LOCUS       NZ_CP075558             2317 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain RGF99-1 plasmid pRGF99-1-2k, complete
            sequence.
ACCESSION   NZ_CP075558
VERSION     NZ_CP075558.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18963532
            Assembly: GCF_018502585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Plasmids shapes the current prevalence of tmexCD1-toprJ1 among
            Klebsiella pneumoniae in food production chain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2021) College of Veterinary Medicine, Yangzhou
            University, No. 567, Wenchang Middle Road, Hanjiang District, Yangz,
            Yangzhou, Jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:16:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,730
            CDSs (total)                      :: 5,606
            Genes (coding)                    :: 5,448
            CDSs (with protein)               :: 5,448
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 71 of 158
            Pseudo Genes (incomplete)         :: 92 of 158
            Pseudo Genes (internal stop)      :: 41 of 158
            Pseudo Genes (multiple problems)  :: 35 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075558.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RGF99-1"
                     /isolation_source="a feces sample of swine origin"
                     /db_xref="taxon:573"
                     /plasmid="pRGF99-1-2k"
                     /geo_loc_name="China: Nantong, jiangsu province"
                     /lat_lon="32.39 N 120.56 E"
                     /collection_date="2019-05"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            join(2128..2317,1..347)
                     /locus_tag="KI681_RS26840"
                     /old_locus_tag="KI681_26845"
     CDS             join(2128..2317,1..347)
                     /locus_tag="KI681_RS26840"
                     /old_locus_tag="KI681_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150440.1"
                     /translation="MKKVYTAIILIVLLCGGVLSANYIFLQRHMNEVLKEDPRNDGISV
                     WVYYKWFVNSSEINYDLRSVSAENSSLDVSRVMLQFAEKVKDYDFSKVYLSYRGKDKFY
                     LKGEYFKTLGQEYGIQNPVYTLRTIPENVYMLNGERAYSVWEGGLLGVMGKQMEDLSDF
                     SKAWYLDDFIKSMSD"
                     /name="hypothetical protein"
     gene            complement(728..841)
                     /locus_tag="KI681_RS28900"
                     /old_locus_tag="KI681_26850"
     CDS             complement(728..841)
                     /locus_tag="KI681_RS28900"
                     /old_locus_tag="KI681_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            1603..1839
                     /locus_tag="KI681_RS26850"
                     /old_locus_tag="KI681_26855"
     CDS             1603..1839
                     /locus_tag="KI681_RS26850"
                     /old_locus_tag="KI681_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947370.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          1195..1370
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.358"
                     /coverage="82.0"
                     /evalue="3.53e-41"
     Typing          complement(729..1212)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.418"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 ctgtttctgc agaaaatagt tcactggatg ttagtagggt gatgctgcaa tttgctgaaa
       61 aagtgaaaga ttatgatttt agtaaggttt atcttagtta cagagggaaa gataagtttt
      121 acttaaaagg agagtatttt aaaactcttg ggcaggaata tggtattcaa aaccccgtat
      181 atacattaag aacaattccg gaaaacgtgt atatgttaaa tggagagaga gcttatagtg
      241 tttgggaggg tgggcttctg ggtgtaatgg gaaaacagat ggaagatctt agtgatttct
      301 ctaaggcttg gtacttagat gatttcatta aaagcatgag cgattaattt gaagcgatca
      361 gtatacattg acgattagct cacgcaaatc gctcattggt cactggggtg tttttactcg
      421 tagatgcttt agatgtgtga cctgctcccc gtggattaca aggccgtcat ctgataatgc
      481 ccctaatggg tcagatctct aggatagtag caatacagca aggaacttct tgagatcctt
      541 tctgttgcgt cgtaatctct tgctctgtaa acgaaaaaac cgcccagcaa ggcggttttc
      601 tcgaaggttc tcagagcagc aactctttga accgaggtaa ctggctagtt agaggagcgc
      661 agtcaccaaa actgttcgtt tagtttagcc ttaactggcg catgacttca agactatctc
      721 ctctaaatca gttaccagtg gctgctgcca gtggcgcttt tgcatgtctt accgggttgg
      781 actcaagacg atagttaccg gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca
      841 tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagat
      901 aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc
      961 acgagggagc caccaggggg aaacgcctgg gatctttaag tcctgtcggg tttcgccgcc
     1021 actgatttga gcgtcagatt ttgtgatgct tgtcaggggg gcggagccta tggaaaaacg
     1081 gcagcgccgc atcctttttc gggcccttct tgacctcagc ttcatttcgt tacctcgtca
     1141 ttcgtgagca ggtaacgccc gccgcagtct cacggcagga gcgtagcgac cgagtgagcg
     1201 aggaggcgtc atttcaccat catcagcatt ttgcacttac gcaccgcagc cgcatttttc
     1261 ccctgccaca tgaagcactt ccctgagcca ccacgcccaa ctaacatatt aagccagtat
     1321 acactccgct agcgctacgt gactggtccg gcgctgcgcc cggaacccgc aaattcagaa
     1381 actgaaagcg ggacaagatg tgtttttgga gtaccgccat catgatgccg gcaccaaaac
     1441 gatatctcag ggctgcgccc cgataccccg ccaacgcttc acggcaccgg attaaaatcc
     1501 gttcccgttc accgtttctc agggccgttc ccggctgcag gttcttcatg aggttcgccg
     1561 tgtctggcag actgtgcaga gttattctga ccggagaacg ccatgaaacg actgataaac
     1621 ctgacgcctg ccgagaaaag atttctcgac gatgctgttg ccgccgctga gagagcgtca
     1681 ggcaagaagc tgaatcagcc caacaggcac attgtcctta acagggctcg tgcacagatc
     1741 gaatcgcagc gccaggcaga acggcagcga tctgcgcgtg aggaggagcg gcagcaggcg
     1801 gaatttacct ggtcgcgtcc gcgggcgccg cgtcgctgat tgccactttc gtcggttttg
     1861 tggggagttc agggcgaaac agcgtggtat tcactccggt gcatagtcat tcgccaccgt
     1921 aaaactcacc agagcgattc cgggcggttt tctgggggta agacgtgctc ttttgtggct
     1981 gaaggcgcag aaacgccctg aggccgtttt cgcggtgcgc gtaagaagac gttatggtaa
     2041 atcgatgctt aattccttgc taacttatgg caccttagct taaaaatctc agggtaaact
     2101 ttagagcagt tgagatggat tatagaaatg aaaaaagtat atacagcaat catattaata
     2161 gtactcttat gtggtggtgt tttatctgcc aactacattt ttcttcaaag gcacatgaat
     2221 gaggttctga aagaagatcc aaggaatgat ggtattagcg tttgggttta ttataagtgg
     2281 tttgtaaact cttccgagat taattatgac cttcgtt
//
