LOCUS       NZ_CP075259             3364 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SZP4-9-2 plasmid pSZP4-9-2-3k, complete
            sequence.
ACCESSION   NZ_CP075259
VERSION     NZ_CP075259.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19107292
            Assembly: GCF_018449045.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Plasmids shapes the current prevalence of tmexCD1-toprJ1 among
            Klebsiella pneumoniae in food production chain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2021) College of Veterinary Medicine, Yangzhou
            University, No. 567, Wenchang Middle Road, Hanjiang District, Yangz,
            Yangzhou, Jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 12:51:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,871
            CDSs (total)                      :: 5,744
            Genes (coding)                    :: 5,617
            CDSs (with protein)               :: 5,617
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 46 of 127
            Pseudo Genes (incomplete)         :: 82 of 127
            Pseudo Genes (internal stop)      :: 17 of 127
            Pseudo Genes (multiple problems)  :: 15 of 127
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP075259.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SZP4-9-2"
                     /isolation_source="pork"
                     /db_xref="taxon:573"
                     /plasmid="pSZP4-9-2-3k"
                     /geo_loc_name="China: Shenzhen, guangdong province"
                     /lat_lon="22.62 N 114.07 E"
                     /collection_date="2019-05"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            complement(<118..198)
                     /locus_tag="KI741_RS29405"
                     /pseudo=""
     CDS             complement(<118..198)
                     /locus_tag="KI741_RS29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            634..825
                     /locus_tag="KI741_RS28175"
                     /old_locus_tag="KI741_28180"
     CDS             634..825
                     /locus_tag="KI741_RS28175"
                     /old_locus_tag="KI741_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042634516.1"
                     /translation="MNKQQKRAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1655..1867
                     /locus_tag="KI741_RS28180"
                     /old_locus_tag="KI741_28185"
     CDS             1655..1867
                     /locus_tag="KI741_RS28180"
                     /old_locus_tag="KI741_28185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_089634954.1"
                     /translation="MSNIKPDSLIRITKRKNENGDETTDLEWKPGSKEAIATGAAAGAA
                     LGSAVPVIGTAVGAVIGGTLALIFG"
                     /name="hypothetical protein"
     gene            2080..2379
                     /locus_tag="KI741_RS28185"
                     /old_locus_tag="KI741_28190"
     CDS             2080..2379
                     /locus_tag="KI741_RS28185"
                     /old_locus_tag="KI741_28190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023281092.1"
                     /translation="MATATNFDAWLDDVDGDYEEVMALYDSVQNVSDMGLYQCVEGGRG
                     DAWVVSSNHHPEALFLASAVARDTFLKLIRERLCGGEDVDSWYGFQRNISNDHS"
                     /name="hypothetical protein"
     gene            2450..3208
                     /locus_tag="KI741_RS28190"
                     /old_locus_tag="KI741_28195"
     CDS             2450..3208
                     /locus_tag="KI741_RS28190"
                     /old_locus_tag="KI741_28195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_214018514.1"
                     /translation="MALMVTRRYLLGGEYPSEEFVQASLEKYFFRQGFDIDTGSYIDLI
                     CRDKESRDVVWHIEVKGKTSQPGLDFRTCLGQLVQRMTKDNINYAIAVPRIKQYERLIE
                     ETSILAVSKLNLHWIFVGPDGSVTIKEPDSSTMKNGNGNGNGNGNGNGNGNGREVSLVN
                     GGFKRFIPGDFMPACSYGKAPVVFKSSRREMWDWLDDFVISNGVIPSIADIKEEAESRG
                     GKWTLSNVKIEYQFWKIYHLYKGHFFYQSE"
                     /name="hypothetical protein"
     Typing          complement(72..457)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.023"
                     /coverage="89.0"
                     /evalue="3.81e-158"
ORIGIN
        1 gagacatgtc accaaatctg tcctttcagt gtagccgcag tcaggccacc acttcaagaa
       61 ctctcgatac atctctcgca catcctgtct accagtggcc gttgtcaatg gcgattagtc
      121 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      181 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      241 ccaacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      301 tctggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcgaccag ggggaaacgc
      361 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agattctgtg
      421 atgcttgtca ggggggcgga gcctatggaa aaacgccccg ccggggcgtg gccttttcgc
      481 ccttgtcctt agctttcgtt atgcttaccg ttacgttatc tgcttatctt ccctccgctc
      541 gccgcagact catgaccgaa cggagtgagc gaatgagcga ggaagcggaa aaccatcaga
      601 acctacagta agcactttcg cgccggggac ttcatgaaca aacaacaaaa gagagccgtt
      661 aacatggcta aattcatcca ggatcagtcc ctgcttcttc tggacaggct taatgagctg
      721 gatctggatc atgaggctga tttctgcgag aaactccatg aggatgcgga acgcctttac
      781 cgaagcctgt cagcgcgcct gaatgagcag caggacggtg cgtaatggct ctgttctggg
      841 tattaccgga gtaataccgg catcgcctta attttcattg tcctggcgct taacattatt
      901 actccggtaa tacccagaat ccctttattt tttctttttt atttattttt catgaagttg
      961 cgatgaaaat cgcggctgcg ttaggtgtat gtcatttttt agtatataaa acgaccactc
     1021 ccgagtgctg gcgccgcagg ttaaggatga ttctggctaa aaagcgtcct tttccgggaa
     1081 agggtcatgc tcgtatgacc ctatgggttc agcgactaca cataaactac aaaattgcat
     1141 ctgcttttac cccagaacgc gggtgtacaa cagaattaca tcaaaagtac atcactttta
     1201 catcattttt acgtcagatc tgacctctga gctattcgta catccgcccc ctcttcctgg
     1261 gcctcctgca cgtgctgaaa atggtttttc cggctgtgcc ggcaacgcct cacggccacg
     1321 caataaattg cgtccccgtt caccgttttc gggtaagaag aacatcggaa acaggactta
     1381 ccccggctga atggcgtgaa tttccgcgct atgcacttgt gcgcatactc atgcatgccg
     1441 taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc tgttttaccg
     1501 gtttcccgtc agaaacgcgc tcaggccgtc tgagcggtgc gcgtaatgag gcgttatggt
     1561 aaatgcaggg tttttaatat atgtagttga aatgtagcct aggtggaggg agagtgtttt
     1621 tggtgtatac gtttcaatct tacgtgaggt gcgaatgagc aatattaaac ctgatagtct
     1681 gatccggatt acaaaaagaa agaatgaaaa tggcgatgaa acaacagatt tagaatggaa
     1741 gcctggttca aaagaggcaa ttgccacagg cgcggctgct ggagcagcac taggttctgc
     1801 tgttcctgta ataggaactg cagtcggggc tgtgatcggt gggactttag ctttaatttt
     1861 tggataacca agtttagagc gaaacacccg tcaagaatgg cggaaggtag cctgctaaaa
     1921 gcgggctttt ttacgctcat gcgagcaatg aggcgttatg gtaaatgcct gtcttgctca
     1981 cttttagagc atgaggcaaa aaaggtaaaa acttatttgc ttaacagccc ctatcactat
     2041 gtaaattaac tcctgaaaac taaaaaatgg aggattggca tggcgactgc tactaatttt
     2101 gatgcgtggt tagatgatgt tgatggcgat tatgaggagg ttatggctct ttatgattcc
     2161 gttcagaatg taagtgatat ggggttgtat cagtgtgttg agggtggtcg gggtgatgct
     2221 tgggtcgtga gctctaatca tcatccagaa gctctttttc tagcatcagc tgtggctagg
     2281 gatactttcc tcaaattgat tagagaaagg ctatgtggcg gcgaagatgt tgattcatgg
     2341 tatggatttc aacgaaatat atcgaatgat cactcgtaat cgtacagaga gttccctttg
     2401 taggaactct taaaatatga cgttatagta aataggatgc gtaggggttg tggctcttat
     2461 ggtgacgaga aggtacttac taggtggtga atatccttcc gaggaatttg ttcaagcttc
     2521 tttagaaaaa tatttttttc gacaagggtt cgatatagat acgggatctt atattgacct
     2581 tatttgtcgt gataaagaat ctagggatgt tgtgtggcat attgaagtaa aagggaaaac
     2641 atcacagcca gggttggatt ttcgaacttg tcttggacaa ttagtgcaga gaatgacaaa
     2701 agataatatt aattatgcta ttgctgttcc aagaataaaa cagtatgaaa gattaataga
     2761 agaaacgagc attcttgctg ttagtaaatt gaatttacac tggatatttg ttgggcctga
     2821 tggcagtgtg acaattaagg aacctgatag ctctacgatg aaaaatggta atggtaatgg
     2881 taatggtaat ggtaatggta atggtaatgg taatggaaga gaagtttctc ttgtgaatgg
     2941 agggtttaaa agatttattc cgggggattt tatgccagct tgctcatatg ggaaagctcc
     3001 tgttgtattt aaatcatctc ggcgagaaat gtgggattgg ttggacgatt ttgttatttc
     3061 aaatggggtg atcccaagta ttgctgatat taaagaagag gctgaatcgc ggggtgggaa
     3121 atggacgctt tctaatgtta aaatagagta tcagttttgg aaaatatatc acttgtataa
     3181 ggggcatttt ttctatcaat ctgagtagct gactcatttg ttcatcaaat gccatgatct
     3241 tcttgagatt cattcgctca aagcgtaatc tctttctcgg aaaacgaaaa aaccacctgg
     3301 ggaggtggtt tttcgaaggt tcagtaagtt ggggaacttc tgaaccgtgg taacagtctt
     3361 gtgc
//
