LOCUS       NZ_CP074696             8961 bp    DNA     circular CON 20-APR-2024
DEFINITION  Acinetobacter baumannii strain X4-107 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP074696
VERSION     NZ_CP074696.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16560992
            Assembly: GCF_029774295.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Hsieh,Y.C., Wu,J.W., Chen,Y.Y., Quyen,T.L.T., Liao,W.C., Li,S.W.,
            Chen,Y.C. and Pan,Y.J.
  TITLE     An Outbreak of tet(X6)-Carrying Tigecycline-Resistant Acinetobacter
            baumannii Isolates with a New Capsular Type at a Hospital in Taiwan
  JOURNAL   Antibiotics (Basel) 10 (10), 1239 (2021)
   PUBMED   34680819
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   Pan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2021) Department of Microbiology and Immunology,
            China Medical University, Taichung, Taiwan, No.91 Hsueh-Shih Road,
            Taichung 40402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye (v2.7) v. Flye (v2.7)
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 306x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029774295.1-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 06:38:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,862
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 46 of 91
            Pseudo Genes (internal stop)      :: 19 of 91
            Pseudo Genes (multiple problems)  :: 12 of 91
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074696.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="X4-107"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed1"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24.99290100 N 121.30097200 E"
                     /collection_date="2020"
                     /collected_by="Chang Gung Memorial Hospital"
     gene            complement(262..516)
                     /locus_tag="IS508_RS19860"
     CDS             complement(262..516)
                     /locus_tag="IS508_RS19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     gene            1200..2150
                     /gene="repM"
                     /locus_tag="IS508_RS19175"
     CDS             1200..2150
                     /gene="repM"
                     /locus_tag="IS508_RS19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            2143..2718
                     /locus_tag="IS508_RS19180"
     CDS             2143..2718
                     /locus_tag="IS508_RS19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            2738..2881
                     /locus_tag="IS508_RS19185"
     CDS             2738..2881
                     /locus_tag="IS508_RS19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(3064..3891)
                     /locus_tag="IS508_RS19190"
     CDS             complement(3064..3891)
                     /locus_tag="IS508_RS19190"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            4200..4487
                     /locus_tag="IS508_RS19195"
     CDS             4200..4487
                     /locus_tag="IS508_RS19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            4474..4788
                     /locus_tag="IS508_RS19200"
     CDS             4474..4788
                     /locus_tag="IS508_RS19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            4916..7327
                     /gene="znuD2"
                     /locus_tag="IS508_RS19205"
     CDS             4916..7327
                     /gene="znuD2"
                     /locus_tag="IS508_RS19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            7632..8093
                     /locus_tag="IS508_RS19210"
     CDS             7632..8093
                     /locus_tag="IS508_RS19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(8416..8589)
                     /locus_tag="IS508_RS19215"
     CDS             complement(8416..8589)
                     /locus_tag="IS508_RS19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(8589..8960)
                     /locus_tag="IS508_RS19220"
     CDS             complement(8589..8960)
                     /locus_tag="IS508_RS19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     ARG             complement(3068..3891)
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1201..2150
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcttattaaa attagatttt gtacaaaatt aaatatatat aattcaacta attaaataaa
       61 aaataataat tagattacgt acaaacttat ttgtacagaa ttaatttatt ctaaaacaaa
      121 aacttattta aatataatgc tttttttatg tacaatcatg agtacatatt ttttcttaaa
      181 tttatcaata tcttatctaa aaaaagtgtt gttttgatgt acaaccatga gcacagcata
      241 aaaaatagat aattcaatca cttattctaa aaaaagacca tttttctgta cacgtttctg
      301 agaccctaaa aaatcgctga aatccttatc ctgtaaggct ttgaggggtg tcatcgttag
      361 atgcgtggtg tgtgacaaaa aaatcccggc atgtgccgga tcttggattg gaaaattggc
      421 taaagtgacg tagggccggt gcttggtttt gcatggaaaa aagtatttat ttccttgttt
      481 agaaaaacgt aaaaaatcaa ttttttgtcc attcatccag gttaaaaatt tcaacccaaa
      541 actttaatta tgaaaagctt catagaacgc gttcaaatgc gatttaagag cctttaccta
      601 gaaaacatag atctcatagt gaaaaacaca aaacagctca aaaaacgcaa atgagagcga
      661 aataaagagg tattttgact ttagatagat gcatagagcg agtgtctacg agcgaactat
      721 caaaatttgc gcctagaccc tctgaaaaac atttttttgt cctctttagc ctaagaaagc
      781 gtaagtttcg tgcagaaatt tgctcctgga tcgagcgtag cgagtaaaaa agttttatga
      841 gcgaagcgaa ttccaagttt cttttgcttt tttttaaagt cacgccagta ttaaccaaaa
      901 aattgcccca acgaactgag cgaaagcgaa gttcaataga gtttgagcga agcgaaaacc
      961 aagggcaatt tttcattccc tgggctttta attattttaa agtttttaaa tacttttaaa
     1021 tggcttgaaa gccttatgaa taaagggttt tagctcttat atgtccacgt ttaccttgca
     1081 atatgtccac gtttaccttg caatatgtcc acgtttacct tgcaatatgt ccacgtttac
     1141 cttgcattag tacacaaata atattaacgt gtacttatac acaataaaaa atagtggcta
     1201 tgagagattt agttgtaaag gacaatgcct taatcaacgc aagctataac ttagacttag
     1261 tagaacaacg tttaatttta ttggctattg ttgaagcaag ggaaagtggg aaagggatta
     1321 atgcaaatga tccattagaa gttcatgcag atagttatat caatcaattt ggtgtacacc
     1381 gtaatacagc ttatcaagcc ttaaaagatg cttgtaagga tttattcgcg cgtcaattta
     1441 gctatcaaga gaaaaaagct aatgggaata tccgaaatgt tatgagtcgt tgggtatctc
     1501 aaattgctta taacgacaat gaagcaactg tagacttaat atttgcacct gctgttgttc
     1561 ccttcataac ccgactggaa gaacaattta ctaaatatga attacagcaa gttagtagtc
     1621 ttagtagcgc ttatgccatt cgcttatatg agcttctaat tcagtggcga agcactggta
     1681 aaaccccaac catagaacta caagaattta gaaagaagtt aggcgttctt gataatgaat
     1741 atttacggat ggctcattta aaagagcgtg ttttagagct ttcaattaaa caaataaatg
     1801 agcatacgga tataactgta aaatatgaac agcataaaag aggacgttct atttcaggat
     1861 tttcttttac ctttaaacag aagaagaagg atagcccatc aatagaaaga gatccgaaca
     1921 ctttggagct tttttcaaag atgaccgatg ctcaacggca tatgtttgca aataaacttt
     1981 cagaactccc tgaaatgggt cgctattcac aaggaacaga aagctaccaa cagtttgctg
     2041 tacgtattgc tgagatgcta caagatcccg ctcaattcaa agagctatac ccatacctaa
     2101 aaaaagtggg atacatgcca tcaaataaaa aggacaccgt aaatggctaa gttatcacta
     2161 agtgaagtat ctaaaaaatt tcatgtggat agatcaacca tttacagagc tgtacgtaat
     2221 ggacgtttat cacgctccag tgatggacaa ttcgatctag cagaggtcat acgatgcttt
     2281 ggagaacctg agcaaacatc tcaaaaaatt gaatcatcta agcaagaagg tgatgaatct
     2341 acaaaaaaac ttattgccca tttagaaaat gaagtcaaaa aataccaaga acgtgaagaa
     2401 cggttaatgc aacaaattga ccgtatgcaa acactcattg agctgaaaag tgttgcacct
     2461 gccacagcag caccacacca agatgctacg gcatgcgaca ccaagatgcc acagcatgcg
     2521 acaacacaac aagacactga taacaaaaag aataatgaat taaatattgc agaaaatgta
     2581 gcagtgccac agcaagaaac tacggcatac cacacccaaa cgctacagca tgccacgttg
     2641 caaagtgtgg cagtgccaca acacaaaaaa cgtggtttat ttggccgtgt gctgaatgcc
     2701 gtctttgata atgactgagc aataagggtg aaagatcatg ccgaaactga aagacattgc
     2761 cctgggaatt attgtagctc cgctgctgat cccgatcatg ctgattgcat cgtaccagga
     2821 taaaaaggcg ctgaagaaag agctggatga atgccaaaaa gaaaaagacc aggcatcctg
     2881 attattcaat tgagctaatc aaaagtgtgg aacattgatg aaataatagc gtggaacgtg
     2941 aaaagtcata aaaaaagccc gaacagggat gctcgggcta taaactagaa actacagcgt
     3001 tgaattataa ggataattat acagcatttc gtataaggcg tattatgtta attttagaaa
     3061 ttattaaatg attccaagat tttctagcga cttataagta atttcattac gaatagaacc
     3121 agacattcct tctttcattt ctaagttgag cgaaaagggg attttttttc cattagcttg
     3181 ctccacccaa ccagtcaacc aacctacctg tggagtaaca tccattcccc atccactttt
     3241 tgcataaatc ttactaccat ttacttcttt aattagaagc atttttttaa cttcttcttg
     3301 agtttctaat ttaaaaggta atcggttatg tgcaaggtca tcggcaaaat taacttcttg
     3361 tactggtgta attttaaggg ggccaactaa ccaaaaatta tcgacctgtg ttccaatatt
     3421 tgtatttcca aaattaaccc gctttacttc tttctgcatt agctctaggc cagtccgtct
     3481 tgcaagctct tgatatactg gaactgctga caatgccatt gcctcaccta aagtcatatc
     3541 tttctcccac ataggataag ttcttttttt accatcccat ttgaaaatct catttgttgt
     3601 tgctttatga ttttctagcc cgattaaagc atttagcatc ttaaatgttg atgcagggac
     3661 atattcttta tttgctcgtg caagagcatt accataggtg ctaagatttt taccctcttt
     3721 aataataatt acaccctgtg tttgagcttc atcaaaatag cttttaatag ctttttcatg
     3781 ttgctgagaa gaaatatgaa aattatcttc agatttagtt ttaatagatg aacatgcact
     3841 gagagaaact agaatagaaa tgctgaatat aggaagtata aattttttca ttacaaattc
     3901 atgttagggg aaattttggg gcttagagta taatattttt attaaaaaag ttaaaactct
     3961 tttaatttaa cataatggac gttatgcgaa gtacacttgt atattaatat aaatatcaac
     4021 tacttaataa aaatagaaaa atcccaaaat gcccaaaaag ccgactttga aacaagtcgg
     4081 cttttttatg agcagttttg atacttcttg ccaataattt tgactaaaaa ttacacgaat
     4141 ctggttgagt gatacaattt tgtgatacat tattgtatat acaaatttga atgtttttta
     4201 tggaacagta ttttgaatgg gatgaggcta agaatcgaaa gaatcagaaa aagcatgata
     4261 tctctttcga aactgcaagc cttgtttttg aagatccatt acggatctca atccaagaca
     4321 gacataccaa tggtgaagaa cgttggcaaa ccattggacg agttaaaggc gtactaatgt
     4381 tattggtggc tcacaccatt tttgatgaag atgactgtga aatcatccga atcattagtg
     4441 caaggcaagt cactaaagcg gagcgaaaca aatatgagca tggttagata ctcacacaaa
     4501 gagctgaatg agaaatttgg tgagaagcaa gatgctgaaa ttcaacgctt gcttgctaaa
     4561 ggcacggttc ctgatgatca gctagatcta tcagatattc ctgaaattac agactggagc
     4621 aatgctgtac gccaaaatca attttaccgc ccagtaaagc aacaaacttc aattcggtta
     4681 gatgctgatg tacttgcttg gttcaaagca caaggcaaag gttatcaaac acgtatgaat
     4741 gaaattttac gagatgctat gctaaaagaa ttaaaaaatc atcaataaga atgggagctt
     4801 aggctcccat ttttgacttc atttttaggg gggattacca ttcaaaaatt taaatagtag
     4861 attttataaa tttttaagtg ttatattata atataacatt aaatttggtt gttttatgct
     4921 aaataaaagt aaactatttt tagctttaat tactttaggg gcctcgaaaa ttttattggc
     4981 agctgaaggg cctgttacta ccttaaatac aatagtatta actgcacaat ctgatgagtt
     5041 agggtctgag ctgttaggca aaagtctaaa tgttagtaat cagtttattg atactagtaa
     5101 acttaaacag cgttcgacaa ctctagggga tgccttgggt acggaattag gaattcattc
     5161 taatcaatat ggaggtggag catctactcc tattattcgt gggcaggaag gaaaacgtat
     5221 taaagtttta cagaataatg ctgacgtgtt ggatatgtct aatatgtccc cagatcacgc
     5281 tgtcacagtt gaaccaagtt tagctaagtc tattgaaatt atacgtggtg caagtaccct
     5341 tttgtatagt tcaaatagtg cggcaggggt agtcaatgtc attgactata aaattccaac
     5401 ccaaatgcct caagacggat tagagggtaa tactacatta cgttttaata ctggcagtaa
     5461 tgagaagttg accacagcag gtgtaacagt tggcttaagc ccacatgtag ctttacgggc
     5521 agaagggtta tatcgtaatg ctggtaacta taaaactcct cattaccagt ctagctctta
     5581 taattcttta gaagatcttg aaaaccaaaa cagtatttat aaaaatttaa aatatttacc
     5641 agaaagctgg gcagagtcga gagtaggtac gcttggatta tcctggatag acgataatac
     5701 ttatttagga gtttcttata cacatcgtca tgatgaatat ggtttaccag cccatagcca
     5761 tttatatgaa ggttgtggtg cctctgcaat cggtatcgat tcacgaatta gtggattaaa
     5821 aaattatctt ttatattatc ctcaattgat ggatgagcaa gatattaatt acataaatcc
     5881 acgtcctgat tgtcatcagc ataatcatat tcatgaaact aatttctctc ataatgctcc
     5941 ttatattgat ttaaatactc gacgttatga tgtgcgtgga gaattcactc aaccatttac
     6001 cggtattgac aaaattcgta cctcattgag ttatatagat tattttcaca atgaactcga
     6061 gggagataag attactaact tttttaaaaa tacaggaaaa gtaggccgca tagagttatc
     6121 acatcagcca ttaggggaat taaccggtat cttggggcta caatatttgg aacaggataa
     6181 tagtgcttta tcaccagtcc attctcaaga aggccatacc acctatctag ataatcagca
     6241 gctattaaat cgaaatgtca ctaaaaactt tagtgtattt ggcctagaaa aatataactg
     6301 gaacgacttt actttcgaac taggtgctcg tatagaaaag cagaaagtta gtatggatta
     6361 tgatatagaa aaaattaaag attcaatgaa accttggcct aataaatata acagtcctta
     6421 tgtagaaaaa aataataaaa tcagggctca aaatttaaaa tctattttag aagctgttca
     6481 acctaacaaa gaaacagctt tttcttatgc aggtacagtc cactggagat ttgctccaaa
     6541 ttatatctta tcattaacag gtacacatca agaaagatta cctaatgcac aagaaatgta
     6601 tactcatggt atgcatctcg ctactaattc ttttgaaata ggtaaccgct ttctaagaaa
     6661 agagaaatcc aataatttag aaatcagtct ggcctataag gatgatttac ttgattatca
     6721 aatcagtact tactattatg attttgataa ctatatatat ttacagacac tcaatgaagt
     6781 tttaggaact acaaaagtta gagatcagca tacactacgt attaatcatt atagccagtc
     6841 tgctgctaat ttttatggat tagagggcaa catagggtat caatttaatt ctgtttatca
     6901 cggctctctt tttggagatt atgtgaaagg ccgtttaaca aatttacctg atgctgtaat
     6961 tgcatacgat atctggaata gagaaccgac tttagctccc caaaaggatc gttacactcc
     7021 tagattacct cctgctcgtt tgggtacacg actaaaagct gattttgatg aatcgcttaa
     7081 aggtgaaata gaatattatc gagtctttaa acaagataat atttctaaat ttgagcaggt
     7141 tacatcaggt tataatatgc ttaatatgac tttagcatat aaaaataagc tatctcacac
     7201 agaatatgat ttattcttta aagcgaataa tttacttgat cagaaagtct atgcccatga
     7261 aacatttttg ccatatattc cccaaattgg aagaaatttt agtttaggct taaatcttaa
     7321 tttctagata agatactgta atcttctaag aaaatttctt agaagatttt tcttataaaa
     7381 tgattagatt aataattaag gtaagcgtcc gctgaatccc atacctattt tttaattcga
     7441 tttaggtttc cagcagtgtg gcagtaactc ttcaatctgg gtcactttat gtgtcggcag
     7501 ccttttcagc actgaatagc caccgatttt gtagacacct tttagctaga taaaaagcaa
     7561 tttttttgat ctttaaagaa tgaggatata agctgtttaa aacttaattc taattggtta
     7621 ataggtgaaa aatgaaatta gcagaagctc tcttacttcg aagtgatcaa caaaaaaaaa
     7681 ttatttcact aaaacaacgt atcaatgcaa atgtattggt acaagatggt gatcaaccat
     7741 ctgaagatcc aaatgaacta ctaaaacaag tattttcttt aattcaagaa tttcaaaaat
     7801 taagttatgc aattcatgaa actaatgctt taacaaaatt aaatgatggc cgttctctac
     7861 ttgctttatt gactttgcgt gatgaatttg tagaacagca caaaacccta actgcagcaa
     7921 taagtaatac atctcgtgaa tctgatcgtt atagtacgcg ggaaataaag tggcataaag
     7981 ttattccagt ttcatcatta caaaaacaag ctgatgatat tagtcttaaa ttacgtgatt
     8041 taaacgtgtt aattcagtcg actaattgga aaattgattt gattgaagcc taatagtcct
     8101 ttagatataa tcaagttgaa catactgggc gagtacaaga tcccttactt ccacaacata
     8161 ggattgtaaa taaagtgtag tggcttgcaa agcaagtgga taagcttaaa attttaagta
     8221 caaatcaatc catcacttgg cataactcaa atctttgtgc aaacaaagtt acacgttact
     8281 tataactacc atgtactgac agtatgttca cctcaattac taaaaataaa gaaatgaaca
     8341 agcatcagct caactacatt tctattttta attcagttcg gcatttaaca taaaatctct
     8401 tatacgaagc ccaggttaat tctgggcttt ttgttcaatc tcatcattgg ccgagaacat
     8461 acttggattc gtaagcctga tattttcctg acaacttcca cacagcatca atcctgcaaa
     8521 aagttgtaca ttttgtctat tttcacaaag cgtacatggc tttgaaagct gaataatatt
     8581 atctgacact actttaccct cttatagtta tcatctgcat atgaataacc tttagcttgt
     8641 atcattctaa cctcatcttt agaagcgcca tttttcatta agagccacaa catcatgaaa
     8701 tatagtccag aaaagtcttc atagtatttt tcagaaaaat tgttatcttc ctcaaaatca
     8761 tctattcgct tttctaatcg attcaccaga tcctcaatac gatcggcttg ctgtttaaca
     8821 gcaatagatt gaaaatcatt ggtcaaaatt ttactgaata atttactggt tctgattcct
     8881 tctgaattag ccaattgatc gtagaattct gctacttgtg gatctaacct gactgcataa
     8941 gttttgactt tttcgcccat a
//
