LOCUS       NZ_CP075609             4375 bp    DNA     circular CON 13-SEP-2023
DEFINITION  Enterococcus faecalis strain 1207/14 plasmid p1207_5, complete
            sequence.
ACCESSION   NZ_CP075609
VERSION     NZ_CP075609.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19292310
            Assembly: GCF_018986755.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     VanA Enterococcus faecalis in Poland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) Molecular Microbiology, National Medicines
            Institute, Chelmska 30/34, Warsaw 00-725, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/13/2023 03:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,539
            CDSs (total)                      :: 3,453
            Genes (coding)                    :: 3,367
            CDSs (with protein)               :: 3,367
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 39 of 86
            Pseudo Genes (incomplete)         :: 38 of 86
            Pseudo Genes (internal stop)      :: 22 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075609.1.
            Bacteria and source DNA available from National Medicines
            Institute, Poland.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="1207/14"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="p1207_5"
                     /geo_loc_name="Poland: Ostrow Mazowiecka"
                     /lat_lon="52.8071 N 21.8796 E"
                     /collection_date="2014"
                     /collected_by="National Medicines Institute"
     gene            join(4059..4375,1..1090)
                     /locus_tag="KKP64_RS17445"
                     /old_locus_tag="KKP64_17455"
     CDS             join(4059..4375,1..1090)
                     /locus_tag="KKP64_RS17445"
                     /old_locus_tag="KKP64_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002333830.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDENI
                     YLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGTL
                     DDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDEVSP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIERLMA
                     DFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENEREK
                     ALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            1539..2513
                     /locus_tag="KKP64_RS17450"
                     /old_locus_tag="KKP64_17460"
     CDS             1539..2513
                     /locus_tag="KKP64_RS17450"
                     /old_locus_tag="KKP64_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_010727957.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVGN
                     LKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKWQE
                     GKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQLRRG
                     KATDWHAMVQESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYKYRNLL
                     KQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            2510..2743
                     /locus_tag="KKP64_RS17455"
                     /old_locus_tag="KKP64_17465"
     CDS             2510..2743
                     /locus_tag="KKP64_RS17455"
                     /old_locus_tag="KKP64_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303408.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
                     /name="hypothetical protein"
     gene            2736..3155
                     /locus_tag="KKP64_RS17460"
                     /old_locus_tag="KKP64_17470"
     CDS             2736..3155
                     /locus_tag="KKP64_RS17460"
                     /old_locus_tag="KKP64_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044384217.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGIIM
                     LDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            3164..3472
                     /locus_tag="KKP64_RS17465"
                     /old_locus_tag="KKP64_17475"
     CDS             3164..3472
                     /locus_tag="KKP64_RS17465"
                     /old_locus_tag="KKP64_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002333831.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            3476..3838
                     /locus_tag="KKP64_RS17470"
                     /old_locus_tag="KKP64_17480"
     CDS             3476..3838
                     /locus_tag="KKP64_RS17470"
                     /old_locus_tag="KKP64_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     Typing          1618..2513
                     /name="rep_cluster_2146"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.443"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..1087
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.79"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4060..4373
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.048"
                     /coverage="95.0"
                     /evalue="3.05e-62"
ORIGIN
        1 ttttcgtact actcgtgatg atggttcttc tactggcata tcggaacaac aagcagaaca
       61 aaatcgagtg attgccaaca aaattttagt tgaatatttc aaagaattta aagaacggaa
      121 tcctaattta tccgtctaca atgctgttat tcataatgac gaagtaagtc cgcacctgca
      181 tttgaatatt gttcctgttg ctgaagggta taaacggggt gttcaaaagc aaccaagttt
      241 taacaaagcc ttgcaacagc agggtttacg agcagataaa gagaatggtc gagtgatttg
      301 gaataatttt aggaatcagg aagttgagtc aattgaacgt ttgatggcag attttggttg
      361 ggagcgtgag ttagtcggta ctaacaaaat caaagatgtt cacgaatata aagaaatcat
      421 gagcgagatt tcagaattgc gtcaaacggc cgtaaaagag cgtgagagcg tttctgatga
      481 gttgaggtat atttctaggc aaagagaaga aaatgagcga gaaaaggctc tggtggcttc
      541 tgagagagtg aagattgaag aagagaagaa aaatatgctg aatgtaaaaa aagagatggc
      601 ggtcattttc caaaaagata ttgttccttt agaaagtttc aagaggcttc ctgatggttc
      661 gtatcattta tctgggaatg attttgttga tttgtatcaa cgagctacga gagcaacaaa
      721 tgccaagaga aatgctgata acatgagtga gcaatattta cagatgcttg agcaaaatac
      781 agaattaaat gagtatgtag ctacattgga agaagagcgt catttgtctg gtagaaaaca
      841 attagcagat ttacaagtgg aaaataaaaa gctgaaagaa gaaaatcgct ctttacgcca
      901 gcgtttagag cgattaaaaa acgcatttca aaaatcgatc actcgtttgt ctatccgttt
      961 tggtcttgca gaaaaggata tggggctttc tgaggaatta gaagtgttag agcaggagaa
     1021 aaccttattt tatcgtcaaa acgtctcaca gcgtaaatta gagcgttctg aagatgagat
     1081 cgagtggtag ttacgatgtg cagtcaacgg taccggaggc ggtttgtgcc gaggataagg
     1141 agttgacggc taaaccacta atccctgaag ctgacatttg ctaaaaaatg gcaagtccat
     1201 tttttggcca acctagcgtt agcgaagttt gggggcgccc ttttcaaagg gtgcggtttt
     1261 attttttgga tatttgcata gctaaagaga agtgctatta ttgagttatc aaataaataa
     1321 actgaaaaaa gagcaaaaaa ataaagtcga gcaacgctgg acacgttctc gacctttacg
     1381 gaaactaatc gcactagctc cgtaacttct taatcattat acgaatattt ttagatttct
     1441 tcaagactag agattttttg gtaatgaatt tttaagagtt acgtgaactt ttgaaaattc
     1501 tttccagaag tctctttttc tttgaggagg aacaaaattt gagtaaaaaa ggcacgatca
     1561 taaatgagtg ggaaaaatta acagccccct ctaacaggcg gttaagtgtc ccttttatgg
     1621 acacccccct aaaaattcag tggtcaatcg accgaattac cattgttgga aatcttaaag
     1681 agaatatcta ttatcacact acacatgatg tgttgatttt agattttgaa cagcttatgc
     1741 gcctaaatga aggtaacggt tatttaagat ctgttagtaa taatggttgg cagttgttag
     1801 accaacatga agagaatatt gcttatattg aaattttaaa atggcaagag gggaaagggc
     1861 gtatagactt caatccgaat aagattagtc aattcttagc tagttccatg aaaaacttta
     1921 ttcatgattt gtttatcgaa cctcactttt cacgtgcaga tattgcttgc gacattatcg
     1981 atgtacctga tgagtttatt acacaatatc gggtagttga tccagtttct tttaagccta
     2041 tttatggacg gaatggaaaa ttagagacgg catattgggg atctcgctct tctgaacgtc
     2101 aaattcggat gtataataaa aaactggaac aagagaagaa aagaaaaatt gtgccaaaag
     2161 aaatcgtgag ttggtggcgg ttagaattac aattaagacg agggaaagcg actgattggc
     2221 atgcaatggt tcaagaaagc ttggatagtt ttgctagtcc tcattacttg cctgctgata
     2281 caagtgtagc tgacaaaatg atgattactg ctttaacaac agaacatgat tattgggggc
     2341 aaattaatcg aaagacaaaa tataaatatc gaaatttgct gaagcaagaa agtcaaaatg
     2401 atgaattgac gaatcattta cgagaaacct ttgcagaatc ggcagacgat ttaaaaaaag
     2461 aattagacac atggcttcta gggctagacg taacagagga ggaagaaaaa tgacagaaaa
     2521 acaaaagtat tttggtaaga aaaattggtt ggtaaattta acaggtgatt acgttgtggt
     2581 tgtttttttg gatagcaaaa aaatgtccgg taagttactt gatgtaacgc aatatgaatt
     2641 gattttgaaa aataataaaa ttgactctcc gattattatc cctaagcacg ccgttaaata
     2701 tatttatgcg gtagctgaaa gtagtggaga agctaatgac tgatttgtca gaaaagatga
     2761 ccataaaaaa tcttgctgac gaattgggtg tatcgaaaaa tacagtgcgc aatcaactgg
     2821 tcaattcagg gtttgacctt gctcagtata aagaaaatgg cattatcatg ctagataaag
     2881 agttgattgg tgttgttcgg tcaatttatc ggtcaaaaag tgatcaattg actggtcaat
     2941 taccggtcaa taacacaggc agtgattttc ctttagaaaa agagaatgag tttttgcgtg
     3001 atcaattagc tgagaaggac aaacatattt ctgaattaac agaaatgatt cgtgatcaac
     3061 aaaaattgac cctgcttcag caacaaaaaa acgatcagtt gttactagaa aatcaagaat
     3121 taaaaaatcg aaaatggtgg caattctgga agtgaggtgt gacatggata aaattgattt
     3181 gagagaagaa acagtcgata gaattgaatt gatgtatgaa cggttagctg attatttgaa
     3241 tttgccggat gaagataatt atagtgaaat gcttgaacga attatggatg ttctcaactc
     3301 ggttatcaat gctgatttag caaaatcttc agttttgact aatgaatttg ttcataagca
     3361 tttagcgcaa acaactgatg aatcagaaaa agaattttgg ttgaaattaa tgagtcatgt
     3421 tatgcagtta aaccaaattt ccgttattaa ttccaaagaa aaggagatgt agtttatggg
     3481 aatgacttta gcagatatta aagcgcaaga agaaaaatta aaaaaagaac aagcgaaact
     3541 tcgccaacag aaaaaacgct tgattgagaa agaaaatgct gcaatgggca aggctcttcg
     3601 gaagcgtttt gggtttgaaa cttttgctga ttttgagcgg tggctatcgt cgatttctga
     3661 cgatcagcaa cacgaaaaat cagtggataa tgagtggctt gctgatgaaa aatcagcgcc
     3721 aagcgaacga gattatcccg atgaatctga cggcttattc ggagtgatac cgaataagaa
     3781 aagtcagatg agtgagagta aatttacgtc aatatttaat gacgtgaatt tacgataagc
     3841 gattattttg cgtgaagcaa aataaagcgt aaaaggagtt gagagcgtga cacgctttcg
     3901 gctccttttt tcgttgtcac ttgtgacagc gaataccctc ggagagcatt tggaattgtg
     3961 acagccatgt cacaattagg aaaatgttac tctttgacaa agagtaacag aaaggtttac
     4021 actcttttta ttagcgacag ccgaaggagg gttttaactt ggggttcagt atttcgttca
     4081 aaaagggaac aaagaataca agcatttctc ataataatcg tgagttaact gatagccaaa
     4141 aaatcaatga ctggcataag catattgatt tcacaaaatc ggatgaaaat atctatttag
     4201 aacaaacaaa tattcgtgaa aaatatgaag aattattcgg tgaagccgta gaaaaatata
     4261 atgcaaagca gaaaagagcc gatagaaaaa tcgaggacta tttagcaaag gttcgcaagg
     4321 ataaaaaatt ggaaccacag cgagaattta tcgtgcaaat tggtactctt gatga
//
