LOCUS       NZ_CP074037             6647 bp    DNA     circular CON 24-AUG-2023
DEFINITION  Escherichia coli strain PI7 plasmid pYLPI7d, complete sequence.
ACCESSION   NZ_CP074037
VERSION     NZ_CP074037.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18877292
            Assembly: GCF_024585925.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Lopez-Dorta,Y., Brilhante,M., Hernandez-Fillor,R.E., Baez,M.,
            Espinosa,I. and Perreten,V.
  TITLE     Molecular epidemiology of Extended-Spectrum Beta-Lactamase-producing
            Escherichia coli from food-producing animals in Cuba using
            whole-genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Lopez-Dorta,Y., Brilhante,M. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2021) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8; Canu v. 2.1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/24/2023 07:00:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,135
            CDSs (total)                      :: 5,008
            Genes (coding)                    :: 4,808
            CDSs (with protein)               :: 4,808
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 64 of 200
            Pseudo Genes (incomplete)         :: 130 of 200
            Pseudo Genes (internal stop)      :: 42 of 200
            Pseudo Genes (multiple problems)  :: 35 of 200
            Pseudo Genes (short protein)      :: 3 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074037.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PI7"
                     /isolation_source="Rectal Swab"
                     /host="Pig"
                     /db_xref="taxon:562"
                     /plasmid="pYLPI7d"
                     /geo_loc_name="Cuba: San Jose de Las Lajas"
                     /collection_date="2018-05"
                     /genotype="ST162"
                     /note="Post-weaning piglet"
     gene            308..466
                     /locus_tag="KFU70_RS25690"
     CDS             308..466
                     /locus_tag="KFU70_RS25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            964..1191
                     /locus_tag="KFU70_RS25435"
                     /old_locus_tag="KFU70_25220"
     CDS             964..1191
                     /locus_tag="KFU70_RS25435"
                     /old_locus_tag="KFU70_25220"
                     /inference="COORDINATES: protein motif:HMM:NF015489.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(1188..2756)
                     /gene="cea"
                     /locus_tag="KFU70_RS25440"
                     /old_locus_tag="KFU70_25225"
     CDS             complement(1188..2756)
                     /gene="cea"
                     /locus_tag="KFU70_RS25440"
                     /old_locus_tag="KFU70_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            3016..3453
                     /locus_tag="KFU70_RS25445"
                     /old_locus_tag="KFU70_25230"
     CDS             3016..3453
                     /locus_tag="KFU70_RS25445"
                     /old_locus_tag="KFU70_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3534..3953
                     /locus_tag="KFU70_RS25450"
                     /old_locus_tag="KFU70_25235"
     CDS             3534..3953
                     /locus_tag="KFU70_RS25450"
                     /old_locus_tag="KFU70_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4271..4504)
                     /locus_tag="KFU70_RS25455"
                     /old_locus_tag="KFU70_25240"
     CDS             complement(4271..4504)
                     /locus_tag="KFU70_RS25455"
                     /old_locus_tag="KFU70_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4511..5029)
                     /locus_tag="KFU70_RS25460"
                     /old_locus_tag="KFU70_25245"
     CDS             complement(4511..5029)
                     /locus_tag="KFU70_RS25460"
                     /old_locus_tag="KFU70_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5067..5765)
                     /locus_tag="KFU70_RS25695"
                     /old_locus_tag="KFU70_25250"
                     /pseudo=""
     CDS             complement(<5067..5765)
                     /locus_tag="KFU70_RS25695"
                     /old_locus_tag="KFU70_25250"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5707..6030)
                     /locus_tag="KFU70_RS25470"
                     /old_locus_tag="KFU70_25255"
     CDS             complement(5707..6030)
                     /locus_tag="KFU70_RS25470"
                     /old_locus_tag="KFU70_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6094..6285
                     /locus_tag="KFU70_RS25475"
                     /old_locus_tag="KFU70_25260"
     CDS             6094..6285
                     /locus_tag="KFU70_RS25475"
                     /old_locus_tag="KFU70_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6371..6458)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          10..417
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.088"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6469..6647
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.818"
                     /coverage="100.0"
                     /evalue="2.64e-66"
     Typing          complement(4168..5717)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagaacatgt gagcaaaaga ccaggaacag gaagaaggcc acgtagcagg cgtttttcca
       61 taggctccgc ccccctgacg agcatcacaa aaatagacgc tcaagtcaga ggtggcgaaa
      121 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      181 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      241 gctttctcat agctcacgct gttggtatct cagttcggtg taggtcgttc gctccaagct
      301 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      361 tcttgagtcc aacccggtaa ggcacgcctt aacgccactg gcagcagcca ctggtaaccg
      421 gattagcaga gcgatgatgg cacaaacggt gctacagagt tcttgaagta gtggcccgac
      481 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgttggtag cggtggtttt
      601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttaatc
      661 ttttctactg aaccgcgatc cccgtcagtt tagaagagga ggatggtgcg atggtccctc
      721 cctgaacatc acgtatatag ttagcctgac atccaacaag gaggtttatc gcgaatattc
      781 ccacaaaaaa tcttttcctc ataactcgat ccttataaaa tgaaaagaat atatggcgag
      841 gtttaattta tgagcttaag atactacata aaaaatattt tatttggcct gtactgcaca
      901 cttatatata tataccttat aacaaaaaac agcgaagggt attatttcct tgtgtcagat
      961 aagatgctat atgcaatagt gataagcact attctatgtc catattcaaa atatgctatt
     1021 gaatacatag cttttaactt cataaagaaa gattttttcg aaagaagaaa aaacctaaat
     1081 aacgcccccg tagcaaaatt aaacctattt atgctatata atctactttg tttggtccta
     1141 gcaatcccat ttggattgct aggacttttt atatcaataa agaataatta aatccctaac
     1201 acctcattta tagtattaag tttattctta tcaatatagg agcatagaat tcctgtaaca
     1261 atagcaatac cccaaatacc taatgtagtt ccagcaagca agctaaaaag taaagcaaca
     1321 acataactca cccctgcatc tgcagctttc ttctctaatg taagaaatag tggcttccag
     1381 tcacctgtat ccttaatttt taggatatca gataccacat catatccaaa agaaacatgc
     1441 cccgtaatct tcaagtactt ggcaaactga tctaaatgtt tagcccagtc atcatacttc
     1501 accgatgcca acgcattaaa aatagcatca cgatcggctt tgctgaattt cttatttaaa
     1561 acatccttgt atttttcaaa agcagcgaga gcttcattca cattgccgat tttcttacct
     1621 ttagacttat cagcaagttc ctgtgccatt ttcgaatatt tttcaccata tttttcagtc
     1681 agcgtttgat aaaagctaac tgttgcatca acagcatcct taatctgtga attaaggaga
     1741 ttattctgtg cttttttcaa attttcttca gcttcatgaa cacgagcgat accggcatta
     1801 cgattattac tgacctgaga aatagccttc tggatctgag ttatatcagc atttatccgg
     1861 ttaatacgtg tttctgatgc tgttacctgt ttttgttttt cttctctaat cttaccggcc
     1921 ccaacccgtc gtctggttgc ttcaaaaaaa ggacggttct gaagcggatc attggctctt
     1981 ggtgatagtt ttttgaccag ctcatccagt tctttatatt tagcggatgc ctgagccagt
     2041 tcatttcgtt ttccagcgag cgttttcatt tctgcatcac gggcatggat actggagctt
     2101 aaacgagaat tgagagtctt aatctctcca tccattttca ccacttcaga ttgtgcagca
     2161 gaaagttttt tttgggcgat ctcaacagct ttagcttctt cactcaatgc agccagtcgt
     2221 ttctcttcag cttcagccag tttcaactgg cgttctgttt cagccttctc ccgttcaatc
     2281 tctttacgtc gttgttctgc ttcctgaaaa gccttttctg ctgcttccgc ttctttacgg
     2341 gctttttctt ctgctttcgc aaggcgcaaa cgctctgctt ccgcctgcat agctgcatta
     2401 ttagcatgag caagctctgt tgctgaaggc gtacgtgagg cattgtgacg aagagcctca
     2461 ttcacgatat ccttcaggcg ctgagtcagc gcatccctgt ttgcctttgc tttcgcctgt
     2521 gcttccgctg cagcttttgc ccgggcagcc tgctctgcct gtgttttctt taattgagca
     2581 gtagaccatt tagcagttgc atgaatagct gcagaacttt cacttttact gcctcctttt
     2641 ccacctccgc cgccagagcc actcccgtca ggagtaccat tcaaaagagt aataattacc
     2701 tgtcccttat catcataagg aacaccatct ttatagtacg ctaccgcggt ttccattata
     2761 aaatcctctt tgacttttaa aacaataagt taaaaataaa tactgtacat ataaccactg
     2821 gttttatata cagcataaaa gctacgccgc tgcattttcc ctgtcaagac tgtggacttc
     2881 catttttgtg aaaacgatca aaaaaacagt ctttcacacc acgcgctatt ctcgcccgat
     2941 gccacaaaaa ccagcacaaa cattaccgtt ctcagacctc attatgtttt actgaaacta
     3001 tgagatgaga catctatggg acactgtcac tttatggcat ggcacacact ccgggacgca
     3061 ctaaaaatga caggcagatc gcgttcacag ttttaccgtg atatgcgcgc aggccttgtc
     3121 agttaccgta ccggcaggga cggacgacgg gagtttgaaa ccagtgaact gatccgggca
     3181 tacggcgaat taaagcagaa tgagacacca gaaaggcaca gtgagggaca tgcagaaaat
     3241 ccacatgatc agcagacaga acgcattctc cgggaactga atgagctgaa acaatgcctg
     3301 acgctgatgc ttgaggataa acaggcacag gatatggatc gcagacgcca ggaagcagaa
     3361 cgggaacagc tacaaaatga gatagcccag ctcaggcagg cactggaact ggaaaagaaa
     3421 cggggattct ggtccaggtt gttcggtcgc tgaacgctgt cagagactga tgataaaata
     3481 gtcttcggat aataactcac cgagaataaa tactttaagg tagggagaca ctcatgagac
     3541 gtaccggaaa caaactttgt cttatcgcca tgataacagc aacagtagct ctcacagcct
     3601 gtaccccaaa gggcagcgtg gaacaacata cccggcatta cgtatatgct tctgatgacg
     3661 gttttgatcc caacttttcc acccaaaaag ccgacacaac acgaatgatg gtgccttttt
     3721 ttcggcagtt ctgggatatg ggagctaaag ataaagcgac aggaaaatca cggagtgatg
     3781 tgcaacaacg cattcagcag tttcacagcc aggaattttt aaactcactc cggggcacaa
     3841 ctcaatttgc gggtactgat taccgcagca aagaccttac cccgaaaaaa tccaggctgc
     3901 tggctgacac gatttctgcg gtttatctcg atggctacga gggcagacag taagtggatt
     3961 taccataatc ccttaattgt acgcaccgct aaaacgcgtt cagcgcgatc acggcagcag
     4021 acaggtaaaa atggcaacaa accacccgaa aaactgccgc gatcgcgcct gataaatttt
     4081 aaccgtatga atacctatgc aaccagaggg tacaggccac attaccccca cttaatccac
     4141 tgaagctgcc atttttcatg gtttcaccat cccagcgaag ggccatccag cgtgcgttcc
     4201 tgtatttccg gctgacgctc ccgttccagg gataacacat gttcgcgctc ctgtatcagc
     4261 cgttcctctc ttatctccag ttctcgctgt ataactggct caagcgttct gtctgctcgc
     4321 tcaagtgctg cacctgctga ctcaactgca tgacccgctc gttcagcatc gcgttgtccc
     4381 gttgcgtaag cgaaaacatc ttctgcaatt ccacgaaggc gctctcccat tcgctcagcc
     4441 gctgcatata gtcctgttgc agctgctcta aggcgttcag caaatgtgtt tccagctctg
     4501 tcactctgtg tcactccttc agatgtaccc actctttccc ctgaaaggga atcacctccg
     4561 ctgatttccc gtacggaagg acaaggaatt tcctgttccc gtcctgcaca aactccacgc
     4621 cccatgtctt cgcgttcagt ttctgcaatg tctcttcctg cttcctgatt tcttccaggt
     4681 tcgcctgtat cctccctcca agataccaga gcgtcccgcc actcgcggta aacaggagaa
     4741 agactatccc cagtaacatc atgcccgtat tccctgccag ctttaacacg tccctcctgt
     4801 gctgcatcat cgcctctttc accccttccc ggtgtttttc cagcgattcc tctgtcgagg
     4861 ctgtgaacag ggctatagcg tctctgattt tcgtctcgtt tgatgtcaca gcctcgctta
     4921 cagattcgcc gagcctcctg aactcgttgt tcagcatttt ctctgtagat tcggctctct
     4981 ctttcagctt tttctcgaac tccgcgcccg tctgcaaaag attgctcata aaatgctcct
     5041 ttcagcctga tattcttccc gccgttcgga tctgcaatgc tgatactgct tcgcgtcacc
     5101 ctgaccactt ccagccccgc ctcagtgagc gcctgaatca catcctgacg gccttttatc
     5161 tctccggcat ggtaaagtgc atctatacct cgcgtgacgc cctcagcaag cgcctgtttc
     5221 gtttcaggca ggttatcagg gagtgtcagc gtcctgcggt tctccggggc gttcgggtca
     5281 tgcagcccgt aatggtgatt taccagcgtc tgccaggcat caattctagg cctgtctgcg
     5341 cggtcgtagt acggctggag gcgttttccg gtctgtagct ccatgttcgg aatgacaaaa
     5401 ttcagctcaa gccgtccctt gtcctggtgc tccacccaca ggatgctgta ctgatttttt
     5461 tcgagaccgg gcatcagtac acgctcaaag ctcgccatca ctttttcacg tcctcccggc
     5521 ggcagctcct tctccgcgaa cgacagaaca ccggacgtgt atttcttcgc aaatggcgtg
     5581 gcatcgatga gttcccggac ttcttccgga ttaccctgaa gcaccgttgc gccttcgcgg
     5641 ttacgctccc tccccagcag gtaatcaacc ggaccactgc caccaccttt tcccctggca
     5701 tgaaatttaa ctatcatccc gcgccccctg ttccctgaca gccagacgca gccggcgcag
     5761 ctcatccccg atggccatca gtgcggccac cacctgaacc cggtcaccgg aagaccactg
     5821 cccgctgttc accttacggg ctgtctgatt caggttattt ccgatggcgg ccagctgacg
     5881 cagtaacggc ggtgccagtg tcggcagttt tccggaacgg gcaaccggct cccccaggca
     5941 gacccgccgc atccataccg ccagttgttt accctcacag cgttcaagta accgggcatg
     6001 ttcatcatca gtaacccgta ttgtgagcat cctctcgcgt ttcatcggta tcattacccc
     6061 atgaacagaa atccccctta cacggaggca tcagtgacta aacaggaaaa aaccgccctt
     6121 aacatggccc gctttatcag aagccagaca ttaacgctgc tggagaagct caacgaactg
     6181 gacgcagatg aacaggccga tatttgtgaa tcgcttcacg accacgccga tgagctttac
     6241 cgcagctgcc tcgcacgttt cggggatgac ggtgaaaacc tctgacacat gcagctcccg
     6301 gagacggtca cagcttgtct gtgagcggat gccgggagct gacaagcccg tcagggcgcg
     6361 tcagcaggtt ttagcgggtg tcggggcgca gccctgaccc agtcacgtag cgatagcgga
     6421 gtgtatactg gcttaaccat gcggcatcag tgcggattgt atgaaaagta cgccatgccg
     6481 ggtgtgaaat gccgcacaga tgcgtaagga gaaaatgcac gtccaggcgc ttttccgctt
     6541 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggta ctgactcaca
     6601 caaaaacggt aacacagtta tccacagaat caggggataa ggccgga
//
