LOCUS       NZ_CP074015             9564 bp    DNA     circular CON 24-AUG-2023
DEFINITION  Escherichia coli strain PI24 plasmid pYLPI24c, complete sequence.
ACCESSION   NZ_CP074015
VERSION     NZ_CP074015.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18877297
            Assembly: GCF_024585825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Lopez-Dorta,Y., Brilhante,M., Hernandez-Fillor,R.E., Baez,M.,
            Espinosa,I. and Perreten,V.
  TITLE     Molecular epidemiology of Extended-Spectrum Beta-Lactamase-producing
            Escherichia coli from food-producing animals in Cuba using
            whole-genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   Lopez-Dorta,Y., Brilhante,M. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2021) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8; Canu v. 2.1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/24/2023 06:36:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,780
            CDSs (total)                      :: 4,663
            Genes (coding)                    :: 4,389
            CDSs (with protein)               :: 4,389
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 73 of 274
            Pseudo Genes (incomplete)         :: 195 of 274
            Pseudo Genes (internal stop)      :: 43 of 274
            Pseudo Genes (multiple problems)  :: 36 of 274
            Pseudo Genes (short protein)      :: 3 of 274
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP074015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PI24"
                     /isolation_source="Rectal Swab"
                     /host="Pig"
                     /db_xref="taxon:562"
                     /plasmid="pYLPI24c"
                     /geo_loc_name="Cuba: San Jose de Las Lajas"
                     /collection_date="2018-05"
                     /genotype="ST710"
                     /note="Post-weaning piglet"
     gene            1..951
                     /locus_tag="KFU81_RS23680"
                     /old_locus_tag="KFU81_23535"
     CDS             1..951
                     /locus_tag="KFU81_RS23680"
                     /old_locus_tag="KFU81_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001293213.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            959..1372
                     /locus_tag="KFU81_RS23685"
                     /old_locus_tag="KFU81_23540"
     CDS             959..1372
                     /locus_tag="KFU81_RS23685"
                     /old_locus_tag="KFU81_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229901.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            complement(1608..6515)
                     /locus_tag="KFU81_RS23690"
                     /old_locus_tag="KFU81_23545"
     CDS             complement(1608..6515)
                     /locus_tag="KFU81_RS23690"
                     /old_locus_tag="KFU81_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005204747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="WP_135409434.1"
                     /translation="MNALSALLTKIEQASPTQRDKGTTFENLCVQYFLHEPKYAELYSD
                     VLSYGGWVSQYGETVGITKKKDDGIDLVAVTKTGEFHAIQCKNYNQTKIAKKDIDSFLA
                     ASDKTYFTLRYIVASTDNWTEEAKNMLRDKAVPVTALSLTDLEQSALDWSQFDFDPAYK
                     PVMKAKKQLRPHQTPALEAVKRGLATADRGKLIMACGTGKTFTSLRIAEAVAGRGKTVL
                     FLVPSLALLSQTLDEWTQDTLIDLRCFAVCSDSDVGKKNHDDNVVVGISDLKYPATTNA
                     SSLVKAFNQPDIFGSDKPPYMNVVFSTYHSVEVIHQAQKLGFPAFDFIICDEAHRTTGA
                     TFEGDDESAFVRIHDNAYIAGQKRLYMTATPRIFGDDAKETEGVTLCSMDDKSLYGDDL
                     YVITFSKAVQLGILCDYKVIVLAVEEKHVSRRIQSLLKDENNQLKVDDAAKIVGCWKAL
                     AKQGLAGADGVHPDAMKRAVAFCQVIEKDFRGKTHKVSSKLITEMFGAVVEAYQASEIE
                     LLRETAPDKAIDPSLKLQCQVKHVDGSMNATEKKSKIEWLKADTDENVCRILSNVRCLS
                     EGVDVPSLDAVLFLTPRSSQVDVVQSVGRVMRRAEGKELGYVILPVVIPAGEEPENALN
                     NNQAYRVVWQVLNALRAHDDRFDAMINKLEFNGKDTSRMEVIAVADKVVKKTKRQTKKS
                     ELAGKARKSSGIGSAVSPVPEQFDIEFSVGEIERALYAKIVKKCGNRHHWEDWANDIAK
                     IARTHIDRIHGILENPGNQVEISAFRAFADELRDDLNNSVSDDEIIEMLAQHLITKPVF
                     DALFADYNFTDHNPMSMAMQNVLNTLQEHHLEKEASTLNSFYESVKMRADGITTAEGKQ
                     QIIVQLYDKFFRNAFPRMTERLGIVYTPVEVVDFIIHSVNDVLKKEFGQTLADKGVHIL
                     DPFTGTGTFITRLLQSGLIPSDKLAYKFKNEIHANEIVLLAYYIAAINIEAVYHSITEE
                     SEYTPFEGICLTDTFEMYEKDDLVSEVLVDNSERRKRQKALDIRVIVGNPPYSAGQGSA
                     NDNNANISYPVLDKRIKDTYALNSKATSTKNLMDSYIRAIRWASDRVGEQGVIGFITNA
                     GYIDSNSTDGLRKCLIDEFSSIYVFHLQGNSRRSSGELAKKEGAGIFEIRTPVAITILV
                     KNRERKSKGELKFFKIGDYLSREEKLEFISKVSSVDGAEIKSKWESIVPDSYNDWLNQR
                     DDEFNKFIKIGDKKERENTIFESYSLGIATGRDAWVYNFSLEKLKFNLTGFIDFYNSEL
                     KRYSEYNDKNMMVKDFVTIDTSKISWNDNLFSELKRGTSINFSESNIFHVSYRPFSKQH
                     GYVSKELIARRYQMAQIFPQPNSHNLIISVTGLGGKKGFSTLMSNCIVDLNSMDAGAQC
                     FPLYLYEESSGSDLLDGKVNGDYISKHAIVDNGLKYFKDFYQTNAISKEDIFYYIYGLL
                     HSDDYRARFCDNLTKELPRIPHVKKISDFWLFSKAGRELAELHVNYETVEPYKADIDTG
                     SLSYSQLGKDDFYVEKMKFAKKGEKDTVIYNNKIRIKNIPTEAYDYVVNGKPALEWVME
                     RQGVSTHKDSGIVNDANDWAVETMNNPRYPLELFLRIITVSLETQKIVNSLPKLDI"
                     /name="type ISP restriction/modification enzyme"
     gene            complement(6628..7212)
                     /locus_tag="KFU81_RS23695"
                     /old_locus_tag="KFU81_23550"
     CDS             complement(6628..7212)
                     /locus_tag="KFU81_RS23695"
                     /old_locus_tag="KFU81_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_010891053.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(7225..8340)
                     /gene="mobA"
                     /locus_tag="KFU81_RS23700"
                     /old_locus_tag="KFU81_23555"
     CDS             complement(7225..8340)
                     /gene="mobA"
                     /locus_tag="KFU81_RS23700"
                     /old_locus_tag="KFU81_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_016946648.1"
                     /translation="MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQE
                     SHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERER
                     LAEKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            8504..8971
                     /gene="traD"
                     /locus_tag="KFU81_RS23705"
                     /old_locus_tag="KFU81_23560"
     CDS             8504..8971
                     /gene="traD"
                     /locus_tag="KFU81_RS23705"
                     /old_locus_tag="KFU81_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_016946647.1"
                     /translation="MNKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     Typing          776..951
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(7229..8340)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.07"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg
       61 gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac
      121 cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca gatgatgaac
      181 atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga
      241 actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat
      301 gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt
      361 ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa
      421 tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag
      481 gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac
      541 catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact
      601 tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc
      661 caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa
      721 atgaatggtg acaaaatacc aacaaccatt acatcagatt cctacctaca taacggacta
      781 agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa
      841 tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg gctcacgcaa
      901 aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat
      961 ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa caactgttca
     1021 ccgttacata tcaaagggaa aactgtccat atgcacagat gaaaacggtg taaaaaagat
     1081 agatacatca gagcttttac gagtttttgg tgcattcaaa gctgttcacc atgaacagat
     1141 cgacaatgta acagatgaac agcatgtaac acctaataga acaggtgaaa ccagtaaaac
     1201 aaagcaacta gaacatgaaa ttgaacacct gagacaactt gttacagctc aacagtcaca
     1261 catagacagc ctgaaacagg cgatgctgct tatcgaatca aagctgccga caacacggga
     1321 gccagtgacg cctcccgtgg ggaaaaaatc atggcaattc tggaagaaat agcgctttca
     1381 gccggcaaac cggctgaagc cggatctgcg attctgataa caaactagca acaccagaac
     1441 agcccgtttg cgggcagcaa aacccgtact tttggacgtt ccggcggttt tttgtggcga
     1501 gtggtgttcg ggcggtgcgc gcaagatcca ttatgttaaa aacgacagga ttcatgaaaa
     1561 tcatcagaat tgtgcaggat ggtttttcca tcctgcacac ctatcgatta gatatctaat
     1621 ttaggcaggc tattgacgat tttctgagtt tccaggctga cagtgataat tctcaggaat
     1681 agctctagtg ggtatctagg gttattcatt gtttcaactg cccagtcatt agcatcattc
     1741 acaataccgc tatctttatg agtagagaca ccttgccgct ccatcaccca ttcaagagct
     1801 ggcttgccat tcacaacgta gtcataggct tcagttggaa tgttttttat gcgaatttta
     1861 ttgttataga tgactgtatc tttttcacct tttttggcga acttcatctt ctcaacataa
     1921 aaatcgtcct tgcctaactg ggaatagctc agggagcctg tatcaatatc agctttgtag
     1981 ggctcaactg tttcgtagtt tacatgcaac tcagcaagtt cacgacctgc cttagaaaat
     2041 aaccaaaaat cagatatttt ttttacatgt ggaatacgag gtaactcttt agttaaattg
     2101 tcacaaaatc tcgcccggta atcatcacta tgtaataacc catagatata ataaaaaata
     2161 tcttctttac ttatggcatt agtctgataa aaatccttaa aatactttaa cccattatca
     2221 acaattgcat gtttactaat gtaatcacca ttaaccttac cgtcaagtaa atctgagcca
     2281 ctgctttcct catataaata gagagggaaa cattgagcgc cagcatccat tgaatttaaa
     2341 tccacaatgc aatttgacat taatgttgag aaaccttttt tccctcccaa acctgttaca
     2401 gaaataatta agttatggga atttggctgt ggaaatatct gtgccatttg ataccgtctt
     2461 gcaatcaact ctttcgatac ataaccatgt tgttttgaaa atggacggta tgaaacatga
     2521 aaaatatttg actcgctaaa attgatacta gttcctcttt ttaactcgga aaataaatta
     2581 tcattccaac ttatcttcga tgtatcaata gtaacgaaat ccttaaccat catattctta
     2641 tcgttatatt cagaataacg ctttaattct gaattgtaaa aatcaataaa tccagtcaaa
     2701 ttaaatttca gtttttccaa tgagaaatta taaacccaag catctcgacc ggttgcaatc
     2761 cctaatgaat aagattcaaa aatagtattt tctctttctt ttttatctcc aatcttgatg
     2821 aatttattaa attcatcatc tcgctgattc aaccaatcgt tatatgagtc aggaacaatc
     2881 gattcccact tacttttaat ttcagcacca tcaacacttg aaacttttga gataaattct
     2941 aatttctcct ctcgcgacaa atagtctcct attttaaaga acttcagttc ccctttgctt
     3001 ttcctctctc tgtttttcac taaaatagtg atagcaactg gtgttcttat ttcaaaaatc
     3061 ccagccccct cttttttcgc taactcgcca gaactccttc ttgaattacc ttgcaagtga
     3121 aatacatata ttgaactaaa ctcatcaatt aagcacttcc tgagcccatc agttgaattt
     3181 gagtcaatat agccagcatt ggttataaat ccaattacgc cttgctcacc aacgcggtca
     3241 cttgcccatc gaatagctcg aatataagaa tccatcagat tttttgttga cgttgccttg
     3301 gagtttaaag catatgtatc tttaattctt ttatctaaga cagggtaaga tatatttgcg
     3361 ttgttgtcat tcgcagagcc ctgaccagct gagtatggag gattcccgac aatgactcga
     3421 atatccagcg ctttttgacg cttacggcgt tcactgttat caaccaaaac ctcactgacc
     3481 aggtcatctt tctcatacat ttcaaaggtg tcagtcagac aaatcccttc aaatggcgtg
     3541 tactctgatt cttccgtgat gctgtgatac accgcctcaa tattaatggc ggcaatgtaa
     3601 taggctaaca agacgatttc gttggcatgg atttcatttt taaacttgta agccagttta
     3661 tctgacggga taagaccact ttgcagcagg cgggtgataa acgtaccggt tccggtgaat
     3721 gggtcaagaa tatgcacacc tttgtcggcg agtgtttgtc cgaactcctt tttcagcacg
     3781 tcattcacgc tgtgaataat aaaatcaacc acctcaacag gcgtatacac aattcccaga
     3841 cgctcagtca ttctcgggaa cgcgtttctg aaaaacttat cgtacaactg tacgataatc
     3901 tgctgtttac cttctgcggt ggtaatgccg tctgcacgca tttttaccga ctcatagaag
     3961 ctgttcagcg tgctggcttc tttttcgaga tgatgctcct gtaacgtgtt aagcacgttc
     4021 tgcatcgcca tactcatcgg gttatggtcg gtaaaattgt aatcggcaaa cagggcatca
     4081 aagaccggct tggttatcag atgctgtgcc agcatttcaa tgatttcatc gtcggaaaca
     4141 ctgttattca gatcatcacg cagttcatcg gcaaatgccc tgaaggcact gatttctact
     4201 tgattacccg gattttcgag aatgccgtga atgcggtcga tatgtgtgcg ggcaatttta
     4261 gcaatatcgt tcgcccagtc ttcccagtga tgccggttac cgcatttttt aacgattttt
     4321 gcgtacaggg cgcgctcaat ctcaccaacg gaaaactcaa tatcaaactg ctcaggaaca
     4381 ggactcacgg ctgaaccaat gccactgctt ttacgcgctt ttcctgccag ttcagatttt
     4441 ttcgtctgcc gtttagtttt cttcaccact ttatcggcta ccgcgatcac ttccattctg
     4501 ctggtatctt taccgttaaa ctccagcttg ttgatcatgg catcaaagcg gtcatcatgc
     4561 gccctgagtg cattcagcac ctgccagaca acacggtagg cctgattgtt gtttaaggcg
     4621 ttttccggct cttctccggc gggaatgaca accggcagaa tgacatagcc cagctcttta
     4681 ccttcggcac gccgcataac acgcccgaca gactgcacca catccacctg agagcttctc
     4741 ggcgttaaaa acaacaccgc atccagagaa ggcacatcca cgccttcaga taaacaacgg
     4801 acgttagaca atatccggca gacattttca tccgtgtccg ctttcagcca ttcaattttg
     4861 gattttttct cagtggcatt catgctgccg tcaacgtgct tcacctgaca ctgaagtttt
     4921 aatgatgggt caatcgcctt atccggtgcg gtttccctga gaagttcgat ctcggatgcc
     4981 tgataagcct caaccactgc tccgaacatt tccgtgatca gtttagaact gaccttatgc
     5041 gtctttccgc gaaagtcttt ttctatcacc tgacagaacg caacagcccg tttcatcgca
     5101 tcagggtgaa cgccatctgc ccccgccagt ccctgctttg ccagtgcttt ccagcaaccc
     5161 acaatctttg ccgcatcatc gactttgagc tgattatttt catccttcag cagagactga
     5221 atgcggcggc tgacatgctt ttcttccacg gcaagcacaa taactttgta atcacacaaa
     5281 atgcccaact ggacggcttt agagaaggta atgacataca aatcatcccc ataaagactt
     5341 ttgtcatcca ttgagcatag ggtaactccc tcagtctctt tcgcatcatc tccaaaaatg
     5401 cgcggagtcg cggtcatgta caggcgtttt tgaccggcga tataggcgtt gtcgtgaata
     5461 cgaacaaagg cggactcatc atccccttca aaggtcgcac ctgtggtccg gtgagcttca
     5521 tcacaaatga tgaaatcaaa tgcaggaaaa cccagttttt gcgcctgatg gatgacctca
     5581 acagagtggt aggtagaaaa caccacgttc atataaggcg gcttatcact gccgaaaata
     5641 tccggctgat taaacgcttt gacgaggctg ctagcattcg tggtcgccgg atatttcagg
     5701 tcgctgatac cgacaacaac attatcatca tggtttttct tacccacatc actgtcagag
     5761 cagaccgcaa aacatctcag gtctatcagc gtatcctgcg tccattcatc cagcgtctga
     5821 ctcaacagtg ccagacttgg cacaaggaac agtacggttt tacctcttcc ggcaacggct
     5881 tcagcaatac gcagagaggt gaaggttttt cctgtgccgc acgccataat gagcttgccc
     5941 cgatcggcag tcgccagccc tcttttaacg gcttcaagcg cgggagtctg atgaggccgg
     6001 agttgctttt tggctttcat caccggcttg taggccggat caaaatcgaa ttgtgaccag
     6061 tccagtgcgc tttgctcaag gtctgtcagg cttaacgcag tgacaggaac ggccttgtcc
     6121 cgcagcatat ttttagcttc ctccgtccag ttatccgtag aggcaacgat atagcggagc
     6181 gtaaaatacg ttttatcgga ggcagccaga aaactatcaa tatccttttt ggcgatttta
     6241 gtctgattgt agtttttgca ctggatagca tgaaactccc ccgttttggt tacggcgacc
     6301 aaatcaatgc catcatcttt tttcttcgtg atcccaacgg tttcaccgta ctgagaaacc
     6361 catccgccat agctcagcac gtcactgtac agctcggcat atttaggttc atggaggaaa
     6421 tactgcacac aaaggttttc aaaggttgta cctttgtccc gctgtgttgg tgaagcctgt
     6481 tcaatttttg tcagcaatgc agaaagtgca ttcatcgttg atatccttaa tgtcctttat
     6541 ttgctggggt tatcagatcc ccccgacacg tttaattaat gctttctccg ccggagatcg
     6601 acgcacagcg ttctgtgctc tatgatgtta tttcttaata atcatccagg tattctcttt
     6661 atcaccatac gtagtgcgag tgtccacctt aacgcagggc tttccgtcac agcgcgatat
     6721 gtcagccagc ggggctttct tttgccagac cgcttccatc ctctgcattt cagcaatctg
     6781 gctatacccg tcattcataa accacgtaaa tgccgtcacg caggaagcca ggacgaagaa
     6841 tatcgtcagt acaagataaa tcgcggattt ccacgtatag cgtgacatct cacgacgcat
     6901 ttcatggatc atcgctttcg ccgtatcggc agcctgattc agcgcttctg tcgccggttt
     6961 ctgctgtgct aatccggctt gtttcagttc tttctcaacc tgagtgagcg cggaactcac
     7021 cgatttcctg acggtgtcag tcatattacc ggacgcgctg tccagctcac gaatgaccct
     7081 gctcagcgtt tcactttgct gctgtaattg tgatgaggcg gcctgaaact gttctgtcag
     7141 agaagtaaca cgcttttcca gcgcctgatg atgcccgata agggcggcaa tttgtttaat
     7201 ttcgtcgctc atacaaaatc ctgcctatcg tgagaatgac cagcctttat ccggcttctg
     7261 tcgtatctgt tcggcgagtc gctgtcgttc tttctcctgc tgacgctgtt tttccgccag
     7321 acgttcgcgc tctctctgcc tttccatctc ctgatgtatc ccctggaact ccgccatcgc
     7381 atcgttaaca agggactgaa gatcgatttc ttcctgtata tccttcatgg catcactgac
     7441 cagtgcgttc agcttgtcag gctctttttc aaaatcaaac gttctgccgg aatgggattc
     7501 ctgctcaggc tctgacttca gctcctgttt tagcgtcaga gtatccctct cgctgagggc
     7561 ttcccgtaac gaggtagtca cgtcaattac gctgtcacgt tcatcacggg actgctgcac
     7621 ctgcctttca gcctccctgc gctcaagaat ggcctgtagc tgctcagtat cgaatcgctg
     7681 aacctgaccc gcgcccagat gccgctcagg ctcacggtca atgccctgcg ccttcaggga
     7741 acgggaatca acccggtcag cgtgctgata ccgttcaagg tgcttattct ggaggtcagc
     7801 ccagcgttcc ctctgggcaa caaggtattc tttgcgttcg gtcggtgttt ccccgaaacg
     7861 tgcctttttt gcgccaccgc gctccggctc tttggtgtta gcccgtttaa aatactgctc
     7921 agggtcacgg tgaataccgt cattaatgcg ttcagagaac atgatatggg cgtggggctg
     7981 ctcgccaccg gctatcgctg ctttcggatt atggatagcg aactgatagg catggcggtc
     8041 gccaatttcc tgttggacaa aatcgcggac aagctcaaga cgttgttcgg gttttaactc
     8101 acgcggcagg gcaatctcga tttcacggta ggtacagccg ttggcacgtt cagacgtgtc
     8161 agcggctttc cagaactcgg acggtttatg cgctgcccac gccggcatat tgccggactc
     8221 cttgtgctca aggtcggagt ctttttcacg ggcatacttt ccctcacgcg caatataatc
     8281 ggcatgagga gaggcactgc cttttccgcc ggtttttacg ctgagatgat aggatgccat
     8341 cgtgttttat cccgctgaag gcgcgcaccg tttctgaacg aagtgaagaa acgtctaagt
     8401 gcgccctgat aaataaaaga gttatcaggg attgtagtgg gatttgacct cctctgccat
     8461 cactgagcat aatcattccg ttagcattca ggaggtaaac agcatgaata aaagcgaaaa
     8521 acaggaacaa tgggcagcag aaagagtgca gtatattcgc ggcttaaagt cgccgaatga
     8581 gcaacagaaa cttatgctga tactgacgga taaagcagat aaaacagcac aggatatcaa
     8641 aacgctgtcc ctgctgatga aggctgaaca ggcagcagag aaagcgcagg aagccagagc
     8701 gaaagtcatg aacctgatac aggcagaaaa gcgagccgaa gccagagccg cccgtaaagc
     8761 ccgtgaccat gctctgtacc agtctgccgg attgcttatc ctggcgggtc tggttgacag
     8821 taagacgggt aagcctgttg atgataccgc tgccttactg ggtgcattag ccagtctgaa
     8881 tgacctgtca cgggataatc cgaagtggtc agactggaaa atcagagggc aggaactgct
     8941 gaacagcaaa aagtcagata gcaccacata gcagacccgc cataaaacgc cctgagaagc
     9001 ccgtgacggg cttttcttgt attatgggta gtttccttgc atgaatccat aaaaggcgcc
     9061 tgtagtgcca tttaccccca ttcactgcca gagccgtgag cgcagcgaac tgaatgtcac
     9121 gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa aaggaatatt cagcgatttg
     9181 cccgagcttg cgagggtgct acttaagcct ttagggtttt aaggtctgtt ttgtagagga
     9241 gcaaacagcg tttgcgacat ccttttgtaa tactgcggaa ctgactaaag tagtgagtta
     9301 tacacagggc tgggatctat tctttttatc tttttttatt ctttctttat tctataaatt
     9361 ataaccactt gaatataaac aaaaaaaaca cacaaaggtc tagcggaatt tacagagggt
     9421 ctagcagaat ttacaagttt tccagcaaag gtctagcaga atttacagat acccacaact
     9481 caaaggaaaa ggactagtaa ttatcattga ctagcccatc tcaattggta tagtgattaa
     9541 aatcacctag accaattgag atgt
//
