LOCUS       NZ_CP074119            10060 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain kpn-hnqyy plasmid
            unnamed3, complete sequence.
ACCESSION   NZ_CP074119
VERSION     NZ_CP074119.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18920621
            Assembly: GCF_018314145.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Xiao,W. and Qu,Y.
  TITLE     The character of NDM-5 and KPC-2 co-producing Klebsiella pneumoniae
            from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Xiao,W. and Qu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2021) Department of Clinical Laboratory,
            Affiliated Cancer Hospital of Zheng Zhou University, NO.127 Dongming
            Road, Zheng Zhou, Henan 450008, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. V0.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.95x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 10:46:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,765
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,499
            CDSs (with protein)               :: 5,499
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 52 of 143
            Pseudo Genes (incomplete)         :: 93 of 143
            Pseudo Genes (internal stop)      :: 14 of 143
            Pseudo Genes (multiple problems)  :: 16 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074119.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="kpn-hnqyy"
                     /isolation_source="Abdominal pus"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="unnamed3"
                     /geo_loc_name="China"
                     /lat_lon="34.16 N 112.42 E"
                     /collection_date="2021-01-06"
                     /collected_by="Zhengzhou Univercity"
     gene            1094..3058
                     /locus_tag="KGB32_RS28535"
                     /old_locus_tag="KGB32_28535"
     CDS             1094..3058
                     /locus_tag="KGB32_RS28535"
                     /old_locus_tag="KGB32_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3058..3789
                     /gene="mobC"
                     /locus_tag="KGB32_RS28540"
                     /old_locus_tag="KGB32_28540"
     CDS             3058..3789
                     /gene="mobC"
                     /locus_tag="KGB32_RS28540"
                     /old_locus_tag="KGB32_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3796..4326
                     /locus_tag="KGB32_RS28545"
                     /old_locus_tag="KGB32_28545"
     CDS             3796..4326
                     /locus_tag="KGB32_RS28545"
                     /old_locus_tag="KGB32_28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4353..4532)
                     /locus_tag="KGB32_RS28550"
                     /old_locus_tag="KGB32_28550"
     CDS             complement(4353..4532)
                     /locus_tag="KGB32_RS28550"
                     /old_locus_tag="KGB32_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4558..4986)
                     /locus_tag="KGB32_RS28555"
                     /old_locus_tag="KGB32_28555"
     CDS             complement(4558..4986)
                     /locus_tag="KGB32_RS28555"
                     /old_locus_tag="KGB32_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(5150..5464)
                     /locus_tag="KGB32_RS28560"
                     /old_locus_tag="KGB32_28560"
     CDS             complement(5150..5464)
                     /locus_tag="KGB32_RS28560"
                     /old_locus_tag="KGB32_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5452..5730)
                     /locus_tag="KGB32_RS28565"
                     /old_locus_tag="KGB32_28565"
     CDS             complement(5452..5730)
                     /locus_tag="KGB32_RS28565"
                     /old_locus_tag="KGB32_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5905..6270)
                     /locus_tag="KGB32_RS28905"
     CDS             complement(5905..6270)
                     /locus_tag="KGB32_RS28905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(6267..6638)
                     /gene="tolA"
                     /locus_tag="KGB32_RS28575"
                     /old_locus_tag="KGB32_28575"
     CDS             complement(6267..6638)
                     /gene="tolA"
                     /locus_tag="KGB32_RS28575"
                     /old_locus_tag="KGB32_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6912..7157)
                     /locus_tag="KGB32_RS28580"
                     /old_locus_tag="KGB32_28580"
     CDS             complement(6912..7157)
                     /locus_tag="KGB32_RS28580"
                     /old_locus_tag="KGB32_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7484..9169
                     /locus_tag="KGB32_RS28585"
                     /old_locus_tag="KGB32_28585"
     CDS             7484..9169
                     /locus_tag="KGB32_RS28585"
                     /old_locus_tag="KGB32_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9179..9436
                     /locus_tag="KGB32_RS28590"
                     /old_locus_tag="KGB32_28590"
     CDS             9179..9436
                     /locus_tag="KGB32_RS28590"
                     /old_locus_tag="KGB32_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9521..9670
                     /locus_tag="KGB32_RS28595"
                     /old_locus_tag="KGB32_28595"
     CDS             9521..9670
                     /locus_tag="KGB32_RS28595"
                     /old_locus_tag="KGB32_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2484..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2..292)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.611"
                     /coverage="100.0"
                     /evalue="4.16e-139"
     Typing          complement(9935..10060)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.425"
                     /coverage="98.0"
                     /evalue="1.85e-56"
     Typing          3059..3786
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gagataccag tgtgtgagct atgagaaagc gccacacttc ccgcaaggga gaacggcgga
       61 caggtatccg gtaaacggca gggtcggaac aggagagcgc aagagggagc gacctaccgg
      121 aaacggtggg gatctttaag tcctgtcggg tttcgcccgt actgtcagat tcatggttga
      181 gcctcacggc tcccacagat gcaccgaaaa agcgtctgtt tatgtgaact ctggcaggag
      241 ggcggagcct atggaaaaac gccaccggcg cggccctgcc gttttgcctc acatgttagt
      301 cccctgctta tccacggaat ctgtgggtaa ctttgtatgt gtccgcagcg cccgccgcag
      361 tctcacgccc ggagcgtagc gaccgagtga gcgaggaggc gcaatttccc cggcacctgc
      421 aagacggtca ccggacgcgc tgatgtggga tattgtcccc tgattaagtc tttctattcc
      481 ctgacgatca attatcagga taggtgaacc atgtcggttt ttcgctgaac attttacgtt
      541 aacctatctc tgttgctttt ttctccttgc ggagaaatgc aagtcatccc cgctggggcg
      601 tggctgactt caggggaaaa gaccttgctt acgctggctg gacgccaccg acgcacggtc
      661 ttttcccctc tttctggctc tgtccggctg gacgccggcc acgccagaaa ttcacccggc
      721 ccccgtcagg ctcacgcctg accacccaca aaaatccacg gtgctggacg cacctgacga
      781 agcagcaaag ctgctcctga ttttgcgggt ggtcagccgc tcgctatggc gaactctgac
      841 gggggccgga acaccggaaa agaccgcact ggggtgccgc cttttcctct cacacgctgg
      901 ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca tggggcggaa agaggctggc
      961 ctccgcggtg aaagctgaac gcaatcacca cggacgtgac cgccgccgcc atgccttggg
     1021 gcatgtccgc ggcttgtgca gcgggggctg cgcatcacgc cggtaaaccc gtcgtctgaa
     1081 ggagagagat tgcatgttta atacggattc gcttgcctgg cagtggcata tcccggagct
     1141 ggcccggcag atttacccgt gggtcagctg gctgtaccgg gagtattaca gtctggccgg
     1201 tttttttctg gttatcggcc tgtttttcgg tctggctgaa ctctgggccg gccgaatgcg
     1261 ttttgtgggg ctgagccata aggcgcggcg ggagtatgcc gatcgctata agcgcgataa
     1321 taaccggact gtgccatttt cggtgctggc ctcgctggat tcacccgggc ggatggtcag
     1381 ggcacggcga catgtctgga actacgcgta ctggctgacc ggcgctgccg gtctggccct
     1441 tctggggggg ctggttgtcg ccgtgtttat cgagagctgg ggagaaaccg tcctgcgccc
     1501 ggacctctat gcgaacgcag attattacct gctgcagccg gctaacctgt tcgggatagc
     1561 ctggtgtgcc ggagggcttg tcgccggcgt ccttctggcg cggctgctgg cgttcctgct
     1621 gcttgatggt cattttgcgg ccgctgacga ggcggtgaac gcgaaactga atcaggagag
     1681 ccggcgcagc agccagcgga ccggagaaat gacagacgtc cgccacctgc atttcgggga
     1741 gccggtaccg gttaacgcgc tggccgattt ttccacggag caggcccgga aacagcaggc
     1801 ggtgtttctg ggtaaggatg agcagggcca gccggtgctg gtccctcgtg atacctggcg
     1861 caagaccaat attcagatcc tcggcctgcc cgggagcggc aagagcgcga tggccaccaa
     1921 cgcgctcatt cgctgcgtac gcgatttcgg ggacgctgtg gtgtacttcg accccaaagg
     1981 ggacgcgtgg gcgccgcacg tcttccgggc gcactgcccg gacttcaccc tgctggatct
     2041 gcggccgggg aaaccggcgc agctgaatct gttccgcgat ctcgaccagt acgccctgaa
     2101 gaatctgctg gttgccgggt ttaacctgag tgagaccggc gacgtggcgg accactaccg
     2161 catcagcgag cagaaagcgg cgaagctgat tgcggagcag ttcccgcagg gggcaaacat
     2221 tcagcaggtg ctggcggcgg cgtacgcgtt gccggaggcc ctgaaaaagg acgtgaaggg
     2281 gctgatcacc aagctggaga acgtggccga cctgagcgtc ctgcagacgg acagcggtat
     2341 cgatgtggcg gggataatca acggcggcgg ctgcctgtac gtcatcggct cgatggacga
     2401 tgaggcggtg atccgggtgc agaagatgct gtttgcccgc tgcgcgcaaa tcatcattgc
     2461 gcgggatgag ttccggaggt ggccgcacgc gagcatcatg ctcgatgaga ttaaatatct
     2521 gctgtcgaag tacgtgctga acgcgctggg gacgctgcgc agccgggact gcaacctgct
     2581 gctcgcgcac cagtcgctgg gcgacttcgg tcagtgcggg caggatctgc ccgctgattt
     2641 tgtgaaaacc acggtactgg ataacacgcc gatccgctgg ttctatcgcg cggccagcca
     2701 ggagtccgcg cagtgggcgg cgggccagac cggggagatc cgggtggacg tggagcgccg
     2761 ccgggccagc cgggaggcgg ggaacgtgga gcacatcagc ggcgacagct tcatccagaa
     2821 ggaggcgcgg ccgctgtttg acgtgaacac cctgcaacac ctgcctgacg ggttcgcggt
     2881 gatgaccggg ctgggcgtgg cgcggctggc gttcagcagc ccgctgcggg ttgagcgccg
     2941 ggaaatttcg ctgaaaacct tcccggtgct ggcgaaaacg gacccgctgg cggagtacct
     3001 gcctccggcc gacggcggcg gaccaaaagc ggatgatgat tttgcggggc tttactgatg
     3061 acactggagc agtacaacgt cagccacgcg aagcggcagg cgcggaacgc ggagaaaaca
     3121 cggctgaccc tgcgctggct gcgggaagag ctgtgttcca cggcggagct ggttgcgcga
     3181 cggctgggga ttgcggcggt acagccggtt taccgcttcc tggacagtct ggtggcgaaa
     3241 gggctgctgg tccgggcgaa atacccggtt gacggccgtc aggtcagcgt gtgggggctg
     3301 acgccgcacg gggtggcgtt ctcgttcgac gaggacgagc cgctgacgga cgttattccg
     3361 tttcagccgt caagggtgtc ggcggcgcag ctgccgcacc ggctggccgt gcagtccctg
     3421 cgcctctcca tggagggccg gggggccacc ggctggcggt acctgcaccg tatggccctg
     3481 aaagggatga aagtgccgga cgcgctggcg gagctggacg gcagaacggt ggcatttgag
     3541 gtagagcgca ccgtcaagag ccgcaggcgg tatcaggaag tggtgtccgg gtatctgttc
     3601 aaccgccggg cgaacggcat cgatgaaatc tggtacatct gccctgaccg gccgacgctt
     3661 gtccgggtac agcgggcgat tctggcggtg gatgaaatcg tgaatccaca gaccggcgag
     3721 gcccgcaaaa cggtggagct ggaccgggag aggctgttcg cgtgctttaa atttatgaca
     3781 acggagtgag ggattatggc gctgtacatg gtatttattg aggacgtgga agacaaagac
     3841 ggcgagcgct gcattatgcc aaccagcgag catctggaag acatggtggt gctcgcctgg
     3901 cagaccgggg cgtatgtttt ctgttttgtc gaccgggccc agcatctggc gatgatgaaa
     3961 tccatcggga tgtatgacga gcggctcaac agcgacgaaa aagccacgta ctttttcacc
     4021 ggtgaaaatc cgcaatggga acgggacaaa cccacaggaa tgaagctgtg tgattacctc
     4081 tatcgaaagg tggagcggtt cgccatcgag aacggtgtgc aggtggttga tgccgggaag
     4141 tgctcaccgg agcagattaa cgcgatgatt gaggtggtca gcgagcggca cggggaaagc
     4201 gcggtgaacg agaagctgaa agcggagctg atgcgggaat ataacagccg ctattttaac
     4261 gtgccggtgc ctgaactgcc ggaggacaaa cccgcccgcc ggtggtggcc gttcggcaaa
     4321 aaataacgcc agcaccggca tggccggtgc tgttattctt ccccgagctt atcccgcatc
     4381 acctgccaga gtgtttccgc atcctcgtga agccgttcgc acatgtcggc cggggtatcc
     4441 atgtccagct cgttcagctt ttccagcaac agcagcgact gatccttaat gaatttagcc
     4501 atattgaggg cagttttttc ctgcttcgtc atgttctcca ttcccttcgg taagttctca
     4561 gctaaaccag ccggaccaga agcgtttttt cttctgtgtc agttctgttg tcaatctgtc
     4621 cacttcggcc tggagtttcc ggcgctcatc atcgtgctgg cggcgttctt cttcccggga
     4681 tgttttatct tccagcatca gggtgacagc ctgccggaga tcggccagtt ccttttgcat
     4741 tgccagaagc tgttcctgtg ggaaattttt ttgtgtattt tcacggtgtg aaatgccggg
     4801 aatttgtttc tcagggatat tcacatttcc gtatacacgg atcagttctg acacatccac
     4861 gacagggttg tttttcccgt cacgtgacac ggtaaccttt ccctgtttta catgattgta
     4921 tagcgttctt ctggtaatgc ccgcggcttg tgctgcctgt gaaaggttga gcaatgtttt
     4981 cgccactgta tacccctgat tttatcgggt gtgaaaggtg tgtaatttct cacggtaaat
     5041 gtatcgctga attttacccg tttttccgtt caggatgtgg ttttctgacg cgctgcgctt
     5101 gtcaaaccgg cgacggccac gcaatgaatt gcgcccccgt catcggttat tagaggctgt
     5161 ctgcccccgg atacccggat tctttctgat gcctgacggt gacgatcaca atctggtcca
     5221 tttcccggtc gtggcggtag agcatcacat agccactgtc gccaaacccg atcaccagtt
     5281 ccttatattc cagcggcaga aacggcaccg ggcggccaat gtctggcaat gttttcagtt
     5341 gctggatagc ccggacgatc acctcacctc cttttctggc tgccagccga tttttggttt
     5401 tgagaaagtc ctgaaggcgt tgtaaatcgc gctgtgccag cgcggaaatc gttacctgtg
     5461 gcatggcgga gcatcctgct cgttctcagt tccccaggta cttatccagg cttccgcttc
     5521 ttccgcagta aggtgcagac ccgtttcctg atagtgctgc catgcggcct gtccgtcccg
     5581 caggtactgg ctgcgcttct cttcccggtc gatgtattcg gtgattgcct ccagcattag
     5641 cgcgtgcgct gagcggtgcc ggtcatcggc cagcgttttc agccggtcct taagttcttc
     5701 atcgagcctg atggatgttg ctgatgacat aaaagcccct ttgtagtcat atgtattaca
     5761 catgactact ttaatccgtg gcggcgctat ttgccagaaa tacggctttt gctgcccatt
     5821 tttcagcttc gcttccacta atcagggctg ctacaggctc actcatcatc caggactatt
     5881 gcagcctgcg ggttaaagga cctgttactg gcggcaacca tcatcattga gcttttgggt
     5941 ggtgactggt cgcggatcct gtaagccgtc atttttcaga ctgaattcaa aatttataac
     6001 ctgtcgcatt gccgggtgcc ctgcgggata tagccagtgc ctcattgccg tcagcgtttc
     6061 ccgaaaaaaa aggtgacagc cggtaagcga cacgttgtcc actcgtccat cgtccagaat
     6121 atcaaacgtg gcggtcacct gaccttcgat gcgttttgct gcggcctgta ccgggtaggt
     6181 tggctgcagt gtggttacgg gttttagtgg tatcgcatgt accggagtga ggaacatggc
     6241 cgccagcact gtcagagtaa aagctttcat ggtctgaaat ccagaggggc gtttttgaat
     6301 atttgccagg tattttcatc cggtgccggc ggaattttgg cgtgggtaac ggctgatatt
     6361 gctgctttac agagtttcgg atctcctcct tcagcaatgg cgctgttcag agtgccatca
     6421 cgttgtatgg atatccgcac ggtgcacatt tttcccgcat aggcctctgg ttcaaacatc
     6481 tgcatttcaa cagcggtctt gatggccgca gcgtagctac tgattttaga tgacttatcc
     6541 agagtataaa ttttcggcgg ctgggtggtg gaatgcttac aggccgtcag gagtaagggg
     6601 ctgcacaatg ccagcaggat aaagggtatt aaacgcatcg ggttgatgac ctgtcagtaa
     6661 aaaatgttac atgtatattt gtcactcttt acggccacgt tcatttacat aaacacggct
     6721 tatcgtatcg tttttacacc aaacggcccg ccatgcagtt gcgtgcatag gcatgcagcg
     6781 ggttacaatt taccgggcgc gtttccggcg gttttccggg tggtttgttg cctgttttat
     6841 cccgtagccg ccggaaacgc cctcagaccg tctgagcggt gcgggcaatg cgacgttatg
     6901 gtaaataccc cttaccggcg gcgctgtggc tgccaggtga aggtggtttc cgtagcctcg
     6961 tatttctccc gacgcatttc ctgcgcccgc ctggcttcac gttcgtcctg aatggcacga
     7021 agcacccttt cccgcacctg ttgttttacc gcctttggca gcgttttgtt ccctttcagg
     7081 cccatggcca gtgcctgagc gacaaaggcg tccgccaggc gcttttcttc agcgggagtc
     7141 agtttcaggt gcgtcatagt ggcctccggg cgtctcaggt ttcccgaaca gtttaaccgc
     7201 aggtcctctc ctcatgccag ttagctttca gatgtgtcta taccatctga aatgtaagtg
     7261 tgatgaatgt cttaaataat aaaatttgtt gccatgagaa attttcggga aaagacgcga
     7321 tctgcttaca ggtggctccg ggcgcttttt tgtgcctgaa gatgagaact aatctcatat
     7381 attgacacct gaaaacggga ggagtaaggt aataattata ctgtgtatat gtacagtatg
     7441 ttggctttaa tttattgttt taaatgtcaa agaggaaaac ggtatgagtg gtggagacgg
     7501 tcgaggtccg ggtaattcag gtctgggaca taatggtggt caggccagtg ggaatgtgaa
     7561 cggtacgtct ggtaaaggtg gcccttcatc aggtggtggt acggatccaa acagcgggcc
     7621 gggctggggt acgacgcata cgccgaatgg agatattcat aactataatc cgggggagtt
     7681 tggtaacggc gggagtaaac ccggtggtaa tggtggtaac agcggcaatc atagcggtag
     7741 ctctggtggc ggacagtctt cggccactgc gatggccttc ggtctgcctg ccctggctac
     7801 tccgggggct gaaggactgg ctttatcagt ttccggcgat gcgttgtcgg ccgccgttgc
     7861 tgatgtgctg gctgccctga aggggccgtt taagtttggt ctgtggggga ttgcgatcta
     7921 cggtgtgctg ccttctgaga ttgcaaaaga tgatccgaaa atgatgtcaa aaattatgac
     7981 gtcattaccg gccgatgcgg tgacggagac tccggcaagt actttaccac tggaccaggc
     8041 gacggttcgt gtcagacaac gggttgtgga tgtggtgaag gatgagcggc agcatattgc
     8101 ggttgtcgca ggtcggccaa tgagtgtccc tgtggtggat gcgaaaccga caaaacgtcc
     8161 gggggtattc agtgtgtcga ttccgggtct cccgtctctg caggtgagcg taccgaaagg
     8221 tgttccgaca gcgaaagccc cgccaaaagg cattgttgct gaaaaaggtg attcacgtcc
     8281 ggctggtttt acagccggtg gtaactcccg tgaggccgtt attcgtttcc cgaaagagac
     8341 cggacagaag ccggtttatg tgtcggtgac agatgttctt accccggcac aggtaaaaca
     8401 gcgtcaggag gaagaaaagc gtcgccagca ggcatgggac gccgctcatc cggaagaggg
     8461 gctgaaaaga gactatgata aagcgaaagc tgagctggat gccgaagata aaaatattgc
     8521 gaccttaaac agccgcattg catcgacaga gaaggcgctc cccggtgcaa gggctgctgt
     8581 tcaggaagcc gataaaaagg taaaagaggc agaggcaaat aaagatgatt ttgtgactta
     8641 taacccgcct catgaatatg gctccgggtg gcaggatcag gttcgctatc ttgataagga
     8701 tattcagaat cagaatgaga aattaaaagc ggctcaggca tctttaaacg caatgaatga
     8761 atccttatcc agagataagg ctgcacttac cggggcgatg gagagccgga aacaaaagga
     8821 gaaaaaagcg aaggatgcag aaaataagtt aaatgaggaa aagaacaagc ctcgcaaggg
     8881 gactaaagac tacggccatg attatcatcc agtacctaaa acagaagaca taaagggact
     8941 tggagagtta aagcgaggtg atcctaaaac acctaaacaa ggtggtggtg gtaagcgggc
     9001 ccgatggtat ggcgataaaa aacgtaaaat ttatgaatgg gattcccagc acggtgagct
     9061 tgaagggtat cgtgccagtg atggcgaaca cctaggcgca tttgatccca aaacgggtaa
     9121 gcaggttaaa gggccagatc caaaacggaa cattaaaaaa tatctttgag aggtaaatat
     9181 ggggcttaaa ttacgtttga catggtttga taagaaaact gaagagttta aaggggaaga
     9241 gtattctaaa gactttggtg acgatggttc tgtcattgaa agcttgggaa tgcctttaaa
     9301 ggataatatt aataatggtg agtttgattt gaagaaagaa tgggttcctt tactacagcc
     9361 attttttaaa aatcaaataa aacctgatgt ggataattgt tatatagctt ttgattatcg
     9421 ggacggtaac tggtaacaaa aaattatcaa ggatgatttg aaatactgac ttccggtgtt
     9481 cagggatgaa tgccggagtc tttaaataca gggaaagtat atgaaaaagg taaagactat
     9541 ttttcttttt atattgattg ttgctggctt tcttctggcc gcatgtcagg caaattatat
     9601 ccgggatgtt cagggcggaa cggtggcacc atcgtcctcc tctgaactga cggggatcgc
     9661 ggttcagtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatcttt
     9721 tgccctgtaa acgaaaaaac cacctgggga ggtggtttga tcgaaggtta agtcagttgg
     9781 ggaactgctt aaccgtggta actggctttc gcagagcaca gcaaccaaat ctgtccttcc
     9841 agtgtagccg gactttggcg cacacttcaa gagcaaccgc gtgtttagct aaacaaatcc
     9901 tctgcgaact cccagttacc aatggctgct gccagtggcg ttttgcgtgc tttcccgggt
     9961 tggactcaag tgaacagtta ccggataagg cgcagcagtc gggctgaacg gggagttctt
    10021 gcttacagcc cagcttggag cgaacgacct acaccgagcc
//
