LOCUS       NZ_CP073979            11809 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Escherichia coli strain MB20 plasmid pYLMB20c, complete sequence.
ACCESSION   NZ_CP073979
VERSION     NZ_CP073979.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18877306
            Assembly: GCF_024585645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Lopez-Dorta,Y., Brilhante,M., Hernandez-Fillor,R.E., Baez,M.,
            Espinosa,I. and Perreten,V.
  TITLE     Molecular epidemiology of Extended-Spectrum Beta-Lactamase-producing
            Escherichia coli from food-producing animals in Cuba using
            whole-genome sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11809)
  AUTHORS   Lopez-Dorta,Y., Brilhante,M. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2021) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 11:02:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,950
            CDSs (total)                      :: 4,832
            Genes (coding)                    :: 4,638
            CDSs (with protein)               :: 4,638
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 53 of 194
            Pseudo Genes (incomplete)         :: 138 of 194
            Pseudo Genes (internal stop)      :: 38 of 194
            Pseudo Genes (multiple problems)  :: 34 of 194
            Pseudo Genes (short protein)      :: 4 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073979.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MB20"
                     /isolation_source="Cloacal sample"
                     /host="Poultry"
                     /db_xref="taxon:562"
                     /plasmid="pYLMB20c"
                     /geo_loc_name="Cuba: Santa Cruz del Norte"
                     /collection_date="2015"
                     /genotype="ST10"
                     /note="Broiler"
     gene            complement(join(11658..11809,1..490))
                     /locus_tag="KFU64_RS24560"
                     /old_locus_tag="KFU64_24335"
     CDS             complement(join(11658..11809,1..490))
                     /locus_tag="KFU64_RS24560"
                     /old_locus_tag="KFU64_24335"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_257183130.1"
                     /translation="MTEPTRPILNIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQIAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(487..1776)
                     /locus_tag="KFU64_RS24565"
                     /old_locus_tag="KFU64_24340"
     CDS             complement(487..1776)
                     /locus_tag="KFU64_RS24565"
                     /old_locus_tag="KFU64_24340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_145062810.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCHEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTASVCSDCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFTQNETRSLAKIPV
                     ILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLTLSVLAAVGRWDYINDI
                     IRRIDRDRGDAATLIALADHIQTNRPERDL"
                     /name="hypothetical protein"
     gene            2132..2341
                     /locus_tag="KFU64_RS24570"
                     /old_locus_tag="KFU64_24345"
     CDS             2132..2341
                     /locus_tag="KFU64_RS24570"
                     /old_locus_tag="KFU64_24345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186834.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRAADGVR"
                     /name="hypothetical protein"
     gene            2328..3500
                     /locus_tag="KFU64_RS24575"
                     /old_locus_tag="KFU64_24350"
     CDS             2328..3500
                     /locus_tag="KFU64_RS24575"
                     /old_locus_tag="KFU64_24350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257183135.1"
                     /translation="MAFVDFDTTHLKEDPGLFWRAVSAAGAPADRDEWVRLCLALGCWA
                     SLCGEDGLAAIEAWSTRHGRGENRHRQQYRSFLKGDLDTFAAQAFLNFSGVEWRNGRAS
                     VQSDVDRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALA
                     AAGGVQLPALSTDLGVGGMFRSFNPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCS
                     AEGFQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLS
                     RGKVAVCAAFSIAGFGSCVASLRRFWPDADVMLVPDHGEAYRLAHKAAYGDEREPVPTI
                     PGAAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERR"
                     /name="hypothetical protein"
     gene            3731..4189
                     /locus_tag="KFU64_RS24580"
                     /old_locus_tag="KFU64_24355"
     CDS             3731..4189
                     /locus_tag="KFU64_RS24580"
                     /old_locus_tag="KFU64_24355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_174599770.1"
                     /translation="MKSKTELRAAATARALEVIASEMGGWSLDGFAHGSLPNFSPLPRQ
                     QTQEGSVVLERPPFDYTWAGTAAFTDRANRALQVKLPASRERNYIWLCAVEREAVATAL
                     IVESFNVTGCAAFAGLPPVDGMVLLTMDEADVELIRAAMLPWLDAAAA"
                     /name="hypothetical protein"
     gene            complement(4207..4695)
                     /locus_tag="KFU64_RS24585"
                     /old_locus_tag="KFU64_24360"
     CDS             complement(4207..4695)
                     /locus_tag="KFU64_RS24585"
                     /old_locus_tag="KFU64_24360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257183139.1"
                     /translation="MREKTRTLTVRLPVSLIERIEQRAEKTKVTRTDAVIEALVAGLGT
                     KPEGMDTKLLTAIEALRSEVRALAGGGKDARQSAPQIARQSAPQNARQIVPRGADPRFQ
                     AVSDEADQLIVSMTGENATLPAIAQALNAAGHTTATGQEWNTSRVRDYRHKLRERGLI"
                     /name="hypothetical protein"
     gene            4815..5099
                     /locus_tag="KFU64_RS24590"
                     /old_locus_tag="KFU64_24365"
     CDS             4815..5099
                     /locus_tag="KFU64_RS24590"
                     /old_locus_tag="KFU64_24365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257183141.1"
                     /translation="MASPWVPVDSFCGQVFCGGAGPWISLKRCEAVLWLAWRGITTARE
                     VRGGQWREWLGSGKLIWRLWIRLGIFGHKKRRPVGWSPFSAGGVGRSSD"
                     /name="hypothetical protein"
     gene            5268..7055
                     /locus_tag="KFU64_RS24595"
                     /old_locus_tag="KFU64_24370"
     CDS             5268..7055
                     /locus_tag="KFU64_RS24595"
                     /old_locus_tag="KFU64_24370"
                     /inference="COORDINATES: protein motif:HMM:NF017826.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="WP_061301013.1"
                     /translation="MSQIDENSGAVADNVTPIDAAKGKPAADGSDLPFIRFSGGWVYLK
                     TCKTDKKGETYYSLQAVCKSHLRVIGTGWVDGEAFRVLELGKDKRRVMMPTRLIGSRDG
                     WAFLQGHNVQVTPVPELRRWLHEYLMSETAYDLHGSPCGVLPEWDVADKPGWRGGAYLL
                     PDGDMIGDVGNVIADFKLRSNHADVGVCGTVDSWVENVGRLLYGNDSPTLALGVAMASP
                     MLGVLGVSGFGVHLYGQSSGGKTSSCYPAMSIFGSPSNRQASWNSTPLALTLLARCCND
                     ALMMLDEMKLLRAMVMEEAIYGTFSQRSRMQGNKDGTLRPEYSWRCCVLSTGELAADVF
                     IRMMTGRDADAGALVRLVQLPYRPLTNLHGFPDGSAFADHLRAASDEHYGAVGRAWVDW
                     LSSNQTLAREKFAEVKARWNGRGTHGQHQRVADYFAVIETALTLSSKTLKLDVKECQGV
                     LDRFYADWLVDFSKGGNGSHEEALLVERLEAVLSQHQRFLSKTANDQDYKQPGELWGYF
                     HDRGDAREYLLFPAVFKERISGTIDAKVAGRLLHTRGLLRRHGEHLTVSERAGGYPNKR
                     RFYALSLPVVDDLDAEEVL"
                     /name="DUF927 domain-containing protein"
     gene            7055..7240
                     /locus_tag="KFU64_RS24600"
                     /old_locus_tag="KFU64_24375"
     CDS             7055..7240
                     /locus_tag="KFU64_RS24600"
                     /old_locus_tag="KFU64_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_061301012.1"
                     /translation="MAKPTPAESAMNVTRFIRAQSLIMLEKVEALDFDDLTALAEKLHD
                     DAEYLELLVAKRVRNP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8007..8474)
                     /locus_tag="KFU64_RS24605"
                     /old_locus_tag="KFU64_24380"
     CDS             complement(8007..8474)
                     /locus_tag="KFU64_RS24605"
                     /old_locus_tag="KFU64_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064186840.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRRWASEKA
                     AAPSNVLSIEEMRKRAAAAVEATPQAAIRRLKNHFVQQQFDLLQRHAKDLQALRSASLQ
                     AMLEKDATMVKAISGLMTALLKAQERERLIYEIKPGTEADIMREGMNRKQQ"
                     /name="helix-turn-helix domain-containing protein"
     gene            8692..8946
                     /locus_tag="KFU64_RS24610"
                     /old_locus_tag="KFU64_24385"
     CDS             8692..8946
                     /locus_tag="KFU64_RS24610"
                     /old_locus_tag="KFU64_24385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162734320.1"
                     /translation="MLLTPVRLSLWSCLPCVKADDADGAVSPCLVAEGSLVLASAVGGA
                     MTRLAKLADLAVFDAQARVAGRGGLLYALHVIKPLTGGW"
                     /name="hypothetical protein"
     gene            complement(9027..9614)
                     /locus_tag="KFU64_RS24615"
                     /old_locus_tag="KFU64_24390"
     CDS             complement(9027..9614)
                     /locus_tag="KFU64_RS24615"
                     /old_locus_tag="KFU64_24390"
                     /inference="COORDINATES: protein motif:HMM:NF012464.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_064186842.1"
                     /translation="MNKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQLLQEGDTLVIYAIDRIGRNARDTLELVEELEKRKITLVSIREGVDLS
                     SAVGQAVLGVMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            9874..10533
                     /locus_tag="KFU64_RS24620"
                     /old_locus_tag="KFU64_24395"
     CDS             9874..10533
                     /locus_tag="KFU64_RS24620"
                     /old_locus_tag="KFU64_24395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_112918731.1"
                     /translation="MTEIKTECPDAAWLRTTLAEMAGDAFPVYDLPSLRVDPNSSTQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAEADGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            10533..11105
                     /locus_tag="KFU64_RS24625"
                     /old_locus_tag="KFU64_24400"
     CDS             10533..11105
                     /locus_tag="KFU64_RS24625"
                     /old_locus_tag="KFU64_24400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257183119.1"
                     /translation="MSAFKLLCDRLRNEATLAKSDGAGAFKKQSLDPDLKGGKKGKKPA
                     GELEEDEAIASLHRKRMNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAP
                     IGAPVASPTEAVHRARAQFIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAP
                     AFAEVRRQVQAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            11098..11460
                     /locus_tag="KFU64_RS24630"
                     /old_locus_tag="KFU64_24405"
     CDS             11098..11460
                     /locus_tag="KFU64_RS24630"
                     /old_locus_tag="KFU64_24405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186829.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPVLRLHPGDRSVT
                     ERRALGRFRGSLAAGGQTDAFVRSDASLYALGLRFQRKRTRELVASDPDVAAAGEALEQ
                     RANGVNLSETFGGGDE"
                     /name="hypothetical protein"
ORIGIN
        1 ggctctgctg gtacgagctg gtacccgtga caatgcccag gatgcgacgg acggctttgc
       61 ggtctgcctc tggcggcatg tccagctctg cgcctcgggt caggatctgc tcgatgatgc
      121 cctttgccat ggggcgacgg tcaactcccc gggtcagtaa gtcaggccag tgctctttca
      181 ggaatgcttt ggtcacccgg ttggccagcg cctttttgga cttgttggcg atcgcgatgt
      241 cctgctggcg gcggtcgttc tcctcctgtc tgcggatccc ctcggcgatc tgctcgggcg
      301 tctttttcgg ctttgccgct gtgggcttct gtgcgggctt ctgcggggct ttcgcttttg
      361 gctgtacctt cgccttgctg gtagtccgtt cggctggctg tggctcagat ttcgcggcct
      421 tttccggcgg gacgtagacc gtgcggttgc gtcgcttgat gttcaggatc gggcgggtgg
      481 gttcggtcat aaatctctct caggtctgtt ggtctggatg tggtcggcaa gggcgatcag
      541 ggtggcggcg tcgccgcggt cccgatcgat gcgccggatg atgtcgttga tgtagtccca
      601 gcgcccgacg gcggcaagga cggaaagggt gaggcggttg aaccgactct ctggtacccg
      661 gtcgagggtc agggggatgc gcttgcgggg gcgtcctgga ccgcgccctg tccctggtct
      721 tttgagcagc tgcggctgct ccctctgcgg caaaataacg gggatcttcg ccaggctgcg
      781 cgtctcgttc tgcgtgaact cgggcaccgc gatgtgctcg tctgtccatg tcctgcctcc
      841 gggcctggct gatgcgctgg agtcgcagga cgggcaggag acgacgatct cccgtcgcgg
      901 cttgcccttg accggtgcct tgctgatcac caggggcgcg gctttgcaat cggagcagac
      961 cgacgctgtc cagaccgcgt tgtctggcag ggtggcgttc cggtactggt gcgcggcgtc
     1021 atgcggtgtc tggaagctga ccgtctgaaa cgtgggggcg acgctgaggg gtgccgccca
     1081 tggctggcac ctgggacaag caagctccac ctctccggcg tgcccgtcgc caccgttcca
     1141 gcgcaggggc tcgcggtgtt cgtggcagta gtacacgaaa ccgtcgcggc gtgtttgccc
     1201 gaccgggcct ggtcggcgat cgtggaaccg tgcgcccggg ttggctcgca tggcgcggac
     1261 gagcgcctcg ccctggtgga tgccgtacca gacctgccag ccatcgccga tgccgatcag
     1321 gaacggcgag acgaacggtg gggtgcggtt gactctcccg cgtgcgttgg ctccgatggt
     1381 ggcttcgcgt tcgcggttcc agcaggagac gcacaggctc gaaccgggct tattgggggc
     1441 gatcaggcgg ttgtctttcc ggctgcaacg ggggcagagc ttacgggtgt ctgccggggt
     1501 ggtcagtggt gcgtcagggg gctgttcggt accgacccaa agcgcgaggc cagtggggca
     1561 ccggtggcac tcgaggtggg ggttttcaat cgtgcgatcg aggtggctct gctcataaat
     1621 ctgtcggcat gcctcgaccc ggagggagca cccgccgggg caggtgacga acaaaacgcc
     1681 ggggagctgc tcggggaatt tttcagtcgc gcttacgcgt tcaagtgctg ccgggttcgg
     1741 ctgcgtcgat tcgttcgtgt cgttatcgtc gttcactgct ccccctgcgt cgaaatggtc
     1801 gccagacagc gccagcactg gctgtgcgtc ttttttagta tctcacaaaa aacgccactt
     1861 tggcgttaat tgtgttacgc ttgtcttact gattcctgac gagcagttgg gaaacatgaa
     1921 accggatcgc ccggtgccgc tttccctcct ccccaacgag gcggggttgt aaacgaggat
     1981 gcgcgcttcg tccttggcag gtgcgagtaa gcgagaaggg ctgctgtcct gccgctatgc
     2041 ggtaagtcct caagggcggc tttatcgata atcgtcactc ccgccccccg atgcccgtta
     2101 agctgagggg ggcacctatc tggtactccc aatgaaaaaa cgaatctata ccgctgacct
     2161 cgctgcgctg ctgaacgtgt cgctgcgcac gctctcgcgg ctaagttcgc gggggatcgt
     2221 gccgactccg gcagggcgct ccctccgtgg cgggcattac tggctggctg gcaatcccga
     2281 gatcaaacgc ttcgttgccg accagcgtcg tccggggagg gctgctgatg gcgttcgttg
     2341 atttcgacac tacccatctg aaagaggatc cgggcctgtt ctggcgtgcc gtatcggctg
     2401 cgggtgctcc ggctgatcga gacgaatggg tgcgtctctg cctggctctt ggttgctggg
     2461 cgtctctctg cggcgaggac gggctggcag ctatcgaggc ctggtccact cgacatgggc
     2521 gaggcgagaa tcgccatcgc cagcagtaca ggtcgttttt aaaaggcgac ctggatacat
     2581 tcgccgctca ggctttcctc aatttctccg gcgtcgaatg gcgcaacggt cgcgcttctg
     2641 tccagtccga cgtcgatcgc gctgaacggc tgcggttgtc ccgtgaggcc acagcgaagg
     2701 caaaggctga acgcgacaag aagggtgatg ctgctgcgcg tgctgcggtc aagctgctgg
     2761 cgaagggagg ggatcctgcg tctaccccat actggcgcaa aaagaccgct ctggcggctg
     2821 cgggtggcgt ccagctgccc gccctcagca cagatcttgg tgttggcggg atgtttcgat
     2881 cgttcaatcc gcgtgcttac tggtctgatt gcctcctgat tccggtttac aagttcgacg
     2941 ccgcccgtca gctgtatgtt gacggcctgg aggccatctg cgggcgttgc tcggctgagg
     3001 gtttccagct cactggctcg aagttctctc cggcgggtac ccgcaagcag ggcggcttct
     3061 acccgatgcc ggggttcatg gacgtgctcg acggctcccc gcacgcgccc tactcgcctc
     3121 tgttcgtctg tgaggggttc gctacggcgt tcgccctgca ccacctgagc cgtggcaagg
     3181 tggcagtctg cgcggcgttc tcaattgccg ggttcggcag ctgcgtggcg tcgctgcgtc
     3241 ggttctggcc agatgctgat gtgatgctgg taccggatca cggcgaagcg taccgcctgg
     3301 cgcataaagc ggcctatggc gatgagcgag agccggtccc gacaattcca ggcgcggcgg
     3361 ttgtgtcgtt cgacggcctc gatcggttca ctccccggga taacctcgac tggttcgacg
     3421 tgctggttga ggaaggggaa gatcgggcac gtgagctgct ccggtatgcg gttcgtcatg
     3481 cccggcttga gcggcgttga ttgacgtgtc cgaatggtgc gcctttcggg acacacctcc
     3541 tgctgccgct gccccggatt ttgcgaccga ctttcgagac actatcgacg cttttttgca
     3601 tagacggatt aaagcgacgc accgccgctg cggtcgtgac gctatcctgc agctggtttg
     3661 gtttccagca gcacactcaa gcccggcacc gttttggtgt cgggcttttt ttcgcccgta
     3721 aggagggcac atgaaatcaa agactgaatt acgcgctgcc gcgacggctc gcgcccttga
     3781 ggtgatcgct tctgagatgg ggggttggtc gctcgacggg tttgctcacg gctcgctgcc
     3841 gaacttctcc ccgctgccgc gccagcagac gcaggagggc tctgtggtgc tcgaacgccc
     3901 gccgttcgac tacacctggg cgggtaccgc tgcgtttacc gatcgtgcaa accgtgcgct
     3961 gcaggtgaag ctaccggcgt cgcgtgagcg taactacatc tggctgtgcg cggtcgaaag
     4021 ggaggctgtg gccaccgcgc tgatagtgga gtcgttcaac gtgaccggct gcgcggcgtt
     4081 cgccgggttg cctcctgtgg atggcatggt gctgctgacc atggacgagg ccgacgtgga
     4141 gctgatcagg gcggcgatgc tgccgtggct ggatgcggcg gcggcgtgag ccgtcgcccc
     4201 tctgcgtcaa atcaggccgc gctcgcgcag cttgtggcgg tagtcccgga cgcggctggt
     4261 attccactcc tgcccggtgg cggtggtgtg cccggctgcg ttcagcgcct gggcgatcgc
     4321 gggcagggtg gcgttctccc cggtcatgct gacgatgagc tggtcggcct catcgctgac
     4381 ggcctggaag cggggatcgg ctccccgggg gactatctgt cgtgcgtttt gtggtgcgct
     4441 ttgtcgtgct atttgtggtg cagactgtcg tgcatctttc ccgcctccgg ccagcgccct
     4501 gacctctgac cgcagcgcct cgatggcggt caaaagtttg gtgtccatcc cttccggctt
     4561 cgtgccgagc ccggcgacaa gcgcctctat gacggcatct gtgcgggtta ctttggtttt
     4621 ctccgctcgc tgctcgatgc gctcgatcag gctgacgggc agtcgaacgg ttagggttct
     4681 ggttttctct cgcatgggct ggcctctccg tgtcgtgcgt tttgtcgtgc tgtttgtctt
     4741 gcggagtgta atgcgttttg tcgtgcgcgt cgaatttgac gtagtgcaat tgtcgtgcag
     4801 ggaggggcga cggcgtggcg tcgccttggg tgcctgtgga cagtttctgt ggacaagtat
     4861 tttgcggcgg ggctggcccg tggataagtc ttaaacgctg cgaggctgtg ctgtggttgg
     4921 cgtggcgcgg gatcacgaca gcgcgagaag tgcgcggcgg tcagtggcgt gagtggttgg
     4981 gatcgggtaa gctgatctgg cgtctgtgga tccggttggg gatcttcggg cacaaaaaaa
     5041 ggcgacctgt tgggtggtca cctttttctg ccgggggagt tggtcgctcc tccgattaat
     5101 tcgagtcacg tcttagcggg acgttctcga aggaagaata gcacttgcgc agcgtactcc
     5161 tcaaactcaa agagaagtgt accacgtcga taagatcgcg caagtgtgtt ttttcctcgt
     5221 aagcccccgc cagacgccga ctccttaatt ttaaaagggg taattacgtg tcacaaattg
     5281 atgaaaattc cggcgctgtt gccgacaacg taacgccgat cgatgctgcc aagggcaaac
     5341 ctgccgccga tggctctgac ctgccgttca tccggttttc cggcggctgg gtgtacctca
     5401 agacctgcaa gactgacaag aagggcgaaa cctattactc gctgcaggct gtctgtaagt
     5461 ctcacctgcg tgtcatcggc accggctggg tcgatggcga ggcgttccga gttctggagc
     5521 tgggaaagga caagcgccgg gtaatgatgc cgacgcgcct gattggctcg cgagatggct
     5581 gggcgttcct tcaggggcat aacgtgcagg tcactccagt tcccgagcta cgtcgctggc
     5641 tccatgagta cttgatgtcc gagacggcct acgatctgca tggctctccg tgcggcgttc
     5701 tacccgagtg ggacgtggct gacaagcctg gttggcgcgg tggggcgtac ctcctgccag
     5761 atggcgacat gataggcgat gttgggaacg tgatcgccga cttcaaactg cgaagcaatc
     5821 acgccgacgt tggtgtgtgc ggtaccgtcg actcctgggt ggaaaacgta gggaggctgc
     5881 tgtacggcaa cgactcgccg accctggcac tgggggtggc gatggcctca cctatgcttg
     5941 gagtgctcgg cgtgtccggg tttggcgtcc acctgtacgg gcagtcctcc ggtggtaaga
     6001 cctcctcctg ttacccggct atgtcgattt tcggatctcc ttcaaaccgt caggcttcgt
     6061 ggaacagcac gccgctggcg cttactctgc tggctcgctg ctgcaacgac gcactgatga
     6121 tgctagacga aatgaagctc ctccgggcta tggtcatgga agaggcgatc tacggcacct
     6181 tctcgcaacg atcgcgcatg caggggaaca aggacgggac gctgcggcct gagtattcct
     6241 ggcgctgctg cgtgctctca acgggtgagt tggctgctga cgtattcatt cgcatgatga
     6301 ccgggcgtga tgctgacgca ggggcacttg ttcgcctggt gcaacttccc tatcgcccgc
     6361 tgacaaacct ccacggcttc cctgacggct ccgcgttcgc cgaccaccta agggcggcat
     6421 ctgacgagca ctacggtgct gttgggcggg cgtgggtgga ttggctgtcg tcaaaccaga
     6481 ctctggcccg ggaaaaattc gcagaggtta aggctcgttg gaacgggcgg ggtactcatg
     6541 gccagcatca gcgcgtggcg gactacttcg cggtgatcga gacggcgctg acgctgtcct
     6601 ctaaaacgct caagctcgac gtgaaagagt gccagggcgt gctcgatcgc ttctacgccg
     6661 actggctcgt cgatttcagt aagggtggca atggctccca tgaagaggcg ttgctggttg
     6721 aacggctgga ggcggttctg tcgcaacatc aacggttcct gtccaagacg gcgaacgatc
     6781 aggactacaa gcagcccggc gagctgtggg gctacttcca tgaccggggc gacgcgcgcg
     6841 agtatctgct attccctgcg gtgttcaaag agcggatctc cggcaccatc gacgccaagg
     6901 tggcggggcg cttgcttcac actcgtgggc ttctgcgtcg tcatggtgaa caccttacgg
     6961 tgtcagagcg tgctggcgga tacccgaaca agcgccgctt ctacgcgctg tctctccccg
     7021 tggttgacga tctggacgct gaggaggtgc tctgatggct aaacctaccc ccgctgaaag
     7081 tgccatgaac gtgacgcgtt tcatccgtgc gcagtccctc atcatgcttg aaaaagtgga
     7141 ggcgctcgac ttcgacgacc ttaccgcgct ggctgaaaaa ctccacgacg atgccgaata
     7201 cctggagcta cttgtcgcca aacgggtaag aaatccctga tcggtagcat tggaaccagt
     7261 ccactggttc cacgcaaaaa accgctacag cccgcgtcct gcgggctttt tattgcaatt
     7321 cgtccgtaaa gttaaaaacc tactggttcc gcactggttc cagctacagg ccgcgtcatt
     7381 gctgggctac agcgttttgg aaccagtgga accagtggta ccggtgattt tgtttgtctc
     7441 taagaggatc cctcaattgg cggtaatcga aaagcctgtg cgcacatacg tgtattattt
     7501 tttactggtt ccactggttc cactggttcc aatgtcctgt aagtggcgtg gtttctgggt
     7561 ttgttttgga accagtgagt aatcatgact ggttccagac tggttccact ggttccactt
     7621 ggtgggtgta atgggtgtaa tgctcaattc ctgcgcagtt gacgcgccgc agctgcaccc
     7681 tgcgggatgc actttgcgtg tcttggtcga tcggtggatg ggttcgtcgt gccggggtgg
     7741 ttggggtggc gctgagggca cgagctgtcg ctcgcgctag gtggtcgtct gttggttctg
     7801 cgtgagggct tgccctcgcg ccctctgcgt tatgggtgga atgcgtagct gcagctgcgc
     7861 gtttggttcg tccgggcatc aaaacggggc ttctcgcgca cgcgagcggt aacaattgcg
     7921 taacgtgcgg atccgcgtat tccgtgggct cccgggtttt ggcggtcaca aaaacggtca
     7981 catggcggtc atttcggtca tggcggtcat tgttgtttgc ggttcatgcc ctcgcgcatg
     8041 atgtcggcct cggtgcccgg cttgatctcg tagatgaggc gttcgcgttc ctgcgctttg
     8101 agcagggcgg tcatgaggcc gctgatcgct ttgaccatgg tggcgtcctt ctccagcatg
     8161 gcctgtaacg atgcgctgcg gagggcttgc aagtcctttg cgtggcgctg tagcaggtca
     8221 aattgctgct gtacaaagtg gtttttaagg cgtcggatcg cggcctgtgg tgtggcctcg
     8281 acggcggcgg cggcgcgttt ccgcatctcc tcgatgctga ggacgttgct gggtgctgcg
     8341 gccttctcgc tcgcccatcg gcgaacggtg cggggtggga tccccatggt gcgtgcgacg
     8401 gtggcactgg ttgcgccgtc ctgctccagt agcctgagcg cctgggcttt ctgttcggtg
     8461 gtgtatttct tcatggttcc tgctccggtt ggtttctctc ccgtattgaa acgtcacgcg
     8521 ctgaaaatgg cgggggtttt tggggggtac acggcgtttg tgcgtgggtg gtcgtgcggt
     8581 tgggtgtttt tgtggctgag gcgcttagaa tggctctggt gcgttctggt gaagggggcg
     8641 cttccgcgcc cctgtaagtg gttcgtaatg gcgtaaggct tttgttattg cttgctgctt
     8701 acacctgtaa ggctttcgtt atggtcatgc ctcccatgcg taaaggctga tgatgctgac
     8761 ggtgcggtat cgccctgcct ggtcgcggag ggtagtctgg tactggcctc ggcggttggc
     8821 ggcgcaatga cgcggcttgc caaactggcg gatctcgccg tgttcgatgc tcaggcccgg
     8881 gtagcgggca ggggtggtct gttgtatgct ctgcatgtca ttaaacctct aacgggtggt
     8941 tggtgatgcc cgggttggct ctcacacttc cccgggcctg ggttgcggcg gtgactttag
     9001 cgagtaccac cgccgcgatc tctctcttac tccggcgcga ccgcgtcgac cttctcaaga
     9061 tggcggtaaa tcgcgctccg gcttaggttt agctgtttgg ctatggtggc gatcggcact
     9121 cccatggcgc ggcgcatgac aatctccccg gcttccttcg ctccgatctt ggtcgggcgt
     9181 ccgaacttct ggcctcggct ctttgctgcc tcgatacctg ctgcctggcg ttcgaggatg
     9241 agcttgcgtt ccatctgggc gaagctggcc attacgccga gtaccgcctg tccgacggcg
     9301 ctgctcaggt cgacgccctc gcggatgctg accagggtga tcttccgctt ctccagctcc
     9361 tcgaccagct cgagggtgtc cctggcgttc ctgccgatgc gatcgatcgc gtaaatgacc
     9421 agggtgtcgc cctcctgcag caactggttc aagaggatgc cgagggcagg gcgctcgcgt
     9481 ggtggtaccg tcccgctgat cccgtcgtcg gcaaggatgg aggcggttgg gaaccgggcg
     9541 atgatggcgt ctcgctggct gttgctggtt tgttcgtcgg ttgatacgcg aacgtaaccg
     9601 tatgttttgt tcactttttt ctctcctctg accgatacgt ctctttcgac acaacatgcc
     9661 acaactgtat cgaaagtcta tcgctaaagt gtctttttcg tcgttctgtt gggtgtcctg
     9721 aaatgtaggg gattagcgac ggcctccagg cggctccccg cgctgggcga gagccctgcg
     9781 ccctctgcgt tgatgtctgc actgcgtcgt gacggcatgc tgtctgagcg gcgtttcttg
     9841 ccgctttgct gataacttgc tgagaaccaa accatgaccg aaatcaaaac cgaatgcccg
     9901 gacgcggcct ggctgcgtac cacgcttgct gaaatggcgg gtgatgcgtt ccctgtgtat
     9961 gacctcccgt ccctgcgggt agacccgaac tcctccactc agctttcggc gctcgctgat
    10021 cgtcaggctg cgcgtgaaat gcgtcaggcc gcttccgacg tagaggcgcg acggctggac
    10081 gccgctcgag tggtggaagg gctcaagacc gaggccgaac ggctgcgggg cttgatcgcc
    10141 gacggcaagg ctgcgctgcg tgctggcgag ccggtgagcc ccgacgcggg tgttgcgtcg
    10201 ttcctgctgc cggacatcga ggccgagctg gttgttgctg aggctgctga ggccgacgtg
    10261 gcccgggagc gtgacaccct gctgcaggat gccgatcgcc gtgacgcggc ggctgcgctg
    10321 gcgctgttca actgggcgca ctccgttcgc gtgcagcgta tcgagctgct tctcaagctg
    10381 gcgatggacg aggcgacgac gctcgctgaa gccgacggcg ggcgcggcct gtaccgtacc
    10441 gtgatcgcgc ctgaccgccg tcttaaccaa attttcgcca cgcagggcgc tattgaaatc
    10501 ctgcgccgta accgtggcat ggagggtgtg taatgtctgc tttcaaactc ctgtgtgacc
    10561 ggcttcgcaa tgaggcgacg ctggcaaaat ctgacggggc cggcgctttc aaaaagcaat
    10621 ctctggatcc cgacctgaag ggcggcaaga agggcaaaaa gcccgccggg gagctggagg
    10681 aggacgaggc gatcgcgtct ctccaccgta agcgcatgaa cgacgttaaa ggttgcaaga
    10741 cgctgaccaa atccaccggc gctgatgact ttggcctccc tccgctgtcg tcggtggtcg
    10801 agacagtggt ggagacaggc ggcgcagcgc agacgttcgc gccgatcggg gctccggtgg
    10861 cgtctccgac cgaagcggtt caccgtgcgc gtgcgcagtt catccaggac gcggcgttcc
    10921 tgaacgagat gaaagcggcg ggtgtggcac cggctgacct ggtgttactt gacggcgtgt
    10981 tgacgtcctc tcgtgggatg ccagacgcgg cgcgtgctcc ggcgtttgcc gaggtgcgcc
    11041 gtcaggttca ggcgggcggc atgacgctgt caccggctgc gcgtggtatt gactggaatg
    11101 cgtaagctgc cgcgtcacct gcagggcgag gtgttgatgc cgctgtccag caggatcacg
    11161 cgctcgcaac ggctggagct gatctggctg ttgcccgttc tgcggctcca cccgggcgat
    11221 cgatcggtca ctgagcggcg ggcgcttggc aggttccggg gatcgctcgc tgcgggtggc
    11281 cagacggatg ctttcgtccg ttcggatgcg tctctgtacg cgctgggact gcggttccag
    11341 cgcaagcgca cccgggagct ggttgcttcg gatcccgatg ttgctgcggc tggcgaggcg
    11401 ctggagcagc gtgcaaacgg cgtgaacctg tccgagacgt tcgggggtgg cgatgagtga
    11461 atttgctgtc tcgcgcctgg tcattcctga ggtggtgatc cccgacgggg tcattgctgc
    11521 ccgacgggca aggggacagg ctcaacgtgt cgcagctgct cgccgtgctg cagagcgtcg
    11581 tgctgcgcct acgtggcgct gggtgagacg ggaacagccc cggtcgacag ggcggtgaaa
    11641 cggtgagacg gggcgcgtta tgcgcccttt ttttcgccct tcgcctggcg tgccttgcgc
    11701 cggaatgcct tgatctctgt caggcgggct ttggcgtttt cggcctcctc cggggttgct
    11761 ggttcggtgg ggttgccgtc caggtcgtag cgcatgcact ggtcgatga
//
