctaaa cgcttctctg cttccaccac cgcctgtttc tcttcattaa gagtttcatg
     3601 ttcagcgacc tctgcatttt cacgagcctt ttcttcagct tccttctgtt tattcacatc
     3661 accaatttta ctttgcaatg tatttttata agaattaagt ttgctaatat ctgcatcaag
     3721 ctgatttgat tttttttgca actcatcaac atccctctca agatcagtga taccatgata
     3781 agaatgatgc ttgaatactt ttttcatctc ctcgattttc ttctgttttt cactaatctg
     3841 tgtagcaatt ttattcttct gcttttgttt ttcattaatt acattaccca ccaccttcga
     3901 actcttatcc atatcactga cctgagcatt cgttggtgca gtattaactg aagcagtgtt
     3961 attgttgtta tttcctgagc tttctgcaaa aagtgatggc atatcactaa ttaaagaatt
     4021 aagaactctg gagacccctc caaatggatt atcaaccaga gttgaattct cttctgtcat
     4081 aacaatacca ttcatcaccg gaaggccatc attattaatg acaacatcac cccacggagt
     4141 cagatatgac tcaccggttt tcatcacagt tgatgtagaa ccagatgaat ttgaatttcc
     4201 ctgaccacca ttattaccat gcccagagcc accaccccag tgaaccccac tattactatt
     4261 attatttcca ccttgctgat tcagattcgc ccctgttccc cccatagact caccagcagt
     4321 tgggccatat ccacttagtt ctttagccat aaattcctct ttgataatta aaacaataaa
     4381 ttaaaaacaa tatactgtat atataaccac tgattttatg tacagtgaaa acctactact
     4441 cagcattgtc cgtgtcaaga gcatggattt tcatttttgc aataaggatc acactatggg
     4501 gaggcaggca ttgagaacgt cgaaacagaa caccggagca aatcaggatg agatataaaa
     4561 ctgttggatc atgaaaaaac ggagaacgat gtgagcaaat caccccgcca taaactgaac
     4621 aaaacagaca aacgacttct cgacaccctt gttgctgccg gatatgagta tgacaaagcc
     4681 cgtgacctca tccagaaaca ggtttacacg ctgacactgg ctgatcagcg tcatgtggtc
     4741 agtgaaatca gtaatggtgt gaatcccacc caggcttact cagcggtata ccaggcaaga
     4801 cgcattcgcc tcgcccgtaa atatctgaac ggtaaaaagg ttatggaaga aaccggggaa
     4861 aatacgcccc catcagcgta aggatttctt ttgccgctcc agagactcca gttttttacg
     4921 caaatcctct cttttttggg catctctggt gccagccagc tctgctctca actcattgat
     4981 ctgaagttgt atcttcagtc tgttactgaa cattttctgt ctggcattaa catccgcaac
     5041 aatgccgttt tttgtcttct cggccttttg ttgaaaaaca ttgctgtccg catgactggc
     5101 aaccgaagca gaaagaacac taaaaagcag gactggcaca cattttttca cgggattatt
     5161 cctgactcat tgaccatcaa atcacattgg gagtaaaccg acgtatgata agagatactc
     5221 ttcggagata taactccctg agtatcaaga ttaaaaacgc aaggagatgt ttatgagatc
     5281 tgccgctgcc aggctgcttc tgatacctct gataacagca acaatagctc ttacaggatg
     5341 cacaccaaag accagcctgg aacgacatac ccggcattat gtttatgctt cagatgatgg
     5401 atttgatcct aacttctaca cccagaaatc agacaccata cgtatgatgc tccctttctt
     5461 tcagcagttc cgggatatgg ggatgaaaga caaagcagcc ggagtatcag cagaaacggc
     5521 acagcaacgt gtaaaagaat tccactcaga aaaatttttt cactcactcc ggagcacaac
     5581 aacctttgct ggcagaaaat acacaaacag cgatatgcct tcgccgaaaa aaatgaaact
     5641 aatggcagac accatttctg cggtttatct cgatggatac gagggcagac agtaagggat
     5701 ttaccataat cccttattgt acgcaccgct gaaacgcgct cagcgcgatt ctggcgcaaa
     5761 aaccgcaaaa agagatgttt tcccccgggt aaaccggaaa aatacgtcag gattgttttc
     5821 atggcactgc atgactatgc atgaaactga atggcgatcg gtttgggcgc gcctgatgcc
     5881 cataaggcgc attttcggac gttttcagcc ctgatgagaa gaaatcagac agtagttaca
     5941 gacgagtcgt gagcgattca ccccgggagt catcagcgag tcatccagta tttttcctcg
     6001 cgactctctg gcgactcgct ttttctgaac accagagaga cagtgtgttg agtcatcgat
     6061 aaatcaccga cgactcgttg ccgaatcatc cagtagtcgc cgacaactcg cttttgtata
     6121 aatctgaata agaaaatata tttttcaaac cataacaaca tgatttaaaa agcaaatcag
     6181 aaaaaagttg gttttgcgtg gggggtgggc atccggggca tgagaacaga ctcgcgtttt
     6241 tctggagaaa ctgcggggat ttttgattaa acaatactca tcgcagagcg gaattttatg
     6301 caacgccggc tgtgcggcac agggattttt aatccccagc atgtcagagg gtgcggatga
     6361 cggttcccgt ctggcgtttt catgggcgcc ggttgtcaaa aaataccagc gtgatcggtc
     6421 gaaggataag cgcagattaa tcagtgatga ttgagcgcga atctgacaac gagtatagcg
     6481 acacagcaac aaaaaagacg ccagaaacgc gctcagaggc tttgtaacgc gattttttac
     6541 atgaggcaat ggtttcgctt tgaggcgttc agccgtgttc agggcgcgat tctggtacat
     6601 acctgactgt aatcatccct acgaactact ctttgcacca gaaacgcgca gggtgtttta
     6661 gcgtgtctcc ggcgtgatgt cgagttgtgc ggtaatgagt gagcgaaaaa aatcccctgg
     6721 attacagggg gaacccatta tcagactttg aagtttattt gttatttgat ggagtgagct
     6781 cgaatcccag agatgacgtt caggtggcaa ggaaacgcgc tcagacgctt tctaacgcac
     6841 ttttttgcat ctggcaatgt ttcattatgt tttggtgggt tcaggctgtt agagacgttt
     6901 tgagcgcgtt tctggcgctt gctgctactg ccaaaccatc cccatggcca gttgatgcca
     6961 gaaacgcgca ggagttttgt agcgcgtctc cggtccctga tcaatgaatt cccggtcatt
     7021 aacatgcgtt tacccggaaa cgcgccctga tgttctatcg tatcgcggga acggtgtcga
     7081 ggctgtcggg gcaaagccct gaccaggtgg taatcgcata gccgtgcgtg cgcggttata
     7141 cgattacaca tcctgtcccg gaatcaggat tgtttttcgc gcacaactgc ggcaaattca
     7201 tcccgttgcg gttgcaacga tgggtatatc aaaactacga aaattattca ggcatttgct
     7261 accccccttg ccctggcctc caccagtcac gggggatttt tttgttgtct ctcatgacga
     7321 actacggcaa atttctgacg ttgcaaactg caacacataa aacacacaca aaactcacaa
     7381 ttgccatgtc ctcattgaca cccccttgcc atataggggg gattttttcg cctgtcctga
     7441 ttgtcttttg ccgatggtta cggcagattg gaaaagagcg catttgtggt gctctctcca
     7501 aaataatatt tctcattact caaactctga tggcttcccc ctgtaatcca gggggatttt
     7561 ttaatttatc gctatgcttc gtaattcatt cagaatacag acaatatggt taatcagttt
     7621 ttcatcattt ttgcggtgtt tgccctgtcc tgcatcagaa cgctaaaaaa acaggaatct
     7681 cttt
//
