LOCUS       NZ_CP073896            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain V1937538 plasmid p2V1937538,
            complete sequence.
ACCESSION   NZ_CP073896
VERSION     NZ_CP073896.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699898
            Assembly: GCF_019330185.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 394x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019330185.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 07:01:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,701
            CDSs (total)                      :: 2,620
            Genes (coding)                    :: 2,542
            CDSs (with protein)               :: 2,542
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 40 of 78
            Pseudo Genes (incomplete)         :: 43 of 78
            Pseudo Genes (internal stop)      :: 28 of 78
            Pseudo Genes (multiple problems)  :: 24 of 78
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073896.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="V1937538"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p2V1937538"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(13417..13570,1..86))
                     /locus_tag="KFV38_RS13180"
                     /old_locus_tag="KFV38_13190"
     CDS             complement(join(13417..13570,1..86))
                     /locus_tag="KFV38_RS13180"
                     /old_locus_tag="KFV38_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(103..372)
                     /locus_tag="KFV38_RS13185"
                     /old_locus_tag="KFV38_13195"
     CDS             complement(103..372)
                     /locus_tag="KFV38_RS13185"
                     /old_locus_tag="KFV38_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            581..2503
                     /locus_tag="KFV38_RS13190"
                     /old_locus_tag="KFV38_13200"
     CDS             581..2503
                     /locus_tag="KFV38_RS13190"
                     /old_locus_tag="KFV38_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            2777..3190
                     /locus_tag="KFV38_RS13615"
                     /old_locus_tag="KFV38_13205"
     CDS             2777..3190
                     /locus_tag="KFV38_RS13615"
                     /old_locus_tag="KFV38_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            3267..3500
                     /locus_tag="KFV38_RS13200"
                     /old_locus_tag="KFV38_13210"
     CDS             3267..3500
                     /locus_tag="KFV38_RS13200"
                     /old_locus_tag="KFV38_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(3549..4253)
                     /locus_tag="KFV38_RS13205"
                     /old_locus_tag="KFV38_13215"
     CDS             complement(3549..4253)
                     /locus_tag="KFV38_RS13205"
                     /old_locus_tag="KFV38_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(4404..5102)
                     /locus_tag="KFV38_RS13210"
                     /old_locus_tag="KFV38_13220"
     CDS             complement(4404..5102)
                     /locus_tag="KFV38_RS13210"
                     /old_locus_tag="KFV38_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            5266..5364
                     /locus_tag="KFV38_RS13215"
                     /old_locus_tag="KFV38_13225"
     CDS             5266..5364
                     /locus_tag="KFV38_RS13215"
                     /old_locus_tag="KFV38_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5541..6452)
                     /locus_tag="KFV38_RS13220"
                     /old_locus_tag="KFV38_13230"
     CDS             complement(5541..6452)
                     /locus_tag="KFV38_RS13220"
                     /old_locus_tag="KFV38_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(6871..7431)
                     /locus_tag="KFV38_RS13225"
                     /old_locus_tag="KFV38_13235"
     CDS             complement(6871..7431)
                     /locus_tag="KFV38_RS13225"
                     /old_locus_tag="KFV38_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            complement(7682..8248)
                     /gene="qacR"
                     /locus_tag="KFV38_RS13230"
                     /old_locus_tag="KFV38_13240"
     ARG             complement(7686..8248)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            8427..9971
                     /gene="qac"
                     /locus_tag="KFV38_RS13235"
                     /old_locus_tag="KFV38_13245"
     CDS             8427..9971
                     /gene="qac"
                     /locus_tag="KFV38_RS13235"
                     /old_locus_tag="KFV38_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            10176..10736
                     /locus_tag="KFV38_RS13240"
                     /old_locus_tag="KFV38_13250"
     CDS             10176..10736
                     /locus_tag="KFV38_RS13240"
                     /old_locus_tag="KFV38_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(11055..11816)
                     /locus_tag="KFV38_RS13250"
                     /old_locus_tag="KFV38_13260"
     CDS             complement(11055..11816)
                     /locus_tag="KFV38_RS13250"
                     /old_locus_tag="KFV38_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            12257..13192
                     /locus_tag="KFV38_RS13255"
                     /old_locus_tag="KFV38_13265"
     CDS             12257..13192
                     /locus_tag="KFV38_RS13255"
                     /old_locus_tag="KFV38_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     ARG             8428..9968
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          12258..13192
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          582..2434
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 ctatctaaat tttttaatct atgctttctt aattctgctt tttcaatttc agcatttatt
       61 tttttgagat gtttaaaatc attcatcgta tcacttacta ttctagttaa ttttgtcttc
      121 tgttaaattt tccttgttct cgttgctgct attttgttcg atatctttat taaattctcc
      181 aatgacttta tccataattt tatacaattc atcactagag ttgatcatat ttaaatcaag
      241 tttatttaaa aaataaccac caattttatt agatagttgt tcttcttgtt ttttcttttg
      301 ctctttcagt tgtgcaattt gtttttctaa ttgacttaat tttgtaatat ctgtttgttt
      361 aattttagac attaaaaaac tcccttagtt tatctatata aagcatacca taattcattg
      421 ggtaagtcgc acatccatgg tataatataa ccagttctta agacactgca ctgcccattt
      481 acatgcaaat tgaaaattgg cataaaaatg ggcaaccata ctggttgatc attatagttc
      541 ccacaggggt aaaaaaacag aaaagagagg aggtaaaatc atgaataaag gttactttat
      601 gttgagagtg actaacatta atcgagcaac aaaaaatatc gttgcgtcag cgtcttatcg
      661 ttctgatgaa gcattatata gcgaacgtac agatgaaaaa attaagttta gaaatcatac
      721 tattaagcct gaaagcatga ttttaactcc tcaacatgca cccgaatgga ctaaagatag
      781 acagcgttta tggaatgaag ttgataaagt tgaaaagaat aatgcgaaaa caaaaaatcc
      841 tagattagca aaagaagtct tattaagttt gcctaatgat tttgatagag aggtacaaac
      901 tgaattaact aaagatttta taaaaagtga gtttgtagac aaaggaatgg ttgctgatat
      961 ttcaattcat agagatgata taaacaaccc acacgcccat gttctactta ctcaaagacc
     1021 gtttaatgca gacggtactt gggggaaaaa gacaaaaacg agaacgcaat atgatgaaaa
     1081 tggcaatgcg attttaaata aaaatggtaa taaagttaga aaacaagaac gatttgccga
     1141 ctttgatttc aaagaagttc gtaagcattg ggaattaaaa ttaaatcaat actcagaacg
     1201 tgaacaaagt ttaagacgat atgatagtcg ttcttttgaa gcgcaaggtt tagaaaaaat
     1261 tgccgaaatt ccacttacaa gagaagaata tagattagaa caaaatgaaa gaaaacgctg
     1321 tgaaaaagag ggtatcgagt accaacccgt tacttattac ggacagaaaa atgaacaaat
     1381 tagaagatat aatcgtggcg aagtatcaca attatttagt gataaagaga aagaacaagc
     1441 acaaaatgaa attagattat ttattcaaaa tgctaacgaa caagtaagta aaaatgaaca
     1501 aagttattct attttatttg atagatatca acgtgatgtt ggctattcag aggcgaaaga
     1561 aacaattaaa aatacgtttg acagtgcgtc tacctttggt cgtaaaattc aaaatgattt
     1621 acttaaaaat gaaattaaga gacgtcaatt aaaattctat gctaaaaatt atgatgaaaa
     1681 ttatcagttt agaaaagaaa ttgaaagtaa aggatatagt caacaagagt ttgttaatac
     1741 tgttgaaact aactataaag cgttactcaa tgagaagaat aaattagaaa gtagagagga
     1801 aaaacgtaaa gagatttatc aatcagctaa agatgtttat ctaactgaaa gtgaaaaaaa
     1861 tcatctcatt gttcagcaaa tgtaccctaa atctcatcat cattttagcc atgatgaaaa
     1921 agcatttatt gttgatgagg cacaaaaagg taatattata aaagcaactc atgttcaata
     1981 cctgttcaaa caaaataaca atattcctaa tgatgtattg ctagaagata cttataaaaa
     2041 agcgagtaaa gatattttct ttagtcaaaa acatcttaaa aatttagaaa aagatagctt
     2101 tgaatataac gttgagttgc aatttattaa ggaaaaagaa aaagaaattg atagttataa
     2161 agaacatatt gatgatgatt taagaataga tataggcgat aaaaactttg aaaaagtcaa
     2221 agaccaaaca cctaatgaaa aagttcaatt actcattaaa cttgagaaat ataatcatgt
     2281 cgatcaagaa gaagttatcg aaaaacatat taatcaagta gatagaaatg atcatgacaa
     2341 tcgtattgaa catgaaaatg aacaaactaa tcacattaat ttaccaacga taaatgctag
     2401 tgactttctt atgtactatg cgaacgaaac tgaaaagcaa aacaatatta atcatcagaa
     2461 aaagaagaag aaacaagaga gaaataacca aatgagaaga tagtaagcag ttgaaatcat
     2521 acaaagttca ttctttaata ataagaaact taacccaatg tggtaaatta gctttggttt
     2581 aaagaaaaca tttatatact tagataaggt acattctatg tattatcaca atgtatttta
     2641 aaacgtgtat aattatagaa aaaccttgat attatgcgat tgttcacata tttcagcata
     2701 aaaaatacta atgttcataa atatcgatag aaaaaattga gtttaaatat atcttaaagt
     2761 taaggagaaa aataatttgg aaacaaaaat tggacatcaa atacaaaagt taagaaaaag
     2821 taaaaacatg tctcaagaaa aattagctga aaagttaggg gtatcgagac attctatttc
     2881 taattgggaa agagaagtaa gtaatcccga cttaaaaaca attttagaaa tcactaaatt
     2941 atttaatgtt tctctaaatc aattaattaa aggagtggaa atcatgcaag ttaataaata
     3001 tgtttactct gcattagcat tttttctagg aggtttcggg gctcataagt tttatgttaa
     3061 gaaaaataaa aatgctcttc tttatttact attttgttgg acgggaatac cgggtgtgat
     3121 aggtatggta gaaggtgttt taacacttat gcgtccgagc gatagtgaaa acaatataga
     3181 aataaattaa accaaaaata aaaagccgat gtatcggatt ttttttagtg tacatgtgaa
     3241 tattaaaaat gatggagtga tttcacatga acacattcgt tttcggcgtg attacaaagc
     3301 gattagaaga aataggtttt aatgtacaca aggtgtttaa aatgcccgaa aaacaggcac
     3361 tatttgaaga tttgtaccat tatgtgagat acgttaaacc atgcaaggct gaactaacaa
     3421 tggtggcatt agatagtgat gaattgattc attactttat atgctgttac atggaacgtt
     3481 atgcttatct tttgaactaa tgataaaaaa gacgcctaat tttaggcgcc tttttaataa
     3541 tctactaact acttattata tctaatttta cttgctaact tagctactgt tttattgtcg
     3601 tagggtttaa tatagactgt ttcaatattc tcctctattg ctttttttgt tatcttattc
     3661 atttgactat aatcactatt ttgtgaattt tcccatttat aagggatatt attatataca
     3721 ttaattgttc catcaccatt actgctatat gttacagacc caccagctct cataggagct
     3781 tctaatttca caactttttc tttataaatt gcactatttt tggcttgtgg atttactttt
     3841 gagccttttg gtatttctgt aacataaaca tttttaatac cttttaaatc atttcttgta
     3901 gatattaact gataccaaac tcgtgcatat tcaatttctt tcgagttaaa attatttaag
     3961 taacttttat ttttatcagt agattgttgc ttcttattac tatcattatt tttttcttgt
     4021 tttttattat catttttttc tttattttgt ttattactac tttctgaatt atccgcacta
     4081 tcagatgtgt tagttttttc gtcaatttca tttgatgctt cattatcttt gttttctgag
     4141 gtgttagtat cactagaatt attatcattg tgatgttctg aatcgttatt attacaactc
     4201 actaaaatta ccgataaact aacaattaat ataggtaaaa tgattttttt catattacaa
     4261 ctccttttca gtatttcaaa ttagattatg ttaatatttt ttcagcaaga aaacgtaaat
     4321 tacatgagtt gaaaaatatg tattaaatat ataataaaat aaccacatgt tttacataaa
     4381 cacggtggtt attttatcaa tttttatatt gccttttttc tgaatattaa aagacttata
     4441 cctaatatta gtagtccaga agcgccaatt attgacaata tatcactttg gttgctttct
     4501 cctgtttctg gtaatacttg agagtttgaa tcttgatttt tcattccttc agaactcttg
     4561 tttttatttt cagttacagg tgtcaacaca ccttgaggtg tattaatagt tttattttct
     4621 tcattagcta tcttttgact ttcatttttt acaatttgat tatatttttc tgtggataca
     4681 ctattagggt taggaacatg actttctaat tcttggtatt cttcttgagt catacaatta
     4741 ggtatcaccc ctccgaaagg tcttttacct tgttgagttc tctcattatt ttgtatatca
     4801 gtactttgtg attgatattc attaaataca cttgaaactt tctcattata ctcgatttga
     4861 gttaattccc ctgaatcttt ctgtttagaa agttcactta tttgattttg tgcagattgt
     4921 gggagggatg aagtattgaa gtctacatta gcccaaccat ctccatttgt atctgcgcta
     4981 tttggtaagt tctctccgta tacggttttg gcaccatcct cagcattggc tgtcgctgaa
     5041 aaacctgtga atgttaatga gctagccaat aataaagttg gaagtaatga tcgttttttc
     5101 ataaaattat ctcctttaat agaatttgta ttttcattat cacatagata cattaaacat
     5161 aaaatgactt atcggaagtg catatgctta acaattcaca aatgatataa tataaaagtc
     5221 gtcgttctac ttctgtgctg gtttagcatg gaaggaggtg acaccatgtt cagcatcata
     5281 tttgtatcta tagtagctcc tattatagta ggggttataa tcacgttgtt ttcacattgg
     5341 ttgaataatc gtgataaaca ataaagcgac gacacagtaa taacaccaaa aagagcccga
     5401 taactactgg aatagttatc gggctcggtg tttaccatgt tcaacacata tttgttatct
     5461 aaattataac atcatagtgt ggaggaatgc aaaattgaat aattagtatg ttaagtctat
     5521 actatactga ttagtaagta ctaattatca tctttgttat tgttacccct ttcattggct
     5581 tttgaatcta attctttttg aaattttttg tgtgattttg aagaagtgtt taaaaagaaa
     5641 taggtcatat tatccatacg atgtcctttt ataaattcat tgataaattg tttttttgtc
     5701 atttttttgg ttgtattacg ttgtttttgc aatctaatga tttcttcttc aatgtatact
     5761 cttatcatca aatctaaact agatgtctga ttataagtag tgaataattt tggaactaat
     5821 aaagttttat taccatcaaa aagaaatgga gtgaaatcat aagtttccca tataccgtta
     5881 gtagagaaat aataatatgt cacagtttct ttagaaagaa acgatttcat ttctggaaat
     5941 gactgaatca tggatttatt aaaatcaatt ataacattca tgattatatt acttgttaaa
     6001 tctgataatt tatcatcacc aaaatttctg attaatggtg ctttttgagg tattgttaat
     6061 ccgtcatcat ttaagataat ggaaagaata tcttcattca taaatagatt aatcaattca
     6121 gatgctgtag taccttttcc tgtattacct ttctgagaaa aacctaagcg taaatggtgt
     6181 ggttctgaaa aatgagagaa taattttcta aattcattac gcttttgggt atcagaaaaa
     6241 tcactaagat gtatatcgat aattttttca aaaaaaacat caatttgctc ttgtgctctt
     6301 tgagccaaag tcgcaacttt tccaacttct ctattagata ttactttaat aaggtttggg
     6361 tctatatatt gaagagtgtc ttcttcaata gaaatattta aataatcagt tgatttagca
     6421 tcatgattga atttggtaca tactagtgtc actctatatc atccttctgt aagttttttg
     6481 tttacttcca atatagaatg atgtattatt gtaattgtcg atttacaata agttgttatt
     6541 ctaggtggaa gtttattgct tttgatgtat agatagactt aaccttttca ttatagcata
     6601 ctatagaaga aaatgcaagc aaaagcatat aaacaacaaa tgttgtttat atgcttttgc
     6661 ttttttgtta attggagagg acaaggtgaa tattaacagt caaaatccag ttatgtcggt
     6721 taatgaatcg atgaaaattt tcaaagctca agtaatgctt agaaatttaa agctattaag
     6781 cgagtggatt gttaaagggg actgaagtta aagacacttt ttaagaagat ttttcaatta
     6841 aagggtatat taaaacttta atttagtact ttatattttt cttgactctt taataatcct
     6901 atatacagta gaacgactca ctcctgtttg ttcagcaatt tcttcaccag acagcttttg
     6961 ttcatcatat aagaatttga tctctctttt cttatgatct ggtagtgaag gtcttccacc
     7021 ttttctccct cttgctcttg cagcttccaa acctttttta gttcgttcac ttaataaatt
     7081 agcttcaagt tcagcgaatg cactcatcat ggtaaagaac atcttgccca tcgcatcttt
     7141 cgttgacaca ttcatatcta taatatgtaa atcaatgcta ttttcatcca accattgaga
     7201 tagctcaatt aattgttttg ttgtacgtcc aagacgatct aatttataga caactagaat
     7261 atctccttca cgtaaataat ctaaacactt atcaagttcc gttcttttag ttttacgacc
     7321 actcgctttc tcgctaaaga tacgttcaca accatattcc tcaagtgtat caatctgtcc
     7381 atcaagactt tgatcttgtg ttgacacacg tgcataacca attttagcca tttgaacacc
     7441 acctttttta atcgttttat gggtacttac taatgaaata tagttctcga tgtaaataaa
     7501 cacgtctgtc agcatctaaa aactcatgat tttcgatatt tatgatacat tgattataac
     7561 acaagtttat agacttaaat atccaagaaa aacctcttaa tattaagtta agaggttttg
     7621 tgtcaaaaac gaacgtaatt tagaaataat atttattggt atttcaagaa tatatatata
     7681 attatttact aagtccattt aaaaaaattt gagagaactt gttcataagt ttaattcttt
     7741 cattaatatt ttgttcatgt gtaaaggtaa caattccatt aacagcgttt gctgctattt
     7801 tactaacagc attaacatca ttaatacacc attcgccatt taaattaccc tccttaaaaa
     7861 ttacatgata agcatctata tatttgtttt ctaatttatt cattttttca ttaatgctat
     7921 tagttttata atattcagtg taaaattcaa ttattgcgtt ttgaagtgga taatagtatt
     7981 cggttgttaa agatagttca ttatataaat aaaatttttc tctattagtt ttacatttga
     8041 tttgttcctt tttccactgt tcttgccatt tagattcttc tatatttaaa atttctaaaa
     8101 atagattttc ttttgtttta aagtgataat aaagattccc tttactactt tctgataatt
     8161 taacaatttc tccagtagta gtggcattat atccattttt tataaataat tcctttgcga
     8221 cacctagtat tttatctttc aagttcatca cgcctttaat gagtattaag tactatcaat
     8281 gatagcacaa aaaattcaaa gaataattgt ataacacaat acagccattt aaatttcgca
     8341 agattttttg ttgtaatatg taaaaaaaat agattataat ccttatagac cgatcgcacg
     8401 gtctataagg attggaggga acttaaatga tttcattttt tacaaaaact actgatatga
     8461 tgacatcaaa aaaaagatgg actgcactag tagtattagc tgttagtttg tttgttgtta
     8521 caatggatat gacaatatta attatggctt taccggaatt agtaagagag ttagagcctt
     8581 ctggtaccca acagttatgg atagttgata tatactctct tgttttagct ggctttataa
     8641 ttccattgag tgcctttgct gataaatggg gaagaaaaaa agcattatta actggatttg
     8701 ctttatttgg cctcgtttca ttagctatat ttttcgcaga aagtgcagag ttcgtaatag
     8761 ctattcgatt tttacttggt attgcaggtg ctttaataat gccaactact ctttcaatga
     8821 taagagtaat ttttgaaaac cctaaagaaa gggccactgc attagctgta tggtcaatcg
     8881 cttcatcgat aggtgctgtt tttggaccaa ttatcggagg agctttactt gagcaatttt
     8941 catggcactc ggcattttta attaatgtac cgtttgcgat aatagcagtt gtagcaggtt
     9001 tatttttatt accagagtct aagttatcaa aagaaaagtc tcactcgtgg gatattcctt
     9061 ctacaatttt atcaattgca ggcatgattg gactggtatg gagtatcaaa gaattttcaa
     9121 aagaaggact agcagatatt attccatggg ttgtaatagt attagcaatt accatgatag
     9181 tgatatttgt taaacgtaat ttatcaagtt ctgatccaat gttagacgta agacttttta
     9241 aaaagagatc attttcagct ggtacaattg ctgcatttat gacaattttt gcaatggcat
     9301 ctgttttgtt attagcttca caatggttac aggttgtgga agaactttct ccttttaaag
     9361 ctggcttata cctattacct atggcaatag gagatatggt gtttgcacca attgcacccg
     9421 gattagcggc gcgatttgga ccgaaaatag tgttaccttc cggaattgga attgcagcca
     9481 ttggcatgtt tattatgtat ttctttggtc atccattatc atattctaca atggctttag
     9541 cattaatttt agttggagct ggtatggctt cactagcagt tgcatctgct ctaataatgt
     9601 tagaaacacc tacatcaaaa gcaggtaatg cagctgctgt tgaagagtct atgtatgacc
     9661 ttggaaatgt ttttggtgta gcagtacttg gtagcctatc ttctatgctt tatcgtgtat
     9721 ttttagatat ttcatctttt tcatcaaaag gtatagttgg agatttagct catgtagctg
     9781 aagaatctgt agtgggcgct gtcgaagtag ctaaagctac ggggataaaa cagcttgcaa
     9841 acgaggctgt aacatcattt aatgatgctt ttgtagcaac tgctttagta ggtgggatta
     9901 tcatgattat catttcaata gttgtctatt tgttaattcc caaatcactt gatataacta
     9961 aacaaaaata gtactaaaca tatattttta gatattgtag attagtttat ttttaaaatt
    10021 agtaatagtt aaaaagtaga accattaatt ttaaatggtt ctactttttt attattaagt
    10081 aaaattataa aagacaattt cttaaattaa taatatgata tacctatatc taataattta
    10141 ttagtattaa taaaccttga aaggatgatt aactgatgaa tagagcagat atttttaaaa
    10201 tatttgatgt agaacttaat ataactatat ttttaggagc actagtagca actttcatct
    10261 attttataag ttacatgaac aatgtaaatg aattaattac attatgctta acattagctt
    10321 ttcctgcata tttattaaaa attagcagat cgttcgaggt tattttatta gaggaaaaaa
    10381 taaaaaatag taaaatcaaa atctgtgaca aagatagaat tgaacgagag atactggtga
    10441 aaaagaacct aatagatgaa ctattgtttc ctaatagagt tattaaaaaa tatacactga
    10501 caattttaat ttacagtata ggtattatta ttcttttact actttgtaat atgttaagtt
    10561 atggaattgg tgaaaatgtc aattttatct ggagattact agcttgttat gtaacgagtg
    10621 tcacagtgat tatagttgcc ttgattataa tgataatgat atttgatctt ataattggat
    10681 tactattaaa ttttcaaata tttttaaaaa ataaaaagta tgatgatttt ttataattat
    10741 tcgatttata taatgaaaaa ttaaatgatt aaacatagaa acctcaaaac aaaccatatt
    10801 taatagagtt tatggcgcct tatacaaata aaggctatgc tgttaaagaa ttatgtagct
    10861 atcttcagat taatatggat gatgtttata ctattggcga tagaaaattg atttacctat
    10921 gtttaatacc ctagataatt cttctgataa tttagttttt gttagagttt aacataactg
    10981 atgattatca gaagtttgat tcacattaaa aaggcacaac aagttcatga acttgtgtgc
    11041 ctttttaatt gcaattagtt accaaataaa cgactccaaa atctcttttt aggtggttct
    11101 acctccctat tatcactgga atcgttcttc gttttttcaa caaacttatc ctcttttgtt
    11161 aattcatctg tactgtcatg cttattctct tcagttatat ttttatgatt tacttcggtt
    11221 ggttgacatt ctttttgttg ttggttgata ttttgagatt cttcagtaaa agttgcttct
    11281 tgtggattaa tattctgtct attattcatc gttgtatcaa aagaatattt aagctgtctt
    11341 tcttcttcta attggctttc tagtttttga atttttttat tgttttctaa agataatatt
    11401 tgttgattct ccaacagttt atttaatgta ttaacattgc tattttgttg atctaattgt
    11461 ttagttagat tctcaatata tttttcatca tttttagctc tagtttcaaa gatttctatt
    11521 tgcttttcta attcgtttac ttttgttttt aatgtctcag aactatttga attattatgt
    11581 atatactttt caacttttgc agtattttca ttaaataatt cattttttac tgattcttta
    11641 ttatgagttg ttttctcttt cttagtgatt ttttctataa tttttaaata atcattatct
    11701 ttaatataat taattccatt ttctttttca aaagatatat ttaatctttt agcattatta
    11761 acaatagttt gtttagttac acctaattca tcagcaatca ttttaatagt tttcataaca
    11821 tcccccctta ggtacctaat ttagtaccta aattttacca cctaacaaaa tttatttcat
    11881 catttagtac taaatttact atctattaac tacctaaatg cctaattatc tatccttaaa
    11941 atattttcgc cacttaatta ggtgctaaaa tagcgaatta tacgtttggt agttttaggt
    12001 gtacttttaa ttacatttaa aactctttat atacgccatt aaaagtgtta atattacttt
    12061 ataaatatta aaagagtcga tgctattggc gtagcatcga ctctcggtaa taaaacgatt
    12121 cgcactcgtt tgtttatata tttttttgat acttgtatta tatatatcta atcatctaag
    12181 tgcaagcaca aaacatataa cttacgtaaa aattgtttta ttacctcaat cccaaaatgg
    12241 aaatgaggtt tttattatgc ccaattttga aaaatataat ttatcacaag taaaaactga
    12301 aagattttat caactgccta aatatttatt tgaagatgca tattttaaga aaatgtctgc
    12361 agaagccaaa attatgtatg cgttattaaa agatcgtttt gaattatccc tccaaaatga
    12421 atgggtagat aaaaataata atatttactt tattttcagt aataaacatt tgtgtgaata
    12481 cttaggttat gcagaacaaa aaattataaa attaaaaaaa gagttaataa aatttaattt
    12541 actaactcaa gaacgtgttg gccttaataa accaaataga ttatacctat taaaacctaa
    12601 ttatgacatt gaagccagtc atatcaagga acttccaaat tcacagttcc agaacaatga
    12661 atttggaagt tctagaactg tgaatttaag tggtcaagaa cttccaaatt cacagtctaa
    12721 tgatactgat tataatgaca ctgattatat taagactaat tataatgata tgtatgattt
    12781 gaatgatagg aaattaacat attctagtaa tcatacaaat cattcaaatc actactattc
    12841 aaaattcaat gatgaagctt taaaatttca attacttgaa gaactaccac aaagtattca
    12901 aagttattta agtaactttt ctgtatctga aattaaactt attaaatctg tgttattaaa
    12961 agccaaaaca tccttcaaca attctattga tgcatattat ttattagaag atatggaatt
    13021 tgaaattgtt aatgttctta agcgttttaa agctacatta attcaaaaaa atgaaaccgt
    13081 tgaagcaatg caagggtact taatgaaatc tcttaaatct gaatttgcag aagtacatac
    13141 gcttaataaa cgacgtgatc atttaccaat cacttcttta tttaatcaat aatacaactt
    13201 aataagccaa caatgatcca attagagaat atctttaatt agatgttctc ttaactgtaa
    13261 gcgaacttta ttttcaatga aatgtaatta attaagtaag tgggactaaa acttttcttt
    13321 tttagtccca tttttataaa caattattat tcattgttac tactatcagc aattttttct
    13381 ttttagtgaa acccaagtat ttgtaagcat gataaattac ttctttttat atttattggg
    13441 tttatttgta ttaatgtagt tggaatacat ctttaacaat tcttctgttt catcaggact
    13501 taaatgctca cattcaaaat acttttcaag cattgcacct tttcgaatca gtctatgtgc
    13561 tctttctttt
//
