LOCUS       NZ_CP073902            13570 bp    DNA     circular CON 16-APR-2024
DEFINITION  Staphylococcus epidermidis strain V1949610 plasmid p1V1949610,
            complete sequence.
ACCESSION   NZ_CP073902
VERSION     NZ_CP073902.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699900
            Assembly: GCF_019330225.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 458x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019330225.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 08:09:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,679
            CDSs (total)                      :: 2,598
            Genes (coding)                    :: 2,519
            CDSs (with protein)               :: 2,519
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 40 of 79
            Pseudo Genes (incomplete)         :: 44 of 79
            Pseudo Genes (internal stop)      :: 28 of 79
            Pseudo Genes (multiple problems)  :: 24 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073902.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="V1949610"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1V1949610"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13274..13570,1..270))
                     /gene="qacR"
                     /locus_tag="KFV25_RS13215"
                     /old_locus_tag="KFV25_13230"
     CDS             complement(join(13274..13570,1..270))
                     /gene="qacR"
                     /locus_tag="KFV25_RS13215"
                     /old_locus_tag="KFV25_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug-binding transcriptional regulator QacR"
                     /protein_id="WP_001807342.1"
                     /translation="MNLKDKILGVAKELFIKNGYNATTTGEIVKLSESSKGNLYYHFKT
                     KENLFLEILNIEESKWQEQWKKEQIKCKTNREKFYLYNELSLTTEYYYPLQNAIIEFYT
                     EYYKTNSINEKMNKLENKYIDAYHVIFKEGNLNGEWCINDVNAVSKIAANAVNGIVTFT
                     HEQNINERIKLMNKFSQIFLNGLSK"
                     /name="qacR"
     gene            449..1993
                     /gene="qac"
                     /locus_tag="KFV25_RS13220"
                     /old_locus_tag="KFV25_13235"
     CDS             449..1993
                     /gene="qac"
                     /locus_tag="KFV25_RS13220"
                     /old_locus_tag="KFV25_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            2198..2758
                     /locus_tag="KFV25_RS13225"
                     /old_locus_tag="KFV25_13240"
     CDS             2198..2758
                     /locus_tag="KFV25_RS13225"
                     /old_locus_tag="KFV25_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(3077..3838)
                     /locus_tag="KFV25_RS13235"
                     /old_locus_tag="KFV25_13250"
     CDS             complement(3077..3838)
                     /locus_tag="KFV25_RS13235"
                     /old_locus_tag="KFV25_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            4279..5214
                     /locus_tag="KFV25_RS13240"
                     /old_locus_tag="KFV25_13255"
     CDS             4279..5214
                     /locus_tag="KFV25_RS13240"
                     /old_locus_tag="KFV25_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(5439..5678)
                     /locus_tag="KFV25_RS13245"
                     /old_locus_tag="KFV25_13260"
     CDS             complement(5439..5678)
                     /locus_tag="KFV25_RS13245"
                     /old_locus_tag="KFV25_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(5695..5964)
                     /locus_tag="KFV25_RS13250"
                     /old_locus_tag="KFV25_13265"
     CDS             complement(5695..5964)
                     /locus_tag="KFV25_RS13250"
                     /old_locus_tag="KFV25_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            6173..8095
                     /locus_tag="KFV25_RS13255"
                     /old_locus_tag="KFV25_13270"
     CDS             6173..8095
                     /locus_tag="KFV25_RS13255"
                     /old_locus_tag="KFV25_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            8369..8782
                     /locus_tag="KFV25_RS13525"
                     /old_locus_tag="KFV25_13275"
     CDS             8369..8782
                     /locus_tag="KFV25_RS13525"
                     /old_locus_tag="KFV25_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            8859..9092
                     /locus_tag="KFV25_RS13265"
                     /old_locus_tag="KFV25_13280"
     CDS             8859..9092
                     /locus_tag="KFV25_RS13265"
                     /old_locus_tag="KFV25_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(9141..9845)
                     /locus_tag="KFV25_RS13270"
                     /old_locus_tag="KFV25_13285"
     CDS             complement(9141..9845)
                     /locus_tag="KFV25_RS13270"
                     /old_locus_tag="KFV25_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(9996..10694)
                     /locus_tag="KFV25_RS13275"
                     /old_locus_tag="KFV25_13290"
     CDS             complement(9996..10694)
                     /locus_tag="KFV25_RS13275"
                     /old_locus_tag="KFV25_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10858..10956
                     /locus_tag="KFV25_RS13280"
                     /old_locus_tag="KFV25_13295"
     CDS             10858..10956
                     /locus_tag="KFV25_RS13280"
                     /old_locus_tag="KFV25_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(11133..12044)
                     /locus_tag="KFV25_RS13285"
                     /old_locus_tag="KFV25_13300"
     CDS             complement(11133..12044)
                     /locus_tag="KFV25_RS13285"
                     /old_locus_tag="KFV25_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(12463..13023)
                     /locus_tag="KFV25_RS13290"
                     /old_locus_tag="KFV25_13305"
     CDS             complement(12463..13023)
                     /locus_tag="KFV25_RS13290"
                     /old_locus_tag="KFV25_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     ARG             450..1990
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          4280..5214
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6174..8026
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 ttcggttgtt aaagatagtt cattatataa ataaaatttt tctctattag ttttacattt
       61 gatttgttcc tttttccact gttcttgcca tttagattct tctatattta aaatttctaa
      121 aaatagattt tcttttgttt taaagtgata ataaagattc cctttactac tttctgataa
      181 tttaacaatt tctccagtag tagtggcatt atatccattt tttataaata attcctttgc
      241 gacacctagt attttatctt tcaagttcat cacgccttta atgagtatta agtactatca
      301 atgatagcac aaaaaattca aagaataatt gtataacaca atacagccat ttaaatttcg
      361 caagattttt tgttgtaata tgtaaaaaaa atagattata atccttatag accgatcgca
      421 cggtctataa ggattggagg gaacttaaat gatttcattt tttacaaaaa ctactgatat
      481 gatgacatca aaaaaaagat ggactgcact agtagtatta gctgttagtt tgtttgttgt
      541 tacaatggat atgacaatat taattatggc tttaccggaa ttagtaagag agttagagcc
      601 ttctggtacc caacagttat ggatagttga tatatactct cttgttttag ctggctttat
      661 aattccattg agtgcctttg ctgataaatg gggaagaaaa aaagcattat taactggatt
      721 tgctttattt ggcctcgttt cattagctat atttttcgca gaaagtgcag agttcgtaat
      781 agctattcga tttttacttg gtattgcagg tgctttaata atgccaacta ctctttcaat
      841 gataagagta atttttgaaa accctaaaga aagggccact gcattagctg tatggtcaat
      901 cgcttcatcg ataggtgctg tttttggacc aattatcgga ggagctttac ttgagcaatt
      961 ttcatggcac tcggcatttt taattaatgt accgtttgcg ataatagcag ttgtagcagg
     1021 tttattttta ttaccagagt ctaagttatc aaaagaaaag tctcactcgt gggatattcc
     1081 ttctacaatt ttatcaattg caggcatgat tggactggta tggagtatca aagaattttc
     1141 aaaagaagga ctagcagata ttattccatg ggttgtaata gtattagcaa ttaccatgat
     1201 agtgatattt gttaaacgta atttatcaag ttctgatcca atgttagacg taagactttt
     1261 taaaaagaga tcattttcag ctggtacaat tgctgcattt atgacaattt ttgcaatggc
     1321 atctgttttg ttattagctt cacaatggtt acaggttgtg gaagaacttt ctccttttaa
     1381 agctggctta tacctattac ctatggcaat aggagatatg gtgtttgcac caattgcacc
     1441 cggattagcg gcgcgatttg gaccgaaaat agtgttacct tccggaattg gaattgcagc
     1501 cattggcatg tttattatgt atttctttgg tcatccatta tcatattcta caatggcttt
     1561 agcattaatt ttagttggag ctggtatggc ttcactagca gttgcatctg ctctaataat
     1621 gttagaaaca cctacatcaa aagcaggtaa tgcagctgct gttgaagagt ctatgtatga
     1681 ccttggaaat gtttttggtg tagcagtact tggtagccta tcttctatgc tttatcgtgt
     1741 atttttagat atttcatctt tttcatcaaa aggtatagtt ggagatttag ctcatgtagc
     1801 tgaagaatct gtagtgggcg ctgtcgaagt agctaaagct acggggataa aacagcttgc
     1861 aaacgaggct gtaacatcat ttaatgatgc ttttgtagca actgctttag taggtgggat
     1921 tatcatgatt atcatttcaa tagttgtcta tttgttaatt cccaaatcac ttgatataac
     1981 taaacaaaaa tagtactaaa catatatttt tagatattgt agattagttt atttttaaaa
     2041 ttagtaatag ttaaaaagta gaaccattaa ttttaaatgg ttctactttt ttattattaa
     2101 gtaaaattat aaaagacaat ttcttaaatt aataatatga tatacctata tctaataatt
     2161 tattagtatt aataaacctt gaaaggatga ttaactgatg aatagagcag atatttttaa
     2221 aatatttgat gtagaactta atataactat atttttagga gcactagtag caactttcat
     2281 ctattttata agttacatga acaatgtaaa tgaattaatt acattatgct taacattagc
     2341 ttttcctgca tatttattaa aaattagcag atcgttcgag gttattttat tagaggaaaa
     2401 aataaaaaat agtaaaatca aaatctgtga caaagataga attgaacgag agatactggt
     2461 gaaaaagaac ctaatagatg aactattgtt tcctaataga gttattaaaa aatatacact
     2521 gacaatttta atttacagta taggtattat tattctttta ctactttgta atatgttaag
     2581 ttatggaatt ggtgaaaatg tcaattttat ctggagatta ctagcttgtt atgtaacgag
     2641 tgtcacagtg attatagttg ccttgattat aatgataatg atatttgatc ttataattgg
     2701 attactatta aattttcaaa tatttttaaa aaataaaaag tatgatgatt ttttataatt
     2761 attcgattta tataatgaaa aattaaatga ttaaacatag aaacctcaaa acaaaccata
     2821 tttaatagag tttatggcgc cttatacaaa taaaggctat gctgttaaag aattatgtag
     2881 ctatcttcag attaatatgg atgatgttta tactattggc gatagaaaat tgatttacct
     2941 atgtttaata ccctagataa ttcttctgat aatttagttt ttgttagagt ttaacataac
     3001 tgatgattat cagaagtttg attcacatta aaaaggcaca acaagttcat gaacttgtgt
     3061 gcctttttaa ttgcaattag ttaccaaata aacgactcca aaatctcttt ttaggtggtt
     3121 ctacctccct attatcactg gaatcgttct tcgttttttc aacaaactta tcctcttttg
     3181 ttaattcatc tgtactgtca tgcttattct cttcagttat atttttatga tttacttcgg
     3241 ttggttgaca ttctttttgt tgttggttga tattttgaga ttcttcagta aaagttgctt
     3301 cttgtggatt aatattctgt ctattattca tcgttgtatc aaaagaatat ttaagctgtc
     3361 tttcttcttc taattggctt tctagttttt gaattttttt attgttttct aaagataata
     3421 tttgttgatt ctccaacagt ttatttaatg tattaacatt gctattttgt tgatctaatt
     3481 gtttagttag attctcaata tatttttcat catttttagc tctagtttca aagatttcta
     3541 tttgcttttc taattcgttt acttttgttt ttaatgtctc agaactattt gaattattat
     3601 gtatatactt ttcaactttt gcagtatttt cattaaataa ttcatttttt actgattctt
     3661 tattatgagt tgttttctct ttcttagtga ttttttctat aatttttaaa taatcattat
     3721 ctttaatata attaattcca ttttcttttt caaaagatat atttaatctt ttagcattat
     3781 taacaatagt ttgtttagtt acacctaatt catcagcaat cattttaata gttttcataa
     3841 catcccccct taggtaccta atttagtacc taaattttac cacctaacaa aatttatttc
     3901 atcatttagt actaaattta ctatctatta actacctaaa tgcctaatta tctatcctta
     3961 aaatattttc gccacttaat taggtgctaa aatagcgaat tatacgtttg gtagttttag
     4021 gtgtactttt aattacattt aaaactcttt atatacgcca ttaaaagtgt taatattact
     4081 ttataaatat taaaagagtc gatgctattg gcgtagcatc gactctcggt aataaaacga
     4141 ttcgcactcg tttgtttata tatttttttg atacttgtat tatatatatc taatcatcta
     4201 agtgcaagca caaaacatat aacttacgta aaaattgttt tattacctca atcccaaaat
     4261 ggaaatgagg tttttattat gcccaatttt gaaaaatata atttatcaca agtaaaaact
     4321 gaaagatttt atcaactgcc taaatattta tttgaagatg catattttaa gaaaatgtct
     4381 gcagaagcca aaattatgta tgcgttatta aaagatcgtt ttgaattatc cctccaaaat
     4441 gaatgggtag ataaaaataa taatatttac tttattttca gtaataaaca tttgtgtgaa
     4501 tacttaggtt atgcagaaca aaaaattata aaattaaaaa aagagttaat aaaatttaat
     4561 ttactaactc aagaacgtgt tggccttaat aaaccaaata gattatacct attaaaacct
     4621 aattatgaca ttgaagccag tcatatcaag gaacttccaa attcacagtt ccagaacaat
     4681 gaatttggaa gttctagaac tgtgaattta agtggtcaag aacttccaaa ttcacagtct
     4741 aatgatactg attataatga cactgattat attaagacta attataatga tatgtatgat
     4801 ttgaatgata ggaaattaac atattctagt aatcatacaa atcattcaaa tcactactat
     4861 tcaaaattca atgatgaagc tttaaaattt caattacttg aagaactacc acaaagtatt
     4921 caaagttatt taagtaactt ttctgtatct gaaattaaac ttattaaatc tgtgttatta
     4981 aaagccaaaa catccttcaa caattctatt gatgcatatt atttattaga agatatggaa
     5041 tttgaaattg ttaatgttct taagcgtttt aaagctacat taattcaaaa aaatgaaacc
     5101 gttgaagcaa tgcaagggta cttaatgaaa tctcttaaat ctgaatttgc agaagtacat
     5161 acgcttaata aacgacgtga tcatttacca atcacttctt tatttaatca ataatacaac
     5221 ttaataagcc aacaatgatc caattagaga atatctttaa ttagatgttc tcttaactgt
     5281 aagcgaactt tattttcaat gaaatgtaat taattaagta agtgggacta aaacttttct
     5341 tttttagtcc catttttata aacaattatt attcattgtt actactatca gcaatttttt
     5401 ctttttagtg aaacccaagt atttgtaagc atgataaatt acttcttttt atatttattg
     5461 ggtttatttg tattaatgta gttggaatac atctttaaca attcttctgt ttcatcagga
     5521 cttaaatgct cacattcaaa atacttttca agcattgcac cttttcgaat cagtctatgt
     5581 gctctttctt ttctatctaa attttttaat ctatgctttc ttaattctgc tttttcaatt
     5641 tcagcattta tttttttgag atgtttaaaa tcattcatcg tatcacttac tattctagtt
     5701 aattttgtct tctgttaaat tttccttgtt ctcgttgctg ctattttgtt cgatatcttt
     5761 attaaattct ccaatgactt tatccataat tttatacaat tcatcactag agttgatcat
     5821 atttaaatca agtttattta aaaaataacc accaatttta ttagatagtt gttcttcttg
     5881 ttttttcttt tgctctttca gttgtgcaat ttgtttttct aattgactta attttgtaat
     5941 atctgtttgt ttaattttag acattaaaaa actcccttag tttatctata taaagcatac
     6001 cataattcat tgggtaagtc gcacatccat ggtataatat aaccagttct taagacactg
     6061 cactgcccat ttacatgcaa attgaaaatt ggcataaaaa tgggcaacca tactggttga
     6121 tcattatagt tcccacaggg gtaaaaaaac agaaaagaga ggaggtaaaa tcatgaataa
     6181 aggttacttt atgttgagag tgactaacat taatcgagca acaaaaaata tcgttgcgtc
     6241 agcgtcttat cgttctgatg aagcattata tagcgaacgt acagatgaaa aaattaagtt
     6301 tagaaatcat actattaagc ctgaaagcat gattttaact cctcaacatg cacccgaatg
     6361 gactaaagat agacagcgtt tatggaatga agttgataaa gttgaaaaga ataatgcgaa
     6421 aacaaaaaat cctagattag caaaagaagt cttattaagt ttgcctaatg attttgatag
     6481 agaggtacaa actgaattaa ctaaagattt tataaaaagt gagtttgtag acaaaggaat
     6541 ggttgctgat atttcaattc atagagatga tataaacaac ccacacgccc atgttctact
     6601 tactcaaaga ccgtttaatg cagacggtac ttgggggaaa aagacaaaaa cgagaacgca
     6661 atatgatgaa aatggcaatg cgattttaaa taaaaatggt aataaagtta gaaaacaaga
     6721 acgatttgcc gactttgatt tcaaagaagt tcgtaagcat tgggaattaa aattaaatca
     6781 atactcagaa cgtgaacaaa gtttaagacg atatgatagt cgttcttttg aagcgcaagg
     6841 tttagaaaaa attgccgaaa ttccacttac aagagaagaa tatagattag aacaaaatga
     6901 aagaaaacgc tgtgaaaaag agggtatcga gtaccaaccc gttacttatt acggacagaa
     6961 aaatgaacaa attagaagat ataatcgtgg cgaagtatca caattattta gtgataaaga
     7021 gaaagaacaa gcacaaaatg aaattagatt atttattcaa aatgctaacg aacaagtaag
     7081 taaaaatgaa caaagttatt ctattttatt tgatagatat caacgtgatg ttggctattc
     7141 agaggcgaaa gaaacaatta aaaatacgtt tgacagtgcg tctacctttg gtcgtaaaat
     7201 tcaaaatgat ttacttaaaa atgaaattaa gagacgtcaa ttaaaattct atgctaaaaa
     7261 ttatgatgaa aattatcagt ttagaaaaga aattgaaagt aaaggatata gtcaacaaga
     7321 gtttgttaat actgttgaaa ctaactataa agcgttactc aatgagaaga ataaattaga
     7381 aagtagagag gaaaaacgta aagagattta tcaatcagct aaagatgttt atctaactga
     7441 aagtgaaaaa aatcatctca ttgttcagca aatgtaccct aaatctcatc atcattttag
     7501 ccatgatgaa aaagcattta ttgttgatga ggcacaaaaa ggtaatatta taaaagcaac
     7561 tcatgttcaa tacctgttca aacaaaataa caatattcct aatgatgtat tgctagaaga
     7621 tacttataaa aaagcgagta aagatatttt ctttagtcaa aaacatctta aaaatttaga
     7681 aaaagatagc tttgaatata acgttgagtt gcaatttatt aaggaaaaag aaaaagaaat
     7741 tgatagttat aaagaacata ttgatgatga tttaagaata gatataggcg ataaaaactt
     7801 tgaaaaagtc aaagaccaaa cacctaatga aaaagttcaa ttactcatta aacttgagaa
     7861 atataatcat gtcgatcaag aagaagttat cgaaaaacat attaatcaag tagatagaaa
     7921 tgatcatgac aatcgtattg aacatgaaaa tgaacaaact aatcacatta atttaccaac
     7981 gataaatgct agtgactttc ttatgtacta tgcgaacgaa actgaaaagc aaaacaatat
     8041 taatcatcag aaaaagaaga agaaacaaga gagaaataac caaatgagaa gatagtaagc
     8101 agttgaaatc atacaaagtt cattctttaa taataagaaa cttaacccaa tgtggtaaat
     8161 tagctttggt ttaaagaaaa catttatata cttagataag gtacattcta tgtattatca
     8221 caatgtattt taaaacgtgt ataattatag aaaaaccttg atattatgcg attgttcaca
     8281 tatttcagca taaaaaatac taatgttcat aaatatcgat agaaaaaatt gagtttaaat
     8341 atatcttaaa gttaaggaga aaaataattt ggaaacaaaa attggacatc aaatacaaaa
     8401 gttaagaaaa agtaaaaaca tgtctcaaga aaaattagct gaaaagttag gggtatcgag
     8461 acattctatt tctaattggg aaagagaagt aagtaatccc gacttaaaaa caattttaga
     8521 aatcactaaa ttatttaatg tttctctaaa tcaattaatt aaaggagtgg aaatcatgca
     8581 agttaataaa tatgtttact ctgcattagc attttttcta ggaggtttcg gggctcataa
     8641 gttttatgtt aagaaaaata aaaatgctct tctttattta ctattttgtt ggacgggaat
     8701 accgggtgtg ataggtatgg tagaaggtgt tttaacactt atgcgtccga gcgatagtga
     8761 aaacaatata gaaataaatt aaaccaaaaa taaaaagccg atgtatcgga ttttttttag
     8821 tgtacatgtg aatattaaaa atgatggagt gatttcacat gaacacattc gttttcggcg
     8881 tgattacaaa gcgattagaa gaaataggtt ttaatgtaca caaggtgttt aaaatgcccg
     8941 aaaaacaggc actatttgaa gatttgtacc attatgtgag atacgttaaa ccatgcaagg
     9001 ctgaactaac aatggtggca ttagatagtg atgaattgat tcattacttt atatgctgtt
     9061 acatggaacg ttatgcttat cttttgaact aatgataaaa aagacgccta attttaggcg
     9121 cctttttaat aatctactaa ctacttatta tatctaattt tacttgctaa cttagctact
     9181 gttttattgt cgtagggttt aatatagact gtttcaatat tctcctctat tgcttttttt
     9241 gttatcttat tcatttgact ataatcacta ttttgtgaat tttcccattt ataagggata
     9301 ttattatata cattaattgt tccatcacca ttactgctat atgttacaga cccaccagct
     9361 ctcataggag cttctaattt cacaactttt tctttataaa ttgcactatt tttggcttgt
     9421 ggatttactt ttgagccttt tggtatttct gtaacataaa catttttaat accttttaaa
     9481 tcatttcttg tagatattaa ctgataccaa actcgtgcat attcaatttc tttcgagtta
     9541 aaattattta agtaactttt atttttatca gtagattgtt gcttcttatt actatcatta
     9601 tttttttctt gttttttatt atcatttttt tctttatttt gtttattact actttctgaa
     9661 ttatccgcac tatcagatgt gttagttttt tcgtcaattt catttgatgc ttcattatct
     9721 ttgttttctg aggtgttagt atcactagaa ttattatcat tgtgatgttc tgaatcgtta
     9781 ttattacaac tcactaaaat taccgataaa ctaacaatta atataggtaa aatgattttt
     9841 ttcatattac aactcctttt cagtatttca aattagatta tgttaatatt ttttcagcaa
     9901 gaaaacgtaa attacatgag ttgaaaaata tgtattaaat atataataaa ataaccacat
     9961 gttttacata aacacggtgg ttattttatc aatttttata ttgccttttt tctgaatatt
    10021 aaaagactta tacctaatat tagtagtcca gaagcgccaa ttattgacaa tatatcactt
    10081 tggttgcttt ctcctgtttc tggtaatact tgagagtttg aatcttgatt tttcattcct
    10141 tcagaactct tgtttttatt ttcagttaca ggtgtcaaca caccttgagg tgtattaata
    10201 gttttatttt cttcattagc tatcttttga ctttcatttt ttacaatttg attatatttt
    10261 tctgtggata cactattagg gttaggaaca tgactttcta attcttggta ttcttcttga
    10321 gtcatacaat taggtatcac ccctccgaaa ggtcttttac cttgttgagt tctctcatta
    10381 ttttgtatat cagtactttg tgattgatat tcattaaata cacttgaaac tttctcatta
    10441 tactcgattt gagttaattc ccctgaatct ttctgtttag aaagttcact tatttgattt
    10501 tgtgcagatt gtgggaggga tgaagtattg aagtctacat tagcccaacc atctccattt
    10561 gtatctgcgc tatttggtaa gttctctccg tatacggttt tggcaccatc ctcagcattg
    10621 gctgtcgctg aaaaacctgt gaatgttaat gagctagcca ataataaagt tggaagtaat
    10681 gatcgttttt tcataaaatt atctccttta atagaatttg tattttcatt atcacataga
    10741 tacattaaac ataaaatgac ttatcggaag tgcatatgct taacaattca caaatgatat
    10801 aatataaaag tcgtcgttct acttctgtgc tggtttagca tggaaggagg tgacaccatg
    10861 ttcagcatca tatttgtatc tatagtagct cctattatag taggggttat aatcacgttg
    10921 ttttcacatt ggttgaataa tcgtgataaa caataaagcg acgacacagt aataacacca
    10981 aaaagagccc gataactact ggaatagtta tcgggctcgg tgtttaccat gttcaacaca
    11041 tatttgttat ctaaattata acatcatagt gtggaggaat gcaaaattga ataattagta
    11101 tgttaagtct atactatact gattagtaag tactaattat catctttgtt attgttaccc
    11161 ctttcattgg cttttgaatc taattctttt tgaaattttt tgtgtgattt tgaagaagtg
    11221 tttaaaaaga aataggtcat attatccata cgatgtcctt ttataaattc attgataaat
    11281 tgtttttttg tcattttttt ggttgtatta cgttgttttt gcaatctaat gatttcttct
    11341 tcaatgtata ctcttatcat caaatctaaa ctagatgtct gattataagt agtgaataat
    11401 tttggaacta ataaagtttt attaccatca aaaagaaatg gagtgaaatc ataagtttcc
    11461 catataccgt tagtagagaa ataataatat gtcacagttt ctttagaaag aaacgatttc
    11521 atttctggaa atgactgaat catggattta ttaaaatcaa ttataacatt catgattata
    11581 ttacttgtta aatctgataa tttatcatca ccaaaatttc tgattaatgg tgctttttga
    11641 ggtattgtta atccgtcatc atttaagata atggaaagaa tatcttcatt cataaataga
    11701 ttaatcaatt cagatgctgt agtacctttt cctgtattac ctttctgaga aaaacctaag
    11761 cgtaaatggt gtggttctga aaaatgagag aataattttc taaattcatt acgcttttgg
    11821 gtatcagaaa aatcactaag atgtatatcg ataatttttt caaaaaaaac atcaatttgc
    11881 tcttgtgctc tttgagccaa agtcgcaact tttccaactt ctctattaga tattacttta
    11941 ataaggtttg ggtctatata ttgaagagtg tcttcttcaa tagaaatatt taaataatca
    12001 gttgatttag catcatgatt gaatttggta catactagtg tcactctata tcatccttct
    12061 gtaagttttt tgtttacttc caatatagaa tgatgtatta ttgtaattgt cgatttacaa
    12121 taagttgtta ttctaggtgg aagtttattg cttttgatgt atagatagac ttaacctttt
    12181 cattatagca tactatagaa gaaaatgcaa gcaaaagcat ataaacaaca aatgttgttt
    12241 atatgctttt gcttttttgt taattggaga ggacaaggtg aatattaaca gtcaaaatcc
    12301 agttatgtcg gttaatgaat cgatgaaaat tttcaaagct caagtaatgc ttagaaattt
    12361 aaagctatta agcgagtgga ttgttaaagg ggactgaagt taaagacact ttttaagaag
    12421 atttttcaat taaagggtat attaaaactt taatttagta ctttatattt ttcttgactc
    12481 tttaataatc ctatatacag tagaacgact cactcctgtt tgttcagcaa tttcttcacc
    12541 agacagcttt tgttcatcat ataagaattt gatctctctt ttcttatgat ctggtagtga
    12601 aggtcttcca ccttttctcc ctcttgctct tgcagcttcc aaaccttttt tagttcgttc
    12661 acttaataaa ttagcttcaa gttcagcgaa tgcactcatc atggtaaaga acatcttgcc
    12721 catcgcatct ttcgttgaca cattcatatc tataatatgt aaatcaatgc tattttcatc
    12781 caaccattga gatagctcaa ttaattgttt tgttgtacgt ccaagacgat ctaatttata
    12841 gacaactaga atatctcctt cacgtaaata atctaaacac ttatcaagtt ccgttctttt
    12901 agttttacga ccactcgctt tctcgctaaa gatacgttca caaccatatt cctcaagtgt
    12961 atcaatctgt ccatcaagac tttgatcttg tgttgacaca cgtgcataac caattttagc
    13021 catttgaaca ccaccttttt taatcgtttt atgggtactt actaatgaaa tatagttctc
    13081 gatgtaaata aacacgtctg tcagcatcta aaaactcatg attttcgata tttatgatac
    13141 attgattata acacaagttt atagacttaa atatccaaga aaaacctctt aatattaagt
    13201 taagaggttt tgtgtcaaaa acgaacgtaa tttagaaata atatttattg gtatttcaag
    13261 aatatatata taattattta ctaagtccat ttaaaaaaat ttgagagaac ttgttcataa
    13321 gtttaattct ttcattaata ttttgttcat gtgtaaaggt aacaattcca ttaacagcgt
    13381 ttgctgctat tttactaaca gcattaacat cattaataca ccattcgcca tttaaattac
    13441 cctccttaaa aattacatga taagcatcta tatatttgtt ttctaattta ttcatttttt
    13501 cattaatgct attagtttta taatattcag tgtaaaattc aattattgcg ttttgaagtg
    13561 gataatagta
//
