LOCUS       NZ_CP074125             6200 bp    DNA     circular CON 11-JUN-2024
DEFINITION  Escherichia coli strain EC16 plasmid pEC16-5, complete sequence.
ACCESSION   NZ_CP074125
VERSION     NZ_CP074125.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18877259
            Assembly: GCF_018316615.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Huang,J.
  TITLE     Whole genome sequence of EC16, an NDM-5-, CTX-M-55-, and
            FosA3-co-producing Escherichia coli ST167 clinical isolate from
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2021) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_018316615.1-RS_2024_06_11
            Annotation Date                   :: 06/11/2024 04:38:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,203
            CDSs (total)                      :: 5,080
            Genes (coding)                    :: 4,817
            CDSs (with protein)               :: 4,817
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 94 of 263
            Pseudo Genes (incomplete)         :: 176 of 263
            Pseudo Genes (internal stop)      :: 55 of 263
            Pseudo Genes (multiple problems)  :: 54 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074125.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC16"
                     /isolation_source="ascites"
                     /db_xref="taxon:562"
                     /plasmid="pEC16-5"
                     /geo_loc_name="China: Jinhua"
                     /collection_date="2019-08-25"
     gene            complement(587..736)
                     /locus_tag="KGQ53_RS25610"
     CDS             complement(587..736)
                     /locus_tag="KGQ53_RS25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1307..1465
                     /locus_tag="KGQ53_RS26305"
     CDS             1307..1465
                     /locus_tag="KGQ53_RS26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            1925..2740
                     /gene="sul2"
                     /locus_tag="KGQ53_RS25615"
     ARG             1926..2737
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2801..3604
                     /gene="aph(3'')-Ib"
                     /locus_tag="KGQ53_RS25620"
     ARG             2802..3601
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3604..4440
                     /locus_tag="KGQ53_RS25625"
     CDS             3604..4440
                     /locus_tag="KGQ53_RS25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(<4547..4951)
                     /locus_tag="KGQ53_RS25630"
                     /pseudo=""
     CDS             complement(<4547..4951)
                     /locus_tag="KGQ53_RS25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4995..5384)
                     /locus_tag="KGQ53_RS25635"
     CDS             complement(4995..5384)
                     /locus_tag="KGQ53_RS25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            5602..6042
                     /locus_tag="KGQ53_RS25640"
     CDS             5602..6042
                     /locus_tag="KGQ53_RS25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     ARG             3605..4437
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(772..945)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          869..1474
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgcacaagtg catagcgcgg aaattcacac cattcagccg gggtaagtcc tgtttccggc
       61 gttcttccca tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc gtgaagcgtt
      121 gccggcgcag ccggaaaacc cgctgtcagc acgttcagga agggggtgat gtacaaatgg
      181 cacagaggat gaaagtgacg tatgagctga aggaatgatg taaaagtgat gtataagtga
      241 tgtaaaagtg atgtatgaac tgaaggagcg atgtaattct gtagtcagga agaataaccc
      301 gttcatgcag gcatgaacga tacagtgagg aacgcttttt ttgtcacgat ctccccttaa
      361 cctgcgtaaa tcacccgcca tggttgatta tttttcaacc tcaaaatggt catacaccta
      421 acgcagccgc gattttcatc gcaactgcat gaattaaaag aaaaaaaagg aatatttcgt
      481 tttcacgagt aatacccggg tattaaaaat ccactgtaat tacatgaaaa taaagaaata
      541 aaaggtaata ccggagtatt accacgtacg cgggcaagcg aaaaagctat aacgcttccc
      601 ggccgacagg cgacccaaaa tccacagggg catgcaggtt ctcttcagta acctgcgcca
      661 ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc agtcgaatcc agttgttcat
      721 catattcaga tggcatgggg tgatatccag tagctgagag gttgttagcg ggtttcgggg
      781 cgcagccctg aaccagtcat gtagcgctag cggagtgtat actggcttac tatgttgcca
      841 cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga aaaaaggcag caccggcgcg
      901 tcagcagaat atgcgataca ggagatattc cgcttcctcg ctcactgact cgctacgctc
      961 ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg ggacggaaat ttcctggaag
     1021 ataccaggaa gacacttagc aaagaagaga taaggccacg gcgaagccgt ttttccatag
     1081 gctccgcccc cctgacaagc atcgcaaaat ctgacgctca aatcagtggt ggcgaaaccc
     1141 gacaggacta taaagatccc aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt
     1201 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcggtttc tcattccacg
     1261 cctgacactc agttccgtga aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     1321 cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa cttgagtcca acccggaaag
     1381 acacgacaaa acgccactgg cagcagccat tagtaacagg agtgcacgag aagatagatt
     1441 aagaggtctt gaagtagagg cctgactgcg gctacactga aagaacagtt ttggtaatgg
     1501 cggtctcgtg aaccctgtta ccgggttaag cagttcccca actgacttaa ccttcgaaca
     1561 aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga gattacgatt cattgaaaag
     1621 gatctcaaga agatcctttg tagattacta tcaattaatc ttccgaacat gacaccagtc
     1681 actgttgatt acggttaaac cgctccgtaa caggtttccc tcgtattggt gacttgcagc
     1741 accaaagaag caaaaaagat aaatatatct acttattgag agatgatata tttatttcca
     1801 aaatgctaca gatacatcat catgaaacgg atcaccgcaa acaggttact cgcctgggga
     1861 ttccctttcg acccgagcat ccgtatgaga ctcatgctcg attattatta ttatagaagc
     1921 ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg acagtttctc
     1981 cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta agctgatggc
     2041 cgagggggca gatgtgatcg acctcggtcc ggcatccagc aatcccgacg ccgcgcctgt
     2101 ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg gacgcgctca aggcagatgg
     2161 cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa gcctatgcct tgtcgcgtgg
     2221 tgtggcctat ctcaatgata ttcgcggttt tccagacgct gcgttctatc cgcaattggc
     2281 gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa gacgggcagg cagatcggcg
     2341 cgaggcaccc gctggcgaca tcatggatca cattgcggcg ttctttgacg cgcgcatcgc
     2401 ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc cttgatcccg gcatggggtt
     2461 ttttctgggg gctgctcccg aaacctcgct ctcggtgctg gcgcggttcg atgaattgcg
     2521 gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc aaatcctttc tgcgcgcgct
     2581 cacaggccgt ggtccggggg atgtcggggc cgcgacactc gctgcagagc ttgccgccgc
     2641 cgcaggtgga gctgacttca tccgcacaca cgagccgcgc cccttgcgcg acgggctggc
     2701 ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc gggatatttc
     2761 tctatattcg cgcttcatca gaaaactgaa ggaacctcca ttgaatcgaa ctaatatttt
     2821 ttttggtgaa tcgcattctg actggttgcc tgtcagaggc ggagaatctg gtgattttgt
     2881 ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca cctgcttccc gccgcggtga
     2941 gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt cgaggtgtgg cttgccccga
     3001 ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg gtgataacgg caattccggg
     3061 agtaccggcg gctgatctgt ctggagcgga tttgctcaaa gcgtggccgt caatggggca
     3121 gcaacttggc gctgttcaca gcctatcggt tgatcaatgt ccgtttgagc gcaggctgtc
     3181 gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat gccgtcaatc ccgacttctt
     3241 accggacgag gacaagagta cgccgcagct cgatcttttg gctcgtgtcg aacgagagct
     3301 accggtgcgg ctcgaccaag agcgcaccga tatggttgtt tgccatggtg atccctgcat
     3361 gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg accttgggcg
     3421 gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg ccgaagagaa
     3481 ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg tattggggat
     3541 cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc tgacttgggg
     3601 ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc tgttctcatt
     3661 gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac tcctgcaatc
     3721 gtcaagggat tgaaacctat agaagacatt gctgatgaac tgcgcggggc cgactatctg
     3781 gtatggcgca atgggagggg agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg
     3841 ctcgaatatg ccggggagcg aatgctctct cacatcgttg ccgagcacgg cgactaccag
     3901 gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg ccgcatctga ggaacccctg
     3961 ccttctgccc ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat
     4021 gatcaaaacg caggttgtca aactgactac gtccacgcgg cgattatagc cgatcaaatg
     4081 atgagcaatg cctcggaact gcgtgggcta catggcgatc tgcatcatga aaacatcatg
     4141 ttctccagtc gcggctggct ggtgatagat cccgtcggtc tggtcggtga agtgggcttt
     4201 ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct cgatcctaga
     4261 cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg
     4321 ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga tggagaagag
     4381 gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac gtcatactag
     4441 atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag aatatcgctg
     4501 gccaaagcct tagcgtagga ttccgcccct tcccgcaaac gaccccaaac aggaaacgca
     4561 gctgaaacgg gaagctcaac acccactgac gcatgggttg ttcaggcagt acttcatcaa
     4621 ccagcaaggc ggcactttcg gccatccgcc gcgccccaca gctcgggcag aaaccgcgac
     4681 gcttacagct gaaagcgacc aggtgctcgg cgtggcaaga ctcgcagcga acccgtagaa
     4741 agccatgctc cagccgcccg cattggagaa attcttcaaa ttcccgttgc acatagcccg
     4801 gcaattcctt tccctgctct gccataagcg cagcgaatgc cgggtaatac tcgtcaacga
     4861 tctgatagag aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc
     4921 ggctggccgt cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac
     4981 atacagtcta tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct
     5041 agacattgcc agccagtgcc cgtcactccc actcttacat tgttcgcagt atctaggtac
     5101 atgttattgc gtgcaccttc atgatgtata atgttattaa tgacagcaga aagatcgaca
     5161 gaattaatat tcttcgtacc tgggacagtt atcgggtgtc ggccagtgat gagaccaaag
     5221 gatgcagtat aagatgaacc actactatta tcatggccac cattaccacc accgttaaaa
     5281 ctacctcccg aacttccaga gccgctatga ccatcattgt aggggtcatt tggtcccatg
     5341 tgaccaaaac caccgacagt accgctattt aactctgtac tcataaaatt atcctctttg
     5401 acttttaaaa acagcaaaaa ctgtatttat aacaatgtgt ttatatacag tattcgaaat
     5461 actacgcccg tcaaaaccac accgtcaaca ggttttttgt gtatttttca cgcctcacgg
     5521 gcagcctcag accttttgtg ttaaaacagt aatatgttct gctgttacaa tacggtacac
     5581 acaggagaca ctgtcacttt tatggcatgg cacacagttc aagagacact gaaattaaca
     5641 ggaaagtcgc gctcgcaatt atatcgtgac atggctaaag gccttgtcag ctatcgcaca
     5701 ggaaaaaatg atcgccggga gttcgagacc agtgaactga ttcgggccta tggcgagctg
     5761 cgcacttctg agacaccaaa aaggcacaag gagggacact ccaaatctgt cgttagccag
     5821 gcggaaaatg ctcagctaga cgtcattcag cagaaactgg acgctctgca gcagactgtt
     5881 accctaatgc ttgaagacaa gatggcgcgg gaagcagaaa accgcctgcg aaatgaggag
     5941 agggaacaac tacaggccga aattagccgt ctggctgacg cgctggcaca ggaaaaaaag
     6001 cggggattct ggtcgcggct gtttcgctcc ggttcagagt aacagtgcag gtgactctga
     6061 tttaccataa catctgttat cagcaccgcc tgagaggccc tcagggcgtt tctgacggga
     6121 aaccggtaaa acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt
     6181 ttacggcatg catgagtatg
//
