LOCUS       NZ_CP075639             5936 bp    DNA     linear   CON 27-JUN-2023
DEFINITION  Escherichia coli strain 2018-166 plasmid pSTEC2018_166-b.
ACCESSION   NZ_CP075639
VERSION     NZ_CP075639.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16993346
            Assembly: GCF_016404485.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Distribution of virulence factors, antimicrobial resistance genes
            and phylogenetic relatedness among Shiga toxin-producing Escherichia
            coli serogroup O91 from human infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Nusch-Inderbinnen,M., Stevens,M.J.A., Cernela,N., Muller,A.,
            Biggel,M. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
REFERENCE   3  (bases 1 to 5936)
  AUTHORS   Stevens,M.J.A., Stephan,R. and Nusch-Inderbinnen,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method :: unicycler v. 0.4.9b; SPAdes v. 3.15.2
            Assembly Name                     :: 2018-166_NP
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Oxford Nanopore; Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/27/2023 06:22:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,465
            CDSs (total)                      :: 5,343
            Genes (coding)                    :: 5,049
            CDSs (with protein)               :: 5,049
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 133 of 294
            Pseudo Genes (incomplete)         :: 162 of 294
            Pseudo Genes (internal stop)      :: 52 of 294
            Pseudo Genes (multiple problems)  :: 50 of 294
            Pseudo Genes (short protein)      :: 4 of 294
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075639.1.
            Bacteria and source DNA available from ILS.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2018-166"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC2018_166-b"
                     /geo_loc_name="Switzerland: Zurich"
                     /lat_lon="47.40 N 8.55 E"
                     /collection_date="2018"
                     /collected_by="ILS/NENT"
     gene            complement(215..616)
                     /locus_tag="JFX80_RS27070"
                     /old_locus_tag="JFX80_27090"
     CDS             complement(215..616)
                     /locus_tag="JFX80_RS27070"
                     /old_locus_tag="JFX80_27090"
                     /inference="COORDINATES: protein motif:HMM:NF016255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_052074460.1"
                     /translation="MSEQEEREQKRREYRALLEGFWPDAFNFSRPRPLKVGILAELIAD
                     AEQRGLPFDSELLTSAIKFYTRRYVYQRALTTMKERTGLDGQIAGEVTPKQREYSLKLI
                     RRFAAKDKAEKRARQEEKAAKQSGINPGA"
                     /name="ProQ/FINO family protein"
     gene            complement(768..1001)
                     /locus_tag="JFX80_RS27075"
                     /old_locus_tag="JFX80_27095"
     CDS             complement(768..1001)
                     /locus_tag="JFX80_RS27075"
                     /old_locus_tag="JFX80_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1008..1550)
                     /locus_tag="JFX80_RS27080"
                     /old_locus_tag="JFX80_27100"
     CDS             complement(1008..1550)
                     /locus_tag="JFX80_RS27080"
                     /old_locus_tag="JFX80_27100"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000536290.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTGG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1600..2037)
                     /locus_tag="JFX80_RS27510"
                     /pseudo=""
     CDS             complement(<1600..2037)
                     /locus_tag="JFX80_RS27510"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2246..2569)
                     /locus_tag="JFX80_RS27090"
                     /old_locus_tag="JFX80_27110"
     CDS             complement(2246..2569)
                     /locus_tag="JFX80_RS27090"
                     /old_locus_tag="JFX80_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2637..2828
                     /locus_tag="JFX80_RS27095"
                     /old_locus_tag="JFX80_27115"
     CDS             2637..2828
                     /locus_tag="JFX80_RS27095"
                     /old_locus_tag="JFX80_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3899..4030)
                     /gene="ctl"
                     /locus_tag="JFX80_RS27105"
                     /old_locus_tag="JFX80_27125"
     CDS             complement(3899..4030)
                     /gene="ctl"
                     /locus_tag="JFX80_RS27105"
                     /old_locus_tag="JFX80_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 lysis protein"
                     /protein_id="WP_072023375.1"
                     /translation="MKITFLIITILSLSGCQANYISDVQGGTVAPSSSSELTGIAVQ"
                     /name="ctl"
     gene            4055..4345
                     /gene="cti"
                     /locus_tag="JFX80_RS27110"
                     /old_locus_tag="JFX80_27130"
     CDS             4055..4345
                     /gene="cti"
                     /locus_tag="JFX80_RS27110"
                     /old_locus_tag="JFX80_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 immunity protein"
                     /protein_id="WP_072023376.1"
                     /translation="MTVKYYLHNLLESLIPWLFYLLLNYKTPPFSLFIFIASIHVLLYP
                     YSKLTIFSFIQNTTKMKKEPWYSYNLFYFLYLAMAIPVGLPSFIYYSLKRN"
                     /name="cti"
     gene            complement(4342..5814)
                     /gene="cta"
                     /locus_tag="JFX80_RS27115"
                     /old_locus_tag="JFX80_27135"
     CDS             complement(4342..5814)
                     /gene="cta"
                     /locus_tag="JFX80_RS27115"
                     /old_locus_tag="JFX80_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin 10"
                     /protein_id="WP_033801039.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTEVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFNSVDEALAAFEKYKNVLDKKFSKVDRDDIFNALESITYDEWAKHLEKI
                     SRALKVTGYLSFGHDVWDGTLKGLKTGDWKPLFVTLEKSAVDFGVAKIVALMFSFIVGA
                     PLGFWGIAIITGIVSSYIGDDELNKLNELLGI"
                     /name="cta"
     Typing          complement(2915..3002)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="2.44e-33"
     Typing          3229..3771
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.18"
                     /coverage="93.0"
                     /evalue="2.61e-106"
     Typing          complement(665..2256)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.247"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2629..2831)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.059"
                     /coverage="100.0"
                     /evalue="2.24e-14"
ORIGIN
        1 cgccgaaaaa aatgaaacta atggcagaca ccatttctgc ggtttatctc gatggatacg
       61 agggcagaca gtaagggatt taccataatc ccttaattgt acgcaccgtt gaaacgctcc
      121 aggcgcgatc acggcaacag acaggtaaaa atggcaacaa accacccgaa aaactgccgc
      181 gatcgcacat gataaatttt aacctgatga atacctatgc acccggatta attcccgact
      241 gtttcgcggc tttttcttcc tgacgtgctc ttttttccgc tttatccttc gcagcgaaac
      301 gcctgatcag tttcagggaa tactcccgct gttttggcgt gacctccccg gcaatctgac
      361 catcaagacc tgtgcgttct ttcatcgtag ttaaagcacg ctggtacaca taacgacggg
      421 tataaaattt tattgccgat gtcaggagtt ccgaatcaaa cggtaagccc ctctgctctg
      481 catctgcaat cagctcagcc agaataccca ctttcagcgg acggggacgg ctgaaattaa
      541 acgcatcagg ccagaatccc tccagcaacg ccctgtactc acgtcgtttc tgttctcttt
      601 cttcttgttc ggacaaaatc acctccacac tcagctaggc actgctattt tacacattca
      661 ccgccccagc gacggaccac gccatacccg tacttcccgt tcctgctctt catgccagga
      721 ccgcgcacgc tcccgttcca ggcgtgcctg cctttcctgt tcatccctta tctgctgttc
      781 gtgataaata accgactcaa gcggtccacc tgcccggcta atctctgcac ctgctgactc
      841 aagtcgtcgc actgttccct cagttgcccg ttctcctgtc gtgtcagctc gaacatatgc
      901 tgcaaatccg tgaaggcgct ctcccagtct ttcagccgct gcgtatagtc ctgctgcaat
      961 tgctctaagg cgttcagtaa gtgcatttcc agctctgtca tactcactta ctccctgacc
     1021 agtcttactg cgttcttctt ctccaccgtc cagttgtttt cccccttcac cccggacggc
     1081 agcaccagaa atttcccgtt cctgccctcg tgatacgtca caccccatgt tttttcccgg
     1141 agtttcgcca gcgtctcttc ctggtccctg atagccagga tgttcgccgc aatccggctt
     1201 tcctgccact gaatcagccc ccatgacgcc agaaaaatcc cgcccgtgac gcaaagcgcc
     1261 gtcagcgacg ggtacagtat ccgccctttg accagcttcc agagcagctc ttcctgccgc
     1321 cgggccagtt cgttctctgt ggcgctgaac tgcgcgttca cggcactgtt cagcgtctcc
     1381 agttgttctt tcaccgctgc tgtgtgtgcg ctgatagcgt ctctgatttt ctgcccgttt
     1441 aagttcagtt ccctgtctac agacgcttcg agcttcctga actcgctgtt cagcatgttc
     1501 tctgtagaga cggcacgctc tttcagtttc ttctcgaagt ctgtccccat ttgtaaaaga
     1561 ttgctcatac agcgcccctt tcagcctgag attacgccca ccctccgggt cggcgatact
     1621 gatactgctc ctggttgtcc tcacaacctc aaaacctgcc gctgtaagcg cctcagtgac
     1681 atcctgacgc gtttttagcg ctccggcatg gtaaagagcc tccagtcccc tcgtaatcgc
     1741 ttctgcggcc tcctgtttcg ctttcggcag attattcggg gtgacaagtg tccgcctgtt
     1801 ctccggtgcg ttcgggtcgt gcagcccgta atggtgattc accagtgtct gccaggcatt
     1861 gattcgcgga cggtccgccc ggtcgtaata gggctggagc cgttttccgc tcgccagctc
     1921 catattcggg atgacaaaat tcagctcaag acgccccttg tcctggtgct ccacccacag
     1981 gatgctgtac tgattttttt caagaccggg catcagtacc cgctcaaagc tctccatcac
     2041 cctttcacgc tctcccggtg gcagggtctg ctctgcaaaa gacagaaccc ccgaggtgta
     2101 ttttttcgca aacggcgtcg catcgatgag ttcccgcacc tcttcgggag caccccgcag
     2161 aactctcgcc ccttcccggt tacgctcccg gcccagcagg taatcaaccg gaccactgcc
     2221 accgcctttt cccctggcat gaaacttaac tatcatcccg ttctccctgt ttacggacct
     2281 catccctcag ctcactcagt tcacgtccga tggccatcag tgcagccacc acatgaaccc
     2341 ggtcatgccc cgaccactgt ccgctgttta tcttccgggc tatctgattc aggttattgc
     2401 cgaccgaagc gaactggcgc aacagcggcg gtgccagtgt cggaagacct gacgttttcg
     2461 atggcggtgc acccaggcag accttacgca tccatgacgc aagttgtttt ccctcacaac
     2521 gtgccagcag ccgcgcatgt tcctcatccg tgacccgtat agtgagcatc ctttcgcgtt
     2581 tcaccggtat cattaaaaac ctccgacaga ctccccacac atggagaaac agaactgtga
     2641 ctaaacagga aaaaacagcc cttaacatgg cccgctttat cagaagccag acgctgacac
     2701 tgctggaaaa actgaatgaa ctggacgccg acgaccaggc ggacatctgc gaagcccttc
     2761 acgaccacgc cgacgagctt taccgcagct gcctcgcacg cttcggggat gacggtgaaa
     2821 acctctgaca catacagctc ccggagacgg tcacagcttg cctgtgagcg gatgccggga
     2881 gcagacaagc ccgtcagggc gcgtcagcag gttttagcgg gtgtcggggc gcagccatga
     2941 cccagtcacg tagcgatagc ggagtgtata ctggcttata ccatgcagaa tcagtgcgga
     3001 ttgtatgaaa agtgcatcat gccgagtgtg aaatgccgca cagatgcgta aggagaaaat
     3061 gcagcttccg gtgctcttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     3121 tgcggcgagc ggactcccct ttctcaaagg cggtaacccc gttatccaca gaatccgggg
     3181 ataacccggg aaagaacatg tgcgcaaaaa acgaagaccc cgggaaaggc cgcggcacag
     3241 gcgctttttc cataggctcc gcccccctga cggacttcac aaaacagaca ctttttcggt
     3301 gcatctgtgg gagccttacg gctcaaccat gaaatccgcc agcggtggcg aaacccgacg
     3361 tggcttaaag atcatcaccg tttccggtga atcgctccct cctgcgcccc cacgttcccg
     3421 ccatatcgat taccggatac cttttccgcc tttctccctt gcgggacacg tggcgctttc
     3481 tcatagctca cgtcttctgg tatctcgggg cgacttaggc agttcgctcc gggccgggct
     3541 gtatgcagga accccccgtt cagcccgact gctgcgcctt ctccggtaac tgttctcttg
     3601 ggtccaaccc ggaaaggaca cgacttaacg ccactggcag cagccattgg taaccaggtt
     3661 agcagagcga gatttataag ttagtaacag agttcttgaa gtatcagcct gattttgact
     3721 acactagaag gacaagtttt gggtatctgc gctctgctga agccagttac cttcggaaaa
     3781 agagttggta gatcttgatc cggcaaacaa accaccgttg gtagcggtgg tttttttgtt
     3841 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3901 actgaaccgc gatccctgtc agttcagaag aggaagatgg agcgaccgtc cctccttgta
     3961 catcactaat ataatttgct tgacatcccg acaaggataa tattgtaatt atcaaaaacg
     4021 ttattttcat tctaccccta taacaacata aataatgacc gtaaaatact acctacacaa
     4081 tttactagag tcacttatcc catggctatt ctatttgtta ttaaactata agacgccccc
     4141 attttcactt ttcatattca tcgcatctat acatgtatta ctatacccat attcaaaact
     4201 aactatattt tcttttatac aaaacacgac aaaaatgaaa aaagaacctt ggtactcata
     4261 caaccttttt tattttcttt acctagcaat ggcaatccca gtaggattgc caagtttcat
     4321 atattactct ctaaagagaa attaaatacc tagcaattca ttaagcttgt tcaactcatc
     4381 atcccctatg taagaagaaa caatacctgt gataattgca attccccaga agccaagagg
     4441 cgcaccaaca ataaaactaa acattaatgc cacaattttt gccacgccga aatctaccgc
     4501 gctcttctcc agagtgacaa ataaaggctt ccagtctcct gtttttaatc cctttagggt
     4561 accatcccat acatcatgcc cgaaagacaa atatccagta accttaagag ctctagagat
     4621 cttttctaga tgcttggccc actcatcata agtaatagac tctaaagcat taaaaatatc
     4681 atccctatca accttactga atttcttatc cagtacattt ttatactttt caaatgcagc
     4741 aagtgcttca tcaacactat taaatttact acccttcgcc ttttcagcta aatcctgagc
     4801 tattctggaa tatttttccc catattgttc ggttatatat tgataaaacc caaccatagt
     4861 atcaacagag tcttttacct gactcttctc aagagcatcc ttagcttctt ttaattttat
     4921 ctcagcattc tgaacttctg tttttttagc atctctaatg ctaacagcag aattaagagc
     4981 attcgtcact gacgaatatt cattcttctt atcctgcaat tgtctctcca gttttttctg
     5041 aacatcacgc catccaggaa atccggtaac cttcacatca acactttttt gagcgttttc
     5101 aagctcccct gcaactctgg aaacaatcgc ttgcttactc tgtacatcgc tttcggcctt
     5161 cgccagttca gctttagcct cagccagctt cttctctgcc acttccacag cccgatgctc
     5221 ttcatcaaga gaatcctgtc gatttttctc agcctctgct gcctccgcca ttgctaacaa
     5281 atgcgcggtt tcagcctgtt ggcgggcaat ctcatcacgt tggcgttctg cttcccggag
     5341 cgctttttct gctgcttcag cttcttcacg ggctttttgc tctgccttcg caaggcgcaa
     5401 acgctcagcc tctgcctgca ttgccatatt attggcatga gcaaggtcag ttactgatgg
     5461 actacgggcg gcattagcac gtaaagcgtc attaacaata tccttgagac gttgagtcag
     5521 ggcatcacgc tgagatttcg cttttgccaa tgctgcctca gccgcagcag cacgggcagc
     5581 ctgttcagcc tgatgttttt tcaactgcgc ggtagaccat ttagcggttg catgtatcgc
     5641 agcagaactt tcactggccc caccaaatgc tccaccgcct ccaccaacat tttcagttcc
     5701 agttgcaagc gtcgctgtaa tcgtatcgcc agtatcaacc ccaacagttg ggaatgaccc
     5761 ctcagtagat tctgtgctct ccacatctgg agaattatca gtgactttat ccataaagaa
     5821 attcctcttt gataattaaa acaataaatt aaaaacaaca tactgtacat ataaccactg
     5881 gttttatgta cagcaaaacc tactactcag cattgtccat gtcaagagca tggatt
//
