LOCUS       NZ_CP074188            13936 bp    DNA     circular CON 13-MAY-2024
DEFINITION  Raoultella planticola strain JBIWA001 plasmid pJBIWA001_6, complete
            sequence.
ACCESSION   NZ_CP074188
VERSION     NZ_CP074188.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18916998
            Assembly: GCF_019968885.1
KEYWORDS    RefSeq.
SOURCE      Raoultella planticola
  ORGANISM  Raoultella planticola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 13936)
  AUTHORS   Gomi,R., Matsumura,Y., Tanaka,M., Ihara,M., Sugie,Y., Matsuda,T. and
            Yamamoto,M.
  TITLE     Emergence of rare carbapenemases (FRI, GES-5, IMI, SFC and SFH-1) in
            Enterobacterales isolated from surface waters in Japan
  JOURNAL   J Antimicrob Chemother 77 (5), 1237-1246 (2022)
   PUBMED   35137119
REFERENCE   2  (bases 1 to 13936)
  AUTHORS   Gomi,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2021) Department of Environmental Engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 135x
            Sequencing Technology             :: DNBSEQ; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019968885.1-RS_2024_05_13
            Annotation Date                   :: 05/13/2024 04:14:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,989
            CDSs (total)                      :: 5,866
            Genes (coding)                    :: 5,727
            CDSs (with protein)               :: 5,727
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 46 of 139
            Pseudo Genes (incomplete)         :: 109 of 139
            Pseudo Genes (internal stop)      :: 34 of 139
            Pseudo Genes (multiple problems)  :: 44 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074188.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13936
                     /organism="Raoultella planticola"
                     /mol_type="genomic DNA"
                     /strain="JBIWA001"
                     /isolation_source="lake water"
                     /db_xref="taxon:575"
                     /plasmid="pJBIWA001_6"
                     /geo_loc_name="Japan: Shiga"
                     /lat_lon="35 N 136 E"
                     /collection_date="2020-02-05"
                     /collected_by="Kyoto University"
     gene            join(13926..13936,1..247)
                     /locus_tag="KGP19_RS29755"
                     /old_locus_tag="KGP19_29615"
     CDS             join(13926..13936,1..247)
                     /locus_tag="KGP19_RS29755"
                     /old_locus_tag="KGP19_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            355..510
                     /locus_tag="KGP19_RS29760"
                     /old_locus_tag="KGP19_29620"
     CDS             355..510
                     /locus_tag="KGP19_RS29760"
                     /old_locus_tag="KGP19_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(668..829)
                     /locus_tag="KGP19_RS30055"
     CDS             complement(668..829)
                     /locus_tag="KGP19_RS30055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            1234..1425
                     /locus_tag="KGP19_RS29765"
                     /old_locus_tag="KGP19_29625"
     CDS             1234..1425
                     /locus_tag="KGP19_RS29765"
                     /old_locus_tag="KGP19_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1425..1847
                     /locus_tag="KGP19_RS29770"
                     /old_locus_tag="KGP19_29630"
     CDS             1425..1847
                     /locus_tag="KGP19_RS29770"
                     /old_locus_tag="KGP19_29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2758..>3552)
                     /locus_tag="KGP19_RS29775"
                     /old_locus_tag="KGP19_29635"
                     /pseudo=""
     CDS             complement(2758..>3552)
                     /locus_tag="KGP19_RS29775"
                     /old_locus_tag="KGP19_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /name="methyl-accepting chemotaxis protein"
     gene            3647..5002
                     /locus_tag="KGP19_RS29780"
                     /old_locus_tag="KGP19_29640"
     CDS             3647..5002
                     /locus_tag="KGP19_RS29780"
                     /old_locus_tag="KGP19_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997539.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISPme1 family transposase"
                     /protein_id="WP_012112698.1"
                     /translation="MDHLEGAGLARGDRVDFDRRVRLEFRGAQISSDGGLLVMRELDDV
                     LGLSNLASEALRDSRTGKNTLHRLDGLFRQSVFGRLAGYEDVNDADRLALDPVMRQVVG
                     GRAVEAQAASASQMGRFETETLALAANRAALADLNGQWIDRFHDRNGLKYIVLDMDSSV
                     SPTHGDQEGAAWNGHFDCTCYHPIFLFNQFGMLERCALRNGNVHSADGWRDVLDPVIAR
                     YAGRDLGGRFFRADAAYAIPAIYMRLEEARFFYAIRLPANAVLREKIAHRLTRPVGRPS
                     LTKVKRFFEDFEYQAASWDKPRRVIAKIEWHPGELFPKVGFIVTNLPMEPDWVVRFYNQ
                     RGTAEQHIKEGKYAFRWTRLSCRKFRHNEVRLQLHALAYNLATFLRCIELPEAMADWSL
                     TSLQLKLIKIGARVVRHARAITFQLAEVAVTGPMVRAVLAAIRRLRTPPSCA"
                     /name="IS1380-like element ISPme1 family transposase"
     gene            5244..6074
                     /locus_tag="KGP19_RS29785"
                     /old_locus_tag="KGP19_29645"
     CDS             5244..6074
                     /locus_tag="KGP19_RS29785"
                     /old_locus_tag="KGP19_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542600.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_096143331.1"
                     /translation="MGYVTTADGVQIYYKDWGPKDAPVIFFHHGWPLSADDWDAQMMFF
                     LGEGFRVIATDRRGHGRSEQVWDGHDMDHYADDTAAVVKNLGVQGAVHVGHSTGGGVVA
                     RYIARHPEDKVAKGVLISSVPPLMVKTADNPEGIDKSVFDDFQKNTKENRAQFFYDVPA
                     GPFYGFNRPGAKASEPVIQNWWRQGMMGGAKAHYDSIVAFSQTDFRDDLKSITTPMMVM
                     HGLDDQVVPFEISGQKSAELLQNCVLKTYPGFPHGMPTTEAETINKDLLAFIRS"
                     /name="alpha/beta hydrolase"
     gene            6178..7635
                     /locus_tag="KGP19_RS29790"
                     /old_locus_tag="KGP19_29650"
     CDS             6178..7635
                     /locus_tag="KGP19_RS29790"
                     /old_locus_tag="KGP19_29650"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434365.1"
                     /GO_function="GO:0004096 - catalase activity [Evidence
                     IEA]; GO:0020037 - heme binding [Evidence IEA]; GO:0046872
                     - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="WP_096143330.1"
                     /translation="MSDKPKLTTAFGAPVPDNQNTMTAGPRGPALLQDVWFLEKLAHFD
                     REVIPERRMHAKGSAAHGTFTVTHDITPYTKAKLFSDIGKKTDMFVRFSSVAGERGAAD
                     AERDIRGFAMKFYTEEGNWDLVGNNTPVFFLRDPLKFPDLNHAVKRDPRTNMRSADNNW
                     DFWTNLPEALHQVTIVMSDRGIPASFRHMHGFGSHTYSFLNANNERFWVKFHFRTQQGI
                     KNLTDAEAAETIAHDRESNQRDLYENIEAGNFPKWTLFVQIMPETDATTYHLNPFDLTK
                     VWPKGDYPLIEVGEMELNRNPDNYFAEVEQAAFAPTSIVPGISFSPDKMLQGRLFSYGD
                     AQRYRLGVNHAQIPVNAPRCPVNAYHRDGAMRVDGNYGSRLHYEPNMFGEWQEQPDFSE
                     PPLAIDGAADHWNQRVDEDYYSQPGNLFRLMTPDQQQALFGNTARAIGPARIEIKHRHI
                     RNCLRADPAYGKGVADALGIDLADVGA"
                     /name="catalase"
     gene            complement(<8289..9077)
                     /locus_tag="KGP19_RS29795"
                     /old_locus_tag="KGP19_29655"
                     /pseudo=""
     CDS             complement(<8289..9077)
                     /locus_tag="KGP19_RS29795"
                     /old_locus_tag="KGP19_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA];
                     GO:0007165 - signal transduction [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            9387..9947
                     /locus_tag="KGP19_RS29800"
                     /old_locus_tag="KGP19_29660"
     CDS             9387..9947
                     /locus_tag="KGP19_RS29800"
                     /old_locus_tag="KGP19_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            9951..12917
                     /locus_tag="KGP19_RS29805"
                     /old_locus_tag="KGP19_29665"
     CDS             9951..12917
                     /locus_tag="KGP19_RS29805"
                     /old_locus_tag="KGP19_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="WP_223526270.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRGD
                     ANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRREHA
                     QELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALSVID
                     RACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKPNSRH
                     MLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYATLAAV
                     VLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIGQALLEA
                     KESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRYTPALLEV
                     LELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKFYE
                     ICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYLE
                     ERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLPRI
                     KITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPGLTY
                     AKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRGESSG
                     HVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIEEHYTD
                     TAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNIKHVRAH
                     WDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVEL
                     RRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYL
                     ERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFRPLRMPGKP"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            complement(12982..>13323)
                     /locus_tag="KGP19_RS29810"
                     /old_locus_tag="KGP19_29670"
                     /pseudo=""
     CDS             complement(12982..>13323)
                     /locus_tag="KGP19_RS29810"
                     /old_locus_tag="KGP19_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <13322..13924
                     /locus_tag="KGP19_RS29815"
                     /old_locus_tag="KGP19_29675"
                     /pseudo=""
     CDS             <13322..13924
                     /locus_tag="KGP19_RS29815"
                     /old_locus_tag="KGP19_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B-related nuclease bacteriocin"
                     /name="klebicin B-related nuclease bacteriocin"
     Typing          complement(717..1186)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.834"
                     /coverage="92.0"
                     /evalue="2.73e-170"
ORIGIN
        1 taaaatttca gattttacgg aagaagaatt tactgctttt gttagtaaaa taatcgctgc
       61 tgattttcca actgacaaag agaacgatga tgctatttat tcgttcgccc aactaacaga
      121 gcatcctgct ggttggaacc tcatcttcaa gcctaaggta ggcgaagatt cttcggcgaa
      181 aggaattgtt aaaaccgtaa aggaatggcg agctgctaac ggtaagccag gttttaaaaa
      241 agcgtgattg aatttgcggg ttcaagttat gagcccgctc atttgtaact ctggatttga
      301 agaataatta ctagatatcc atttgcctag aataataaaa ataaaaggtg atatatgaaa
      361 aaattacttt tcctaggggt tgttaccgtt tctgtggttc tggcagcatg tcaggtcaat
      421 aatgttcgag acaccggagg tggggctgtt tcaccttcat cgacggtgac cggagtcacg
      481 cttgatagcg gtacgaacgg caatccgtaa aggatcttct tgagatccca tttggatcgt
      541 cgtaatctct tgctctgtaa acgaaaaaac cgccttgcag ggcggttttt ccgaaggttc
      601 aaacacttgc aggtgattga accaaggtaa ctggcttaga ggagcgcagt caccaaaaat
      661 tgttctttca gtttagcctt aacaggcgca tgacttcaag accaactcct ctaaatccgt
      721 taccagtggc tgctgccagt ggcgctttgt cgtgtctttc cgggttggac tcaagatgat
      781 agttaccgga taagacgcag cagtcggact gaacgggggg ttcgtgcata cagtccagct
      841 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
      901 aacagcggaa tgacaccggt aaaccgaatg gcaggaacag gagagcgcac gagggagcca
      961 tcagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     1021 cgtcaaattc tgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ccgctgtgct
     1081 gccttctccc tctccgagct gtctgctcta tgtcgttatg tgcgcgttgc gaccgctcgc
     1141 cgcagccgaa cggcaggagc gtagcgactg agtgagcgag gaagcggaaa agagatctgt
     1201 ctgacattga gcactgacgc tctgaggccc tttatgacga aacaacagaa aacagcgctg
     1261 aacatggcta aattcatcca gaatcagtct ctgctcctgc tggagaagct taacgagctt
     1321 gatctcgatg ttgaggccga cctttgtgag aaactccacg atgatgcgga gcatcttttc
     1381 cgtacactgt cctcgagact ggatgaatta caggatggca attgatggcc agagtgtcta
     1441 ttagtgaagc ggcacggctt gttaaggtta gccgtcccac aatttacaag atgattaaca
     1501 gtgggaagtt aagttacacc tctgttgtaa agcacgggaa ggctattaaa gttattgata
     1561 cttcagagct tattagagtt tttggttctc ttgatggtgt catcgatact gtaaaatatg
     1621 atgtaaaaag tgacgcagaa tctacaagta ttaacagcgt tggtttacat gatttacaac
     1681 atagaatcgc tctacttgaa gctgaaaacg atggtcttaa aggggctgtt aaggctcgag
     1741 atgagcatat tgattccctt cgtcaggcta tgcagcttct ggagcacaag cgcgagcctt
     1801 cttcaccacc ccattccccg tggtggaaat tctggaaaaa atcctgatcc tgccatgctt
     1861 gagttttcct ctccggccat cccagggagt tcctgaacct tcccaaaaat cgccagatct
     1921 ctgaacgctc ctcacgacta aaaataccca cacactacgc aaaattaaaa ttttgcagat
     1981 tccttattta aaacaattgg ttaatgaaaa attaatgtgc agtgatgtgt tacaaagtgc
     2041 agatccaagt cgtggacgac tctatggcga atcgtttacg aattgcccac gattcatatg
     2101 tgaatcacgc tttattatca tgttgttatg atgaatcgac tcttgattca attcatcaat
     2161 ttgggcgttt tttgttcatg gtgactcttg attcaattca cgattcgaat cgccggtgaa
     2221 tcgtgtggtg agtcgtccgg cgacttgcca taacgtgatt gccggccgtc gacgaacccg
     2281 attcctgaac gtgctgaaaa ccggttttcc ggctgcgccg gcaacgcctc acggccacgc
     2341 aataaattgc gtccccgttc accgttttcg gatcagaaga acgccggaaa caggacttac
     2401 cctggctgaa tggcgtgaat ttccgcgcta tgcacttgcg cgcatactca tgcatgccgt
     2461 aaaaacagag cctgcgcgtt tctggcgggt tttcgggtgg tttgttgcct gttttaccgg
     2521 tttcccgtca gaaacgtcct gaggccgttt tagcggtgcg tacaattagg gggaaccgca
     2581 gaattcggaa aaaatcgtac gctaagctaa cggtgttctc gtgacagctc tttgactagg
     2641 ctttctaagg ccattctgat agccctgact tcctgaaaag ccatggctaa aatttgtgcg
     2701 gctaaaaggg ataaccgatg gtaaagtaag ttatccctgt cgagatactg aaaagcgtta
     2761 tcctcgtttt tcccaaaact gttttgccag ttcgctcaga gcgctagtta actgagcgac
     2821 agatttcgca ctttgcaaat tattctgccc ggtctgtaca ttggtatcag cagcatcgcg
     2881 tatattgata atactgcggt ttatgtcttc acttactgct ccctgctgct cgaccgcagt
     2941 cgctatttgc gcgttcatgt cggtaatttc gttaacgcgt tggccaattc catcaagagc
     3001 tgtagctgct tcctctgcgt gagctacact cgtgtgcgct tgccgactac tttgctccat
     3061 gactgtaaca gcggattgcg ctcgctcttg tagagcgctg atcatgcttt gaatatccgt
     3121 tgtcgattgc tgtgtgcgag cagcaagact gcgaacctca tcggcgacaa cagcaaaacc
     3181 acgcccctgc tcaccagcac gcgcggcctc aattgctgcg ttgagtgcca acaaattcgt
     3241 ttgctcggcg atccctcgta taacgtcaag aacttttgat atctcgttac tttgaccttc
     3301 aagctcatga ataacctgag tggcttgcct aatttcacct tcaagggcag tgattgactg
     3361 gcttgtgtgg gctaccagac gctggccaga tgccgtctca gtgtctgctc ttccggccgc
     3421 atctgcagca tgctgtgcat tgctcgcaac ctcttgaatg cttgccacca tttggtttac
     3481 tgccgttgct atttgatctg tctctgcctg ctgctcaact gtaagtacat tgcttgactc
     3541 aatatccttt agcccacatt ctccaagtaa gtcgctgatt tcgctgtcgt aatcgtgata
     3601 tttcgcatat aaatcatggc gttgacggct gcgaggggcg cgtttcatgg atcacctgga
     3661 gggtgcgggc ttggcgcggg gagatcgggt tgatttcgac cgccgtgtgc gtctggagtt
     3721 ccgtggtgcg cagatcagtt cagacggtgg cctgctggtg atgcgcgagc ttgatgacgt
     3781 gctcggcctg tccaatctgg cgtcggaggc gctgcgagac agccgcaccg ggaagaacac
     3841 gctccatcgg cttgacggat tgttccggca atcggtgttc ggacgactgg ccggatacga
     3901 ggatgtgaac gatgccgacc gcttggccct cgatcccgtg atgcgtcagg tcgttggcgg
     3961 cagggccgtc gaggcgcaag ctgcttcggc atcgcagatg ggacggttcg agaccgagac
     4021 gctggctctg gccgcgaacc gggcggcgct ggccgatctg aacggccaat ggatcgaccg
     4081 gtttcatgac cgcaacgggt tgaaatacat cgtgctggac atggacagct cggtcagccc
     4141 cacccacggc gatcaggaag gtgctgcctg gaacgggcat ttcgactgca cctgctatca
     4201 ccccatcttc ttgttcaacc agtttggcat gctggagcgc tgcgccctgc gtaacggcaa
     4261 tgtccacagc gccgatggct ggcgggatgt ccttgatccc gtcattgccc gatatgctgg
     4321 ccgcgacctt ggtggacgct tcttccgggc cgacgctgcc tacgcgatcc ccgcgatcta
     4381 tatgcggctg gaagaagcca ggttcttcta cgccatccgt ctgcccgcca acgccgtctt
     4441 gcgcgagaag atcgcgcatc ggctgacacg gcccgtggga cggccttcgc tgaccaaggt
     4501 caaacggttc ttcgaggact tcgagtatca ggcggcgtcc tgggacaagc cgcgccgcgt
     4561 catcgccaag atcgaatggc atccgggcga gctgttcccc aaagtcggct tcatcgtcac
     4621 caacctgccg atggagccag actgggtggt gaggttctac aaccagcgcg gcaccgcaga
     4681 gcagcacatc aaggaaggca aatatgcctt tcgctggacg cggctgtcat gccggaagtt
     4741 ccggcacaac gaggtgcggc tgcaactgca cgcgctggcc tacaacctgg caaccttcct
     4801 gcgctgcatc gaactgcccg aggccatggc ggactggtcg ttgaccagcc tgcaactcaa
     4861 gctgatcaag atcggcgccc gcgtcgtccg ccacgcccgc gccattacct tccagttggc
     4921 cgaggtcgcc gtcaccggcc cgatggtgcg ggccgtcctt gccgccatcc gccgtcttcg
     4981 aacgcctccg tcatgcgcat gaccacgatc catgcccaag ctgaacgaaa gcggcaggac
     5041 aggtccgtct gccgcgcgga aaagcggctc tgccgggcca gaatgctgcg ggttcgaggc
     5101 ttgatccacc cgacttcggc cgtttgcgcg acgacagaca ccgctcgggg cgaaaaacgc
     5161 ttgcccagcg agcaaaatca ggcgatcttg aagtcaagcg gcaggccact tggggaatgt
     5221 cgggatatat cggagcaata cgaatgggtt acgttaccac cgctgatggc gtccagatct
     5281 actataagga ctggggtccg aaagacgctc cggtcatctt cttccatcac ggctggccgc
     5341 tgagcgcgga tgactgggat gcgcagatga tgtttttcct gggcgagggc ttccgcgtca
     5401 tcgccaccga ccgccgcggg catggccgct cggaacaggt ctgggatggt cacgacatgg
     5461 atcactatgc cgatgacacc gccgccgtgg tgaaaaatct gggcgtgcag ggcgccgttc
     5521 atgtcggcca ttccacgggg ggcggcgtgg ttgcgcgcta tatcgcgcgg cacccggaag
     5581 acaaggtggc caagggcgtg ctgatcagct cggtcccgcc gctgatggtc aagaccgccg
     5641 acaacccgga ggggatcgac aaatcggtct ttgacgactt ccagaagaac accaaggaaa
     5701 accgcgcgca gttcttctat gacgtgccgg cgggcccgtt ctacggcttt aaccgtcccg
     5761 gcgcgaaggc gtctgaaccg gtgatccaga actggtggcg tcagggcatg atgggcggcg
     5821 ccaaggcgca ttacgacagc atcgttgcct tttcgcagac cgatttccgc gatgatctga
     5881 aaagcatcac cacgccgatg atggtcatgc acggactgga cgatcaggtc gtcccgttcg
     5941 agatctcggg ccagaaatcg gccgagctgc tgcagaactg cgtgctgaag acctatccgg
     6001 gcttccccca cggcatgccg accaccgaag ccgaaaccat caacaaggat ctgctggcgt
     6061 tcatccgcag ctgatgatct tctggtggcc agcggcgcaa tccgctggcc atttttctga
     6121 atatatttag cgacggccgg ggccttgcgc gcgcctcgtg cccgccagga ggttatgatg
     6181 tccgacaagc ccaagctgac aaccgcattc ggcgcccccg ttcccgacaa ccagaacacc
     6241 atgacagccg gtccgcgtgg gccggctttg ctgcaggatg tgtggtttct ggaaaaactg
     6301 gcccatttcg accgcgaggt gatccccgaa cggcggatgc atgccaaggg atcggccgcg
     6361 catggcacct tcaccgtcac ccatgacatc acgccttaca ccaaggccaa gctgttttcc
     6421 gacatcggca agaaaaccga tatgttcgtg cgcttttcca gcgtggcggg ggaacgcggc
     6481 gcggcggatg ccgaacgcga cattcgcggt ttcgcgatga agttctatac cgaagaaggc
     6541 aattgggatc tggtgggcaa caacacaccc gtctttttcc tgcgcgatcc gctgaaattc
     6601 cctgacctga atcacgccgt caaacgcgac ccgcgcacaa atatgcgcag cgccgacaac
     6661 aactgggatt tctggaccaa cctgcccgag gccctgcatc aggtcaccat cgtcatgtcc
     6721 gatcgcggca tcccggccag ctttcgccac atgcacggct ttggcagcca cacctatagc
     6781 ttcctgaacg ccaataacga acgtttctgg gtgaaattcc acttccgcac ccagcagggc
     6841 atcaagaacc tgaccgatgc cgaggcggcc gagacgattg cccatgaccg cgaaagcaat
     6901 cagcgcgatc tgtatgagaa tatcgaggcc gggaatttcc ccaaatggac cctgttcgtc
     6961 cagatcatgc cagaaaccga cgcaaccacc tatcacctga acccgttcga tctgacgaag
     7021 gtctggccca agggcgacta tcccctgatc gaggtcggcg agatggagct gaaccgcaac
     7081 cctgacaact atttcgccga ggtcgaacag gccgccttcg cccccaccag catcgtgccg
     7141 ggcatcagct tttcgcccga caagatgttg caggggcggc tgttttctta tggcgatgcg
     7201 cagcgttacc ggctgggcgt caaccatgcg cagatcccgg tcaatgcgcc gcgctgcccg
     7261 gtcaacgcct atcaccgcga cggggcgatg cgggtcgatg gcaactatgg ctcgcgtctg
     7321 cattacgaac ccaatatgtt cggcgagtgg caggaacagc ccgatttttc cgaaccgccc
     7381 ttggcgattg acggcgcggc ggatcactgg aaccagcggg tggatgagga ttactactcg
     7441 cagcccggca acctgttccg gctgatgacg cccgaccagc agcaggcgtt gtttggaaac
     7501 accgcgcgcg ccattggccc ggcccggatc gaaatcaagc accgccatat ccgcaactgc
     7561 ctgcgcgccg atccggctta tggcaagggc gtagccgatg cgcttggcat tgatctggcg
     7621 gatgtcggcg cctaggccat cgcggcgcgc atgacggcgc tgtcccccaa ggctgaacgg
     7681 cgggggcggc gacacgtctg caagatgctg ggacatcagc cgctgtggtg ccatcggccc
     7741 tgcacggctg gcatcggaaa ggcgtttatg cgtcagtcct ctgaccggcc ggggcgcggc
     7801 gtctgctgac ttgcggcgcg cgttgcaaaa gccgtgcggt ctggtccgag cgagcctttg
     7861 cgcgaaacga aaaagccggg cccatgcggg gcctggcttc ttatctgatg ggatggctta
     7921 ttccgcggcg cgtttgggca ggacccagga atatgtgtcc tagctgaaat cgcagggcaa
     7981 atttttgact tttcccgatc tgtcggccaa ccagtttact cctcggtcgt gttcgcagcc
     8041 tcaaccctgg ttcttgcgct gcctctctac ctggtttgga aggcaatctt cacgaatgac
     8101 ccatactcaa aataacatcc ttgaccctga cctcctgcga cccatagctg gatccacttg
     8161 tcttctagcg acgacaagca aactgccgcg ccttcgggcg cggctctttt cgttcagctt
     8221 gtccaccccg gtcctcggac cggggcttgc agggcatgag cgagacgtca aggatcgagc
     8281 gaatgccctt ttagtaggcc tcgggtgtgt tcgctaagcc gatttgatgc atcacctatg
     8341 cgacctacta tggcgcctgt ttcagcttgc atcattcgta aagcaaactc tatttggcca
     8401 aactcatcgg tgcgcccagt gtagagggat tgacttaatg ggttattgga aatattcctg
     8461 gctctttcaa ccagtcttcc aagaggagag agaatagcca aaacactaac agagcttaag
     8521 cttcctgaca ttaaagtggc taacaataag ctgcttattg atgtatcagt aagcatgccg
     8581 gcagccattg cgcttgatat aatactaccc catatgagca agagtatttt cacggaaaag
     8641 ctagcagcca atttcggcct cgcggccttc ccgcttctca attgagcata taatttttcc
     8701 gcagccaaaa cctgctcagg ttcaggcttg gtccttacag actggtattc aacaatcgaa
     8761 ccattcttag ctattggcgt tacataagca cttacccaat agtggtcgcc atttttacag
     8821 cgatttttta ctagccccat ccatgagcgg ccagatttta atgtactcca catatgctca
     8881 aatgcagcag gcggcatatc tgggtgtctt acgatgttgt gaggctggcc taatagttct
     8941 tcctcagtga aaccactgat tttaatgaag tcaggattaa cgtacgtgat atggctttga
     9001 ggggaggtag tcgaaagaat attggcatct tttgggagtt ctaagtttcg acccgtcact
     9061 ggtaaattct ggcgcataag aacctcaagg gttggctgtt ttattttatt gttttcggca
     9121 ttaagcccaa tttcttgagc gttacgataa agctagcatg gaaacgatag gtgcaagcaa
     9181 gttaagggtt gcatcgcgca tgtcaatcta ggctataccc taacttgatg tcaggcaggg
     9241 ccgcgccgct tcgtcagaat agagtctgct ttcccatttt ttgacacatg cccgcgaagg
     9301 ttatagattt cagcctgaca gaaatgggct ttgaggcaca acggaacaga aagtgcactt
     9361 aagccgcctt caaccaagga gacatcgtgc aggggcaccg catcggctac gtccgggtca
     9421 gcagctttga ccagaacccg gaacgccagc tggaacaaac ccaggtgagc aaggtgttca
     9481 ccgacaaggc atcgggcaag gacacccagc gcccccagct cgaagcgctg ctgagcttcg
     9541 tccgcgaagg cgatacagtg gtggtgcaca gcatggatcg gctggcccgc aacctcgatg
     9601 acctgcgtcg cttggtacag aagctgactc agcgcggcgt gcgcatcgag ttcctgaagg
     9661 agggcctggt gttcactggc gaggactcgc cgatggccaa cctgatgctg tcggtgatgg
     9721 gggccttcgc tgagttcgag cgcgccctga tccgcgagcg gcagcgtgag ggcatcgcct
     9781 tggccaagca gcgtggcgcg taccggggcc gcaagaaagc cctgtccgat gagcaggctg
     9841 ctaccctgcg gcagcgagcg acggccggcg agcccaaggc gcagcttgcc cgcgagttca
     9901 acatcagccg ggaaaccctc taccagtacc tccgcacgga cgactgacac atgccgcgtc
     9961 gcttgatcct ctcggccacg gagcgggaca ccctgcttgc gctgccggaa agccaggatg
    10021 acctgatccg ctactacacc ttcaacgact ccgacctgtc gctgatccgc cagcgacgcg
    10081 gcgacgccaa ccgcctcggc ttcgccgtgc agctctgcct gctgcgctac cccggttacg
    10141 cgctgggaac cgacagcgag ctgcccgagc cggtcatcct gtgggtggcg aagcaagtcc
    10201 aggccgagcc ggcgagctgg gcaaagtacg gcgagcgcga cgtgacccgt cgcgagcatg
    10261 cccaggaact gcgcacctac ctgcaactgg ccccgttcgg cctgtccgac ttccgcgccc
    10321 tggtgcgcga gctaaccgag ctggcccagc agaccgacaa aggcttgctg ctggccggtc
    10381 aggccctgga gagcctacgg cagaaacgac gcatcctgcc ggcgctgagc gtgattgacc
    10441 gggcctgctc ggaagccatt gcgcgagcca atcggcgggt ctaccgcgcc ctggtcgaac
    10501 cactcacgga ctcgcatcgg gccaagctgg acgagctgtt gaagctcaag gccggcagca
    10561 gcatcacctg gttgacctgg ctgcgccagg caccgctgaa acccaactct cggcacatgc
    10621 tggaacacat cgagcggctg aagacatttc agttggtgga cttgcccgaa ggcctgggcc
    10681 ggcacatcca ccagaaccgc ctgctcaagc tggcccgcga gggtgggcag atgacgccca
    10741 aagacctcgg taagttcgag ccgcagcgcc gctacgcgac cctggccgcc gtggtgctgg
    10801 agagcaccgc gaccgtgatc gatgagctgg tcgatctgca tgaccgcatc ctggtcaagc
    10861 tgttcagcgg cgcgaagcac aagcatcagc agcagttcca gaagcagggc aaggcgatca
    10921 acgacaaggt gcgcctgtac tccaggatcg gccaggcgct gctggaagcg aaggaaagcg
    10981 gcagcgaccc ctatgccgcc atcgaggcgg tgattccctg ggacgagttc accgagagcg
    11041 tcagcgaggc cgagctgctg gcccggccgg aaggcttcga ccacctgcac ctggtcggcg
    11101 agaacttcgc caccctgcgc cgttacacgc cggccttgct ggaggtgctg gaactgcgcg
    11161 ccgcgccggc cgcgcaaggc gtgctggcag ccgtgcagac cctgcgtgag atgaacgccg
    11221 acaacctgcg caaggtgccg gccgatgcac ccacggcctt catcaagccg cgctggaagc
    11281 cgctggtgat caccccggaa ggcctcgacc ggaaattcta cgaaatctgc gccctgtccg
    11341 agctgaagaa cgccctgcgc tccggcgaca tctgggtcaa gggctcgcgg cagttccgcg
    11401 acttcgacga ctacctgctg ccggccgaga agttcgccgc actcaagcgc gagcaggccc
    11461 tgcccctggc gatcaacccg aacagcgacc agtacctgga agagcgtttg cagctgctgg
    11521 acgagcagtt ggccaccgtc acccgcctgg ccaaggacaa cgagctgccc gatgccatcc
    11581 tcaccgagtc agggctgaaa atcaccccgc tggatgcggc ggtgccggat cgggcgcagg
    11641 cgctgatcga ccaaaccagc cagttactgc cgcgcatcaa gatcaccgaa ctgctgatgg
    11701 acgtggacga ctggacgggc ttcagccgcc acttcaccca cttgaaggac ggggccgagg
    11761 ccaaagacag gacgttgctg ctgtccgcaa tcctcggtga tgcgatcaac ctcgggctga
    11821 ccaagatggc cgagtcgagc cccggcctga cctacgccaa gctgtcctgg ctgcaagcct
    11881 ggcacatccg cgacgaaacc tattcggcgg ccttggccga gctggtcaac caccagtatc
    11941 gccacgcctt tgccgcccac tggggcgacg gcacgacctc atcctccgat ggccagcgct
    12001 tccgcgcggg tggccggggc gagagctccg ggcacgtcaa cccgaagtac ggtagcgagc
    12061 cgggacggct gttctatacc catatctccg accagtacgc gccgttcagc acccgcgtgg
    12121 tgaatgtcgg cgtccgcgat tccacctatg tgctcgacgg cctgctgtac cacgagtccg
    12181 acctgcggat cgaggagcac tacaccgaca cggccggctt caccgatcac gtctttgccc
    12241 tgatgcacct gctaggcttc cgcttcgcgc cgcgcatccg cgacctcggc gaaaccaagc
    12301 tgtacgtgcc gcagggcgtg caagcctacc cgacgttgcg cccgctgatc ggcggcaccc
    12361 tgaacatcaa gcacgtgcgt gcccactggg acgacatcct gcgcctggcc agctcgatca
    12421 agcagggcac cgtcaccgcc tcgctgatgc tgcgcaagct cggcagctac ccgcgccaga
    12481 acggactggc cgtggccctg cgcgagctgg gccggatcga gcgcacgctg ttcatcctgg
    12541 actggctgca aagtgttgaa ctgcgccgcc gcgtgcatgc cggcctgaac aaaggtgagg
    12601 cgcgcaactc gctggccagg gcggtgttct tcaaccgcct tggggaaatc agggatcgga
    12661 gcttcgagca gcagcgctac cgggccagcg gcctcaacct ggtgacggcg gctatcgtgc
    12721 tgtggaacac ggtgtacctg gaacgcgcca cccaggggtt ggtcgaggcc ggcaagccgg
    12781 tggacggcga gctgctgcaa ttcctgtcgc cgctgggctg ggagcacatc aacctaaccg
    12841 gcgattacgt ctggcggcag agccgcagac tggaagacgg gaagtttcgg cccttacgga
    12901 tgcccggaaa accttagcgt acgatttttt ccgaattctg cgggctcccc aattaaggga
    12961 ttatggtaaa tggtgacttg gttaatcata aacatcctgg gggatggcat acaccagttg
    13021 gatatccggt gcatcgttga gccatcgacc tgattttttc attaagtagc caccaatttc
    13081 ttcggtagtc agccgggcat gaaacaatgc ttcatcattg aaccagcagt cagggtgata
    13141 gatgagatcc agtgggtttg ccttgaaatt aatctccagt aactggagtg catagctgtg
    13201 gtcagatgca ctaccttctg ctttgcgtat gaagttgata acgtcgttca gttctttcca
    13261 tgtaaagtca tcgaggtatt tttcctgatt aagcgccatc ctggtgaaat ctagtgcact
    13321 tgacgcacct ggtgcaaacg gaccactgac gctaagcggg ccggtaccac taccggagcg
    13381 tattctgcat accggtgatc agatttcggc gccacaggct acagataaaa ccgtgacgcc
    13441 ggtagcggat gacctcgatt ttgacgatat tattctggtg tttcctccgg aatccggcct
    13501 aaaaccgctg tatgtgatgt accgtagccc tcgtaacatg cccggtacag tgagcggcaa
    13561 agggcagaac gttggcaata actggatggg gggggccagt accggggatg gtgctcctgt
    13621 tccttcccag attgcggata aattacgcgg gaagactttc ggtagttttg attctttccg
    13681 tcgggcgttc tggaaagcgg ttgctgacga ttctgcactg agcaagcaat ttagtgaagc
    13741 tgatattaat cagatgaaag ctggaagggc acctactgcg gactttttag aatcagtagg
    13801 gaaacgtgtt aaaattgaat tacaccacga aaaagaaata tctcaaggag gggctgttat
    13861 ggatgtcgat aacattaagg cgctaacacc taaaaatcat attgagacac ataaaggaaa
    13921 ataaaatggc tggtaa
//
