LOCUS       NZ_CP074533             3511 bp    DNA     circular CON 22-FEB-2024
DEFINITION  Klebsiella pneumoniae strain S14_CRE14 plasmid pCRE14_5, complete
            sequence.
ACCESSION   NZ_CP074533
VERSION     NZ_CP074533.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387634
            Assembly: GCF_027595625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Characterization Of Recent Carbapenem-Resistant Enterobacteriaceae
            Isolates From A Large Tertiary Care US Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2021) Biological Sciences, University of Alabama
            in Huntsville, 301 Sparkman Dr, Huntsville, AL 35899, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 360x
            Sequencing Technology             :: Illumina NextSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/22/2024 01:43:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,724
            CDSs (total)                      :: 5,603
            Genes (coding)                    :: 5,439
            CDSs (with protein)               :: 5,439
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 60 of 164
            Pseudo Genes (incomplete)         :: 104 of 164
            Pseudo Genes (internal stop)      :: 29 of 164
            Pseudo Genes (multiple problems)  :: 25 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074533.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="S14_CRE14"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRE14_5"
                     /geo_loc_name="USA: Durham, NC"
                     /lat_lon="36.0075 N 78.9384 W"
                     /collection_date="2017-07-13"
                     /collected_by="Rachel Addison"
     gene            complement(712..894)
                     /locus_tag="KHV96_RS28550"
                     /old_locus_tag="KHV96_28425"
     CDS             complement(712..894)
                     /locus_tag="KHV96_RS28550"
                     /old_locus_tag="KHV96_28425"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            complement(1289..1504)
                     /locus_tag="KHV96_RS28555"
                     /old_locus_tag="KHV96_28430"
     CDS             complement(1289..1504)
                     /locus_tag="KHV96_RS28555"
                     /old_locus_tag="KHV96_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1511..2008)
                     /locus_tag="KHV96_RS28560"
                     /old_locus_tag="KHV96_28435"
     CDS             complement(1511..2008)
                     /locus_tag="KHV96_RS28560"
                     /old_locus_tag="KHV96_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2049..2747)
                     /locus_tag="KHV96_RS28670"
                     /old_locus_tag="KHV96_28440"
                     /pseudo=""
     CDS             complement(<2049..2747)
                     /locus_tag="KHV96_RS28670"
                     /old_locus_tag="KHV96_28440"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2686..3027)
                     /locus_tag="KHV96_RS28570"
                     /old_locus_tag="KHV96_28445"
     CDS             complement(2686..3027)
                     /locus_tag="KHV96_RS28570"
                     /old_locus_tag="KHV96_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3113..3286)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          2..469
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.451"
                     /coverage="100.0"
                     /evalue="3.78e-134"
     Typing          3210..3511
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.508"
                     /coverage="92.0"
                     /evalue="5.900000000000001e-103"
     Typing          complement(1174..2696)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2693..3006)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="9.860000000000001e-59"
ORIGIN
        1 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
       61 ctgttatggc cgcgtttatc tcattccacg cctgacactc agttccgggt aggcagttcg
      121 ctccaagctg gactgtatgc acaaaccccc cgttcagtcc gactgctgcg ccttacccgg
      181 taactatcat cttgagtcca acccgagaaa gcacgacaaa gcgccactgg cagcagccac
      241 tggtaactgg gatatacgag agagagattc agaggtcttg aagtatgggc ctgactgcgg
      301 ctacactgaa agaacagttt tggtatctgc ggtctcgtat acccagttac ctggttaagc
      361 agttccccaa ctgacttaac cttcgatcaa accacctccc caggtggttt tttcgttttc
      421 agagcaagag attacgactg aaacgacagg atcacaagaa gatcattaac tccgagagta
      481 gtaactccca ccatgtttgc ctatcaacac acttaggaaa cattacttgc gtttagttat
      541 acgacttcta gctctccata acagatcctc attttctgct gatggatttc gacggtatcg
      601 ttcctccgct ttccgtactc catattcagg aaacaccctg acagcaacca tttgtataaa
      661 gacataacac accaaaagta aaaataaaaa accaataaac tcaaacatat atcaccctct
      721 tttcttctca tttctcatgc gtgtagccac cattatttta tagttagcac tcgtagggtt
      781 ctccatatat ttacgggtag cacgttcgac ggcagattcg gatgaactaa aaaaaagcat
      841 aaataattta aatgcaataa cggcgactac cactacacca ataaatgtaa gcatcactct
      901 tactcctgtt atcaacatta aacataacat ctcttacccg caccgcgccg gaggcactca
      961 gggcgtttct gatggctgac gggcaaaagg tggtggcaag tacaccgaaa accaccagaa
     1021 acgcacctga tttattttac gcatatgcat cacttattca catcactgca tagccggtga
     1081 tttcggttaa aaaagactct ctgtgtgctg aaatcgcaga aaatggccat gatccccgtc
     1141 acgctcaggc cacctgagac gggcgtttat cagtctccat actccctgtc accatgagat
     1201 aatgtgcgtt gccgttccgg taccagatct gctgtcttct ctggttcctt ttccgttgtt
     1261 tgcccgtatc gctgttgctc aatctcattc agcctgtcga taaccggcgc agctgctcgc
     1321 tcaaactctg cacctgcctg ctcaagcccg acacgcgctc gctcagcgcc gcgttctccc
     1381 gttgcataag accagacatc ttccgccatt cctcgaaggc gctctcccac tcgtccagcc
     1441 ttctcgagta gtcctgctgt agctgctcta acgcgctcag caattgtttt tccagttccg
     1501 tcattctgct ctactcccgt accagcttca ccgcgttccg cgtcttgttg tccaccgtcc
     1561 ggcccgtttc ggctttcatc ccctccggca gcaccagaaa ccgtccgttg cggttctcca
     1621 gataggtcac gccccacgtc tgcatgtgca gtttcgacag cgtatctctc tgctgcctga
     1681 tttccgccag gttcgccgct atccggttcc cctggtacca cagcaccccg ctcaggctgg
     1741 cgaacagcag ccctgacacc atggtgatgg tcagccacgt ccgcccgacc attcgcagca
     1801 cgctcagccg gttcgactgc atcgccgcgt tcatcccctg ttcgtgggcg ctgatggcgt
     1861 cgttgattct cttcgcgctt gcgttcagtt ccgatttgac aacgttctcg tgcttgctga
     1921 atgcggcttt cagcctctct tcggtaagct gctgctgtgc tttcgatttc tcctcgaagt
     1981 ccctcgaggc cgttaaaaga ctgctcataa atggctcccc tcagtcggat atttcgcccc
     2041 ccgtccgggt cggcaatgct gatactgttt tttgtggttc tgaccacctc aaaaccggcc
     2101 gctgtgagcg cccctgtgac gtcatcacgc gttttcagct ctcctgatga ggctaacatc
     2161 agcaaacccc gtgtaatcgt ctctgcggct tcctgcttcg tttctggcag tcctgaaggc
     2221 gtgaccagcg cccgccggtt ctccgggtcg ttcggatcat gtagccccag cctgccgttg
     2281 accactgtct gccacgcatc gatgcgcggc cggtcggcac gatcgtaata cggctggagg
     2341 cgtttccccg tcagtaattc ggtattgggg atcagaaaat tcagctcaag acgacccttg
     2401 tctgcgtgtt ccacccacag aatgctgtac tggtctttat cgagtccggg catcagaacc
     2461 cgctcaaagc tggccatcag tttttcacgc tgacccggcg gcaggtccgc ctccgcgaat
     2521 gacagcaccc cggaggtata ttttttcaca tacggcgacg catcaatcag ctcccggact
     2581 tcttccggct tcccctgcag gacgctggca ccttcacgct gccggtcttt acccagaaga
     2641 taatccacag gaccggcgcc cccgccgcga ccccggggat ggaatttaac aatcatcgtc
     2701 cggccctttt tccagtacgg catgccgcaa ccgttccaga ccggcatcga tcgccatcag
     2761 cgctgccacg atctgcaccc ggtcatgacc ggcaccaccg gcgttcactt tccgggcgat
     2821 ctggttcagg ttattgccca tgccggcaag ctgacgcagc agcgcgggcg aaatcgaggg
     2881 aagcctgccg gcgcgtgtcg gtttctcatc aaggcaggtc tggcgcatcc acgccgccag
     2941 ttgttttccg tcgcaccgct caagcaaccg ccggtgttcg tcttccgtca cccacatcgt
     3001 gagcatcttg ttgcgtttat ctgccagaat cgcctcctga taacgccgtg acggacggga
     3061 aagcctttcc cggtcggcgc ggggttggac aagccgcagg cgcgtcagtg gggtttcggg
     3121 gcgcagccct gaaccagtca cgtagcgcta gcggagtgta tactggctta ctatgttggc
     3181 acggatgagg atgtcagtga agtgcttcat gtggcaggag aaaaaaggcg gcaccggcgc
     3241 gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct
     3301 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
     3361 atgccaggaa gatacttaac agggaagcga tagggccgcg gcgaagccgt ttttccatag
     3421 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
     3481 gacaggacta taaagatacc aggcgtttcc c
//
