LOCUS       NZ_CP075555             8801 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain RGF99-1 plasmid pRGF99-1-8k, complete
            sequence.
ACCESSION   NZ_CP075555
VERSION     NZ_CP075555.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18963532
            Assembly: GCF_018502585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Plasmids shapes the current prevalence of tmexCD1-toprJ1 among
            Klebsiella pneumoniae in food production chain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8801)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2021) College of Veterinary Medicine, Yangzhou
            University, No. 567, Wenchang Middle Road, Hanjiang District, Yangz,
            Yangzhou, Jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:16:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,730
            CDSs (total)                      :: 5,606
            Genes (coding)                    :: 5,448
            CDSs (with protein)               :: 5,448
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 71 of 158
            Pseudo Genes (incomplete)         :: 92 of 158
            Pseudo Genes (internal stop)      :: 41 of 158
            Pseudo Genes (multiple problems)  :: 35 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075555.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8801
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RGF99-1"
                     /isolation_source="a feces sample of swine origin"
                     /db_xref="taxon:573"
                     /plasmid="pRGF99-1-8k"
                     /geo_loc_name="China: Nantong, jiangsu province"
                     /lat_lon="32.39 N 120.56 E"
                     /collection_date="2019-05"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            join(7833..8801,1..993)
                     /locus_tag="KI681_RS26685"
                     /old_locus_tag="KI681_26690"
     CDS             join(7833..8801,1..993)
                     /locus_tag="KI681_RS26685"
                     /old_locus_tag="KI681_26690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_032448356.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            993..1724
                     /gene="mobC"
                     /locus_tag="KI681_RS26690"
                     /old_locus_tag="KI681_26695"
     CDS             993..1724
                     /gene="mobC"
                     /locus_tag="KI681_RS26690"
                     /old_locus_tag="KI681_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_094313769.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMMMD"
                     /name="mobC"
     gene            1753..2016
                     /locus_tag="KI681_RS26695"
                     /old_locus_tag="KI681_26700"
     CDS             1753..2016
                     /locus_tag="KI681_RS26695"
                     /old_locus_tag="KI681_26700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094313768.1"
                     /translation="MTNRVVAVGREALLMVAVMAGTLVVLAGNLLKLLWRVLPVLLLLY
                     GVVQFLARNPEIRDWLFLHFGQGGTAAILVGGILLCMRRLMK"
                     /name="hypothetical protein"
     gene            complement(2047..2226)
                     /locus_tag="KI681_RS26700"
                     /old_locus_tag="KI681_26705"
     CDS             complement(2047..2226)
                     /locus_tag="KI681_RS26700"
                     /old_locus_tag="KI681_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094313767.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     EKLWLAMRDHLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2295..2672
                     /locus_tag="KI681_RS26705"
                     /old_locus_tag="KI681_26710"
     CDS             2295..2672
                     /locus_tag="KI681_RS26705"
                     /old_locus_tag="KI681_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_094313766.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVMDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTAFDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(2734..3033)
                     /locus_tag="KI681_RS26710"
                     /old_locus_tag="KI681_26715"
     CDS             complement(2734..3033)
                     /locus_tag="KI681_RS26710"
                     /old_locus_tag="KI681_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094313765.1"
                     /translation="MPYEVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3023..3286)
                     /locus_tag="KI681_RS26715"
                     /old_locus_tag="KI681_26720"
     CDS             complement(3023..3286)
                     /locus_tag="KI681_RS26715"
                     /old_locus_tag="KI681_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_094313764.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESGPDKAALLQCLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(3592..3837)
                     /locus_tag="KI681_RS26720"
                     /old_locus_tag="KI681_26725"
     CDS             complement(3592..3837)
                     /locus_tag="KI681_RS26720"
                     /old_locus_tag="KI681_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4164..5849
                     /locus_tag="KI681_RS26725"
                     /old_locus_tag="KI681_26730"
     CDS             4164..5849
                     /locus_tag="KI681_RS26725"
                     /old_locus_tag="KI681_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_214301846.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARTAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5859..6116
                     /locus_tag="KI681_RS26730"
                     /old_locus_tag="KI681_26735"
     CDS             5859..6116
                     /locus_tag="KI681_RS26730"
                     /old_locus_tag="KI681_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            6201..6350
                     /locus_tag="KI681_RS26735"
                     /old_locus_tag="KI681_26740"
     CDS             6201..6350
                     /locus_tag="KI681_RS26735"
                     /old_locus_tag="KI681_26740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..8801
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6454..7032)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          994..1721
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.004"
                     /coverage="100.0"
                     /evalue="1.97e-140"
ORIGIN
        1 ctgaatctgt tccgcgatct cgaccagtac gccctgaaga atcttctggt tgccggattt
       61 aacctgagtg agaccggcga cgtggcggac cactaccgca tcagcgagca gaaagcggcg
      121 aagctgattg cggagcagtt tccgcagggg gcaaatattc agcaggtgct ggcggcggcg
      181 tacgcactgc cggaggccct gaaaaaggac gtgaaggggc tgatcaccaa gctggagaac
      241 gtggccgacc tgagcgtcct tcagacggac agcggcatcg acgtggcggg gataatcaac
      301 ggcggcggct gcctgtacgt catcggctcg atggacgatg aggcggtgat ccgggtgcag
      361 aagatgctgt ttgcccgctg cgcgcaaatc atcattgcgc gggacgagtt ccggaggtgg
      421 ccgcacgcga gcatcatgct cgatgagatt aaatatctgc tgtcgaagta cgtgctgaac
      481 gcgctgggga cgctgcgcag ccgggactgc aacctgctgc tcgcgcacca gtcgctgggc
      541 gacttcggcc agtgcgggca ggatctgccc gctgattttg tgaaaaccac ggtactggat
      601 aacacgccga tccgctggtt ctaccgggcg gccagccagg agtccgctca gtgggcggcc
      661 ggacagaccg gggagatccg ggtggacgtg gagcgccgcc gggccagccg ggaggcgggg
      721 aacgtggagc atatcagcgg ggacagcttc atccagaagg aggcgcggcc gctgtttgac
      781 gtgaacaccc tgcaacatct gcctgacggg ttcgcggtga tgaccgggct gggcgtggcg
      841 cggctggcgt tcagcagccc gctgcgtgtt gatcgccggg aaattccgct gaaaaccttc
      901 cctgtgctgg cgaaaacgga cccgctggcg gagtaccagt cggaggccgg gcggcagcgg
      961 ccgggtgatg atgattttgc ggggctgtac tgatggcact ggagcgatac aacgtcagcc
     1021 acgcgaagcg gcaggcacgg aacgcggaga aaacacggct gaccctgcgc tggctgcggg
     1081 aagaactgtg ttccacggcg gagctggttg cgcaacggct ggggattgcg gcggtacagc
     1141 cggtttaccg cttcctggac agcctggtgg cgaaagggct gctggtccgg gcgaaatacc
     1201 cggttgacgg ccgtcaggtc agcgtgtggg ggctgacgcc gcacggggtg gcgttctcgt
     1261 tcgacgagga cgagccgctg acggacatta ttccgtttca gccgtcgcgg gtgtcggcgg
     1321 cgcagctgcc gcaccggctg gccgtgcagt ccctgcgcct cgccatggag gcccgggggg
     1381 ccaccggctg gcggtacctg caccgtattg ccctgaaagg gatgaaggtg ccggacgcgc
     1441 tggcggagct ggacggcaga acggtggcgt ttgaggtgga acgcaccgtg aagagccgca
     1501 ggcgctatca ggaggtggtg tccgggtatc tgtttaaccg ccgggcgaac ggcatcgatg
     1561 aaatctggta catttgcccg gaccgggcga cgcaggtcag ggtgcaacgg gctattctgg
     1621 cggtggatga aatcgtgaac ccgcagaccg gcgaggcccg caaaacggtg gagctggacc
     1681 gggagcggct gtttgcgtgc tttaaattca tgatgatgga ctgagcttat acattcactc
     1741 agagaggttt ttatgacaaa tcgtgttgtt gccgtggggc gtgaagccct gttgatggtc
     1801 gctgtcatgg ccgggacgct ggtcgttctg gccggaaacc tgctgaaact gctttggcgc
     1861 gtactgccgg tgcttctgct gctctacggg gttgtgcagt ttctggcgag gaacccggag
     1921 atccgtgact ggctgtttct gcatttcggg cagggaggca cggcggccat tcttgtcggg
     1981 ggaatactcc tctgtatgcg ccgcctgatg aaataatgcc ggacagcacc ggctcggccg
     2041 gtgctgttat tcctccccga gatggtcccg cattgccagc cagagctttt ctgcatcctc
     2101 atgcagccgt tcgcacatgt cggccggggt atccatatcc agctcgttca gtttctcgag
     2161 caacagcagc gactggtcct taatgaattt cgccatgttg agggcggttt tttcctgctt
     2221 cgtcatgttt ctgtccttcc gggcacggtc tgcgtctgtc gggtatactg tctcaaaaat
     2281 agcggggtga gacaatggcg tgggtgacgg tgagacaggc gacggaactg acggggaaag
     2341 cccggagttc cctgtatcgg gatatggcga aaggccgcgt atcataccgt acggaagctg
     2401 atggtggtcg ggtgatggat accagcgagc ttatccgggt gtacggtgag ttaagacagg
     2461 gtgagactca cgagcgggac gggttgagac tgccggatga gactgaaaaa acggccttcg
     2521 acgcgttgat cgcggagata aaagcactca gggaggaggt ggccggactg cggcaggaaa
     2581 tgcaggccat gagactgctg gaacacaaac cggagacagc agagacaccg gccggaacac
     2641 cgcgccggtg gtggcagtgg ggaaaacgct gacgcgctgt gcttgtccaa ccggcgacgg
     2701 ccacgcaatg aattgcgccc ccgtcaccgg ttatcagcga acggtatggg gattctggcg
     2761 ctggccaacc acggccagaa cccggacttt cctggtctgg tcatcaacat catacaggac
     2821 atgctgtccc atcagactga tccggcgaac gtcctcgcca tagcgtgagt ccacttcata
     2881 gaggcgcggg aagtcggcca gtttgtccgc cgcagtgaga acgcgatcgg taacgttctg
     2941 cgcatcccac agggatgaat tttccgcgat atagtcaaac agcgtcgtta catcctcgat
     3001 ggctccctgt ttccattcca cctcatacgg catcgcgcag ttcctgataa cggccatgca
     3061 ggcgctggcg gagttgttcc gtggtcatgc ccggacgcgt gtcagcacgg gcggcggtta
     3121 ctttggcgcg aacctgatct tcatactctg cctgggccag ttcttcgtta gccaggcatt
     3181 gcaacaacgc agctttgtcc gggccacttt ccggcagtat gcggggcagg ttttcagcca
     3241 gttcccgcag gaagttggcg ttttcgtagt gtgtagtgtt gctcattgcg gagactcccg
     3301 gattaatggg gtgatatgaa gaagtttagc agggggcggt ctgaaagaat atgctcgtgt
     3361 cagtcaggct gatgaagacg aaagctggta aaaaagggct tacggcaccc attttacgtc
     3421 aaactgcacg ccatgcagtt gcgtgcatag gcatgcatta ggataaagtt taccgggcgc
     3481 gtttccggcg gttttccggg tggtttgttg cctgttttat cccgtagccg ccggaaatgc
     3541 cctcagaccg tctgagcggt gcgcgtaatg cgacgttatg gtaaataccc cttaccggcg
     3601 gcgctgtggc tgccaggtga aggtggtttc cgtagcctcg tatttctccc gacgcatttc
     3661 ctgcgcccgc ctggcttcac gttcgtcctg aatggcacga agcacccttt cccgcacctg
     3721 ttgttttacc gcctttggca gcgttttgtt ccctttcagg cccatggcca gtgcctgagc
     3781 gacaaaggcg tccgccaggc gcttttcttc agcgggagtc agtttcaggt gcgtcatagt
     3841 ggcctccggg cgtctcaggt ttcccgaaca gtttaaccgc aggtcctctc ctcatgccag
     3901 ttagctttca gatgtgtcta taccatctga aatgtaagtg tgatgaatgt cttaaataat
     3961 aaaatttgtt gccatgagaa attttcggga aaagacgcga tctgcttaca ggtggctccg
     4021 ggcgcttttt tgtgcctgaa gatgagaact aatctcatat attgacacct gaaaacggga
     4081 ggagtaaggt aataattata ctgtgtatat gtacagtatg ttggctttaa tttattgttt
     4141 taaatgtcaa agaggaaaac gatatgagtg gtggagacgg tcgaggtccg ggtaattcag
     4201 gtctgggaca taatggtggt caggccagtg ggaatgtgaa cggtacgtct ggtaaaggtg
     4261 gcccttcatc aggtggtggt acggatccaa acagcgggcc gggctggggt acgacgcata
     4321 cgccgaatgg agatattcat aactataatc cgggggagtt tggtaacggc gggagtaaac
     4381 ccggtggtaa tggtggtaac agcggcaatc atagcggtag ctctggtggc ggacagtctt
     4441 cggccactgc gatggccttc ggtctgcctg ccctggctac tccgggggct gaaggactgg
     4501 ctttatcagt ttccggcgat gcgttgtcgg ccgccgttgc tgatgtgctg gctgccctga
     4561 aggggccgtt taagtttggt ctgtggggga ttgcgatcta cggtgtgctg ccttctgaga
     4621 ttgcaaaaga tgatccgaaa atgatgtcaa aaattatgac gtcattaccg gccgatgcgg
     4681 tgacggagac tccggcaagt actttaccac tggaccaggc gacggttcgt gtcagacaac
     4741 gggttgtgga tgtggtgaag gatgagcggc agcatattgc ggttgtcgca ggtcggccaa
     4801 tgagtgtccc tgtggtggat gcgaaaccga caaaacgtcc gggggtattc agtgtgtcga
     4861 ttccgggtct cccgtctctg caggtgagcg taccgaaagg tgttccgaca gcgaaagccc
     4921 cgccaaaagg cattgttgct gaaaaaggtg attcacgtcc ggctggtttt acagccggtg
     4981 gtaactcccg tgaggccgtt attcgtttcc cgaaagagac cggacagaag ccggtttatg
     5041 tgtcggtgac agatgttctt accccggcac aggtaaaaca gcgtcaggag gaagaaaagc
     5101 gtcgccagca ggcatgggac gccgctcatc cggaagaggg gctgaaaaga gactatgata
     5161 aagcgaaagc tgagctggat gccgaagata aaaatattgc gaccttaaac agccgcattg
     5221 catcgacaga gaaggcgctc cccggtgcaa ggactgctgt tcaggaagcc gataaaaagg
     5281 taaaagaggc agaggcaaat aaagatgatt ttgtgactta taacccgcct catgaatatg
     5341 gctccgggtg gcaggatcag gttcgctatc ttgataagga tattcagaat cagaatgaga
     5401 aattaaaagc ggctcaggca tctttaaacg caatgaatga atccttatcc agagataagg
     5461 ctgcacttac cggggcgatg gagagccgga aacaaaagga gaaaaaagcg aaggatgcag
     5521 aaaataagtt aaatgaggaa aagaacaagc ctcgcaaggg gactaaagac tacggccatg
     5581 attatcatcc agtacctaaa acagaagaca taaagggact tggagagtta aagcgaggtg
     5641 atcctaaaac acctaaacaa ggtggtggtg gtaagcgggc ccgatggtat ggcgataaaa
     5701 aacgtaaaat ttatgaatgg gattcccagc acggtgagct tgaagggtat cgtgccagtg
     5761 atggcgaaca cctaggcgca tttgatccca aaacgggtaa gcaggttaaa gggccagatc
     5821 caaaacggaa cattaaaaaa tatctttgag aggtaaatat ggggcttaaa ttacgtttga
     5881 catggtttga taagaaaact gaagagttta aaggggaaga gtattctaaa gactttggtg
     5941 acgatggttc tgtcattgaa agcttgggaa tgcctttaaa ggataatatt aataatggtg
     6001 agtttgattt gaagaaagaa tgggttcctt tactacagcc attttttaaa aatcaaataa
     6061 aacctgatgt ggataattgt tatatagctt ttgattatcg ggacggtaac tggtaacaaa
     6121 aaattatcaa ggatgatttg aaatactgac ttccggtgtt cagggatgaa tgccggagtc
     6181 tttaaataca gggaaagtat atgaaaaagg taaagactat ttttcttttt atattgattg
     6241 ttgctggctt tcttctggcc gcatgtcagg caaattatat ccgggatgtt cagggcggaa
     6301 cggtggcacc atcgtcctcc tctgaactga cggggatcgc ggttcagtag aaaagatcaa
     6361 aggatcttct tgagatcctt tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac
     6421 cacctgggga ggtggtttga tcgaaggtta agtcagttgg ggaactgctt aaccgtggta
     6481 actggctttc gcagagcaca gcaaccaaat ctgtccttcc agtgtagccg gactttggcg
     6541 cacacttcaa gagcaaccgc gtgtttagct aaacaaatcc tctgcgaact cccagttacc
     6601 aatggctgct gccagtggcg ttttgcgtgc tttcccgggt tggactcaag tgaacagtta
     6661 ccggataagg cgcagcagtc gggctgaacg gggagttctt gcttacagcc cagcttggag
     6721 cgaacgacct acaccgagcc gagataccag tgtgtgagct atgagaaagc gccacacttc
     6781 ccgcaaggga gaacggcgga caggtatccg gtaaacggca gggtcggaac aggagagcgc
     6841 aagagggagc gacctaccgg aaacggtggg gatctttaag tcctgtcggg tttcgcccgt
     6901 actgtcagat tcatggttga gcctcacggc tcccacagat gcaccgaaaa agcgtctgtt
     6961 tatgtgaact ctggcaggag ggcggagcct atggaaaaac gccaccggcg cggccctgcc
     7021 gttttgcctc acatgttagt cccctgctta tccacggaat ctgtgggtaa ctttgtatgt
     7081 gtccgcagcg cccgccgcag tctcacgccc ggagcgtagc gaccgagtga gcgaggaggc
     7141 gcaatttccc cggcacctgc aagacggtca ccggacgcgc tgatgtggga tattgtcccc
     7201 tgattaagtc tttctattcc ctgacgatca attatcagga taggtgaacc atgtcggttt
     7261 ttcgctgaac attttacgtt aacctatctc tgttgctttt ttctccttgc ggagaaatgc
     7321 aagtcatccc cgctggggcg tggctgactt caggggaaaa gaccttgctt acgctggctg
     7381 gacgccaccg acgcacggtc ttttcccctc tttctggctc tgtccggctg gacgccggcc
     7441 acgccagaaa ttcacccggc ccccgtcagg ctcacgcctg accacccaca aaatccacgg
     7501 tgctggacgc acctgacgaa gcagcaaagc tgctcctgat tttgcgggtg gtcagccgct
     7561 cgctatggcg aactctgacg ggggccggaa caccggaaaa gaccgcactg gggtgccgcc
     7621 ttttcctctc acacgctggg gcgtgctcgt tgttcctccg gggctgcgcc ttttccgcat
     7681 ggggcggaaa gaggctggcc tccgcggtga aagctgaacg caatcaccac ggacgtgacc
     7741 gccgccgcca tgccttgggg catgtccgcg gcttgtgcag caggggctgc acaacacgcc
     7801 ggtaaacccg tcgtctgaag gagagagatt gcatgtttaa tacggattcg cttgcctggc
     7861 agtggcatat cccggagctg gcccggcaga tttacccgtg ggtcagctgg ctgtaccggg
     7921 agtattacag tctggccggt ttttttctgg ttatcggcct gtttttcggt ctggcagaac
     7981 tctgggccgg gcggatgcga ttcgtggggc tgagtcataa ggagcggcgg gagtatgccg
     8041 atcgctataa gcgcgataat aaccggactg cgccattttc ggtgctggcc tcgctggatt
     8101 cgcccgggcg gatggtcagg gcacggcgac atgtctggaa ctacgcatac tggctgaccg
     8161 gcgctgccgg tctggccctt ctgggggggc tggttgtcgc cgtgtttatc gagagctggg
     8221 gagaaaccgt cctgcgtccg gacctctacg cgaacgcaga ttattacctg ctgcagccgg
     8281 ctaacctgtt cgggattgcc tggtgtgccg gaggtcttgt cgcaggcgtc ctcctggcgc
     8341 ggctgctggc gttcctgctg cttgacgggc attttgcggc cgctgacgag gcggtgaacg
     8401 cgaaactgaa tcaggagagc cggcgcagca gccagcggac cggagaaatg accgacgtcc
     8461 gccacctgca tttcggggag ccggtaccgg ttaacgcgct ggccgatttt tccacggagc
     8521 aggcccggaa acagcaggcg gtgtttctgg gtaaggatga gcagggccag ccggtgctgg
     8581 tcccgcgtga tacctggcgc aagaccaaca ttcagatcct cggcctgccc gggagcggca
     8641 agagcgtgat ggccaccaac gcgctcattc gctgcgtacg cgatttcggg gacgcggtgg
     8701 tgtacttcga cccgaaaggg gacgcgtggg cgccgcacgt cttccgggcg cactgcccgg
     8761 acttcaccct gctggatctg cggccgggga aaccggcgca g
//
