LOCUS       NZ_CP074490             5153 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Escherichia coli strain S7_CRE7 plasmid pCRE7_4, complete sequence.
ACCESSION   NZ_CP074490
VERSION     NZ_CP074490.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387627
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Characterization Of Recent Carbapenem-Resistant Enterobacteriaceae
            Isolates From A Large Tertiary Care US Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2021) Biological Sciences, University of Alabama
            in Huntsville, 301 Sparkman Dr, Huntsville, AL 35899, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 347x
            Sequencing Technology        :: Illumina NextSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 02/27/2024 01:23:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074490.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S7_CRE7"
                     /isolation_source="abdominal fluid JP drain"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pCRE7_4"
                     /geo_loc_name="USA: Durham, NC"
                     /lat_lon="36.0075 N 78.9384 W"
                     /collection_date="2017-02-07"
                     /collected_by="Rachel Addison"
     gene            50..187
                     /locus_tag="KHV93_RS00005"
                     /old_locus_tag="KHV93_25210"
     CDS             50..187
                     /locus_tag="KHV93_RS00005"
                     /old_locus_tag="KHV93_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001329514.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            746..901
                     /locus_tag="KHV93_RS00010"
     CDS             746..901
                     /locus_tag="KHV93_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001490772.1"
                     /translation="MSVDFCDARQGGGAYGKTPAALASSGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            complement(1029..1223)
                     /locus_tag="KHV93_RS00015"
                     /old_locus_tag="KHV93_25215"
     CDS             complement(1029..1223)
                     /locus_tag="KHV93_RS00015"
                     /old_locus_tag="KHV93_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041621.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1507..1830
                     /gene="mobA"
                     /locus_tag="KHV93_RS00020"
                     /old_locus_tag="KHV93_25220"
     CDS             1507..1830
                     /gene="mobA"
                     /locus_tag="KHV93_RS00020"
                     /old_locus_tag="KHV93_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(2055..2474)
                     /locus_tag="KHV93_RS00025"
                     /old_locus_tag="KHV93_25225"
     CDS             complement(2055..2474)
                     /locus_tag="KHV93_RS00025"
                     /old_locus_tag="KHV93_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2555..2992)
                     /locus_tag="KHV93_RS00030"
                     /old_locus_tag="KHV93_25230"
     CDS             complement(2555..2992)
                     /locus_tag="KHV93_RS00030"
                     /old_locus_tag="KHV93_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001318234.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            3252..4817
                     /locus_tag="KHV93_RS00035"
                     /old_locus_tag="KHV93_25235"
     CDS             3252..4817
                     /locus_tag="KHV93_RS00035"
                     /old_locus_tag="KHV93_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447003.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     Typing          1259..1498
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(471..1000)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.59"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1508..1827
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.11e-62"
ORIGIN
        1 ataaattaaa cctcgttata tattcttttc attttataag gatcgagtta tgaggaaaag
       61 attttttgtg ggaatattcg ctataaacct ccttgttgga tgtcaggcta actatatacg
      121 tgatgttcag ggagggactg tggctccatc atcttcttct aaattgacgg ggatctctgt
      181 tcagtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      241 ctgtaaacga aaaaaccgcc tggggaggcg gtttgatcga aggttaagtc agttggggaa
      301 ctgcttaacc gaggtaactg gcttcagcag agcgcagata ccaaatactg tccttccagt
      361 gtagccgtag tcgggccact acttcaagaa ctctgttgca ccatttgcgc catcatcgct
      421 ctgctaacca agttaccaac ggctgctgcc agtggcgcta agtcgtgtct taccgggttg
      481 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      541 acacagccca gcttggagcg aacgacctac accgagccga gataccaaca gcgtgagcta
      601 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      661 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      721 cctgtcgggt ttcgccacct ctggcttgag cgtcgatttt tgtgatgctc gtcagggggg
      781 cggagcctat ggaaaaacgc ctgcggcgct ggcttcttcc ggtgctttgc tttttgctca
      841 catgttcttt ccggctttat cccctgattc tgtggataac cgtattaccg cttttgagtg
      901 agctgacacc gctcgccgca gtcgaacgtc cgagcgtagc gagtcagtga gcgaggaagc
      961 ggaagagcgc cttatgtgac attttctcct tacgctctgt tgtgccgttc ggcatcctgt
     1021 cctgagcgtt atctctctgt gctattttct acttcaaagc gtgtctggat gctgttctgg
     1081 agttcttctg cgagttcgtg cagcttctca cacatggtag cctgttcgtc agcatcgagt
     1141 gcgtccagct tttcgagcag cgtcaggctt tggcttttta tgaatcctgc catgttgagt
     1201 acggcttgct gctgcttgtt catcttttcg ttttctccgt tctgtctgtc atctgcgttg
     1261 tgtgattata tcgcgtacca cttttcgact gttttaccgc cggtattctg ccgtctgacg
     1321 ctttgacggg tatttctgcc tgacaatact gtcactgcca aaaaactgcc gtgcctttgt
     1381 cggtaattcg aacttgctga caggacagga tgtgtaattg ttataccgcg catacatgca
     1441 cgctattaca attaccctgg tcagggcttt gccccgacac ccatgtcaga tacggagcca
     1501 tgttttatga caaaacgaag tggaagtaat acgcgcaggc gggctatcag tcgccctgtt
     1561 cgtctgacgg cagaagaaga ccaggaaatc agaaaaaggg ctgctgaatg cggcaagacc
     1621 gtttccggtt ttttacgggc ggcagctctc ggtaagaaag ttaactcact gactgatgat
     1681 cgggtactga aagaagttat gagactgggg gcgttacaga aaaaactctt tatcgacggc
     1741 aagcgtgtcg gggacaggga gtatgcggag gtgctgatcg ctattacgga gtatcaccgt
     1801 gccctgttat ccaggcttat ggcagattag cttcccggag agaaaactgt cgaaaactga
     1861 cggtatgaac accgtaagct cccaaagtga tcaccattcg ctttcatgca tagctatgca
     1921 gagagctgaa aacgatcctg acgcatcctt cctgtttttc cggggtaaaa catctctttt
     1981 tgcggtgtct gcgtcagaat cgcgttcagc gcgtttcagt ggtgcgtaca attaagggat
     2041 tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc agaaatcgtg
     2101 tcagccagca gcctggattt tttcggggta aggtctttgc tgcggtaatc agtacccgca
     2161 aattgagttg tgccccggag tgagtttaaa aattcctggc tgtgaaactg ttgaatgcgt
     2221 tgttgcacat cactccgtga ttttcctgtc gctttatctt tagctcccat atcccagaac
     2281 tgccgaaaaa aaggcaccat cattcgtgtt gtgtcggctt tttgggtgga aaagttggga
     2341 tcaaaaccgt catcagaagc atatacgtaa tgccgggtat gttgttccac gctgcccttt
     2401 ggggtacagg ctgtgagagc tactgttgct gttatcatgg cgataagaca aagtttgttt
     2461 ccggtacgtc tcatgagtgt ctccctacct taaagtattt attctcggtg agttattatc
     2521 cgaagattat cttatcatca gtttctgagc gcgttcagtg accgaacagc ctggaccaga
     2581 atccccgttt cttttcctgc tccagagcct gcctgagctg ggctatctca ttctgtagct
     2641 gtttccgttc tgcttcctgg cgccggcgct ccgtatcctg ggcttgttta tcctcaagca
     2701 tcagcgtcag gcattgtttc agctcattca gttcccggag aatgcgttct gtctgctgat
     2761 catgaggttt ttctgagtgt ccttgagtgt gcctttctgg tgtctcattc cgctttaatt
     2821 cgccgtatgc ccggatcagt tcactggttt caaactcccg tcgtccgtcc tggctggtac
     2881 ggtaactgac aagacctgag cgcatatcac ggtaaaactg tgaacgcgat cttccagtca
     2941 ttttcagtgc gtcccggagt gtgtgccatg ccataaagtt acagtgtccc ataggtgtct
     3001 catctcatgg tttcagtaaa acataatgag gtctgagaac ggtaatgttt gtgctggttt
     3061 ttgtggcatc gggtggaaat agcacgtggt gtgggtggct gtttttttga tcgttttcac
     3121 aaaaatgaaa atccacaggg ttgacaggga aaatgcagcg gcgtaatttt aaactgtata
     3181 taaaaccagt gattatatat acagtatatt gcttttaact atttgttttt aatgtcaaag
     3241 aggattttat aatggaaaca gctgtagcgt actataaaga tggtgttcct tatgatgata
     3301 aggggcaggt aatcattact cttttgaatg gtaatccaga cgggagtggc tctggcggtg
     3361 gtggtggaac tggaggtagc aaaagtgaaa gttctgcagc cattcatgcc acagctaaat
     3421 ggtctactgc tcaattgaag aaaacgcagg cagaacaggc tgcccgagca aaagctgccg
     3481 cagaagcaca ggctaaagca aaagcaaacc gggatgcgct gactcaacat ctgaaggata
     3541 ttgtgaatga ggcgcttcgc cataattcca ctcatccgga ggttattgac ctggctcatg
     3601 ccaataatgc agcgatgcag gcagaagcag agcggttgcg ccttgcaaaa gcagaagaaa
     3661 aagcccgtaa agaagcggaa gctgcggaaa aggcttttca ggaagcagaa caacgacgta
     3721 aagagataga gaaggagcag gctgaaacag aacgccaatt gaaactggct gaggatgaag
     3781 agaaacgcct ggcagcattg agtgaagagg cccgggctgt ggaggtggca caaaaaaatc
     3841 ttgctgctgc acaatctgag ctggcgaaag tggatgaaga gattaatacg ctcaataccc
     3901 gtttaagctc cagtattcat gcccgtgatg cagaaacgaa tacgctgtcc ggaaaacgaa
     3961 atgagttgga tcaggcatct gctaaatata aagaactgga tgaaagggtt aaacttttat
     4021 ctcctagggc gaatgatccg cttcagagtc gtcctttttt tgaggccacc agactacggg
     4081 cgagagctgg tgatgagatg gaggaaaaac aaaagcaggt aacagcatca gaaacgcgtc
     4141 ttaaccagat tagctctgag ataaatggaa tccaagaggc tatttctcag gctaataata
     4201 agcgaagtac agcagtttca cgtattcatg atgctgaaga taatttgaaa acagcacaga
     4261 ctaatctcct gaactcgcag attaaggatg ctgtggatgc aacagttagc ttttatcaaa
     4321 cgctatctga aaaatatggt gaaaaatatt caaaaatggc acaggaactt gctgataaat
     4381 ctaaaggtaa gaaaatcagc aatgtgaatg aagctctagc tgcttttgag aaatacaagg
     4441 atgtattaaa taagaaattc agcaaagcag accgtgatgc aattttcaat gcactggagg
     4501 cggttaagta tgaagactgg gctaagcatt tagatcagtt tgccaagtac ttgaagatta
     4561 cgggacatgt ttcttttgga tatgatgtgg tatctgatat cctaaaaatt aaggatacag
     4621 gtgactggaa gccgctattt cttacattag agaagaaagc tgtagatgct ggagttagtt
     4681 atgttgttgt tttacttttt agtgtgcttg ctggaactac attaggtatc tgggggattg
     4741 ctattgttac aggcattcta tgtgccttta ttgataagaa taaacttaat actataaatg
     4801 aggtgttggg tatttaatta ttctttattg atataaacag tcctagcaat ccaaatggga
     4861 ttgctaggac caaacaaagt agattgtata gcataaatag gtttaatttt gccacggggg
     4921 cattatttag gttttttctt tttctgaaaa aatctttctt tatgaactta aaaaatatgt
     4981 gttcaatagc atattttgaa tatgggcata gaatagtgct tatcactatt gcgtatagca
     5041 tcttatctga cgctaggaaa taataccctt cgttgttttt tgttataagg tatatatata
     5101 taagtgcgca gtataggcca aacaaaatat tttttatgta gtatcttaaa ctc
//
