LOCUS       NZ_CP074534             2175 bp    DNA     circular CON 22-FEB-2024
DEFINITION  Klebsiella pneumoniae strain S14_CRE14 plasmid pCRE14_6, complete
            sequence.
ACCESSION   NZ_CP074534
VERSION     NZ_CP074534.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387634
            Assembly: GCF_027595625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Characterization Of Recent Carbapenem-Resistant Enterobacteriaceae
            Isolates From A Large Tertiary Care US Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2021) Biological Sciences, University of Alabama
            in Huntsville, 301 Sparkman Dr, Huntsville, AL 35899, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 360x
            Sequencing Technology             :: Illumina NextSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/22/2024 01:43:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,724
            CDSs (total)                      :: 5,603
            Genes (coding)                    :: 5,439
            CDSs (with protein)               :: 5,439
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 60 of 164
            Pseudo Genes (incomplete)         :: 104 of 164
            Pseudo Genes (internal stop)      :: 29 of 164
            Pseudo Genes (multiple problems)  :: 25 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074534.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="S14_CRE14"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRE14_6"
                     /geo_loc_name="USA: Durham, NC"
                     /lat_lon="36.0075 N 78.9384 W"
                     /collection_date="2017-07-13"
                     /collected_by="Rachel Addison"
     gene            152..388
                     /locus_tag="KHV96_RS28575"
                     /old_locus_tag="KHV96_28450"
     CDS             152..388
                     /locus_tag="KHV96_RS28575"
                     /old_locus_tag="KHV96_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042863677.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFVWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(654..1079)
                     /locus_tag="KHV96_RS28580"
                     /old_locus_tag="KHV96_28455"
     CDS             complement(654..1079)
                     /locus_tag="KHV96_RS28580"
                     /old_locus_tag="KHV96_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_015328245.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMNSKQQ"
                     /name="PH domain-containing protein"
     Typing          1991..2163
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          complement(1528..2044)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.692"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgacgcgcct gcggcttgtc caacgcctca cggcaccgga ttaaaatccg ctcccgttca
       61 ccgtttctga ctgcctttca ccgtcgctta cgcttcatcg ttcctactgt gtctggcaga
      121 ctgctgggac tattctcata cggagataaa catgacgaga cttacaaacc tgacccccgc
      181 tgagaaaaag tttatcgacg acgctatagc cgctgccgaa cgtgcggccg ggaagaagct
      241 caatcagcct aaccggcaca tcgttctgaa cagagcgcgt gcgcagattg agtcgcagcg
      301 ttacgccgac cgccagcgtg cattacgtga ggatgagcgg cagcagtccg attttgtctg
      361 gtcacgtccg cgcgcgccgc gccgctgatt gcggattttg gcggtttagt ggtgctgacg
      421 ctgcgaaatc acctgcgatt cacccgcgtg cataacgatt catgcgctca aaaatcacca
      481 gagcgtttct gaacgttttt caggaggtca tacgtgttct tttgcgtatt agggcggaga
      541 aacgccctga gagcctctga ggcggtgcgg gcaacgggtg ttatggtaaa tccagctgat
      601 tggcaggtac aaaaaaagcc ccgtagggct ttttttgtaa tgctacgagg aagctactgc
      661 tgcttagaat tcatttcctc gatatgattg ttcacaatcg atctgaattg cattggcgcc
      721 ttgatgtttg gaattggagt gttggttccg ccagtgcctt taatcacgat agacccatag
      781 ttaaagattc gtcccagtat cccttgatgt atgccaaggc tttcaacttt atcaatacgg
      841 agttccaccg tattgcgtcg gatcaaaccc acttttgcga tgactctttg gttagttaat
      901 gcaagctccg ttgaccaaac tctaaggagc acagggatta ttataagtaa ccctaagccg
      961 taaaaaggca gcaacaagat tccgataatt actgggatga ttgttggaat ccaggaggtt
     1021 tgagcgcgag atattacgga ttcatttctt gtcaatgatt ggtctacgta gcttcccata
     1081 aggttatatc catttagaaa aaataggatt tttccctttt taaggcgaat cagcaataga
     1141 aactttatgt ctccgtggta ggtatacaaa atttacggag agcgtagcga ccgtcgcaca
     1201 gttcggttct ttcctttcgt gccgcaactg gcttgattct gtgatggtgg tggtggctgc
     1261 tgctatgtct catatactct tggcgttgca atcctttatc ccatatctgt cgaatatcac
     1321 agatgatctt cttgagatct tgtccgttgc gtcgtaatct cttgctctgg aaacgaaaaa
     1381 accgccctgc aaggcggttt ttcgaaggtt ctcagagcta ccaactcttt gaaccgcggt
     1441 aactggcttg gaggagcgca gtaaccaaat ctgtcctttc agtttagcct taacaggcgc
     1501 ataacttcaa gactaactcc tctaaatccg ttaccaatgg ctaccgccac tggcgatttg
     1561 tcgtgtcttt ccgggttgga ctcaagttga tagttaccgg aacgggcgtg gtagtcggac
     1621 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt
     1681 gtcaggcgtg gaatgagata aacgcggcca taacagcgga atgacaccgg tacaccgaaa
     1741 ggcaggaaca ggagagcgca cgagggagct accaggggga aacgcctggg atctttatag
     1801 tcctgtcggg tttcgccacc actgatttga gcgtcagatt ctgtgatgct tgtcaggggg
     1861 gcggagccta tggaaaaacg gcagcgccgc atcctttttc tgccccgttt gtcatggcat
     1921 atgcctttgc tcttctccgt gtgcaggtaa cgcccgccgc agccgaacgg caggagcgtc
     1981 cgcgactgag tgagcgagga ggcgtcatat cctctgaact tccattttct gccttacgcc
     2041 cgcagctgca ttttcctctg tcacatgaag cactttcctg attaccgcac tcatgccaac
     2101 ataataagcc agtatacact ccgctagcgc tacgtgactg gttcagggct gcgccccgaa
     2161 acccgctaaa tccac
//
