LOCUS       NZ_CP075608            21607 bp    DNA     circular CON 13-SEP-2023
DEFINITION  Enterococcus faecalis strain 1207/14 plasmid p1207_4, complete
            sequence.
ACCESSION   NZ_CP075608
VERSION     NZ_CP075608.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19292310
            Assembly: GCF_018986755.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21607)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     VanA Enterococcus faecalis in Poland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21607)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) Molecular Microbiology, National Medicines
            Institute, Chelmska 30/34, Warsaw 00-725, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/13/2023 03:24:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,539
            CDSs (total)                      :: 3,453
            Genes (coding)                    :: 3,367
            CDSs (with protein)               :: 3,367
            Genes (RNA)                       :: 86
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 39 of 86
            Pseudo Genes (incomplete)         :: 38 of 86
            Pseudo Genes (internal stop)      :: 22 of 86
            Pseudo Genes (multiple problems)  :: 12 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075608.1.
            Bacteria and source DNA available from National Medicines
            Institute, Poland.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21607
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="1207/14"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="p1207_4"
                     /geo_loc_name="Poland: Ostrow Mazowiecka"
                     /lat_lon="52.8071 N 21.8796 E"
                     /collection_date="2014"
                     /collected_by="National Medicines Institute"
     gene            1..933
                     /locus_tag="KKP64_RS17325"
                     /old_locus_tag="KKP64_17335"
     CDS             1..933
                     /locus_tag="KKP64_RS17325"
                     /old_locus_tag="KKP64_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..>1195
                     /locus_tag="KKP64_RS17330"
                     /old_locus_tag="KKP64_17340"
                     /pseudo=""
     CDS             950..>1195
                     /locus_tag="KKP64_RS17330"
                     /old_locus_tag="KKP64_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /name="DUF536 domain-containing protein"
     gene            1273..1953
                     /locus_tag="KKP64_RS17335"
                     /old_locus_tag="KKP64_17345"
     CDS             1273..1953
                     /locus_tag="KKP64_RS17335"
                     /old_locus_tag="KKP64_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            2082..2777
                     /gene="vanR-A"
                     /locus_tag="KKP64_RS17340"
                     /old_locus_tag="KKP64_17350"
     ARG             2083..2774
                     /name="vanR-A"
                     /gene="vanR-A"
                     /gene_name="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2755..3909
                     /locus_tag="KKP64_RS17345"
                     /old_locus_tag="KKP64_17355"
     CDS             2755..3909
                     /locus_tag="KKP64_RS17345"
                     /old_locus_tag="KKP64_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="WP_002305818.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGKL
                     GDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKYFD
                     EINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLA
                     HDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNLQT
                     ITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNILKN
                     AAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGGAG
                     LGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
                     /name="vancomycin resistance histidine kinase VanS-A"
     gene            4124..5092
                     /locus_tag="KKP64_RS17350"
                     /old_locus_tag="KKP64_17360"
     CDS             4124..5092
                     /locus_tag="KKP64_RS17350"
                     /old_locus_tag="KKP64_17360"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="WP_001059542.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKSA
                     PFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAYSP
                     DSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKAVIE
                     RLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQRMKQ
                     GAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQFLLKL
                     QRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
                     /name="D-lactate dehydrogenase VanH-A"
     gene            5085..6116
                     /gene="vanA"
                     /locus_tag="KKP64_RS17355"
                     /old_locus_tag="KKP64_17365"
     ARG             5086..6113
                     /name="vanA"
                     /gene="vanA"
                     /gene_name="D-alanine--(R)-lactate ligase VanA"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6122..6730
                     /locus_tag="KKP64_RS17360"
                     /old_locus_tag="KKP64_17370"
     CDS             6122..6730
                     /locus_tag="KKP64_RS17360"
                     /old_locus_tag="KKP64_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="WP_000402347.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTYE
                     LAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRTEM
                     ISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEAQNR
                     RRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
                     /name="D-Ala-D-Ala dipeptidase VanX-A"
     gene            7136..8068
                     /gene="vanY"
                     /locus_tag="KKP64_RS17365"
                     /old_locus_tag="KKP64_17375"
     CDS             7136..8068
                     /gene="vanY"
                     /locus_tag="KKP64_RS17365"
                     /old_locus_tag="KKP64_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="WP_029656388.1"
                     /translation="MVKEETENEEVVFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQ
                     NPKEHLENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELIN
                     GYGLLDSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYAL
                     PAGYSEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPW
                     HIRYVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPT
                     NLRYEISGNNIDGVIVTVFPGSTHTNSRR"
                     /name="vanY"
     gene            8221..8706
                     /gene="vanZ-A"
                     /locus_tag="KKP64_RS17370"
                     /old_locus_tag="KKP64_17380"
     ARG             8222..8703
                     /name="vanZ-A"
                     /gene="vanZ-A"
                     /gene_name="glycopeptide resistance protein VanZ-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(9056..9343)
                     /locus_tag="KKP64_RS17375"
                     /old_locus_tag="KKP64_17385"
     CDS             complement(9056..9343)
                     /locus_tag="KKP64_RS17375"
                     /old_locus_tag="KKP64_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002333827.1"
                     /translation="MSINTFVYPYPINVFIISRLGMTVEQFCELHGYKQSTVASWVTRN
                     RTVKTLPCDFLYCLSLSSGWSMDDVYRHLLVLEEKFLKSINPKKKKEQVD"
                     /name="hypothetical protein"
     gene            complement(9479..10978)
                     /locus_tag="KKP64_RS17380"
                     /old_locus_tag="KKP64_17390"
     CDS             complement(9479..10978)
                     /locus_tag="KKP64_RS17380"
                     /old_locus_tag="KKP64_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_002333826.1"
                     /translation="MAASLDVVYSTVLQNGIRKFKYKNSHLKPVSFSDQSGKGAIFAYR
                     SKEHMIEGIGLVITSEEGVIENNNRFTHWTPNVFRYGTYADEARMFTKGHSEDNLRQIN
                     TFFVDFDTLDPNFDYGEIILASHEIGFMPTMILRTPHGFQAFYVLDKPAYVTKKSNFKV
                     INVAKMISKNLRNKFSNDYRFPVDVNANHFGITRMPSEENVLFFEPQYRYSFEEWIDWS
                     MREDADKKAEIKNVFVLPSTQEFRQVDEPWFQLLLKSQKIQGEKGVYGRNNVIFTLSLA
                     YFSSGYEVEACELTMLEFNDRLANPLSDRELTRIIRSAYSGKYQAAHREKVVLLCQEWV
                     DANLSEEQLFNKKRGWWKFKKERAVRKYSHSYEWAEDLLSYLNEQSYEEQPYIISTKKE
                     LTERLKIPKRSLDKVLKQLREENKIHFRVRAGRNGGIMLASIHGLMRSVIRLKKESRGA
                     YFAAISEAFGKEYTFIQTALKRLIEPNKIGVQTDLFMIDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            11529..12440
                     /locus_tag="KKP64_RS17385"
                     /old_locus_tag="KKP64_17395"
     CDS             11529..12440
                     /locus_tag="KKP64_RS17385"
                     /old_locus_tag="KKP64_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_002333825.1"
                     /translation="MELSQENLKIVFEYRNVVRRPLTITVANSKGGVGKTTIIRYLSYV
                     LANMGFKVLVVDADPQSNTTKTMLLTKNYHSKDDDIFIIEKTMLAGIVERDLKQLVLPI
                     IENLDCIPSHIDFKNFPKYLTKLYGDAIEGIDADYIEVEKNRISVLKDLLEPVKPEYDF
                     VLIDTPPTMSDFTRNAAYASDYIIMAFQTQSDSLDGVEDYISEEVTPLVEKFDCKTEIV
                     GILPNQLSKGSIDRQVVNDAIEKFGDQNLFDNILPHVKRVQTTPRTGLNTDTYWDKLAY
                     EEVFVPLAKDFIHRIKLFEGEN"
                     /name="AAA family ATPase"
     gene            12442..12813
                     /locus_tag="KKP64_RS17390"
                     /old_locus_tag="KKP64_17400"
     CDS             12442..12813
                     /locus_tag="KKP64_RS17390"
                     /old_locus_tag="KKP64_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002333824.1"
                     /translation="MTDNAKPGFFSKQKPDNSYKEIPKPKSAFTSDNTDLLENIHPEED
                     VLKINNKSLGIPAIVWCEFTALLDTTDNDYTYELLEELIHERTARFDHNQKTTYNRILE
                     RKKEQERERIYKKEKRKKI"
                     /name="hypothetical protein"
     gene            13076..13756
                     /locus_tag="KKP64_RS17395"
                     /old_locus_tag="KKP64_17405"
     CDS             13076..13756
                     /locus_tag="KKP64_RS17395"
                     /old_locus_tag="KKP64_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(14093..14554)
                     /locus_tag="KKP64_RS17400"
                     /old_locus_tag="KKP64_17410"
     CDS             complement(14093..14554)
                     /locus_tag="KKP64_RS17400"
                     /old_locus_tag="KKP64_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            14687..15061
                     /locus_tag="KKP64_RS17405"
                     /old_locus_tag="KKP64_17415"
     CDS             14687..15061
                     /locus_tag="KKP64_RS17405"
                     /old_locus_tag="KKP64_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            15115..17241
                     /locus_tag="KKP64_RS17410"
                     /old_locus_tag="KKP64_17420"
     CDS             15115..17241
                     /locus_tag="KKP64_RS17410"
                     /old_locus_tag="KKP64_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            17238..17858
                     /locus_tag="KKP64_RS17415"
                     /old_locus_tag="KKP64_17425"
     CDS             17238..17858
                     /locus_tag="KKP64_RS17415"
                     /old_locus_tag="KKP64_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            17972..18109
                     /locus_tag="KKP64_RS17420"
                     /old_locus_tag="KKP64_17430"
     CDS             17972..18109
                     /locus_tag="KKP64_RS17420"
                     /old_locus_tag="KKP64_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            18551..18931
                     /gene="mobC"
                     /locus_tag="KKP64_RS17425"
                     /old_locus_tag="KKP64_17435"
     CDS             18551..18931
                     /gene="mobC"
                     /locus_tag="KKP64_RS17425"
                     /old_locus_tag="KKP64_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002319809.1"
                     /translation="MSEQNQNLASNYSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            18913..19827
                     /locus_tag="KKP64_RS17430"
                     /old_locus_tag="KKP64_17440"
     CDS             18913..19827
                     /locus_tag="KKP64_RS17430"
                     /old_locus_tag="KKP64_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            19787..20065
                     /locus_tag="KKP64_RS17910"
     CDS             19787..20065
                     /locus_tag="KKP64_RS17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002351557.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLTQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            20067..20756
                     /locus_tag="KKP64_RS17435"
                     /old_locus_tag="KKP64_17445"
     CDS             20067..20756
                     /locus_tag="KKP64_RS17435"
                     /old_locus_tag="KKP64_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_216788184.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     FIFYLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     ARG             4125..5089
                     /name="vanH-A"
                     /gene="vanH-A"
                     /gene_name="D-lactate dehydrogenase VanH-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2756..3906
                     /name="vanS-A"
                     /gene="vanS-A"
                     /gene_name="vancomycin resistance histidine kinase VanS-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7161..8065
                     /name="vanY-A"
                     /gene="vanY-A"
                     /gene_name="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="98.68"
                     /coverage="99.67"
     ARG             6123..6727
                     /name="vanX-A"
                     /gene="vanX-A"
                     /gene_name="D-Ala-D-Ala dipeptidase VanX-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4125..5092
                     /name="vanH gene in vanA cluster"
                     /gene="vanH gene in vanA cluster"
                     /gene_name="vanH; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2083..2777
                     /name="vanR gene in vanA cluster"
                     /gene="vanR gene in vanA cluster"
                     /gene_name="glycopeptide resistance gene cluster; vanR"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7089..8068
                     /name="vanY gene in vanA cluster"
                     /gene="vanY gene in vanA cluster"
                     /gene_name="vanY; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="98.68"
                     /coverage="107.59"
     ARG             8222..8706
                     /name="vanZ gene in vanA cluster"
                     /gene="vanZ gene in vanA cluster"
                     /gene_name="vanZ; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2756..3910
                     /name="vanS gene in vanA cluster"
                     /gene="vanS gene in vanA cluster"
                     /gene_name="vanS; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="94.79"
     ARG             6123..6730
                     /name="vanX gene in vanA cluster"
                     /gene="vanX gene in vanA cluster"
                     /gene_name="vanX; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     BGC             9687..20061
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9480..10978)
                     /name="rep_cluster_183"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          18914..19824
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg ttttttttgg
     1201 ttctgttgca aagttttaaa tctactatca aataaggtag aataatagaa aaagatagca
     1261 ggaggaatga cgatgaatca ttttaaagga aagcaatttc agcaggatgt gattattgta
     1321 gccgtgggct actatcttcg ttataacctt agctatcgtg aagttcaaga aatcttatat
     1381 gatcgtggca ttaacgtttc tcatacgacg atttatcgtt gggtgcaaga atatggcaaa
     1441 ctactctatc aaatttggaa aaagaaaaat aaaaaatcct tttattcatg gaaaatggat
     1501 gaaacgtaca tcaaaattaa aggaaaatgg cattatttgt atcgagccat cgatgcagat
     1561 ggtttaacct tggatatttg gttacgtaaa aaacgggaca cacaagcagc ctatgctttt
     1621 cttaagcggt tagtgaagca gtttgatgaa ccgaaggttg tagtcacaga taaagccccc
     1681 tctattacaa gtgcctttaa gaaactaaaa gaatacggct tttatcaagg gacagaacat
     1741 cgtaccatta aatacctgaa taatttgatt gaacaagacc atcgtccagt aaagagacgc
     1801 aataaattct atcgaagttt acgcactgcc tctaccacga ttaaaggcat ggaagccatt
     1861 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
     1921 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggattttatt
     1981 ctttatttaa aactttgcaa cagaacctgt aaatgcttat aaattcggcc ctataatctg
     2041 ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta
     2101 ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt
     2161 atacggtttt caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga
     2221 ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa
     2281 aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag
     2341 ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac
     2401 tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa
     2461 aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg
     2521 agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa
     2581 tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg
     2641 gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg
     2701 aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt
     2761 ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat
     2821 atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga
     2881 gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg
     2941 gacgcgatga aattatatca atattccata cggaacaata tagatatctt tatttatgtg
     3001 gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac
     3061 tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag
     3121 ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa
     3181 aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg
     3241 gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag
     3301 gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg
     3361 tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg
     3421 ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa
     3481 ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg
     3541 accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc
     3601 gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg
     3661 gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt
     3721 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
     3781 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
     3841 gataactata cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg
     3901 aggtcctaag agatgtatat aattttttag gaaaatctca aggttatctt tactttttct
     3961 taggaaatta acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc
     4021 gtaagaacga gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc
     4081 ccgttttatt tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat
     4141 tactgtttat ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt
     4201 tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc
     4261 tttcaatcaa tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc
     4321 gctgaagaga gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga
     4381 tacaactgct gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag
     4441 cgttgccgat tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt
     4501 gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga
     4561 catgacagtt ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg
     4621 aggatttgga tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt
     4681 accgtttgat gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac
     4741 ggatacgcac tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct
     4801 tatcaatact gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa
     4861 cgggaaactg ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc
     4921 tgattgcacc caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa
     4981 cgtgataatc acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga
     5041 aaaaaccatt aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag
     5101 ttgcaatact gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag
     5161 agatagccgc taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat
     5221 ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt
     5281 cagctgtact ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat
     5341 atgaaatcaa ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg
     5401 gatccataca aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa
     5461 gctcagcaat ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag
     5521 ctactcccgc cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct
     5581 atcctgtttt tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata
     5641 gcgcggacga attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa
     5701 ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt
     5761 tagttgttgg cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg
     5821 aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag
     5881 cagaggagcg aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta
     5941 gaggtctagc ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag
     6001 tcaatactct gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag
     6061 gtattgcact tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag
     6121 catggaaata ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa
     6181 atatgccact tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat
     6241 tgtagggaca tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca
     6301 agggtacgga ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat
     6361 gcaatgggct gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga
     6421 ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag
     6481 tgccattgat cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg
     6541 atttgatttt atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc
     6601 gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct
     6661 cgaatggtgg cactatgtat taagagacga accatacccc aatagctatt ttgatttccc
     6721 cgttaaataa acttttaacc gttgcacgga caaactatat aagctaactc tttcggcagg
     6781 aaacccgacg tatgtaactg gttcttaggg aatttatata tagtagatag tattgaagat
     6841 gtaaggcaga gcgatattgc ggtcattatc tgcgtgcgct gcggcaagat agcctgataa
     6901 taagactgat cgcatagagg ggtggtattt cacaccgccc attgtcaaca ggcagttcag
     6961 cctcgttaaa ttcagcatgg gtatcactta tgaaaattca tctacattgg tgataatagt
     7021 aaatccagta gggcgaaata attgactgta atttacgggg caaaacggca caatctcaaa
     7081 cgagattgtg ccgtttaagg ggaagattct agaaatattt catacttcca actatatagt
     7141 taaggaggag actgaaaatg aagaagttgt ttttttattg ttattgttat tcttaatata
     7201 cttaggttat gactacgtta atgaagcact gttttctcag gaaaaagtcg aatttcaaaa
     7261 ttatgatcaa aatcccaaag aacatttaga aaatagtggg acttctgaaa atacccaaga
     7321 gaaaacaatt acagaagaac aggtttatca aggaaatctg ctattaatca atagtaaata
     7381 tcctgttcgc caagaaagtg tgaagtcaga tatcgtgaat ttatctaaac atgacgaatt
     7441 aataaatgga tacgggttgc ttgatagtaa tatttatatg tcaaaagaaa tagcacaaaa
     7501 attttcagag atggtcaatg atgctgtaaa gggtggcgtt agtcatttta ttattaatag
     7561 tggctatcga gactttgatg agcaaagtgt gctttaccaa gaaatggggg ctgagtatgc
     7621 cttaccagca ggttatagtg agcataattc aggtttatca ctagatgtag gatcaagctt
     7681 gacgaaaatg gaacgagccc ctgaaggaaa gtggatagaa gaaaatgctt ggaaatacgg
     7741 gttcatttta cgttatccag aggacaaaac agagttaaca ggaattcaat atgaaccatg
     7801 gcatattcgc tatgttggtt taccacatag tgcgattatg aaagaaaaga atttcgttct
     7861 cgaggaatat atggattacc taaaagaaga aaaaaccatt tctgttagtg taaatgggga
     7921 aaaatatgag atcttttatt atcctgttac taaaaatacc accattcatg tgccgactaa
     7981 tcttcgttat gagatatcag gaaacaatat agacggtgta attgtgacag tgtttcccgg
     8041 atcaacacat actaattcaa ggaggtaagg atggcggaat gaaaccaacg aaattaatga
     8101 acagcattat tgtactagca cttttggggt aacgttagct ttttaattta aaacccacgt
     8161 taactaggac attgctatac taatgataca acttaaacaa aagaattaga ggaaattata
     8221 ttgggaaaaa tattatctag aggattgcta gctttatatt tagtgacact aatctggtta
     8281 gtgttattca aattacaata caatatttta tcagtattta attatcatca aagaagtctt
     8341 aacttgactc catttactgc tactgggaat ttcagagaga tgatagataa tgttataatc
     8401 tttattccat ttggcttgct tttgaatgtc aattttaaag aaatcggatt tttacctaag
     8461 tttgcttttg tactggtttt aagtcttact tttgaaataa ttcaatttat cttcgctatt
     8521 ggagcgacag acataacaga tgtaattaca aatactgttg gaggctttct tggactgaaa
     8581 ttatatggtt taagcaataa gcatatgaat caaaaaaaat tagacagagt tattattttt
     8641 gtaggtatac ttttgctcgt attattgctc gtttaccgta cccatttaag aataaattac
     8701 gtgtaagatg tctaaatcaa gcaatctgat ctttcataca cataaagata ttgaatgaat
     8761 tggattagat ggaaaacggg atgtggggaa actcgcccgt aggtgtgaag tgaggggaaa
     8821 accggtgata aagtaaaaag cttacctaac actatagtaa caaagaaagc ccaattatca
     8881 attttagtgc tgaggaattg gtctctttaa taaatttcct taacgttgta aatccgcatt
     8941 ttcctgacgg taccccagta ataaaagata aagtcaaaat cccataataa attttcaaaa
     9001 gaacagacca ttctttgatc ctattaagaa cagataactc ctcctccgtc atcaatcaat
     9061 ccacctgttc tttcttcttt tttggattga tactctttaa gaacttctcc tccaacacca
     9121 ataaatgacg ataaacatca tccatcgacc agcctgaaga aagactaaga caatacaaaa
     9181 aatcacaagg caacgtcttt acggtacggt ttcttgttac ccagcttgct actgtactct
     9241 gcttatagcc atgcaactca caaaactgct ctactgtcat acctaaccgc gaaataataa
     9301 acacattgat cgggtaggga tatacaaagg tattgatact catagtcaga aagacctccc
     9361 agcatattat tacgtttgca ataatagtac tactctttct taagaccagc aagcaaggcg
     9421 gagaaaaaaa ggaacgtatc atagatataa tgtaataggc aatttgcacc ttttaatttt
     9481 aacctgtatc gatcatgaac agatctgttt gcacgccgat cttattgggt tctatcaagc
     9541 gtttcagtgc tgtctggata aacgtgtatt cttttccaaa agcttctgag atcgcagcga
     9601 aataagcccc ccggctttct ttttttagcc ggataacgga gcgcatcaat ccatgtatcg
     9661 aagctagcat aataccccca ttacgaccag cacgaacacg aaaatgaatt ttgttctctt
     9721 ctcttaactg cttcagtact ttgtccaggg aacgttttgg gatctttaaa cgttcagtta
     9781 actctttttt tgttgaaata atgtatggtt gttcttcata ggattgctca ttcaaatagg
     9841 acagtagatc ttctgcccat tcataactat gtgaatactt gcgaacagct ctttctttct
     9901 tgaatttcca ccaaccccgt tttttattga atagctgctc ttcagaaaga ttggcatcga
     9961 cccactcttg gcataatagg acaacttttt ctctgtgcgc tgcttgatat tttccggagt
    10021 aagcggaacg aatgattctg gttaattctc gatcagataa agggttagcc aagcggtcat
    10081 tgaattctaa catcgtcaat tcacatgctt ctacttcgta tcctgacgaa aagtatgcca
    10141 aagacaaggt gaatatgaca ttatttcgac catatacgcc cttttcacct tgtatttttt
    10201 gactttttag caacagctgg aaccagggtt catcgacttg acgaaattct tgagtagatg
    10261 gtaatacaaa aacgttcttg atctccgctt ttttgtccgc atcttctctc attgaccagt
    10321 cgatccattc ttcgaaacta taacgatact gtggttcaaa gaataaaaca ttctcttcag
    10381 aaggcattct tgtgatccca aaatgattgg catttacatc gacaggaaac cgataatcat
    10441 ttgaaaattt attccttaga ttttttgaaa tcattttggc cacattgatc actttaaaat
    10501 ttgatttctt cgtcacatat gcaggcttgt ctagtacata gaaagcttga aatccatgcg
    10561 gtgtacgtag aatcattgtc ggcataaagc cgatctcgtg actggccaag ataatttctc
    10621 catagtcaaa gtttggatcc aatgtgtcaa aatcaacaaa gaatgtattg atttgacgta
    10681 aattgtcctc cgaatgacct ttcgtaaaca ttctggcttc atcagcatac gttccatagc
    10741 gaaatacatt tggtgtccaa tgagtgaaac gattattgtt ttcgatcacc ccttcttctg
    10801 aagtgatcac tagtccaatc ccctcgatca tgtgttcctt tgaacggtac gcaaaaatag
    10861 cccctttccc tgactgatca ctaaaagaga ccggttttag gtggctgttt ttatatttga
    10921 atttacggat accattttgt aataccgtcg aatatacgac atccaaagaa gctgccatga
    10981 ctgttattcc ttcccaaact aaaagagaac acaaataatc ttaaagattt gtgttctccc
    11041 aatagcttag aagtcttaaa tgaatgacaa aacaagcatg tatatattga gttgctcgtc
    11101 aaaaaatggt agaataaatc tgtaagttcg gattgtcagt ccgaaagact tatcaaaatt
    11161 acataagtgg ttgtcagcca cttacataac ttctgagacg agcgagaaca cataggcttt
    11221 tatgtgattc agatagaata gtgaggccaa tttcactttt ttatccgtct ctttttttta
    11281 tgtaattttc ttttaaagtg taaacttttt tttaatgctg taaacctttt tttatactac
    11341 aaagttaccc tatttttaaa catttgtaaa gattatcaag tgacttgatt ataaattata
    11401 ttttataaac tataatttat aaagtataat tgacaacata tttccttctg gtatagtgtt
    11461 cttagacaga tatatatatt atattttgca atttatattt tatattttat aaacgggagg
    11521 tttgtttaat ggaattatcg caagaaaact tgaaaattgt ttttgaatat agaaatgtcg
    11581 tcagaagacc attaactatt actgtagcaa attccaaggg tggagtaggt aaaactacta
    11641 ttataagata cttgagctac gtacttgcta atatgggatt taaagtgttg gtagtggatg
    11701 ccgatccgca atcaaatact acaaagacga tgttgttgac taaaaattat cactctaaag
    11761 acgatgacat tttcattatt gaaaaaacta tgctagctgg gatcgttgaa cgagatttaa
    11821 aacaattagt tcttccaatt attgaaaact tggattgcat tccatcacat atagatttta
    11881 aaaactttcc aaaatattta accaagcttt atggggacgc tatagaagga atagacgctg
    11941 attatatcga ggtcgaaaaa aacagaatct ccgtcttgaa agatttactt gagccagtca
    12001 agccggaata cgattttgtt ctcatcgata ctccacctac tatgtctgat ttcactagga
    12061 atgctgctta cgcttctgac tatattatta tggcttttca aacccaatct gatagcttag
    12121 atggcgttga agattatata tcagaagaag tcactcctct tgttgaaaaa tttgattgta
    12181 agactgaaat agttggaata cttccaaatc agttgagtaa aggtagtata gatcgccaag
    12241 ttgtcaatga tgctatcgaa aagtttggtg atcaaaacct ttttgataat attttaccac
    12301 atgttaagcg agtgcaaaca acaccgagga caggtttaaa tactgacact tattgggaca
    12361 aacttgctta cgaagaagtt tttgtacctt tagcaaaaga tttcattcat agaataaagt
    12421 tattcgaagg agaaaactaa catgaccgat aatgcaaaac caggtttctt ttcaaagcaa
    12481 aaacctgata attcttataa agaaatccct aaaccgaaat cagcatttac ttctgacaac
    12541 acagatttac tagaaaatat tcaccctgaa gaagacgtat tgaagataaa caacaagtcg
    12601 cttggtatac cagcgatagt ctggtgcgaa tttactgctt tgcttgatac tactgataat
    12661 gattatacct atgaactact cgaagaatta attcacgaaa gaactgcacg tttcgatcac
    12721 aatcaaaaaa caacctacaa ccgaatatta gagcgtaaga aagagcagga aagagaacgg
    12781 atatataaga aagaaaaaag gaagaagata taaatcttct tcctttttat attttgtaaa
    12841 gtacaattta taatttatat tttgcaaatt ataaattgca acaaaaatta agcctttaaa
    12901 tacaattaat tactcgctgg caaaggtttt ataaatatta ctttataatt tatattctat
    12961 attttataat ttataaatat aaccagccgt gttctcttga cggttctgtt gcaaagtttt
    13021 aaatctacta tcaaataagg tagaataata gaaaaagata gcaggaggaa tgacgatgaa
    13081 tcattttaaa ggaaagcaat ttcagcagga tgtgattatt gtagccgtgg gctactatct
    13141 tcgttataac cttagctatc gtgaagttca agaaatctta tatgatcgtg gcattaacgt
    13201 ttctcatacg acgatttatc gttgggtgca agaatatggc aaactactct atcaaatttg
    13261 gaaaaagaaa aataaaaaat ccttttattc atggaaaatg gatgaaacgt acatcaaaat
    13321 taaaggaaaa tggcattatt tgtatcgagc catcgatgca gatggtttaa ccttggatat
    13381 ttggttacgt aaaaaacggg acacacaagc agcctatgct tttcttaagc ggttagtgaa
    13441 gcagtttgat gaaccgaagg ttgtagtcac agataaagcc ccctctatta caagtgcctt
    13501 taagaaacta aaagaatacg gcttttatca agggacagaa catcgtacca ttaaatacct
    13561 gaataatttg attgaacaag accatcgtcc agtaaagaga cgcaataaat tctatcgaag
    13621 tttacgcact gcctctacca cgattaaagg catggaagcc attcgaggat tatataagaa
    13681 aacccgaaaa gaaggcactc tcttcgggtt ttcggtctgt actgaaatca aggtattatt
    13741 gggaatccca gcttaaatca tagataccgt aagggatttt attctttatt taaaactttg
    13801 caacagaacc agattatcct agtttagcaa cataaaccat ttattagatg aaaatgagat
    13861 acaaacaaag cgtatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
    13921 aatgcacaaa gaattagata tagataaccg cgaaaaatca agccttaaaa agagcttttt
    13981 acctagacca attaaaaatc acaaagtgag cccctgtatc ggttgataca agggctactt
    14041 ggcatactgt acattatgtc aactatgcaa ttaaataact aactacttca tattagctta
    14101 ataaaataat caaaataact agggggaaaa acattgtata aaaaaatttt tcatttttta
    14161 aataagaata gtaaatataa aaataattta agatatctat tactattaat ccagatagag
    14221 caaaccaagt aaatattcca tgtgtaaagc taaaaaaatt catttgaatc aattcaaata
    14281 aacctaatat agtaacaaat atattaaaaa taatagctgt caataccagt aaattatttt
    14341 ttttattttt tgaattatct tcatttaaaa tagaatttat gttagtacta taaaattcac
    14401 aaattttttt tagtgtctct atatctggtg ttgttttgtt attttcccac tttgaaatag
    14461 tttgacgtgt aacgttaaat tttcttgcca catattcttg agtataccct tttttcactc
    14521 ttaattctct tagcttagat cctaaatcct ccattttttc tcctatgtaa acgattattt
    14581 acataaaatt ttatcataaa aatactaacg cgccaaaaaa gttctttatt ttataaaaca
    14641 cactaaaata aaataattct agtaaaaaag ataggagaaa taagttatga aaaaattttt
    14701 atgtctttct gctttaatgg gtgtcttatt aactagtgga ggaatagcta gtgccacaga
    14761 agctttaaat ctagatgtac ccgaagacca taccgccatt tacggaggag gaatggaagc
    14821 tacagaagaa ggattttctt ctagtaaatt gagatatgct gctggcggcg gggatttcaa
    14881 ctgtggggtt aatggattta aagtttatgc aaattactac catgctagag ctaaacatag
    14941 tgcaacagct aaaaatggac gaggcggtca agtacgatca gttcagaaag ctggagtaag
    15001 agcttatgca acatgcaatg ccacacttac tggaaatact ggttggtgga atgtttatta
    15061 aaaaagagag tttaaactct cttttttatt tacatttgaa aggaaattca aaatatgtta
    15121 aaaaaaataa acgctgtttt atcacttttt gtctgcatta caatactatt tttttctact
    15181 aatgttataa atggtataaa gacttctgaa gcgctatata aaaataccgc tgaaattcat
    15241 atgcaatata aagatgatat ggactttaac agttatataa acgctttgta tgaaatatct
    15301 acacaagaaa atattaatat ttctcaatat agttttactg ctgttaacca actaactatt
    15361 cttgctacaa accctaacgc taacaaacac tggaaatttt ctaaaaaaaa tatattagca
    15421 agaaacgatt ctaaagtcca ttatactaat aacaaaaatt ctaagtacca attaaaactt
    15481 ccaaataatt ttttaaatat aaatatttat cctttttctc atgtggaaaa tattggatta
    15541 agtgaaatat tatatgtaca aggaaactca aacaaattaa ttcctgtact gaaaaaatat
    15601 ggaacaccta ctatttccaa tatcaatcaa tcagatactt ttcaaattaa tactaatatt
    15661 gttcttgtta tctgctatct tagtatattt attgtaatta ctacaattat atttgctttt
    15721 tcaaaaatga aagaaatcac tctaaaaaaa atgttaggct ataactcttt tgatactata
    15781 aaagattcta ttaaagagtt aaacctctta ttttgttctt tgattataac tttcatttta
    15841 tctatttttt tgagcatata ttacagtaac gaaaattttt tcctatcatt tcttctatgt
    15901 agtatttttc attttataat tagtttttta tttatcatag ttactctaag tatctatcat
    15961 ttatctagta taaaaaatta tctaaaaaat tcacgaccac ttaagttaaa tctttgtata
    16021 ttaaatatct gtctattctt aagcatgatc cttctgttaa tagcttctac aaaagtaata
    16081 aatacccata atgaagccga aaataattct cttaaatatt gggaaagaac tactaattta
    16141 tacaaaacaa atattactaa tcaattaaat agaaacaaca ccgtagaaga aaataactac
    16201 ttgaaaaaag ctagtaaatt tgtttataaa atacagaaga attataaaac ttttattatc
    16261 gcgccataca attacgcaac tatccaagag aataataaag aattttttat tggacaaaaa
    16321 gtttatccaa attttgaaga ctatgttagt caacctgctg gtgcaggaat atgtgttgat
    16381 ttaaactatc taaaatatta caatcctatt tcttttgaag aatctactga cttaaattta
    16441 ataaactctg atccactaac tacttacatt ctagtaccta aaaaatataa agaatatgca
    16501 aaaatgattg aaaaaaatta tttagaagac atacagttta gacttgagga ctctcctcct
    16561 caagctcctt ataaaaatcc taatttgaaa aatttaaaaa taaaactaat atttgttaat
    16621 aataaccaaa agtattttag ttttaatacc ttatatggaa aggcaaaaga taactataca
    16681 ataactgatc ccatagttag agttattaat ccagaaataa cagaatcatt attttgggga
    16741 aatatattga ctagtgatgg tggattattt tttgatcaga aacatacttc aaaccaaaac
    16801 ttttttaatc aaattaatcc gtatataaaa gaatttaacc tagaaaatat cataaatttt
    16861 tcttattctg tttttcaaga aactggtgaa ttattatctc aaaaaaaagt aaataaatta
    16921 aatgcatatc tacaatatct tttaatatta ggattattta taataattaa ttttcaaatg
    16981 atttttgtta gttttactat aaattcaaaa aaaatagctt taaaaaaaat atttggatat
    17041 tcaccaataa gtcaagtttt agatttatgt ttactccctt ttacaataga gttatttgct
    17101 actatgtgtt ttcaattgat attttcaaaa aacacttact tgtattttat aatttttatg
    17161 atctttttaa taaatctaat tacttctatt attatttata aaaaaaatag ttttaaatat
    17221 tttaaggaga atattttatg atattatcag ttgagaattt caccctgtca attaataaaa
    17281 aaaagatcaa acaaaattgg aacttttcaa tatcagaaaa agattttatt atagtaacag
    17341 gaaaaagtgg tagtggaaaa actactctgt taaataattt aagcctaata gataaacaat
    17401 ataccggtaa cttattctat tttgaaaaga aagtttctaa acatgatata caacgtctac
    17461 gaagaaatca tattagctat ttatttcaaa actatgctct tcttgaaaat caaacagtac
    17521 aatacaattt cgaattagca aaaaagttta acagtaatat ttccaatgat catatttata
    17581 atttattaac agatttttct ttgccaaaag atatcttaaa agaaaaaatc tttctcttaa
    17641 gcggtggaga acaacaaaga atagcattaa ttagaaatat gttaaaacca tttgatatat
    17701 tatttgcaga tgaacctaca ggaaatttag ataaagaaaa ctcaactttt ataatgaact
    17761 ttctgaaaaa tttaacagaa aaagaaaaaa aagccgttgt actagtaaca catgacttaa
    17821 atgttctaaa ctatggaact aatattatag aattataatt tttacccgtg atgctagctg
    17881 atttaaaact agcaccacgg gtatttattt tatattccat ctctcctatc ccaaaattta
    17941 aataatcact taataaaaag gaggcaccca agtgatttta ttgaaaaaag agtatgaaaa
    18001 attttatgat agacttattt attctttgca attgcccaaa aaattaaacc gtcaattaca
    18061 aaagacttat gaaactaaat caactgaaga atgggagagt tattgctaga tttttggtag
    18121 aagtaacaaa aagtggacat attatcataa tgactcgccg aaagtctgat aaaaaattgg
    18181 ctaaaaatat tgctgaatgt ttaataaaac tatcagtttt agctaaatag ttagtagttt
    18241 acacaaaaaa gcatttatgt tttttgttgc aaagcaacaa tttcatcttc ggtgaaataa
    18301 gcgaaacttt acaaaaagtg gctagcattt gctagctttt tttgtattgt ggagcgtggc
    18361 ttttgagggg gattcaaggg ggagcttgcg cccctttgac aagcggttat ttgcactaaa
    18421 tacgtaggta tttggctagc aaatatagag cttgccaaac ccttttagtg agctttactc
    18481 attgtacctt tttaggtaca atggtattgc gaacgatacg tccgtaggac gtactgaaag
    18541 ggtgggactt atgagcgaac aaaatcaaaa tttggctagc aattattcta aaaaatatac
    18601 ctatcgatcc gagccaaaac aaatcagttt tcgagtgagc gaatccgaat ttgcaaagct
    18661 gaagcaatcg gctgaagctt tgcaaatgag tgtgccggct ttcgtgaaag ccaaggcaca
    18721 aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt gcccaggcaa tcgcgcgcga
    18781 tttagccaaa gctggaggga atatcaatca aattgctaag tggtgcaata cacaccaaca
    18841 tgacgttgct tctggtgacg ctcaacgttt atccgaaaac ctaaaaatca tgcaaaagga
    18901 gctacaaaag atatggcaac aattaaggta agccgaacca cttcttgtag tcgtgcaatt
    18961 aattacgcag aaccacgagc aaccattaaa acaggaataa actgtgacgt cgattatgcc
    19021 aaaagtgaaa tgaaacaaat tcgcatgctg tatggaaaag acgaccgtgt acaggcacac
    19081 ttattgattc aatcttttcg accaggagaa attaccgctg aaaaagctaa tcaactaggg
    19141 gcagagtatg caaaaaaaat agcccccgat caccaaatag ctatttacac acatacagac
    19201 aaagaccata ttcataatca cattgtcatt aactccgtta atctggagac tggaaaaaaa
    19261 tttcaagctc atggtcaagc atttctaaac aaatgttatg acctaaatga cgagatttgt
    19321 cttgcacata acttaccaat cactgagcga ggaaagaaac cagaaaaacg aaccatgtct
    19381 gaaataaaat taaaagaaaa aaatgaacct gtttggaaag acgaaattcg gattgcgatc
    19441 gatcaaacaa tgaaaaacaa aaaaaccaga acctatgacc aattttgtaa ttccctcaaa
    19501 acattgggga ttcattgctt caatcgcggg aaaaatttca cttacgaatt gatccacaaa
    19561 aagaaaaaag tacgatccaa cagactagga aaggactacg aaaaggagac gattttacat
    19621 gagctggaca gacgagaacg aacctataac caacagcgaa ccaacgagct acgaagagct
    19681 cgagctagca ttcgcgaaac tgcagaagaa gtacgaagag aacaacgaac tcttacgcct
    19741 gaacaaccgg aaattagacc ggctcgaaga aaccatgaac aacacgttga aagaggaaca
    19801 gaccaccaac aagaattatc gctctaaaac gttaaaagac atagaaaacg aaataaagaa
    19861 acaacgtgct tttctcgttc cttatacgca agaaaggata caaactacga ttgatttaca
    19921 agttaaaaaa ctccatcaag agctattaga ccagcgaaat aaggaacaga aaaaggtgct
    19981 tactcaacga tcattggatt ttcttgcccc cttatgttgc ttagtcctga cactgttcgc
    20041 attttggtta gtatggggag gctaaaatgg cattttattt accctatcta ctcatttttg
    20101 ttagtatttc aggaagtatt tggctcatct ataaaatttt tcaaacaaga tattcattaa
    20161 aaggctctaa aattcgattt aagcgatttt tctttcttgg ctttatattt tacttgataa
    20221 tcgtttcaag cggcttattc ggcgttttag agggaaataa gcgcgtttct agaagtattt
    20281 tattaggaaa tgtcactcaa aagtatgaat cagcgcgtga taaaaagaaa aaagagcaag
    20341 tattagcaca aaaaatagaa aaatttaccg cttgttatga ggacatgaat gatatttttg
    20401 tgaagcaaga aaagcgtttg acggataaaa atatggaaac actcactcga ctttatcgga
    20461 atttgccaga agaacagcaa aaagagtacc aagaaaaata tgaacaagta aaaaaagacg
    20521 tgcaatatgt gaaagatacg aaagtggaag aagcttgtag tgatttattt ggagatacaa
    20581 ttcctttttc aactagcgaa caagaacgaa aagaacgaca acaatcagtc acatatgaac
    20641 gctatgaaat cttgcttcaa caagcaatta acatacaaaa tctgactaaa aaagaaacag
    20701 ctttaaatta tttaaaaagc gtcaaggaat ggttagatca gcaacagcaa aattgaattt
    20761 gattgaaatt catgttaaac taaacatgca aatgttcaaa cattagatac acgaaagccc
    20821 cagagaggtc ggattctctg gggctttttt taggttgatg tcgcctttac tgattttact
    20881 tcttgtttcg gttattcaac caataactga atacagagat acaaactcct acaagaaggg
    20941 ggcaaataaa cagttcaaac atcagataca cctccttcca gtcgcaagac tgtcggatag
    21001 gcgacaagtt attataccct acaaaattaa atctgtgtgg caattttaag tatctcttat
    21061 ttccgggttg ttagggcttg ccctgaccgc ctgtagggcg ctttaaattc cagataaagg
    21121 tttttatact aagactgtct taaaacggct tagacaagct cttagagcct caaccctcct
    21181 ttttgaacaa tggttttagc aaaggagctg gaggcgactg cgcaggtata atgtgagagc
    21241 gtagcgatca cacctttttg gcgaccgtag ggagcgaggt tttgaattag cagtttagaa
    21301 aagttcgcga acctgtttaa agaaaaaata attgtttttt taggtgggaa attttgtctc
    21361 cctcttttat aatctttttt taaacctctt tttaaacctc tttaccgtcg gctgtgtcct
    21421 tactccccca agggatacaa aattttatag gcatgaaatt atctgtttat aggcatgaaa
    21481 ttatctgttt ataggcatga aattatctgt ttataggcat gaaattatct gttttaggta
    21541 ttgtttttag acataatatg tcctaaaatg atgtcaataa aatcatttag actggagtaa
    21601 caactaa
//
