LOCUS       NZ_CP075644             6995 bp    DNA     circular CON 27-JUN-2023
DEFINITION  Escherichia coli strain S19-141 plasmid pS19_141-d, complete
            sequence.
ACCESSION   NZ_CP075644
VERSION     NZ_CP075644.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993351
            Assembly: GCF_016404325.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 6995)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: S19-141_NP
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:11:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,151
            CDSs (total)                      :: 5,035
            Genes (coding)                    :: 4,825
            CDSs (with protein)               :: 4,825
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 90 of 210
            Pseudo Genes (incomplete)         :: 109 of 210
            Pseudo Genes (internal stop)      :: 63 of 210
            Pseudo Genes (multiple problems)  :: 49 of 210
            Pseudo Genes (short protein)      :: 5 of 210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075644.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S19-141"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pS19_141-d"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2019"
                     /collected_by="ILS/NENT"
     gene            complement(join(6801..6995,1..366))
                     /locus_tag="JFX59_RS25500"
                     /old_locus_tag="JFX59_025500"
     CDS             complement(join(6801..6995,1..366))
                     /locus_tag="JFX59_RS25500"
                     /old_locus_tag="JFX59_025500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_074167501.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<395..835)
                     /locus_tag="JFX59_RS25955"
                     /pseudo=""
     CDS             complement(<395..835)
                     /locus_tag="JFX59_RS25955"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1044..1367)
                     /locus_tag="JFX59_RS25510"
                     /old_locus_tag="JFX59_025510"
     CDS             complement(1044..1367)
                     /locus_tag="JFX59_RS25510"
                     /old_locus_tag="JFX59_025510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1435..1626
                     /locus_tag="JFX59_RS25515"
                     /old_locus_tag="JFX59_025515"
     CDS             1435..1626
                     /locus_tag="JFX59_RS25515"
                     /old_locus_tag="JFX59_025515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_198779963.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELNADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2291..2428
                     /locus_tag="JFX59_RS25520"
                     /old_locus_tag="JFX59_025520"
     CDS             2291..2428
                     /locus_tag="JFX59_RS25520"
                     /old_locus_tag="JFX59_025520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_197732592.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWRFAGINL
                     "
                     /name="replication initiation protein"
     gene            complement(2637..2783)
                     /locus_tag="JFX59_RS25525"
                     /old_locus_tag="JFX59_025525"
     CDS             complement(2637..2783)
                     /locus_tag="JFX59_RS25525"
                     /old_locus_tag="JFX59_025525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_040077440.1"
                     /translation="MRKIKWIFGLVIIGVIVIAGCQANYIRDVQGGTVAPSSSSELTGI
                     ATQ"
                     /name="colicin release lysis protein"
     gene            complement(2873..3145)
                     /locus_tag="JFX59_RS25530"
                     /old_locus_tag="JFX59_025530"
     CDS             complement(2873..3145)
                     /locus_tag="JFX59_RS25530"
                     /old_locus_tag="JFX59_025530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042968276.1"
                     /translation="MIDFERMSKEESIADVIAFLVRKEGGFGYPQMDRFFSRHNFSVIE
                     SGEFMRVFEQLCQAGVVVLGDKMLVKKGPNWKEPQFVSDKKYGIN"
                     /name="hypothetical protein"
     gene            complement(3142..5385)
                     /locus_tag="JFX59_RS25960"
     CDS             complement(3142..5385)
                     /locus_tag="JFX59_RS25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016928078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="WP_225376819.1"
                     /translation="MTNSLGYSGTPISHYVDGVPYDFNGSPIIVITNGPEKPVINTGVL
                     DKLPPVDKPIFLDPSIFNKEVTSVSDLKKIIVALESAIKHLTSLLPSDINKFVSSVKTL
                     LGEVRGNSSFTVLVNELDTALSRLEKSIEDVNSGIVVVDERRHNVETMLNQFTDKFKYK
                     EFLDKSKQLGDFSYFEGAFIIGLASRPDTRWSITSWKEMVVLPNKELFDAQNELITKYN
                     HLLHEYEIVSKKVDILSVEKSRLESGTSLTAPEKPDLIEPSNNGKPDTLPGKAELLIPV
                     LPPPDIHIPEIPTIQALDLENIKIIELELADFKPADIDSVEIKPIEIPPVDINPWPSSA
                     LGSEVTDPVYTPEMVKKAESSMLAAGALVMGVAPQGMQLSVAGRGAWIPVAEIAGNIAT
                     RILSAVKSLMAVNPITLSASVTVAGLWPKEAGKNSDKVPGRDVEALFAVNARLLAEDIQ
                     MSEFSRMEHIPVRGAIVMDNGRPVIRFFNTGATGRFPFVPVLKAERDELTGLDKIIVPG
                     INGGPSRTILINPVPVPSVPTDTGNTSPVPVTPVHTGTEVKPVDNIGVTTSPVPEDIVL
                     NDFIYWQPREHGTVYEPIYVMLSGIYGETNAKGKYSHRPYNTDKAGGPIQNLDWKNTEI
                     DRVGVDKVKLHTGRFEESDANKVMIDRLEKILKGELQVTDTDKRFYTHEIRELERYRNL
                     GVKDGTIPDNAGEVWNNTHTATLEDYKLSNDETLLYTPEALKAAEEQELRMLK"
                     /name="S-type pyocin domain-containing protein"
     gene            complement(<5696..5866)
                     /locus_tag="JFX59_RS25540"
                     /old_locus_tag="JFX59_025540"
                     /pseudo=""
     CDS             complement(<5696..5866)
                     /locus_tag="JFX59_RS25540"
                     /old_locus_tag="JFX59_025540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485271.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(6008..6409)
                     /locus_tag="JFX59_RS25545"
                     /old_locus_tag="JFX59_025545"
     CDS             complement(6008..6409)
                     /locus_tag="JFX59_RS25545"
                     /old_locus_tag="JFX59_025545"
                     /inference="COORDINATES: protein motif:HMM:NF016255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_052074460.1"
                     /translation="MSEQEEREQKRREYRALLEGFWPDAFNFSRPRPLKVGILAELIAD
                     AEQRGLPFDSELLTSAIKFYTRRYVYQRALTTMKERTGLDGQIAGEVTPKQREYSLKLI
                     RRFAAKDKAEKRARQEEKAAKQSGINPGA"
                     /name="ProQ/FINO family protein"
     gene            complement(6561..6794)
                     /locus_tag="JFX59_RS25550"
                     /old_locus_tag="JFX59_025550"
     CDS             complement(6561..6794)
                     /locus_tag="JFX59_RS25550"
                     /old_locus_tag="JFX59_025550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1712..1799)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          1810..2363
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.761"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..1054)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.43"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1427..1629)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="4.54e-12"
     Typing          complement(6458..6993)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.765"
                     /coverage="99.0"
                     /evalue="6.15e-79"
ORIGIN
        1 ctgccactga atcagccccc atgacgccag aaaaatcccg cccgtgacgc aaagcgccgt
       61 cagcgacggg tacagtatcc gccctttgat cagcttccag agcagctctt cctgccgccg
      121 ggccagttcg ttctctgtgg cgctgaactg cgcgttcacg gcactgttca gcgtctccag
      181 ttgttctttc accgctgctg tgtgtgcgct gatagcgtct ctgattttct gcccgtttaa
      241 gttcagttcc ctgtctacag acgcttcgag cttcctgaac tcgctgttca gcatgttctc
      301 tgtagagacg gcacgctctt tcagtttctt ctcgaagtct gtccccattt gtaaaagatt
      361 gctcatacag cgcccctttc agcctgagat tacgcccgcc ctccgggtcg gcgatactga
      421 tactgctcct ggttgtcctc accacctcaa aacctgccgc tgtaagcgcc tcagtgacat
      481 cctgacgcgt ttttagcgct ccggcatggt aaagagcctc cagtcccctc gtaatcgctt
      541 ctgcggcctc ctgtttcgct ttcggcagat tattcggggt gacaagtgtc cgcctgttct
      601 ccggtgcgtt cgggtcgtgc agcccgtaat ggtgattcac cagtgtctgc caggcattga
      661 ttcgcggacg gtccgcccgg tcgtaatagg gctggagccg ttttccgctc gccagctcca
      721 tattcgggat gacaaaattc agctcaagac gccccttgtc ctggtgctcc acccacagga
      781 tgctgtactg atttttttca agaccgggca tcagtacccg ctcaaagctc tccatcaccc
      841 tttcacgctc tcccggtggc agggtctgct ctgcaaaaga cagaaccccc gaggtgtatt
      901 ttttcgcaaa cggcgtcgca tcgatgagtt cccgtacctc ttcgggagca ccccgcagaa
      961 ctctcgcccc ttcccggtta cgctcccggc ccagcaggta atcaaccgga ccactgccac
     1021 cgccttttcc cctggcatga aacttaacta tcatcccgtt ctccctgttt acggacctca
     1081 tccctcagct cactcagttc acgtccgatg gccatcagtg cagccaccac atgaacccgg
     1141 tcatgccccg accactgtcc gctgtttatc ttccgggcta tctgattcag gttattgccg
     1201 accgaagcga actggcgcaa cagcggcggt gccagtgtcg gaagacctga cgttttcgat
     1261 ggcggtgccc ccaggcagac cttacgcatc catgacgcaa gttgttttcc ctcacaacgt
     1321 gccagcagcc gcgcatgttc ctcatccgtg acccgtatcg tgagcatcct ttcgcgtttc
     1381 accggtatca ttaaaaacct ccgacagact ccccacacat ggagaaacag aactgtgact
     1441 aaacaggaaa aaaccgccct taacatggcc cgcttcatca aaagccagac gctgaccctg
     1501 ctggaaaaac tgaatgaact gaacgccgac gaccaggcgg acatctgcga agcgcttcac
     1561 gaccacgctg acgagcttta ccgcagctgc ctcgcacgct tcggggatga cggtgaaaac
     1621 ctctgacaca tgcagctccc ggagacggtc acagctggcc tgtgagcgga tgccgggagc
     1681 agacaagtcc gtcagggcgc gtcagcaggt tttagcgggt gtcggggcgc agccatgacc
     1741 cagtcacgta gcgatagcgg agtgtatact ggcttaatta tgcggcaaca gtgcggattg
     1801 tatgaaaagt gcgccatgcc aggtgtgaaa tgccgcacag atgcgtaagg agaaaatgca
     1861 cgtccagacg cttttccgct tcctcgctca ctgactcgct gcgctcggtc gttcgactgc
     1921 ggcgagcgga gtctgcttgc tcaaaaacgg cgatacggtt atccacagaa tcaggggata
     1981 aggccggaaa gaacatgtga gcaaaaaggc aaagaaaaag tcgcgccgct ggcgtttttc
     2041 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2101 aacccgacag gactataaag ataccaggcg tttccccccc ggaagctccc tcgtgcgctc
     2161 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2221 ggcgctttct catagctcac gctgttggta tctcagttcg gtgtaggtcg ttcgctccaa
     2281 gctgggctgt atgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2341 tcgtcttgag tccaacccgg taagacacga cttaacgcca ctggcagcag ccattggtaa
     2401 ctggaggttt gcgggaatta atctctaaaa gaagtcttga agtaatgccc tgactgcggc
     2461 tacactagaa gaacaagttt tggtgactgt tcctgctaag aatccagtta ccgtccggtt
     2521 tcgaagagtt gctagctcaa cgaacccgaa ctaacccgcc atccggcggg ttttttcgtt
     2581 tacaggcaga gattacgcgt agaaaaaaag gatctcaaga gatcctttga tcttttctac
     2641 tgagtagcga tccccgtcag ttcagaagag gatgatggcg caacagtccc tccttgtaca
     2701 tcacgaatat agtttgcctg acagccggca attacaatca caccaataat aaccaatcca
     2761 aatatccatt ttatttttct cataatcaat ccttatcatg agttaaatat cagtctccca
     2821 tccatgaaag actgatattt agaacaacag ctaatccatt aactgtttct acttaattaa
     2881 tgccatattt tttatcagaa acaaattgag gttccttcca attaggtcct ttcttaacaa
     2941 gcattttatc acccaatacg acaacacctg cctgacataa ttgctcaaaa actctcataa
     3001 attcgccact ttcaattact gagaagttat gacggctaaa aaaacgatcc atctgagggt
     3061 atccaaatcc gccttccttt ctcactagaa atgcaataac atcagcaata ctctcttctt
     3121 ttgacatccg ttcaaaatca atcattttag catcctcaat tcttgttctt ccgcggcttt
     3181 taaagcttct ggtgtatata aaagagtctc gtcgttactt aatttataat cttccaacgt
     3241 agcggtatga gtgttattcc aaacttcccc cgcattgtca ggaatggtgc catctttaac
     3301 accaagattg cgataccttt ccagctctct aatctcatgg gtataaaagc gtttatccgt
     3361 atcagtaact tgcaattcgc ctttcaggat tttctccaag cggtcgatca ttactttgtt
     3421 agcatccgac tcttcaaaac gcccggtatg tagtttcacc ttatcaacac ctacgcgatc
     3481 aatctcggta tttttccagt ccagattttg aataggaccg cctgctttat ccgtattata
     3541 tggacgatga ctgtacttcc ctttagcatt tgtttctcca taaataccac tcagcatcac
     3601 ataaatcggc tcataaacgg ttccgtgctc cctgggctgc cagtagataa aatcattcag
     3661 cacaatatct tcaggaacag gtgaggttgt cacccctata ttatccacag gtttaacttc
     3721 tgttcctgta tgcactggtg ttaccgggac aggggaggta ttccctgtat cggtcgggac
     3781 agaaggtacc ggtaccggat taatcagaat ggtccgcgac gggccaccgt taattcccgg
     3841 aacaataatt ttatccagcc cagttagttc atcccgctcc gctttcagaa ctgggacaaa
     3901 cgggaaacgc cctgtagctc ccgtattaaa gaacctgatg acaggccgcc cattatccat
     3961 cactatagcc ccacggacag gaatgtgctc cattcgggaa aactcagaca tttgtatatc
     4021 ctctgccaga agacgggcat taacagcaaa taatgcctct acatcccgac cgggaacctt
     4081 atcactgttt tttcctgcct ccttcggcca taacccagca acagtaacgg aagcactcag
     4141 agttatcgga ttcactgcca tcagtgattt cacagcacta agtatccgcg tagcgatatt
     4201 tccggcaatt tcggccactg gtatccacgc ccctcttccg gcaacggata actgcattcc
     4261 ctgtggggca acccccataa ccaaagcacc tgctgccaac atgctacttt cagctttctt
     4321 caccatctca ggcgtataga ccggatctgt gacctcactg cccaaggcgc tactaggcca
     4381 ggggttaata tcaacaggag ggatctcgat cggcttaatc tccactgaat caatatcagc
     4441 aggtttaaaa tcagctaatt ccagctcgat tatcttaata ttttctagat caagcgcctg
     4501 aattgtcgga atctccggga tatgaatatc aggaggtggt aacacaggga tcaacagttc
     4561 tgctttcccc ggtagcgtat caggctttcc attattagaa ggctcaataa gatctggttt
     4621 ttctggagca gtaagtgagg ttcccgattc cagtcgtgat ttttcaactg aaagaatatc
     4681 cacctttttg gagacaatct catattcatg cagaagatga ttatatttcg taatcagttc
     4741 gttctgcgca tcaaacaatt ctttatttgg taatactacc atttctttcc atgaagtgat
     4801 actccagcgt gtatccggtc ttgatgcgag gccaataata aacgcccctt caaaataaga
     4861 gaaatcaccc aattgtttcg atttgtcaag aaattcctta tatttaaact tatccgtaaa
     4921 ttgattaagc atcgtttcaa cattatgtcg acgctcatca actaccacaa tcccggaatt
     4981 tacatcctca atactttttt caagacgact taatgctgta tccaactcat taaccaagac
     5041 agtaaaactg ctgtttccac gaacttcacc aagaagcgtt ttaacgctag atacaaattt
     5101 attaatatcg gaaggaagca atgacgtcag atgtttaatt gcactttcta acgcaactat
     5161 aatcttttta aggtcgctga ccgacgtaac ttctttgttg aaaatagaag gatcgaggaa
     5221 aataggttta tccaccggtg gtagtttatc aagaacacct gtattaatta ccggtttttc
     5281 tggaccgttg gtgataacaa taattggact accattaaaa tcataaggaa caccatcaac
     5341 gtaatgtgat atgggtgtcc cgctatatcc taaactattc gtcataataa aatccttttt
     5401 aaaatgcgca cacacaacaa ctgtacatgc atacagtacc attgtgctaa ttttgctcgc
     5461 cttgtcaaga aggctaattt ctttttttgt gataatcatc acaagcaagg gcttaaaacg
     5521 gatagatgag acctaatgac agcaactggc caaagcaaga gaaggggtca gtttggagat
     5581 cggaaacttt atgtacgtta agcgcattat ttaaccaatc tcgatgcgta acggattgat
     5641 tttgcgtcaa cgtttatcgc ttttgaggta ttgggggttt gagaaagggg tgatccgcta
     5701 cgctcacctc aaagacagca gccagggcgc tcagtatttc caaacttggc tgatcgccgt
     5761 tctcgatacg ctgcaccgtg cgcacgctta gcccacacag ttcgttaagc tgctcctggg
     5821 actaagtacg cgcaagacgc agtgacctaa attggtacat ggacatgtca gatttaccat
     5881 aatcccttat tgtacgcacc gttgaaacgc tccaggcgcg atcacggcaa cagacaggta
     5941 aaaatggcaa caaaccaccc gaaaaactgc cgcgatcgca catgataaat tttaacctga
     6001 tgaataccta tgcacccgga ttaattcccg actgtttcgc ggctttttct tcctgacgtg
     6061 ctcttttttc cgctttatcc ttcgcagcga aacgcctgat cagtttcagg gaatactccc
     6121 gctgttttgg cgtgacctcc ccggcaatct gaccatcaag acctgtgcgt tctttcatcg
     6181 tagttaaagc acgctggtac acataacgac gggtataaaa ttttattgcc gatgtcagga
     6241 gttccgaatc aaacggtaag cccctctgct ctgcatctgc aatcagctca gccagaatac
     6301 ccactttcag cggacgggga cggctgaaat taaacgcatc aggccagaat ccctccagca
     6361 acgccctgta ctcacgtcgt ttctgttctc tttcttcttg ttcggacaaa atcacctcca
     6421 cactcagcta ggcactgcta ttttacacat tcaccgcccc agcgacggac cacgccatac
     6481 ccgtacttcc cgttcctgct cttcatgcca ggaccgcgca cgctcccgtt ccaggcgtgc
     6541 ctgcctttcc tgttcatccc ttatctgctg ttcgtgataa ataaccgact caagcggtcc
     6601 acctgcccgg ctaatctctg cacctgctga ctcaagtcgt cgcactgttc cctcagttgc
     6661 ccgttctcct gtcgtgtcag ctcgaacata tgctgcaaat ccgtgaaggc gctctcccag
     6721 tctttcagcc gctgcgtata gtcctgctgc aattgctcta aggcgttcag taagtgcatt
     6781 tccagctctg tcatactcac ttactccctg accagtctta ctgcgttctt cttctccacc
     6841 gtccagttgt tttccccctt caccccggac ggcagcacca gaaatttccc gttcctgccc
     6901 tcgtgatacg tcacacccca tgttttttcc cggagtttcg ccagcgtctc ttcctggtcc
     6961 ctgatagcca ggatgttcgc cgcaatacgg ctttc
//
