LOCUS       NZ_CP075557             6115 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain RGF99-1 plasmid pRGF99-1-6115bp,
            complete sequence.
ACCESSION   NZ_CP075557
VERSION     NZ_CP075557.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18963532
            Assembly: GCF_018502585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Plasmids shapes the current prevalence of tmexCD1-toprJ1 among
            Klebsiella pneumoniae in food production chain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2021) College of Veterinary Medicine, Yangzhou
            University, No. 567, Wenchang Middle Road, Hanjiang District, Yangz,
            Yangzhou, Jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:16:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,730
            CDSs (total)                      :: 5,606
            Genes (coding)                    :: 5,448
            CDSs (with protein)               :: 5,448
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 71 of 158
            Pseudo Genes (incomplete)         :: 92 of 158
            Pseudo Genes (internal stop)      :: 41 of 158
            Pseudo Genes (multiple problems)  :: 35 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RGF99-1"
                     /isolation_source="a feces sample of swine origin"
                     /db_xref="taxon:573"
                     /plasmid="pRGF99-1-6115bp"
                     /geo_loc_name="China: Nantong, jiangsu province"
                     /lat_lon="32.39 N 120.56 E"
                     /collection_date="2019-05"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            join(6100..6115,1..1112)
                     /locus_tag="KI681_RS26790"
                     /old_locus_tag="KI681_26795"
     CDS             join(6100..6115,1..1112)
                     /locus_tag="KI681_RS26790"
                     /old_locus_tag="KI681_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_122008136.1"
                     /translation="MKAVAIDGSVGSVSNEDVLIDTLSEQEQSAIFLFSMRGNWDFIRD
                     FLKHHQLEKMMSNSRRSTYEKLSISFNELKNKNKAAFDLVFKEIKDYALSILTISNKAI
                     YSLNLPKKELGTLLKNISSEPQDNALDIVDRTEDLSSKKNNSCWIDYENCKVCFFPTIK
                     EANKKTSLDPKKHAGMEGFDSLYGFELVRNQYIDCLLLTKDKAFMLVDVIDDDSHEYCK
                     QSYDLLLPKIKTEIIKHTHVANISTKDIFNSVGLLCVQDTDPYKKANYSVHEINFITPE
                     LSRYTAKKSPNKPDIRKDTFNKDGLKNNIQSLIFYKVSFNIDRSHPVIGFKYKVIVSIP
                     GSYSRAAHGKGKVDYFLSTNFLNMSDFNIIEPLIL"
                     /name="hypothetical protein"
     gene            1132..1557
                     /locus_tag="KI681_RS26795"
                     /old_locus_tag="KI681_26800"
     CDS             1132..1557
                     /locus_tag="KI681_RS26795"
                     /old_locus_tag="KI681_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227343605.1"
                     /translation="MATLKERISSLVIYDEIESDTIKFARAAMCLNENPKSVVLSMGSI
                     KKIIENNNIKASHIDLIALLTHPDIDFLQLEYVLLVDDYDDPIEISNDAMADAYKNGYL
                     QNPKNPDHIIYDYDDYVFPTFIITDSYVEQSNRRHEC"
                     /name="hypothetical protein"
     gene            1551..2186
                     /locus_tag="KI681_RS26800"
                     /old_locus_tag="KI681_26805"
     CDS             1551..2186
                     /locus_tag="KI681_RS26800"
                     /old_locus_tag="KI681_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227343604.1"
                     /translation="MLNFQFDNLSDSSPDELKLFQNITRFRKAREGWDDFEKLIHELLI
                     DASTSMSESRNHLKYNDTSEDGYSAVLFYILSGYKNYGLKVNREANNGGHCDIAIELGK
                     HKWLGEAKKAKDYQWVYDGFLQLTTRYSVSGANSKSGGLILYCNQKKVNNFMSNWMMEL
                     KSRFPQISKEECYDDDHNIKITTHSHGVSGNTYKVIHIPIYLNYSPEK"
                     /name="hypothetical protein"
     gene            complement(2164..3093)
                     /locus_tag="KI681_RS26805"
                     /old_locus_tag="KI681_26810"
     CDS             complement(2164..3093)
                     /locus_tag="KI681_RS26805"
                     /old_locus_tag="KI681_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_131368061.1"
                     /translation="MNNAVLELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWRIAVWQPELYTLDWLADSLDLSAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERASAYNLQFAAPLD
                     ENEVNGIAKSIAKWTNNKFNPETFSEYVKRTHNSGTQKKRGALGGKASRGGGRKKNPNS
                     VESLEPWKEQGISRRTYYLRKAISQENS"
                     /name="replication initiation protein"
     gene            complement(3286..3570)
                     /gene="nadS"
                     /locus_tag="KI681_RS26810"
                     /old_locus_tag="KI681_26815"
     CDS             complement(3286..3570)
                     /gene="nadS"
                     /locus_tag="KI681_RS26810"
                     /old_locus_tag="KI681_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            complement(3572..3904)
                     /locus_tag="KI681_RS26815"
                     /old_locus_tag="KI681_26820"
     CDS             complement(3572..3904)
                     /locus_tag="KI681_RS26815"
                     /old_locus_tag="KI681_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000017531.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSVRVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKTLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4060..4305)
                     /locus_tag="KI681_RS26820"
                     /old_locus_tag="KI681_26825"
     CDS             complement(4060..4305)
                     /locus_tag="KI681_RS26820"
                     /old_locus_tag="KI681_26825"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_306293335.1"
                     /translation="MTKTMTEMEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATS
                     QALTHSDRVCQHLSSQVESLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4354..4839)
                     /locus_tag="KI681_RS26825"
                     /old_locus_tag="KI681_26830"
     CDS             complement(4354..4839)
                     /locus_tag="KI681_RS26825"
                     /old_locus_tag="KI681_26830"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_201156124.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMLRASVSTAMAWIFICFALLIGILSGIIWFQGTIITSRLSEMD
                     SYSQQLESLKEKSGAGVAIYSDDKHRNTYMVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<4886..5308)
                     /locus_tag="KI681_RS26830"
                     /old_locus_tag="KI681_26835"
                     /pseudo=""
     CDS             complement(<4886..5308)
                     /locus_tag="KI681_RS26830"
                     /old_locus_tag="KI681_26835"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5517..5843)
                     /locus_tag="KI681_RS26835"
                     /old_locus_tag="KI681_26840"
     CDS             complement(5517..5843)
                     /locus_tag="KI681_RS26835"
                     /old_locus_tag="KI681_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_122008135.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPDIAPELLRQVSGIGNNLNQIARRLNQTDSLTPAERVSLLVVLTSLDRQLGELLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          5518..5690
                     /name="Col(Ye4449)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.143"
                     /coverage="90.0"
                     /evalue="1.12e-30"
     Typing          complement(3969..5527)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.084"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgatgggag tgtaggaagc gtatctaacg aggatgtgct tattgatacc ctaagtgagc
       61 aagaacagag cgccatattt ctgttttcga tgagaggaaa ctgggatttt atccgcgatt
      121 ttctaaagca tcatcagtta gaaaaaatga tgagcaatag ccgccgctca acttatgaga
      181 agctgagtat tagctttaac gagctaaaaa acaaaaacaa agcagcattt gatctagttt
      241 tcaaagaaat aaaagactat gcactttcaa tactgaccat cagcaacaag gcgatttaca
      301 gtcttaattt gccaaaaaag gaactgggca ctctgctgaa aaacatatca tcagaacctc
      361 aagacaacgc attagatatt gtagatagga ctgaagacct cagttcgaaa aaaaacaact
      421 cttgctggat agattatgaa aactgcaagg tatgtttttt tccaacaata aaagaagcca
      481 ataaaaagac atcgttagat ccaaaaaagc acgctggaat ggaagggttc gattctcttt
      541 atggatttga attagtaagg aatcagtata ttgattgtct tttgttaaca aaagataaag
      601 cttttatgct tgttgatgtc atagacgacg atagccacga atactgcaaa cagtcgtatg
      661 acttattgct accaaaaata aaaacagaaa taattaagca tactcatgta gcgaatatct
      721 ctacaaaaga tattttcaat tctgttgggt tactttgcgt ccaagacact gacccatata
      781 aaaaagcgaa ctactcagtt cacgaaatta acttcatcac tcctgaacta tctaggtaca
      841 ctgcaaaaaa atcgcctaac aagcctgaca taagaaaaga cacatttaat aaagatgggc
      901 taaaaaacaa tatccaatca ttaatattct ataaggtttc atttaacatt gacaggtcac
      961 atccggttat aggtttcaaa tataaagtga tagtaagcat accaggaagt tattcacgag
     1021 cagctcacgg aaaagggaag gttgattatt tcttatcaac taactttctt aatatgagtg
     1081 atttcaatat tatagaacct ttgattcttt aacatgaata tagaaactgc tatggctacg
     1141 ttaaaagaaa gaatctcatc actagtcatt tacgatgaga tagaaagcga tactataaag
     1201 ttcgctaggg ctgccatgtg cttgaacgaa aaccctaaaa gtgttgtttt atcgatgggg
     1261 agcataaaaa agataataga aaacaacaac ataaaggcaa gtcatatcga tttaatcgct
     1321 ctactaactc atcccgacat cgatttctta cagcttgagt atgttttact cgtggatgat
     1381 tacgatgacc ctattgagat aagcaatgat gctatggctg atgcttataa aaatggttat
     1441 ttacaaaacc ccaaaaaccc agatcatata atatacgatt atgatgatta tgttttccca
     1501 acttttatta tcactgactc gtatgtagaa caaagtaacc ggcggcatga atgctaaact
     1561 ttcaattcga taatttatca gattcatcgc ctgacgagtt gaaacttttt caaaatataa
     1621 ctcgctttag gaaagccaga gaaggctggg atgatttcga gaaactgata catgagttac
     1681 tcatagacgc atcaacatcg atgtcagaat ctaggaatca cttaaagtac aatgacacgt
     1741 cggaagatgg ttattcagct gttctttttt atattttaag tggatacaag aattacgggt
     1801 taaaggtaaa cagagaggct aacaatggtg gtcattgtga tatcgctata gaattgggaa
     1861 aacacaaatg gcttggagaa gcaaaaaaag caaaagatta tcagtgggtt tatgatggtt
     1921 ttttgcagct aacaactaga tattcagtta gcggtgcaaa ctcaaaatca ggcgggctga
     1981 ttttgtactg caaccagaag aaagttaaca acttcatgtc gaattggatg atggaactaa
     2041 agtctagatt cccacaaatt tcgaaagaag aatgttatga tgatgaccat aacataaaaa
     2101 taaccactca ttcgcatggc gtgagtggga acacgtacaa ggtcattcac atccccatat
     2161 accttaacta ttctcctgag aaatagcttt tctgaggtaa tacgttctcc tgcttatccc
     2221 ttgttctttc cagggttcaa gggattctac tgagttggga tttttcttcc gtcctcctcc
     2281 tctacttgcc ttaccaccaa gagccccccg ttttttttga gttcctgaat tatgggttct
     2341 ttttacatat tcagaaaatg tctcaggatt aaatttatta ttagtccatt tagcgatgct
     2401 tttagcaata ccattaacct cattttcatc cagaggtgct gcaaattgca gattgtaggc
     2461 actggctcgc tcatagcagg cttgcagcca ctgctcatac tcgggccatc cctgacggat
     2521 agcgcggtat gcccacttgc gggttttgtc gaacagggtg cagtttcgac ctaatccata
     2581 gtcggcgacg atctctttgt cgttcgccgc gctcaggtcg agtgagtcag caagccagtc
     2641 cagcgtgtag agctccggct gccacacggc aattctccag tggccatggt tcggattttt
     2701 gcagattagc cccgaatacc ccgcgtcagc ctctaatttt ttacgcagcg cgttctcaac
     2761 ggcggcagcg tagcgtagcg gcttcatttt tccgtctggc gcagtgcgaa tcgatgtttt
     2821 gagggcgtac agcagatgcg cgtgtccgtt gtcggggtta gtgatggtca gtgtaggtgc
     2881 cggtgcgtta cggtcgctcc agtcgatggc ggcaccggcg cgatcgacgt cgaatcccag
     2941 ccagaacatg gcgtgaggct gattgaactg gatgtatttc gccagaatgg cgcgttcctt
     3001 accggcaatg cgtacaccaa agtgtaaatc atccgagaag tacggcttgt gcggtaagcg
     3061 gtcgttgaaa agttcgagta ctgcgttatt caattcgccc tgcgcttatg gcgggctgtt
     3121 gtctggcacg tttcatgtgc taatatcctt tcaaggtttc gacctggccc tggttatcat
     3181 gtgcatccgc caagattgag catgatttcc ggggctttgt tttttctgtt gccgttaaat
     3241 tacacgcgaa cggcctcgcc tgtcgaatcc ttctgtcaca acggcttagt gcgccgccag
     3301 ttcctggaag aatttcgggt tcgcctggat ggttgccagt accttcgccg ccagtccggt
     3361 cgggttacgg cgtccggttt cccagctctt gatggtgtcg aggctggttc ccagcacttt
     3421 tgccatctcg ctctgagata cgttcagctg tgccctgatg gctttcacgt ctgccagctc
     3481 gtaacgggtg acgcgggcag gctctctgac gccgtttttg atttcaacgg cttcttccag
     3541 cgatgctttc agctcgtcaa aaaaactcat gtcacacctc gtctttcagt tgttgggtca
     3601 gcgtcttcag cgcggctttt tccgcgtccg tcaggctgtc tttggtgctt ttcgggtagg
     3661 ccatcaccag atagatcacc tcggcggtgg ccagaaagta aatcaccctc acgctgccgc
     3721 tttttccctg gttgcccgtc gccattctga ttttgcgcag cccaccggtt ttctggatga
     3781 gatcgccttt gtccggcgac tcaatcagcg tcttttgcag ctccctgagt tcttcatccg
     3841 tggcgatctg ttttatctga cgggtaaaca tcggggtctc gataaactca atcgcgtgac
     3901 tcacggctgt ctcctgtgtt caatggcgta catagtacac ttaattttaa tttcagaaaa
     3961 gcggtcacat cgaggggcca taatagcggg tcggcttttt ctgcggctct ggctgctgct
     4021 cacgcagctt ctcggcgtag gcgcggtcgt gctgtctaat cagcgcatca agcggctgac
     4081 gttcctgctc aagctctcga cctgcgcgcg caagctctct acctgactgc tcaagtgctg
     4141 acacacccgg tcgctgtgcg tcagcgcctg tgaagtagcc tcgaacatgt gctgcaagct
     4201 gctgtagctg tcctgccacg cctgctgctg ttgctcgtag cttgtctgta agctctctaa
     4261 ggcgctcaga agctgttttt ccatctcggt catggttttg gtcatcctcg gtgttaaaaa
     4321 cgtgctgtgt cgtttcaggg cgctctgacg gggttatttc gccgtgtatt tcactacggt
     4381 gttgtcggtc gtcgatgtat aggtttccac gccgtgcgcc tgcttcggca gcaccaccat
     4441 gtaggtgttc cggtgcttgt cgtcgctgta gatcgccacg cccgcgccgc ttttctcttt
     4501 caggctctcc agctgttggc tgtagctgtc catctcgctc aggcgcgagg tgatgatcgt
     4561 gccctgaaac cagattatcc cgctcaggat gccgatcagc agggcgaagc agatgaatat
     4621 ccacgccatc gccgttgaga cagaggctct gagcatgccc gtcagtctgc cgttctgctc
     4681 gcggatagcg ttgctgatgc tctgcgagct tgattgcagt tccgcgctga tagacgtctc
     4741 cagcctcgcg aactcgcgct tcacgctctg ctcggtatcc tgcgcctgct gcttcgattt
     4801 ctcctcgaag cccttcgcca aatttaaaat ctcgctcata aatcattcct ttcagccgga
     4861 tatttcggcc gccgtccgga tcggcaatgc tgatgctgct tttcgtttcc cgcgccacgg
     4921 tcaggccata gctttccagc tgcgtcacca cgtcctgccg gctgcggatc acgccctgct
     4981 gcatcaggtt catcagcccg gcggtaattt tctctgccgc ctgctgtttg tcgcggggca
     5041 gatcgctggc ctgcgtcagc gggcggcgat aagtcgggtc gttcgggtca cgcagcccca
     5101 gccggtcgtt ggtcagggtc tgccaggcgt tgacgcgggg gcggtcggcg cggtcaaagt
     5161 agggttgcag gcgttttccg ctctgcaatt cgatgttcgg gatgacaaaa ttcagctcaa
     5221 gccgtccttt gtcacggtgc tccacccaca ggcaggcgta ctggtcgcgg tcaaggccag
     5281 tcagcagcgt gtgctcccat tcatccatca ggcgcgattt ctccgcgtct gcaatgtccg
     5341 gctcctggaa tgacagcacg cctgaggtgt aggcgcgggc aaagtcgcag ccgtcgatca
     5401 gctcgcgcac atgttcaggg ttgccgcgca gtacgctggc ctgctcgcgc tgccggtctt
     5461 ttcccagcag ataatccacc ggcccgctgc cggaacccgc tccccgggag tgaattttaa
     5521 cgatcacggt tctgctccag cagctcaccg agctgacggt caaggctggt cagtacgacg
     5581 agcagggaga cgcgttcagc aggcgtcagg gaatcagtct ggttcaggcg gcgagctatc
     5641 tggttcaggt tgttgccaat accgctgacc tgtcgcagca gttcgggcgc aatgtcaggt
     5701 acacgacgct tgcgtgaagt gtgttcaccc agaccaagct gacgaagcca ctctgcaagg
     5761 tgagtccggt cacagcgatc aagcagtcgc tgatgctcag tttcggtgag ccggattttg
     5821 atctctttcg ttcgcttttc cataggctcc gctgagagaa aattcgcacc accgtgagaa
     5881 tttttcgcag tggatgccag gggtttcggg gggcggcgag ccccctgaac cagtcacggt
     5941 ggcacctcgt tagaggggac gctgtgtgta ctggcttaag ggagtaaatc atagtgtggg
     6001 agtaagtgat aggaaaacaa aacaaaccct agaacagggc tgttatctga tataggcttg
     6061 tttcagtgca atttgataaa caacaaggca tattcaaaga tgaaagctgt ggcta
//
