LOCUS       NZ_CP075285             6789 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain YTF44-1 plasmid pYTF44-1-6k, complete
            sequence.
ACCESSION   NZ_CP075285
VERSION     NZ_CP075285.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19107302
            Assembly: GCF_018449065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Plasmids shapes the current prevalence of tmexCD1-toprJ1 among
            Klebsiella pneumoniae in food production chain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2021) College of Veterinary Medicine, Yangzhou
            University, No. 567, wenchang middle road, hanjiang district, yangz,
            yangzhou, jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 12:29:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,717
            CDSs (total)                      :: 5,592
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 53 of 136
            Pseudo Genes (incomplete)         :: 82 of 136
            Pseudo Genes (internal stop)      :: 17 of 136
            Pseudo Genes (multiple problems)  :: 15 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075285.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="YTF44-1"
                     /isolation_source="a feces sample of swine origin"
                     /db_xref="taxon:573"
                     /plasmid="pYTF44-1-6k"
                     /geo_loc_name="China: Yangzhou, jiangsu province"
                     /lat_lon="32.40 N 119.40 E"
                     /collection_date="2020-07"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            196..309
                     /locus_tag="KI725_RS00015"
                     /old_locus_tag="KI725_00015"
                     /pseudo=""
     CDS             196..309
                     /locus_tag="KI725_RS00015"
                     /old_locus_tag="KI725_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            530..1201
                     /locus_tag="KI725_RS00020"
                     /old_locus_tag="KI725_00020"
     CDS             530..1201
                     /locus_tag="KI725_RS00020"
                     /old_locus_tag="KI725_00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_121560299.1"
                     /translation="MKSPHLISTQAAFFFEDTINRPDKLFNKINSDIGELIDSMPETIN
                     LPPIAPADIPRVVGHSSNGEYELSVSLNRVDIKKQYADPLLDQGSIEKFRAICKLIILS
                     VSSDMGISRIGLVSNLYIESSNPSLEISRKYLKKEDPNLLEINLRTNKESKEFGLSFNN
                     IILIQMGNVQSPSYNGKAIIVQVDTNTIETGGCIQKDIITDIFIKKSSIYNPATILERI
                     Q"
                     /name="hypothetical protein"
     gene            1201..1611
                     /locus_tag="KI725_RS00025"
                     /old_locus_tag="KI725_00025"
     CDS             1201..1611
                     /locus_tag="KI725_RS00025"
                     /old_locus_tag="KI725_00025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_121560301.1"
                     /translation="MGWFGGKKDDSPSVSVRVYTTSTNTNTDTSSSSSSTNIAGDAFWE
                     RIDDLIEQKVNNNYKPKTKKEMDEESDRNIKKEAISIIKWTLIITLSGVVITFMYCTYQ
                     GKDIPEFAKTIFTVASTLAAGAGAFVFGNKSK"
                     /name="hypothetical protein"
     gene            1775..2017
                     /locus_tag="KI725_RS00030"
                     /old_locus_tag="KI725_00030"
     CDS             1775..2017
                     /locus_tag="KI725_RS00030"
                     /old_locus_tag="KI725_00030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(2362..2763)
                     /locus_tag="KI725_RS00035"
                     /old_locus_tag="KI725_00035"
     CDS             complement(2362..2763)
                     /locus_tag="KI725_RS00035"
                     /old_locus_tag="KI725_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2763..2954)
                     /locus_tag="KI725_RS00040"
                     /old_locus_tag="KI725_00040"
     CDS             complement(2763..2954)
                     /locus_tag="KI725_RS00040"
                     /old_locus_tag="KI725_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3369..3482
                     /locus_tag="KI725_RS28805"
     CDS             3369..3482
                     /locus_tag="KI725_RS28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_100757171.1"
                     /translation="MHEPPVQSDCCVFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(3689..4165)
                     /locus_tag="KI725_RS00045"
                     /old_locus_tag="KI725_00045"
     CDS             complement(3689..4165)
                     /locus_tag="KI725_RS00045"
                     /old_locus_tag="KI725_00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(4165..4809)
                     /locus_tag="KI725_RS00050"
                     /old_locus_tag="KI725_00050"
     CDS             complement(4165..4809)
                     /locus_tag="KI725_RS00050"
                     /old_locus_tag="KI725_00050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044816498.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            complement(4811..5035)
                     /locus_tag="KI725_RS00055"
                     /old_locus_tag="KI725_00055"
     CDS             complement(4811..5035)
                     /locus_tag="KI725_RS00055"
                     /old_locus_tag="KI725_00055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            complement(5898..6320)
                     /locus_tag="KI725_RS00060"
                     /old_locus_tag="KI725_00060"
     CDS             complement(5898..6320)
                     /locus_tag="KI725_RS00060"
                     /old_locus_tag="KI725_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6320..6511)
                     /locus_tag="KI725_RS00065"
                     /old_locus_tag="KI725_00065"
     CDS             complement(6320..6511)
                     /locus_tag="KI725_RS00065"
                     /old_locus_tag="KI725_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044816495.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..313
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.527"
                     /coverage="87.0"
                     /evalue="3.03e-139"
     Typing          3076..3482
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.665"
                     /coverage="99.0"
                     /evalue="2.15e-165"
     Typing          6633..6789
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.711"
                     /coverage="99.0"
                     /evalue="8.95e-60"
ORIGIN
        1 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
       61 ctgatggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
      121 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
      181 tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgc ctaatccggt
      241 aactatcgtc ttgagtccaa cccggaaaga cacgacaaag cgccactggc agcagccact
      301 ggtaactgat ttagaggagt tagtcttgaa gttatgtgcc agttaaggct aaactgaaag
      361 aacgaatttg gttactgcgc tcctccaagc cagttacctc ggttcaaaga gcgccaactc
      421 ctcgaacctt cgaaaaaacc gcccaccaag gcggtttttt cgtattggag actaccctct
      481 cagatatgct atcgtcaagg gtattaacaa ataacaatag aggtaaaaaa tgaaatcccc
      541 tcacctaata tctactcaag cagctttttt ctttgaagat acaataaaca gacctgataa
      601 gttattcaat aaaatcaact cggatattgg tgaattaata gattcaatgc ctgagacaat
      661 aaaccttcct cctatagccc ctgcggacat tcctagagta gtgggtcact catcaaatgg
      721 tgaatatgaa ttaagcgtat cactcaatcg agtggatata aaaaaacaat atgccgaccc
      781 attgctcgac caaggttcaa ttgaaaaatt cagagcgata tgcaaactta taatactatc
      841 ggtatcttca gatatgggta tatctcgaat tgggttagtt agcaaccttt acattgaaag
      901 cagcaatccc tctttagaaa tatcgaggaa atatttaaag aaagaagatc caaacctcct
      961 tgaaataaac ctaagaacga ataaagagag taaagaattt ggcctgagct ttaataatat
     1021 aattttaatt cagatgggaa atgtacagtc cccaagctat aacggcaaag caatcattgt
     1081 acaagttgac accaatacga ttgaaacagg tggctgcata caaaaagata taattactga
     1141 catatttatt aaaaaatcat ccatatataa ccctgctaca attttagaaa ggatacagtg
     1201 atgggatggt ttggtggaaa aaaagatgac tcaccatctg tgtctgtaag agtatatacc
     1261 acatcaacaa acaccaacac agatacctct tcttcttcat catcaacaaa tatcgcaggt
     1321 gatgcgttct gggagaggat tgacgacctc attgaacaaa aagtaaacaa caactataaa
     1381 ccaaagacaa aaaaggaaat ggatgaggaa agtgacagaa acataaaaaa agaggctatc
     1441 agcataataa aatggacatt aataataact ctaagtgggg ttgttattac attcatgtac
     1501 tgcacatatc aagggaaaga tatccctgag tttgcaaaaa caatatttac tgttgccagc
     1561 acacttgctg caggtgctgg tgcgtttgtc tttggtaata aatcaaagta gaaaacgaga
     1621 ggttgattta acataactac ttaattgtac gcaccgctaa aacggcctca ggacgtttct
     1681 gacgggaaac cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc
     1741 tctgttttta cggcatgcat gagtatgcgc gcaagtgcat agcgcggaaa ttcacaccat
     1801 tcagccaggg gaagtcctgt ttccggcgtt cttctcctcc gaaaacggtg aacggggacg
     1861 caatttattg cgtggccgtg aagcgttgcc ggcgcagccg gaaaaccggt tttcagcacc
     1921 ttcaggaatc gggttcgtcg acggccggca atcacgttat ggcaagtcgc cggacgactc
     1981 accacacgat tcaccggcga ttcgaatcgt gaattgaatc aagagtcacc atgagcaaaa
     2041 aacacccaaa gtgatgaatt gaatcaagag tcgattcatc ataacaatat gataataaag
     2101 cgtgattcac atatgaatcg tgggcaattc gtcagcgatt cgccatagag tcgtccacga
     2161 cttggatctg cactttgtaa cacatcaccg cacatttatt tttcattaac ccattgttgt
     2221 aaataaggaa tctgcaaatt ttaattttgc gtggggtgtg ggtattttta gtggtgagga
     2281 gcgttcagag atctgacgat ttttgggaag gttcaggaac tccctgggat ggccggagag
     2341 gaaaactgaa gcatgacggg gtcaggattt tttccagaat ttccaccatg gcgccgggga
     2401 ttcagttaat tttgcctggt gattttcaag aaggagcatg gcctgtttca ggctgtcaat
     2461 gtgcgaatcc ttggacaaaa gtaaggtgtt caggtgttca atctcctgtt tcagatgacg
     2521 gatcacctgt tcactctggt cagcagaggc tgtatcacgc tgtccagaga cctgtccaga
     2581 atggtgttca cccttcggtt gctcaaaggg gccaaagaca cgtaaaagct ctgaagtatc
     2641 taccattttt gcattgcgct caccgctaca ggtggacagg tcacctgctt taattaacct
     2701 gtgcagtgtt gttctactct tacccgtcag tcgggcagcc tcactaatag acactctggc
     2761 catcagttac catcctgtaa tgcatccagt ctcgaggaca gtgtacggaa aagatgctcc
     2821 gcatcatcgt ggagtttctc acaaaggtcg gcctcgacat cgagatcaag ctcgttaagc
     2881 ttctccagca ggagcagaga ctgattctgg atgaatttag ccatgttcag cgctgttttc
     2941 tgttgtttcg tcataagagg cctcagagcg tcagtggtca gtgtagggat ctcctctttt
     3001 ccgcttcctc gctcactcag tcgctacgct cctgccgttc ggctgcggcg agcggcagga
     3061 gcatgcgtag aaacgctcga gaaaaccgag taatcgcatg gaggaaagaa ggaaaggccg
     3121 cacagcggcc gtttttccat aggctccgcc cccctgacga gcatcacgga atctgacgct
     3181 caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccctggtgg
     3241 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     3301 gccgcgttta tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     3361 tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgtcttttcc ggtaactatc
     3421 aacttgagtc caacccggaa agacacgacg aagcgccact ggcagcagcc actggtaact
     3481 gatttagagg agttagtctt gaagttatgt gccagttaag gctaaactga aagaacgatt
     3541 ttggttactg cgctcctcta actagccagt taccttggtt catagagccg caaactctta
     3601 gaacctaaga aaaaccgccc cgcaaaggcg gtttttcttt ttcaagaaga gtactaggtc
     3661 acacttgatg acgtcaagac ggttttcgct aaagaggaac aaccacgcca ccttcatttc
     3721 tttgaaaaaa agaggataat tccaaaatag ttgtagtcac ctcaatcaaa gtaaattcat
     3781 cattaatatc aaataaatac cacaaaccca agtgagggta accttcgtcc tttatatcaa
     3841 tacatttatc tggtttgtta acttgctcga tcacttccgc tgcatcaaat gagagatata
     3901 tggcaggttt ggttttccct acagggattc ttttatgtac atcccttacc attccttcat
     3961 aataatcttt atttgaatga taaatagaca cataatcctc tgaaatttcc tccctaagat
     4021 aaaaacactt aatagttgta gcgccaaact catctttatg atcttccata acataacgcc
     4081 ataaatatga attatttgct ctgcccgtaa atgcattaat acaagggatt gatttgaggt
     4141 tatttaaagt tgataacaca gtcatcacca ctccatcatt ttaaataatg atttgatagc
     4201 aatggagtta tcaaggctat cgccaatata tgccctacct gtaaccttaa ccactccaga
     4261 aacttttcta acaaaggaaa aaataatctc gttatttgtt ttcactatta aatttagcgt
     4321 gcctttgctt ttcttagttt gtttatatat aagcccaaaa cactctgatt ttttatcaaa
     4381 gtaaatctga gcgtcttttt ttgcagcaag ttcaggggca tacctaatca actgaacaat
     4441 agcctgcaaa gatggttcta ggtcaccagt gcagaactcg tattctctat tataaacaga
     4501 aatggcactt cttatttttt taaaaaacaa taagaattca cctgataatt ctaatgattc
     4561 gtaatgtttt aaaattgact cataaccgtc accagagcaa tctaatgttt taacatcatc
     4621 attccaagac tcccaaacag aattctcaac aacaacaaat gctcgaccca aaactttaaa
     4681 caaatccgga tctcttaaat catttagctc taacttatta taatcagaaa catcataacg
     4741 aacaccgaga tcaatgctat cattagtaat ataataatct tcactatcgc agtggttaat
     4801 gtatttcata ctaatcacct gactcaacga atttattcaa tccatcgata aggttctttg
     4861 catgtttttt ggttagagct atggaataaa aaacccttcc tgattctttt tcaggaaagt
     4921 ccactaactc taaaaaacaa atatcttcag ctatcccaac tcttagagcc atcgggaata
     4981 caggttcatt ttttcttggt gttacttttc tttctaaaga atctttattg ctcattataa
     5041 taccccgata gaaataaatt taagtttaga acataaaaaa taataccata aaacaacatc
     5101 tttcatccac aatgctcatt taacattaca ttggtcactt tatggaattt tgtggccatg
     5161 atttaacata acaccaaatt gtacgcaccg ctaaaacggc ctcagggcgt ttctgacggg
     5221 aaaccggtaa aataggcaac aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt
     5281 tttacggcat gcatgagtat gcacgcaagt gcatagcgcg aaaattcacg ccattcagcc
     5341 agggtaagtc ctgtttccgg cgttcttccc atccgaaaac ggtgaacggg gacgcaattt
     5401 attgcgtggc cgtgaagcgt tgccggcgca gccggaaaac cggttttcag cacgttcagg
     5461 aatcgggttc gtcgacggcc ggcaatcacg ttatggcaag tcgccggacg actcaccaca
     5521 cgattcaccg gcgactcttc actagagtcg tacccagagt cgtcacgtct aaaaaacaac
     5581 caaacgggga gtcgggtcta gagtcgggtc gttgttaact tattgttttt atagtcctga
     5641 gtcgttaccg actctatgac gactcgccgg ggactctatg acgagtcgtc atagagtcgg
     5701 atctgtataa ataaaattaa aaaaacgaaa tttatagaac gtaaactact gatataaaat
     5761 acaaatctgc aaaattttaa ttttgcgtgg ggtgtggata tttttagtgg tgaggagcgt
     5821 tcagagatct ggcgattttt gggaaggttc aggaactccc cggaatggcc ggagaggaaa
     5881 actgaagcat gacggggtca ggatttcttc cagaatttcc accacggaga atgaacctgt
     5941 ggagagggtt cgagcttgtg ttccagaagc tgcatagcct ggcgaagaga atcgatatgt
     6001 tcatcacgag ctttaacagc ccctttaaga ccatcgtttt cagcttcaag caaagcaatt
     6061 ttatgctgta aatcatgtaa accagagatg ttaacccctg tagcgcctgc gtcacctttt
     6121 acatcatgtt ttacagtgtc gcccccacca taaagcgagc caaaaaccct aataagctct
     6181 gatgtatcaa caactttagt atttttccca tgctttacaa cagaggtgta acttagtttc
     6241 ccactgttaa tcatcttgta aattgtgggg cgactaacct taacaagccg agccgcttca
     6301 ctaatagaca ctctggccat cagtttccat cctgtaattc atccagtccc gaagacagtg
     6361 tacggaaaag atgctccgca tcatcgtgga gtttctcaca aaggtcggcc tctacatcaa
     6421 gatcaagctc gttaagcttc tccagcagga gcagagactg attctggatg aatttagcca
     6481 tgttcagcgc tgttttctgt tgtttcgtca taagaggcct cagagcgtca gtgctcagtg
     6541 tagggatgtt ctcttttccg cttcctcgct cactcagtcg ctacgctcct gccgttcggc
     6601 tgcggcgagc ggcaggagca tgcgtataaa agctcgagaa aaccgagcga tcgcgtgatg
     6661 gggagaagga gaggccgcac agcggccgtt tttccatagg ctccgccccc ctgacaagca
     6721 tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
     6781 ggcgtttcc
//
