LOCUS       NZ_CP075645             6754 bp    DNA     circular CON 27-JUN-2023
DEFINITION  Escherichia coli strain S19-141 plasmid pS19_141-e, complete
            sequence.
ACCESSION   NZ_CP075645
VERSION     NZ_CP075645.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993351
            Assembly: GCF_016404325.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 6754)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: S19-141_NP
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:11:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,151
            CDSs (total)                      :: 5,035
            Genes (coding)                    :: 4,825
            CDSs (with protein)               :: 4,825
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 90 of 210
            Pseudo Genes (incomplete)         :: 109 of 210
            Pseudo Genes (internal stop)      :: 63 of 210
            Pseudo Genes (multiple problems)  :: 49 of 210
            Pseudo Genes (short protein)      :: 5 of 210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075645.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S19-141"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pS19_141-e"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2019"
                     /collected_by="ILS/NENT"
     gene            complement(join(6701..6754,1..90))
                     /locus_tag="JFX59_RS25555"
                     /old_locus_tag="JFX59_025555"
     CDS             complement(join(6701..6754,1..90))
                     /locus_tag="JFX59_RS25555"
                     /old_locus_tag="JFX59_025555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(152..409)
                     /locus_tag="JFX59_RS25560"
                     /old_locus_tag="JFX59_025560"
     CDS             complement(152..409)
                     /locus_tag="JFX59_RS25560"
                     /old_locus_tag="JFX59_025560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(412..2157)
                     /gene="col"
                     /locus_tag="JFX59_RS25565"
                     /old_locus_tag="JFX59_025565"
     CDS             complement(412..2157)
                     /gene="col"
                     /locus_tag="JFX59_RS25565"
                     /old_locus_tag="JFX59_025565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_052986704.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADMMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQ
                     FNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHID
                     IHRGQ"
                     /name="col"
     gene            complement(2390..4609)
                     /gene="mobQ"
                     /locus_tag="JFX59_RS25570"
                     /old_locus_tag="JFX59_025570"
     CDS             complement(2390..4609)
                     /gene="mobQ"
                     /locus_tag="JFX59_RS25570"
                     /old_locus_tag="JFX59_025570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_052986705.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMVRPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            4788..5063
                     /locus_tag="JFX59_RS25575"
                     /old_locus_tag="JFX59_025575"
     CDS             4788..5063
                     /locus_tag="JFX59_RS25575"
                     /old_locus_tag="JFX59_025575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091752.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            complement(5279..6172)
                     /locus_tag="JFX59_RS25580"
                     /old_locus_tag="JFX59_025580"
     CDS             complement(5279..6172)
                     /locus_tag="JFX59_RS25580"
                     /old_locus_tag="JFX59_025580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277133.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     BGC             1..6754
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4252..4610
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4611..4784
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(5280..6172)
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.051"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2394..4609)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.865"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccctgaaca tcccggatat agtttgcctg acatgcagcc agaataatga ctgcaagaag
       61 caataaaata atccctgtta tttttttcat aacgactcct tgttgtgaaa gaatgccagt
      121 cattcatcct taaacaactg gcattcatat ttcagccctg tttaaatcct gacttaccgt
      181 tagcagctcg ccattcttta atctctttaa taacagcttc agggctacca tcattattac
      241 cttctgggta ataaatcaga tcagaaccac taggatgctc agtaacactt ataaaatgct
      301 cgaggttatc atcctgtttt ttttcatcac cttcacaatt gatgatgtct tcaataattt
      361 ttttgaattc agtttcagtg taatcactaa tgctgttttt cagttccatt tttactgacc
      421 tctgtgaata tcaatatggc gtttcggcgt agtgatacgg aggttgtcca tatcataaac
      481 accaccatcc tgactgattg gcttgtcgtg atgaagctca aatgagcgac gcccaccgac
      541 agtatcttta ttcctagccc gtggagctaa tccttggctt aagcgttttt tattacctgg
      601 attaaattgc ttacttaact caggatcttt cgacacttct tcccagaatt ttctccgaaa
      661 atcgtcgaag tttttaaatt ctttatcacg caacttatca gcaatgcgat ctggaattgg
      721 cgctcctgaa tctttacctg catcatccag ccatttatca ccaactggtt tacctttacc
      781 tgtcgccttc cctggcttat tccgtttact ctccttatct aatttatcct tagcgtcctt
      841 ttctttattt tccttctgtt tgcggcgctc ctgcgcggca cttaatgcag catcagcatc
      901 tgacttctct tttgcagcag catcaaatgc agcctgctta ttatttacat ccgtctgcgc
      961 ccgctgggct ttaagcccgg ccatttgcca cattctgtga ccgccagaca ttgggtcatg
     1021 ggcaaatcga tcaaattgtt ttatttcagc tattgcatca gcaagagtct tattcgctgc
     1081 atcaagttcg cttttacgcg aattataaac ctgaacagct ttagcctgtc gctcctgatt
     1141 tctggcaaca tcttcatttg cctgattcag ctctgcacgc gcgcgttcat aatttcgctc
     1201 agccgcttca accggatgcg tagcatccca ttcctgctga cggcgatttt cttcatcttg
     1261 acgttgtttt acctggtcag ggctaagaac atcactcact gaaacatata cggcattatg
     1321 accgctgtcc ttcgggaatc gaataactgc atccctggta ttacccccct gagtaaatcc
     1381 tgccgggcga acatccttat cagtattatt tgtaacacct gggcttaatg tctgtactgc
     1441 tggcgtactg ttattaactg aaatattcag aacaggtgca cctggaattg atgccgtaaa
     1501 aacacctgga cgttcggtag gttttgcatc aaccaccgga acactcatcg gaacacctga
     1561 aacaaccgaa atattctgtc gttcgtcttt tacatcatca acaacacgaa catttacgtt
     1621 tactgttgcc ttatcgagag gtaatgaact gacaggtgat tcagtaatat catctgcggg
     1681 taatgacgtc acaatctttg acatcatatt ggggtcatct ttcgctattt gtgatggcaa
     1741 tacaccatat aaagccaccc cccaaagacc aaatttaaac ggtcctttca gggcagccat
     1801 catatcagca atagctgccg ataatgctcc cgctgaaata ctgaccgcca gaccgccagc
     1861 tcctggagtg gaaagtgccg gaaaaccaaa tgccactggc gcagctactg ctgacagatt
     1921 accgcctgtt cccgagccac caccggaatt accattcccc ccgccattac catgaccgga
     1981 accaccaccc cagtgaatgc cgctaccgga accaccaccc cacgggttat tttccgaact
     2041 ccacccggaa ccatcagaag caccaccacc tacaccaagc ccggtcgggc caccattaat
     2101 gttaccactt gtgctatgcg cgcccgtgtt atggccgcgt ccatcgccac cgctcataaa
     2161 aactccctct tttaagcgtt aaaacaatta attacatgat taatactgta cataaaacca
     2221 ctggttttat gtacagatca aaaagtaccc gtcatcgttt tttctgtcaa gttcaggtgg
     2281 aaaatttttt agaaatcagg agggataaca cttagaaaac gcagagcggg ccacaaaaaa
     2341 ttttgtggcc cgtgatgtgt tggcggggtc cggggtgcag ccccggatat cacatactca
     2401 tgccccggaa gtgcccgggg cggcgttgtt gttgcctgtg ctgttcgtcc cgcttccact
     2461 catgccaggt ctgattaaaa ccgagttgac ggaagagata atccggcatc tcttccggtt
     2521 tgtggacagc agccaaaagc ctgtcatgat ggcctttgag ccggaactgc atcaggtgcg
     2581 gttccggtgg gtacggagta tcctcatcat ggtttatagc ttctccgtaa cctgccaggc
     2641 ataccccgtt ctccgtgatg gtgctgacta tcagatgact gtaatccggc gacggaagca
     2701 caagaacggg gcaaaaccct ccttcccgtc gaacaaaccc gacaagcatt tcctccggcc
     2761 ctttgcataa caacagattc agcgtcctgc tgtttccctc tctcgaaaat acaacaggca
     2821 tgagttgttc cggtacagca tcagcggtga tatccacccg gtgcgctgac ggcagcggag
     2881 aggctacggg cctgcggtcg tcgctgaaat aaaaattatc cggccagtga tgcggcagcg
     2941 ccggaaaacc gtaagagggc cacccctggc gcatctgttc cagtacatgc cggaccgtcg
     3001 caccggaata cattctcaca cgctcaggcc gcaccatcgt ctcatgagat tctggcattc
     3061 cctcgcgtat atccgagacc atcacagccc cgcctggcga caggtaaaca ttcaccgggt
     3121 catgaacact ctgggggtaa tgcgccagct catcacccca taccgtgaat tctccggctc
     3181 tgttactcag catcatgtag cgggttatcc tgcccgttcc cggctctctg gcacgcccca
     3241 gctccgtaat aaccccgact aatgccgttt cacggctcag acggtggtta tcatgacgtt
     3301 tcagccgcgc ctcatgctcc tgttccttcc ggcgcgcttc cgcttcggct tcctccctgc
     3361 gccgctccgc ttcccgtgct gacagttttt ctttcagctc tgccattccc ggcgcaatcc
     3421 acgtattcca ctgcgtttca aaggcctgac gacgggcggg aatgtcccgt tctttcacgg
     3481 ccctgtaggt gcggcgaaga gattcaatct gtttcccgag tttccagaaa cggagttgtc
     3541 ttttgcggta ttccggttca gcccatgtca tcaggggaat ctctgacaga agggacgcga
     3601 cagggcgtgc ttttttgagt ccggtcatct ccttttcgag aatggcctgc tctttcagca
     3661 gcgcattccc ctcacgttcg atgtcgttca ccttcccgct catcgccagg aactccgggg
     3721 tcatctccca cgcgttcccg cattcctgcg gcagcgtggc aatgaattcc cgcagggtat
     3781 ccgctgaggc attcgcagcg ggaatggtca ggttaagggc ttccgctgca cgacgtgcag
     3841 catcgctgag ttcccgctta cgctgcgctt cctgaaccgt cctgagagca agctgcgtgc
     3901 gggaaacctg tatttccagg ttaaggctgt taatcagacg gttctgctca ccgcgttctg
     3961 tttccattcc ccgtttttcc atcgcacagg cagcctttcc gaggtgaagg cccggcgctt
     4021 tctccagtcc ctgacgctcg tatgaacgat ggtctatctc ttcctggtag ccggcgttcg
     4081 caagcgctct gttcgcatgg tcagcccatg atgcgcgcca cgtctcggcg agtgcccggt
     4141 cgttccagtc cctgaccttt cctgcgaatc ccgtttcccc gacagctctc gtggtcagca
     4201 tgatgtgcgc atggggattg gtccggtcca tgtgatggaa cgccacatcg gcaatcatgc
     4261 cccgactgac aaagttttcc cggacaaaag cgagaacggt ctcccgtgcg gcctcgcggg
     4321 aaatctcacg gggaatggca agttcaatct cccttgccag ctgggagttc cggcgctgtt
     4381 cggctttctc gaccgcattc cagagaacgg aacggtcacc acaccaggac ggtgcattcg
     4441 caggggtgag aatgaccgca tcatcaacgc cgcgtttacg ggtgtaatcg tggattaatc
     4501 cggtacggtc atcgggtatg acagagccgg aacggtaggc agcagaagca acagcactgt
     4561 agccgttttt tcgcgaaatg attttcatgc tgagatgata aatcgccatt tctgccgtaa
     4621 tgcctttttg ccgttttctt ttagcgtcgc agaccgcaca caatcgaaac gaagtggaga
     4681 ttgtataagt gcgcatttcg tgatgtgccg tgcattatgc cagttcagtt ttgcaactac
     4741 cagtgacgcg ctaaaatgcg atttttcacc gcgcacggag tcacgaaatg agcagaacac
     4801 tcgaacagaa gattgccgaa gctgaggcca gattacagcg cctgaaggcg aagagccgga
     4861 gcctggacac ggcgcaaaaa gtcattgtgg gggctgccat gctggcgaga gtacggaggc
     4921 ctgaagaagc gcaactgcgt gcgttcctgc tccagtttct gcgaaaggaa gtcacaagac
     4981 aggcggatgt gaaccgacta caaccgctga ttaacgagct ggagaagcta cccagaccac
     5041 cggcgaaacc gcagaaccac taagccccgg cgcgtaagtg cccatgcacc accgggcgcc
     5101 tttgcgcttc ctcgctcatt cgctcgctac gctcggtcat gagactgcgg cgagcgctac
     5161 cacctcaatc atgcggaaag ccacgccatt acggggcaga acggaggaaa tcgcaagaaa
     5221 gatcactgct caaaaagacg ccaaaagggc gctgttatct gataaggctt atctggtctc
     5281 attttgcgag atctggatca cattttaatt ttcggtagta cgtcgctcga ctgatcccta
     5341 atgcctccca cggcttcagc gaacgtgctg atgtaggaac tgctgcgcgc ttagatttag
     5401 ttccaccctt gcgacctcga gcggcctgta cagcggaaaa tccctctggt gagaatttcc
     5461 tgtgtgtata tttcgcaatg ctcttgccaa tagcccgaca ttcagccgga gaaagcggaa
     5521 cgggaagcga tgcgttgtac atttcgacac gctggatcac ggcatcaagc cattgtgaga
     5581 atacaggcca gccctgacga atcgctctgt aggcccattt acggaccttt tcgaacagat
     5641 ggtagtttcg ccccagcccg taatttttat cgacgctacg gcgcgctgag gcgctcaaat
     5701 cgagataatc agccagttcg tcgagagtgt agggttcctc gcgccattcc acttcctgcc
     5761 attcagggtg gcacggattt ttgcagatca ggccgctgta attcacatcc gcgcccagtt
     5821 tttcacacaa cgcacgctca atagcggcag cgtatctgag cgccgaagcc gatgcatccg
     5881 gcgcagttct cacaggaagg gcgagcgcgt agagcagatg cgcgtgcccg ttacggggat
     5941 tttttacggt gatgttaggg gccggacaat tccggtcgct ccagtcgatt gatgcccctg
     6001 tccggtccac gtcaaaggga agccagtaca gtgcatgtgg ctggttctgc tggatgaaac
     6061 gggcaagaat ggcaatgttt ttcggcagaa tgcgaacgcc gtacgcgaaa tcgttcgtac
     6121 agtacggttt gtgcggtaat ttttccctga agcgttgaag tacggcgctc atgtgatacg
     6181 atttctctcg gtacacagtt taactaaccg cgctttccca tcgggaaacg cggaacccgg
     6241 cgccctgtaa tttcgtgatc aatcttggcg gtgagagcac gatttacggg gcgtttgttt
     6301 ttcagtcgct tacactacac ccaacggcaa atcctgtcga tccttcccga aaaaccgatc
     6361 catttaccat aactcccgtt gtacgtaccc cttaaaacac cctcaatcgc tttctgacgc
     6421 aaaaaaaaca aaaagacaag gaaacaccag ggtaaacata aaaaatgcgt cagaatcgtt
     6481 tttagcgcgt tgcataacta tgcacgaaag cgatgcacga tcggtttcgt gcgttctgaa
     6541 caccgaacgg ctcaaaacga caagttttct ccccctggat ggaaagttct gcacggggat
     6601 tttagatccc atctcccaca tggaattttt gtgacacttc aaaaaaaagc gcgtcagaat
     6661 cagcctgaca cgctttgttt ttgtcaaaga ggatttcggg ttactgcgtt gctaatccgg
     6721 tcacttcagc tgttgatgac ggagatacgg tccc
//
