LOCUS       NZ_CP074706             8909 bp    DNA     circular CON 25-AUG-2023
DEFINITION  Escherichia coli strain 702/18 plasmid p702_18_5, complete sequence.
ACCESSION   NZ_CP074706
VERSION     NZ_CP074706.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19105266
            Assembly: GCF_019086825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Schuster,C.F., Weber,R.E., Weig,M., Werner,G. and Pfeifer,Y.
  TITLE     E. coli harboring novel ESBL variant blaCTX-M-243
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Schuster,C.F., Weber,R.E., Weig,M., Werner,G. and Pfeifer,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2021) Unit 13: Nosocomial Pathogens and Antibiotic
            Resistances, Robert Koch Institute, Burgstr. 37, Wernigerode 38855,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Assembly Name                     :: RKI_Ecoli_702-18
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 163.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/25/2023 04:43:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,460
            CDSs (total)                      :: 5,337
            Genes (coding)                    :: 5,093
            CDSs (with protein)               :: 5,093
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 102 of 244
            Pseudo Genes (incomplete)         :: 163 of 244
            Pseudo Genes (internal stop)      :: 50 of 244
            Pseudo Genes (multiple problems)  :: 58 of 244
            Pseudo Genes (short protein)      :: 4 of 244
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074706.1.
            Bacteria and source DNA available from Robert Koch Institute, FG13,
            Nosocomial Pathogens and Antibiotic Resistances, Burgstr. 37,
            Wernigerode, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="702/18"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p702_18_5"
                     /geo_loc_name="Germany"
                     /collection_date="2018"
                     /genotype="ST354"
     gene            complement(<1712..1888)
                     /locus_tag="KH969_RS26970"
                     /old_locus_tag="KH969_26980"
                     /pseudo=""
     CDS             complement(<1712..1888)
                     /locus_tag="KH969_RS26970"
                     /old_locus_tag="KH969_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(2414..2833)
                     /locus_tag="KH969_RS26975"
                     /old_locus_tag="KH969_26985"
     CDS             complement(2414..2833)
                     /locus_tag="KH969_RS26975"
                     /old_locus_tag="KH969_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2917..3336)
                     /locus_tag="KH969_RS26980"
                     /old_locus_tag="KH969_26990"
     CDS             complement(2917..3336)
                     /locus_tag="KH969_RS26980"
                     /old_locus_tag="KH969_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312320.1"
                     /translation="MAWHTLQNALKMTGRSRSQFYRDMRAGLVSYRTGQDGRREFETSE
                     LIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATERR
                     RQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            3614..5179
                     /locus_tag="KH969_RS26985"
                     /old_locus_tag="KH969_26995"
     CDS             3614..5179
                     /locus_tag="KH969_RS26985"
                     /old_locus_tag="KH969_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447001.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEAEEKRLAALNEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRLSSSI
                     HARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRRTGAGK
                     IMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKTVDAGVSYIVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTIND
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(5176..5517)
                     /locus_tag="KH969_RS26990"
                     /old_locus_tag="KH969_27000"
     CDS             complement(5176..5517)
                     /locus_tag="KH969_RS26990"
                     /old_locus_tag="KH969_27000"
                     /inference="COORDINATES: protein motif:HMM:NF015489.3"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058758.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            5565..5702
                     /locus_tag="KH969_RS26995"
                     /old_locus_tag="KH969_27005"
     CDS             5565..5702
                     /locus_tag="KH969_RS26995"
                     /old_locus_tag="KH969_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001519492.1"
                     /translation="MRKRFFVGILAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(5899..6057)
                     /locus_tag="KH969_RS27000"
                     /old_locus_tag="KH969_27010"
     CDS             complement(5899..6057)
                     /locus_tag="KH969_RS27000"
                     /old_locus_tag="KH969_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354639.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAAVGNLVSRAMMA
                     QMVQQSS"
                     /name="hypothetical protein"
     gene            complement(6727..6918)
                     /locus_tag="KH969_RS27005"
                     /old_locus_tag="KH969_27015"
     CDS             complement(6727..6918)
                     /locus_tag="KH969_RS27005"
                     /old_locus_tag="KH969_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6982..7305
                     /locus_tag="KH969_RS27010"
                     /old_locus_tag="KH969_27020"
     CDS             6982..7305
                     /locus_tag="KH969_RS27010"
                     /old_locus_tag="KH969_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955996.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7514..>7945
                     /locus_tag="KH969_RS27445"
                     /pseudo=""
     CDS             7514..>7945
                     /locus_tag="KH969_RS27445"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            7983..8501
                     /locus_tag="KH969_RS27020"
                     /old_locus_tag="KH969_27030"
     CDS             7983..8501
                     /locus_tag="KH969_RS27020"
                     /old_locus_tag="KH969_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_029400393.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            8508..8741
                     /locus_tag="KH969_RS27025"
                     /old_locus_tag="KH969_27035"
     CDS             8508..8741
                     /locus_tag="KH969_RS27025"
                     /old_locus_tag="KH969_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029400396.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          6561..6642
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(5986..6532)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.841"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          7296..8845
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.893"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcagggcac taggaactag cttcagtcag gccttgctga aaacacagga acgcgttaaa
       61 acgcatacgg acgcgtttct ggcgctcaaa aaccatgggg ttagtgtggg tagtgtgtat
      121 agcaacagga gcctggaacg cgttccgctt cttctgtaat cgacgccaga atgcgtttaa
      181 tgcttcttct gatggtatca gagaggagct gcttgagtta ttcctgttgg ccacagagcg
      241 taattatctc cttttcagat ccacacgggg atacgtcaga atcggaactg agcaaggatt
      301 actgaaagat actgtttgcc agaaaaaaaa tcctcataca tgatgaggat aaagtcttaa
      361 aggtccattc tatggagtgt cggattgaaa aatttctccg gatgatatcc ttcacagttt
      421 aaatgtgatt tcaaccataa gttttttatg gattttgtta aataaaaaag aattgagtcg
      481 tgtttctgat atgtctgaat ataataagtg aacagttata aaataattgt tttatattta
      541 ttgtgagttt gatgttatgt tctgcataaa taactgtagt ttagaatatt attcttttat
      601 atttatagct gtgagatgat aaattataag tgttctttgt gctataaaga accccccgta
      661 taaggggggg taaaagatgg gtttacttct ttttagtcca tgccctggtg agctacatga
      721 agctcgacac tacagtaaaa atttgcagtt tttttgaact ttacgcaagc aatttattta
      781 cattgtaaag aaaaccccct caattccgga gggggagtgt agggaagtgc ctgatttatt
      841 ttatgtacgc gcaaaaatga gatagctttt attagatatc atcagccctg tctgcatcag
      901 tcagctcctt acagcgtcag gagtactgat gaagggggga acgttcctgc gtttttgcaa
      961 aggcaatccg gctagaggct ttactacgca gaaacattcc ggacaacttt ttaccccagt
     1021 gtaacttgta gatcaaccag attttgttgc agtaaaaata cagtggtttc gaatttgtaa
     1081 catttgtcac aaaaataaca tcaatatgtt ggttttctgt tgataaatgg tttggtcgct
     1141 gattatgatt tccttgtact ccacaggagt acagtcatgt gcatactaaa atccgtgttt
     1201 tcgataaaat taaggccgtg atacagaatc acggcttttt tcttgcctga aaggatcatt
     1261 tacgctagaa gaattatgag cgcgtctctg gtgtttgttg cagggataat atctaatatg
     1321 gggcaccgga aacgcgctct cccttcgtat acagaatgcc cattaatttc ttcttcctga
     1381 ttttccagac atatattctt gtgtaaatta aaaaacagag cgtgtttctg gtgtgaattt
     1441 gttggctaca atgtgcattg ttgttctgtg ttccagaaac acgcttcttt tttgagatgc
     1501 acatcggtct tatacaaaga gggatggttg tatctgtgtc agagttttgt tattttttgt
     1561 atgctcagtt attgatggtt aataatatca agaaaatcag caagttcctt acactacccc
     1621 tcttcagtag aggggttttt ttatctggta cgccgtaaaa cccggtcctt cagggcgtag
     1681 aggatgtcaa aaatttcttc gaaaggtaat acccggtatc ccgctcatgc tggcgaaccc
     1741 agacacgcag ggtctctggc gtacagccaa tctttggagc aatggaacaa attgccgccc
     1801 attgtgagtc atattcgccc tggctttcca gaaccatacg aactgcccgt tgacggactt
     1861 cgggggaaaa acgagtattt ttagtcatcc tgtttacatc tttctcagga agtttagtct
     1921 ccaggatttc cggggcggtt cagtccggca gagataaatt gccacgggaa gttagagaaa
     1981 agtgcgggaa aatgtcttaa gaatgattct ggaagaacga aaagtaagaa gcgcaaaggc
     2041 tatgagggca aaagagtatc gtgccgcagc gaatactttt acctggcagc ctgtacagcg
     2101 caggtgaata gacattcccc tgactcatcc tgctcccctt ggcggcgaag gggattaatc
     2161 cctatcctgt aacttctatt ttgtggaatc gaaaacagct gaaaattgtg aaaaaaaacc
     2221 gtttcagtcg tctcagtaag gaaattcgct tgtgattcac ctggatgcat aggtatgcat
     2281 aaggttaaaa tttatcaggc gcgatcgcgg tagtttttcg ggggggttgt tgcgattttt
     2341 acctgtctgc tgccgtgatt gcgctgaatg catttcagcg gtgcgtacaa ttaagggatt
     2401 atggtaaatc cacttactgt cttccctcgt agccatcgag ataaaccgca gaaatcgtct
     2461 cagccagcag cctggatttt ttcggggtaa ggtctttgct gcggtaatca gtacccgcaa
     2521 attgagttgt gccccggagt gagtttaaaa attcctggct gtgaaactgc tgaatgcgtt
     2581 gctgcacatc actccgtgat tttcctgtcg ctttatcctt tgctcccata tcccagaact
     2641 gccggaaaaa aggcaccatc attcgtgttg tgtcggcctt ctgggtggaa aagttaggat
     2701 caaaaccatc gtctgatgca taaacgtaat gccgggtatg ttgttccaca ctgccctttg
     2761 gcgtgcaggc tgtcaggacc gttgccgctg tcagcagggc gataagatac agtgtgccgc
     2821 cggaacgtct catgagtgtc tccttacctt taaatctttg tactcagtga cttatattta
     2881 accgctgact atcttatcat cacttgcagt cggtgctcac cggctgaaca gtctggccca
     2941 gaatccccgt tttttttcct gctccagtgc ctgcctgagc tgctctatct ctttatgtag
     3001 ctgttcccgt tcggcttcct ggcgccggcg ttccgtagcc tgtgcctgtt tgtcctcaag
     3061 catcagtgtc aggcattgtc tcagctcatt cagctcccgg agaatgcgtt ctgtctgctg
     3121 atcatgtgga ttttctgcct gtcccgcact gtgcctttct ggtgtctcat cctgctttaa
     3181 ttcgccgtat gcccggatca gttcactggt ttcaaactcc cgccgtccgt cctggcctgt
     3241 acgataactg acaaggcctg cgcgcatatc acggtaaaac tgtgaacgcg atctgcctgt
     3301 cattttcagt gcgttctgga gtgtgtgcca tgccatgaag tgacagtgtc ccattaatgt
     3361 ctcatatcat ggtttcagta aaacatactg gggccggaga acggtaatgt ttgtgctggt
     3421 ttttgtggca tcgggtggga atagcacgtg gtgtgggtgg ctgttttttt gatcgttttc
     3481 acaaaaatga aaatccacag ggttgacagg gaaaatgcag cggcgtaatt ttaagctgta
     3541 tataaaacca gtggttatat atacagtata ttgcttttaa ctatttgttt ttaatgtcaa
     3601 agaggatttt ataatggaaa cagctgtagc gtactataaa gatggtgttc cttatgatga
     3661 taaggggcag gtaatcatta ctcttttgaa tggtaatcca gacgggagtg gctctggcgg
     3721 cggtggtgga actggaggta gcaaaagtga aagttctgca gccattcatg ccacagctaa
     3781 atggtctact gctcaattga agaaaacgca ggcagaacag gctgcccggg caaaagctgc
     3841 cgcagaagca caggctaaag caaaagcaaa ccgggatgcg ctgactcaac atctgaagga
     3901 tattgtgaat gaggcgcttc gtcataattc cactcatccg gaggttattg acctggctca
     3961 tgccaataat gcagcgatgc aggcagaagc agagcggttg cgccttgcaa aagcagaaga
     4021 aaaagcccgt aaagaagcgg aagctgcgga aaaggctttt caggaggcag aacaacgacg
     4081 taaagagata gagaaggagc aggctgaaac agaacgccag ttgaaactgg ctgaagctga
     4141 agagaaacgg ctggcagcat tgaatgaaga ggcccgggca gtggaggtgg cacaaaaaaa
     4201 tcttgctgct gcacaatctg agctggcgaa agtggatgaa gagattaaga ctctcaatac
     4261 ccgtttaagc tccagtattc atgcccgtga tgcagaaatg aatacgctgt ccggaaaacg
     4321 aaatgagctg gctcaggcat ctgctaaata taaggaactt gatgaactgg tcaaaaaatt
     4381 gtcaccaaga gctaatgatc cgcttcagag tcgtcctttt ttctatgcca ccagtcgacg
     4441 gacgggagcc ggtaagatta tggaggaaaa acaaaaacag gtaacagcat cagaaacgcg
     4501 tattaaccaa cttaatgctg agataaatgg aattcagggg gctatgtctc aggctaataa
     4561 taatcgtaat acagctgttt tacgtgttca tgaatctgaa gaaaatttga aaacagcgca
     4621 gactaatctc ctgaactcgc agattaagga tgctgtggat gcaacagtta gcttttatca
     4681 aacgctatct gaaaaatatg gtgaaaaata ttcaaaaatg gcacaggaac ttgctgataa
     4741 gtctaaaggt aagaaaatca gcaatgtgaa tgaagctcta gctgcttttg aaaaatacaa
     4801 ggatgtttta aataagaaat tcagcaaagc agaccgtgat gcgattttca atgcactgga
     4861 atcggttaag tatgaagact gggctaagca tttagatcag tttgctaagt acttgaagat
     4921 tacgggacat gtttcttttg gatatgatgt ggtatctgat atcctaaaaa ttaaggatac
     4981 aggtgactgg aagccactat ttcttacatt agagaagaaa actgtagatg ctggagttag
     5041 ttatattgtt gttttacttt ttagtgtgct tgctggaact acattaggta tctgggggat
     5101 tgctattgtt acaggcattc tatgtgcctt tattgacaag aataaactta atactataaa
     5161 tgatgtgttg ggtatttaat tattctttat tgatataaac agtcctagca atccaaatgg
     5221 gattgctagg accaaacaaa gtagattgta tagcataaat aggtttaatt ttgccacggg
     5281 ggcattattt agattttttc tttttctgaa aaaatctttc tttatgaact taaaaaatat
     5341 gtgttcaata gcatattttg aatatgggca tagaatagtg cttatcacga ttgcgtatag
     5401 catcttatct gacgctagga aataataccc ttcgttgttt tttgttataa ggtatatata
     5461 tataagtgtg caatataggc caaacaaaat gttttttatg tagtatctta aactcataaa
     5521 ttaaacctcg ttatatattc ttttcatttt ataaggatcg agttatgagg aaaagatttt
     5581 ttgtgggaat attagctata aacctccttg ttggatgtca ggctaactat atacgtgatg
     5641 ttcagggagg gactgtggct ccatcatctt cttctaaact gacggggatc tctgttcagt
     5701 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcctgta
     5761 aacgaaaaaa ccgcctgggg aggcggtttg atcgaaggtt aagtcagttg gggaactgct
     5821 taaccgaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ccagtgtagc
     5881 cgtagtcggg ccactacttc aagaactctg ttgcaccatt tgcgccatca tcgctctgct
     5941 aaccaagtta ccaacggctg ctgccagtgg cgctttgtcg tgtcttaccg ggttggactc
     6001 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6061 gcccagcttg gagcgaacga cctacaccga actgagatac caacagcgtg agctatgaga
     6121 aagcgccacg cttcccgaag ggagaaaggc ggagaggtat ccggtaagcg gcagggtcgg
     6181 aacaggagag cgcacgaggg agcttccggg gggaaacgcc tggtatcttt aagtcctgtc
     6241 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6301 ctatggaaaa agcgcctccg gcgcggcctt ttctggggtc ttcgtttttt gctcacatgt
     6361 tctttccggc gttatcccct gattgtgtgg ataacagtat caccgctttt gagtgagcag
     6421 acaccgctcg ccacagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6481 agcatcggca tctgcatgtt ctccttacgc atctgtgcgg catttcacac cgtacatggt
     6541 gcacttttca tacaatccgc accgatgccg catgactaag ccagtataca ctccgctatc
     6601 gctacgtgac tgggtcaggg ctgcgccccg acacccccta aaacctgctg acgcgccctg
     6661 acgggcttgt ctgctcccgg catccgctca cagacaagct gtgaccgtct ccgggagctg
     6721 catgtgtcag aggttttcac cgtcatcccc gaaacgtgcg aggcagctgc ggtaaagctc
     6781 atcggcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagttcgtt
     6841 gagcttttcc agcagcgtta atgtctggct tctgataaag cgggccatgt taagggctgt
     6901 tttttcctgt ttagtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg
     6961 ataccgatga aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgccagg
     7021 ttacttgaac gctgtgaggg taaacaactg gcggtatgga tgcggcgggt atgcctgggt
     7081 gagccggttg cccgttccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
     7141 gctgccatcg ggaataacct gaatcagacg gcgaggaagg tgaacagcgg gcagtggtct
     7201 tccggtgacc gggttcaggt ggtggccgca ctgatggcca tcgagagaga gttgcgcagc
     7261 ttacggcagg tcgtcaggga acagggagcg cgggatgata gttaaatttc atgccagggg
     7321 aaaaggcggt ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg
     7381 ggcaacggtg cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt
     7441 tgcgaagaaa tacacgtcag gtgttctgtc gttcgcggag aaggagctgc cgccgggagg
     7501 acgtgaaaaa gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca
     7561 gtacagcatc ctgtgggttg agcaccagga caagggacgg cttgagctga attttgtcat
     7621 tccgaacatg gagctacaga gcggaaaacg cctccagccg tactatgacc gcgcagacag
     7681 gccgagaatt gatgcctggc agacgctggt gaatcaccat tacgggctgc atgacccgaa
     7741 tgccccggag aaccgcagga cgctgacact ccctgataac ctgccggaaa cgaaacaggc
     7801 gattgctgag agcgttacac gggggcttga tgtgctttac cgtgccggag agataaaaga
     7861 ccgtcaggat gtgattcagg cactcacaga tgtgggagtt gaggttgtca gggtgacgcg
     7921 aagcagtatc agcattgcag acccgaacgg cgggaagaat atcaggctga aaggagcgtt
     7981 ttatgagcaa tcttttacag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg
     8041 aatctacaga gaaagtgctg aacaacgagt tcaggaggct cggcgaatct gtaagcgagg
     8101 ctgtgacatc aaacgagacg aaaatcagag acgctatagc cctgttcaca gcctcgacag
     8161 aggaatcgct ggagaaacac cgggaagggg tgaaagaggc gatgaggcag cacaggaagg
     8221 acgtgttaaa gctggcaggg aatacgggca tgatgttact ggggatagtc tttctcctgt
     8281 ttaccgcgag tggcgggacg ctctggtatc ttggcgggag gatacaggcg aacctggaag
     8341 atatcaggaa gcaggaagag acattgcaga aactgaacgc gaagacatgg ggcgtggagt
     8401 tcgtgcagga cgggaacagg aaatttctgg tccttccgta cgggaaatca gcagaggtga
     8461 ttccctttca ggggaaagag tgggtacatc tgaaggagtg acacagagtg acagagctgg
     8521 aaacacattt gctgaacgcc ttagagcagc tgcaacagga ctatatgcag cggctgaacg
     8581 aatgggagag cgccttcgtg gaattgcaga agatgttttc gcttacgcaa cgggacaacg
     8641 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     8701 cagaacactt gagccagtta tacagcgaga gcaggagata agggacgaac tactgataca
     8761 ggaacgtgaa catgagctat tcctagagcg tgagcgccat ctggaaatac aggaacggac
     8821 actggatggc ccgtcgctgg gatggtaacc aaagacaggc taaagtgttg tcagcgcgtt
     8881 tccggtgcat ttttttggat aatggttgg
//
