LOCUS       NZ_CP075476             7542 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Escherichia coli strain NT1W25 plasmid pNT1W25-7k, complete
            sequence.
ACCESSION   NZ_CP075476
VERSION     NZ_CP075476.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16120652
            Assembly: GCF_014904145.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Li,R.
  TITLE     Distribution and genomic characterization of tigecycline-resistance
            tet(X4)-positive Escherichia coli of swine farm origin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2021) College of Veterinary Medicine, Yangzhou
            University, No. 88 South University Road, YangZhou, JiangSu 225000,
            China
REFERENCE   3  (bases 1 to 7542)
  AUTHORS   Li,Y. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2020) College of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Nanopore; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 03:36:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,722
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,374
            CDSs (with protein)               :: 4,374
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 63 of 230
            Pseudo Genes (incomplete)         :: 160 of 230
            Pseudo Genes (internal stop)      :: 39 of 230
            Pseudo Genes (multiple problems)  :: 32 of 230
            Pseudo Genes (short protein)      :: 3 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075476.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NT1W25"
                     /isolation_source="pig farm"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="pNT1W25-7k"
                     /geo_loc_name="China"
                     /lat_lon="32.01 N 120.86 E"
                     /collection_date="2018"
                     /collected_by="Yangzgou University"
     gene            join(7518..7542,1..371)
                     /locus_tag="IGI33_RS23665"
     CDS             join(7518..7542,1..371)
                     /locus_tag="IGI33_RS23665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_000634384.1"
                     /translation="MIVCAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLA
                     TLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLG
                     RKSLSFSKSVELHDKVIGHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(673..804)
                     /locus_tag="IGI33_RS23435"
     CDS             complement(673..804)
                     /locus_tag="IGI33_RS23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_161992909.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPKRHDLTPLAAATGRGCTRDY"
                     /name="replication initiation protein"
     gene            1442..1765
                     /locus_tag="IGI33_RS00010"
     CDS             1442..1765
                     /locus_tag="IGI33_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_170915627.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNTGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1974..>2402
                     /locus_tag="IGI33_RS23630"
                     /pseudo=""
     CDS             1974..>2402
                     /locus_tag="IGI33_RS23630"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2443..2934
                     /locus_tag="IGI33_RS00020"
     CDS             2443..2934
                     /locus_tag="IGI33_RS00020"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_140428842.1"
                     /translation="MSNLLTLAKDLEQQSKAQQKSTGEMLKAAFCEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMKTEAIPYGGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            2941..3156
                     /locus_tag="IGI33_RS00025"
     CDS             2941..3156
                     /locus_tag="IGI33_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3690..4226)
                     /locus_tag="IGI33_RS00030"
                     /pseudo=""
     CDS             complement(3690..4226)
                     /locus_tag="IGI33_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /name="DUF5384 family protein"
     gene            complement(4237..4800)
                     /locus_tag="IGI33_RS00035"
     CDS             complement(4237..4800)
                     /locus_tag="IGI33_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602744.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="WP_072666782.1"
                     /translation="MPKNVFFTLRNRKSHELKKTLLSIALLTASITALSGCQSNADQYA
                     ADVYDTGQLNAKQETKTVNIISVLPAKVAVDNTANKQAAQTFGAILGAVVGGVAGHNVG
                     SGSGLCTTAGAVGGGAVGAAVGTLVKDKSIVEGVSLTYKEGTKVFTSTQVGKPCQFTTG
                     LAVVISTKNNETRIQPNTTCPVKK"
                     /name="glycine zipper 2TM domain-containing protein"
     gene            <5186..5251
                     /locus_tag="IGI33_RS23440"
                     /pseudo=""
     CDS             <5186..5251
                     /locus_tag="IGI33_RS23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5523..6788)
                     /locus_tag="IGI33_RS00040"
     CDS             complement(5523..6788)
                     /locus_tag="IGI33_RS00040"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="WP_157914643.1"
                     /translation="MPNEIINIILDTNPDEINSNFFFSTKEIIGLLIIACNFLFILIGK
                     RDSKFQLRHFYIFIILFVSLLIAFKPLGLALKEINKGLSELKKNAQIIKDKKNFKWDSK
                     STINDKQTVVLFLGETNRGDYLHFNGYPKNTTPNLDKENLVSFKNTISQGAYTLVSTPM
                     ILSRKGVYDNGLFNETSIISAYKEAGYETWYVSYLSKSHIGDNEINLIANEADHYVVSD
                     VNVNTLEKILNNKSKKKLIVYKTIGSHYLYHTRYPSNYDVFKPSFNEKTYSTPSLKDKD
                     KLENSYANSELFSVDKQISDFINVLKNTDGLAYLSFISDHGTAIYDDGKSLYGGNTKGN
                     YNIAHFFWFNDLYHKQHPELIQKLSINKDKKITSECFVDTSLELSFIESKIKKGCSLLN
                     DKFIEKQRLVKNGKVYDFDQDL"
                     /name="phosphoethanolamine transferase"
     gene            7216..7491
                     /locus_tag="IGI33_RS23670"
     CDS             7216..7491
                     /locus_tag="IGI33_RS23670"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="WP_058663791.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCCKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHLKNSGRSR"
                     /name="IS1-like element transposase"
     Typing          1155..1332
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.188"
                     /coverage="82.0"
                     /evalue="9.75e-47"
     Typing          complement(697..1209)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.845"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1443..1759
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="6.609999999999999e-60"
     Typing          1756..3248
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.4"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacattgggg ctacgtcggt gctaaatcac gtcagcgctg gctgttttac gcgtatgaca
       61 ggatacggag gacggttgtg gcgcacgtct tcggtgaacg cactctggcc acactggagc
      121 gtcttctgag cctgctgtcg gcctttgagg tcgtggtatg gatgacggat ggttggccgc
      181 tgtatgaatc acgcctgaag ggaaagctgc acgttatcag caagcgttac actcagcgca
      241 ttgagcgaca taatctgaat ctgagacaac atctggcaag gctgggacgg aagtcactgt
      301 cgttctcaaa atcggtggag ctgcatgaca aggtcatcgg gcattatctg aacataaaac
      361 actatcagta agttggagtc attaccaaga accgatacgc ccctcgcgca cttctgctat
      421 tgaaaaagcc atatttaagc tcactttttt tactctgaat ataacctgtt agagaggatt
      481 ttcttgagat cgtccgtctg tcgtcgtagt ctcttatcca gaaacgaaaa aaccacctgg
      541 ggaggtggtt tttcgaaggt tcagtaagtt ggggaacttc tgaaccgtgg taacagggtg
      601 tacaagaccg ctgccaccaa attcgtcctt ccagtttagc cgtagtttgg ctctaacttc
      661 aagaactctg catcagtaat ctcttgtaca ccctctacca gtggctgctg ccagtggcgt
      721 taagtcgtgt cttttcgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      781 ggactgaacg gggggttcgt gcacacagtc cagcttggag cgaactgcct acccggagct
      841 gagtgtcagg cgtggaatga gataaacgcg gccataacag cggaatgaca ccggtaaacc
      901 gaaaggcagg aacaggagag cgcacgaggg agccatcagg gggaaacgcc tggtatcttt
      961 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgctcgtcag
     1021 gggggcggag cctatggaaa aacggcagct ccgtatcctc ttcctgtcct gcttgccatg
     1081 ccgtatgcct ttgctcttat ccgtttgcag gtaacgcccg ccgcagccga acgtcaggag
     1141 cggaagcgac cgagtgagcg aggaggcgtc atatcactgt aacctccatt ttctcctgta
     1201 cgccctgcag ctgcattttt cccctgccac atgaagcact tttattaagt gttactccgc
     1261 cgtaacatat tgagccagta tacactccgc tagcgctacg tgactggttc agggctgcgc
     1321 cccgaaaccc gccaaaagcc tgacgcgcct gcggcttgtc caaccccgcg ccgaccggga
     1381 aaggctttcc cgcccgtccc ggcgttatca ggaggccggt ttgtcagaga aacggaacaa
     1441 gatgctcacc atgtgggtga cggaggacga gcaccggcgg ctgcttgagc gctgtgacgg
     1501 caggccgctg gcggcctgga tgcggcagac ctgcctggac gaaaagcctg cgcgcaccgg
     1561 aaagctcccc tcaatttcgc cggcgctgct gcgccagctt gccggcatgg gcaataacct
     1621 gaaccagata gcccgccgtg ttaacaccgg cggcggtact ggccatgaca gggtacagat
     1681 tgtggcggcg ctgatggcca tcgatgccgg acttgagcgg ctgcggcacg ccgttcttga
     1741 gaaaggaacg gacgatgatc gttaaatttc atccacgcgg tcgcggcggt ggcgccgggc
     1801 cggtggacta tctgctgggt aaagaccggc agcgtgaagg cgccaccgtc ctgcagggga
     1861 agccggaaga agtccgggag ctgattgacg cgtcgcctta cgttaagaaa tacacctccg
     1921 gcgtcctgtc gtttgcggag gccgatctcc ctcccggcca gcgggaaaaa ctgatggcca
     1981 gcttcgagcg ggttctgatg cccggactcg ataaagatca gtacagcatt ctgtgggtgg
     2041 agcacgcgga caaggggcgt ctggagctga actttctgat ccccaatacc gagctgctga
     2101 ccggcaggcg gctccagcct tactacgacc gcgccgaccg gccgcgcatc gatgcctggc
     2161 agactatcgt gaacggcaga ctggggctgc acgacccgaa cgcgccggaa aaccgccggg
     2221 cgctggtcac gccgtccggg ctgccgaaaa cgacgcagga ggccgcagaa gccattaccc
     2281 ggggactact gagcttagcc tcgtccgggg agctgaaaag ccgtcaggat gtcactgaca
     2341 ctcttgaagg tgcaggtttt aaggtggtgc gcaccacgaa aagcagtatc agcattgccg
     2401 acccggacgg ggggcgaaac atccgactga agggagctat ctatgagcaa tcttttaacg
     2461 ctagcgaagg acttagagca gcaatcgaaa gcgcagcaga agagtactgg cgagatgctg
     2521 aaagccgcat tctgcgagca cgagcagtct gtcagagcgg aactgagcgc aagcgcgagg
     2581 agaatcagcg acgccatcct cgcccacgag cagagtatga gcgaggcgat ggagaaaaac
     2641 cggcggagcg tactgcggac tgccgggcgg acatggctca ccatcctgat ggtgtcagcg
     2701 ctgctgatcg gcacgagcgg gagcatactg tggtggcagg ggcagcagat aaccgacaat
     2761 tacacgcatc ttcgtcagca ggaagatacc ctggcgaaaa tgaccgcccg gacgtgggga
     2821 gtgcgctatc aggagagcag cgacggccgg aggtttctta tcctaccgcc ggggatgaaa
     2881 accgaagcca tcccttacgg cgggacgaca tggatccgcc tgaaacagga gtagcggaag
     2941 atgacagagc tggaaaagca gttgctgagc gcattagagc agctacagca ggactactcg
     3001 caaaggctgg acgaatggga gagcgccttc gcggaatggc ggagcatgtg tgggcttatg
     3061 caacgggaga acgcggtgct gaacgagcgc gtgtcggact tgagcacgca ggtgctgagt
     3121 ttaagcgagc agctgcgccg cttgtccggg aactgaatgc catcgagctc cggcagaggg
     3181 agaatgagta tcagaaaaca ctggagctgg agcgccagcc gcagaaaacc taccgcgggc
     3241 cgacacttta aaccatccag gcagctgaca acccgcacga agaggggctc ttacttctcc
     3301 cgctaaatag gttcgtgatt aaaaagatca cggcctatac ctggggggga attatgaaat
     3361 tgcttaacgt cattgaagct gtacttaact gcctgtatct ggcggccggg ctgtgggact
     3421 ggctggagaa gcatttacct gtttttaagg cgttcgcgct gattttagtg tgactttaca
     3481 cgccatgcat ctggctgcat ggctatgcag tcgggtaaaa tttatctgtc acgaacgggg
     3541 cggtgtatcg ggtggtttgt tgccggtttt acctgactgc cgccccgttt gtggcgaacc
     3601 cgtccggagc ggtgcgggca acagatgtta tggtaaataa catccgttgt taattaaact
     3661 tgaaatcaac tttaattata aaaatgaagc tagttaaacc attctccagc ttctttttct
     3721 ctggctttcc cttcgctttt cattaaatct ttcccgcctt ctgaaatatt tctgtttgag
     3781 ccggcgtgag attgaattac atctgtttgg gctgccttgt tttttaactc ttggtctatg
     3841 aattaatttt cgcgtttaac tcttgcctct tccctggcta atgctaactt ttgtttttcg
     3901 atttcaagct ttcttagttc atcttcatat ttctgatctc tatttttatc ggctttgagc
     3961 tcagcttcga ttctttcacg gtgagctttt ctctctgcag aagctatggc tgctctcctg
     4021 ttcgcttctg ctcttgctct ttctctcctt ttttgctcaa gccgtttgtt ttcctcaatt
     4081 ttttctctct ggatatccat gagtctttgc tcttcagctt ttccctgctg ttcagtttga
     4141 gctactgcag ataattgttc ttgaagtgag tttgcaaagc tgcttccact aaaagaaagg
     4201 atgaggggta taatgattct tttcatcgtt tgaatcttat ttttttaccg ggcatgttgt
     4261 gtttggctgg attcttgttt cattattttt tgtggaaata acaacagcta aaccagtagt
     4321 aaactgacat ggttttccta cttgagttga tgtaaatacc tttgtccctt ctttgtaagt
     4381 taaagaaaca ccttcaacga tagatttatc tttgactaat gttcctactg cggcaccgac
     4441 ggcaccaccg ccaacggctc cggctgttgt gcaaagtccg ctaccacttc cgacgttatg
     4501 accagccaca ccgccaacca cagcgccaag gattgcccca aaggtttgtg cagcttgttt
     4561 gtttgcagtg ttatcaacag caaccttcgc aggcaaaaca gaaattatgt ttacagtttt
     4621 cgtctcctgt tttgcattca gttgccctgt gtcataaacg tcagcagcat attgatcggc
     4681 attggattga cagcctgata aagcggtaat cgaggcggtt agtagggcta tgcttaacag
     4741 tgtttttttg agctcatgtg acttcctgtt tcttaatgta aaaaaaacat ttttaggaat
     4801 tatccgtaaa aagtaaaggc taaaggtggt ggttgatttt ccggtaatgg tgccaactta
     4861 ctgatttagt gcatgatggt gtttttgagg tgctccagtg gcttccattt ccatcagatg
     4921 tccttcctgg ggtcggtttg ggaaatggaa attattgtac gttaagaaca taatttaacc
     4981 gtttctgatt ggtaactgat tgatttattg ttactgacta tcgcatttaa gttattgcgc
     5041 tcacgctgtc gcatttaggt tgtctcatta cccaaacata caggctaaat gccgtatagt
     5101 gcaggagtac accctattgc gatagcgcga tctgttcaaa aaacaggctt aacgtacaat
     5161 aatttccaat ctccaaactc accccttatt cacgctggcc aacctgtttc gggcggacca
     5221 aatgatacgt cagtgggaga gatctcacta aaaactgggg ataacgcctt aaatggcgaa
     5281 gaaacggtct aaataggctg attcaaggca tttacgggag aaaaaatcgg ctcaaacatg
     5341 aagaaatgaa atgactgagt tagccgagaa gaatttcccc gcttattcgc accttcccta
     5401 actttgcaac agtgtcttat attttgtata acttagtaaa catatttaac ctgacagaca
     5461 cctaataaaa ataagtaact gccaggttaa gttttagcta gttcaattat atggaaatca
     5521 ttttataaat cttggtcgaa atcataaact ttaccatttt tcaccaatcg ctgtttttca
     5581 ataaatttat cgtttagtag ggaacaacct tttttaatct tactttcaat aaaggataac
     5641 tctaaactag tatctacgaa acattcggat gttatttttt tatctttatt aattgaaagt
     5701 ttttgtatta gctcaggatg ttgtttgtgg tataaatcat taaaccaaaa aaaatgagca
     5761 atgttatagt tgccttttgt attaccgcca tacaacgatt taccatcatc ataaatagca
     5821 gttccatggt ctgaaataaa agataaatat gcaagtccat ctgtattttt taacacatta
     5881 atgaaatcgc ttatctgttt atcaacagaa aataattctg aattagcata gctattttct
     5941 aatttatcct tatcttttaa agatggagtt gaatatgttt tttcattaaa agatggtttg
     6001 aaaacatcat aatttgatgg ataccttgta tgatacagat aatgactccc gatagtttta
     6061 taaactatga gctttttctt gcttttatta tttaatattt tctctaatgt attcacgttg
     6121 acatcagata caacataatg atcagcttca tttgcaatta aatttatttc attatctcct
     6181 atgtgacttt tactcagata agaaacatac caagtttcat atccagcctc tttataagca
     6241 ctaattattg atgtttcatt aaaaagacca ttatcataca caccttttct agaaaggatc
     6301 attggtgtgg aaactaaagt atatgctcct tgagatattg tgtttttaaa agaaactaaa
     6361 ttttctttat ctaaatttgg ggttgtgttt ttagggtaac cattaaaatg taaataatct
     6421 cctctattag tttcgcctaa aaaaagaacc actgtttgct tatcatttat tgtagattta
     6481 gaatcccact tgaagttttt tttatctttt ataatttgtg cgtttttctt aagttcagat
     6541 aaacctttat taatttcttt caaagctaat cctaatggct taaaggcgat taacaaagaa
     6601 acaaacagaa taatgaaaat ataaaaatgc cttaactgaa atttagagtc tctttttcca
     6661 ataagaataa ataagaagtt acatgctatg attagtagcc ctatgatttc ttttgttgaa
     6721 aagaaaaaat tactattaat ctcatctggg ttagtatcaa gaataatatt aatgatttca
     6781 tttggcacct gatgaaaata ccaggaggtc attcgatatg tcattaaatc aacaaataga
     6841 agtgaaataa taaaacttga tattgataaa gaaataattg aattaatttt tgaagtgtaa
     6901 ccatagaaaa aaataacacc aagtatgata agcgttaagg agtaataaat attactagcg
     6961 attattctat gggaagtatt aattataaaa attagattaa taatacataa agctatgtat
     7021 atgttttttt gatggttttt tgaaatttta ttgatgatgt tcattatttg ataacataga
     7081 tataagtaag taagtaagta agtaagtaag tttaaaaaaa aaatcatagg aaatcattat
     7141 ttaattaaaa agccgctctc ggtaatgctg ccaacttact gatttagtgt atgatggtga
     7201 tttttaggtg cttgcatggc ttccatttcc atcagatgtc cttcctgctc cgctactgaa
     7261 ggcgtggtgc gtaacggcaa aagcactgcc ggacatcagc gctatctctg ctctcattgc
     7321 tgtaaaacat ggcaactaca gttcacttac accgcctctc agcccggtac gcaccagaaa
     7381 atcattgata tggccatgaa tggcgtcgga tgtcgcgcca gtgcacgcat tatgggcgtt
     7441 ggcctcaaca cggttttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgta
     7501 tacaaccggg cagtgatgtg attgtctgcg ctgaaatgga cg
//
