LOCUS       NZ_CP074532            11145 bp    DNA     circular CON 22-FEB-2024
DEFINITION  Klebsiella pneumoniae strain S14_CRE14 plasmid pCRE14_4, complete
            sequence.
ACCESSION   NZ_CP074532
VERSION     NZ_CP074532.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387634
            Assembly: GCF_027595625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11145)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Characterization Of Recent Carbapenem-Resistant Enterobacteriaceae
            Isolates From A Large Tertiary Care US Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11145)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2021) Biological Sciences, University of Alabama
            in Huntsville, 301 Sparkman Dr, Huntsville, AL 35899, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 360x
            Sequencing Technology             :: Illumina NextSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/22/2024 01:43:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,724
            CDSs (total)                      :: 5,603
            Genes (coding)                    :: 5,439
            CDSs (with protein)               :: 5,439
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 60 of 164
            Pseudo Genes (incomplete)         :: 104 of 164
            Pseudo Genes (internal stop)      :: 29 of 164
            Pseudo Genes (multiple problems)  :: 25 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074532.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11145
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="S14_CRE14"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCRE14_4"
                     /geo_loc_name="USA: Durham, NC"
                     /lat_lon="36.0075 N 78.9384 W"
                     /collection_date="2017-07-13"
                     /collected_by="Rachel Addison"
     gene            join(10700..11145,1..239)
                     /locus_tag="KHV96_RS28490"
                     /old_locus_tag="KHV96_28360"
                     /pseudo=""
     CDS             join(10700..11145,1..239)
                     /locus_tag="KHV96_RS28490"
                     /old_locus_tag="KHV96_28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            703..813
                     /locus_tag="KHV96_RS28495"
                     /old_locus_tag="KHV96_28365"
     CDS             703..813
                     /locus_tag="KHV96_RS28495"
                     /old_locus_tag="KHV96_28365"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_138663973.1"
                     /translation="MDWILLAIAIALLVVAVYSGISYFREKKSISRVFRR"
                     /name="small membrane protein"
     gene            complement(1186..1392)
                     /locus_tag="KHV96_RS28500"
                     /old_locus_tag="KHV96_28370"
     CDS             complement(1186..1392)
                     /locus_tag="KHV96_RS28500"
                     /old_locus_tag="KHV96_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047359279.1"
                     /translation="MKLITVDESVVNAIVEYYAQGSEEAHAEGNRMFLRYLHELTGTQR
                     VAVEAARKQSLAAINKGGALPCD"
                     /name="hypothetical protein"
     gene            1972..2193
                     /locus_tag="KHV96_RS28505"
                     /old_locus_tag="KHV96_28375"
     CDS             1972..2193
                     /locus_tag="KHV96_RS28505"
                     /old_locus_tag="KHV96_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947202.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            2186..3073
                     /locus_tag="KHV96_RS28510"
                     /old_locus_tag="KHV96_28380"
     CDS             2186..3073
                     /locus_tag="KHV96_RS28510"
                     /old_locus_tag="KHV96_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064179688.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKD
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAPDT
                     RPAADSKFAGREGVIYTVQGKGGLVREVLIPSPLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="hypothetical protein"
     gene            3070..3768
                     /locus_tag="KHV96_RS28515"
                     /old_locus_tag="KHV96_28385"
     CDS             3070..3768
                     /locus_tag="KHV96_RS28515"
                     /old_locus_tag="KHV96_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064179687.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYSGAEDVLREAFAKKENRWRQALKNAREEARQELEEQTEALNQRAS
                     GLLVQEQTLKARMEEVRKYVAGERHNAMRARLEAEEAEKRRQNASATAERRRRKLEKLQ
                     ATDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(3775..4179)
                     /locus_tag="KHV96_RS28520"
                     /old_locus_tag="KHV96_28390"
     CDS             complement(3775..4179)
                     /locus_tag="KHV96_RS28520"
                     /old_locus_tag="KHV96_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064179686.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFVPVSVEQEGEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEKRQNTTRPRAWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4179..4370)
                     /locus_tag="KHV96_RS28525"
                     /old_locus_tag="KHV96_28395"
     CDS             complement(4179..4370)
                     /locus_tag="KHV96_RS28525"
                     /old_locus_tag="KHV96_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4781..4894
                     /locus_tag="KHV96_RS28665"
                     /old_locus_tag="KHV96_28400"
     CDS             4781..4894
                     /locus_tag="KHV96_RS28665"
                     /old_locus_tag="KHV96_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            5346..>5968
                     /locus_tag="KHV96_RS28530"
                     /old_locus_tag="KHV96_28405"
                     /pseudo=""
     CDS             5346..>5968
                     /locus_tag="KHV96_RS28530"
                     /old_locus_tag="KHV96_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011073174.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /name="IS5 family transposase"
     gene            <6134..7101
                     /locus_tag="KHV96_RS28535"
                     /old_locus_tag="KHV96_28410"
                     /pseudo=""
     CDS             <6134..7101
                     /locus_tag="KHV96_RS28535"
                     /old_locus_tag="KHV96_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341074.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(7510..8388)
                     /locus_tag="KHV96_RS28540"
                     /old_locus_tag="KHV96_28415"
     CDS             complement(7510..8388)
                     /locus_tag="KHV96_RS28540"
                     /old_locus_tag="KHV96_28415"
                     /inference="COORDINATES: protein motif:HMM:NF025663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="WP_158236426.1"
                     /translation="MIKFFFGKAYRLLLENLSTTNRVKLRYFRVYKKLPNIKKPKTFNE
                     KVISRILGEQKDIYSTLADKYLVRRFVSERVGEEYLIPLHLATSNPSDLLKIDDWSGLV
                     IKPNHAAGMIYISDDNPSKEEKDNVVTTAKTWLKTDFSSFCDEMHYSKIKPMLLVERKI
                     TKRGEIPRDYKFHCFKQKNGDINFVLQLVDGRFGQESRSYFLNSFENMVWSHGAGNHLL
                     SNNEVAVLNEVILLNKRLILNDFNYVRIDWYILGDRIYFGEITFTPGAGIGNEFGPALE
                     KQMAEWWEDEY"
                     /name="ATP-grasp fold amidoligase family protein"
     gene            complement(8669..9892)
                     /locus_tag="KHV96_RS28545"
                     /old_locus_tag="KHV96_28420"
     CDS             complement(8669..9892)
                     /locus_tag="KHV96_RS28545"
                     /old_locus_tag="KHV96_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512451.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_227513120.1"
                     /translation="MMAEKLVSIIGLVFINSYMAKYIGPTDFGKITFATTIFTFVQTMA
                     WFGGQNILFKRMSEKRKSGIALALHTQKQRRVIFALSSLIALFYLFFFTDTLTFVFGVA
                     NCIATYYIVMDVFAVYNNTQLISRINALTNGAGLCIAFIVRFLISYLELPVYTFSIPII
                     LIPAIPYVIRYIYFHNTIEDTAVLKSKTKKYNKYLFSAGGSLLLSTLSIVIYTQVSNIF
                     LAKITSYSDLGIYNVALTLGSAYSFIPVALITSFFSKIYQEKDFEKMSRLLTLINRIVI
                     LITIFILFGFYFLGPYIISMLYGSAYKLAVELVPIIILATMFSSLGTICYRFIIKYSGY
                     KYLSYKMTLTSLLSIPFSYVMINRYDVFGAAYCFLVVEVLSCTLLNYFFRNGMILRIHL
                     NVIGLMRR"
                     /name="oligosaccharide flippase family protein"
     Typing          4419..4894
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.286"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 tgctgtcggc ctttgaggtc gtggtatgga tgacggatgg ctggccgctg tatgaatcac
       61 gcctgaaggg aaagctgcac gttaacagca agcgttacac tcagcgcatt gagcgacata
      121 acctgaatct gagacaacat ctggcaaggc tgggacggaa gtcactgtcg ttctcaaaat
      181 cggtggagca gcatgacaag gtcatcgggc attatctgaa cataaaacac tatcagtaag
      241 ttggagtcat tacccacgtc tcgatatcac cacccacagc ctttacgcct ggataaagaa
      301 gtacggtccg gataccggat cccgcgggca cgtaaaggcg aggccagtat cgtgtcgccc
      361 aacaggctcc agcgacagtt caatccggat gctccggatg agcgttgggt aacgccccaa
      421 taggcagtgt aagttaactg ccgctgccgc gttttacggc agtggttgca gacaagcagg
      481 ggggggaaca tgcccataac tcatgcacgg tttttttgtt tttcctgtat gtacaaaaaa
      541 cataactagg aataatctta gcagccttaa caaacacaca cacaaacagc ggttatgctt
      601 catctcatta caataagtaa cgacagtatc atgctaaaaa aaagatgata ttatctttat
      661 gaaatcataa caggctgttt ttgttaaatt aatcagtaat ttatggactg gattttactt
      721 gctatcgcca ttgctctact tgttgtagct gtctactcag gaatctcata cttcagagaa
      781 aaaaaatcaa tcagccgtgt atttaggcga tagcgcccta tttaccataa tcccttctta
      841 cgcgcaccgc gaaaacggcc tcagggcgtt tctggcagga aaccggtaaa acaggcaaca
      901 aaccacccga aaacccgcca gaaacgcgcc tgatttattt tacgcatatg catggctatg
      961 cacaccaccg catagccggt gatttcggcc gagaaaaggg ccccggacgt taaaagcata
     1021 aaagaacggg ccatccaggc ccgtcaaatg aggacaactc tgtaaaagga ccgtcgcacc
     1081 tggtgcggtg ccggcgacaa gaaaaaggta gctcatatca gcccctcacc ggagaaaaat
     1141 atttccgggc cagtgcacag acatcagccg tccataaatg cgattttaat cacagggaag
     1201 cgcccccccc ttatttatcg cggccagaga ctgctttctg gccgcctcaa ccgccacacg
     1261 ctgtgttccc gttaactcgt gcagataacg caggaacatc ctgtttccct ctgcatgcgc
     1321 ctcctcactg ccctgtgcgt agtattccac tatcgcgttc accacggatt catccaccgt
     1381 aatcagcttc attctgtctc tccgctttct ctgtgacacc atcctggcat cacgacggtc
     1441 atcctggcgt gaaaacggtg accgggcagg caaatttttt gtctgtccgg tcaccgttgg
     1501 cggggtccgg ggcggtcagt cccggcggtt tttattccgc caaatcagca cctgcgacac
     1561 ccgctatgta cgacgcataa aatgcgcctg tacatagcgg gtgtcgctat tgagcggagt
     1621 caggcgcaac atttaaaaaa caacattcac acgcacgaaa aacctggcta gtatttagtc
     1681 aaaccatgag gaggcgcact ctgacgcgca cctgcctacc ggcagcaggt aaaacagccg
     1741 ggtccctccg gggatactgt accgggcagc ccaatccgag aatcctgaga aaacagctaa
     1801 aatctgcgag accggcaatc aggtcaggcc attccctggc ttaagggacg ttatcagccg
     1861 cgagcagtcc gggctgaacc acgagaggtt cgaacgaggg cgatcggcgc atgccgcagt
     1921 gaccgttaac gtaacggcca ctgatgttct tacacccaat ccggagcaca gatgcaattt
     1981 gataccgctg ccgaactggc cgccctgcag gcgcagaccc gccgtatccg ccaggtccgc
     2041 taccgtccct cccgccttga ccgctatacc ggcgaactgc tgagcctgta ccaggccggg
     2101 gccagtgcag cggagctgca acgctggctc cgggcccgga gaatcaaagt ggtgttgtcc
     2161 acggttaccc gctggctgga aaaaaatggc taaattcgcc agcgctgccc gccaggcggc
     2221 tggcgtgata aaaccactcc agggaaatat tctgcgctct gtcggcacag tccggaatta
     2281 cgagcagggc ctgacccgcg tcgcagaatg gatgaaagac aaccggatta atcacggcga
     2341 tttacgcaca ctgacaccgg cgcaggccat cacctacctg gagcagcgcg gggagcaggt
     2401 cggccagaaa acgctcgata tggaacgcca ggccatccag gccatgatga tccacgtgac
     2461 gcagaccctc cctgccggcg aacacctgcc ggtggtgaaa tcggagcagg cacagatcct
     2521 gagcagccgg gcctacagcc cggaccaggt agccctgatt gccgcagcac aacagcccgc
     2581 caacgctctg gccaccgcga ttgctctggc cgccggatta cgcgcccatg aactgctgac
     2641 cctcgcgccc cgccaggagc gtgcaccgga tacccgtccg gcagcggaca gtaaatttgc
     2701 cggtcgggag ggtgtgattt acaccgttca gggcaaaggc ggcctggtca gggaagtcct
     2761 gataccgtcc cccctggctg aacgtctcga gcatgtcagg ctggcgagcc cggtccgggt
     2821 gaccgatcgc ggcgtgtttt accagagtca gtacgccatt ggcggaggac agcgctggtc
     2881 gaacgccttc agtgccgcct ccatgcgtac cctgggctgg tcacgggggg cacacggcgt
     2941 gcgtcacagc tacgcccagc aacggatgca ggagctgcaa aaactcggtc tggttcgtga
     3001 tatggccctg cgtaccgtga gccaggaaat gggccatttt cgtccggaaa tcacggagac
     3061 atacctgcga tgacaagaac gtttatttta ttgtgtaccg gtgttttcgc catcgcgtac
     3121 gccctgtatg agctgaaaca tatgggcgtt tactatccgg atgccagcaa tccgcagctg
     3181 ttcactggct ggaaggcggt caccgaactc ggtcccgcaa tcctggctac ggggatcgca
     3241 gccacagcgt tctcgctgtg gctgttcatt ttgtttttca gaggctcaga gtcccgtcgg
     3301 atacagcgcg agataagcgg atatcagcag gcactaaggg aggcagaaga ggctcacaga
     3361 gccagacaaa atacccttat tcagaatcac aacctggaga cagcggccct gcgaagccac
     3421 tacagtggtg ctgaggatgt actgcgggaa gcgtttgcga aaaaagaaaa ccgctggcgt
     3481 caggcattga aaaatgcccg ggaagaagcc aggcaggagc tggaagaaca gacggaggca
     3541 ctgaatcagc gcgcgtccgg cctgctggta caggagcaga cactgaaagc cagaatggag
     3601 gaagtcagaa agtatgttgc cggagagcgg cataacgcca tgcgggcccg tctggaggca
     3661 gaggaagcag agaaaaggcg acaaaatgcc agtgcaacag cggaacggcg cagacggaaa
     3721 ctggaaaaac tccaggccac tgacggaaca gcagggcaaa aacactgagg catttcagga
     3781 ctgccagaaa cgccaccagg cacggggccg ggtcgtgttc tgccgcttct cttcccggtt
     3841 ctccagcaac aacatcgcct gtttcagact gtcgatatgt gaatctctgg aggcaaggag
     3901 ggtgttcagg tgttcaattt cctgtttcag aatgtaaatg acctgttcat tacggtcaga
     3961 agtgactgtg tcacgctgtc cactgacctg ttctccctcc tgttcaacgg ataccggcac
     4021 aaaggcacca aacacccgca aaagctctga agtatcaagc attttcgcat tccgttcacc
     4081 ccgacaggtg gacagttcac cggctttaat taacctgtgc agggttgtcc tgctcttacc
     4141 tgtcaggcgt gcagcctcac tgatcgacac tttcgccatc agtcaccctc ctgtagtgca
     4201 tccagtctga gggacagtgt acggaaaaga tgctccgcat catcgtggag tttctcacaa
     4261 aggtcagcct ccgcatcgag atcaagctcg ttaagcttct ccagcaggag cagggactga
     4321 ttctggatga atttagccat gttcagggca gttttctgtt gtttcgtcat aaggggcctc
     4381 agggcgtcag tgctcagtgt aaaaccggtc tcttttccgc ttcctcgctc actcagtcgc
     4441 tacgctcctg ccgttcggct gcggcgagcg gacgggacac acagaagagt catcgcgata
     4501 accgggaaga aaagagcctg tggaaaaagc cacggcgttg ccgtttttcc ataggctccg
     4561 cccccctgac aagcatcaca gaaacggacg ctcaaatcag cggtggcgaa acccgacagg
     4621 acttaaagat accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc
     4681 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tatctcattc cacgcctgac
     4741 actcagttcc gggaaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     4801 gtccgactgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc
     4861 aaaagcgcca ctggcagcag ccactggtaa ctgatttaga ggagatggac ttgaagtcat
     4921 gtgccagtta aggctaaact aaacgaacag ttttggtgac tgcgctcctc tatcttagcc
     4981 agttacctcg gttcagaaac ttgccggttg ctgaaccttc gaaaaaacca ccttgccggg
     5041 tggttttttc gtttacagag caagagatta cgatgccaaa gaaaggatct caagaggatc
     5101 ctgttcaata taccttcccc tgaagggaaa ccagcgacgc cagtgaacta acagaaaaca
     5161 aaagcatttg atcttattaa aaaaaatgaa tatagagtaa gattttacac aatcagtggc
     5221 tttgttgaat aaatcgaaat tttggtaacc tgaagaatca gatcacgcat tatcctgact
     5281 atgcaggcag ttccgtggca aagcagaaat tcaaaatcac caactgacgc aactacaaca
     5341 aggccctgat taatcgtgac tccatcactt tctgggttga tgatgaggcg actctggcct
     5401 ggtatgagtc agcaacgcct gcatcgcggg gacaacctca gcgctataca gcccctgcca
     5461 tcacaacctt cctggtgatt aaacgtgtat tccggctgac cctgcgaacc gctcagggct
     5521 ttattgattc catttttgcc ctgatggatg tcccgctgcg ctgtccggat tacaccagtg
     5581 tcagcaagcg ggcaaagtcg gttaacgtca gtttcaaacg cccacccggg gtgaaatttc
     5641 gcatctggtg attgattcca cagggctgaa agccttcggt gaaggtgagt ggaaagtcaa
     5701 aaagcacggc aaagagcgcc gtcgtatctg gcgaaaactg catctggctg ttgacgcgag
     5761 caggcatgaa gccatctgct cggacctgtc actgaacaac gtcacggacg ctgaagcttt
     5821 cccggggctt atccggcaga cccaccgaaa aatcaggtca gcggcggcgg acggcgctta
     5881 cgatacgcag cgatgtcatg acgaactgcg tcgcaagaaa atcagcgcgc tcattcctcc
     5941 ccgaaaggga gcgggctact ggccgggtgt gggaacagcc ctgcgaaacc ttgcagcagc
     6001 gttcacgcat tacaatgaaa accacccgcc cagcgcgctg ggatatcact ctccgagaga
     6061 ataccggcaa ctgcgggcat cgttaactta agatacaaaa gctgtccgga gatggcgggt
     6121 caagatcact caggccgaga tccgccgtct ccagaaagag ctgaaacggg ttaccgatga
     6181 acgggacata ttaaaaaaag ccgcggcgta cttcgcaaaa ctgtccgact aaggtacgcc
     6241 tttatccgtg acaactcctg ttgctggcct gttcgcctgc tctgtcgggt gctggatgtt
     6301 catcccagtg gtttttacgc ctggcttcag cagccgcatt cacaacgcca tcaggcagac
     6361 ctgagactga caggacagat taaacagttc tggctggaat cgggatgcgt ctatggttat
     6421 cgcaaaatcc atctggatct gcgtgacagc gggcaacagt gcggaataaa cagagtctgg
     6481 agactgatga aacgtgtcgg aataaaggct caggtcggat accgaagccc gcgggcacgt
     6541 aaaggcgagg ccagtatcgt gtcacccaac aggctccagc gacagttcaa tccggatgct
     6601 cctgatgagc gttgggtaac ggacataacc tacatcagga cccacgaagg ctggctgtat
     6661 cttgccgttg ttgttgatct gttctcacgc aaaattatcg gctggtccat gcaatcccgg
     6721 atgacaaagg acattgtcct gaacgcactg ctgatggctg tatggcggcg taatcccgaa
     6781 aaacaggtgc tggttcattc ggatcagggc agtcagtaca caagccatga gtggcagtcg
     6841 ttcctgaaat cacacggcct ggagggtagc atgagccgtc gcggtaactg ccatgataat
     6901 gcggttgcag aaagtttttt ccagttgttg aaacgtgaac ggataaagaa aaagatctac
     6961 ggaacgcgag aagaagcccg cagtgatatt tttgattaca tcgaaatgtt ttataacagt
     7021 aagcgtcggc atggttctag cgaacagatg tcaccgacag aatatgaaaa acagtattat
     7081 caacggctcg gaagtgtcta gattatctgt ggcgattcag tccaaccctt acggacacga
     7141 tacatcgcca ctggtaacgg gtgtacaaga tttactgact cagagttctt gaagttaaag
     7201 ccttactgcg gctaaactgt aaggacaaat taggttgcag cggtcttgta caccctgtta
     7261 ccacggttca gaagttcccc aacttactga cccatcgaaa aaaccaccac cccaggtggt
     7321 tttttctttt ctgctactta gattacgatg cagtgactcc aatctcaagg aaaaacgatc
     7381 aggtttcgaa agtaggtaag aacatcatca aaagatcacc ttgcttgtaa aaagtacctt
     7441 cagccaaagg cgataatcaa gaagaaatag cgcatgcaac ttgcaaatac cacgagcgta
     7501 gtgcatgaat taatattcat cctcccacca ctcagccatt tgtttttcca aggcaggtcc
     7561 aaattcattt ccaattccag cccctggagt aaatgtaatc tcaccaaaat atattcggtc
     7621 acctaaaata taccaatcaa tacgtacgta attaaaatca ttaaggataa gtcttttatt
     7681 taataaaatc acttcattta aaacagcaac ctcattatta cttaataaat gattgccagc
     7741 gccatgactc caaaccatat tttcaaaaga atttaaaaaa tagcttcgtg actcctgccc
     7801 aaagcgaccg tccactaact gcaacacaaa attaatatca ccattttttt gtttgaaaca
     7861 atgaaacttg taatcacgag ggatttcacc acgttttgtt attttcctct ccactaaaag
     7921 cattggcttt atcttggagt aatgcatttc atcgcaaaaa gaggaaaagt cagttttcaa
     7981 ccaggttttc gcagtggtaa caacattgtc tttctcctct ttagagggat tgtcatcgga
     8041 tatataaatc atgcctgccg catgatttgg tttgataacc aatccagacc aatcatctat
     8101 ttttaataag tcactagggt tgctagtagc caaatggaga ggaataagat actcctctcc
     8161 aacccgctcg gatacgaaac gccgcacaag atatttatca gccaatgtgg agtatatatc
     8221 cttttgttct cctagtattc tactaataac cttttcatta aaggttttag gtttttttat
     8281 atttggaagc tttttataca ctctaaaata tcttagttta actctattgg ttgtagacaa
     8341 attctccaaa agcaacctgt aagccttacc aaaaaaaaac ttaatcactt caattctctc
     8401 tttttaatct gttaaatcag aaagcatgta tactcgagac actccaagtt tcatgcgcct
     8461 tggcttcttg atgcactgca gataagggtt ccacggaact cagtctacac actcaatctt
     8521 attaaacata cagataaata ataaaaaatc ataagtaaca ataaaattca aaaactcacc
     8581 cccaacacac cagaactaat catcatattt taaatttcat aagaaaatca ataaaacaca
     8641 atccataaat aaatctaccc ccccaccatc aacgcctcat caacccaata acatttaaat
     8701 gaatccttag aatcattcca ttcctaaaga aataatttaa taatgtgcat gacagaactt
     8761 cgaccacaag aaaacaatac gcagcaccga aaacatcata acggttaatc ataacatatg
     8821 agaatgggat gctgagcaag ctagttaacg tcattttata tgaaaggtat ttgtagccag
     8881 agtattttat tatgaatcta taacaaatag ttcccaagga tgaaaacatt gtcgctagaa
     8941 taatgattgg caccaactca acagccagtt tataggccga accatagagc atgctgataa
     9001 tgtacggccc aagaaaataa aaaccaaata atataaaaat tgttatcaag atcacaattc
     9061 tatttattaa agttagaagc ctagacattt tttcaaaatc tttctcttga tagattttag
     9121 aaaaaaaact agtgattaac gcaactggaa taaatgaata cgcactgcca agcgtcagcg
     9181 ccacattata aatacccaaa tcagaatatg aggttatttt agccaaaaaa atatttgata
     9241 cctgtgtgta aatcacaatg gacagggttg atagtaacaa cgaccctccc gctgaaaaca
     9301 aatacttatt atatttcttc gttttcgatt ttaataccgc agtgtcttca atggtattgt
     9361 gaaagtatat atatctaata acataaggta ttgcagggat taatataata ggaatgctaa
     9421 atgtatatac tggcaactcc aagtatgaaa ttaaaaagcg gactataaaa gcaatacaca
     9481 accctgcacc atttgttaat gcattaattc tagatattaa ctgcgtatta ttatatacag
     9541 cgaaaacatc catcactata taataagtgg caatgcagtt agccacccca aaaacaaatg
     9601 tcagggtatc tgtaaaaaaa aacaaataga acaaggcaat caatgaagag agtgcaaaaa
     9661 taaccctgcg ctgcttttga gtatgtaatg caagagcaat acctgatttt cttttctcac
     9721 tcatcctttt aaaaagtatg ttttgcccac caaaccaagc catggtctgt acaaacgtaa
     9781 atattgtcgt agcaaaagta attttaccaa aatcagtagg tcctatatat tttgccatat
     9841 aggagtttat aaacacaaga ccgatgatgc tgactaattt ttcagccatc atccaaaagg
     9901 cattagaata cattactcac tcgcaaaaaa ttaatcccat agaaattttg gtaatgctgt
     9961 caacttgttg ataactattt taatctgtag tcattcaatc cttgtaacat caggataatc
    10021 ctagtgtcag tgaaaatacg aaatcaacaa gttggtatca ttaccagcgt cggcatggtt
    10081 ccagcgatca gatgtcaccg acagaatatg aaaaccagta ttatcaacgg ctcggaagtg
    10141 tctagattat ccgtggcgat tcaaaagctg atttttgtcg tggtctaact actgcaaaga
    10201 atccacctaa aagctctctt ttgacatatc ttctacatct tataaattga ataaaaccta
    10261 taaaaaacaa caatttaggc acgctaccgc cctaggctct tcagctacct atccactgca
    10321 ctaacgctaa cgactatctt taacatttgg gttgaggtca aatcattaag taacttaaca
    10381 ataaacacga taacttcagg aaggaccgat ttcacccttg cttatgatgg cgtaacgttt
    10441 tcttatcatg gaactttcac gagacttgcc attttctgca aattccgatt cctttacact
    10501 tatcagtata ctgactcagt ggactaacat cgccgttgat gcaattacag aattaacaaa
    10561 aaagtggtgg ccaccgctgc gaatgtacat gacctgaatc aggttgaaaa cctgatcaca
    10621 gagcctatgg aacacataat ttaaggtaat gctgccaact tactaattta gtgtatgatg
    10681 gtgattttaa ggtgcttgcg tggcttccat ttccatcaga tgtccttcct gctccgctac
    10741 tgaaggcgtg gtgcgtaacg gcaaaagcac tgccggacat cagcgctatc tctgctctca
    10801 ttgccgtaaa acatggcaac tacagttcac ttacaccgcc tctcagcccg gtacgcacca
    10861 gaaaatcatt gatatggcca tgaatggcgt cggatgtcgc gccagtgcac gcattatggg
    10921 cgttggcctc aacacgattt taagacactt aaaaaactca ggccgcagtc ggtaacctcg
    10981 cgcatacaac cgggcagtga tgtgattgtc tgcgctgaaa tggacgaaca gtggggctac
    11041 gtcggtgcta aatcacgtca gcgctggctg ttttacgcgt atgacaggat acggaggacg
    11101 gttgtggcgc acgtcttcgg tgaacgcact ctggccacac tggag
//
