LOCUS       NZ_CP075260             2937 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SZP4-9-2 plasmid pSZP4-9-2-2k, complete
            sequence.
ACCESSION   NZ_CP075260
VERSION     NZ_CP075260.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19107292
            Assembly: GCF_018449045.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Plasmids shapes the current prevalence of tmexCD1-toprJ1 among
            Klebsiella pneumoniae in food production chain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2021) College of Veterinary Medicine, Yangzhou
            University, No. 567, Wenchang Middle Road, Hanjiang District, Yangz,
            Yangzhou, Jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 12:51:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,871
            CDSs (total)                      :: 5,744
            Genes (coding)                    :: 5,617
            CDSs (with protein)               :: 5,617
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 46 of 127
            Pseudo Genes (incomplete)         :: 82 of 127
            Pseudo Genes (internal stop)      :: 17 of 127
            Pseudo Genes (multiple problems)  :: 15 of 127
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075260.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SZP4-9-2"
                     /isolation_source="pork"
                     /db_xref="taxon:573"
                     /plasmid="pSZP4-9-2-2k"
                     /geo_loc_name="China: Shenzhen, guangdong province"
                     /lat_lon="22.62 N 114.07 E"
                     /collection_date="2019-05"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            153..530
                     /locus_tag="KI741_RS28195"
                     /old_locus_tag="KI741_28200"
     CDS             153..530
                     /locus_tag="KI741_RS28195"
                     /old_locus_tag="KI741_28200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110982627.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     gene            587..892
                     /locus_tag="KI741_RS28200"
                     /old_locus_tag="KI741_28205"
     CDS             587..892
                     /locus_tag="KI741_RS28200"
                     /old_locus_tag="KI741_28205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058876158.1"
                     /translation="MMKVGNVREYGPTKSHLLLMVTTLPAGVGVFIYPIVEVIWGDLSL
                     LKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIAGLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(1619..2041)
                     /locus_tag="KI741_RS28205"
                     /old_locus_tag="KI741_28210"
     CDS             complement(1619..2041)
                     /locus_tag="KI741_RS28205"
                     /old_locus_tag="KI741_28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004207907.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAISTEVNTIGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHDPSSPAHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2041..2232)
                     /locus_tag="KI741_RS28210"
                     /old_locus_tag="KI741_28215"
     CDS             complement(2041..2232)
                     /locus_tag="KI741_RS28210"
                     /old_locus_tag="KI741_28215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004207910.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2657..2800
                     /locus_tag="KI741_RS29560"
     CDS             2657..2800
                     /locus_tag="KI741_RS29560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_085409091.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGNGFAEQRNV
                     EV"
                     /name="replication initiation protein"
     Typing          2400..2765
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 aagagattac gacgcaacag aaaggatctc aagaggatcc tattccatcc ctctcccccc
       61 caaagggaaa ccagcaccac tccacgacac cagttctttg aagaaaatta ttgaacccct
      121 tgattttaat aataaaaaaa acagatataa tcatgatcca caatcaccca acttgggtaa
      181 taaaatcaat gatttatagg ttaggtattt tatcaatatc gctgcttaca gttggttgtg
      241 tttctaccag caacgttctt gagttaggta gggatacata tacggtttca gcaactgctg
      301 atggttttag agatgcggca tcagcacgtc aaagtgcctt taaaacaggt tctgagaaat
      361 gtaatagcct cggtaaaaaa ttcctgctca ctaacgaaaa cgtggctaac acaagaatgg
      421 ggatagatac cacagtaaca ctctacttcc gttgtcttga cgaaagagac agcgagtatg
      481 tacgaccaaa tctacagaaa gcacctgatc tacgcatcga gagtaaataa ttatgaattt
      541 ccttatgatc atgatcctat ggtggatatc gtcattcatc acacacatga tgaaagttgg
      601 aaatgtcaga gagtacggtc ccactaaaag ccatttactg ttgatggtta ctacgcttcc
      661 tgctggcgtt ggtgttttta tctatccgat agtagaggtg atatggggag atctgtcgtt
      721 actgaaagtt gctggttata cctttttatc cctcattgtt ggcggacttc ttgccaatat
      781 cattgttcgc ggattatctg cgacaacatt cggcattttg ctcgtaaatc cattagcaat
      841 gatatgtagc attgccggac tgatagtcct tgctatccaa catttaccat aacatccgtt
      901 atcagcaccg cctgagaggc cctcagcgcg tttctgacgg gaaaccggta aaacaggcaa
      961 caaaccaccc gaaaacccgc cagaaacgcg caggctctgt ttttacggca tgcatgagta
     1021 tgcgcgcaag tgcatagcgc ggaaattcac accattcagc cggggtaagt cctgtttccg
     1081 gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg
     1141 ttgccggcgc agccggaaaa ccggttttca gcacgttcag gaatccggtt cgtcgacggc
     1201 cggcaatcac gttatggcaa gtcgccggac gactcaccac acgattcacc ggcgactctt
     1261 cactagagtc gtacccagag tcgccgcgtc taaaaaacaa ccaaacgggg agtcgtgtct
     1321 agagtcgggt cgttattaac ttattgtttt tatagtcctg agtcgtcacc gactctatga
     1381 cgactcgccg gcgactctat gacgagtcgt catagagtcg gatctatata aataaaattt
     1441 aaaaagcgaa atttcaggaa agtaacccac tgatattaaa tacaaatctg aaaaatttta
     1501 attttgcgtg gggtgtgggt atttttagtg gtgaggagcg ttcagagatc tggcgatttt
     1561 tgggaaggtt caggaactcc ccgggatcgc cggagagcaa aactgaaaca tggcaggatc
     1621 aggatttttt ccagaatttc caccacggag aatgagccgg tgaagagggg tcgtgcttgt
     1681 gctccaaaag ctgcatagcc tggcgaaggg aatcaatatg ctcatcgcgt gctttaacag
     1741 cccctttaag accatcgttt tcagcttcaa gtaaagcgat tctatgttgt aaatcatgta
     1801 aaccaatggt gttaacctct gtagaaattg cgtcactttt tacatcatat tttacagtat
     1861 cagttacacc atcaagagaa ccaaacaccc taataagctc ggatacatca ataactttaa
     1921 tatccttccc gtgctttaca acagaggtat aacttaactt cccgctgtta atcatcttgt
     1981 aaatagtggg acgactaacc ttaacaagcc gtgcagcttc actaatagac actctggcca
     2041 tcagttacca tcctgtaatg aatccagtct cgaggagagt gtacggaaaa gatgctctgc
     2101 atcatcgtgg agtttctcac aaaggtcggc ctcagcatca agatcaagct cgttaagctt
     2161 ctccagcagg agcagagact gattctggat gaatttagcc atgttcagcg ccgttttctg
     2221 ttgtttcgtc ataaagggcc tcagagcgtc agtgctcagt gtaaaacctg tctcttttcc
     2281 gcttcctcgc tcactcagtc gctacgctcc ggtcgttcga ctgcggcgag cggcaggagc
     2341 atgcatagaa aagctcgaga aaaccgagcg gtcaaaggag gaaaaggagg aaaaaaagga
     2401 aaggccacgg cgttgccgtt tttccatagg ctccgccccc ctgacaagca tcgcagaaac
     2461 ggacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2521 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcactccg
     2581 ctgttatggc cgcgtttatc tcattccacg cctgacactc agttccgggt aggcagttcg
     2641 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactgctgcg ccttatccgg
     2701 taactatcgt cttgagtcca atccggaaag acacgactta tcgccactgg cagcagccac
     2761 tggtaacgga ttcgcagaac aaagaaatgt tgaagtgtga ggtgaagtac agctacactg
     2821 taataactaa tttggtttct gtgctctgcg aaagccagtt acctcggttc aaagagttgc
     2881 tgctctaaga accttcgaaa aaaccgcctt gctgggcggt tttttcgttt ccagggc
//
