LOCUS       NZ_CP074547            10121 bp    DNA     circular CON 22-FEB-2024
DEFINITION  Klebsiella aerogenes strain S33_CRE34 plasmid pCRE34_2, complete
            sequence.
ACCESSION   NZ_CP074547
VERSION     NZ_CP074547.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387652
            Assembly: GCF_027595705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Characterization Of Recent Carbapenem-Resistant Enterobacteriaceae
            Isolates From A Large Tertiary Care US Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10121)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2021) Biological Sciences, University of Alabama
            in Huntsville, 301 Sparkman Dr, Huntsville, AL 35899, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 301x
            Sequencing Technology             :: Illumina NextSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/22/2024 01:24:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,328
            CDSs (total)                      :: 5,206
            Genes (coding)                    :: 5,124
            CDSs (with protein)               :: 5,124
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 39 of 82
            Pseudo Genes (incomplete)         :: 43 of 82
            Pseudo Genes (internal stop)      :: 10 of 82
            Pseudo Genes (multiple problems)  :: 9 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074547.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="S33_CRE34"
                     /isolation_source="Lt groin wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:548"
                     /plasmid="pCRE34_2"
                     /geo_loc_name="USA: Durham, NC"
                     /lat_lon="36.0075 N 78.9384 W"
                     /collection_date="2017-12-18"
                     /collected_by="Rachel Addison"
     gene            join(9694..10121,1..304)
                     /gene="mobC"
                     /locus_tag="KHW00_RS26565"
                     /old_locus_tag="KHW00_26500"
     CDS             join(9694..10121,1..304)
                     /gene="mobC"
                     /locus_tag="KHW00_RS26565"
                     /old_locus_tag="KHW00_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004146447.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            311..841
                     /locus_tag="KHW00_RS26570"
                     /old_locus_tag="KHW00_26505"
     CDS             311..841
                     /locus_tag="KHW00_RS26570"
                     /old_locus_tag="KHW00_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515243.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLSDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPSGKK"
                     /name="hypothetical protein"
     gene            complement(869..1048)
                     /locus_tag="KHW00_RS26575"
                     /old_locus_tag="KHW00_26510"
     CDS             complement(869..1048)
                     /locus_tag="KHW00_RS26575"
                     /old_locus_tag="KHW00_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1117..1494
                     /locus_tag="KHW00_RS26580"
                     /old_locus_tag="KHW00_26515"
     CDS             1117..1494
                     /locus_tag="KHW00_RS26580"
                     /old_locus_tag="KHW00_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515245.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETQERDGLRLPGETEASVSEALISEIKALREEVAGLRQEMQAMRLLEH
                     KPETADTHPETSRRWWHWGKR"
                     /name="hypothetical protein"
     gene            complement(1556..1855)
                     /locus_tag="KHW00_RS26585"
                     /old_locus_tag="KHW00_26520"
     CDS             complement(1556..1855)
                     /locus_tag="KHW00_RS26585"
                     /old_locus_tag="KHW00_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_021515246.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHILYDVDDQARKVRVLAVVGQRQNPHTVS"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1845..2108)
                     /locus_tag="KHW00_RS26590"
                     /old_locus_tag="KHW00_26525"
     CDS             complement(1845..2108)
                     /locus_tag="KHW00_RS26590"
                     /old_locus_tag="KHW00_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515247.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRSGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(2454..2717)
                     /locus_tag="KHW00_RS26595"
                     /old_locus_tag="KHW00_26530"
     CDS             complement(2454..2717)
                     /locus_tag="KHW00_RS26595"
                     /old_locus_tag="KHW00_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_010891192.1"
                     /translation="MAAYTSETAKFLQSVSSDQTWWKSRYNPGDSVPHSGIYRCTVCGK
                     EITSNGNDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="protein L"
     gene            complement(3156..3368)
                     /locus_tag="KHW00_RS26600"
     CDS             complement(3156..3368)
                     /locus_tag="KHW00_RS26600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045616503.1"
                     /translation="MDKRTTLVALVSIIFFTSGCDDQKKGNGNAESTQITKNDITTQLK
                     LDKTQQPDDIEKKIKEIQANSTPEK"
                     /name="hypothetical protein"
     gene            complement(3490..3735)
                     /locus_tag="KHW00_RS26605"
                     /old_locus_tag="KHW00_26540"
     CDS             complement(3490..3735)
                     /locus_tag="KHW00_RS26605"
                     /old_locus_tag="KHW00_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515249.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4062..5747
                     /locus_tag="KHW00_RS26610"
                     /old_locus_tag="KHW00_26545"
     CDS             4062..5747
                     /locus_tag="KHW00_RS26610"
                     /old_locus_tag="KHW00_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032427631.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSFSGDALSSAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADTVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPALQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNITTLNGRITSTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNAKLKAAQASLNAMNDALSRDKAALSGAMESRKQKEKKAKEAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5757..6014
                     /locus_tag="KHW00_RS26615"
                     /old_locus_tag="KHW00_26550"
     CDS             5757..6014
                     /locus_tag="KHW00_RS26615"
                     /old_locus_tag="KHW00_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_010891189.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKPWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            6099..6248
                     /locus_tag="KHW00_RS26620"
                     /old_locus_tag="KHW00_26555"
     CDS             6099..6248
                     /locus_tag="KHW00_RS26620"
                     /old_locus_tag="KHW00_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_010891188.1"
                     /translation="MKKAKAIFLFILIVSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            7733..9694
                     /locus_tag="KHW00_RS26625"
                     /old_locus_tag="KHW00_26560"
     CDS             7733..9694
                     /locus_tag="KHW00_RS26625"
                     /old_locus_tag="KHW00_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_021515242.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPNFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QPEARRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..10121
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6352..6932)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..301
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="99.0"
                     /evalue="2.33e-58"
     Typing          9695..10119
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.662"
                     /coverage="99.0"
                     /evalue="2.7800000000000003e-72"
ORIGIN
        1 gatgaaggtg ccggacgcgc tggcggagct ggacggcaga acggtggctt ttgaggtgga
       61 gcgcaccgtg aagagccgca ggcgctatca ggaggtggtg tccgggtatc tgtttaaccg
      121 ccgggcgaac ggcatcgatg aaatctggta catctgcccg gaccgggcga cgcaggtcag
      181 ggtgcagcgg gcgatcctgt cggtggatga aatcgtgaat ccacagaccg gcgaggcccg
      241 caaaacggcg gagctggatc gtgaacggct gtttgcgtgc tttaaattta tgacaacgga
      301 gtgagggatt atggcgctgt acatggtatt tattgaagac gcggaggacc gggacggcga
      361 gcgctgcatc atgccgacca gcgagcatct tgaagatatg gtggtgctcg cctggcagac
      421 cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat ctggcgatga tgaaatccat
      481 cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc acgtactttt tcaccggtga
      541 aaatccgcaa tgggaacggg acaaacccac aggaatgaag ctgagtgatt acctctatcg
      601 caaggtggag cggttcgcca tcgagaacgg tgtgcaggtg gttgatgccg ggaagtgctc
      661 accggagcag attaacgcga tgattgaggt ggtaagcgag cggcacgggg aaagcgcggt
      721 gaacgagaag ctgaaagcgg agctgatgcg ggaatataac agccgctatt ttaacgtgcc
      781 ggtgcctgaa ctgccggagg acaaacccgc ccgccggtgg tggccgtccg gcaaaaaata
      841 aacggcagca ccggcatggc cggtgctgtt attccttccc gagcttatcc cgcatcacct
      901 gccagagtgt ttccgcatcc tcgtgaagcc gctcgcacat gtcggccggg gtatccatat
      961 ccagctcgtt cagtttctcg agcaacagca gcgactggtc cttaatgaat ttagccatat
     1021 tgagggcagt tttttcctgc ttcgtcatgt tttctgtcct tccgggtgtg gtgtgcttcc
     1081 tgtcgggtat actgtctcaa aaccggaggt gagacaatgg cgtgggtgac ggtgagacag
     1141 gcgacggaac tgacggggaa agcccggagt tctctgtatc gtgatatggc gaaaggccgc
     1201 gtatcatacc gtacggaagc tgatggtggt cgggtggtgg ataccagcga gcttatccgg
     1261 gtgtacggtg aattaagaca gggtgagact caggagcggg acgggttgag actgccgggt
     1321 gagactgaag catcggtgtc tgaggcactg atttcggaga ttaaagcgct gcgggaagag
     1381 gtagccggac tgcggcagga aatgcaggcc atgagactgc tggaacacaa accggagaca
     1441 gcagatacac atcccgaaac gtcgcgccgg tggtggcact ggggaaaacg ctgacgcgct
     1501 gcgcttgtcc aaccggcgac ggccacgcaa tgaattgcgc ccccgtcatc ggttattagc
     1561 taacggtatg gggattctgg cgctggccga ccacagccag aacccggact ttccgggcct
     1621 ggtcatcaac atcatagagg atatgctgtc ccatcaggct gatccggcga acgtcctcgc
     1681 catagcgtga gtccacttca tagaggcgcg ggaagtcggc cagtttgtcc gccgcagcga
     1741 gaacgcgatc ggtaacgttc tgcgcatccc acagggatga attttccgca atgtagtcaa
     1801 acaatgctgt cacatcctcg atggctccct gcttccattc cacctcatac ggcatcgcgt
     1861 agttcctgat agcgtccatg cagtctctgg cgaagttgtt ccgtggtcat gccagagcgc
     1921 gtgtcagcgc gggcggcggt cactttagcg cgaacctgat cttcatactc tgcctgggcc
     1981 agttcttcgt tagccaggcg ttgcaacagc gcagctttgt ccgggccacc ttccggcagt
     2041 atgcggggca ggttttcagc cagttcccgc aggaagttgg cgttttcgta gtgtgtagtg
     2101 ttgctcattg cggagcctcc cgggttaatg cggtgatatg aagaagttta gcagggggcg
     2161 gtctgaaaga atatggtcgt gtcagtcagg ctgatgaagc agaaagtcgg taaaaaaagg
     2221 gcttacggca tccattttac gtcaaaacat atgctatgca gttgcgtgca taggcatgca
     2281 ttaggataaa atttaccggg cgcgtttccg gcggttttcc gggtgggttg ttgcttgttt
     2341 tatcccgtag ccgccggaaa cgccgccagt gccttactgg cggtaccgat aagggatgtt
     2401 atggtaaata tcttcgagtt tcaggtacaa aaaaagctcc ggatggctct caatcatttg
     2461 attccgaaac ggtcaccttt agtgtccgtt ctgacaatca gttgccattt gattgcttgt
     2521 tgctgactgt gctgatggtg gttttgcggc gggaacgggt cgttgccgtt actggtgatc
     2581 tcttttccgc agactgtaca tcgataaatg ccagaatggg gaacgctgtc tcctggatta
     2641 tatcgtgact tccaccatgt ttgatcgcta ctgacgcttt gtaaaaactt cgctgtttcg
     2701 ctggtatatg cagccattta gctttccttt tctgaattta taggacagat atagaaaagc
     2761 cgcccgctgg ggcgcccagc ttgcgacgtt atggtaaata ctttaggaac aaatcgatgg
     2821 aagtgccttg tatgtgtaga ttaatttgtc acttaagtag tagttgtaaa tgacaccttc
     2881 aggaaatagc tctctgaata cttttagtct ggcatcgtca gagcagtagc tattttttat
     2941 gtttttttgc atggcatctt tccacacatc actttccatc gtgctggctg aaatcccttt
     3001 ggttacatat ttgtatgaga atgtacctcc gtcagttgat acatctgtca ttaatgtggc
     3061 ttcgtcaacc aatagtggta gttttttctt aatttctgaa acaaccgact ctcgtaggat
     3121 tttagggtcg tcatctttta attcattgaa tttttttatt tttcaggggt ggagttggcc
     3181 tggatttctt ttattttctt ttctatatcg tcaggttgtt gcgttttatc taacttaagt
     3241 tgagttgtta tatcgttttt agtgatttgt gtcgattcag cgttgccatt cccttttttc
     3301 tggtcatcac atccagaagt gaaaaagatg atagaaacca gagctactaa cgttgtcctt
     3361 ttgtccatgt ttttcccttt ttaaatcgta taaacccatt ggattgccga aagcatgatt
     3421 ttcgataata ccatatccct aaaatgcttc cgaagtggat acacctgaaa gtagaacctt
     3481 aaatcgtttt caccggcggc gctgcggctg ccaggtgaag gtggtttccg ccgcctcgta
     3541 tttctcccga cgcatttcct gcgcccgcct ggcttcacgt tcgtcctgaa tggcacgaag
     3601 caccctttcc cgcacctgtt gttttaccgc ctttggcagc gttttgttcc ctttcaggcc
     3661 catggccagt gcctgagcga caaaggcgtc cgccaggcgc ttttcttcag cgggagtcag
     3721 tttcaggtgc gtcatagtgg cctccgggcg tctcaggttt cccgaacagt ttaaccgcag
     3781 gtcctctcct catgccattt cgctttcagg tacatctata ccaatataaa agtaagtgtg
     3841 atgaatatct caaataataa aatttgttgc catgagaaat tttcgggaaa agacgcgatc
     3901 tgcttacagg cggctccggg cgcttttttg tgcctgaaga tgagaactaa tctcatatat
     3961 tgacacctga aaactggagg agtaaggtaa taatcatact gtgtatatat acagtatttt
     4021 ggttttaatt tattgtttta aatgtcaaag aggaaaacga tatgagtggt ggagacggtc
     4081 gaggtccggg taattcaggt ctgggacata atggtggtca ggccagtggg aatgtgaacg
     4141 gtacgtctgg taaaggtggc ccttcatcag gtggtggtac ggatccaaac agcgggccgg
     4201 gctggggtac gacgcatacg ccgaatggag atattcataa ctataatccg ggggagtttg
     4261 gtaacggcgg gagtaaaccc ggtggtaatg gtggtaacag cggcaatcat agcggtagct
     4321 ctggtggtgg acagtcttcg gccaccgcga tggccttcgg tctgcctgcc ctggctacac
     4381 cgggggctga aggactggct ttatcctttt ccggcgatgc gctgtcgtca gccgttgccg
     4441 atgtgctggc tgccctgaaa ggtccgttta agtttggtct gtgggggatt gcgatctatg
     4501 gtgtactgcc ttctgagatt gcaaaagatg atccgaaaat gatgtcaaaa attatgacgt
     4561 cattaccggc cgatacggtg acggagactc cggcaagtac tttaccgctg gaccaagcga
     4621 cggttcgtgt cagacaacgg gttgtggatg tggtgaagga tgagcggcag catattgcgg
     4681 ttgtcgcggg tcggccaatg agtgttcctg tggtggatgc gaaaccgaca aaacgtccgg
     4741 gggtattcag tgtgtctatt ccgggtctcc cggctctgca ggtgagcgta ccgaaaggtg
     4801 ttccggcagc gaaagccccg ccaaaaggca ttgttgctga aaaaggtgat tcacgtccgg
     4861 ctggttttac ggccggtggt aactcccgtg aggccgttat tcgtttcccg aaagagaccg
     4921 gacagaagcc ggtctatgtg tcggtgacag atgttcttac cccggcacag gtaaaacagc
     4981 gtcaggagga agaaaagcgt cgccagcagg catgggacgc cgctcatccg gaagaggggc
     5041 tgaaaagaga atatgataaa gcgaaagctg agctggatgc cgaagataaa aatattacga
     5101 ctttaaacgg caggattaca tcgacagaaa aagcgattcc tggcgccagg gctgctgtcc
     5161 aggaagctga taaaaaggtg aaagaggcgg aggcgaataa ggatgatttt gtgacttata
     5221 accctcctca tgaatatggc tccgggtggc aggatcaggt tcgctatctt gataaggata
     5281 ttcagaatca gaatgcgaaa ttaaaagctg ctcaggcatc tttaaacgca atgaatgatg
     5341 ccttatccag agataaggct gcgctttccg gggcgatgga gagccggaaa caaaaggaga
     5401 aaaaagcgaa ggaggcagaa aataaattaa atgaggaaaa gaaaaagcct cgcaagggaa
     5461 ctaaagatta tggccatgat tattttcctg atcccaagac tgaagatatt aaggggttgg
     5521 gagagttgaa agagggaaaa cctaaaaccc ctaaacaagg aggaggtggt aagcgggccc
     5581 gatggtatgg tgataaaaag cgtaaaattt atgaatggga ttcccagcac ggtgagcttg
     5641 aaggataccg cgccagtgat ggcgaacacc tcggcgcatt cgatccaaaa acgggtaagc
     5701 aggttaaagg gccggatcca aaacgaaaca ttaaaaaata tctttaagag gtaaatatgg
     5761 ggcttaaatt acatattcat tggtttgata agaaaaccga agagtttaaa ggcggtgaat
     5821 actcaaaaga cttcggtgat gatggttctg tcattgaaag tctggggatg cctttaaagg
     5881 ataatattaa taatggttgg tttgatgttg aaaaaccatg ggtttcgata ttacagccac
     5941 actttaaaaa tgtaatcgat attagtaaat ttgattactt tgtatccttt gtttaccggg
     6001 atggtaactg gtaagcaaaa gttatcaagg atgagttgaa atacaggctt ccggtgttca
     6061 cggatgaatg ccgaagcctc tgaatacaag gaaggtatat gaaaaaggca aaggctattt
     6121 ttctttttat attgatagtt tcaggctttc ttctggtcgc atgtcaggca aactatatcc
     6181 gggatgttca gggtggaacg gtggcaccat cgtcctcctc tgaactgacg gggatcgcgg
     6241 ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatcttttg
     6301 ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag tcagttgggg
     6361 aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct gtccttccag
     6421 tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc
     6481 tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttaccgtgct tttccgggtt
     6541 ggactcaagt gaacagttac cggataaggc gcagcagtcg ggctgaacgg ggagttcttg
     6601 cttacagccc agcttggagc gaacgaccta caccgagccg agataccagt gtgtgagcta
     6661 tgagaaagcg ccacacttcc cgtaagggag aaaggcggaa caggtatccg gtaaacggca
     6721 gggtcggaac aggagagcgc aagagggagc gacccgccgg aaacggtggg gatctttaag
     6781 tcctgtcggg tttcgcccgt actgtcagat tcatggttga gcctcacggc tcccacagat
     6841 gcaccggaaa agcgtctgtt tatgtgaact ctggcaggag ggcggagcct atggaaaaac
     6901 gccaccggcg cggccctgct gttttgcctc acatgttagt cccctgctta tccacggaat
     6961 ctgtgggtaa ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc ggagcgtagc
     7021 gaccgagtga gcgaggaggc gcaatttccc cggcacctgc aagacggtca ccggacgcgc
     7081 tgatgtggga tattgtcccc tgattacgtc tttctattcc ctgacgatca attatcagga
     7141 taggtgaacc atgtcggttt ttcgctgaac attttacgtt aacctatctc tgttgctttt
     7201 ttctccttgc ggagaaatgc aagtcatccc cgctggggcg tggctgactt cagggggaaa
     7261 tcccctgctt acgctggctg gacgccaccg acgcacggtc tttccccctc tttctggctc
     7321 tgtccggctg gacgccggcc acgccagaaa ttcacccggc ccccgtcagg ctcaccccga
     7381 ccacccacaa aaatccacgg tgctggacgc acctgacgac gcagcaaagc tgcctctgat
     7441 tttgcgggtg gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa
     7501 gaccgcactg gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg
     7561 gggctgcgcc ttttccgcat ggggcggaaa gaggctggcc tccgtggtga aagctgaacg
     7621 cagtcaccac ggacgtgaac gccgccgcca tcccttgggg catgtccgcg gcttgtgcag
     7681 cgggggctgc acaccacgcc ggtaaacccg tcgtctgaag gagagagatt acatgtttaa
     7741 tacggattcg cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg
     7801 ggtcagctgg ctttacaggg agtattacgg tctggccggg gctttcctgg ttatcggcct
     7861 gtttttcggt ctggctgaac tctgggccgg acggatgcgt tttgtggggc tgagtcataa
     7921 ggagcgacgg gagtatgccg atcgctataa gcgcgataat aaccggactg cgccattttc
     7981 ggtgctggcc tcgctggatt cgcccgggcg gatggtcagg gcacggcggc atgtctggaa
     8041 ctacgcgtac tggctgaccg gcgctgccgg tctggccctt ctgggggggc tggttgtcgc
     8101 cgtgtttatc gagagctggg gagaaaccgt cctgcgtccg gacctctacg cgaacgcaga
     8161 ttattacctg ctgcagccgg ctaacctgtt cgggattgcc tggtgcgcgg gcgggctggt
     8221 tgtcgggatt gtgttggcgc ggctgctggc gttcctgctg cttgacggtc attttgcggc
     8281 tgctgacgag gcggtgaacg cgaaactgaa ccaggagagc cggcgcagca gccagcggac
     8341 cggtgaaatg accgacgtcc gccacctgca tttcggggag ccggtaccgg tcaacgcgct
     8401 ggccgatttt tccacggagc aggcccggaa acagcaggcg gtgtttctgg gtaaggatga
     8461 gcagggccag ccggtgctgg tcccgcgtga tacctggcgc aagaccaaca ttcagatcct
     8521 cggcctgccc gggagcggca agagcgtgat ggccaccaac gcgctcattc gctgcgtacg
     8581 cgatttcggg gacgcggtgg tgtacttcga ccccaaaggg gacgcgtggg cgccgcacgt
     8641 cttccgggcg cactgcccaa acttcaccct gctggatctg cggccgggga aaccggcgca
     8701 gctgaatctg ttccgcgatc tcgaccagta cgccctgaag aatctgctgg ttgccgggtt
     8761 taacctgagt gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc
     8821 gaagctgatt gcggagcagt ttccgcaggg ggcaaacatt cagcaggtgc tggcggcggc
     8881 gtacgcgctg ccggaggccc tgaaaaagga cgtgaagggg ctgatcacca agctggagaa
     8941 cgtggccgac ctgagcgtcc tgcagacgga cagcggcatc gacgtggcgg ggataatcaa
     9001 cggcggcggc tgcctgtacg tcatcggctc gatggacgat gaggcggtga tccgggtgca
     9061 gaagatgctg tttgcccgct gcgcacaaat catcattgcg cgggatgagt tccggaggtg
     9121 gccgcacgcg agcatcatgc tcgatgagat taaatacctg ctgtcgaagt acgtgctgaa
     9181 cgcgctgggg acgctgcgca gccgggactg caacctgctg ctcgcgcacc agtcgctggg
     9241 cgacttcggg cagtgcggcc aggatctgcc cgctgatttt gtgaaaacca cggtgctgga
     9301 taacacgccg atccgctggt tctaccgggc ggccagccag gagtccgcgc agtgggcggc
     9361 gggccagacc ggggaaatcc gggtggacgt ggagcgccgc cgggccagcc gggaggcggg
     9421 gaacgtggag catatcagcg gggacagctt catccagaag gaggcgcggc cgctgtttga
     9481 cgtgaacacc ctgcaacatc tgcctgacgg gttcgcggtg atgaccgggc tgggcgtggc
     9541 gcggctggcg ttcagcagcc cgctgcgggt tgatcgccgg gaaattccgc tgaaaacctt
     9601 cccggtgctg gcgaaaacgg acccgctggc ggagtaccag ccggaggcca ggcggcagcg
     9661 gccgggtgat gatgattttg cggggctgta ctgatggcac tggagcgata caacgtcagc
     9721 cacgcgaagc ggcaggcgcg gaacgcggag aaaacacggc tgaccctgcg ctggctgcgg
     9781 gaagagctgt gttccacggc ggagctggtt gcgcgacggc tggggattgc ggcggtacag
     9841 ccggtttacc gcttcctgga cagcctggtg gcgaaagggc tgctggtccg ggcgaaatac
     9901 ccggttgacg gccgtcaggt cagcgtgtgg gggctgacgc cgcacggggt ggcgttctcg
     9961 ttcgacgagg acgagccgct gacggacatt attccgtttc agccgtcgcg ggtgtcggcg
    10021 gcgcagctgc cgcaccggct ggccgtgcag tccctgcgcc tcgccatgga ggcccggggg
    10081 gccaccggct ggcggtacct gcaccgtatt gccctgaaag g
//
