LOCUS       NZ_CP075320            11969 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain DD521 plasmid pDD521.4, complete
            sequence.
ACCESSION   NZ_CP075320
VERSION     NZ_CP075320.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19073635
            Assembly: GCF_018454365.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Zhang,D.-F. and Qu,P.-H.
  TITLE     Genomic characterization of two pathogens isolated from a sputum
            sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   Zhang,D.-F. and Qu,P.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2021) Institude of Marine biology, Hohai
            University, No.1, Xikang RD, Nanjing, Jiangsu 210098, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 26-APR-2021
            Assembly Method :: SMRT Link v. 5.1.0; Unicycle v. 1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120x
            Sequencing Technology             :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 14:33:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,600
            CDSs (total)                      :: 5,474
            Genes (coding)                    :: 5,328
            CDSs (with protein)               :: 5,328
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 57 of 146
            Pseudo Genes (incomplete)         :: 91 of 146
            Pseudo Genes (internal stop)      :: 22 of 146
            Pseudo Genes (multiple problems)  :: 21 of 146
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075320.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD521"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pDD521.4"
                     /geo_loc_name="China: Lushi County, Sanmenxia City, Henan
                     Provience"
                     /lat_lon="34.05436 N 111.04782 E"
                     /collection_date="2021-01-12"
                     /collected_by="Dao-Feng Zhang"
     gene            complement(join(10719..11969,1..237))
                     /gene="ltrA"
                     /locus_tag="KJA60_RS27830"
                     /old_locus_tag="KJA60_27835"
     CDS             complement(join(10719..11969,1..237))
                     /gene="ltrA"
                     /locus_tag="KJA60_RS27830"
                     /old_locus_tag="KJA60_27835"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            881..1126
                     /locus_tag="KJA60_RS27835"
                     /old_locus_tag="KJA60_27840"
     CDS             881..1126
                     /locus_tag="KJA60_RS27835"
                     /old_locus_tag="KJA60_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1400..1771
                     /gene="tolA"
                     /locus_tag="KJA60_RS27840"
                     /old_locus_tag="KJA60_27845"
     CDS             1400..1771
                     /gene="tolA"
                     /locus_tag="KJA60_RS27840"
                     /old_locus_tag="KJA60_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1768..2133
                     /locus_tag="KJA60_RS28070"
     CDS             1768..2133
                     /locus_tag="KJA60_RS28070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            2308..2586
                     /locus_tag="KJA60_RS27850"
                     /old_locus_tag="KJA60_27855"
     CDS             2308..2586
                     /locus_tag="KJA60_RS27850"
                     /old_locus_tag="KJA60_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            2574..2888
                     /locus_tag="KJA60_RS27855"
                     /old_locus_tag="KJA60_27860"
     CDS             2574..2888
                     /locus_tag="KJA60_RS27855"
                     /old_locus_tag="KJA60_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3052..3480
                     /locus_tag="KJA60_RS27860"
                     /old_locus_tag="KJA60_27865"
     CDS             3052..3480
                     /locus_tag="KJA60_RS27860"
                     /old_locus_tag="KJA60_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            3506..3685
                     /locus_tag="KJA60_RS27865"
                     /old_locus_tag="KJA60_27870"
     CDS             3506..3685
                     /locus_tag="KJA60_RS27865"
                     /old_locus_tag="KJA60_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3712..4242)
                     /locus_tag="KJA60_RS27870"
                     /old_locus_tag="KJA60_27875"
     CDS             complement(3712..4242)
                     /locus_tag="KJA60_RS27870"
                     /old_locus_tag="KJA60_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4249..4980)
                     /gene="mobC"
                     /locus_tag="KJA60_RS27875"
                     /old_locus_tag="KJA60_27880"
     CDS             complement(4249..4980)
                     /gene="mobC"
                     /locus_tag="KJA60_RS27875"
                     /old_locus_tag="KJA60_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4980..6944)
                     /locus_tag="KJA60_RS27880"
                     /old_locus_tag="KJA60_27885"
     CDS             complement(4980..6944)
                     /locus_tag="KJA60_RS27880"
                     /old_locus_tag="KJA60_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(8428..8577)
                     /locus_tag="KJA60_RS27885"
                     /old_locus_tag="KJA60_27890"
     CDS             complement(8428..8577)
                     /locus_tag="KJA60_RS27885"
                     /old_locus_tag="KJA60_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8662..8919)
                     /locus_tag="KJA60_RS27890"
                     /old_locus_tag="KJA60_27895"
     CDS             complement(8662..8919)
                     /locus_tag="KJA60_RS27890"
                     /old_locus_tag="KJA60_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8929..10614)
                     /locus_tag="KJA60_RS27895"
                     /old_locus_tag="KJA60_27900"
     CDS             complement(8929..10614)
                     /locus_tag="KJA60_RS27895"
                     /old_locus_tag="KJA60_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3929..11969
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7747..8325
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4253..4980)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gactgtcagc aggtggaaca atgctgtaaa ccgttctttc ttccgctgct tcgcagcttc
       61 ccgcacgcgt gacagcctct gtgacatgct ttcccggctc tgtgtccggc gcatgtgtgg
      121 ctgttccgcg ttccccttgg ccccgctcct tcgctccact gactccgctc ctttcgggtt
      181 gttcgcctgc ttcgccgcta ctatgagcga gtccgacttc tcctctccgt acatcaccgg
      241 ctatgactcc tcgtcttccc ggtgcgggcc atctccgaca ctggcagatg gtcagagggg
      301 agatctcccg gttcccgcgt agagatcgta ttgacatgcc agggtctcag accccgccgg
      361 gtccatgtgg cactcgcagt atcgcaccct atgatgttgc cttccgttaa cagtacaacg
      421 tcggcacccg gtaatttaat atacatttcg tggctcaatg gctggcctgt caacacccct
      481 gtcaacgctt cgccccatac ctcgcggtat gcaacgcatg actcggggac cttgtggatt
      541 gctggtcctt caatggtcgg ggactttcac cccttgatct ctaccggtct cccggcgcac
      601 actgtacata tacacagtat aattattacc ttactcctcc cgttttcagg tgtcaatata
      661 tgagattagt tctcatcttc aggcacaaaa aagcgcccgg agccacctgt aagcagatcg
      721 cgtcttttcc cgaaaatttc tcatggcaac aaattttatt atttaagaca ttcatcacac
      781 ttacatttca gatggtatag acacatctga aagctaactg gcatgaggag aggacctgcg
      841 gttaaactgt tcgggaaacc tgagacgccc ggaggccact atgacgcacc tgaaactgac
      901 tcccgctgaa gaaaagcgcc tggcggacgc ctttgtcgct caggcactgg ccatgggcct
      961 gaaagggaac aaaacgctgc caaaggcggt aaaacaacag gtgcgggaaa gggtgcttcg
     1021 tgccattcag gacgaacgtg aagccaggcg ggcgcaggaa atgcgtcggg agaaatacga
     1081 ggctacggaa accaccttca cctggcagcc acagcgccgc cggtaagggg tatttaccat
     1141 aacgtcgcat tgcccgcacc gctcagacgg tctgagggcg tttccggcgg ctacgggata
     1201 aaacaggcaa caaaccaccc ggaaaaccgc cggaaacgcg cccggtaaat tgtaacccgc
     1261 tgcatgccta tgcacgcaac tgcatggcgg gccgtttggt gtaaaaacga tacgataagc
     1321 cgtgtttatg taaatgaacg tggccgtaaa gagtgacaaa tatacatgta acatttttta
     1381 ctgacaggtc atcaacccga tgcgtttaat accctttatc ctgctggcat tgtgcagccc
     1441 cttactcctg acggcctgta agcattccac cacccagccg ccgaaaattt atactctgga
     1501 taagtcatct aaaatcagta gctacgctgc ggccatcaag accgctgttg aaatgcagat
     1561 gtttgaacca gaggcctatg cgggaaaaat gtgcaccgtg cggatatcca tacaacgtga
     1621 tggcactctg aacagcgcca ttgctgaagg aggagatccg aaactctgta aagcagcaat
     1681 atcagccgtt acccacgcca aaattccgcc ggcaccggat gaaaatacct ggcaaatatt
     1741 caaaaacgcc cctctggatt tcagaccatg aaagctttta ctctgacagt gctggcggcc
     1801 atgttcctca ctccggtaca tgcgatacca ctaaaacccg taaccacact gcagccaacc
     1861 tacccggtac aggccgcagc aaaacgcatc gaaggtcagg tgaccgccac gtttgatatt
     1921 ctggacgatg gacgagtgga caacgtgtcg cttaccggct gtcacctttt ttttcgggaa
     1981 acgctgacgg caatgaggca ctggctatat cccgcagggc acccggcaat gcgacaggtt
     2041 ataaattttg aattcagtct gaaaaatgac ggcttacagg atccgcgacc agtcaccacc
     2101 caaaagctca atgatgatgg ttgccgccag taacaggtcc tttaacccgc aggctgcaat
     2161 agtcctggat gatgagtgag cctgtagcag ccctgattag tggaagcgaa gctgaaaaat
     2221 gggcagcaaa agccgtattt ctggcaaata gcgccgccac ggattaaagt agtcatgtgt
     2281 aatacatatg actacaaagg ggcttttatg tcatcagcaa catccatcag gctcgatgaa
     2341 gaacttaagg accggctgaa aacgctggcc gatgaccggc accgctcagc gcacgcgcta
     2401 atgctggagg caatcaccga atacatcgac cgggaagaga agcgcagcca gtacctgcgg
     2461 gacggacagg ccgcatggca gcactatcag gaaacgggtc tgcaccttac tgcggaagaa
     2521 gcggaagcct ggataagtac ctggggaact gagaacgagc aggatgctcc gccatgccac
     2581 aggtaacgat ttccgcgctg gcacagcgcg atttacaacg ccttcaggac tttctcaaaa
     2641 ccaaaaatcg gctggcagcc agaaaaggag gtgaggtgat cgtccgggct atccagcaac
     2701 tgaaaacatt gccagacatt ggccgcccgg tgccgtttct gccgctggaa tataaggaac
     2761 tggtgatcgg gtttggcgac agtggctatg tgatgctcta ccgccacgac cgggaaatgg
     2821 accagattgt gatcgtcacc gtcaggcatc agaaagaatc cgggtatccg ggggcagaca
     2881 gcctctaata accgatgacg ggggcgcaat tcattgcgtg gccgtcgccg gtttgacaag
     2941 cgcagcgcgt cagaaaacca catcctgaac ggaaaaacgg gtaaaattca gcgatacatt
     3001 taccgtgaga aattacacac ctttcacacc cgataaaatc aggggtatac agtggcgaaa
     3061 acattgctca acctttcaca ggcagcacaa gccgcgggca ttaccagaag aacgctatac
     3121 aatcatgtaa aacagggaaa ggttaccgtg tcacgtgacg ggaaaaacaa ccctgtcgtg
     3181 gatgtgtcag aactgatccg tgtatacgga aatgtgaata tccctgagaa acaaattccc
     3241 ggcatttcac accgtgaaaa tacacaaaaa aatttcccac aggaacagct tctggcaatg
     3301 caaaaggaac tggccgatct ccggcaggct gtcaccctga tgctggaaga taaaacatcc
     3361 cgggaagaag aacgccgcca gcacgatgat gagcgccgga aactccaggc cgaagtggac
     3421 agattgacaa cagaactgac acagaagaaa aaacgcttct ggtccggctg gtttagctga
     3481 gaacttaccg aagggaatgg agaacatgac gaagcaggaa aaaactgccc tcaatatggc
     3541 taaattcatt aaggatcagt cgctgctgtt gctggaaaag ctgaacgagc tggacatgga
     3601 taccccggcc gacatgtgcg aacggcttca cgaggatgcg gaaacactct ggcaggtgat
     3661 gcgggataag ctcggggaag aataacagca ccggccatgc cggtgctggc gttatttttt
     3721 gccgaacggc caccaccggc gggcgggttt gtcctccggc agttcaggca ccggcacgtt
     3781 aaaatagcgg ctgttatatt cccgcatcag ctccgctttc agcttctcgt tcaccgcgct
     3841 ttccccgtgc cgctcgctga ccacctcaat catcgcgtta atctgctccg gtgagcactt
     3901 cccggcatca accacctgca caccgttctc gatggcgaac cgctccacct ttcgatagag
     3961 gtaatcacac agcttcattc ctgtgggttt gtcccgttcc cattgcggat tttcaccggt
     4021 gaaaaagtac gtggcttttt cgtcgctgtt gagccgctcg tcatacatcc cgatggattt
     4081 catcatcgcc agatgctggg cccggtcgac aaaacagaaa acatacgccc cggtctgcca
     4141 ggcgagcacc accatgtctt ccagatgctc gctggttggc ataatgcagc gctcgccgtc
     4201 tttgtcttcc acgtcctcaa taaataccat gtacagcgcc ataatccctc actccgttgt
     4261 cataaattta aagcacgcga acagcctctc ccggtccagc tccaccgttt tgcgggcctc
     4321 gccggtctgt ggattcacga tttcatccac cgccagaatc gcccgctgta cccggacaag
     4381 cgtcggccgg tcagggcaga tgtaccagat ttcatcgatg ccgttcgccc ggcggttgaa
     4441 cagatacccg gacaccactt cctgataccg cctgcggctc ttgacggtgc gctctacctc
     4501 aaatgccacc gttctgccgt ccagctccgc cagcgcgtcc ggcactttca tccctttcag
     4561 ggccatacgg tgcaggtacc gccagccggt ggccccccgg ccctccatgg agaggcgcag
     4621 ggactgcacg gccagccggt gcggcagctg cgccgccgac acccttgacg gctgaaacgg
     4681 aataacgtcc gtcagcggct cgtcctcgtc gaacgagaac gccaccccgt gcggcgtcag
     4741 cccccacacg ctgacctgac ggccgtcaac cgggtatttc gcccggacca gcagcccttt
     4801 cgccaccaga ctgtccagga agcggtaaac cggctgtacc gccgcaatcc ccagccgtcg
     4861 cgcaaccagc tccgccgtgg aacacagctc ttcccgcagc cagcgcaggg tcagccgtgt
     4921 tttctccgcg ttccgcgcct gccgcttcgc gtggctgacg ttgtactgct ccagtgtcat
     4981 cagtaaagcc ccgcaaaatc atcatccgct tttggtccgc cgccgtcggc cggaggcagg
     5041 tactccgcca gcgggtccgt tttcgccagc accgggaagg ttttcagcga aatttcccgg
     5101 cgctcaaccc gcagcgggct gctgaacgcc agccgcgcca cgcccagccc ggtcatcacc
     5161 gcgaacccgt caggcaggtg ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc
     5221 tggatgaagc tgtcgccgct gatgtgctcc acgttccccg cctcccggct ggcccggcgg
     5281 cgctccacgt ccacccggat ctccccggtc tggcccgccg cccactgcgc ggactcctgg
     5341 ctggccgcgc gatagaacca gcggatcggc gtgttatcca gtaccgtggt tttcacaaaa
     5401 tcagcgggca gatcctgccc gcactgaccg aagtcgccca gcgactggtg cgcgagcagc
     5461 aggttgcagt cccggctgcg cagcgtcccc agcgcgttca gcacgtactt cgacagcaga
     5521 tatttaatct catcgagcat gatgctcgcg tgcggccacc tccggaactc atcccgcgca
     5581 atgatgattt gcgcgcagcg ggcaaacagc atcttctgca cccggatcac cgcctcatcg
     5641 tccatcgagc cgatgacgta caggcagccg ccgccgttga ttatccccgc cacatcgata
     5701 ccgctgtccg tctgcaggac gctcaggtcg gccacgttct ccagcttggt gatcagcccc
     5761 ttcacgtcct ttttcagggc ctccggcaac gcgtacgccg ccgccagcac ctgctgaatg
     5821 tttgccccct gcgggaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg
     5881 tagtggtccg ccacgtcgcc ggtctcactc aggttaaacc cggcaaccag cagattcttc
     5941 agggcgtact ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc cggccgcaga
     6001 tccagcaggg tgaagtccgg gcagtgcgcc cggaagacgt gcggcgccca cgcgtcccct
     6061 ttggggtcga agtacaccac agcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg
     6121 gtggccatcg cgctcttgcc gctcccgggc aggccgagga tctgaatatt ggtcttgcgc
     6181 caggtatcac gagggaccag caccggctgg ccctgctcat ccttacccag aaacaccgcc
     6241 tgctgtttcc gggcctgctc cgtggaaaaa tcggccagcg cgttaaccgg taccggctcc
     6301 ccgaaatgca ggtggcggac gtctgtcatt tctccggtcc gctggctgct gcgccggctc
     6361 tcctgattca gtttcgcgtt caccgcctcg tcagcggccg caaaatgacc atcaagcagc
     6421 aggaacgcca gcagccgcgc cagaaggacg ccggcgacaa gccctccggc acaccaggct
     6481 atcccgaaca ggttagccgg ctgcagcagg taataatctg cgttcgcata gaggtccggg
     6541 cgcaggacgg tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg
     6601 gccagaccgg cagcgccggt cagccagtac gcgtagttcc agacatgtcg ccgtgccctg
     6661 accatccgcc cgggtgaatc cagcgaggcc agcaccgaaa atggcacagt ccggttatta
     6721 tcgcgcttat agcgatcggc atactcccgc cgcgccttat ggctcagccc cacaaaacgc
     6781 attcggccgg cccagagttc agccagaccg aaaaacaggc cgataaccag aaaaaaaccg
     6841 gccagactgt aatactcccg gtacagccag ctgacccacg ggtaaatctg ccgggccagc
     6901 tccgggatat gccactgcca ggcaagcgaa tccgtattaa acatgcaatc tctctccttc
     6961 agacgacggg tttaccggcg tgatgcgcag cccccgctgc acaagccgcg gacatgcccc
     7021 aaggcatggc ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac cgcggaggcc
     7081 agcctctttc cgccccatgc ggaaaaggcg cagccccgga ggaacaacga gcacgcccca
     7141 gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc ggcccccgtc
     7201 agagttcgcc atagcgagcg gctgaccacc cgcaaaatca ggagcagctt tgctgcttcg
     7261 tcaggtgcgt ccagcaccgt ggatttttgt gggtggtcag gcgtgagcct gacgggggcc
     7321 gggtgaattt ctggcgtggc cggcgtccag ccggacagag ccagaaagag gggaaaagac
     7381 cgtgcgtcgg tggcgtccag ccagcgtaag caaggtcttt tcccctgaag tcagccacgc
     7441 cccagcgggg atgacttgca tttctccgca aggagaaaaa agcaacagag ataggttaac
     7501 gtaaaatgtt cagcgaaaaa ccgacatggt tcacctatcc tgataattga tcgtcaggga
     7561 atagaaagac ttaatcaggg gacaatatcc cacatcagcg cgtccggtga ccgtcttgca
     7621 ggtgccgggg aaattgcgcc tcctcgctca ctcggtcgct acgctccggg cgtgagactg
     7681 cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa gcaggggact
     7741 aacatgtgag gcaaaacggc agggccgcgc cggtggcgtt tttccatagg ctccgccctc
     7801 ctgccagagt tcacataaac agacgctttt tcggtgcatc tgtgggagcc gtgaggctca
     7861 accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg
     7921 gtaggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgtcc
     7981 gccgttctcc cttgcgggaa gtgtggcgct ttctcatagc tcacacactg gtatctcggc
     8041 tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aactccccgt tcagcccgac
     8101 tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cgggaaagca cgcaaaacgc
     8161 cactggcagc agccattggt aactgggagt tcgcagagga tttgtttagc taaacacgcg
     8221 gttgctcttg aagtgtgcgc caaagtccgg ctacactgga aggacagatt tggttgctgt
     8281 gctctgcgaa agccagttac cacggttaag cagttcccca actgacttaa ccttcgatca
     8341 aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag
     8401 gatctcaaga agatcctttg atcttttcta ctgaaccgcg atccccgtca gttcagagga
     8461 ggacgatggt gccaccgttc cgccctgaac atcccggata taatttgcct gacatgcggc
     8521 cagaagaaag ccagcaacaa tcaatataaa aagaaaaata gtctttacct ttttcatata
     8581 ctttccctgt atttaaagac tccggcattc atccctgaac accggaagtc agtatttcaa
     8641 atcatccttg ataatttttt gttaccagtt accgtcccga taatcaaaag ctatataaca
     8701 attatccaca tcaggtttta tttgattttt aaaaaatggc tgtagtaaag gaacccattc
     8761 tttcttcaaa tcaaactcac cattattaat attatccttt aaaggcattc ccaagctttc
     8821 aatgacagaa ccatcgtcac caaagtcttt agaatactct tcccctttaa actcttcagt
     8881 tttcttatca aaccatgtca aacgtaattt aagccccata tttacctctc aaagatattt
     8941 tttaatgttc cgttttggat ctggcccttt aacctgctta cccgttttgg gatcaaatgc
     9001 gcctaggtgt tcgccatcac tggcacgata cccttcaagc tcaccgtgct gggaatccca
     9061 ttcataaatt ttacgttttt tatcgccata ccatcgggcc cgcttaccac caccaccttg
     9121 tttaggtgtt ttaggatcac ctcgctttaa ctctccaagt ccctttatgt cttctgtttt
     9181 aggtactgga tgataatcat ggccgtagtc tttagtcccc ttgcgaggct tgttcttttc
     9241 ctcatttaac ttattttctg catccttcgc ttttttctcc ttttgtttcc ggctctccat
     9301 cgccccggta agtgcagcct tatctctgga taaggattca ttcattgcgt ttaaagatgc
     9361 ctgagccgct tttaatttct cattctgatt ctgaatatcc ttatcaagat agcgaacctg
     9421 atcctgccac ccggagccat attcatgagg cgggttataa gtcacaaaat catctttatt
     9481 tgcctctgcc tcttttacct ttttatcggc ttcctgaaca gcagcccttg caccggggag
     9541 cgccttctct gtcgatgcaa tgcggctgtt taaggtcgca atatttttat cttcggcatc
     9601 cagctcagct ttcgctttat catagtctct tttcagcccc tcttccggat gagcggcgtc
     9661 ccatgcctgc tggcgacgct tttcttcctc ctgacgctgt tttacctgtg ccggggtaag
     9721 aacatctgtc accgacacat aaaccggctt ctgtccggtc tctttcggga aacgaataac
     9781 ggcctcacgg gagttaccac cggctgtaaa accagccgga cgtgaatcac ctttttcagc
     9841 aacaatgcct tttggcgggg ctttcgctgt cggaacacct ttcggtacgc tcacctgcag
     9901 agacgggaga cccggaatcg acacactgaa tacccccgga cgttttgtcg gtttcgcatc
     9961 caccacaggg acactcattg gccgacctgc gacaaccgca atatgctgcc gctcatcctt
    10021 caccacatcc acaacccgtt gtctgacacg aaccgtcgcc tggtccagtg gtaaagtact
    10081 tgccggagtc tccgtcaccg catcggccgg taatgacgtc ataatttttg acatcatttt
    10141 cggatcatct tttgcaatct cagaaggcag cacaccgtag atcgcaatcc cccacagacc
    10201 aaacttaaac ggccccttca gggcagccag cacatcagca acggcggccg acaacgcatc
    10261 gccggaaact gataaagcca gtccttcagc ccccggagta gccagggcag gcagaccgaa
    10321 ggccatcgca gtggccgaag actgtccgcc accagagcta ccgctatgat tgccgctgtt
    10381 accaccatta ccaccgggtt tactcccgcc gttaccaaac tcccccggat tatagttatg
    10441 aatatctcca ttcggcgtat gcgtcgtacc ccagcccggc ccgctgtttg gatccgtacc
    10501 accacctgat gaagggccac ctttaccaga cgtaccgttc acattcccac tggcctgacc
    10561 accattatgt cccagacctg aattacccgg acctcgaccg tctccaccac tcataccgtt
    10621 ttcctctttg acatttaaaa caataaatta aagccaacat gtcgcggtag ggatacccgt
    10681 taccggatac cccccgcaca gatcccggcg tgcgcgattt acgcaccggg ctcctgcctc
    10741 gggtgtctgg cggtgaaccg ctccacaggc catggatgaa gaacccgaac ccttggtagc
    10801 catgcggctg ccagtttgtt tgctttcgtc caggtcgtat catccttctg gctcctgcgc
    10861 ctgagcgccc ggcgccagag gtttgttacg tgtgtcctga acttctgcat ggtggggaag
    10921 ttgcccggta ccgagtgata gttcaggtat ccctgaacca ctctcctgag ccattttccc
    10981 tgttcgggga ttgagtaatg ccagcgcctt cgcagcccgt ctttgatggc tttcagagtt
    11041 gccgtcatcc gatcccggcg ggtctttcgt atcagcatga acctgccgtt gcgatctttc
    11101 ccgctgatgt gcgtgaaccc gaggaagttg aacgtttctg gtttgccttt tcccctgatg
    11161 gcacggtttt cggcagcgaa gcggccgaac tccatcagac gggttttctc cgggtgaacc
    11221 gtgagtccga actccctcag tctgcgctgc atggctatac ggaagcgccg ggcatcgtat
    11281 cgtttgtcga acccgatgac gatgtcatcg gcgtatctga ccattaccac attgcctgtg
    11341 gcatagcgac gtcgccactg atgcgcccac agatcgaata cgtagtggag gtatatgttt
    11401 gccagcagcg gtgagatgac cgcaccctgt ggggtgcctt cctccgttgc tcgccattga
    11461 ccctcctccg acgtcccggc tgtgagccac ttacgtatga gcctgattac cctccggtcg
    11521 ccgatccgat gctctgtgaa cctgatcagc cattcgtggc tcaccctgtc gaagaactga
    11581 ctgatgtcgg catccagtac ccagtttacg ttagtgcgta ccagccctgt ggccagtgcg
    11641 tccagtgcat cgtgctggct tcgcccgggt ctgaacccgt atgagaaccc cataaagtcg
    11701 ttttcataga ctgcgttcag gattttcacc agcgcatact ggacgatctt gtcctccagc
    11761 gaggcgatgc cgagcgggcg ttgttttcca tccgcttttg ggatgtagtg acgcctgccg
    11821 ggctgcgccc tgtagctgcc ctgatgtagc ctccggtgca gatctgttat gttgttcttc
    11881 atgtttccgg cgtagtccat ccacctgatg ccatccactc cggcggccgc tttcctgctc
    11941 agggagagga atgcggcttc cagtgcttc
//
