LOCUS       NZ_CP074491             4082 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Escherichia coli strain S7_CRE7 plasmid pCRE7_5, complete sequence.
ACCESSION   NZ_CP074491
VERSION     NZ_CP074491.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387627
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Characterization Of Recent Carbapenem-Resistant Enterobacteriaceae
            Isolates From A Large Tertiary Care US Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2021) Biological Sciences, University of Alabama
            in Huntsville, 301 Sparkman Dr, Huntsville, AL 35899, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 347x
            Sequencing Technology        :: Illumina NextSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 02/27/2024 01:29:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074491.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S7_CRE7"
                     /isolation_source="abdominal fluid JP drain"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pCRE7_5"
                     /geo_loc_name="USA: Durham, NC"
                     /lat_lon="36.0075 N 78.9384 W"
                     /collection_date="2017-02-07"
                     /collected_by="Rachel Addison"
     gene            complement(join(2313..4082,1..390))
                     /gene="mobQ"
                     /locus_tag="KHV93_RS00005"
                     /old_locus_tag="KHV93_25240"
     CDS             complement(join(2313..4082,1..390))
                     /gene="mobQ"
                     /locus_tag="KHV93_RS00005"
                     /old_locus_tag="KHV93_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            569..823
                     /gene="mobC"
                     /locus_tag="KHV93_RS00010"
                     /old_locus_tag="KHV93_25245"
     CDS             569..823
                     /gene="mobC"
                     /locus_tag="KHV93_RS00010"
                     /old_locus_tag="KHV93_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1356..2057
                     /locus_tag="KHV93_RS00015"
                     /old_locus_tag="KHV93_25250"
     CDS             1356..2057
                     /locus_tag="KHV93_RS00015"
                     /old_locus_tag="KHV93_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(33..391)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          392..568
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1357..2057
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2317..4082)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgttttatcc atgtgatgga acgccacatc agcaatcatg ccctgactga caaagttttc
       61 tctgacaaag gcgagaacgg tttcccgtgc ggcatcctgc ggtagttcgc gggggatggc
      121 gagttcaatt tcccgcgcaa gttgtgagtt cttccggcgt tcggccttct caacggcatt
      181 ccagagttcg gcacggttcg tacaccaggc aggggctgtc gcgggagtca gaatcacagc
      241 ttcagccaca ccgcttttgc gggtgtagtc gtgagtcagt ccggtacgct catcaagcat
      301 cagtgaaccg gaacgatagg cagcagaagc aacggcgctg tagccgctgt ttctggagat
      361 gattttcatg ctgagatgat aaatcgccat ttctgccgta atgccttttt gtggttttct
      421 ttcagccccg cagggcgcac acaatcgaaa cggagtggag attgtataag tgcgcacttc
      481 gtgattttcc gtgcattatg ccagctcagt ttttaaacta ccagtgacgc gctaaaatgc
      541 gatttttcat cgcgcacgga gttacatcat gagcagaaca ctcgaacaga agattgctga
      601 tgctgaggcc agattacagc gcctgaaggc gaagagcagg agtctggaca cagcgcagaa
      661 ggtcattgtg ggtgcagcat tgctggcaaa ggtcagaaag ccggaggagg tgcagttgcg
      721 tgcctggtta ctccagttcc tgaaggcaga ggtgacacga caggctgatg tgacgcgcat
      781 actgccgctg attaacgagc tggaagcgct gccaggacag tgattgtatc gtccgctcgc
      841 cgcagtccca gcgaccgagc gttagcgagc gagtgggcga ggaagcggaa gtgaatccgt
      901 cgcctgattg agcacatacg cacggttttt cgggaggtga tatgcgctga acccgcatga
      961 atactggatt tccacagggt tggagggggt gtattcggaa cttgcaattc tgcttttttg
     1021 caacgcattg tttttattgg ttttttgttt tcagtactcc aagttatcca caggctcgtc
     1081 gttaagtttt tagatcttgt ttttaagatc ttgtttttaa tacttgtttt agcgccaaaa
     1141 ttccttatag aaaacaaaat gttacaatgc tataggtaat gtgatttaag gtaaaggtaa
     1201 tatgatttaa ggtgtttagg tgggggaatt taaggtaaag gcaatatgat ttaaggtaat
     1261 attcacagaa aaagggatat taaaataaaa aggcattgcc tttcgttgag ggtatgttta
     1321 tcttttattc attacctttt ggggagggat gctctatgtc ggatctaatt gcctataaat
     1381 ccaatgcgct ggttgaagcc agttataaat tgaccctaca ggaacagcga tttttgttac
     1441 tttgcatcgg tcgattgaag tctggggctg atgcagaatc tccaaaattg caaaagacaa
     1501 tgaccattac agctgcggaa tatttcgatt cattccctga catggggcgg aaaaatgccg
     1561 aggttcagtt acaggaagca attgatcgtt tgtgggacag gtcaatcatt ctcaaggatg
     1621 acgagaaacg tgaggagttc cgctggatcc agtatcgggc acagtacgct aaaggcgagg
     1681 ccagagcaca aataacattt tctgacgcag taatgcctta tttgacacaa ctacaagggc
     1741 aatttactag agttgtaatc aagaatatat ctagattatc aagaagttac agtataagga
     1801 tatacgaact cctccagcag ttccgcagta ctggcgaacg tattatcgct ctcgatgatt
     1861 ttcgctcaat gcttgatata gagcataaat atcaaacata taaatcactt aaccagcaat
     1921 tattaagacc ctgcattgac gaactgaaca aaaaaagcga cctggctgta acggttgaga
     1981 cgatcaaaaa ggggcgcacg gttgtagccc tgcattttcg cttcaaggaa gacaaacaaa
     2041 tcaagatgac gatctaaaat cgattttaag gcacctaaaa cgctccctgc tgcgtttttt
     2101 ttgtgttagt agtgcgaagg tactattgaa gtgaaaaatt gcgtcaaaag cgctctaatg
     2161 cagcgaaaaa agaaccccca ttttttgggg gcggaaaaac aaaactatgc ttgcattatg
     2221 tgtgtgcttc ttgcattcat ccctgtcgat gaacatcgaa gaacatcgac gaacataaca
     2281 cgatgttaaa caaaacaaaa tattgttcac attcactgtg accaaccgcg atcatgtctc
     2341 ctctcggtgg cgtgtgttcg tgcatcttcc tcgcgccgcc attcgtccca ggtacgatta
     2401 aaacccagcg agcggaacag atgatccggc tgtttttctg gtgtgttgac ggcagcaaaa
     2461 agcggtgcct cacgggtttt cagatgaaac tccataagtt ccggcgcagg tgatatgtgg
     2521 ctgtctgcat cgtgatttac ggcaacacca taccctgcat accgtaaccc cttatcggtc
     2581 agtgcactga tgaccagctg gctgttatcg gctgagggaa cggcaaacgc gggatacagc
     2641 gtaccattaa gccggacaac accgcccaga gagtcatcca cgcccatgac cagcaaaagc
     2701 tgaagctggc gggtttcccg gtcgacagaa ctcattaccg gcatcagcgt cggtagcaga
     2761 cgtccctgcg taaaatccac cttccttgct gccagtgatg gcatggctgg ctggcggtct
     2821 tctccaagac agaaagactc cggccatgtc ttcggctggc ggagattgcg ggtatattgc
     2881 ggccatgtct ggcaaacctg cgtcagaagt tgctcaaagg tcgcagagga ataaacacgt
     2941 tgcagtggtg gatgttgacc gatttcgctt cggtcacaga tataaccact cctgtcacgc
     3001 attaaatcaa cacgctcaaa gattctggtg cggtatttct ccagctcgtt cccccagagc
     3061 gtgtattctc cgttccggtt gtgaagcaga aggtaacagg tcatttcccc gttaccaggc
     3121 atgggggcct cgccataccc cgtaacgact gcgctcagta cccctgaatc cataacggca
     3181 ttgtcatttt tcctgagctg ttccgctcgt cgctccgcgc gactacgttc tgtatcctcc
     3241 tgctccttcc ggcgcaattc ctcttcacgt ttaagcaact ggactctgag agctgcaagg
     3301 ccggatgccc cccactgctg ccactgtttt tcaaatgccg tctgactcgc gggaatgtcc
     3361 tgtttctcca ccttccggta atgctgtcgc aatttttcca tttgctggct cagcgactgg
     3421 atttttccct gttcgctacg gtattccggt gccgcccatt gcatcagggg aatacgggaa
     3481 aaaccggacg aaaacggcga tgattttttc aggctggcag catggctcat cgcttccttt
     3541 cgttcggtgt tcagctcttc ccagcgtctg cgcgtcgcct gcatatctgc catcatcgac
     3601 atggcattac tggtgagttt ccatgccgca ttactgtcct tcggcaacgc ttcgatgatg
     3661 tcacgtagtg tgtacgaagt tgcgttgtca ggaagcacga tattcagaag gtcagccaca
     3721 cggcgggcag gctctgtcag cccgctgttt ctgagttcgg tacgggaaat ctgtatttcc
     3781 aggttaagtc tgttaatcag acgattctgt tcgccgcgtt ctgtttcgat accgcgtgtt
     3841 tccatcgcac aggcagactt tccgaggtga ataccaggcg ttttctcaag cccctgtcgc
     3901 tcgtatgaac gatggtctat ctcttcctga taaccggcgt tcgccagtgc cctgttcgca
     3961 tggtcagccc atgatgcacg ccacgtctcg gcatgtgtcc ggtcgttcca gtccctgacc
     4021 tttccaccga atccagcagg tccgacagct ctcgtggtca gcatgatgtg cgcatgggga
     4081 tt
//
