LOCUS       NZ_CP073892            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain V1936703 plasmid p1V1936703,
            complete sequence.
ACCESSION   NZ_CP073892
VERSION     NZ_CP073892.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699897
            Assembly: GCF_019330165.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 328x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019330165.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 07:05:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,602
            Genes (coding)                    :: 2,521
            CDSs (with protein)               :: 2,521
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 44 of 81
            Pseudo Genes (incomplete)         :: 43 of 81
            Pseudo Genes (internal stop)      :: 27 of 81
            Pseudo Genes (multiple problems)  :: 24 of 81
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073892.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="V1936703"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p1V1936703"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(12336..13570,1..310))
                     /gene="qac"
                     /locus_tag="KFV49_RS00005"
                     /old_locus_tag="KFV49_00005"
     CDS             complement(join(12336..13570,1..310))
                     /gene="qac"
                     /locus_tag="KFV49_RS00005"
                     /old_locus_tag="KFV49_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            489..1055
                     /gene="qacR"
                     /locus_tag="KFV49_RS00010"
                     /old_locus_tag="KFV49_00010"
     ARG             490..1052
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1306..1866
                     /locus_tag="KFV49_RS00015"
                     /old_locus_tag="KFV49_00015"
     CDS             1306..1866
                     /locus_tag="KFV49_RS00015"
                     /old_locus_tag="KFV49_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            2285..3196
                     /locus_tag="KFV49_RS00020"
                     /old_locus_tag="KFV49_00020"
     CDS             2285..3196
                     /locus_tag="KFV49_RS00020"
                     /old_locus_tag="KFV49_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(3373..3471)
                     /locus_tag="KFV49_RS00025"
                     /old_locus_tag="KFV49_00025"
     CDS             complement(3373..3471)
                     /locus_tag="KFV49_RS00025"
                     /old_locus_tag="KFV49_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3635..4333
                     /locus_tag="KFV49_RS00030"
                     /old_locus_tag="KFV49_00030"
     CDS             3635..4333
                     /locus_tag="KFV49_RS00030"
                     /old_locus_tag="KFV49_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            4484..5188
                     /locus_tag="KFV49_RS00035"
                     /old_locus_tag="KFV49_00035"
     CDS             4484..5188
                     /locus_tag="KFV49_RS00035"
                     /old_locus_tag="KFV49_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(5237..5470)
                     /locus_tag="KFV49_RS00040"
                     /old_locus_tag="KFV49_00040"
     CDS             complement(5237..5470)
                     /locus_tag="KFV49_RS00040"
                     /old_locus_tag="KFV49_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(5547..5960)
                     /locus_tag="KFV49_RS13495"
                     /old_locus_tag="KFV49_00045"
     CDS             complement(5547..5960)
                     /locus_tag="KFV49_RS13495"
                     /old_locus_tag="KFV49_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     gene            complement(6234..8156)
                     /locus_tag="KFV49_RS00050"
                     /old_locus_tag="KFV49_00050"
     CDS             complement(6234..8156)
                     /locus_tag="KFV49_RS00050"
                     /old_locus_tag="KFV49_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            8365..8634
                     /locus_tag="KFV49_RS00055"
                     /old_locus_tag="KFV49_00055"
     CDS             8365..8634
                     /locus_tag="KFV49_RS00055"
                     /old_locus_tag="KFV49_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            8651..8890
                     /locus_tag="KFV49_RS00060"
                     /old_locus_tag="KFV49_00060"
     CDS             8651..8890
                     /locus_tag="KFV49_RS00060"
                     /old_locus_tag="KFV49_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(9115..10050)
                     /locus_tag="KFV49_RS00065"
                     /old_locus_tag="KFV49_00065"
     CDS             complement(9115..10050)
                     /locus_tag="KFV49_RS00065"
                     /old_locus_tag="KFV49_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            10491..11252
                     /locus_tag="KFV49_RS00070"
                     /old_locus_tag="KFV49_00070"
     CDS             10491..11252
                     /locus_tag="KFV49_RS00070"
                     /old_locus_tag="KFV49_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(11571..12131)
                     /locus_tag="KFV49_RS00080"
                     /old_locus_tag="KFV49_00080"
     CDS             complement(11571..12131)
                     /locus_tag="KFV49_RS00080"
                     /old_locus_tag="KFV49_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     ARG             complement(12337..13568)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="99.76"
                     /coverage="79.77"
     ARG             complement(3..310)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="99.03"
                     /coverage="20.04"
     Typing          complement(9116..10050)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6304..8156)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 cgaaaaatat agctaatgaa acgaggccaa ataaagcaaa tccagttaat aatgcttttt
       61 ttcttcccca tttatcagca aaggcactca atggaattat aaagccagct aaaacaagag
      121 agtatatatc aactatccat aactgttggg taccagaagg ctctaactct cttactaatt
      181 ccggtaaagc cataattaat attgtcatat ccattgtaac aacaaacaaa ctaacagcta
      241 atactactag tgcagtccat cttttttttg atgtcatcat atcagtagtt tttgtaaaaa
      301 atgaaatcat ttaagttccc tccaatcctt atagaccgtg cgatcggtct ataaggatta
      361 taatctattt tttttacata ttacaacaaa aaatcttgcg aaatttaaat ggctgtattg
      421 tgttatacaa ttattctttg aattttttgt gctatcattg atagtactta atactcatta
      481 aaggcgtgat gaacttgaaa gataaaatac taggtgtcgc aaaggaatta tttataaaaa
      541 atggatataa tgccactact actggagaaa ttgttaaatt atcagaaagt agtaaaggga
      601 atctttatta tcactttaaa acaaaagaaa atctattttt agaaatttta aatatagaag
      661 aatctaaatg gcaagaacag tggaaaaagg aacaaatcaa atgtaaaact aatagagaaa
      721 aattttattt atataatgaa ctatctttaa caaccgaata ctattatcca cttcaaaacg
      781 caataattga attttacact gaatattata aaactaatag cattaatgaa aaaatgaata
      841 aattagaaaa caaatatata gatgcttatc atgtaatttt taaggagggt aatttaaatg
      901 gcgaatggtg tattaatgat gttaatgctg ttagtaaaat agcagcaaac gctgttaatg
      961 gaattgttac ctttacacat gaacaaaata ttaatgaaag aattaaactt atgaacaagt
     1021 tctctcaaat ttttttaaat ggacttagta aataattata tatatattct tgaaatacca
     1081 ataaatatta tttctaaatt acgttcgttt ttgacacaaa acctcttaac ttaatattaa
     1141 gaggtttttc ttggatattt aagtctataa acttgtgtta taatcaatgt atcataaata
     1201 tcgaaaatca tgagttttta gatgctgaca gacgtgttta tttacatcga gaactatatt
     1261 tcattagtaa gtacccataa aacgattaaa aaaggtggtg ttcaaatggc taaaattggt
     1321 tatgcacgtg tgtcaacaca agatcaaagt cttgatggac agattgatac acttgaggaa
     1381 tatggttgtg aacgtatctt tagcgagaaa gcgagtggtc gtaaaactaa aagaacggaa
     1441 cttgataagt gtttagatta tttacgtgaa ggagatattc tagttgtcta taaattagat
     1501 cgtcttggac gtacaacaaa acaattaatt gagctatctc aatggttgga tgaaaatagc
     1561 attgatttac atattataga tatgaatgtg tcaacgaaag atgcgatggg caagatgttc
     1621 tttaccatga tgagtgcatt cgctgaactt gaagctaatt tattaagtga acgaactaaa
     1681 aaaggtttgg aagctgcaag agcaagaggg agaaaaggtg gaagaccttc actaccagat
     1741 cataagaaaa gagagatcaa attcttatat gatgaacaaa agctgtctgg tgaagaaatt
     1801 gctgaacaaa caggagtgag tcgttctact gtatatagga ttattaaaga gtcaagaaaa
     1861 atataaagta ctaaattaaa gttttaatat accctttaat tgaaaaatct tcttaaaaag
     1921 tgtctttaac ttcagtcccc tttaacaatc cactcgctta atagctttaa atttctaagc
     1981 attacttgag ctttgaaaat tttcatcgat tcattaaccg acataactgg attttgactg
     2041 ttaatattca ccttgtcctc tccaattaac aaaaaagcaa aagcatataa acaacatttg
     2101 ttgtttatat gcttttgctt gcattttctt ctatagtatg ctataatgaa aaggttaagt
     2161 ctatctatac atcaaaagca ataaacttcc acctagaata acaacttatt gtaaatcgac
     2221 aattacaata atacatcatt ctatattgga agtaaacaaa aaacttacag aaggatgata
     2281 tagagtgaca ctagtatgta ccaaattcaa tcatgatgct aaatcaactg attatttaaa
     2341 tatttctatt gaagaagaca ctcttcaata tatagaccca aaccttatta aagtaatatc
     2401 taatagagaa gttggaaaag ttgcgacttt ggctcaaaga gcacaagagc aaattgatgt
     2461 tttttttgaa aaaattatcg atatacatct tagtgatttt tctgataccc aaaagcgtaa
     2521 tgaatttaga aaattattct ctcatttttc agaaccacac catttacgct taggtttttc
     2581 tcagaaaggt aatacaggaa aaggtactac agcatctgaa ttgattaatc tatttatgaa
     2641 tgaagatatt ctttccatta tcttaaatga tgacggatta acaatacctc aaaaagcacc
     2701 attaatcaga aattttggtg atgataaatt atcagattta acaagtaata taatcatgaa
     2761 tgttataatt gattttaata aatccatgat tcagtcattt ccagaaatga aatcgtttct
     2821 ttctaaagaa actgtgacat attattattt ctctactaac ggtatatggg aaacttatga
     2881 tttcactcca tttctttttg atggtaataa aactttatta gttccaaaat tattcactac
     2941 ttataatcag acatctagtt tagatttgat gataagagta tacattgaag aagaaatcat
     3001 tagattgcaa aaacaacgta atacaaccaa aaaaatgaca aaaaaacaat ttatcaatga
     3061 atttataaaa ggacatcgta tggataatat gacctatttc tttttaaaca cttcttcaaa
     3121 atcacacaaa aaatttcaaa aagaattaga ttcaaaagcc aatgaaaggg gtaacaataa
     3181 caaagatgat aattagtact tactaatcag tatagtatag acttaacata ctaattattc
     3241 aattttgcat tcctccacac tatgatgtta taatttagat aacaaatatg tgttgaacat
     3301 ggtaaacacc gagcccgata actattccag tagttatcgg gctctttttg gtgttattac
     3361 tgtgtcgtcg ctttattgtt tatcacgatt attcaaccaa tgtgaaaaca acgtgattat
     3421 aacccctact ataataggag ctactataga tacaaatatg atgctgaaca tggtgtcacc
     3481 tccttccatg ctaaaccagc acagaagtag aacgacgact tttatattat atcatttgtg
     3541 aattgttaag catatgcact tccgataagt cattttatgt ttaatgtatc tatgtgataa
     3601 tgaaaataca aattctatta aaggagataa ttttatgaaa aaacgatcat tacttccaac
     3661 tttattattg gctagctcat taacattcac aggtttttca gcgacagcca atgctgagga
     3721 tggtgccaaa accgtatacg gagagaactt accaaatagc gcagatacaa atggagatgg
     3781 ttgggctaat gtagacttca atacttcatc cctcccacaa tctgcacaaa atcaaataag
     3841 tgaactttct aaacagaaag attcagggga attaactcaa atcgagtata atgagaaagt
     3901 ttcaagtgta tttaatgaat atcaatcaca aagtactgat atacaaaata atgagagaac
     3961 tcaacaaggt aaaagacctt tcggaggggt gatacctaat tgtatgactc aagaagaata
     4021 ccaagaatta gaaagtcatg ttcctaaccc taatagtgta tccacagaaa aatataatca
     4081 aattgtaaaa aatgaaagtc aaaagatagc taatgaagaa aataaaacta ttaatacacc
     4141 tcaaggtgtg ttgacacctg taactgaaaa taaaaacaag agttctgaag gaatgaaaaa
     4201 tcaagattca aactctcaag tattaccaga aacaggagaa agcaaccaaa gtgatatatt
     4261 gtcaataatt ggcgcttctg gactactaat attaggtata agtcttttaa tattcagaaa
     4321 aaaggcaata taaaaattga taaaataacc accgtgttta tgtaaaacat gtggttattt
     4381 tattatatat ttaatacata tttttcaact catgtaattt acgttttctt gctgaaaaaa
     4441 tattaacata atctaatttg aaatactgaa aaggagttgt aatatgaaaa aaatcatttt
     4501 acctatatta attgttagtt tatcggtaat tttagtgagt tgtaataata acgattcaga
     4561 acatcacaat gataataatt ctagtgatac taacacctca gaaaacaaag ataatgaagc
     4621 atcaaatgaa attgacgaaa aaactaacac atctgatagt gcggataatt cagaaagtag
     4681 taataaacaa aataaagaaa aaaatgataa taaaaaacaa gaaaaaaata atgatagtaa
     4741 taagaagcaa caatctactg ataaaaataa aagttactta aataatttta actcgaaaga
     4801 aattgaatat gcacgagttt ggtatcagtt aatatctaca agaaatgatt taaaaggtat
     4861 taaaaatgtt tatgttacag aaataccaaa aggctcaaaa gtaaatccac aagccaaaaa
     4921 tagtgcaatt tataaagaaa aagttgtgaa attagaagct cctatgagag ctggtgggtc
     4981 tgtaacatat agcagtaatg gtgatggaac aattaatgta tataataata tcccttataa
     5041 atgggaaaat tcacaaaata gtgattatag tcaaatgaat aagataacaa aaaaagcaat
     5101 agaggagaat attgaaacag tctatattaa accctacgac aataaaacag tagctaagtt
     5161 agcaagtaaa attagatata ataagtagtt agtagattat taaaaaggcg cctaaaatta
     5221 ggcgtctttt ttatcattag ttcaaaagat aagcataacg ttccatgtaa cagcatataa
     5281 agtaatgaat caattcatca ctatctaatg ccaccattgt tagttcagcc ttgcatggtt
     5341 taacgtatct cacataatgg tacaaatctt caaatagtgc ctgtttttcg ggcattttaa
     5401 acaccttgtg tacattaaaa cctatttctt ctaatcgctt tgtaatcacg ccgaaaacga
     5461 atgtgttcat gtgaaatcac tccatcattt ttaatattca catgtacact aaaaaaaatc
     5521 cgatacatcg gctttttatt tttggtttaa tttatttcta tattgttttc actatcgctc
     5581 ggacgcataa gtgttaaaac accttctacc atacctatca cacccggtat tcccgtccaa
     5641 caaaatagta aataaagaag agcattttta tttttcttaa cataaaactt atgagccccg
     5701 aaacctccta gaaaaaatgc taatgcagag taaacatatt tattaacttg catgatttcc
     5761 actcctttaa ttaattgatt tagagaaaca ttaaataatt tagtgatttc taaaattgtt
     5821 tttaagtcgg gattacttac ttctctttcc caattagaaa tagaatgtct cgatacccct
     5881 aacttttcag ctaatttttc ttgagacatg tttttacttt ttcttaactt ttgtatttga
     5941 tgtccaattt ttgtttccaa attatttttc tccttaactt taagatatat ttaaactcaa
     6001 ttttttctat cgatatttat gaacattagt attttttatg ctgaaatatg tgaacaatcg
     6061 cataatatca aggtttttct ataattatac acgttttaaa atacattgtg ataatacata
     6121 gaatgtacct tatctaagta tataaatgtt ttctttaaac caaagctaat ttaccacatt
     6181 gggttaagtt tcttattatt aaagaatgaa ctttgtatga tttcaactgc ttactatctt
     6241 ctcatttggt tatttctctc ttgtttcttc ttctttttct gatgattaat attgttttgc
     6301 ttttcagttt cgttcgcata gtacataaga aagtcactag catttatcgt tggtaaatta
     6361 atgtgattag tttgttcatt ttcatgttca atacgattgt catgatcatt tctatctact
     6421 tgattaatat gtttttcgat aacttcttct tgatcgacat gattatattt ctcaagttta
     6481 atgagtaatt gaactttttc attaggtgtt tggtctttga ctttttcaaa gtttttatcg
     6541 cctatatcta ttcttaaatc atcatcaata tgttctttat aactatcaat ttctttttct
     6601 ttttccttaa taaattgcaa ctcaacgtta tattcaaagc tatctttttc taaattttta
     6661 agatgttttt gactaaagaa aatatcttta ctcgcttttt tataagtatc ttctagcaat
     6721 acatcattag gaatattgtt attttgtttg aacaggtatt gaacatgagt tgcttttata
     6781 atattacctt tttgtgcctc atcaacaata aatgcttttt catcatggct aaaatgatga
     6841 tgagatttag ggtacatttg ctgaacaatg agatgatttt tttcactttc agttagataa
     6901 acatctttag ctgattgata aatctcttta cgtttttcct ctctactttc taatttattc
     6961 ttctcattga gtaacgcttt atagttagtt tcaacagtat taacaaactc ttgttgacta
     7021 tatcctttac tttcaatttc ttttctaaac tgataatttt catcataatt tttagcatag
     7081 aattttaatt gacgtctctt aatttcattt ttaagtaaat cattttgaat tttacgacca
     7141 aaggtagacg cactgtcaaa cgtattttta attgtttctt tcgcctctga atagccaaca
     7201 tcacgttgat atctatcaaa taaaatagaa taactttgtt catttttact tacttgttcg
     7261 ttagcatttt gaataaataa tctaatttca ttttgtgctt gttctttctc tttatcacta
     7321 aataattgtg atacttcgcc acgattatat cttctaattt gttcattttt ctgtccgtaa
     7381 taagtaacgg gttggtactc gataccctct ttttcacagc gttttctttc attttgttct
     7441 aatctatatt cttctcttgt aagtggaatt tcggcaattt tttctaaacc ttgcgcttca
     7501 aaagaacgac tatcatatcg tcttaaactt tgttcacgtt ctgagtattg atttaatttt
     7561 aattcccaat gcttacgaac ttctttgaaa tcaaagtcgg caaatcgttc ttgttttcta
     7621 actttattac catttttatt taaaatcgca ttgccatttt catcatattg cgttctcgtt
     7681 tttgtctttt tcccccaagt accgtctgca ttaaacggtc tttgagtaag tagaacatgg
     7741 gcgtgtgggt tgtttatatc atctctatga attgaaatat cagcaaccat tcctttgtct
     7801 acaaactcac tttttataaa atctttagtt aattcagttt gtacctctct atcaaaatca
     7861 ttaggcaaac ttaataagac ttcttttgct aatctaggat tttttgtttt cgcattattc
     7921 ttttcaactt tatcaacttc attccataaa cgctgtctat ctttagtcca ttcgggtgca
     7981 tgttgaggag ttaaaatcat gctttcaggc ttaatagtat gatttctaaa cttaattttt
     8041 tcatctgtac gttcgctata taatgcttca tcagaacgat aagacgctga cgcaacgata
     8101 ttttttgttg ctcgattaat gttagtcact ctcaacataa agtaaccttt attcatgatt
     8161 ttacctcctc tcttttctgt ttttttaccc ctgtgggaac tataatgatc aaccagtatg
     8221 gttgcccatt tttatgccaa ttttcaattt gcatgtaaat gggcagtgca gtgtcttaag
     8281 aactggttat attataccat ggatgtgcga cttacccaat gaattatggt atgctttata
     8341 tagataaact aagggagttt tttaatgtct aaaattaaac aaacagatat tacaaaatta
     8401 agtcaattag aaaaacaaat tgcacaactg aaagagcaaa agaaaaaaca agaagaacaa
     8461 ctatctaata aaattggtgg ttatttttta aataaacttg atttaaatat gatcaactct
     8521 agtgatgaat tgtataaaat tatggataaa gtcattggag aatttaataa agatatcgaa
     8581 caaaatagca gcaacgagaa caaggaaaat ttaacagaag acaaaattaa ctagaatagt
     8641 aagtgatacg atgaatgatt ttaaacatct caaaaaaata aatgctgaaa ttgaaaaagc
     8701 agaattaaga aagcatagat taaaaaattt agatagaaaa gaaagagcac atagactgat
     8761 tcgaaaaggt gcaatgcttg aaaagtattt tgaatgtgag catttaagtc ctgatgaaac
     8821 agaagaattg ttaaagatgt attccaacta cattaataca aataaaccca ataaatataa
     8881 aaagaagtaa tttatcatgc ttacaaatac ttgggtttca ctaaaaagaa aaaattgctg
     8941 atagtagtaa caatgaataa taattgttta taaaaatggg actaaaaaag aaaagtttta
     9001 gtcccactta cttaattaat tacatttcat tgaaaataaa gttcgcttac agttaagaga
     9061 acatctaatt aaagatattc tctaattgga tcattgttgg cttattaagt tgtattattg
     9121 attaaataaa gaagtgattg gtaaatgatc acgtcgttta ttaagcgtat gtacttctgc
     9181 aaattcagat ttaagagatt tcattaagta cccttgcatt gcttcaacgg tttcattttt
     9241 ttgaattaat gtagctttaa aacgcttaag aacattaaca atttcaaatt ccatatcttc
     9301 taataaataa tatgcatcaa tagaattgtt gaaggatgtt ttggctttta ataacacaga
     9361 tttaataagt ttaatttcag atacagaaaa gttacttaaa taactttgaa tactttgtgg
     9421 tagttcttca agtaattgaa attttaaagc ttcatcattg aattttgaat agtagtgatt
     9481 tgaatgattt gtatgattac tagaatatgt taatttccta tcattcaaat catacatatc
     9541 attataatta gtcttaatat aatcagtgtc attataatca gtatcattag actgtgaatt
     9601 tggaagttct tgaccactta aattcacagt tctagaactt ccaaattcat tgttctggaa
     9661 ctgtgaattt ggaagttcct tgatatgact ggcttcaatg tcataattag gttttaatag
     9721 gtataatcta tttggtttat taaggccaac acgttcttga gttagtaaat taaattttat
     9781 taactctttt tttaatttta taattttttg ttctgcataa cctaagtatt cacacaaatg
     9841 tttattactg aaaataaagt aaatattatt atttttatct acccattcat tttggaggga
     9901 taattcaaaa cgatctttta ataacgcata cataattttg gcttctgcag acattttctt
     9961 aaaatatgca tcttcaaata aatatttagg cagttgataa aatctttcag tttttacttg
    10021 tgataaatta tatttttcaa aattgggcat aataaaaacc tcatttccat tttgggattg
    10081 aggtaataaa acaattttta cgtaagttat atgttttgtg cttgcactta gatgattaga
    10141 tatatataat acaagtatca aaaaaatata taaacaaacg agtgcgaatc gttttattac
    10201 cgagagtcga tgctacgcca atagcatcga ctcttttaat atttataaag taatattaac
    10261 acttttaatg gcgtatataa agagttttaa atgtaattaa aagtacacct aaaactacca
    10321 aacgtataat tcgctatttt agcacctaat taagtggcga aaatatttta aggatagata
    10381 attaggcatt taggtagtta atagatagta aatttagtac taaatgatga aataaatttt
    10441 gttaggtggt aaaatttagg tactaaatta ggtacctaag gggggatgtt atgaaaacta
    10501 ttaaaatgat tgctgatgaa ttaggtgtaa ctaaacaaac tattgttaat aatgctaaaa
    10561 gattaaatat atcttttgaa aaagaaaatg gaattaatta tattaaagat aatgattatt
    10621 taaaaattat agaaaaaatc actaagaaag agaaaacaac tcataataaa gaatcagtaa
    10681 aaaatgaatt atttaatgaa aatactgcaa aagttgaaaa gtatatacat aataattcaa
    10741 atagttctga gacattaaaa acaaaagtaa acgaattaga aaagcaaata gaaatctttg
    10801 aaactagagc taaaaatgat gaaaaatata ttgagaatct aactaaacaa ttagatcaac
    10861 aaaatagcaa tgttaataca ttaaataaac tgttggagaa tcaacaaata ttatctttag
    10921 aaaacaataa aaaaattcaa aaactagaaa gccaattaga agaagaaaga cagcttaaat
    10981 attcttttga tacaacgatg aataatagac agaatattaa tccacaagaa gcaactttta
    11041 ctgaagaatc tcaaaatatc aaccaacaac aaaaagaatg tcaaccaacc gaagtaaatc
    11101 ataaaaatat aactgaagag aataagcatg acagtacaga tgaattaaca aaagaggata
    11161 agtttgttga aaaaacgaag aacgattcca gtgataatag ggaggtagaa ccacctaaaa
    11221 agagattttg gagtcgttta tttggtaact aattgcaatt aaaaaggcac acaagttcat
    11281 gaacttgttg tgccttttta atgtgaatca aacttctgat aatcatcagt tatgttaaac
    11341 tctaacaaaa actaaattat cagaagaatt atctagggta ttaaacatag gtaaatcaat
    11401 tttctatcgc caatagtata aacatcatcc atattaatct gaagatagct acataattct
    11461 ttaacagcat agcctttatt tgtataaggc gccataaact ctattaaata tggtttgttt
    11521 tgaggtttct atgtttaatc atttaatttt tcattatata aatcgaataa ttataaaaaa
    11581 tcatcatact ttttattttt taaaaatatt tgaaaattta atagtaatcc aattataaga
    11641 tcaaatatca ttatcattat aatcaaggca actataatca ctgtgacact cgttacataa
    11701 caagctagta atctccagat aaaattgaca ttttcaccaa ttccataact taacatatta
    11761 caaagtagta aaagaataat aatacctata ctgtaaatta aaattgtcag tgtatatttt
    11821 ttaataactc tattaggaaa caatagttca tctattaggt tctttttcac cagtatctct
    11881 cgttcaattc tatctttgtc acagattttg attttactat tttttatttt ttcctctaat
    11941 aaaataacct cgaacgatct gctaattttt aataaatatg caggaaaagc taatgttaag
    12001 cataatgtaa ttaattcatt tacattgttc atgtaactta taaaatagat gaaagttgct
    12061 actagtgctc ctaaaaatat agttatatta agttctacat caaatatttt aaaaatatct
    12121 gctctattca tcagttaatc atcctttcaa ggtttattaa tactaataaa ttattagata
    12181 taggtatatc atattattaa tttaagaaat tgtcttttat aattttactt aataataaaa
    12241 aagtagaacc atttaaaatt aatggttcta ctttttaact attactaatt ttaaaaataa
    12301 actaatctac aatatctaaa aatatatgtt tagtactatt tttgtttagt tatatcaagt
    12361 gatttgggaa ttaacaaata gacaactatt gaaatgataa tcatgataat cccacctact
    12421 aaagcagttg ctacaaaagc atcattaaat gatgttacag cctcgtttgc aagctgtttt
    12481 atccccgtag ctttagctac ttcgacagcg cccactacag attcttcagc tacatgagct
    12541 aaatctccaa ctataccttt tgatgaaaaa gatgaaatat ctaaaaatac acgataaagc
    12601 atagaagata ggctaccaag tactgctaca ccaaaaacat ttccaaggtc atacatagac
    12661 tcttcaacag cagctgcatt acctgctttt gatgtaggtg tttctaacat tattagagca
    12721 gatgcaactg ctagtgaagc cataccagct ccaactaaaa ttaatgctaa agccattgta
    12781 gaatatgata atggatgacc aaagaaatac ataataaaca tgccaatggc tgcaattcca
    12841 attccggaag gtaacactat tttcggtcca aatcgcgccg ctaatccggg tgcaattggt
    12901 gcaaacacca tatctcctat tgccataggt aataggtata agccagcttt aaaaggagaa
    12961 agttcttcca caacctgtaa ccattgtgaa gctaataaca aaacagatgc cattgcaaaa
    13021 attgtcataa atgcagcaat tgtaccagct gaaaatgatc tctttttaaa aagtcttacg
    13081 tctaacattg gatcagaact tgataaatta cgtttaacaa atatcactat catggtaatt
    13141 gctaatacta ttacaaccca tggaataata tctgctagtc cttcttttga aaattctttg
    13201 atactccata ccagtccaat catgcctgca attgataaaa ttgtagaagg aatatcccac
    13261 gagtgagact tttcttttga taacttagac tctggtaata aaaataaacc tgctacaact
    13321 gctattatcg caaacggtac attaattaaa aatgccgagt gccatgaaaa ttgctcaagt
    13381 aaagctcctc cgataattgg tccaaaaaca gcacctatcg atgaagcgat tgaccataca
    13441 gctaatgcag tggccctttc tttagggttt tcaaaaatta ctcttatcat tgaaagagta
    13501 gttggcatta ttaaagcacc tgcaatacca agtaaaaatc gaatagctat tacgaactct
    13561 gcactttctg
//
