LOCUS       NZ_CP075556             6598 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain RGF99-1 plasmid pRGF99-1-6k, complete
            sequence.
ACCESSION   NZ_CP075556
VERSION     NZ_CP075556.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18963532
            Assembly: GCF_018502585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Plasmids shapes the current prevalence of tmexCD1-toprJ1 among
            Klebsiella pneumoniae in food production chain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2021) College of Veterinary Medicine, Yangzhou
            University, No. 567, Wenchang Middle Road, Hanjiang District, Yangz,
            Yangzhou, Jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:16:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,730
            CDSs (total)                      :: 5,606
            Genes (coding)                    :: 5,448
            CDSs (with protein)               :: 5,448
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 71 of 158
            Pseudo Genes (incomplete)         :: 92 of 158
            Pseudo Genes (internal stop)      :: 41 of 158
            Pseudo Genes (multiple problems)  :: 35 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075556.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RGF99-1"
                     /isolation_source="a feces sample of swine origin"
                     /db_xref="taxon:573"
                     /plasmid="pRGF99-1-6k"
                     /geo_loc_name="China: Nantong, jiangsu province"
                     /lat_lon="32.39 N 120.56 E"
                     /collection_date="2019-05"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            130..549
                     /locus_tag="KI681_RS26740"
                     /old_locus_tag="KI681_26745"
     CDS             130..549
                     /locus_tag="KI681_RS26740"
                     /old_locus_tag="KI681_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_094307662.1"
                     /translation="MAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRREFETSE
                     LIRAYGELKRNETPERHTAGQAEKPHNQQTERILREVNELKQCLTLMLEDKQAQDTERR
                     RQEAEREQLQNEIVQLRQALEQEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            630..1058
                     /locus_tag="KI681_RS26745"
                     /old_locus_tag="KI681_26750"
     CDS             630..1058
                     /locus_tag="KI681_RS26745"
                     /old_locus_tag="KI681_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_094307660.1"
                     /translation="MKRSGGTRYMYLSALITVTAALTACTPKTSLEQHTRHYVYASDDG
                     FDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAEKAQQRIKEFHSEEFLQSLRTT
                     TNFAGRKYTNSDTPSPEKMKLLADTISAIYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1283..1492
                     /locus_tag="KI681_RS26750"
                     /old_locus_tag="KI681_26755"
     CDS             1283..1492
                     /locus_tag="KI681_RS26750"
                     /old_locus_tag="KI681_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            complement(1910..2143)
                     /locus_tag="KI681_RS26755"
                     /old_locus_tag="KI681_26760"
     CDS             complement(1910..2143)
                     /locus_tag="KI681_RS26755"
                     /old_locus_tag="KI681_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2150..2668)
                     /locus_tag="KI681_RS26760"
                     /old_locus_tag="KI681_26765"
     CDS             complement(2150..2668)
                     /locus_tag="KI681_RS26760"
                     /old_locus_tag="KI681_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2706..3404)
                     /locus_tag="KI681_RS28895"
                     /old_locus_tag="KI681_26770"
                     /pseudo=""
     CDS             complement(<2706..3404)
                     /locus_tag="KI681_RS28895"
                     /old_locus_tag="KI681_26770"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3346..3669)
                     /locus_tag="KI681_RS26770"
                     /old_locus_tag="KI681_26775"
     CDS             complement(3346..3669)
                     /locus_tag="KI681_RS26770"
                     /old_locus_tag="KI681_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3733..3924
                     /locus_tag="KI681_RS26775"
                     /old_locus_tag="KI681_26780"
     CDS             3733..3924
                     /locus_tag="KI681_RS26775"
                     /old_locus_tag="KI681_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165974.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4592..4705
                     /locus_tag="KI681_RS28710"
     CDS             4592..4705
                     /locus_tag="KI681_RS28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071821494.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGN"
                     /name="replication initiation protein"
     gene            4997..5626
                     /locus_tag="KI681_RS26780"
                     /old_locus_tag="KI681_26785"
     CDS             4997..5626
                     /locus_tag="KI681_RS26780"
                     /old_locus_tag="KI681_26785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_140430500.1"
                     /translation="MRHIFFLLYFFCISTIASNELKINNMPIELIFNDGSSAKISSCTD
                     LQNQMSLGKKIESIPNISDPDYNDIKNKIFICRMQNYISNHRMTRIKDSLSIHDVLSNF
                     PATAAYFISQEEMDKVSREYSNKTILEYTPDLIIDKNKAISQKKYTSYSLPEYYHFKNS
                     DGDEIKIVILAGHSIGGTASSRSYWRIDDDHSHPWTVTELDENSFL"
                     /name="hypothetical protein"
     gene            complement(5690..6451)
                     /locus_tag="KI681_RS26785"
                     /old_locus_tag="KI681_26790"
     CDS             complement(5690..6451)
                     /locus_tag="KI681_RS26785"
                     /old_locus_tag="KI681_26790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224728059.1"
                     /translation="MPGFNYGGYGDGTGWSSERGDGPAPGGGSHGNSGRDDHRGESGYG
                     YPGHNGISGPVSLRTTDSYDGDTMSGYINNLINHEGFKNTMYRDTEGNITVGIGHLLSS
                     PEMAAGLPFNRTRITHGHGDEMEHEFSVSREDITSSFNALRDNPTAISGIHISNDAVIG
                     LAISDVESTISGLKGLYSDFDSFPRSAKTALVDMGFNVGVGKLRSDFPNFNNAVNKKDW
                     NTAADESHRTKIGEGRNNDTKAQFQQAANGN"
                     /name="hypothetical protein"
     Typing          complement(4010..4091)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.59"
                     /coverage="100.0"
                     /evalue="2.9e-32"
     Typing          4108..4664
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.238"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1807..3356)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacagccaac cacaccacgt gctattcccg cccgatgcca caaaaaccag cacaaacatt
       61 accgttctcc ggcctcagta tgttttactg aaaccatgat atgagacacc aatgggacac
      121 tgtcacttca tggcatggca cacactccag gacgcactga aaatgacggg cagatcgcgt
      181 tcacagtttt accgtgatat gcgcgcaggc cttgtcagtt accgtacgag ccaggacgga
      241 cgacgggagt ttgaaaccag tgaactgatc cgggcatacg gcgaattaaa gcggaatgag
      301 acaccagaaa ggcacactgc gggacaggca gaaaaacctc ataatcagca gacagaacgc
      361 attctccggg aggtgaacga gctgaaacaa tgcctgacgc tgatgcttga ggataaacaa
      421 gcccaggata cggagcgccg gcgccaggaa gcagaacggg aacaactaca gaatgagata
      481 gtccagctca ggcaggcgct ggagcaggaa aagaaacggg gattctggtc taggttgttc
      541 ggtcgctgaa cgcgctcaga gactgatgat aagatagcca tcagataata actcaccgaa
      601 aataaatact ttaaggtaag gagactcgca tgaaacgttc tggcggcaca cgctatatgt
      661 atcttagcgc cctgataact gtgacagcag ccctcacagc ctgtacccca aaaaccagcc
      721 tggaacaaca tacccggcat tatgtttatg cttcagatga tgggtttgat cctaacttct
      781 acaccaagaa agctgacacc acacggatga tgattccgtt ctttcagcag ttccgggaga
      841 tgggggtgaa agacaaagca gccggagtat cagcagaaaa ggcacagcaa cgcataaaag
      901 aattccactc tgaagaattc cttcagtcac tgagaaccac aaccaacttt gcgggcagaa
      961 aatacacgaa cagtgatacg ccatcaccgg aaaaaatgaa gctgctggct gacacgattt
     1021 ctgcgattta tctcgatggc tacgagggaa gacagtaagt ggatttacca taatccctta
     1081 attgaacgaa ccctcaaggc tattcatcct gcccctaaat cagagtctca cacctggttt
     1141 tgattcgctc aatttttatt ctattttttt gaatgatatc cgcaaattgt ttcgatttta
     1201 atcagctcat ggtcgtccta tcatttatca catcagggca acaacgcccg atacagaaca
     1261 aaattactta aggaatacga ccatgaaatc catcaaaact ttcgttgcag ttgctgctct
     1321 atccatgatg tctttcggtg cctttgctca gagcgtcagc gctaccgcct ccaccctgga
     1381 tcgtgctgaa gcaaaaattg ccgcacaggc tgctgaacag ggtgcgtcat acaaaatcac
     1441 cagcgctcag ttcaataacc gtgtccacat gacggctgaa ctgaccaaat aagatggtcg
     1501 cagatggagc tgccgaacag gtcgccggga ggcggcccgt ttcgccacta aacaggaaaa
     1561 ccctcaagcc ccttctgggg cctttttgcg tctgttattt aacataaagg catgattgta
     1621 cgcaccgcta aaacgcgttc agcgcgatca cggcagcaga caggtaaaaa tggcaacaaa
     1681 ccacccgaaa aactgccgcg atcgcgcctg ataaatttta accgtatgaa tacctatgca
     1741 accagagggt acaggccaca ttacccccac ttaatccact gaagctgcca tttttcatgg
     1801 tttcaccatc ccagcgaagg gccatccagc gtgcgttcct gtatttccgg ctgacgctcc
     1861 cgttccaggg ataacacatg ttcgcgctcc tgtatcagcc gttcctctct tatctccagt
     1921 tctcgctgta taactggctc aagcgttctg tctgctcgct caagtgctgc acctgctgac
     1981 tcaactgcat gacccgctcg ttcagcatcg cgttgtcccg ttgcgtaagc gaaaacatct
     2041 tctgcaattc cacgaaggcg ctctcccatt cgctcagccg ctgcatatag tcctgttgca
     2101 gctgctctaa ggcgttcagc aaatgtgttt ccagctctgt cactctgtgt cactccttca
     2161 gatgtaccca ctctttcccc tgaaagggaa tcacctccgc tgatttcccg tacggaagga
     2221 caaggaattt cctgttcccg tcctgcacaa actccacgcc ccatgtcttc gcgttcagtt
     2281 tctgcaatgt ctcttcctgc ttcctgattt cttccaggtt cgcctgtatc ctccctccaa
     2341 gataccagag cgtcccgcca ctcgcggtaa acaggagaaa gactatcccc agtaacatca
     2401 tgcccgtatt ccctgccagc tttaacacgt ccctcctgtg ctgcatcatc gcctctttca
     2461 ccccttcccg gtgtttttcc agcgattcct ctgtcgaggc tgtgaacagg gctatagcgt
     2521 ctctgatttt cgtctcgttt gatgtcacag cctcgcttac agattcgccg agcctcctga
     2581 actcgttgtt cagcattttc tctgtagatt cggctctctc tttcagcttt ttctcgaact
     2641 ccgcgcccgt ctgcaaaaga ttgctcataa aatgctcctt tcagcctgat attcttcccg
     2701 ccgttcggat ctgcaatgct gatactgctt cgcgtcaccc tgaccacttc cagccccgcc
     2761 tcagtgagcg cctgaatcac atcctgacgg ccttttatct ctccggcatg gtaaagtgca
     2821 tctatacctc gcgtgacgcc ctcagcaagc gcctgtttcg tttcaggcag gttatcaggg
     2881 agtgtcagcg tcctgcggtt ctccggggcg ttcgggtcat gcagcccgta atggtgattc
     2941 accagcgtct gccaggcatc aattctaggc ctgtctgcgc ggtcgtagta cggctggagg
     3001 cgttttccgg tctgtagctc catgttcgga atgacaaaat tcagctcaag ccgtcccttg
     3061 tcctggtgct ccacccacag gatgctgtac tgattttttt cgagaccggg catcagtaca
     3121 cgctcaaagc tcgccatcac tttttcacgt cctcccggcg gcagctcctt ctccgcgaac
     3181 gacagaacac cggacgtgta tttcttcgca aatggcgtgg catcgatgag ttcccggact
     3241 tcttccggat taccctgaag caccgttgcg ccttcgcggt tacgctccct ccccagcagg
     3301 taatcaaccg gaccactgcc accacctttt cccctggcat gaaatttaac tatcatcccg
     3361 cgccccctgt tccctgacag ccagacgcag ccggcgcagc tcatccccga tggccatcag
     3421 tgcggccacc acctgaaccc ggtcaccgga agaccactgc ccgctgttca ccttacgggc
     3481 tgtctgattc aggttatttc cgatggcggc cagctgacgc agtaacggcg gtgccagtgt
     3541 cggcagtttt ccggaacggg caaccggctc ccccaggcag acccgccgca tccataccgc
     3601 cagttgttta ccctcacagc gttcaagtaa ccgggcatgt tcatcatcag taacccgtat
     3661 tgtgagcatc ctctcgcgtt tcatcggtat cattacccca tgaacagaaa tcccccttac
     3721 acggaggcat cagtgactaa acaggaaaaa accgccctta acatggcccg cttcatcaga
     3781 ggccagacat taacgctgct ggagaaactc aacgaactgg acgcggatga acaggcagat
     3841 atctgtgaat cgcttcacga ccacgccgat gagctttacc gcagctgcct cgcacgtttc
     3901 ggggatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     3961 tgagcggatg ccgggagccg acaagcccgt cagggcgcgt cagcgggttt tagcgggtgt
     4021 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttacttatg
     4081 cggtatcagt gcgaattgta tgaaaagtgc accatgcagg gtgtgaaatg ccgcacagat
     4141 gcgtaaggag aaaatgcagc ttccggcgct cttccgcttc ctcgctcact gactcgctgc
     4201 gctcggtcgt tcggctgcgg cgagcggttc tgactcacac aaaaacggta gcacggttat
     4261 ccacagaatc aggggataag gccggaaaga acatgtgagc aaaagaccag tgcaggaaga
     4321 aggccacgcc gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaatcg
     4381 acactcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4441 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgcca cttaccggat acctgtccgc
     4501 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgttggt atctcggctt
     4561 ggtgtaggtc gttcgctcca agctgggctg tatgcacgaa ccccccgttc agcccgaccg
     4621 ctgcgcctta tccggtaact atcgtctcga gtccaacccg gtaagacacg acttaacgcc
     4681 actggcagca gccactggta actgatttag aggagttggt cttgaagtta tgaaccagtt
     4741 aaggctaaac tgaaaagaca ggttttggtg actgcgctcc tccaagccag ttaccttcgg
     4801 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gttggtagcg gtggtttttt
     4861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaggatct tggcatcttt
     4921 tttactcaaa atactttcct gtttaaatac attcagtaca aaataaatta acaactaatg
     4981 taggagaacc ctaagtatgc gtcacatatt ctttttgctt tattttttct gcatatcaac
     5041 aatagcttca aatgaattaa aaattaacaa catgcctata gagttaattt ttaatgacgg
     5101 gagcagtgca aaaatatcat cttgcactga ccttcagaat cagatgtcac taggaaaaaa
     5161 aatagagtca atacctaaca tttcagaccc agattacaat gatataaaaa ataaaatatt
     5221 tatatgcagg atgcaaaatt acatatcaaa tcatcgaatg acgagaatca aagactcatt
     5281 atcaatacat gacgttttga gtaattttcc agcaacagcg gcatatttta taagtcaaga
     5341 agaaatggat aaagtttctc gtgaatacag caacaagaca attttagaat acacaccgga
     5401 tcttattatt gataaaaata aagcaatatc acaaaaaaaa tacacaagct actcactgcc
     5461 tgaatattat cactttaaaa atagtgatgg tgatgaaata aaaattgtta ttttagcagg
     5521 gcattcaatt ggtggtacag ctagctcacg ctcatactgg cgtatagatg atgatcattc
     5581 acacccctgg acagtcactg aacttgatga aaatagtttc ttataattca attaattacc
     5641 acctcataaa gaggtggtat aggcatgaaa tatgtagaat cctttatgct tagttaccat
     5701 tagctgcttg ttggaactga gctttcgtat cattatttcg gccttcacct atcttagttc
     5761 gatgagactc atcagccgca gtattccaat ctttcttatt tacagcatta ttaaagtttg
     5821 gaaagtccga tcgtaattta ccaactccca cattgaaccc catatcaact aatgcggttt
     5881 ttgctgaacg agggaaacta tcaaaatcag aataaagccc ttttaatcct gaaattgtcg
     5941 attctacatc gctaatagca agaccaatta ctgcatcgtt tgaaatgtga ataccagaaa
     6001 tagctgtagg attatctctc aatgcattaa atgaagatgt aatatcttcc cgcgatacag
     6061 aaaactcatg ttccatttca tcaccatgtc catgtgtaat tctagttcgg ttaaacggta
     6121 atccagccgc catctccggt gatgaaagca agtgaccaat accgacagtt atgttccctt
     6181 cggtatccct atacattgta tttttaaaac cttcatgatt gattaagttg ttaatataac
     6241 ctgacatagt atcaccatca taactatctg ttgtcctgag actcacaggc ccagatatcc
     6301 cattgtgacc aggataacca taaccacttt caccacgatg atcgtctcga ccactattcc
     6361 catgagaacc gcctcctgga gccggaccat caccacgttc agaactccag ccagtaccgt
     6421 caccatatcc accataatta aatccaggca taactaactc ctctttgact tgtaaaacaa
     6481 accgttaaaa acaaatactg tatatataac cactggtttt atatacagct taaaaattac
     6541 gccgctgcgt tttccctgtc aaccctgtgg attttcgttt ttgtgaaaac gatcaaaa
//
