LOCUS       NZ_CP073991             7538 bp    DNA     circular CON 24-AUG-2023
DEFINITION  Escherichia coli strain MB10 plasmid pYLMB10c, complete sequence.
ACCESSION   NZ_CP073991
VERSION     NZ_CP073991.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18877303
            Assembly: GCF_024585705.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Lopez-Dorta,Y., Brilhante,M., Hernandez-Fillor,R.E., Baez,M.,
            Espinosa,I. and Perreten,V.
  TITLE     Molecular epidemiology of Extended-Spectrum Beta-Lactamase-producing
            Escherichia coli from food-producing animals in Cuba using
            whole-genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Lopez-Dorta,Y., Brilhante,M. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2021) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/24/2023 06:55:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,648
            CDSs (total)                      :: 4,530
            Genes (coding)                    :: 4,307
            CDSs (with protein)               :: 4,307
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 72 of 223
            Pseudo Genes (incomplete)         :: 145 of 223
            Pseudo Genes (internal stop)      :: 43 of 223
            Pseudo Genes (multiple problems)  :: 38 of 223
            Pseudo Genes (short protein)      :: 3 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073991.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MB10"
                     /isolation_source="Cloacal sample"
                     /host="Poultry"
                     /db_xref="taxon:562"
                     /plasmid="pYLMB10c"
                     /geo_loc_name="Cuba: Santa Cruz del Norte"
                     /collection_date="2015"
                     /genotype="ST1716"
                     /note="Broiler"
     gene            complement(join(6655..7538,1..370))
                     /locus_tag="KFU65_RS23040"
     CDS             complement(join(6655..7538,1..370))
                     /locus_tag="KFU65_RS23040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_257204748.1"
                     /translation="MPGLDKDQYSILWVEHADKGRLELNFLIPNTELLTGRRLQPYYDR
                     ADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSGLPKTTQEAAEAITRGLLSLASSG
                     ELKSRQDVTDTLEGAGFKVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNASEGLRAAI
                     ESAAEEYWRDAESRILRARAVCQSGTERKREENQRRHPRPRAEYERGDGEKPAERTADC
                     RADMAHHPDGVSAADRHEREHTVVAGAADNRQLHASSSAGRYPGENDRPDVGSALSGEQ
                     RRPEVSYPTAGDENRSHPLRRDDMDPPETGVAEDDRAGKAVAERIRAATAGLLAKAGRM
                     GERLRGMAEHVWAYATGERGAERARVGLEHAGAEFKRAAAPLVRELNAIELRQRENEYQ
                     KTLELERQPQKTYRGPTL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(603..926)
                     /locus_tag="KFU65_RS23045"
                     /old_locus_tag="KFU65_22830"
     CDS             complement(603..926)
                     /locus_tag="KFU65_RS23045"
                     /old_locus_tag="KFU65_22830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_170915627.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNTGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1564..1695
                     /locus_tag="KFU65_RS23050"
     CDS             1564..1695
                     /locus_tag="KFU65_RS23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_161992909.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPKRHDLTPLAAATGRGCTRDY"
                     /name="replication initiation protein"
     gene            complement(1997..2694)
                     /locus_tag="KFU65_RS23055"
                     /old_locus_tag="KFU65_22835"
     CDS             complement(join(1997..2445,2445..2694))
                     /locus_tag="KFU65_RS23055"
                     /old_locus_tag="KFU65_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_225905514.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCCKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            3118..4383
                     /locus_tag="KFU65_RS23060"
                     /old_locus_tag="KFU65_22840"
     CDS             3118..4383
                     /locus_tag="KFU65_RS23060"
                     /old_locus_tag="KFU65_22840"
                     /inference="COORDINATES: protein motif:HMM:NF013080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="WP_157914643.1"
                     /translation="MPNEIINIILDTNPDEINSNFFFSTKEIIGLLIIACNFLFILIGK
                     RDSKFQLRHFYIFIILFVSLLIAFKPLGLALKEINKGLSELKKNAQIIKDKKNFKWDSK
                     STINDKQTVVLFLGETNRGDYLHFNGYPKNTTPNLDKENLVSFKNTISQGAYTLVSTPM
                     ILSRKGVYDNGLFNETSIISAYKEAGYETWYVSYLSKSHIGDNEINLIANEADHYVVSD
                     VNVNTLEKILNNKSKKKLIVYKTIGSHYLYHTRYPSNYDVFKPSFNEKTYSTPSLKDKD
                     KLENSYANSELFSVDKQISDFINVLKNTDGLAYLSFISDHGTAIYDDGKSLYGGNTKGN
                     YNIAHFFWFNDLYHKQHPELIQKLSINKDKKITSECFVDTSLELSFIESKIKKGCSLLN
                     DKFIEKQRLVKNGKVYDFDQDL"
                     /name="phosphoethanolamine transferase"
     gene            complement(4655..>4720)
                     /locus_tag="KFU65_RS23065"
                     /pseudo=""
     CDS             complement(4655..>4720)
                     /locus_tag="KFU65_RS23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            5106..5669
                     /locus_tag="KFU65_RS23070"
                     /old_locus_tag="KFU65_22845"
     CDS             5106..5669
                     /locus_tag="KFU65_RS23070"
                     /old_locus_tag="KFU65_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602744.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="WP_072666782.1"
                     /translation="MPKNVFFTLRNRKSHELKKTLLSIALLTASITALSGCQSNADQYA
                     ADVYDTGQLNAKQETKTVNIISVLPAKVAVDNTANKQAAQTFGAILGAVVGGVAGHNVG
                     SGSGLCTTAGAVGGGAVGAAVGTLVKDKSIVEGVSLTYKEGTKVFTSTQVGKPCQFTTG
                     LAVVISTKNNETRIQPNTTCPVKK"
                     /name="glycine zipper 2TM domain-containing protein"
     gene            5680..6216
                     /locus_tag="KFU65_RS23075"
                     /old_locus_tag="KFU65_22850"
                     /pseudo=""
     CDS             5680..6216
                     /locus_tag="KFU65_RS23075"
                     /old_locus_tag="KFU65_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /name="DUF5384 family protein"
     Typing          complement(1028..1214)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.263"
                     /coverage="86.0"
                     /evalue="9.75e-47"
     Typing          1160..1672
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.845"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..613)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.569"
                     /coverage="99.0"
                     /evalue="1.1200000000000002e-123"
     Typing          complement(610..926)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="6.609999999999999e-60"
     Typing          complement(6659..7536)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.198"
                     /coverage="99.0"
                     /evalue="4.71e-172"
ORIGIN
        1 ccaccttaaa acctgcacct tcaagagtgt cagtgacatc ctgacggctt ttcagctccc
       61 cggacgaggc taagctcagt agtccccggg taatggcttc tgcggcctcc tgcgtcgttt
      121 tcggcagccc ggacggcgtg accagcgccc ggcggttttc cggcgcgttc gggtcgtgca
      181 gccccagtct gccgttcacg atagtctgcc aggcatcgat gcgcggccgg tcggcgcggt
      241 cgtagtaagg ctggagccgc ctgccggtca gcagctcggt attggggatc agaaagttca
      301 gctccagacg ccccttgtcc gcgtgctcca cccacagaat gctgtactga tctttatcga
      361 gtccgggcat cagaacccgc tcgaagctgg ccatcagttt ttcccgctgg ccgggaggga
      421 gatcggcctc cgcaaacgac aggacgccgg aggtgtattt cttaacgtaa ggcgacgcgt
      481 caatcagctc ccggacttct tccggcttcc cctgcaggac ggtggcgcct tcacgctgcc
      541 ggtctttacc cagcagatag tccaccggcc cggcgccacc gccgcgaccg cgtggatgaa
      601 atttaacgat catcgtccgt tcctttctca agaacggcgt gccgcagccg ctcaagtccg
      661 gcatcgatgg ccatcagcgc cgccacaatc tgtaccctgt catggccagt accgccgccg
      721 gtgttaacac ggcgggctat ctggttcagg ttattgccca tgccggcaag ctggcgcagc
      781 agcgccggcg aaattgaggg gagctttccg gtgcgcgcag gcttttcgtc caggcaggtc
      841 tgccgcatcc aggccgccag cggcctgccg tcacagcgct caagcagccg ccggtgctcg
      901 tcctccgtca cccacatggt gagcatcttg ttccgtttct ctgacaaacc ggcctcctga
      961 taacgccggg acgggcggga aagcctttcc cggtcggcgc ggggttggac aagccgcagg
     1021 cgcgtcaggc ttttggcggg tttcggggcg cagccctgaa ccagtcacgt agcgctagcg
     1081 gagtgtatac tggctcaata tgttacggcg gagtaacact taataaaagt gcttcatgtg
     1141 gcaggggaaa aatgcagctg cagggcgtac aggagaaaat ggaggttaca gtgatatgac
     1201 gcctcctcgc tcactcggtc gcttccgctc ctgacgttcg gctgcggcgg gcgttacctg
     1261 caaacggata agagcaaagg catacggcat ggcaagcagg acaggaagag gatacggagc
     1321 tgccgttttt ccataggctc cgcccccctg acgagcatca cgaaatctga cgctcaaatc
     1381 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct gatggctccc
     1441 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1501 gtttatctca ttccacgcct gacactcagc tccgggtagg cagttcgctc caagctggac
     1561 tgtgtgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1621 gagtccaacc cgaaaagaca cgacttaacg ccactggcag cagccactgg tagagggtgt
     1681 acaagagatt actgatgcag agttcttgaa gttagagcca aactacggct aaactggaag
     1741 gacgaatttg gtggcagcgg tcttgtacac cctgttacca cggttcagaa gttccccaac
     1801 ttactgaacc ttcgaaaaac cacctcccca ggtggttttt tcgtttctgg ataagagact
     1861 acgacgacag acggacgatc tcaagaaaat cctctctaac aggttatatt cagagtaaaa
     1921 aaagtgagct taaatatggc tttttcaata gcagaagtgc gcgaggggcg tatcggttct
     1981 tggtaatgac tccaacttac tgatagtgtt ttatgttcag ataatgcccg atgaccttgt
     2041 catgcagctc caccgatttt gagaacgaca gtgacttccg tcccagcctt gccagatgtt
     2101 gtctcagatt cagattatgt cgctcaatgc gctgagtgta acgcttgctg ataacgtgca
     2161 gctttccctt caggcgtgat tcatacagcg gccaaccatc cgtcatccat accacgacct
     2221 caaaggccga cagcaggctc agaagacgct ccagtgtggc cagagtgcgt tcaccgaaga
     2281 cgtgcgccac aaccgtcctc cgtatcctgt catacgcgta aaacagccag cgctgacgtg
     2341 atttagcacc gacgtagccc caatgttcgt ccatttcagc gcagacaatc acatcactgc
     2401 ccggttgtat acgcgaggtt accgactgcg gcctgagttt tttaagtgac gtaaaaccgt
     2461 gttgaggcca acgcccataa tgcgtgcact ggcgcgacat ccgacgccat tcatggccat
     2521 atcaatgatt ttctggtgcg taccgggctg agaggcggtg taagtgaact gtagttgcca
     2581 tgttttacag caatgagagc agagatagcg ctgatgtccg gcagtgcttt tgccgttacg
     2641 caccacgcct tcagtagcgg agcaggaagg acatctgatg gaaatggaag ccatgcaagc
     2701 acctaaaaat caccatcata cactaaatca gtaagttggc agcattaccg agagcggctt
     2761 tttaattaaa taatgatttc ctatgatttt ttttttaaac ttacttactt acttacttac
     2821 ttatatctat gttatcaaat aatgaacatc atcaataaaa tttcaaaaaa ccatcaaaaa
     2881 aacatataca tagctttatg tattattaat ctaattttta taattaatac ttcccataga
     2941 ataatcgcta gtaatattta ttactcctta acgcttatca tacttggtgt tatttttttc
     3001 tatggttaca cttcaaaaat taattcaatt atttctttat caatatcaag ttttattatt
     3061 tcacttctat ttgttgattt aatgacatat cgaatgacct cctggtattt tcatcaggtg
     3121 ccaaatgaaa tcattaatat tattcttgat actaacccag atgagattaa tagtaatttt
     3181 ttcttttcaa caaaagaaat catagggcta ctaatcatag catgtaactt cttatttatt
     3241 cttattggaa aaagagactc taaatttcag ttaaggcatt tttatatttt cattattcta
     3301 tttgtttctt tgttaatcgc ctttaagcca ttaggattag ctttgaaaga aattaataaa
     3361 ggtttatctg aacttaagaa aaacgcacaa attataaaag ataaaaaaaa cttcaagtgg
     3421 gattctaaat ctacaataaa tgataagcaa acagtggttc tttttttagg cgaaactaat
     3481 agaggagatt atttacattt taatggttac cctaaaaaca caaccccaaa tttagataaa
     3541 gaaaatttag tttcttttaa aaacacaata tctcaaggag catatacttt agtttccaca
     3601 ccaatgatcc tttctagaaa aggtgtgtat gataatggtc tttttaatga aacatcaata
     3661 attagtgctt ataaagaggc tggatatgaa acttggtatg tttcttatct gagtaaaagt
     3721 cacataggag ataatgaaat aaatttaatt gcaaatgaag ctgatcatta tgttgtatct
     3781 gatgtcaacg tgaatacatt agagaaaata ttaaataata aaagcaagaa aaagctcata
     3841 gtttataaaa ctatcgggag tcattatctg tatcatacaa ggtatccatc aaattatgat
     3901 gttttcaaac catcttttaa tgaaaaaaca tattcaactc catctttaaa agataaggat
     3961 aaattagaaa atagctatgc taattcagaa ttattttctg ttgataaaca gataagcgat
     4021 ttcattaatg tgttaaaaaa tacagatgga cttgcatatt tatcttttat ttcagaccat
     4081 ggaactgcta tttatgatga tggtaaatcg ttgtatggcg gtaatacaaa aggcaactat
     4141 aacattgctc attttttttg gtttaatgat ttataccaca aacaacatcc tgagctaata
     4201 caaaaacttt caattaataa agataaaaaa ataacatccg aatgtttcgt agatactagt
     4261 ttagagttat cctttattga aagtaagatt aaaaaaggtt gttccctact aaacgataaa
     4321 tttattgaaa aacagcgatt ggtgaaaaat ggtaaagttt atgatttcga ccaagattta
     4381 taaaatgatt tccatataat tgaactagct aaaacttaac ctggcagtta cttattttta
     4441 ttaggtgtct gtcaggttaa atatgtttac taagttatac aaaatataag acactgttgc
     4501 aaagttaggg aaggtgcgaa taagcgggga aattcttctc ggctaactca gtcatttcat
     4561 ttcttcatgt ttgagccgat tttttctccc gtaaatgcct tgaatcagcc tatttagacc
     4621 gtttcttcgc catttaaggc gttatcccca gtttttagtg agatctctcc cactgacgta
     4681 tcatttggtc cgcccgaaac aggttggcca gcgtgaataa ggggtgagtt tggagattgg
     4741 aaattattgt acgttaagcc tgttttttga acagatcgcg ctatcgcaat agggtgtact
     4801 cctgcactat acggcattta gcctgtatgt ttgggtaatg agacaaccta aatgcgacag
     4861 cgtgagcgca ataacttaaa tgcgatagtc agtaacaata aatcaatcag ttaccaatca
     4921 gaaacggtta aattatgttc ttaacgtaca ataatttcca tttcccaaac cgaccccagg
     4981 aaggacatct gatggaaatg gaagccactg gagcacctca aaaacaccat catgcactaa
     5041 atcagtaagt tggcaccatt accggaaaat caaccaccac ctttagcctt tactttttac
     5101 ggataattcc taaaaatgtt ttttttacat taagaaacag gaagtcacat gagctcaaaa
     5161 aaacactgtt aagcatagcc ctactaaccg cctcgattac cgctttatca ggctgtcaat
     5221 ccaatgccga tcaatatgct gctgacgttt atgacacagg gcaactgaat gcaaaacagg
     5281 agacgaaaac tgtaaacata atttctgttt tgcctgcgaa ggttgctgtt gataacactg
     5341 caaacaaaca agctgcacaa acctttgggg caatccttgg cgctgtggtt ggcggtgtgg
     5401 ctggtcataa cgtcggaagt ggtagcggac tttgcacaac agccggagcc gttggcggtg
     5461 gtgccgtcgg tgccgcagta ggaacattag tcaaagataa atctatcgtt gaaggtgttt
     5521 ctttaactta caaagaaggg acaaaggtat ttacatcaac tcaagtagga aaaccatgtc
     5581 agtttactac tggtttagct gttgttattt ccacaaaaaa taatgaaaca agaatccagc
     5641 caaacacaac atgcccggta aaaaaataag attcaaacga tgaaaagaat cattataccc
     5701 ctcatccttt cttttagtgg aagcagcttt gcaaactcac ttcaagaaca attatctgca
     5761 gtagctcaaa ctgaacagca gggaaaagct gaagagcaaa gactcatgga tatccagaga
     5821 gaaaaaattg aggaaaacaa acggcttgag caaaaaagga gagaaagagc aagagcagaa
     5881 gcgaacagga gagcagccat agcttctgca gagagaaaag ctcaccgtga aagaatcgaa
     5941 gctgagctca aagccgataa aaatagagat cagaaatatg aagatgaact aagaaagctt
     6001 gaaatcgaaa aacaaaagtt agcattagcc agggaagagg caagagttaa acgcgaaaat
     6061 taattcatag accaagagtt aaaaaacaag gcagcccaaa cagatgtaat tcaatctcac
     6121 gccggctcaa acagaaatat ttcagaaggc gggaaagatt taatgaaaag cgaagggaaa
     6181 gccagagaaa aagaagctgg agaatggttt aactagcttc atttttataa ttaaagttga
     6241 tttcaagttt aattaacaac ggatgttatt taccataaca tctgttgccc gcaccgctcc
     6301 ggacgggttc gccacaaacg gggcggcagt caggtaaaac cggcaacaaa ccacccgata
     6361 caccgccccg ttcgtgacag ataaatttta cccgactgca tagccatgca gccagatgca
     6421 tggcgtgtaa agtcacacta aaatcagcgc gaacgcctta aaaacaggta aatgcttctc
     6481 cagccagtcc cacagcccgg ccgccagata caggcagtta agtacagctt caatgacgtt
     6541 aagcaatttc ataattcccc cccaggtata ggccgtgatc tttttaatca cgaacctatt
     6601 tagcgggaga agtaagagcc cctcttcgtg cgggttgtca gctgcctgga tggtttaaag
     6661 tgtcggcccg cggtaggttt tctgcggctg gcgctccagc tccagtgttt tctgatactc
     6721 attctccctc tgccggagct cgatggcatt cagttcccgg acaagcggcg cagctgctcg
     6781 cttaaactca gcacctgcgt gctcaagtcc gacacgcgct cgttcagcac cgcgttctcc
     6841 cgttgcataa gcccacacat gctccgccat tccgcgaagg cgctctccca ttcgtccagc
     6901 ctttgcgagt agtcctgctg tagctgctct aatgcgctca gcaactgctt ttccagctct
     6961 gtcatcttcc gctactcctg tttcaggcgg atccatgtcg tcccgccgta agggatggct
     7021 tcggttttca tccccggcgg taggataaga aacctccggc cgtcgctgct ctcctgatag
     7081 cgcactcccc acgtccgggc ggtcattttc gccagggtat cttcctgctg acgaagatgc
     7141 gtgtaattgt cggttatctg ctgcccctgc caccacagta tgctcccgct cgtgccgatc
     7201 agcagcgctg acaccatcag gatggtgagc catgtccgcc cggcagtccg cagtacgctc
     7261 cgccggtttt tctccatcgc ctcgctcata ctctgctcgt gggcgaggat ggcgtcgctg
     7321 attctcctcg cgcttgcgct cagttccgct ctgacagact gctcgtgctc gcagaatgcg
     7381 gctttcagca tctcgccagt actcttctgc tgcgctttcg attgctgctc taagtccttc
     7441 gctagcgtta aaagattgct catagatagc tcccttcagt cggatgtttc gccccccgtc
     7501 cgggtcggca atgctgatac tgcttttcgt ggtgcgca
//
