LOCUS       NZ_CP073889            13570 bp    DNA     circular CON 17-APR-2024
DEFINITION  Staphylococcus epidermidis strain V1933793 plasmid p2V1933793,
            complete sequence.
ACCESSION   NZ_CP073889
VERSION     NZ_CP073889.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18699896
            Assembly: GCF_019330145.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Papan,C., Schroder,M., Hoffmann,M., Knoll,H., Last,K., Albrecht,F.,
            Geisel,J., Fink,T., Gartner,B.C., Mellmann,A., Volk,T., Berger,F.K.
            and Becker,S.L.
  TITLE     Combined antibiotic stewardship and infection control measures to
            contain the spread of linezolid-resistant Staphylococcus epidermidis
            in an intensive care unit
  JOURNAL   Antimicrob Resist Infect Control 10 (1), 99 (2021)
   PUBMED   34193293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. SMRT Link V8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 355x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019330145.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 06:59:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,726
            CDSs (total)                      :: 2,645
            Genes (coding)                    :: 2,565
            CDSs (with protein)               :: 2,565
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 40 of 80
            Pseudo Genes (incomplete)         :: 45 of 80
            Pseudo Genes (internal stop)      :: 28 of 80
            Pseudo Genes (multiple problems)  :: 24 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP073889.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="V1933793"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p2V1933793"
                     /geo_loc_name="Germany"
                     /collected_by="Institute of Medical Microbiology and
                     Hygiene, Saarland University, Homburg, Germany"
     gene            complement(join(11789..13570,1..141))
                     /locus_tag="KFV31_RS00005"
                     /old_locus_tag="KFV31_00005"
     CDS             complement(join(11789..13570,1..141))
                     /locus_tag="KFV31_RS00005"
                     /old_locus_tag="KFV31_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001032622.1"
                     /translation="MNKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTIKPESMILTPQHAPEWTKDRQRLWNEVDKVEKNNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDINNPHAHVLLTQRPFNADGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNERKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANEQVSKNEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNTVETNYKALLNEK
                     NKLESREEKRKEIYQSAKDVYLTESEKNHLIVQQMYPKSHHHFSHDEKAFIVDEAQKGN
                     IIKATHVQYLFKQNNNIPNDVLLEDTYKKASKDIFFSQKHLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLRIDIGDKNFEKVKDQTPNEKVQLLIKLEKYNHVDQEEVIEKH
                     INQVDRNDHDNRIEHENEQTNHINLPTINASDFLMYYANETEKQNNINHQKKKKKQERN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            350..619
                     /locus_tag="KFV31_RS00010"
                     /old_locus_tag="KFV31_00010"
     CDS             350..619
                     /locus_tag="KFV31_RS00010"
                     /old_locus_tag="KFV31_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000034917.1"
                     /translation="MSKIKQTDITKLSQLEKQIAQLKEQKKKQEEQLSNKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIGEFNKDIEQNSSNENKENLTEDKIN"
                     /name="hypothetical protein"
     gene            636..875
                     /locus_tag="KFV31_RS00015"
                     /old_locus_tag="KFV31_00015"
     CDS             636..875
                     /locus_tag="KFV31_RS00015"
                     /old_locus_tag="KFV31_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_000997674.1"
                     /translation="MNDFKHLKKINAEIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSNYINTNKPNKYKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(1100..2035)
                     /locus_tag="KFV31_RS00020"
                     /old_locus_tag="KFV31_00020"
     CDS             complement(1100..2035)
                     /locus_tag="KFV31_RS00020"
                     /old_locus_tag="KFV31_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001132444.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNYNDMYDLNDRKLTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQSYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVNVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            2476..3237
                     /locus_tag="KFV31_RS00025"
                     /old_locus_tag="KFV31_00025"
     CDS             2476..3237
                     /locus_tag="KFV31_RS00025"
                     /old_locus_tag="KFV31_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000848986.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKEKTTHNKESVKNELFNENTAKVEKYIHNNSNSSETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLESQLEEERQLK
                     YSFDTTMNNRQNINPQEATFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKNDSSDNREVEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            complement(3556..4116)
                     /locus_tag="KFV31_RS00035"
                     /old_locus_tag="KFV31_00035"
     CDS             complement(3556..4116)
                     /locus_tag="KFV31_RS00035"
                     /old_locus_tag="KFV31_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(4321..5865)
                     /gene="qac"
                     /locus_tag="KFV31_RS00040"
                     /old_locus_tag="KFV31_00040"
     CDS             complement(4321..5865)
                     /gene="qac"
                     /locus_tag="KFV31_RS00040"
                     /old_locus_tag="KFV31_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacA/B family quaternary ammonium compound efflux
                     MFS transporter"
                     /protein_id="WP_219045184.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTIFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="qac"
     gene            6044..6610
                     /gene="qacR"
                     /locus_tag="KFV31_RS00045"
                     /old_locus_tag="KFV31_00045"
     ARG             6045..6607
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6861..7421
                     /locus_tag="KFV31_RS00050"
                     /old_locus_tag="KFV31_00050"
     CDS             6861..7421
                     /locus_tag="KFV31_RS00050"
                     /old_locus_tag="KFV31_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002504693.1"
                     /translation="MAKIGYARVSTQDQSLDGQIDTLEEYGCERIFSEKASGRKTKRTE
                     LDKCLDYLREGDILVVYKLDRLGRTTKQLIELSQWLDENSIDLHIIDMNVSTKDAMGKM
                     FFTMMSAFAELEANLLSERTKKGLEAARARGRKGGRPSLPDHKKREIKFLYDEQKLSGE
                     EIAEQTGVSRSTVYRIIKESRKI"
                     /name="recombinase family protein"
     gene            7840..8751
                     /locus_tag="KFV31_RS00055"
                     /old_locus_tag="KFV31_00055"
     CDS             7840..8751
                     /locus_tag="KFV31_RS00055"
                     /old_locus_tag="KFV31_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000176962.1"
                     /translation="MTLVCTKFNHDAKSTDYLNISIEEDTLQYIDPNLIKVISNREVGK
                     VATLAQRAQEQIDVFFEKIIDIHLSDFSDTQKRNEFRKLFSHFSEPHHLRLGFSQKGNT
                     GKGTTASELINLFMNEDILSIILNDDGLTIPQKAPLIRNFGDDKLSDLTSNIIMNVIID
                     FNKSMIQSFPEMKSFLSKETVTYYYFSTNGIWETYDFTPFLFDGNKTLLVPKLFTTYNQ
                     TSSLDLMIRVYIEEEIIRLQKQRNTTKKMTKKQFINEFIKGHRMDNMTYFFLNTSSKSH
                     KKFQKELDSKANERGNNNKDDN"
                     /name="hypothetical protein"
     gene            complement(8928..9026)
                     /locus_tag="KFV31_RS00060"
                     /old_locus_tag="KFV31_00060"
     CDS             complement(8928..9026)
                     /locus_tag="KFV31_RS00060"
                     /old_locus_tag="KFV31_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_000491388.1"
                     /translation="MFSIIFVSIVAPIIVGVIITLFSHWLNNRDKQ"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9190..9888
                     /locus_tag="KFV31_RS00065"
                     /old_locus_tag="KFV31_00065"
     CDS             9190..9888
                     /locus_tag="KFV31_RS00065"
                     /old_locus_tag="KFV31_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175396.1"
                     /GO_component="GO:0009986 - cell surface [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing protein"
                     /protein_id="WP_000747403.1"
                     /translation="MKKRSLLPTLLLASSLTFTGFSATANAEDGAKTVYGENLPNSADT
                     NGDGWANVDFNTSSLPQSAQNQISELSKQKDSGELTQIEYNEKVSSVFNEYQSQSTDIQ
                     NNERTQQGKRPFGGVIPNCMTQEEYQELESHVPNPNSVSTEKYNQIVKNESQKIANEEN
                     KTINTPQGVLTPVTENKNKSSEGMKNQDSNSQVLPETGESNQSDILSIIGASGLLILGI
                     SLLIFRKKAI"
                     /name="LPXTG cell wall anchor domain-containing protein"
     gene            10039..10743
                     /locus_tag="KFV31_RS00070"
                     /old_locus_tag="KFV31_00070"
     CDS             10039..10743
                     /locus_tag="KFV31_RS00070"
                     /old_locus_tag="KFV31_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000720620.1"
                     /translation="MKKIILPILIVSLSVILVSCNNNDSEHHNDNNSSDTNTSENKDNE
                     ASNEIDEKTNTSDSADNSESSNKQNKEKNDNKKQEKNNDSNKKQQSTDKNKSYLNNFNS
                     KEIEYARVWYQLISTRNDLKGIKNVYVTEIPKGSKVNPQAKNSAIYKEKVVKLEAPMRA
                     GGSVTYSSNGDGTINVYNNIPYKWENSQNSDYSQMNKITKKAIEENIETVYIKPYDNKT
                     VAKLASKIRYNK"
                     /name="hypothetical protein"
     gene            complement(10792..11025)
                     /locus_tag="KFV31_RS00075"
                     /old_locus_tag="KFV31_00075"
     CDS             complement(10792..11025)
                     /locus_tag="KFV31_RS00075"
                     /old_locus_tag="KFV31_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001091620.1"
                     /translation="MNTFVFGVITKRLEEIGFNVHKVFKMPEKQALFEDLYHYVRYVKP
                     CKAELTMVALDSDELIHYFICCYMERYAYLLN"
                     /name="hypothetical protein"
     gene            complement(11102..11515)
                     /locus_tag="KFV31_RS13690"
                     /old_locus_tag="KFV31_00080"
     CDS             complement(11102..11515)
                     /locus_tag="KFV31_RS13690"
                     /old_locus_tag="KFV31_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="WP_000448282.1"
                     /translation="METKIGHQIQKLRKSKNMSQEKLAEKLGVSRHSISNWEREVSNPD
                     LKTILEITKLFNVSLNQLIKGVEIMQVNKYVYSALAFFLGGFGAHKFYVKKNKNALLYL
                     LFCWTGIPGVIGMVEGVLTLMRPSDSENNIEIN"
                     /name="NINE protein"
     ARG             complement(4325..5865)
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.81"
                     /coverage="100.0"
     Typing          complement(1101..2035)
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11859..13570)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 agtatgattt ctaaacttaa ttttttcatc tgtacgttcg ctatataatg cttcatcaga
       61 acgataagac gctgacgcaa cgatattttt tgttgctcga ttaatgttag tcactctcaa
      121 cataaagtaa cctttattca tgattttacc tcctctcttt tctgtttttt tacccctgtg
      181 ggaactataa tgatcaacca gtatggttgc ccatttttat gccaattttc aatttgcatg
      241 taaatgggca gtgcagtgtc ttaagaactg gttatattat accatggatg tgcgacttac
      301 ccaatgaatt atggtatgct ttatatagat aaactaaggg agttttttaa tgtctaaaat
      361 taaacaaaca gatattacaa aattaagtca attagaaaaa caaattgcac aactgaaaga
      421 gcaaaagaaa aaacaagaag aacaactatc taataaaatt ggtggttatt ttttaaataa
      481 acttgattta aatatgatca actctagtga tgaattgtat aaaattatgg ataaagtcat
      541 tggagaattt aataaagata tcgaacaaaa tagcagcaac gagaacaagg aaaatttaac
      601 agaagacaaa attaactaga atagtaagtg atacgatgaa tgattttaaa catctcaaaa
      661 aaataaatgc tgaaattgaa aaagcagaat taagaaagca tagattaaaa aatttagata
      721 gaaaagaaag agcacataga ctgattcgaa aaggtgcaat gcttgaaaag tattttgaat
      781 gtgagcattt aagtcctgat gaaacagaag aattgttaaa gatgtattcc aactacatta
      841 atacaaataa acccaataaa tataaaaaga agtaatttat catgcttaca aatacttggg
      901 tttcactaaa aagaaaaaat tgctgatagt agtaacaatg aataataatt gtttataaaa
      961 atgggactaa aaaagaaaag ttttagtccc acttacttaa ttaattacat ttcattgaaa
     1021 ataaagttcg cttacagtta agagaacatc taattaaaga tattctctaa ttggatcatt
     1081 gttggcttat taagttgtat tattgattaa ataaagaagt gattggtaaa tgatcacgtc
     1141 gtttattaag cgtatgtact tctgcaaatt cagatttaag agatttcatt aagtaccctt
     1201 gcattgcttc aacggtttca tttttttgaa ttaatgtagc tttaaaacgc ttaagaacat
     1261 taacaatttc aaattccata tcttctaata aataatatgc atcaatagaa ttgttgaagg
     1321 atgttttggc ttttaataac acagatttaa taagtttaat ttcagataca gaaaagttac
     1381 ttaaataact ttgaatactt tgtggtagtt cttcaagtaa ttgaaatttt aaagcttcat
     1441 cattgaattt tgaatagtag tgatttgaat gatttgtatg attactagaa tatgttaatt
     1501 tcctatcatt caaatcatac atatcattat aattagtctt aatataatca gtgtcattat
     1561 aatcagtatc attagactgt gaatttggaa gttcttgacc acttaaattc acagttctag
     1621 aacttccaaa ttcattgttc tggaactgtg aatttggaag ttccttgata tgactggctt
     1681 caatgtcata attaggtttt aataggtata atctatttgg tttattaagg ccaacacgtt
     1741 cttgagttag taaattaaat tttattaact ctttttttaa ttttataatt ttttgttctg
     1801 cataacctaa gtattcacac aaatgtttat tactgaaaat aaagtaaata ttattatttt
     1861 tatctaccca ttcattttgg agggataatt caaaacgatc ttttaataac gcatacataa
     1921 ttttggcttc tgcagacatt ttcttaaaat atgcatcttc aaataaatat ttaggcagtt
     1981 gataaaatct ttcagttttt acttgtgata aattatattt ttcaaaattg ggcataataa
     2041 aaacctcatt tccattttgg gattgaggta ataaaacaat ttttacgtaa gttatatgtt
     2101 ttgtgcttgc acttagatga ttagatatat ataatacaag tatcaaaaaa atatataaac
     2161 aaacgagtgc gaatcgtttt attaccgaga gtcgatgcta cgccaatagc atcgactctt
     2221 ttaatattta taaagtaata ttaacacttt taatggcgta tataaagagt tttaaatgta
     2281 attaaaagta cacctaaaac taccaaacgt ataattcgct attttagcac ctaattaagt
     2341 ggcgaaaata ttttaaggat agataattag gcatttaggt agttaataga tagtaaattt
     2401 agtactaaat gatgaaataa attttgttag gtggtaaaat ttaggtacta aattaggtac
     2461 ctaagggggg atgttatgaa aactattaaa atgattgctg atgaattagg tgtaactaaa
     2521 caaactattg ttaataatgc taaaagatta aatatatctt ttgaaaaaga aaatggaatt
     2581 aattatatta aagataatga ttatttaaaa attatagaaa aaatcactaa gaaagagaaa
     2641 acaactcata ataaagaatc agtaaaaaat gaattattta atgaaaatac tgcaaaagtt
     2701 gaaaagtata tacataataa ttcaaatagt tctgagacat taaaaacaaa agtaaacgaa
     2761 ttagaaaagc aaatagaaat ctttgaaact agagctaaaa atgatgaaaa atatattgag
     2821 aatctaacta aacaattaga tcaacaaaat agcaatgtta atacattaaa taaactgttg
     2881 gagaatcaac aaatattatc tttagaaaac aataaaaaaa ttcaaaaact agaaagccaa
     2941 ttagaagaag aaagacagct taaatattct tttgatacaa cgatgaataa tagacagaat
     3001 attaatccac aagaagcaac ttttactgaa gaatctcaaa atatcaacca acaacaaaaa
     3061 gaatgtcaac caaccgaagt aaatcataaa aatataactg aagagaataa gcatgacagt
     3121 acagatgaat taacaaaaga ggataagttt gttgaaaaaa cgaagaacga ttccagtgat
     3181 aatagggagg tagaaccacc taaaaagaga ttttggagtc gtttatttgg taactaattg
     3241 caattaaaaa ggcacacaag ttcatgaact tgttgtgcct ttttaatgtg aatcaaactt
     3301 ctgataatca tcagttatgt taaactctaa caaaaactaa attatcagaa gaattatcta
     3361 gggtattaaa cataggtaaa tcaattttct atcgccaata gtataaacat catccatatt
     3421 aatctgaaga tagctacata attctttaac agcatagcct ttatttgtat aaggcgccat
     3481 aaactctatt aaatatggtt tgttttgagg tttctatgtt taatcattta atttttcatt
     3541 atataaatcg aataattata aaaaatcatc atacttttta ttttttaaaa atatttgaaa
     3601 atttaatagt aatccaatta taagatcaaa tatcattatc attataatca aggcaactat
     3661 aatcactgtg acactcgtta cataacaagc tagtaatctc cagataaaat tgacattttc
     3721 accaattcca taacttaaca tattacaaag tagtaaaaga ataataatac ctatactgta
     3781 aattaaaatt gtcagtgtat attttttaat aactctatta ggaaacaata gttcatctat
     3841 taggttcttt ttcaccagta tctctcgttc aattctatct ttgtcacaga ttttgatttt
     3901 actatttttt attttttcct ctaataaaat aacctcgaac gatctgctaa tttttaataa
     3961 atatgcagga aaagctaatg ttaagcataa tgtaattaat tcatttacat tgttcatgta
     4021 acttataaaa tagatgaaag ttgctactag tgctcctaaa aatatagtta tattaagttc
     4081 tacatcaaat attttaaaaa tatctgctct attcatcagt taatcatcct ttcaaggttt
     4141 attaatacta ataaattatt agatataggt atatcatatt attaatttaa gaaattgtct
     4201 tttataattt tacttaataa taaaaaagta gaaccattta aaattaatgg ttctactttt
     4261 taactattac taattttaaa aataaactaa tctacaatat ctaaaaatat atgtttagta
     4321 ctatttttgt ttagttatat caagtgattt gggaattaac aaatagacaa ctattgaaat
     4381 gataatcatg ataatcccac ctactaaagc agttgctaca aaagcatcat taaatgatgt
     4441 tacagcctcg tttgcaagct gttttatccc cgtagcttta gctacttcga cagcgcccac
     4501 tacagattct tcagctacat gagctaaatc tccaactata ccttttgatg aaaaagatga
     4561 aatatctaaa aatacacgat aaagcataga agataggcta ccaagtactg ctacaccaaa
     4621 aacatttcca aggtcataca tagactcttc aacagcagct gcattacctg cttttgatgt
     4681 aggtgtttct aacattatta gagcagatgc aactgctagt gaagccatac cagctccaac
     4741 taaaattaat gctaaagcca ttgtagaata tgataatgga tgaccaaaga aatacataat
     4801 aaacatgcca atggctgcaa ttccaattcc ggaaggtaac actattttcg gtccaaatcg
     4861 cgccgctaat ccgggtgcaa ttggtgcaaa caccatatct cctattgcca taggtaatag
     4921 gtataagcca gctttaaaag gagaaagttc ttccacaacc tgtaaccatt gtgaagctaa
     4981 taacaaaaca gatgccattg caaaaattgt cataaatgca gcaattgtac cagctgaaaa
     5041 tgatctcttt ttaaaaagtc ttacgtctaa cattggatca gaacttgata aattacgttt
     5101 aacaaatatc actatcatgg taattgctaa tactattaca acccatggaa taatatctgc
     5161 tagtccttct tttgaaaatt ctttgatact ccataccagt ccaatcatgc ctgcaattga
     5221 taaaattgta gaaggaatat cccacgagtg agacttttct tttgataact tagactctgg
     5281 taataaaaat aaacctgcta caactgctat tatcgcaaac ggtacattaa ttaaaaatgc
     5341 cgagtgccat gaaaattgct caagtaaagc tcctccgata attggtccaa aaacagcacc
     5401 tatcgatgaa gcgattgacc atacagctaa tgcagtggcc ctttctttag ggttttcaaa
     5461 aattactctt atcattgaaa gagtagttgg cattattaaa gcacctgcaa taccaagtaa
     5521 aaatcgaata gctattacga actctgcact ttctgcgaaa aatatagcta atgaaacgag
     5581 gccaaataaa gcaaatccag ttaataatgc tttttttctt ccccatttat cagcaaaggc
     5641 actcaatgga attataaagc cagctaaaac aagagagtat atatcaacta tccataactg
     5701 ttgggtacca gaaggctcta actctcttac taattccggt aaagccataa ttaatattgt
     5761 catatccatt gtaacaacaa acaaactaac agctaatact actagtgcag tccatctttt
     5821 ttttgatgtc atcatatcag tagtttttgt aaaaaatgaa atcatttaag ttccctccaa
     5881 tccttataga ccgtgcgatc ggtctataag gattataatc tatttttttt acatattaca
     5941 acaaaaaatc ttgcgaaatt taaatggctg tattgtgtta tacaattatt ctttgaattt
     6001 tttgtgctat cattgatagt acttaatact cattaaaggc gtgatgaact tgaaagataa
     6061 aatactaggt gtcgcaaagg aattatttat aaaaaatgga tataatgcca ctactactgg
     6121 agaaattgtt aaattatcag aaagtagtaa agggaatctt tattatcact ttaaaacaaa
     6181 agaaaatcta tttttagaaa ttttaaatat agaagaatct aaatggcaag aacagtggaa
     6241 aaaggaacaa atcaaatgta aaactaatag agaaaaattt tatttatata atgaactatc
     6301 tttaacaacc gaatactatt atccacttca aaacgcaata attgaatttt acactgaata
     6361 ttataaaact aatagcatta atgaaaaaat gaataaatta gaaaacaaat atatagatgc
     6421 ttatcatgta atttttaagg agggtaattt aaatggcgaa tggtgtatta atgatgttaa
     6481 tgctgttagt aaaatagcag caaacgctgt taatggaatt gttaccttta cacatgaaca
     6541 aaatattaat gaaagaatta aacttatgaa caagttctct caaatttttt taaatggact
     6601 tagtaaataa ttatatatat attcttgaaa taccaataaa tattatttct aaattacgtt
     6661 cgtttttgac acaaaacctc ttaacttaat attaagaggt ttttcttgga tatttaagtc
     6721 tataaacttg tgttataatc aatgtatcat aaatatcgaa aatcatgagt ttttagatgc
     6781 tgacagacgt gtttatttac atcgagaact atatttcatt agtaagtacc cataaaacga
     6841 ttaaaaaagg tggtgttcaa atggctaaaa ttggttatgc acgtgtgtca acacaagatc
     6901 aaagtcttga tggacagatt gatacacttg aggaatatgg ttgtgaacgt atctttagcg
     6961 agaaagcgag tggtcgtaaa actaaaagaa cggaacttga taagtgttta gattatttac
     7021 gtgaaggaga tattctagtt gtctataaat tagatcgtct tggacgtaca acaaaacaat
     7081 taattgagct atctcaatgg ttggatgaaa atagcattga tttacatatt atagatatga
     7141 atgtgtcaac gaaagatgcg atgggcaaga tgttctttac catgatgagt gcattcgctg
     7201 aacttgaagc taatttatta agtgaacgaa ctaaaaaagg tttggaagct gcaagagcaa
     7261 gagggagaaa aggtggaaga ccttcactac cagatcataa gaaaagagag atcaaattct
     7321 tatatgatga acaaaagctg tctggtgaag aaattgctga acaaacagga gtgagtcgtt
     7381 ctactgtata taggattatt aaagagtcaa gaaaaatata aagtactaaa ttaaagtttt
     7441 aatataccct ttaattgaaa aatcttctta aaaagtgtct ttaacttcag tcccctttaa
     7501 caatccactc gcttaatagc tttaaatttc taagcattac ttgagctttg aaaattttca
     7561 tcgattcatt aaccgacata actggatttt gactgttaat attcaccttg tcctctccaa
     7621 ttaacaaaaa agcaaaagca tataaacaac atttgttgtt tatatgcttt tgcttgcatt
     7681 ttcttctata gtatgctata atgaaaaggt taagtctatc tatacatcaa aagcaataaa
     7741 cttccaccta gaataacaac ttattgtaaa tcgacaatta caataataca tcattctata
     7801 ttggaagtaa acaaaaaact tacagaagga tgatatagag tgacactagt atgtaccaaa
     7861 ttcaatcatg atgctaaatc aactgattat ttaaatattt ctattgaaga agacactctt
     7921 caatatatag acccaaacct tattaaagta atatctaata gagaagttgg aaaagttgcg
     7981 actttggctc aaagagcaca agagcaaatt gatgtttttt ttgaaaaaat tatcgatata
     8041 catcttagtg atttttctga tacccaaaag cgtaatgaat ttagaaaatt attctctcat
     8101 ttttcagaac cacaccattt acgcttaggt ttttctcaga aaggtaatac aggaaaaggt
     8161 actacagcat ctgaattgat taatctattt atgaatgaag atattctttc cattatctta
     8221 aatgatgacg gattaacaat acctcaaaaa gcaccattaa tcagaaattt tggtgatgat
     8281 aaattatcag atttaacaag taatataatc atgaatgtta taattgattt taataaatcc
     8341 atgattcagt catttccaga aatgaaatcg tttctttcta aagaaactgt gacatattat
     8401 tatttctcta ctaacggtat atgggaaact tatgatttca ctccatttct ttttgatggt
     8461 aataaaactt tattagttcc aaaattattc actacttata atcagacatc tagtttagat
     8521 ttgatgataa gagtatacat tgaagaagaa atcattagat tgcaaaaaca acgtaataca
     8581 accaaaaaaa tgacaaaaaa acaatttatc aatgaattta taaaaggaca tcgtatggat
     8641 aatatgacct atttcttttt aaacacttct tcaaaatcac acaaaaaatt tcaaaaagaa
     8701 ttagattcaa aagccaatga aaggggtaac aataacaaag atgataatta gtacttacta
     8761 atcagtatag tatagactta acatactaat tattcaattt tgcattcctc cacactatga
     8821 tgttataatt tagataacaa atatgtgttg aacatggtaa acaccgagcc cgataactat
     8881 tccagtagtt atcgggctct ttttggtgtt attactgtgt cgtcgcttta ttgtttatca
     8941 cgattattca accaatgtga aaacaacgtg attataaccc ctactataat aggagctact
     9001 atagatacaa atatgatgct gaacatggtg tcacctcctt ccatgctaaa ccagcacaga
     9061 agtagaacga cgacttttat attatatcat ttgtgaattg ttaagcatat gcacttccga
     9121 taagtcattt tatgtttaat gtatctatgt gataatgaaa atacaaattc tattaaagga
     9181 gataatttta tgaaaaaacg atcattactt ccaactttat tattggctag ctcattaaca
     9241 ttcacaggtt tttcagcgac agccaatgct gaggatggtg ccaaaaccgt atacggagag
     9301 aacttaccaa atagcgcaga tacaaatgga gatggttggg ctaatgtaga cttcaatact
     9361 tcatccctcc cacaatctgc acaaaatcaa ataagtgaac tttctaaaca gaaagattca
     9421 ggggaattaa ctcaaatcga gtataatgag aaagtttcaa gtgtatttaa tgaatatcaa
     9481 tcacaaagta ctgatataca aaataatgag agaactcaac aaggtaaaag acctttcgga
     9541 ggggtgatac ctaattgtat gactcaagaa gaataccaag aattagaaag tcatgttcct
     9601 aaccctaata gtgtatccac agaaaaatat aatcaaattg taaaaaatga aagtcaaaag
     9661 atagctaatg aagaaaataa aactattaat acacctcaag gtgtgttgac acctgtaact
     9721 gaaaataaaa acaagagttc tgaaggaatg aaaaatcaag attcaaactc tcaagtatta
     9781 ccagaaacag gagaaagcaa ccaaagtgat atattgtcaa taattggcgc ttctggacta
     9841 ctaatattag gtataagtct tttaatattc agaaaaaagg caatataaaa attgataaaa
     9901 taaccaccgt gtttatgtaa aacatgtggt tattttatta tatatttaat acatattttt
     9961 caactcatgt aatttacgtt ttcttgctga aaaaatatta acataatcta atttgaaata
    10021 ctgaaaagga gttgtaatat gaaaaaaatc attttaccta tattaattgt tagtttatcg
    10081 gtaattttag tgagttgtaa taataacgat tcagaacatc acaatgataa taattctagt
    10141 gatactaaca cctcagaaaa caaagataat gaagcatcaa atgaaattga cgaaaaaact
    10201 aacacatctg atagtgcgga taattcagaa agtagtaata aacaaaataa agaaaaaaat
    10261 gataataaaa aacaagaaaa aaataatgat agtaataaga agcaacaatc tactgataaa
    10321 aataaaagtt acttaaataa ttttaactcg aaagaaattg aatatgcacg agtttggtat
    10381 cagttaatat ctacaagaaa tgatttaaaa ggtattaaaa atgtttatgt tacagaaata
    10441 ccaaaaggct caaaagtaaa tccacaagcc aaaaatagtg caatttataa agaaaaagtt
    10501 gtgaaattag aagctcctat gagagctggt gggtctgtaa catatagcag taatggtgat
    10561 ggaacaatta atgtatataa taatatccct tataaatggg aaaattcaca aaatagtgat
    10621 tatagtcaaa tgaataagat aacaaaaaaa gcaatagagg agaatattga aacagtctat
    10681 attaaaccct acgacaataa aacagtagct aagttagcaa gtaaaattag atataataag
    10741 tagttagtag attattaaaa aggcgcctaa aattaggcgt cttttttatc attagttcaa
    10801 aagataagca taacgttcca tgtaacagca tataaagtaa tgaatcaatt catcactatc
    10861 taatgccacc attgttagtt cagccttgca tggtttaacg tatctcacat aatggtacaa
    10921 atcttcaaat agtgcctgtt tttcgggcat tttaaacacc ttgtgtacat taaaacctat
    10981 ttcttctaat cgctttgtaa tcacgccgaa aacgaatgtg ttcatgtgaa atcactccat
    11041 catttttaat attcacatgt acactaaaaa aaatccgata catcggcttt ttatttttgg
    11101 tttaatttat ttctatattg ttttcactat cgctcggacg cataagtgtt aaaacacctt
    11161 ctaccatacc tatcacaccc ggtattcccg tccaacaaaa tagtaaataa agaagagcat
    11221 ttttattttt cttaacataa aacttatgag ccccgaaacc tcctagaaaa aatgctaatg
    11281 cagagtaaac atatttatta acttgcatga tttccactcc tttaattaat tgatttagag
    11341 aaacattaaa taatttagtg atttctaaaa ttgtttttaa gtcgggatta cttacttctc
    11401 tttcccaatt agaaatagaa tgtctcgata cccctaactt ttcagctaat ttttcttgag
    11461 acatgttttt actttttctt aacttttgta tttgatgtcc aatttttgtt tccaaattat
    11521 ttttctcctt aactttaaga tatatttaaa ctcaattttt tctatcgata tttatgaaca
    11581 ttagtatttt ttatgctgaa atatgtgaac aatcgcataa tatcaaggtt tttctataat
    11641 tatacacgtt ttaaaataca ttgtgataat acatagaatg taccttatct aagtatataa
    11701 atgttttctt taaaccaaag ctaatttacc acattgggtt aagtttctta ttattaaaga
    11761 atgaactttg tatgatttca actgcttact atcttctcat ttggttattt ctctcttgtt
    11821 tcttcttctt tttctgatga ttaatattgt tttgcttttc agtttcgttc gcatagtaca
    11881 taagaaagtc actagcattt atcgttggta aattaatgtg attagtttgt tcattttcat
    11941 gttcaatacg attgtcatga tcatttctat ctacttgatt aatatgtttt tcgataactt
    12001 cttcttgatc gacatgatta tatttctcaa gtttaatgag taattgaact ttttcattag
    12061 gtgtttggtc tttgactttt tcaaagtttt tatcgcctat atctattctt aaatcatcat
    12121 caatatgttc tttataacta tcaatttctt tttctttttc cttaataaat tgcaactcaa
    12181 cgttatattc aaagctatct ttttctaaat ttttaagatg tttttgacta aagaaaatat
    12241 ctttactcgc ttttttataa gtatcttcta gcaatacatc attaggaata ttgttatttt
    12301 gtttgaacag gtattgaaca tgagttgctt ttataatatt acctttttgt gcctcatcaa
    12361 caataaatgc tttttcatca tggctaaaat gatgatgaga tttagggtac atttgctgaa
    12421 caatgagatg atttttttca ctttcagtta gataaacatc tttagctgat tgataaatct
    12481 ctttacgttt ttcctctcta ctttctaatt tattcttctc attgagtaac gctttatagt
    12541 tagtttcaac agtattaaca aactcttgtt gactatatcc tttactttca atttcttttc
    12601 taaactgata attttcatca taatttttag catagaattt taattgacgt ctcttaattt
    12661 catttttaag taaatcattt tgaattttac gaccaaaggt agacgcactg tcaaacgtat
    12721 ttttaattgt ttctttcgcc tctgaatagc caacatcacg ttgatatcta tcaaataaaa
    12781 tagaataact ttgttcattt ttacttactt gttcgttagc attttgaata aataatctaa
    12841 tttcattttg tgcttgttct ttctctttat cactaaataa ttgtgatact tcgccacgat
    12901 tatatcttct aatttgttca tttttctgtc cgtaataagt aacgggttgg tactcgatac
    12961 cctctttttc acagcgtttt ctttcatttt gttctaatct atattcttct cttgtaagtg
    13021 gaatttcggc aattttttct aaaccttgcg cttcaaaaga acgactatca tatcgtctta
    13081 aactttgttc acgttctgag tattgattta attttaattc ccaatgctta cgaacttctt
    13141 tgaaatcaaa gtcggcaaat cgttcttgtt ttctaacttt attaccattt ttatttaaaa
    13201 tcgcattgcc attttcatca tattgcgttc tcgtttttgt ctttttcccc caagtaccgt
    13261 ctgcattaaa cggtctttga gtaagtagaa catgggcgtg tgggttgttt atatcatctc
    13321 tatgaattga aatatcagca accattcctt tgtctacaaa ctcacttttt ataaaatctt
    13381 tagttaattc agtttgtacc tctctatcaa aatcattagg caaacttaat aagacttctt
    13441 ttgctaatct aggatttttt gttttcgcat tattcttttc aactttatca acttcattcc
    13501 ataaacgctg tctatcttta gtccattcgg gtgcatgttg aggagttaaa atcatgcttt
    13561 caggcttaat
//
