LOCUS       NZ_CP074023             6647 bp    DNA     circular CON 24-AUG-2023
DEFINITION  Escherichia coli strain PM22 plasmid pYLPM22d, complete sequence.
ACCESSION   NZ_CP074023
VERSION     NZ_CP074023.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18877296
            Assembly: GCF_024585845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Lopez-Dorta,Y., Brilhante,M., Hernandez-Fillor,R.E., Baez,M.,
            Espinosa,I. and Perreten,V.
  TITLE     Molecular epidemiology of Extended-Spectrum Beta-Lactamase-producing
            Escherichia coli from food-producing animals in Cuba using
            whole-genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Lopez-Dorta,Y., Brilhante,M. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2021) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8; Canu v. 2.1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/24/2023 06:55:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,931
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,639
            CDSs (with protein)               :: 4,639
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 64 of 172
            Pseudo Genes (incomplete)         :: 101 of 172
            Pseudo Genes (internal stop)      :: 36 of 172
            Pseudo Genes (multiple problems)  :: 30 of 172
            Pseudo Genes (short protein)      :: 3 of 172
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PM22"
                     /isolation_source="Rectal Swab"
                     /host="Pig"
                     /db_xref="taxon:562"
                     /plasmid="pYLPM22d"
                     /geo_loc_name="Cuba: La Lisa"
                     /collection_date="2018-05"
                     /genotype="ST2599"
                     /note="Fattening pigs"
     gene            complement(103..522)
                     /locus_tag="KFU87_RS24510"
                     /old_locus_tag="KFU87_24355"
     CDS             complement(103..522)
                     /locus_tag="KFU87_RS24510"
                     /old_locus_tag="KFU87_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(603..1040)
                     /locus_tag="KFU87_RS24515"
                     /old_locus_tag="KFU87_24360"
     CDS             complement(603..1040)
                     /locus_tag="KFU87_RS24515"
                     /old_locus_tag="KFU87_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1300..2868
                     /gene="cea"
                     /locus_tag="KFU87_RS24520"
                     /old_locus_tag="KFU87_24365"
     CDS             1300..2868
                     /gene="cea"
                     /locus_tag="KFU87_RS24520"
                     /old_locus_tag="KFU87_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(2865..3206)
                     /locus_tag="KFU87_RS24525"
                     /old_locus_tag="KFU87_24370"
     CDS             complement(2865..3206)
                     /locus_tag="KFU87_RS24525"
                     /old_locus_tag="KFU87_24370"
                     /inference="COORDINATES: protein motif:HMM:NF015489.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058760.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            3254..3391
                     /locus_tag="KFU87_RS24530"
                     /old_locus_tag="KFU87_24375"
     CDS             3254..3391
                     /locus_tag="KFU87_RS24530"
                     /old_locus_tag="KFU87_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3590..3748)
                     /locus_tag="KFU87_RS24695"
     CDS             complement(3590..3748)
                     /locus_tag="KFU87_RS24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(4418..4609)
                     /locus_tag="KFU87_RS24535"
                     /old_locus_tag="KFU87_24380"
     CDS             complement(4418..4609)
                     /locus_tag="KFU87_RS24535"
                     /old_locus_tag="KFU87_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4673..4996
                     /locus_tag="KFU87_RS24540"
                     /old_locus_tag="KFU87_24385"
     CDS             4673..4996
                     /locus_tag="KFU87_RS24540"
                     /old_locus_tag="KFU87_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5205..>5636
                     /locus_tag="KFU87_RS24545"
                     /pseudo=""
     CDS             5205..>5636
                     /locus_tag="KFU87_RS24545"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5674..6192
                     /locus_tag="KFU87_RS24550"
                     /old_locus_tag="KFU87_24395"
     CDS             5674..6192
                     /locus_tag="KFU87_RS24550"
                     /old_locus_tag="KFU87_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            6199..6432
                     /locus_tag="KFU87_RS24555"
                     /old_locus_tag="KFU87_24400"
     CDS             6199..6432
                     /locus_tag="KFU87_RS24555"
                     /old_locus_tag="KFU87_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          4246..4333
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(3677..4232)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4987..6536
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtttttcgg gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc
       61 gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta
      121 gccatcgaga taaaccgcag aaatcgtgtc agccagcagc ctggattttt tcggggtaag
      181 gtctttgctg cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg agtttaaaaa
      241 ttcctggctg tgaaactgct gaatgcgttg ttgcacatca ctccgtgatt ttcctgtcgc
      301 tttatcttta gctcccatat cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt
      361 gtcggctttt tgggtggaaa agttgggatc aaaaccgtca tcagaagcat atacgtaatg
      421 ccgggtatgt tgttccacgc tgccctttgg ggtacaggct gtgagagcta ctgttgctgt
      481 tatcatggcg ataagacaaa gtttgtttcc ggtacgtctc atgagtgtct ccctacctta
      541 aagtatttat tctcggtgag ttattatccg aagactattt tatcatcagt ctctgacagc
      601 gttcagcgac cgaacaacct ggaccagaat ccccgtttct tttccagttc cagtgcctgc
      661 ctgagctggg ctatctcatt ttgtagctgt tcccgttctg cttcctggcg tctgcgatcc
      721 atatcctgtg cctgtttatc ctcaagcatc agcgtcaggc attgtttcag ctcattcagc
      781 tcccggagaa tgcgttctgt ctgctgatca tgtggatttt ctgcatgtcc ctcactgtgc
      841 ctttctggtg tctcattctg ctttaattcg ccgtatgccc ggatcagttc actggtttca
      901 aactcccgtc gtccgtccct gccggtacgg taactgacaa ggcctgcgcg catatcacgg
      961 taaaactgtg aacgcgatct gcctgtcatt tttagtgcgt cccggagtgt gtgccatgcc
     1021 ataaagtgac agtgtcccat agatgtctca tctcatagtt tcagtaaaac ataatgaggt
     1081 ctgagaacgg taatgtttgt gctggttttt gtggcatcgg gcgagaatag cgcgtggtgt
     1141 gaaagactgt ttttttgatc gttttcacaa aaatggaagt ccacagtctt gacagggaaa
     1201 atgcagcggc gtagctttta tgctgtatat aaaaccagtg gttatatgta cagtatttat
     1261 ttttaactta ttgttttaaa agtcaaagag gattttataa tggaaaccgc ggtagcgtac
     1321 tataaagatg gtgttcctta tgatgataag ggacaggtaa ttattactct tttgaatggt
     1381 actcctgacg ggagtggctc tggcggcgga ggtggaaaag gaggcagtaa aagtgaaagt
     1441 tctgcagcta ttcatgcaac tgctaaatgg tctactgctc aattaaagaa aacacaggca
     1501 gagcaggctg cccgggcaaa agctgcagcg gaagcacagg cgaaagcaaa ggcaaacagg
     1561 gatgcgctga ctcagcgcct gaaggatatc gtgaatgagg ctcttcgtca caatgcctca
     1621 cgtacgcctt cagcaacaga gcttgctcat gctaataatg cagctatgca ggcggaagca
     1681 gagcgtttgc gccttgcgaa agcagaagaa aaagcccgta aagaagcgga agcagcagaa
     1741 aaggcttttc aggaagcaga acaacgacgt aaagagattg aacgggagaa ggctgaaaca
     1801 gaacgccagt tgaaactggc tgaagctgaa gagaaacgac tggctgcatt gagtgaagaa
     1861 gctaaagctg ttgagatcgc ccaaaaaaaa ctttctgctg cacaatctga agtggtgaaa
     1921 atggatggag agattaagac tctcaattct cgtttaagct ccagtatcca tgcccgtgat
     1981 gcagaaatga aaacgctcgc tggaaaacga aatgaactgg ctcaggcatc cgctaaatat
     2041 aaagaactgg atgagctggt caaaaaacta tcaccaagag ccaatgatcc gcttcagaac
     2101 cgtccttttt ttgaagcaac cagacgacgg gttggggccg gtaagattag agaagaaaaa
     2161 caaaaacagg taacagcatc agaaacacgt attaaccgga taaatgctga tataactcag
     2221 atccagaagg ctatttctca ggtcagtaat aatcgtaatg ccggtatcgc tcgtgttcat
     2281 gaagctgaag aaaatttgaa aaaagcacag aataatctcc ttaattcaca gattaaggat
     2341 gctgttgatg caacagttag cttttatcaa acgctgactg aaaaatatgg tgaaaaatat
     2401 tcgaaaatgg cacaggaact tgctgataag tctaaaggta agaaaatcgg caatgtgaat
     2461 gaagctctcg ctgcttttga aaaatacaag gatgttttaa ataagaaatt cagcaaagcc
     2521 gatcgtgatg ctatttttaa tgcgttggca tcggtgaagt atgatgactg ggctaaacat
     2581 ttagatcagt ttgccaagta cttgaagatt acggggcatg tttcttttgg atatgatgtg
     2641 gtatctgata tcctaaaaat taaggataca ggtgactgga agccactatt tcttacatta
     2701 gagaagaaag ctgcagatgc aggggtgagt tatgttgttg ctttactttt tagcttgctt
     2761 gctggaacta cattaggtat ttggggtatt gctattgtta caggaattct atgctcctat
     2821 attgataaga ataaacttaa tactataaat gaggtgttag ggatttaatt attctttatt
     2881 gatataaaaa gtcctagcaa tccaaatggg attgctagga ccaaacaaag tagattatat
     2941 agcataaata ggtttaattt tgctacgggg gcgttattta ggttttttct tctttcgaaa
     3001 aaatctttct ttatgaagtt aaaagctatg tattcaatag catattttga atatggacat
     3061 agaatagtgc ttatcactat tgcatatagc atcttatctg acacaaggaa ataataccct
     3121 tcgctgtttt ttgttataag gtatatatat ataagtgtgc agtacaggcc aaataaaata
     3181 ttttttatgt agtatcttaa gctcataaat taaacctcgc catatattct tttcatttta
     3241 taaggatcga gttatgagga aaagattttt tgtgggaata ttcgcgataa acctccttgt
     3301 tggatgtcag gctaactata tacgtgatgt tcagggaggg accatcgcac catcctcctc
     3361 ttctaaactg acggggatcg cggttcagta gaaaagatca aaggatcttc ttgagatcct
     3421 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc aacggtggtt
     3481 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3541 cagataccaa atactgtcct tctagtgtag ccgtagtcgg gccactactt caagaactct
     3601 gtagcaccgt ttgtgccatc atcgctctgc taatccggtt accagtggct gctgccagtg
     3661 gcgttaaggc gtgccttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3781 aactgagata ccaacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3841 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3901 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3961 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgcctgc tacgtggcct
     4021 tcttcctgtt cctggtcttt tgctcacatg ttctttccgg ccttatcccc tgattctgtg
     4081 gataactgtg ttaccgtttt tgtgtgagtc agtaccgctc gccgcagtcg aacgaccgag
     4141 cgtagcgagt cagtgagcga ggaagcggaa aagcgcctgg acgtgcattt tctccttacg
     4201 catctgtgcg gcatttcaca cccggcatgg cgtacttttc atacaatccg cactgatgcc
     4261 gcatggttaa gccagtatac actccgctat cgctacgtga ctgggtcagg gctgcgcccc
     4321 gacacccgct aaaacctgct gacgcgccct gacgggcttg tcagctcccg gcatccgctc
     4381 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatccc
     4441 cgaaacgtgc gaggcagctg cggtaaagct catcggcgtg gtcgtgaagc gattcacaaa
     4501 tatcggcctg ttcatctgcg tccagttcgt tgagcttctc cagcagcgtt aatgtctggc
     4561 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttagtcact gatgcctccg
     4621 tgtaaggggg atttctgttc atggggtaat gataccgatg aaacgcgaga ggatgctcac
     4681 aatacgggtt actgatgatg aacatgcccg gttacttgaa cgctgtgagg gtaaacaact
     4741 ggcggtatgg atgcggcggg tctgcctggg ggagccggtt gcccgttccg gaaaactgcc
     4801 gacactggca ccgccgttac tgcgtcagct ggccgccatc ggaaataacc tgaatcagac
     4861 agcccgtaag gtgaacagcg ggcagtggtc ttccggtgac cgggttcagg tggtggccgc
     4921 actgatggcc atcggggatg agctgcgccg gctgcgtctg gctgtcaggg aacagggggc
     4981 gcgggatgat agttaaattt catgccaggg gaaaaggtgg tggcagtggt ccggttgatt
     5041 acctgctggg gagggagcgt aaccgcgaag gcgcaacggt gcttcagggt aatccggaag
     5101 aagtccggga actcatcgat gccacgccat ttgcgaagaa atacacgtcc ggtgttctgt
     5161 cgttcgcgga gaaggagctg ccgccgggag gacgtgaaaa agtgatggcg agctttgagc
     5221 gtgtactgat gcccggtctc gaaaaaaatc agtacagcat cctgtgggtg gagcaccagg
     5281 acaagggacg gcttgagctg aattttgtca ttccgaacat ggagctacag accggaaaac
     5341 gcctccagcc gtactacgac cgcgcagaca ggcctagaat tgatgcctgg cagacgctgg
     5401 tgaatcacca ttacgggctg catgacccga acgccccgga gaaccgcagg acgctgacac
     5461 tccctgataa cctgcctgaa acgaaacagg cgcttgctga gggcgtcacg cgaggtatag
     5521 atgcacttta ccatgccgga gagataaaag gccgtcagga tgtgattcag gcgctcactg
     5581 aggcggggct ggaagtggtc agggtgacgc gaagcagtat cagcattgca gatccgaacg
     5641 gcgggaagaa tatcaggctg aaaggagcat tttatgagca atcttttgca gacgggcgcg
     5701 gagttcgaga aaaagctgaa agagagagcc gaatctacag agaaaatgct gaacaacgag
     5761 ttcaggaggc tcggcgaatc tgtaagcgag gctgtgacat caaacgagac gaaaatcaga
     5821 gacgctatag ccctgttcac agcctcgaca gaggaatcgc tggaaaaaca ccgggaaggg
     5881 gtgaaagagg cgatgatgca gcacaggagg gacgtgttaa agctggcagg gaatacgggc
     5941 atgatgttac tggggatagt ctttctcctg tttaccgcga gtggcgggac gctctggtat
     6001 cttggaggga ggatacaggc gaacctggaa gaaatcagga agcaggaaga gacattgcag
     6061 aaactgaacg cgaagacatg gggcgtggag tttgtgcagg acgggaacag gaaattcctt
     6121 gtccttccgt acgggaaatc agcggaggtg attccctttc aggggaaaga gtgggtacat
     6181 ctgaaggagt gacacagagt gacagagctg gaaacacatt tgctgaacgc cttagagcag
     6241 ctgcaacagg actatatgca gcggctgagc gaatgggaga gcgccttcgt ggaattgcag
     6301 aagatgtttt cgcttacgca acgggacaac gcgatgctga acgagcgggt catgcagttg
     6361 agtcagcagg tgcagcactt gagcgagcag acagaacgct tgagccagtt atacagcgag
     6421 aactggagat aagagaggaa cggctgatac aggagcgcga acatgtgtta tccctggaac
     6481 gggagcgtca gccggaaata caggaacgca cgctggatgg cccttcgctg ggatggtgaa
     6541 accatgaaaa atggcagctt cagtggatta agtgggggta atgtggcctg taccctctgg
     6601 ttgcataggt attcatacgg ttaaaattta tcaggcgcga tcgcggc
//
