LOCUS       NZ_CP074557             2927 bp    DNA     circular CON 22-FEB-2024
DEFINITION  Klebsiella pneumoniae strain S37_CRE38 plasmid pCRE38_4, complete
            sequence.
ACCESSION   NZ_CP074557
VERSION     NZ_CP074557.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387655
            Assembly: GCF_027595745.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Characterization Of Recent Carbapenem-Resistant Enterobacteriaceae
            Isolates From A Large Tertiary Care US Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2021) Biological Sciences, University of Alabama
            in Huntsville, 301 Sparkman Dr, Huntsville, AL 35899, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 330x
            Sequencing Technology             :: Illumina NextSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/22/2024 01:22:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,606
            CDSs (total)                      :: 5,485
            Genes (coding)                    :: 5,373
            CDSs (with protein)               :: 5,373
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 51 of 112
            Pseudo Genes (incomplete)         :: 67 of 112
            Pseudo Genes (internal stop)      :: 14 of 112
            Pseudo Genes (multiple problems)  :: 19 of 112
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="S37_CRE38"
                     /isolation_source="BAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRE38_4"
                     /geo_loc_name="USA: Durham, NC"
                     /lat_lon="36.0368 N 78.9000 W"
                     /collection_date="2017-11-14"
                     /collected_by="Rachel Addison"
     gene            join(2563..2927,1..58)
                     /locus_tag="KHW02_RS27945"
                     /old_locus_tag="KHW02_27845"
     CDS             join(2563..2927,1..58)
                     /locus_tag="KHW02_RS27945"
                     /old_locus_tag="KHW02_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_022644822.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(784..1131)
                     /locus_tag="KHW02_RS27950"
                     /old_locus_tag="KHW02_27850"
     CDS             complement(784..1131)
                     /locus_tag="KHW02_RS27950"
                     /old_locus_tag="KHW02_27850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110958373.1"
                     /translation="MIMILWWISSFITHMMKVGNVREYGPTKSHLLLMVTTLPAGVGVF
                     IYPIVEVIWGDLSLLKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIA
                     GLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(1146..1523)
                     /locus_tag="KHW02_RS27955"
                     /old_locus_tag="KHW02_27855"
     CDS             complement(1146..1523)
                     /locus_tag="KHW02_RS27955"
                     /old_locus_tag="KHW02_27855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110982627.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     gene            complement(1813..1956)
                     /locus_tag="KHW02_RS28050"
     CDS             complement(1813..1956)
                     /locus_tag="KHW02_RS28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058671098.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGNGFAEQRSV
                     EV"
                     /name="hypothetical protein"
     gene            2372..2563
                     /locus_tag="KHW02_RS27960"
                     /old_locus_tag="KHW02_27860"
     CDS             2372..2563
                     /locus_tag="KHW02_RS27960"
                     /old_locus_tag="KHW02_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004207910.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1849..2214)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 gcacgaacct tcttcaccat cccattcccc gtggtggaaa ttctggaaaa aatcctgatc
       61 ctgccatgct tgagttttcc tctccggcca tcccggagag tttctgaacc ttcccaaaaa
      121 tcgccagatc tctgaacgct cctcaccact aaaaataccc acaccccacg caaaattaaa
      181 attttgcaga ttcattattt acaacaatgg gttaatgaaa aataaatgtg cggtgatgtg
      241 ttacaaagtg cagatccaag tcgtggacga ctctatggcg aatcgctgac gaattgccca
      301 cgattcatat gtgaatcacg ctttattatc atattgttat gatgaatcga ctcttgattc
      361 aattcatcac tttgggtgtt ttttgctcat ggtgactctt gattcaattc acgattcgaa
      421 tcgccggtga atcgtgtggt gagtcgtccg gcgacttgcc ataacgtgat tgccggccgt
      481 cgacgaaccc gattcctgaa ggtgctgaaa accggttttc cggctgcgcc ggcaacgctt
      541 cacggccacg caataaattg cgtccccgtt caccgttttc ggaggagaag aacgccggaa
      601 acaggacttc ccctggctga atggtgtgaa tttccgcgct atgcacttgc gcgcatactc
      661 atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc
      721 tgttttaccg gtttcccgtc agaaacgccc tgagggcctc tcaggcggtg ctgataacgg
      781 atgttatggt aaatgttgga tagcaaggac tatcagtccg gcaatgctac atatcattgc
      841 taatggattt acgagcaaaa tgccgaatgt tgtcgcagat aatccgcgaa caatgatatt
      901 ggcaagaagt ccgccaacaa tgagggataa aaaggtataa ccagcaactt tcagtaacga
      961 cagatctccc catatcacct ctactatcgg atagataaaa acaccaacgc cagcaggaag
     1021 cgtagtaacc atcaacagta aatggctttt agtgggaccg tactctctga catttccaac
     1081 tttcatcatg tgtgtgatga atgacgatat ccaccatagg atcatgatca taaggaaatt
     1141 cataattatt tactctcgat gcgtagatca ggtgctttct gtagatttgg tcgtacatac
     1201 tcgctgtctc tttcgtcaag acaacggaag tagagtgtta ctgtggtatc tatccccatt
     1261 cttgtgttag ccacgttttc gttagtgagc aggaattttt taccgaggct attacatttc
     1321 tcagaacctg ttttaaaggc actttgacgt gctgatgccg catctctaaa accatcagca
     1381 gttgctgaaa ccgtatatgt atccctacct aactcaagaa cgttgctggt agaaacacaa
     1441 ccaactgtaa gcagcgatat tgataaaata cctaacctat aaatcattga ttttattacc
     1501 caagttgggt gattgtggat catgattata tctgtttttt ttattattaa aatcaagggg
     1561 ttcaataatt ttcttcaaag aactggtgtc gtggagtggt gctggtttcc ctttgggggg
     1621 gagagggatg gaataggatc ctcttgagat cctttctgtt gcgtcgtaat ctcttgccct
     1681 ggaaacgaaa aaaccgccca gcaaggcggt tttttcgaag gttcttagag cagcaactct
     1741 ttgaaccgag gtaactggct ttcgcagagc acagaaacca aattagttat tacagtgtag
     1801 ctgtacttca cctcacactt caacacttct ttgttctgcg aatccgttac cagtggctgc
     1861 tgccagtggc gataagtcgt gtctttccgg attggactca agacgatagt taccggataa
     1921 ggcgcagcag tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     1981 ctacccggaa ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggagtga
     2041 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg
     2101 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt ccgtttctgc
     2161 gatgcttgtc aggggggcgg agcctatgga aaaacggcaa cgccgtggcc tttccttttt
     2221 ttcctccttt gaccgctcgg ttttctcgag cttttctatg catgctcctg ccgctcgccg
     2281 cagccgaacg accggagcgt agcgactgag tgagcgagga agcggaaaag agacaggttt
     2341 tacactgagc actgacgctc tgaggccctt tatgacgaaa caacagaaaa cggcgctgaa
     2401 catggctaaa ttcatccaga atcagtctct gctcctgctg gagaagctta acgagcttga
     2461 tcttgatgct gaggccgacc tttgtgagaa actccacgat gatgcagagc atcttttccg
     2521 tacactctcc tcgagactgg attcattaca ggatggtaac tgatggccag agtgtctatt
     2581 agtgaagctg cacggcttgt taaggttagt cgtcccacta tttacaagat gattaacagc
     2641 gggaagttaa gttatacctc tgttgtaaag cacgggaagg atattaaagt tattgatgta
     2701 tccgagctta ttagggtgtt tggttctctt gatggtgtaa ctgatactgt aaaatatgat
     2761 gtaaaaagtg acgcagaatc tacaggtatt aacagcgttg gtttacatga tttacaacat
     2821 agaatcgctt tacttgaagc tgaaaacgat ggtcttaaag gggctgttaa ggctcgagat
     2881 gagcatattg attcccttcg tcaggctatg cagcttctgg agcacaa
//
