LOCUS       NZ_CP075643             7768 bp    DNA     circular CON 27-JUN-2023
DEFINITION  Escherichia coli strain S19-141 plasmid pS19_141-c, complete
            sequence.
ACCESSION   NZ_CP075643
VERSION     NZ_CP075643.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993351
            Assembly: GCF_016404325.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 7768)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 0.4.9b
            Assembly Name                     :: S19-141_NP
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:11:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,151
            CDSs (total)                      :: 5,035
            Genes (coding)                    :: 4,825
            CDSs (with protein)               :: 4,825
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 90 of 210
            Pseudo Genes (incomplete)         :: 109 of 210
            Pseudo Genes (internal stop)      :: 63 of 210
            Pseudo Genes (multiple problems)  :: 49 of 210
            Pseudo Genes (short protein)      :: 5 of 210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075643.1.
            Bacteria and source DNA available from Institute Food Hygiene and
            Safety, University Zurich.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S19-141"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pS19_141-c"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2019"
                     /collected_by="ILS/NENT"
     gene            complement(join(7599..7768,1..22))
                     /locus_tag="JFX59_RS25425"
                     /old_locus_tag="JFX59_025425"
     CDS             complement(join(7599..7768,1..22))
                     /locus_tag="JFX59_RS25425"
                     /old_locus_tag="JFX59_025425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_097457174.1"
                     /translation="MQQDYTQRLKDWESAFTDLQHMFELTRQENGQLREQCDDLSQQVQ
                     RLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(71..631)
                     /locus_tag="JFX59_RS25430"
                     /old_locus_tag="JFX59_025430"
     CDS             complement(71..631)
                     /locus_tag="JFX59_RS25430"
                     /old_locus_tag="JFX59_025430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_074167501.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<663..1100)
                     /locus_tag="JFX59_RS25950"
                     /pseudo=""
     CDS             complement(<663..1100)
                     /locus_tag="JFX59_RS25950"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1309..1632)
                     /locus_tag="JFX59_RS25440"
                     /old_locus_tag="JFX59_025440"
     CDS             complement(1309..1632)
                     /locus_tag="JFX59_RS25440"
                     /old_locus_tag="JFX59_025440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1700..1891
                     /locus_tag="JFX59_RS25445"
                     /old_locus_tag="JFX59_025445"
     CDS             1700..1891
                     /locus_tag="JFX59_RS25445"
                     /old_locus_tag="JFX59_025445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165972.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2561..2704
                     /locus_tag="JFX59_RS25450"
                     /old_locus_tag="JFX59_025450"
     CDS             2561..2704
                     /locus_tag="JFX59_RS25450"
                     /old_locus_tag="JFX59_025450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(2899..3030)
                     /locus_tag="JFX59_RS25455"
                     /old_locus_tag="JFX59_025455"
     CDS             complement(2899..3030)
                     /locus_tag="JFX59_RS25455"
                     /old_locus_tag="JFX59_025455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            3060..3350
                     /gene="cki"
                     /locus_tag="JFX59_RS25460"
                     /old_locus_tag="JFX59_025460"
     CDS             3060..3350
                     /gene="cki"
                     /locus_tag="JFX59_RS25460"
                     /old_locus_tag="JFX59_025460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_074167500.1"
                     /translation="MHLKYYLHNLPESLTPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(3347..4819)
                     /locus_tag="JFX59_RS25465"
                     /old_locus_tag="JFX59_025465"
     CDS             complement(3347..4819)
                     /locus_tag="JFX59_RS25465"
                     /old_locus_tag="JFX59_025465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_086259340.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTDVQNAEIKLKEAKDDLEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFSSVDEALAAFEKYKNVLDKKISKVDRDAIFNALESVNYDELSKNLTKI
                     SKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGV
                     PLGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="colicin-like pore-forming protein"
     gene            5064..5354
                     /locus_tag="JFX59_RS25470"
                     /old_locus_tag="JFX59_025470"
     CDS             5064..5354
                     /locus_tag="JFX59_RS25470"
                     /old_locus_tag="JFX59_025470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063106837.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVATGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNDKKVMEKTGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(5346..5624)
                     /locus_tag="JFX59_RS25475"
                     /old_locus_tag="JFX59_025475"
     CDS             complement(5346..5624)
                     /locus_tag="JFX59_RS25475"
                     /old_locus_tag="JFX59_025475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063106834.1"
                     /translation="MKKCVPVLLFSVLSVSVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            5746..6168
                     /locus_tag="JFX59_RS25480"
                     /old_locus_tag="JFX59_025480"
     CDS             5746..6168
                     /locus_tag="JFX59_RS25480"
                     /old_locus_tag="JFX59_025480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_032319274.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6227..6493)
                     /locus_tag="JFX59_RS25485"
                     /old_locus_tag="JFX59_025485"
     CDS             complement(6227..6493)
                     /locus_tag="JFX59_RS25485"
                     /old_locus_tag="JFX59_025485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262325.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRKSGR
                     PLKGELGEFWRYRVGDYRILCEIRDDELIILVATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6477..6701)
                     /locus_tag="JFX59_RS25490"
                     /old_locus_tag="JFX59_025490"
     CDS             complement(6477..6701)
                     /locus_tag="JFX59_RS25490"
                     /old_locus_tag="JFX59_025490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879770.1"
                     /translation="MLAIRLSDEIESRLDSLAKKTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            6855..7067
                     /locus_tag="JFX59_RS25495"
                     /old_locus_tag="JFX59_025495"
     CDS             6855..7067
                     /locus_tag="JFX59_RS25495"
                     /old_locus_tag="JFX59_025495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000350688.1"
                     /translation="MDIEMNNTEEMFSKLGMRLGAEMAIPLKDGDNFAPTGCIVTKLDK
                     ENGLLICRPVQKEINGTWYTVDLSK"
                     /name="hypothetical protein"
     Typing          complement(1977..2064)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          2087..2633
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.192"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(37..1319)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(1692..1894)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.78e-11"
ORIGIN
        1 gctgcgtata gtcctgctgc aattgctcta aggcgttcag taagtgcatt tccagctctg
       61 tcatactcac ttactccctg accagtctta ctgcgttctt cttctccacc gtccagttgt
      121 tttccccctt caccccggac ggcagcacca gaaatttccc gttcctgccc tcgtgatacg
      181 tcacacccca tgttttttcc cggagtttcg ccagcgtctc ttcctggtcc ctgatagcca
      241 ggatgttcgc cgcaatacgg ctttcctgcc actgaatcag cccccatgac gccagaaaaa
      301 tcccgcccgt gacgcaaagc gccgtcagcg acgggtacag tatccgccct ttgatcagct
      361 tccagagcag ctcttcctgc cgccgggcca gttcgttctc tgtggcgctg aactgcgcgt
      421 tcacggcact gttcagcgtc tccagttgtt ctttcaccgc tgctgtgtgt gcgctgatag
      481 cgtctctgat tttctgcccg tttaagttca gttccctgtc tacagacgct tcgagcttcc
      541 tgaactcgct gttcagcatg ttctctgtag agacggcacg ctctttcagt ttcttctcga
      601 agtctgtccc catttgtaaa agattgctca tacagcgccc ctttcagcct gagattacgc
      661 ccgccctccg ggtcggcgat actgatactg ctcctggttg tcctcaccac ctcaaaacct
      721 gccgctgtaa gcgcctcagt gacatcctga cgcgttttta gcgctccggc atggtaaaga
      781 gcctccagtc ccctcgtaat cgcttctgcg gcctcctgtt tcgctttcgg cagattattc
      841 ggggtgacaa gtgtccgcct gttctccggt gcgttcgggt cgtgcagccc gtaatggtga
      901 ttcaccagtg tctgccaggc attgattcgc ggacggtccg cccggtcgta atagggctgg
      961 agccgttttc cgctcgccag ctccatattc gggatgacaa aattcagctc aagacgcccc
     1021 ttgtcctggt gctccaccca caggatgctg tactgatttt tttcaagacc gggcatcagt
     1081 acccgctcaa agctctccat caccctttca cgctctcccg gtggcagggt ctgctctgca
     1141 aaagacagaa cccccgaggt gtattttttc gcaaacggcg tcgcatcgat gagttcccgt
     1201 acctcttcgg gagcaccccg cagaactctc gccccttccc ggttacgctc ccggcccagc
     1261 aggtaatcaa ccggaccact gccaccgcct tttcccctgg catgaaactt aactatcatc
     1321 ccgttctccc tgtttacgga cctcatccct cagctcactc agttcacgtc cgatggccat
     1381 cagtgcagcc accacatgaa cccggtcatg ccccgaccac tgtccgctgt ttatcttccg
     1441 ggctatctga ttcaggttat tgccgaccga agcgaactgg cgcaacagcg gcggtgccag
     1501 tgtcggaaga cctgacgttt tcgatggcgg tgcccccagg cagaccttac gcatccatga
     1561 cgcaagttgt tttccctcac aacgtgccag cagccgcgca tgttcctcat ccgtgacccg
     1621 tatcgtgagc atcctttcgc gtttcaccgg tatcattaaa aacctccgac agactcccca
     1681 cacatggaga aacagaactg tgactaaaca ggaaaaaacc gcccttaaca tggcccgctt
     1741 catcaaaagc cagacgctga ccctgctgga aaaactgaat gaactggacg ccgacgacca
     1801 ggctgacatc tgcgaagcgc ttcacgacca cgccgacgag ctttaccgca gctgcctcgc
     1861 acgcttcggg gatgacggtg aaaacctctg acacatgcag ctcccggaga cgggcacagc
     1921 ttacctgtga gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cggtttttag
     1981 cgggtgtcgg ggcgcagccc tgacccagtc acgtagcgat agcggagtgt atactggctt
     2041 aatcgtgtgg catcagtgag gattgtatga aaagtgcacc atgtacggtg tgaaatgccg
     2101 cacagatgcg taaggagaac atgcagatgc cgatgctctt ccgcttcctc gctcactgac
     2161 tcgctgcgct cggtcgttcg gctgcggcga gcggtgtctg ctcacacaaa agcggcaata
     2221 ctgttatcca cagaatcagg ggataacgcc ggaaagaaca tgtgagcaac aagacaaagc
     2281 cggggaaaag gccgcgcagg tggcgctttt tccataggct ccgcccccct gacgagcatc
     2341 acaaaaaccg acgctcaagt cagaggtggc gaaacccgac aggacttaaa gataccaggc
     2401 gtttcccccc ggaagctccc tcgtgcgctc tcctgttccg accctgccga ttaccggata
     2461 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta
     2521 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt atgcacgaac cccccgttca
     2581 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2641 cttaacgcca ctggcagcag ccattggtaa ctggatagtg gatttagata cgcagaactc
     2701 ttgaagttga agccttatag cggctacact ggaaggacag catttggtat ctgtgctcca
     2761 ctaaagccag ttacccggtt aagcagtccc caactgactt aaccttcgac taaaccgcct
     2821 ccccaggcgg ttttttcgtt tacaggcagc agattacgcg cagaaaaaaa ggatctcaag
     2881 aagatccttt gatcttttct actgaaccgc gatccccgtc agttcagaag acgaagatgg
     2941 tgcaacggtt cctccttgca catcacgaat atagtttgct tgacatcctg acaagaataa
     3001 tattgtaatt agaaaaatta ttattttcat atttctttcc aacaaaagta aaaatactta
     3061 tgcatttaaa atactaccta cataatttac ctgaatcact tacaccatgg attcttattt
     3121 taatatttaa cgacaatgat aacactcctt tgttatttat atttatatca tcaatacatg
     3181 tattgctata tccatactct aaattaacca tatctagata tatcaaagaa aatacaaagt
     3241 taaaaaaaga accctggtac ttatgcaagt tatctgcatt gttttattta ttaatggcaa
     3301 tcccagtagg attgccaagt ttcatatatt acactctaaa gagaaattaa atccctaaca
     3361 actcattaag tttgttcaac tcatcatccc ctatgtaaga agaaacaata cctgtgacaa
     3421 ttgcaatccc ccagaaacca agaggcacac caacaataaa actaaacatt aatgccacaa
     3481 tttttgccac acctacatcg accgcacttt tctccagcgt gacaaacaaa ggtcgccagt
     3541 taccagtttc tattgcattt ttaaaatcag aaccaacatc atatagaaaa gatactcgac
     3601 tagtgatttt aagcgacttt gatatcttcg ttaaattctt agataactca tcataattaa
     3661 cagactctaa cgcattaaaa atagcatccc gatcaacctt actgattttc ttatccagta
     3721 catttttata cttttcaaat gcagcaagag cttcatcaac actactaaat ttactaccct
     3781 tcgccttttc agctaaatcc tgagctattc tggaatattt ttccccatat tgttcggtta
     3841 tatattgata aaacccaacc atagtatcaa cagagtcttt tacctgactc ttctcaagat
     3901 catccttagc ttcttttaat tttatctcag cattctgaac atctgttttt ttagcatctc
     3961 taatgctaac cgcagaatta agagcattcg tcactgacga atattcattc ttcttatcct
     4021 gcaattgtct ctccagtttt ttctgaacat cacgccatcc aggaaatcca gtaaccttca
     4081 catcaacact tttttgagcg ttttcaagct cccctgcaac tctggaaaca atcgcttgct
     4141 tactctgtac atcgctttcg gccttcgcca gttcagcttt agcctcagcc agcttcttct
     4201 ctgccacttc cacagcccga tgctcttcat caagagaatc ctgtcgattt ttctcagcct
     4261 ctgctgcctc cgccattgct aacaaatgcg cggtttcagc ctgttggcgg gcaatctcat
     4321 cacgttggcg ttctgcttcc cggagcgctt tttctgctgc ttcagcttct tcacgggctt
     4381 tttgctctgc cttcgcaagg cgcaaacgct cagcctctgc ctgcattgcc atattattgg
     4441 catgagcaag gtcagttact gatggactac gggcggcatt agcacgtaaa gcgtcattaa
     4501 caatatcctt gagacgttga gtcagggcat cacgctgaga tttcgctttt gccaatgctg
     4561 cctcagccgc agcagcacgg gcagcctgtt cagcctgatg ttttttcaac tgcgcggtag
     4621 accatttagc ggttgcatgt atcgcagcag aactttcact ggccccacca aatgctccac
     4681 cgcctccacc aacattttca gttccagttg caagcgtcgc tgtaatcgta tcgccagtat
     4741 caaccccaac agttgggaat gacccctcag tagattctgt gctctccaca tctggagaat
     4801 tatcagtgac tttatccata aagaaattcc tctttgataa ttaaaacaat aaattaaaaa
     4861 caacatactg tacatataac cactggtttt atgtacagtg aaaacctact actcagcatt
     4921 gtccatgtca agagcatgga ttttcatttt tgcaataagg atcacactat ggggaggcag
     4981 gcattgagaa cgtcgaaaca gaacaccgga gcaaatcagg atgagatata aaactgttgg
     5041 atcatgaaaa aacggagaac gatgtgagca aatcaccccg ccataaactg aacaaaacag
     5101 acaaacgact tctcgacacc cttgttgcta ccggatatga gcatgacaaa gcccgtgacc
     5161 tcatccagaa acaggtttac acgctgacac tggcagatca gcgtcatgtg gtcagtgaaa
     5221 tcagtaatgg tgtgaatccc acccaggctt actcggcggt ataccaggca agacgcattc
     5281 gcctcgcccg taaatatctg aacgataaaa aggttatgga aaaaaccggg gaaaatacgc
     5341 ccccatcagc gtaaggattt cttttgccgc tccagagact ccagtttttt acgcaaatcc
     5401 tctctttttt gggcatctct ggtgccagcc agctctgctc tcaactcatc gatctgaagt
     5461 tgtatcttca gtctgttact gaacattttc tgtctggcat taacatccgc aacaatgccg
     5521 ttttttgtct tctcggcctt ttgttgaaaa acattgctgt ccgcatgact ggcaaccgaa
     5581 acagaaagaa cactaaaaag caggactggc acacattttt tcacgggatt attcctgact
     5641 cattgaccat caaatcacat tgggagtaaa ccgacgtatg ataagagata ctcttcggag
     5701 atataactcc ctgagtatca agattaaaaa cgcaaggaga tgtttatgag atctgccgct
     5761 gccaggctgc ttctgatacc tctgataaca gcaacaatag ctcttacagg atgcacacca
     5821 aagaccagcc tggaacgaca tacccggcat tatgtttatg cttcagatga tggatttgat
     5881 cctaacttct acacccagaa agcagacacc atacgtatga tgctcccgtt ctttcagcag
     5941 ttccgggata tggggatgaa agacaaagca gccggagtat cagcagaaac ggcacagcaa
     6001 cgtgtaaaag aattccactc agaaaaattt tttcactcac tccagagcac aacaaccttt
     6061 gctggcagaa aatacacaaa cagcgatatg ccttcgccga aaaaaatgaa actaatggca
     6121 gacaccattt ctgcggttta tctcgatgga tacgagggca gacagtaagg gatttatcat
     6181 aatcccttat tgtacgccac atatgaaaaa gcccgcatgg cgggcttcag tcgtagactt
     6241 cacgtcgatg gccgatggtg gccacaagaa taatcagttc gtcatccctg atttcacaca
     6301 gaatgcgata atcacccaca cgataacgcc agaattctcc caactccccc ttcagtggcc
     6361 tccctgattt acggggatcg gctgcaactg caatacgtaa atccataaaa tccacaatcc
     6421 gtcgcgcgtt ctgcttgtcc atcttacgta gcgatttgag cgcccgatcg gaataattaa
     6481 tcgtccagac caagagactt ccttacgtcc tcagacgaat gcacagcctc atcaccacgg
     6541 cgaacacgtg ctgctgtttc cgctgaaaga taataatctt ccagatcttc caggtgcgcc
     6601 agtatcgctt cccgggcata gaacgtcttt gtcctcccgg ttttcttcgc cagcgagtcc
     6661 agacgggact caatttcatc agacaaccga atagccagca tacaaatctc cctccgaaca
     6721 attgttatac atgtatagca aaaattataa cgtaaaaaga cgataaaaca atcaaaggct
     6781 ggttgaataa acagtaaaat cccatatatt aacgactgat atatgataag tatcggtttt
     6841 aaaaggaaaa caacatggat attgaaatga ataacactga agagatgttc agcaaactgg
     6901 ggatgcggtt aggagctgag atggcaatcc ctttgaagga tggtgataat tttgcgccaa
     6961 ctggatgcat cgttacgaag cttgataaag agaatggcct tctgatctgc cgaccagttc
     7021 agaaggaaat caacggcacg tggtacaccg tcgatttaag taagtaacac cacggactaa
     7081 agccctgcgg ggctttttta tacctgccgc tgactctgat tgaacataac atctgaattg
     7141 tacgcaccgc tgaaacgcgt tcatcgcgat cacggcagca gacaggtaaa aatggcaaca
     7201 aaccacccga aaagctgccg cgatcgcacc tgataaattt taaccgtatg catagctatt
     7261 cagccatgtg aataacgctg gttttgcctg cgtaaacctc atgacactgt tttttttcca
     7321 tcttttcagt tgatgacata cgcagacatc gcgggatgag gctgaggaat gagcgcgatc
     7381 tggcaaagag gcaaaacaca gcaacaaaaa cgacacgcca gaatcgcgcc cggatgcgtt
     7441 tttaacgcgt tccggtacca tctggcaacc tcccggaaca actcaccgtc acatccccat
     7501 tgacgggcca cgccataccc gtacttcccg ttcctgctct tcatgccagg accgcgcacg
     7561 ctcccgttcc aggcgtgcct gcctttcctg ttcatccctt atctgctgtt cgtgataaat
     7621 aaccgactca agcggtccac ctgcccggct aatctctgca cctgctgact caagtcgtcg
     7681 cactgttccc tcagttgccc gttctcctgt cgtgtcagct cgaacatatg ctgcaaatcc
     7741 gtgaaggcgc tctcccagtc tttcagcc
//
