LOCUS       NZ_CP073903            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain V1950266 plasmid p2V1950266,
            complete sequence.
ACCESSION   NZ_CP073903
VERSION     NZ_CP073903.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699901
            Assembly: GCF_019330245.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 366x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019330245.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 07:10:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,713
            CDSs (total)                      :: 2,632
            Genes (coding)                    :: 2,552
            CDSs (with protein)               :: 2,552
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 41 of 80
            Pseudo Genes (incomplete)         :: 45 of 80
            Pseudo Genes (internal stop)      :: 27 of 80
            Pseudo Genes (multiple problems)  :: 24 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073903.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="V1950266"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p2V1950266"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(12446..13570,1..798))
                     /locus_tag="KFV55_RS00005"
                     /old_locus_tag="KFV55_00005"
     CDS             complement(join(12446..13570,1..798))
                     /locus_tag="KFV55_RS00005"
                     /old_locus_tag="KFV55_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            1007..1276
                     /locus_tag="KFV55_RS00010"
                     /old_locus_tag="KFV55_00010"
     CDS             1007..1276
                     /locus_tag="KFV55_RS00010"
                     /old_locus_tag="KFV55_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            1293..1532
                     /locus_tag="KFV55_RS00015"
                     /old_locus_tag="KFV55_00015"
     CDS             1293..1532
                     /locus_tag="KFV55_RS00015"
                     /old_locus_tag="KFV55_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1757..2692)
                     /locus_tag="KFV55_RS00020"
                     /old_locus_tag="KFV55_00020"
     CDS             complement(1757..2692)
                     /locus_tag="KFV55_RS00020"
                     /old_locus_tag="KFV55_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            3133..3894
                     /locus_tag="KFV55_RS00025"
                     /old_locus_tag="KFV55_00025"
     CDS             3133..3894
                     /locus_tag="KFV55_RS00025"
                     /old_locus_tag="KFV55_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(4213..4773)
                     /locus_tag="KFV55_RS00035"
                     /old_locus_tag="KFV55_00035"
     CDS             complement(4213..4773)
                     /locus_tag="KFV55_RS00035"
                     /old_locus_tag="KFV55_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(4978..6522)
                     /gene="qac"
                     /locus_tag="KFV55_RS00040"
                     /old_locus_tag="KFV55_00040"
     CDS             complement(4978..6522)
                     /gene="qac"
                     /locus_tag="KFV55_RS00040"
                     /old_locus_tag="KFV55_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            6701..7267
                     /gene="qacR"
                     /locus_tag="KFV55_RS00045"
                     /old_locus_tag="KFV55_00045"
     ARG             6702..7264
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7518..8078
                     /locus_tag="KFV55_RS00050"
                     /old_locus_tag="KFV55_00050"
     CDS             7518..8078
                     /locus_tag="KFV55_RS00050"
                     /old_locus_tag="KFV55_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            8497..9408
                     /locus_tag="KFV55_RS00055"
                     /old_locus_tag="KFV55_00055"
     CDS             8497..9408
                     /locus_tag="KFV55_RS00055"
                     /old_locus_tag="KFV55_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(9585..9683)
                     /locus_tag="KFV55_RS00060"
                     /old_locus_tag="KFV55_00060"
     CDS             complement(9585..9683)
                     /locus_tag="KFV55_RS00060"
                     /old_locus_tag="KFV55_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9847..10545
                     /locus_tag="KFV55_RS00065"
                     /old_locus_tag="KFV55_00065"
     CDS             9847..10545
                     /locus_tag="KFV55_RS00065"
                     /old_locus_tag="KFV55_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10696..11400
                     /locus_tag="KFV55_RS00070"
                     /old_locus_tag="KFV55_00070"
     CDS             10696..11400
                     /locus_tag="KFV55_RS00070"
                     /old_locus_tag="KFV55_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(11449..11682)
                     /locus_tag="KFV55_RS00075"
                     /old_locus_tag="KFV55_00075"
     CDS             complement(11449..11682)
                     /locus_tag="KFV55_RS00075"
                     /old_locus_tag="KFV55_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(11759..12172)
                     /locus_tag="KFV55_RS13650"
                     /old_locus_tag="KFV55_00080"
     CDS             complement(11759..12172)
                     /locus_tag="KFV55_RS13650"
                     /old_locus_tag="KFV55_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     ARG             complement(4982..6522)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          complement(1758..2692)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..798)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="5.87e-175"
ORIGIN
        1 ttgttcattt ttctgtccgt aataagtaac gggttggtac tcgataccct ctttttcaca
       61 gcgttttctt tcattttgtt ctaatctata ttcttctctt gtaagtggaa tttcggcaat
      121 tttttctaaa ccttgcgctt caaaagaacg actatcatat cgtcttaaac tttgttcacg
      181 ttctgagtat tgatttaatt ttaattccca atgcttacga acttctttga aatcaaagtc
      241 ggcaaatcgt tcttgttttc taactttatt accattttta tttaaaatcg cattgccatt
      301 ttcatcatat tgcgttctcg tttttgtctt tttcccccaa gtaccgtctg cattaaacgg
      361 tctttgagta agtagaacat gggcgtgtgg gttgtttata tcatctctat gaattgaaat
      421 atcagcaacc attcctttgt ctacaaactc actttttata aaatctttag ttaattcagt
      481 ttgtacctct ctatcaaaat cattaggcaa acttaataag acttcttttg ctaatctagg
      541 attttttgtt ttcgcattat tcttttcaac tttatcaact tcattccata aacgctgtct
      601 atctttagtc cattcgggtg catgttgagg agttaaaatc atgctttcag gcttaatagt
      661 atgatttcta aacttaattt tttcatctgt acgttcgcta tataatgctt catcagaacg
      721 ataagacgct gacgcaacga tattttttgt tgctcgatta atgttagtca ctctcaacat
      781 aaagtaacct ttattcatga ttttacctcc tctcttttct gtttttttac ccctgtggga
      841 actataatga tcaaccagta tggttgccca tttttatgcc aattttcaat ttgcatgtaa
      901 atgggcagtg cagtgtctta agaactggtt atattatacc atggatgtgc gacttaccca
      961 atgaattatg gtatgcttta tatagataaa ctaagggagt tttttaatgt ctaaaattaa
     1021 acaaacagat attacaaaat taagtcaatt agaaaaacaa attgcacaac tgaaagagca
     1081 aaagaaaaaa caagaagaac aactatctaa taaaattggt ggttattttt taaataaact
     1141 tgatttaaat atgatcaact ctagtgatga attgtataaa attatggata aagtcattgg
     1201 agaatttaat aaagatatcg aacaaaatag cagcaacgag aacaaggaaa atttaacaga
     1261 agacaaaatt aactagaata gtaagtgata cgatgaatga ttttaaacat ctcaaaaaaa
     1321 taaatgctga aattgaaaaa gcagaattaa gaaagcatag attaaaaaat ttagatagaa
     1381 aagaaagagc acatagactg attcgaaaag gtgcaatgct tgaaaagtat tttgaatgtg
     1441 agcatttaag tcctgatgaa acagaagaat tgttaaagat gtattccaac tacattaata
     1501 caaataaacc caataaatat aaaaagaagt aatttatcat gcttacaaat acttgggttt
     1561 cactaaaaag aaaaaattgc tgatagtagt aacaatgaat aataattgtt tataaaaatg
     1621 ggactaaaaa agaaaagttt tagtcccact tacttaatta attacatttc attgaaaata
     1681 aagttcgctt acagttaaga gaacatctaa ttaaagatat tctctaattg gatcattgtt
     1741 ggcttattaa gttgtattat tgattaaata aagaagtgat tggtaaatga tcacgtcgtt
     1801 tattaagcgt atgtacttct gcaaattcag atttaagaga tttcattaag tacccttgca
     1861 ttgcttcaac ggtttcattt ttttgaatta atgtagcttt aaaacgctta agaacattaa
     1921 caatttcaaa ttccatatct tctaataaat aatatgcatc aatagaattg ttgaaggatg
     1981 ttttggcttt taataacaca gatttaataa gtttaatttc agatacagaa aagttactta
     2041 aataactttg aatactttgt ggtagttctt caagtaattg aaattttaaa gcttcatcat
     2101 tgaattttga atagtagtga tttgaatgat ttgtatgatt actagaatat gttaatttcc
     2161 tatcattcaa atcatacata tcattataat tagtcttaat ataatcagtg tcattataat
     2221 cagtatcatt agactgtgaa tttggaagtt cttgaccact taaattcaca gttctagaac
     2281 ttccaaattc attgttctgg aactgtgaat ttggaagttc cttgatatga ctggcttcaa
     2341 tgtcataatt aggttttaat aggtataatc tatttggttt attaaggcca acacgttctt
     2401 gagttagtaa attaaatttt attaactctt tttttaattt tataattttt tgttctgcat
     2461 aacctaagta ttcacacaaa tgtttattac tgaaaataaa gtaaatatta ttatttttat
     2521 ctacccattc attttggagg gataattcaa aacgatcttt taataacgca tacataattt
     2581 tggcttctgc agacattttc ttaaaatatg catcttcaaa taaatattta ggcagttgat
     2641 aaaatctttc agtttttact tgtgataaat tatatttttc aaaattgggc ataataaaaa
     2701 cctcatttcc attttgggat tgaggtaata aaacaatttt tacgtaagtt atatgttttg
     2761 tgcttgcact tagatgatta gatatatata atacaagtat caaaaaaata tataaacaaa
     2821 cgagtgcgaa tcgttttatt accgagagtc gatgctacgc caatagcatc gactctttta
     2881 atatttataa agtaatatta acacttttaa tggcgtatat aaagagtttt aaatgtaatt
     2941 aaaagtacac ctaaaactac caaacgtata attcgctatt ttagcaccta attaagtggc
     3001 gaaaatattt taaggataga taattaggca tttaggtagt taatagatag taaatttagt
     3061 actaaatgat gaaataaatt ttgttaggtg gtaaaattta ggtactaaat taggtaccta
     3121 aggggggatg ttatgaaaac tattaaaatg attgctgatg aattaggtgt aactaaacaa
     3181 actattgtta ataatgctaa aagattaaat atatcttttg aaaaagaaaa tggaattaat
     3241 tatattaaag ataatgatta tttaaaaatt atagaaaaaa tcactaagaa agagaaaaca
     3301 actcataata aagaatcagt aaaaaatgaa ttatttaatg aaaatactgc aaaagttgaa
     3361 aagtatatac ataataattc aaatagttct gagacattaa aaacaaaagt aaacgaatta
     3421 gaaaagcaaa tagaaatctt tgaaactaga gctaaaaatg atgaaaaata tattgagaat
     3481 ctaactaaac aattagatca acaaaatagc aatgttaata cattaaataa actgttggag
     3541 aatcaacaaa tattatcttt agaaaacaat aaaaaaattc aaaaactaga aagccaatta
     3601 gaagaagaaa gacagcttaa atattctttt gatacaacga tgaataatag acagaatatt
     3661 aatccacaag aagcaacttt tactgaagaa tctcaaaata tcaaccaaca acaaaaagaa
     3721 tgtcaaccaa ccgaagtaaa tcataaaaat ataactgaag agaataagca tgacagtaca
     3781 gatgaattaa caaaagagga taagtttgtt gaaaaaacga agaacgattc cagtgataat
     3841 agggaggtag aaccacctaa aaagagattt tggagtcgtt tatttggtaa ctaattgcaa
     3901 ttaaaaaggc acacaagttc atgaacttgt tgtgcctttt taatgtgaat caaacttctg
     3961 ataatcatca gttatgttaa actctaacaa aaactaaatt atcagaagaa ttatctaggg
     4021 tattaaacat aggtaaatca attttctatc gccaatagta taaacatcat ccatattaat
     4081 ctgaagatag ctacataatt ctttaacagc atagccttta tttgtataag gcgccataaa
     4141 ctctattaaa tatggtttgt tttgaggttt ctatgtttaa tcatttaatt tttcattata
     4201 taaatcgaat aattataaaa aatcatcata ctttttattt tttaaaaata tttgaaaatt
     4261 taatagtaat ccaattataa gatcaaatat cattatcatt ataatcaagg caactataat
     4321 cactgtgaca ctcgttacat aacaagctag taatctccag ataaaattga cattttcacc
     4381 aattccataa cttaacatat tacaaagtag taaaagaata ataataccta tactgtaaat
     4441 taaaattgtc agtgtatatt ttttaataac tctattagga aacaatagtt catctattag
     4501 gttctttttc accagtatct ctcgttcaat tctatctttg tcacagattt tgattttact
     4561 attttttatt ttttcctcta ataaaataac ctcgaacgat ctgctaattt ttaataaata
     4621 tgcaggaaaa gctaatgtta agcataatgt aattaattca tttacattgt tcatgtaact
     4681 tataaaatag atgaaagttg ctactagtgc tcctaaaaat atagttatat taagttctac
     4741 atcaaatatt ttaaaaatat ctgctctatt catcagttaa tcatcctttc aaggtttatt
     4801 aatactaata aattattaga tataggtata tcatattatt aatttaagaa attgtctttt
     4861 ataattttac ttaataataa aaaagtagaa ccatttaaaa ttaatggttc tactttttaa
     4921 ctattactaa ttttaaaaat aaactaatct acaatatcta aaaatatatg tttagtacta
     4981 tttttgttta gttatatcaa gtgatttggg aattaacaaa tagacaacta ttgaaatgat
     5041 aatcatgata atcccaccta ctaaagcagt tgctacaaaa gcatcattaa atgatgttac
     5101 agcctcgttt gcaagctgtt ttatccccgt agctttagct acttcgacag cgcccactac
     5161 agattcttca gctacatgag ctaaatctcc aactatacct tttgatgaaa aagatgaaat
     5221 atctaaaaat acacgataaa gcatagaaga taggctacca agtactgcta caccaaaaac
     5281 atttccaagg tcatacatag actcttcaac agcagctgca ttacctgctt ttgatgtagg
     5341 tgtttctaac attattagag cagatgcaac tgctagtgaa gccataccag ctccaactaa
     5401 aattaatgct aaagccattg tagaatatga taatggatga ccaaagaaat acataataaa
     5461 catgccaatg gctgcaattc caattccgga aggtaacact attttcggtc caaatcgcgc
     5521 cgctaatccg ggtgcaattg gtgcaaacac catatctcct attgccatag gtaataggta
     5581 taagccagct ttaaaaggag aaagttcttc cacaacctgt aaccattgtg aagctaataa
     5641 caaaacagat gccattgcaa aaattgtcat aaatgcagca attgtaccag ctgaaaatga
     5701 tctcttttta aaaagtctta cgtctaacat tggatcagaa cttgataaat tacgtttaac
     5761 aaatatcact atcatggtaa ttgctaatac tattacaacc catggaataa tatctgctag
     5821 tccttctttt gaaaattctt tgatactcca taccagtcca atcatgcctg caattgataa
     5881 aattgtagaa ggaatatccc acgagtgaga cttttctttt gataacttag actctggtaa
     5941 taaaaataaa cctgctacaa ctgctattat cgcaaacggt acattaatta aaaatgccga
     6001 gtgccatgaa aattgctcaa gtaaagctcc tccgataatt ggtccaaaaa cagcacctat
     6061 cgatgaagcg attgaccata cagctaatgc agtggccctt tctttagggt tttcaaaaat
     6121 tactcttatc attgaaagag tagttggcat tattaaagca cctgcaatac caagtaaaaa
     6181 tcgaatagct attacgaact ctgcactttc tgcgaaaaat atagctaatg aaacgaggcc
     6241 aaataaagca aatccagtta ataatgcttt ttttcttccc catttatcag caaaggcact
     6301 caatggaatt ataaagccag ctaaaacaag agagtatata tcaactatcc ataactgttg
     6361 ggtaccagaa ggctctaact ctcttactaa ttccggtaaa gccataatta atattgtcat
     6421 atccattgta acaacaaaca aactaacagc taatactact agtgcagtcc atcttttttt
     6481 tgatgtcatc atatcagtag tttttgtaaa aaatgaaatc atttaagttc cctccaatcc
     6541 ttatagaccg tgcgatcggt ctataaggat tataatctat tttttttaca tattacaaca
     6601 aaaaatcttg cgaaatttaa atggctgtat tgtgttatac aattattctt tgaatttttt
     6661 gtgctatcat tgatagtact taatactcat taaaggcgtg atgaacttga aagataaaat
     6721 actaggtgtc gcaaaggaat tatttataaa aaatggatat aatgccacta ctactggaga
     6781 aattgttaaa ttatcagaaa gtagtaaagg gaatctttat tatcacttta aaacaaaaga
     6841 aaatctattt ttagaaattt taaatataga agaatctaaa tggcaagaac agtggaaaaa
     6901 ggaacaaatc aaatgtaaaa ctaatagaga aaaattttat ttatataatg aactatcttt
     6961 aacaaccgaa tactattatc cacttcaaaa cgcaataatt gaattttaca ctgaatatta
     7021 taaaactaat agcattaatg aaaaaatgaa taaattagaa aacaaatata tagatgctta
     7081 tcatgtaatt tttaaggagg gtaatttaaa tggcgaatgg tgtattaatg atgttaatgc
     7141 tgttagtaaa atagcagcaa acgctgttaa tggaattgtt acctttacac atgaacaaaa
     7201 tattaatgaa agaattaaac ttatgaacaa gttctctcaa atttttttaa atggacttag
     7261 taaataatta tatatatatt cttgaaatac caataaatat tatttctaaa ttacgttcgt
     7321 ttttgacaca aaacctctta acttaatatt aagaggtttt tcttggatat ttaagtctat
     7381 aaacttgtgt tataatcaat gtatcataaa tatcgaaaat catgagtttt tagatgctga
     7441 cagacgtgtt tatttacatc gagaactata tttcattagt aagtacccat aaaacgatta
     7501 aaaaaggtgg tgttcaaatg gctaaaattg gttatgcacg tgtgtcaaca caagatcaaa
     7561 gtcttgatgg acagattgat acacttgagg aatatggttg tgaacgtatc tttagcgaga
     7621 aagcgagtgg tcgtaaaact aaaagaacgg aacttgataa gtgtttagat tatttacgtg
     7681 aaggagatat tctagttgtc tataaattag atcgtcttgg acgtacaaca aaacaattaa
     7741 ttgagctatc tcaatggttg gatgaaaata gcattgattt acatattata gatatgaatg
     7801 tgtcaacgaa agatgcgatg ggcaagatgt tctttaccat gatgagtgca ttcgctgaac
     7861 ttgaagctaa tttattaagt gaacgaacta aaaaaggttt ggaagctgca agagcaagag
     7921 ggagaaaagg tggaagacct tcactaccag atcataagaa aagagagatc aaattcttat
     7981 atgatgaaca aaagctgtct ggtgaagaaa ttgctgaaca aacaggagtg agtcgttcta
     8041 ctgtatatag gattattaaa gagtcaagaa aaatataaag tactaaatta aagttttaat
     8101 atacccttta attgaaaaat cttcttaaaa agtgtcttta acttcagtcc cctttaacaa
     8161 tccactcgct taatagcttt aaatttctaa gcattacttg agctttgaaa attttcatcg
     8221 attcattaac cgacataact ggattttgac tgttaatatt caccttgtcc tctccaatta
     8281 acaaaaaagc aaaagcatat aaacaacatt tgttgtttat atgcttttgc ttgcattttc
     8341 ttctatagta tgctataatg aaaaggttaa gtctatctat acatcaaaag caataaactt
     8401 ccacctagaa taacaactta ttgtaaatcg acaattacaa taatacatca ttctatattg
     8461 gaagtaaaca aaaaacttac agaaggatga tatagagtga cactagtatg taccaaattc
     8521 aatcatgatg ctaaatcaac tgattattta aatatttcta ttgaagaaga cactcttcaa
     8581 tatatagacc caaaccttat taaagtaata tctaatagag aagttggaaa agttgcgact
     8641 ttggctcaaa gagcacaaga gcaaattgat gttttttttg aaaaaattat cgatatacat
     8701 cttagtgatt tttctgatac ccaaaagcgt aatgaattta gaaaattatt ctctcatttt
     8761 tcagaaccac accatttacg cttaggtttt tctcagaaag gtaatacagg aaaaggtact
     8821 acagcatctg aattgattaa tctatttatg aatgaagata ttctttccat tatcttaaat
     8881 gatgacggat taacaatacc tcaaaaagca ccattaatca gaaattttgg tgatgataaa
     8941 ttatcagatt taacaagtaa tataatcatg aatgttataa ttgattttaa taaatccatg
     9001 attcagtcat ttccagaaat gaaatcgttt ctttctaaag aaactgtgac atattattat
     9061 ttctctacta acggtatatg ggaaacttat gatttcactc catttctttt tgatggtaat
     9121 aaaactttat tagttccaaa attattcact acttataatc agacatctag tttagatttg
     9181 atgataagag tatacattga agaagaaatc attagattgc aaaaacaacg taatacaacc
     9241 aaaaaaatga caaaaaaaca atttatcaat gaatttataa aaggacatcg tatggataat
     9301 atgacctatt tctttttaaa cacttcttca aaatcacaca aaaaatttca aaaagaatta
     9361 gattcaaaag ccaatgaaag gggtaacaat aacaaagatg ataattagta cttactaatc
     9421 agtatagtat agacttaaca tactaattat tcaattttgc attcctccac actatgatgt
     9481 tataatttag ataacaaata tgtgttgaac atggtaaaca ccgagcccga taactattcc
     9541 agtagttatc gggctctttt tggtgttatt actgtgtcgt cgctttattg tttatcacga
     9601 ttattcaacc aatgtgaaaa caacgtgatt ataaccccta ctataatagg agctactata
     9661 gatacaaata tgatgctgaa catggtgtca cctccttcca tgctaaacca gcacagaagt
     9721 agaacgacga cttttatatt atatcatttg tgaattgtta agcatatgca cttccgataa
     9781 gtcattttat gtttaatgta tctatgtgat aatgaaaata caaattctat taaaggagat
     9841 aattttatga aaaaacgatc attacttcca actttattat tggctagctc attaacattc
     9901 acaggttttt cagcgacagc caatgctgag gatggtgcca aaaccgtata cggagagaac
     9961 ttaccaaata gcgcagatac aaatggagat ggttgggcta atgtagactt caatacttca
    10021 tccctcccac aatctgcaca aaatcaaata agtgaacttt ctaaacagaa agattcaggg
    10081 gaattaactc aaatcgagta taatgagaaa gtttcaagtg tatttaatga atatcaatca
    10141 caaagtactg atatacaaaa taatgagaga actcaacaag gtaaaagacc tttcggaggg
    10201 gtgataccta attgtatgac tcaagaagaa taccaagaat tagaaagtca tgttcctaac
    10261 cctaatagtg tatccacaga aaaatataat caaattgtaa aaaatgaaag tcaaaagata
    10321 gctaatgaag aaaataaaac tattaataca cctcaaggtg tgttgacacc tgtaactgaa
    10381 aataaaaaca agagttctga aggaatgaaa aatcaagatt caaactctca agtattacca
    10441 gaaacaggag aaagcaacca aagtgatata ttgtcaataa ttggcgcttc tggactacta
    10501 atattaggta taagtctttt aatattcaga aaaaaggcaa tataaaaatt gataaaataa
    10561 ccaccgtgtt tatgtaaaac atgtggttat tttattatat atttaataca tatttttcaa
    10621 ctcatgtaat ttacgttttc ttgctgaaaa aatattaaca taatctaatt tgaaatactg
    10681 aaaaggagtt gtaatatgaa aaaaatcatt ttacctatat taattgttag tttatcggta
    10741 attttagtga gttgtaataa taacgattca gaacatcaca atgataataa ttctagtgat
    10801 actaacacct cagaaaacaa agataatgaa gcatcaaatg aaattgacga aaaaactaac
    10861 acatctgata gtgcggataa ttcagaaagt agtaataaac aaaataaaga aaaaaatgat
    10921 aataaaaaac aagaaaaaaa taatgatagt aataagaagc aacaatctac tgataaaaat
    10981 aaaagttact taaataattt taactcgaaa gaaattgaat atgcacgagt ttggtatcag
    11041 ttaatatcta caagaaatga tttaaaaggt attaaaaatg tttatgttac agaaatacca
    11101 aaaggctcaa aagtaaatcc acaagccaaa aatagtgcaa tttataaaga aaaagttgtg
    11161 aaattagaag ctcctatgag agctggtggg tctgtaacat atagcagtaa tggtgatgga
    11221 acaattaatg tatataataa tatcccttat aaatgggaaa attcacaaaa tagtgattat
    11281 agtcaaatga ataagataac aaaaaaagca atagaggaga atattgaaac agtctatatt
    11341 aaaccctacg acaataaaac agtagctaag ttagcaagta aaattagata taataagtag
    11401 ttagtagatt attaaaaagg cgcctaaaat taggcgtctt ttttatcatt agttcaaaag
    11461 ataagcataa cgttccatgt aacagcatat aaagtaatga atcaattcat cactatctaa
    11521 tgccaccatt gttagttcag ccttgcatgg tttaacgtat ctcacataat ggtacaaatc
    11581 ttcaaatagt gcctgttttt cgggcatttt aaacaccttg tgtacattaa aacctatttc
    11641 ttctaatcgc tttgtaatca cgccgaaaac gaatgtgttc atgtgaaatc actccatcat
    11701 ttttaatatt cacatgtaca ctaaaaaaaa tccgatacat cggcttttta tttttggttt
    11761 aatttatttc tatattgttt tcactatcgc tcggacgcat aagtgttaaa acaccttcta
    11821 ccatacctat cacacccggt attcccgtcc aacaaaatag taaataaaga agagcatttt
    11881 tatttttctt aacataaaac ttatgagccc cgaaacctcc tagaaaaaat gctaatgcag
    11941 agtaaacata tttattaact tgcatgattt ccactccttt aattaattga tttagagaaa
    12001 cattaaataa tttagtgatt tctaaaattg tttttaagtc gggattactt acttctcttt
    12061 cccaattaga aatagaatgt ctcgataccc ctaacttttc agctaatttt tcttgagaca
    12121 tgtttttact ttttcttaac ttttgtattt gatgtccaat ttttgtttcc aaattatttt
    12181 tctccttaac tttaagatat atttaaactc aattttttct atcgatattt atgaacatta
    12241 gtatttttta tgctgaaata tgtgaacaat cgcataatat caaggttttt ctataattat
    12301 acacgtttta aaatacattg tgataataca tagaatgtac cttatctaag tatataaatg
    12361 ttttctttaa accaaagcta atttaccaca ttgggttaag tttcttatta ttaaagaatg
    12421 aactttgtat gatttcaact gcttactatc ttctcatttg gttatttctc tcttgtttct
    12481 tcttcttttt ctgatgatta atattgtttt gcttttcagt ttcgttcgca tagtacataa
    12541 gaaagtcact agcatttatc gttggtaaat taatgtgatt agtttgttca ttttcatgtt
    12601 caatacgatt gtcatgatca tttctatcta cttgattaat atgtttttcg ataacttctt
    12661 cttgatcgac atgattatat ttctcaagtt taatgagtaa ttgaactttt tcattaggtg
    12721 tttggtcttt gactttttca aagtttttat cgcctatatc tattcttaaa tcatcatcaa
    12781 tatgttcttt ataactatca atttcttttt ctttttcctt aataaattgc aactcaacgt
    12841 tatattcaaa gctatctttt tctaaatttt taagatgttt ttgactaaag aaaatatctt
    12901 tactcgcttt tttataagta tcttctagca atacatcatt aggaatattg ttattttgtt
    12961 tgaacaggta ttgaacatga gttgctttta taatattacc tttttgtgcc tcatcaacaa
    13021 taaatgcttt ttcatcatgg ctaaaatgat gatgagattt agggtacatt tgctgaacaa
    13081 tgagatgatt tttttcactt tcagttagat aaacatcttt agctgattga taaatctctt
    13141 tacgtttttc ctctctactt tctaatttat tcttctcatt gagtaacgct ttatagttag
    13201 tttcaacagt attaacaaac tcttgttgac tatatccttt actttcaatt tcttttctaa
    13261 actgataatt ttcatcataa tttttagcat agaattttaa ttgacgtctc ttaatttcat
    13321 ttttaagtaa atcattttga attttacgac caaaggtaga cgcactgtca aacgtatttt
    13381 taattgtttc tttcgcctct gaatagccaa catcacgttg atatctatca aataaaatag
    13441 aataactttg ttcattttta cttacttgtt cgttagcatt ttgaataaat aatctaattt
    13501 cattttgtgc ttgttctttc tctttatcac taaataattg tgatacttcg ccacgattat
    13561 atcttctaat
//
