LOCUS       NZ_CP075650             8121 bp    DNA     circular CON 27-JUN-2023
DEFINITION  Escherichia coli strain STEC17-190 plasmid pSTEC17_190-a, complete
            sequence.
ACCESSION   NZ_CP075650
VERSION     NZ_CP075650.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993369
            Assembly: GCF_016403975.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8121)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 8121)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: SEC17-190
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiniSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 05:54:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,972
            CDSs (total)                      :: 4,854
            Genes (coding)                    :: 4,698
            CDSs (with protein)               :: 4,698
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 59 of 156
            Pseudo Genes (incomplete)         :: 95 of 156
            Pseudo Genes (internal stop)      :: 42 of 156
            Pseudo Genes (multiple problems)  :: 38 of 156
            Pseudo Genes (short protein)      :: 3 of 156
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075650.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC17-190"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC17_190-a"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2017"
                     /collected_by="ILS/NENT"
     gene            1..927
                     /locus_tag="JFX56_RS24630"
                     /old_locus_tag="JFX56_24630"
     CDS             1..927
                     /locus_tag="JFX56_RS24630"
                     /old_locus_tag="JFX56_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271358.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNVSLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRGAVATS
                     ARTLKPWETLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1145..1420)
                     /locus_tag="JFX56_RS24635"
                     /old_locus_tag="JFX56_24635"
     CDS             complement(1145..1420)
                     /locus_tag="JFX56_RS24635"
                     /old_locus_tag="JFX56_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091751.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPKPPAKPQNH"
                     /name="hypothetical protein"
     gene            1599..3818
                     /gene="mobQ"
                     /locus_tag="JFX56_RS24640"
                     /old_locus_tag="JFX56_24640"
     CDS             1599..3818
                     /gene="mobQ"
                     /locus_tag="JFX56_RS24640"
                     /old_locus_tag="JFX56_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_024224537.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKEQDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSLGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            4051..5796
                     /gene="col"
                     /locus_tag="JFX56_RS24645"
                     /old_locus_tag="JFX56_24645"
     CDS             4051..5796
                     /gene="col"
                     /locus_tag="JFX56_RS24645"
                     /old_locus_tag="JFX56_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_009451403.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAVPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQ
                     FNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHID
                     IHRGQ"
                     /name="col"
     gene            5799..6056
                     /locus_tag="JFX56_RS24650"
                     /old_locus_tag="JFX56_24650"
     CDS             5799..6056
                     /locus_tag="JFX56_RS24650"
                     /old_locus_tag="JFX56_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            6118..6261
                     /locus_tag="JFX56_RS24655"
                     /old_locus_tag="JFX56_24655"
     CDS             6118..6261
                     /locus_tag="JFX56_RS24655"
                     /old_locus_tag="JFX56_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            6727..6867
                     /locus_tag="JFX56_RS24660"
                     /old_locus_tag="JFX56_24660"
     CDS             6727..6867
                     /locus_tag="JFX56_RS24660"
                     /old_locus_tag="JFX56_24660"
                     /inference="COORDINATES: protein motif:HMM:NF020569.2"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WP_231237649.1"
                     /translation="MYEWDSQHGELEGYRASDGQHLGSFDPKTGNQLKGPDPKRNIKKY
                     L"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            6877..7134
                     /locus_tag="JFX56_RS24665"
                     /old_locus_tag="JFX56_24665"
     CDS             6877..7134
                     /locus_tag="JFX56_RS24665"
                     /old_locus_tag="JFX56_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_000523347.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNIN
                     NGWFDVIAEWVPLLQPYFNHQIDISGNEYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     gene            7154..7420
                     /locus_tag="JFX56_RS24670"
                     /old_locus_tag="JFX56_24670"
     CDS             7154..7420
                     /locus_tag="JFX56_RS24670"
                     /old_locus_tag="JFX56_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000861936.1"
                     /translation="MKVELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFERL
                     TEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            7482..7625
                     /locus_tag="JFX56_RS24675"
                     /old_locus_tag="JFX56_24675"
     CDS             7482..7625
                     /locus_tag="JFX56_RS24675"
                     /old_locus_tag="JFX56_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..8121
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1425..1598)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(1599..1957)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..927
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.44"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1600..3815
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.917"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgaagaa aatttacccc accgtcccta tcacacggat
       61 gatctcgctt ttggtcttcg catctccggc aaagggcgtg cgcttcttgc gcggtacatc
      121 cagcagaacc agcctcatgc gcagttctgg ctggtttttg acgttgaccg cgagggggct
      181 gcgattgact ggagcgaccg gaacgccccc gcgccgaaca tcaccgtaaa aaatcccgtg
      241 aacggtcatg ctcacctgct ctatgcactc aacatcgccg tgagaaccgc gcctgatgcg
      301 tcggttaagg cactgaagta cgctgccgcg atagagcgtg cgctgtgtga gaaacttggt
      361 gcagatgtga actacagcgg actgatttgc aaaaatccgt tccacctgga atggctggtg
      421 atggagtggc gcgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagagc gtcgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggcctgt attctcacaa
      601 tggcttgatg ccgtgatcca gcgtgtcgaa atgtacaacg tatcgcttcc cgttccgctt
      661 tctccggcgg aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat tcaggctgca
      781 cgtggtcgca agggcgggaa aattggaggt gctaagtcta agcgtggtgc tgttgccaca
      841 tctgcgcgaa cgttgaaacc gtgggagact cttggaatta gccgcgcctg gtattaccaa
      901 ctgaaaaaac gaggtcttgt agagtagacc aaataaaacc tatatcagat aacagcgcct
      961 ttttggcgct tttttgagca gcttggtttg ttgctatttc cctcgttgaa tcccgcaatg
     1021 gcgcggcttt ccgcatgatt gaggttgtag cgctcgccgc agtctcatga ccgagcgtag
     1081 cgagcgaatg agcgaggaag cgcaaaggcg tccggtggtg catgtggctc ttacgcgccg
     1141 gggcttagtg gttctgcggt ttcgcaggtg gtttgggtag cttctccagc tcgttaatca
     1201 gcggttgtag tcggttcaca tccgcctgtc ttgtgacttc ctttcgcaga aactggagca
     1261 ggaacgcacg cagttgcgcc tcttccggcc tccgtaccct cgccagcatg gcagccccca
     1321 caatgacttt ttgcgccgtg tccaggctcc ggctcttcgc cttcaggcgc tgtaatctgg
     1381 cctcagcttc ggcaatcttc tgttcgagtg ttctgctcat tttgtgactc cgtgcgcggt
     1441 gaaaaatcgc attttatcgc gtcgctggta gttgcaaaac tgaactggca taatgcacgg
     1501 cacatcacga agtgcgcact tatacaatct ccacttcgtt tcgattgtgt gcggtctgcg
     1561 acgctaaaag aaaacggcaa aaaggcatta cggcagaaat ggcgatttat catctcagca
     1621 tgaaaatcat ttcgcgaaaa aacggctaca gtgctgttgc ttctgctgcc taccgttccg
     1681 gctctgtcat acccgatgac cgtaccggat taatccacga ttacacccgt aaacgcggcg
     1741 ttgatgatgc ggtcattctc acccctgcga atgcaccgtc ctggtgtggt gaccgttccg
     1801 ttctctggaa tgcggtcgag aaagccgaac agcgccggaa ctcccagctg gcaagggaga
     1861 ttgaactcgc cattccccgt gagatttccc gcgaggccgc acgggagacc gttctcgctt
     1921 ttgtccggga aaactttgtc agtcggggca tgattgccga tgtggcgttc catcacatgg
     1981 accggaccaa tccccatgcg cacatcatgc tgaccacgag agctgtcggg gaaacgggat
     2041 tcgcaggaaa ggtcagggac tggaacgacc gggcactcgc cgagacgtgg cgcgcatcat
     2101 gggctgacca tgcgaacaga gcgcttgcga acgccggcta ccaggaagag atagaccatc
     2161 gttcatacga gcgtcaggga ctggagaaag cgccgggcct tcacctcgga aaggctgcct
     2221 gtgcgatgga aaaacgggga atggaaacag aacgcggtga gcagaaccgc ctgattaaca
     2281 gccttaacct ggaaatacag gtttcccgca caaggctggc agtcatccag gtacaggaga
     2341 acgtgcggaa acgggaactc agcgatgccg cacgtcgtgc agcggaagcc cttaacctga
     2401 ccattcccgc tgcgaatgcc tcagcggata ccctgcggga attcattgcc acgttgccgc
     2461 aggaatgcgg caacgcgtgg gagatgaccc cggagttcct ggcgatgagc gggaaggtga
     2521 acgacatcga acgtgagggg aatgcgctgc tgaaagagca ggccattctc gaaaaggaga
     2581 tgaccggact caaaaaagca cgccctgtcg cgtcccttct gtcagagatt cccctgatga
     2641 catgggctga accggaatac cgcaaaagac aactccgttt ctggaaactc gggaaacaga
     2701 ttgaatctct tcgccgcacc tacagggccg tgaaagaaca ggacattccc gcccgtcgtc
     2761 aggcctttga aacgcagtgg aatacgtgga ttgcgccggg aatggcagag ctgaaagaaa
     2821 aactgtcagc acgggaagcg gagcggcgca gggaggaagc cgaagcggaa gcgcgccgga
     2881 aggaacagga gcatgaggcg cggctgaaac gtcatgataa ccaccgtctg agccgtgaaa
     2941 cggcattagt cggggttatt acggagctgg ggcgtgccag agagccggga acgggcagga
     3001 taacccgcta catgatgctg agtaacagag ccggagaatt cacggtatgg ggtgatgagc
     3061 tggcgcatta cccccagagt gttcatgacc cggtgaatgt ttacctgtcg ctaggcgggg
     3121 ctgtgatggt ctcggatata cgtgagggaa tgccagaatc tcatgagacg atggcgcggc
     3181 ctgagcgtgt gagaatgtat tccggtgcga cggtccggca tgtactggaa cagatgcgcc
     3241 aggggtggcc ctcttacggt tttccggcgc tgccgcatca ctggccggat aatttttatt
     3301 tcagcgacga ccgcaggccc gtagcctctc cgctgccgtc agcgcaccgg gtggatatca
     3361 ccgctgatgc tgtaccggaa caactcatgc ctgttgtatt ttcgagagag ggaaacagca
     3421 ggacgctgaa tctgttgtta tgcaaagggc cggaggaaat gcttgtcggg tttgttcgcc
     3481 gggaaggagg gttttgcccc gttcttgtgc ttccgtcgcc ggattacagt catctgatag
     3541 tcagcaccat cacggagaac ggggtatgcc tggcaggtta cggagaagct ataaaccatg
     3601 atgaggatac tccgtaccca ccggaaccgc acctgatgca gttccggctc aaaggccatc
     3661 atgacaggct tttggctgct gtccacaaac cggaagagat gccggattat ctcttccgtc
     3721 aactcggttt taatcagacc tggcatgagt ggaagcggga cgaacagcac aggcaacaac
     3781 aacgccgccc cgggcacttc cggggcatga gtatgtgatg tccggggctg caccccggac
     3841 cccgccaaca catcacgggc cacaaaattt tttgtggccc gctctgcgtt ttctaagtgt
     3901 tatccctcct gatttctaaa aaattttcca cctgagcttg acagaaaaaa cgatgacggg
     3961 tactttttga tctgtacata aaaccagtgg ttttatgtac agtattaatc atgtaattaa
     4021 ttgttttaac gcttaaaaga gggaattttt atgagcggtg gcgatggacg cggccataac
     4081 acgggcgcgc atagcacaag tggtaacatt aatggtggcc cgaccgggct tggtgtaggt
     4141 ggtggtgctt ctgatggctc cggatggagt tcggaaaata acccgtgggg tggtggttcc
     4201 ggtagcggca ttcactgggg tggtggttcc ggtcatggta atggcggggg gaatggtaat
     4261 tccggtggtg gctcgggaac aggcggtaat ctgtcagcag tagctgtgcc agtggcattt
     4321 ggttttccgg cactttccac tccaggagct ggcggtctgg cggtcagtat ttcagcggga
     4381 gcattatcgg cagctattgc tgatattatg gctgccctga aaggaccgtt taaatttggt
     4441 ctttgggggg tggctttata tggtgtattg ccatcacaaa tagcgaaaga tgaccccaat
     4501 atgatgtcaa agattgtgac gtcattaccc gcagatgata ttactgaatc acctgtcagt
     4561 tcattacctc tcgataaggc aacagtaaac gtaaatgttc gtgttgttga tgatgtaaaa
     4621 gacgaacgac agaatatttc ggttgtttca ggtgttccga tgagtgttcc ggtggttgat
     4681 gcaaaaccta ccgaacgtcc gggtgttttt acggcatcaa ttccaggtgc acctgttctg
     4741 aatatttcag ttaataacag tacgccagca gtacagacat taagcccagg tgttacaaat
     4801 aatactgata aggatgttcg cccggcagga tttactcagg gtggtaatac cagggatgca
     4861 gttattcgat tcccgaagga cagcggtcat aatgccgtat atgtttcagt gagtgatgtt
     4921 cttagccctg accaggtaaa acaacgtcag gatgaagaaa atcgccgtca gcaggaatgg
     4981 gatgctacgc atccggttga agcggctgag cgaaattatg aacgcgcgcg tgcagagctg
     5041 aatcaggcaa atgaagatgt tgccagaaat caggagcgac aggctaaagc tgttcaggtt
     5101 tataattcgc gtaaaagcga acttgatgca gcgaataaga ctcttgctga tgcaatagct
     5161 gaaataaaac aatttgatcg atttgcccat gacccaatgt ctggcggtca cagaatgtgg
     5221 caaatggccg ggcttaaagc ccagcgggcg cagacggatg taaataataa gcaggctgca
     5281 tttgatgctg ctgcaaaaga gaagtcagat gctgatgctg cattaagtgc cgcgcaggag
     5341 cgccgcaaac agaaggaaaa taaagaaaag gacgctaagg ataaattaga taaggagagt
     5401 aaacggaata agccagggaa ggcgacaggt aaaggtaaac cagttggtga taaatggctg
     5461 gatgatgcag gtaaagattc aggagcgcca attccagatc gcattgctga taagttgcgt
     5521 gataaagaat ttaaaaactt cgacgatttt cggagaaaat tctgggaaga agtgtcgaaa
     5581 gatcctgagt taagtaagca atttaatcca ggtaataaaa aacgcttgag ccaaggatta
     5641 gctccacggg ctaggaataa agatactgtc ggtgggcgtc gctcatttga gcttcatcac
     5701 gacaagccaa tcagtcagga tggtggtgtt tatgatatgg acaacctccg tatcactacg
     5761 ccgaaacgcc atattgatat tcacagaggt cagtaaaaat ggaactaaaa aacagcatta
     5821 gtgattacac tgaaactgaa ttcaaaaaaa ttattgaaga catcatcaat tgtgaaggtg
     5881 atgaaaaaaa acaggatgat aacctcgagc attttataag tgttactgag catcctagtg
     5941 gttctgatct gatttattac ccagaaggta ataatgatgg tagccctgaa gctgttatta
     6001 aagagattaa agaatggcga gctgctaacg gtaagtcagg atttaaacag ggctgaaata
     6061 tgaatgccag ttgtttaagg atgaatgact ggcattcttt cacaacaagg agtcgttatg
     6121 aaaaaaataa cagggattat tttattgctt cttgcagtca ttattctggc tgcatgtcag
     6181 gcaaactata tccgggatgt tcagggcggg accgtatctc cgtcatcaac agctgaagtg
     6241 accggattag caacgcagta acccgaaatc ctctttgaca aaaacaaagc gtgtcaggct
     6301 gattctgatg cgcttttttt gaaatgtcac aaaagttcca tgtgggagat gggatctaaa
     6361 atccctgtgc agaaccttcc atccggtggg agaaaacttg tcattttgac ctgttcgcca
     6421 ttcggggggg ggcgcttgga aaacggaaat tttcccacgc taagagaatt gagttgaacg
     6481 agtggttaca tctaggccag ctaactcact gaacgtaaag gctgtacgaa atgttccgac
     6541 cgtccgataa ccatttggct tacttccgta catttccttg cgtagatgta cagaattgta
     6601 cgatttcgga caccaaaacc gctgttgtcc cgccattctg gcgattcctg aaagttattt
     6661 gactaaaaaa tagtcttaac gtggtttatt ttccgaattc caagcggacc ccataaaggc
     6721 cgtaagattt atgaatggga ctcccagcac ggtgaacttg aagggtatcg tgccagtgat
     6781 ggtcagcatc ttggctcatt cgatcctaag actggtaatc agttgaaagg acctgatcca
     6841 aaacgaaata tcaaaaagta tctttgagag gatgttatgg ggcttaaatt acatattaat
     6901 tggtttgata agacgaccga ggaatttaaa ggtggtgagt attcaaaaga tttcggtgat
     6961 gatggctcgg tcattgaacg tcttggaatg cctttaaaag ataatatcaa taatggttgg
     7021 tttgatgtta tagctgaatg ggtacctttg ctacaaccat actttaatca tcaaattgat
     7081 atttccggta atgagtattt tgtttcattt gattatcgtg atggtgattg gtgatcaaat
     7141 attctcagga gagatgaagg tggagctaaa gaaaagtatt agtgattata ccgaggctga
     7201 atttctggag cttgtaaaaa aaatatgtag agctgaaggt gctactgaag aggatgacaa
     7261 taaattagtg agagagtttg agcgattaac tgagcaccca gatggttcag atctgattta
     7321 ttatcctcgc gatgacaggg aagatagtcc tgaagggatt gtcaaggaaa ttaaagaatg
     7381 gcgagctgct aacggtaagt caggatttaa acagggctga aatatgaatg ccggttgttt
     7441 atggatgaat ggctggcatt ctttcacaac aaggagtcgt tatgaaaaaa ataacaggga
     7501 ttattttatt gcttcttgca gtcattattc tgtctgcatg tcaggcaaac tatatccggg
     7561 atgttcaggg cgggaccgta tctccgtcat caacagctga agtgaccgga ttagcaacgc
     7621 agtaacccga aatcctcttt gacaaaaaca aagcgtgtca ggctgattct gatgcgcttt
     7681 tttttttgaa atgtcacaaa aattccatgt gggagatggg atctaaaatc ctcgtgcaga
     7741 actttccatc cagggggaga aaacttgtcg ttttgagccg ttcggtgttc agaacgcacg
     7801 aaaccgatcg cgcatcgctt tcgtgaatag ttatgcaggc ccctgaaaac gattctgacg
     7861 cgttttttcg gttttgcctg gtgttttcct gtctttttgc gttttttgcg tcagagcgcg
     7921 tctgagggcg ttttaagggg tgcgtacaac gggagttatg gtaaatggat cgggttttcg
     7981 ggaaggttcg acaggatttg ccgttgggtg tagtgtaagc gactgaaaaa caaacgcccc
     8041 tgaaattcgt gtctctccgc caagattgtc acgagaatac aggggcgagg tcggtcgata
     8101 acctggaagg aatcgtaaca c
//
