LOCUS       NZ_CP074488             5701 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Escherichia coli strain S7_CRE7 plasmid pCRE7_2, complete sequence.
ACCESSION   NZ_CP074488
VERSION     NZ_CP074488.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16387627
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Characterization Of Recent Carbapenem-Resistant Enterobacteriaceae
            Isolates From A Large Tertiary Care US Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2021) Biological Sciences, University of Alabama
            in Huntsville, 301 Sparkman Dr, Huntsville, AL 35899, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. v0.4.7
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 347x
            Sequencing Technology        :: Illumina NextSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 02/27/2024 01:25:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074488.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S7_CRE7"
                     /isolation_source="abdominal fluid JP drain"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pCRE7_2"
                     /geo_loc_name="USA: Durham, NC"
                     /lat_lon="36.0075 N 78.9384 W"
                     /collection_date="2017-02-07"
                     /collected_by="Rachel Addison"
     gene            1..906
                     /locus_tag="KHV93_RS00005"
                     /old_locus_tag="KHV93_25150"
     CDS             1..906
                     /locus_tag="KHV93_RS00005"
                     /old_locus_tag="KHV93_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015058918.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFTYGVRILPKDIAILARFIQQNQP
                     HAQHWLVFDVDRTGAAIDWSDRNCPAPNITVKNPRNGHAHLLYALTLPVRTAPDASASA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHQEWQVVEWRLELYTLDELADYLDLSASA
                     RRRVDKDYGMGRNWYLFEKSRKWSYRAIRQGWPAFSQWLDAVIQRVEMYNASLLVPLSP
                     AECRAIGKSIAKYTYRKFSPEGFSAVQAARGRKGGKIGGAKSKRVAVPTSARSLKPWEA
                     LGISRATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(1111..1557)
                     /locus_tag="KHV93_RS00010"
                     /old_locus_tag="KHV93_25155"
     CDS             complement(1111..1557)
                     /locus_tag="KHV93_RS00010"
                     /old_locus_tag="KHV93_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_170889589.1"
                     /translation="MTNLEEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKSAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFNAAELVGALAGVAELPRNHPKWQEWEKRGKELLTKDGA"
                     /name="conjugal transfer protein TraD"
     gene            1740..2825
                     /locus_tag="KHV93_RS00015"
                     /old_locus_tag="KHV93_25160"
     CDS             1740..2825
                     /locus_tag="KHV93_RS00015"
                     /old_locus_tag="KHV93_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_218791192.1"
                     /translation="MASYHCTVKTGAKGVALKHADYISRSGEYKNYKSREDLEFLSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKARF
                     GETPQERKSALVALRSHWADVQNEHLARHGHQSRVDHRSLKEQGIKRTPEVHLGPVRVA
                     VMNADQVCAILARRNAEREKLEAEQERDSIIDLKTTLKEALAERDNIPPSLDELGAELK
                     AFVESSFDELNEVMQLNSLINGALDSMMKQFQVEEERKLQQERLIEAEKQRDLERERER
                     VRQRAYSSPAPSRSRDYGMSR"
                     /name="MobA/MobL family protein"
     gene            2846..3562
                     /locus_tag="KHV93_RS00020"
                     /old_locus_tag="KHV93_25165"
     CDS             2846..3562
                     /locus_tag="KHV93_RS00020"
                     /old_locus_tag="KHV93_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001175444.1"
                     /translation="MQLDEHSRKIAALVAYNDKQIKEMNRLITLLNERVNDLNTFTSTL
                     EREGKVVSGISYSFRDSVSNVKFYINKGMDEASCKLQKSVSDTLTSLLREKLSFTLNDV
                     KKEWVDFEEQTSKEIKESLKQTTREAEVATGIIRKTMEKYTVTRIVEKVIVLIVLFLLL
                     MIAPVFYAEYLKMNISSLMEEQKKHESMLKEWKEKAPLAEFSKCGDYRKACVLVDDESG
                     YYFNEQSPNKKYAILK"
                     /name="hypothetical protein"
     gene            complement(3620..4192)
                     /locus_tag="KHV93_RS00025"
                     /old_locus_tag="KHV93_25170"
     CDS             complement(3620..4192)
                     /locus_tag="KHV93_RS00025"
                     /old_locus_tag="KHV93_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="WP_001522465.1"
                     /translation="MEYSTLLSFAIVTLSQTISIGPGVALVINNAFLHGLKSSIKTSIY
                     IRIGETIVMAISLFALSSTSSTEQHFHIIKIFGGGYLIYIGLMGLINKKNKQRKEQKPF
                     LIPLLNPKAYLFFAALIPTFIDNNTNITLNFFILGVLFIFISFLTDLIYIAISLTIRDK
                     LTPSFSRYISICSSIFILGTGIYFIFT"
                     /name="LysE family translocator"
     gene            complement(4199..5149)
                     /locus_tag="KHV93_RS00030"
                     /old_locus_tag="KHV93_25175"
     CDS             complement(4199..5149)
                     /locus_tag="KHV93_RS00030"
                     /old_locus_tag="KHV93_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001058673.1"
                     /translation="MNNFINECISAPRINGMAEFFSSTASGEKHKPFRLVEIDDRLFHS
                     YDYIKFAKHLTKNSGPFVNHYFCSIPYSKEEECRLGTTVIKYAITLNKKLNTWCLGMAE
                     GAMARTISELSKGNIQTLTTSPTKENEHAFFKYGSSENAYFICTPFFLLEERLKNSNLK
                     QFNQGFDIIIEDTTFQMYSPNRSGQINHVKRLLNNDGIFIFTEKHSSEHDEYQKREIQK
                     DYSFKQRYFSKDEINTKKEIVLNTMNLNEVSVDDMLSAIKQHFKYASIYWNSGNFYSIA
                     ASNSLKHISTFINLLPKAAIPMEYVYEKLPRNISW"
                     /name="hypothetical protein"
     Typing          2..906
                     /name="rep_cluster_2141"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1741..2822
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.723"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg tacttcaacg cttcagggaa aaattaccgc acaaaccgta ctgtacgaac
       61 gatttcacgt acggcgttcg cattctgccg aaagatatcg ccatccttgc ccgtttcatc
      121 cagcagaacc agccacatgc gcagcactgg cttgtttttg acgtggaccg gacgggcgca
      181 gcgatagact ggagcgaccg gaactgtccg gccccgaata tcaccgtaaa aaatccccgt
      241 aacgggcacg cgcatctgct ctacgcactc actcttccgg tgagaaccgc gccggatgca
      301 tcggcttcgg cgctcaaata cgctgctgcg attgagcgtg cactgtgtga aaaattaggc
      361 gcggatgtga actacagcgg cctgatctgc aaaaatccgt tccatcagga atggcaggtt
      421 gtggaatggc gtctggaact ctataccctc gatgaactgg cggattatct cgatttgagc
      481 gcctcagcgc gccgtcgcgt cgataaagat tacgggatgg ggcgaaactg gtatctgttc
      541 gaaaagagcc gtaaatggtc ttacagggcg attcgtcagg gttggccagc attctcacag
      601 tggcttgatg ccgtgattca gcgtgtcgaa atgtacaacg catcgcttct cgttccgctt
      661 tcaccggctg aatgtcgggc tattggcaag agtattgcta aatacaccta ccgaaaattc
      721 tctcctgaag gtttttctgc ggtacaggct gcgcgtggtc gcaaaggtgg aaaaataggc
      781 ggtgctaaat ccaagcgtgt agcagttcct acatcagcgc gttctctgaa gccgtgggag
      841 gcattaggga tcagtcgagc tacgtactac cgaaaattaa aatgtgatcc agatctcgca
      901 aaatgagacc agataaagcc ttatcagata acagcgctct gttggcgttt ttttgagctg
      961 ttggttttgc tgtgtatttc tctgctcacc cccgcaatgg tgcggctttc cgcatgattg
     1021 aggtggtagt gctcgccgca gtctcatgac cgagcgtagc gagcgaatga gcgaggaagc
     1081 accagggtac cagaacgcga acggagactc ttacgcaccg tctttggtga gtagctcttt
     1141 cccgcgtttt tcccattcct gccattttgg atggttgcgt ggtagctctg ctactcctgc
     1201 tagtgcgcct accagttcgg cagcattaaa ttttggtttt ccggttttgc tgtccactag
     1261 tccagcaaca atcatgaggc ctgcggcttt gtagagttcg tgatcccgcg cctttcgttc
     1321 tgcccttgcg gcagacttcc gttctgccgc aatgattgct gttaccctag cttttgcagc
     1381 ggcggctttc tccgcggttt tttctgctcg tattagtgcg gataacgttt tttcatccgt
     1441 cgttgttcgg ttttttttct ctgccagtaa tacaagtaat cgctgctgct cagatggtga
     1501 tttcagaccg ttgatgtagg caatttttgc acttaaccac tcttcaagat ttgtcattct
     1561 tattcttatc tccgcacttt tacttttgcc gtttattgtt ttggtattgc gcataatatc
     1621 accttgtatg cccgagtacg tgaccgtaaa atcatccctg ataaactcag ggatgcgcac
     1681 ttagacgttt ctctccttcg tcgagaaaca gtgcgctcct gcggagggct taacggcaga
     1741 tggcaagcta ccattgtaca gttaaaactg gagcaaaagg cgtagcctta aaacatgctg
     1801 attatatttc tagatccggc gaatataaga actacaaatc gcgggaggat ctggaatttt
     1861 tgtcatccgg taacatgcca tcatgggcga agaaaaatcc cgcagagcta tggaaagcag
     1921 cagatgaatt tgaacgaaaa aacgggacgg cataccgtga aattgagatc gcattaccgc
     1981 gagaactgac acgcgaacaa cggatagaac ttgttgaaga tttcgtgcaa aaggagcttg
     2041 gtgatcgcca tgcctatcag tacgcgatcc acaatccgcc aggtgcaatc gacggaaagg
     2101 aacaaccaca cgcacatatc atgttttgcg agcggattaa tgatggtatt gaacgcgatc
     2161 cgcagcagtt ttttaaacgt gccaacagca aatcaccgga gcggggaggt gcaaaaaagg
     2221 cacgctttgg tgagacacca caagagcgaa aatcagcctt ggttgcgcta cgttctcact
     2281 gggctgatgt gcagaatgaa caccttgcgc ggcacggcca tcagtcgcgc gttgatcatc
     2341 gttcactgaa agaacagggt atcaagcgca caccggaagt tcatcttggt cccgttcgtg
     2401 ttgctgttat gaatgctgat caggtttgtg caattctggc acggagaaac gcggaacggg
     2461 agaagctaga agcagagcag gaacgggaca gcatcattga ccttaaaacg accttgaagg
     2521 aagcgttagc tgaacgggat aacataccac caagcctgga tgagcttggg gcagaattga
     2581 aggcgtttgt tgaaagctcg tttgatgaac tcaatgaggt aatgcagctt aattcattga
     2641 ttaacggtgc gctggactca atgatgaagc aattccaggt ggaagaggag cggaagttgc
     2701 aacaggaacg actaattgaa gccgaaaagc aaagagacct ggagagggaa agagaaagag
     2761 tgcgccagcg tgcgtattcc tcaccagcac catcacgttc acgtgattat ggtatgagcc
     2821 gttaagtact ggaggttttt attttatgca attggatgaa cattcaagaa aaattgctgc
     2881 tcttgttgcg tataatgata aacaaataaa agaaatgaac aggctaatta ctctgcttaa
     2941 tgaaagagtg aatgacttaa atacctttac aagtactctt gaacgtgaag gtaaagttgt
     3001 ttctggaatt tcttattcct ttagggatag tgtgtctaat gttaaatttt atattaataa
     3061 aggaatggat gaggcatctt gtaaattaca gaaatcagtc tcagatacac taacgtcatt
     3121 actccgcgaa aaattatctt tcactctcaa tgatgtaaag aaagagtggg ttgatttcga
     3181 ggagcaaaca agtaaagaga taaaggaatc attaaagcag acgaccagag aggccgaagt
     3241 tgcgacaggt ataattagaa aaacaatgga gaagtataca gtaacaagga ttgtcgaaaa
     3301 ggtaattgtg ttgattgtat tatttttatt gctaatgatc gctcctgttt tctatgctga
     3361 atatctcaaa atgaatatta gttcgctaat ggaagaacag aaaaaacatg aatccatgtt
     3421 aaaagaatgg aaagaaaaag ctccattggc cgagttctcg aagtgtggag attatcgtaa
     3481 agcatgcgtg ttagttgatg acgaaagcgg atattatttt aatgaacaat caccaaataa
     3541 aaaatatgcc atactgaaat agtgttaagt aactcatgcc gtcttatgac taaggctgca
     3601 tgagtataaa aacggcgttt cacgtaaaaa taaaataaat acctgtacct aatataaaaa
     3661 tggaactaca tatgcttatg tatcttgaga atgatggcgt caatttatcc cttatcgtta
     3721 agctaatagc tatgtaaata agatctgtta ggaatgatat aaatataaat aaaacaccca
     3781 atataaaaaa gtttaatgtg atatttgtat tattatctat aaaggttggt attaacgcag
     3841 caaagaaaag atacgccttg gggtttaata atggtatcaa aaaaggtttt tgctcttttc
     3901 tttgtttgtt ttttttatta attaaaccca ttaatcctat gtatattaaa tagcctccac
     3961 cgaatatttt tataatgtgg aaatgttgtt ctgtgctgga tgttgaggat aacgcgaaaa
     4021 gagatattgc catgacgata gtctcgccaa ttcttatgta tatacttgtt tttattgatg
     4081 actttaatcc atgtaaaaag gcattgttta taaccaatgc aacgccaggc cctattgata
     4141 tagtttgcga taaggttacg atagcaaatg ataataatgt tgaatattcc atgtttactt
     4201 accatgatat atttcgtggg agcttttcat atacatattc cataggaatt gcagctttcg
     4261 gtaataaatt tataaatgtt gatatgtgtt ttagtgaatt gctggcggcg attgagtaaa
     4321 aatttccgct attccaatat attgatgcgt atttaaaatg ttgtttaatt gcggaaagca
     4381 tatcgtcaac actaacttca ttcaaattca ttgtgtttag tacgatttcc tttttggtgt
     4441 taatctcatc tttcgagaaa tacctttgtt tgaatgaata atctttttgg atttctcttt
     4501 tttgatattc atcatgttct gaactatgtt tttctgtaaa tatgaaaata ccatcattat
     4561 ttaatagcct ttttacgtgg tttatttgcc cagatctgtt aggtgagtac atttgaaatg
     4621 tggtgtcttc tattataata tcgaatcctt gattaaattg tttaagattt gaatttttta
     4681 gtctttcctc taacaggaaa aatggagtgc aaataaaata agcgttttca cttgagccat
     4741 acttaaaaaa tgcatgttca ttttcttttg ttggactggt tgttaatgtt tgtatgtttc
     4801 cttttgataa ttctgaaatg gttcttgcca ttgcgccctc agccatacca agacaccatg
     4861 tgtttaattt tttgtttagc gtgattgcgt attttattac tgttgttcct aatctgcact
     4921 cttcttcctt gctgtatggt atactgcaaa aataatgatt aacgaaaggg ccgctgtttt
     4981 ttgtgaggtg tttggcaaac ttaatgtaat cataagaatg aaaaagccga tcatctattt
     5041 ctactagtct aaaaggtttg tgtttttccc ctgatgcagt tgatgagaaa aattctgcca
     5101 tgccgtttat tctcggagcg gaaatacatt catttataaa attattcatt ttcctatcct
     5161 ctggtttgat ttttggaatt tgttgttatg actcttcaat gctaattctc ttgtaagctt
     5221 ttttacacgc cataaacacg attatgatgt tttatattgt cgatatttcg ttgtccagac
     5281 tggatatgtt tggaaatttg ttttcatgcg ggttagttca gctggctttc tggatgagaa
     5341 agtttgctgt tttgaccagt tcgcgcttct gaaggggtga aaccgatcat taatcgcttt
     5401 tgtgcatagt tatgcaggcc cctaaaaacg attctgacgc gttttttgtg ttttccctag
     5461 gtttatcttg tctttttgag ttttttgcgt cagaaagcga ttgagagcgt tttagagggt
     5521 acgtacaagg ggagttatgg taaatggatc gggttttcgt gatggatcga cagggtttgt
     5581 cgttgggtgt agtgtaagca gctgaaaaac aaacgcccct gaaattcgtg ccgatccgcc
     5641 aagactgtca cgaaaattca aggggcgtgt gggttgtaac acacagagga aatcctaaca
     5701 c
//
